Amount Of Linoleic Acid Research Articles

Physicochemical properties, profile of volatiles, fatty acids, lipids and concomitants from four Kadsura coccinea seed oils

The Kadsura coccinea fruit is a wild fruit that may be eaten and used medicinally. Its seeds are rich in nutrients but are typically thrown away without processing.The physicochemical characterization, volatiles, fatty acids, lipids and concomitants of cold-processed seed oils from four kinds of K. coccinea were evaluated. The average kernel yield and oil yield of K. coccinea seeds were 68.21 % and 30.44 %, respectively. The seed oil contains a moderate level of total phenolics (368.99–503.99 mgGAE/100 g), total flavonoids (95.01–126.18 mg RE/100 g), and β-sitosterol (1498.8–1712.7 mg/kg) with higher iodine value, lower acid value, saponification value and shorter induction time. GC analysis reveals appreciable amounts of linoleic acid (64.91–68.05 %) and squalene in seed oil. GC–MS analysis showed that the major volatile compounds were γ-muurolene (27.25–31.7 %), β-himachalene (19.51–20.37 %) and β-curcumene (15.78–16.78 %). Moreover, 16 terpenoids, 14 phenolics were identified by UPLC-QTOF-MS/MS. These results suggest that K. coccinea seed seems an promising alternative oilseed with biological ingredients for food, cosmetics and pharmaceutical industries.

Phenolic profile, fatty acid and mineral composition with antioxidant, antibacterial, and enzyme inhibitor activities of different extracts from Erodium Cicutarium (L.) L’Hér. consumed as a vegetable in Kilis, Turkey

This study aimed to explore the antioxidant activities, phenolic profile, fatty acid and mineral composition of the different extracts of Erodium cicutarium (L.) L’Hér. consumed as a vegetable in Kilis, Turkey. While catechine hydrate was the most abundant phenolic compounds in methanol, ethanol and diethyl ether extracts, it was chlorogenic acid in the water extract. Looking at the fatty acid profile, the amount of palmitic acid, one of the saturated fatty acids, was found to be high (34.30%), followed by stearic acid (5.10%). Total monounsaturated fatty acids are the second highest fatty acids. Total polyunsaturated fatty acids were determined as the third rank fatty acids. While the amount of linoleic acid, one of the total polyunsaturated fatty acids, was determined as 17.62%, the ratio of linolenic acid was determined as 9.60%. While the most calcium, magnesium and potassium were found among the 9 different mineral substances determined, respectively; the lowest element was found to be nitrogen. Looking at the results, it was determined that the plant is a high source of calcium (1078.503 mg/kg). Inhibitory effects against Pseudomonas aeruginosa ATCC 9027 were observed, with a zone diameter of 5.5 mm and 11 mm in methanol extract and diethyl ether extract, respectively. Ethanol and water extracts of E. cicutarium (L.) L.‘Hér. may be preferred as an alternative natural agent due to their amylase and tyrosinase activities. The results suggested that the E. cicutarium (L.) L.‘Hér. extracts may be useful for food and medicinal applications.

Corn Oil Oleogel Structured with Chicken Skin as A Potential Fat Replacer in Meat Batters

This study investigated the effects of replacing beef fat with corn oil chicken skin oleogel on the quality parameters of model meat batters. Four different batches were prepared with varying amounts of oleogel (50% (O50), 75% (O75), 100% (O100)) as a fat substitute, while a control group was prepared with 100% (C) beef fat. Chemical composition, technological properties, color, texture, fatty acid composition, and oxidative changes were evaluated. The results showed that oleogel addition increased moisture and decreased protein content. Water holding capacity and cooking yield improved by the addition of oleogel. Replacing beef fat with oleogel increased lightness and yellowness, and reduced redness compared to control samples. The hardness, gumminess, springiness, and chewiness of the control were significantly higher than the samples formulated with oleogel. The incorporation of oleogel resulted in a reduction in saturated fatty acids (SFA) and an increase in polyunsaturated fatty acids (PUFA). As the level of oleogel increased, the amount of linolenic acid also increased significantly. Replacing beef fat with oleogel resulted in significant reductions in the atherogenicity (IA) and thrombogenicity (IT) indexes. At the end of the storage, the highest TBARS value was recorded in the control sample formulated with 100% beef fat.

The role of germinating, roasting and boiling on bioactive properties, fatty acid, phenolic components and biogenic element contents of soybean seeds

SummaryIn this study, the effects of germination, roasting and boiling processes on bioactive substances, mineral profiles, fatty acid composition and phenolic profiles present in soybean seeds and soybean oil extracted from soybean were investigated. While the acidity value of the crude and roasted soybean seed oils are 0.89% and 1.09%, the acidity values of the germinted and boiled soybean oils were 1.58% and 1.21%, respectively. The free fatty acid value of the germination sample group was established to be higher than the other treatment groups. The amounts of total phenolic substance were reported between 10.22 and 33.78 mgGAE/100 g. Linoleic acid amounts of soybean oil varied between 55.72% and 56.14%. The total carotenoid and flavonoid amounts of seeds were measured to be between 0.53 (control) and 2.83 μg g−1 (germination) to 43.60 mgCE kg−1 (boiled) to 126.60 mgCE kg−1 (roasting), respectively. Protein results of the seeds were established between 43.94% (control) and 46.94% (germination). The amount of K ranged from 10 883 (boiled) to 13 994 mg kg−1 (roasting), while the amount of P range from 5340.8 (control) to 5695.9 mg kg−1 germination. The amount of Ca of the samples varied between 4606.1 (control) and 5216.1 (boiled). While linoleic acid is determined at the highest rate in soybean oil obtained from crude soybean, linoleic acid amount was determined as highest in oil obtained after roasting, germination and boiling processes applied to soybean rutin contents of soybean seed samples were determined between 3.52 and 7.81 mg kg−1. Boiling and germination increased the routine value in soybean seed samples and was determined as 7.62 and 7.81 mg kg−1, respectively. Roasting, germination and boiling processes reduced the cinnamic acid values of soybeans.

White mustard (Sinapis alba L.) seeds: the role of oven and microwave roasting on their bioactive components, antioxidant potential, fatty acids and mineral contents

The oil amounts of raw and roasted mustard seeds were reported between 12.05 (control) and 16.30% (oven). Total phenolic and flavonoid amounts of untreated (control) and roasted mustard seeds were recorded to be between 473.90 (control) and 569.96 mgGAE/100 g (oven) to 345.48 (control) and 479.76 mg/100 g (microwave), respectively. Antioxidant capacity results of untreated and roasted mustard seeds were determined to be between 6.71 (oven) and 6.97 mmol/kg (control). Total phenolic and flavonoid amounts of oven-roasted seeds were higher than those of control and microwave-roasted seeds. L* values of mustard seeds varied between 35.04 and 65.52. Heat treatment caused a decrease in L* values. The lowest L* value was observed in the sample dried in conventional oven. The quantitative values of gallic and 3,4-dihydroxybenzoic acids of mustard seeds were recorded to be between 8.47 (control) and 117.31 mg/100 g (microwave) to 2.16 (control) and 11.79 mg/100 g (microwave), respectively. Erucic acid values of the oils extracted from raw and roasted mustard seeds were reported between 41.38 (control) and 42.81% (microwave). Oleic and linoleic acid amounts of mustard oils differed between 26.06 (microwave) and 26.90% (control) and between 13.08 (oven) and 13.98% (control), respectively. The oven roasting system had the most effect on the element and protein contents of the seeds, followed by the microwave in decreasing order. As with the bioactive components of the seeds, the amounts of phenolic constituents of roasted mustard seeds increased significantly compared to the control.Graphical abstract

Chemical Compositions and Essential Fatty Acid Analysis of Selected Vegetable Oils and Fats

The fatty acid (FA) compositions of thirty-nine vegetable oils and fats, including nangai nut, pili nut, shea butter, tamanu oil, baobab, sea buckthorn berry, Brazil nut, grape seed, black seed, evening primrose, passion fruit, milk thistle, sunflower, pumpkin seed, sesame, soybean, flax seed, kukui, red raspberry seed, walnut, chia seed, hemp seed, rosehip, almond, avocado, carrot seed, moringa, apricot kernel, camellia seed, macadamia, olive, marula, argan, castor, jojoba, pomegranate seed, medium-chain triglyceride (MCT) coconut, roasted coconut, canola, and mustard oil, were analyzed using gas chromatography–mass spectrometry (GC-MS). Vegetable oils and fats have different profiles in terms of their fatty acid composition, and their major constituents vary significantly. However, we categorized them into different classes based on the percentages of different fatty acids they contain. The saturated fatty acids, such as palmitic acid and stearic acid, and the unsaturated fatty acids, including oleic acid, linoleic acid, and linolenic acid, are the main categories. Among them, roasted coconut oil contained the greatest amount of saturated fatty acids followed by nangai nut (45.61%). Passion fruit oil contained the largest amount of linoleic acid (66.23%), while chia seed oil had the highest content of linolenic acid (58.25%). Oleic acid was exclusively present in camellia seed oil, constituting 78.57% of its composition. Notably, mustard oil had a significant presence of erucic acid (54.32%), while pomegranate seed oil exclusively contained punicic acid (74.77%). Jojoba oil primarily consisted of (Z)-11-eicosenoic acid (29.55%) and (Z)-docos-13-en-1-ol (27.96%). The major constituent in castor oil was ricinoleic acid (89.89%). Compared with other vegetable oils and fats, pili nut oil contained a significant amount of (E)-FA (20.62%), followed by sea buckthorn berry oil with a content of 9.60%. FA compositions from sources may be problematic in the human diet due to no labeling or the absence of essential components. Therefore, consumers must cast an eye over some essential components consumed in their dietary intake.

Compositions based on enrichment of cotton and sunflower oil with pomegranate seed oil

Oil was extracted from the seeds of pomegranate “Qayyum” variety in a Soxhlet extractor with extraction gasoline. By enriching the separated oil with sunflower and cottonseed oils, 6 different oil compositions were prepared in the proportions of 85:15, 90:10 and 95:5. The fatty acid composition of the prepared oil compositions was studied by the GC method. GC analysis of the composition of the prepared oil compositions showed that only punicic acid was detected in the conjugated α-linolenic acids. As the amount of pomegranate oil increases, the content of punicic acid in oil compositions increases. The amount of linoleic acid is the highest in the 95:5 composition of the oil, and decreases as the amount of pomegranate oil increases. Conjugated α-linolenic acids are not detected in cottonseed oil, and punicic acid is detected in compositions prepared with the addition of pomegranate oil, and its amount increases as the amount of pomegranate oil increases. Prepared oil compositions can be used in the preparation of various medicinal products and biologically active food additives depending on the acid content.

FATTY ACID COMPOSITION OF FIVE MUSHROOM SPECIES BY GC AND GC-MS WITH A CHEMOMETRIC APPROACH

Fatty acids are recognized as energy sources and membrane components. The biological effects of the fatty acids are composed of influencing cell and tissue metabolism, and responding to hormonal and other signals. This study was planned to characterize the fatty acid compositions of five different mushrooms namely, Cerrena unicolor, Hymenochaete rubiginosa, Inocutis rheades, Leptoporus mollis, and Polyporus squamosus naturally distributed in Turkey by using gas chromatography (GC) and GC-mass spectrometry (GC-MS). A total of sixteen fatty acids were screened in the mushroom species. The most abundant fatty acids were recorded as linoleic (10.35-65.69%), oleic (12.03-53.27%), palmitic (12.68-21.16%), stearic (2.39-4.36%) and palmitoleic (1.40-4.26%) acids in all studied mushrooms. The amounts of unsaturated fatty acids (UFAs) (60.02-80.70%) were calculated higher than saturated fatty acids (SFAs) (19.24-39.58%). The correlations or differences of the mushroom species with regard to fatty acid compositions were chemometrically investigated by using principal component analysis (PCA) and hierarchical clustering analysis (HCA). H. rubiginosa was separated with the highest amount of linoleic acid (65.69%) from other four mushroom species in both analyses.

Influence of foliar treatment with microfertilizers on the productivity of white mustard in forest-steppe conditions of the Middle Volga region

The article presents the results of studying the effect of foliar treatments with microfertilizers on the productivity of white mustard in the conditions of the forest-steppe of the Middle Volga region. The object of research was two zoned varieties Lutsiya and Raduga. The experiment was carried out in 2020-2022 on the fields of the Penza Research Institute of Agriculture, which consisted in the foliar application of microfertilizers on mustard crops. Meteorological conditions during the years of research differed in contrast. The hydrothermal coefficient varied from 0.72 to 1.40. The results of field experiments confirm the varietal response of white mustard to the use of microelement fertilizers. The most effective microfertilizers for the Lutsiya variety are Izagri Vita and Agroverm. Their use made it possible to obtain the highest seed yield of 1.61 and 1.63 t/ha and a significant increase relative to the control variant by 0.15-0.17 t/ha. Variety Raduga formed the highest seed yield (1.61 t/ha) with foliar treatment of crops with Agroverm and BlackJack. In addition, the use of the BlackJack preparation contributed to obtaining the largest amount of oil in the seeds of both varieties - 29.72% (Lutsiya variety) and 29.31% (Raduga variety). The maximum protein content in mustard seeds, 36.59 and 37.17%, was noted in the variant with Humate + 7 treatment, which exceeded the control by 1.40 and 1.23%. On the variants treated with Megamix and Humate +7, the largest seeds were formed, the weight of 1000 of which was 6.50 (Lutsiya) and 6.54 g (Raduga). In addition, foliar feeding of mustard with microfertilizers also influenced the fatty acid composition of oilseeds. The use of Cytovit reduces the concentration of oleic acid to 33.9% and increases the content of erucic acid to 30.1% in the Lutsiya variety. In the Raduga variety, the use of Izagri Vita and Gumat + 7 contributed to an increase in erucic acid by 1.0-1.1%, the use of BlackJack increases the content of oleic acid to 60.2%. Treatment of plants with Agroverm leads to a decrease in the amount of linoleic acid to 8.7 and 9.9%, depending on the variety.

A gut microbial metabolite of linoleic acid ameliorates liver fibrosis by inhibiting TGF-β signaling in hepatic stellate cells

The antidiabetic drug pioglitazone ameliorates insulin resistance by activating the transcription factor PPARγ. In addition to its blood glucose–lowering action, pioglitazone exerts pleiotropic effects including amelioration of nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH). The mechanism by which pioglitazone achieves this latter effect has remained unclear, however. We here show that pioglitazone administration increases the amount of linoleic acid (LA) metabolites in adipose tissue of KK-Ay mice. These metabolites are produced by lactic acid bacteria in the gut, and pioglitazone also increased the fraction of Lactobacillus in the gut microbiota. Administration of the LA metabolite HYA (10-hydroxy-cis-12-octadecenoic acid) to C57BL/6 J mice fed a high-fat diet improved liver histology including steatosis, inflammatory cell infiltration, and fibrosis. Gene ontology analysis of RNA-sequencing data for the liver revealed that the top category for genes downregulated by HYA treatment was related to extracellular matrix, and the expression of individual genes related to fibrosis was confirmed to be attenuated by HYA treatment. Mechanistically, HYA suppressed TGF-β–induced Smad3 phosphorylation and fibrosis-related gene expression in human hepatic stellate cells (LX-2). Our results implicate LA metabolites in the mechanism by which pioglitazone ameliorates liver fibrosis, and they suggest that HYA is a potential therapeutic for NAFLD/NASH.

Ultrasound and microwave-assisted extraction of silkworm pupae (Bombyx mori) oil and its quality characterisation

Abstract This study aimed to investigate the effect of Ultrasound-Assisted Extraction (USAE) and Microwave-Assisted Extraction (MWAE) methods on the quality parameters and yield of silkworm pupae oil. The selected key independent variables that impact the extraction process, such as power levels, liquid-solid (L/S) ratio, and time for the extraction of SPO (silkworm pupae oil), were optimised. The extracted silkworm pupa oil was further characterised for oil quality, thermal behaviour using DSC (Differential Scanning Calorimeter), colour values and fatty acid profile for its suitability for various food applications. USAE resulted in a maximum oil yield of 36.60 ± 0.20% at 400 W with an L/S ratio of 6 ml/g at 20 min pulsation time. The fatty acid profile of SPO through GC-MS revealed significant amounts of linoleic acid and linolenic acid (6.50% and 67.18%) in USAE, followed by MWAE (34.40 ± 0.20%). Further, the FTIR spectra revealed high-intensity bands ranging between 2300-3000 cm−1, associated with functional groups from long-chain alkyl groups and triglycerides. It was observed that SPO had Omega-3 and Omega-6 long chain fatty acids and triglycerides which are essential for human health. Antioxidant activity exhibited high free radical scavenging in USAE (27.46 ± 0.15%) followed by MWAE (26.63 ± 0.15%). The morphology of SEM images of USAE exhibits microstructural damage to cells resulting in the release of more oil. DSC thermograph displayed that crystallisation and melting curve temperatures for USAE and MWAE were lower than SXLE (soxhlet extraction) alone due to higher amounts of unsaturated fatty acids. SPO extracted using USAE exhibited higher oil yield with significant antioxidant capacity and good thermal properties for its application in food products.

Influence of microfertilizers of the Izagri series on the productivity of safflower (<i>Carthamus tinctorius</i>)

The results of the analysis of the influence of Izagri microelement fertilizers on safflower productivity in the forest-steppe conditions of the Middle Volga region are presented. The studies were conducted in 2020-2022 in the Penza region (experimental field of the Penza Research Institute of Agriculture). The object of the study was safflower of the Ershovsky 4 variety. The experiment consisted in pre-sowing seed treatment with Izagri series of microfertilizers. Izagri preparations were found to increase the growth force of seeds up to 1.21-1.57 cm, germination by 1.1-8.8%, safety of plants for harvesting by 0.5-6.2%. Against the background of fertilizer application, safflower productivity averaged 1.30-1.55 t/ha for 3 years, i.e. increased by 0.06-0.25 t/ha. The greatest effect was produced by Izagri Force and Izagri Vita, which helped to obtain a yield increase of 0.23-0.25 t/ha. The highest oil content of seeds was observed in the variants with Izagri Azot (29.85%) and Izagri Bor (30.04%): the increase was 2.7 and 3.0% respectively. Application of these preparations reduced seed huskiness to 37.7 and 37.9%, respectively. The largest seeds were formed in the variant with Izagri Vita - weight of 1 thousand seeds was 39.16 g. Treatment with Izagri Phosphorus and Izagri Vita gave a slight decrease in oleic acid content (to 9.94 and 9.62%). At the same time, the use of Izagri Vita maximized the amount of linoleic acid (up to 83.63%). After using Izagri Force, the concentration of this acid decreased to 81.88%. Based on the data obtained, it can be concluded that pre-sowing treatment of seeds with microfertilizers from Izagri line improves the quality indicators of oilseeds and can act as a method to increase the productivity of safflower.

Alheiras with animal fat replacement: application of a gelled emulsion based on hemp oil (Cannabis sativa L.) and buckwheat

A gelled emulsion elaborated using hemp seed oil and buckwheat flour was utilized as pork backfat substitute in a typical Portuguese meat product named Alheira. Three different formulations were prepared: Alheiras control (AC) with a fat content of 13%, alheiras where the 25% pork backfat was substituted with a hemp seed oil-GE (AH25), and alheiras where the 50% pork backfat was substituted with a hemp seed oil-GE (AH50). The chemical composition as well as the physicochemical properties and lipid stability of different samples were assessed. Reformulated samples showed similar protein, moisture, and fat content (p < 0.05) that AC. However, AH25 and AH50 had a higher amount of linoleic acid (16.29 and 22.14 g/100 g, respectively) and linolenic acid (1.75 and 3.45 g/100 g, respectively) than AC (12.71 and 0.51 g/100 g). Similarly, AH25 and AH50 showed lower saturated fatty acids (35.28 and 30.37 g/100 g, respectively) than AC (37.37 g/100 g). The substitution of pork backfat for hemp seed oil-GE did not modify significantly the physicochemical properties of samples. On the other hand, the lipid oxidation values increased by 30 and 65% in AH25 and AH50 respectively, in comparison to AC due to the use of polyunsaturated oils, which are highly susceptible to oxidation. This work established that the use of gelled emulsions elaborated using hemp oil and buckwheat flour may be a promising strategy to obtain meat products with a better healthier profile.

An integrated untargeted metabolomic approach reveals the quality characteristics of black soybeans from different geographical origins in China

Black soybeans are extensively planted and consumed in China due to their high nutritional value and numerous health benefits. However, very few is known about the characteristic metabolites of black soybeans from different geographical origins in China. In the present study, 31 black soybean samples were collected from 11 main producing provinces in China. A combined metabolomics approach using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) and gas chromatography coupled to an Orbitrap mass analyzer (GC-orbitrap-MS) was performed for the first time to comprehensively investigate the metabolite variability among the black soybeans from different geographical origins. A total of 48 differential non-volatile metabolites and 14 differential volatile metabolites were identified based on orthogonal partial least squares discriminant analysis (OPLS-DA) coupled with analysis of variance (ANOVA). Higher procyanidin B1, procyanidin B2, epicatechin, malonylated isoflavones, and β-pinene were observed in Gansu black soybeans. Guangxi black soybeans had higher amounts of linoleic acid and its oxidation products of hexanal and pentane. The black soybeans from Xinjiang and Yunnan were found to have higher delphinidin-derived anthocyanins, gamma-glutamyl peptides, and aromatic hydrocarbons. The characteristic metabolites of black soybeans from other geographical origins were also clarified. This study indicated that the integrated untargeted metabolomic approach can be a powerful tool to provide knowledge for developing specialty black soybeans.

A mutant cotton fatty acid desaturase 2-1d allele causes protein mistargeting and altered seed oil composition

BackgroundCotton (Gossypium sp.) has been cultivated for centuries for its spinnable fibers, but its seed oil also possesses untapped economic potential if, improvements could be made to its oleic acid content.ResultsPrevious studies, including those from our laboratory, identified pima accessions containing approximately doubled levels of seed oil oleic acid, compared to standard upland cottonseed oil. Here, the molecular properties of a fatty acid desaturase encoded by a mutant allele identified by genome sequencing in an earlier analysis were analyzed. The mutant sequence is predicted to encode a C-terminally truncated protein lacking nine residues, including a predicted endoplasmic reticulum membrane retrieval motif. We determined that the mutation was caused by a relatively recent movement of a Ty1/copia type retrotransposon that is not found associated with this desaturase gene in other sequenced cotton genomes. The mutant desaturase, along with its repaired isozyme and the wild-type A-subgenome homoeologous protein were expressed in transgenic yeast and stably transformed Arabidopsis plants. All full-length enzymes efficiently converted oleic acid to linoleic acid. The mutant desaturase protein produced only trace amounts of linoleic acid, and only when strongly overexpressed in yeast cells, indicating that the missing C-terminal amino acid residues are not strictly required for enzyme activity, yet are necessary for proper subcellular targeting to the endoplasmic reticulum membrane.ConclusionThese results provide the biochemical underpinning that links a genetic lesion present in a limited group of South American pima cotton accessions and their rare seed oil oleic acid traits. Markers developed to the mutant desaturase allele are currently being used in breeding programs designed to introduce this trait into agronomic upland cotton varieties.

The intensity of the cluster drop affects the bioactive compounds and fatty acid composition in hazelnuts

This study was conducted to determine how the intensity of the cluster drop effects nut traits, bioactive compounds, and fatty acid composition in Tombul, Palaz and Kalınkara hazelnut cultivars. The cluster drop significantly affected bioactive compounds and fatty acid composition while it did not affect the traits of the nuts. As cluster drop intensity increased, nut traits and bioactive compounds in all cultivars increased. Strong cluster drop intensity determined the highest total phenolics, total flavonoids, and antioxidant activity. Except for the Kalınkara cultivar, a low amount of linoleic acid was detected while high amounts of oleic and stearic acid were determined in slight cluster drop intensity. As cluster drop intensity increased, palmitic acid increased. Principal component analysis showed that the slight and intermediate drop intensity were generally associated with kernel length, oleic, linoleic, stearic, palmitoleic, 11-eicosenoic and arachidic acids. In contrast, strong intensity was associated with nut and kernel weight, kernel ratio, kernel width, kernel thickness, kernel size, bioactive compounds, and palmitic acid. As a result, the bioactive compounds and fatty acid composition, which are important for human health, was significantly affected by cluster drop intensity.

Microalgae as an aquaculture feed produced in a short light-path annular column photobioreactor

In this work, four microalgae species (Chlorella vulgaris, Trachydiscus minutus, Monodopsis sp., and Monoraphidium sp.) were selected and grown in a novel, 30 L short-light path annular-column photobioreactor (AC-PBR). The aim was to test the microalgae growth on a pilot scale and evaluate the potential use as an alternative fish feed in hatcheries based on the content and composition of fatty acids. All microalgae species reached a high biomass density between 3.65 and 5.32 g DW L−1 during a 14-day trial. The highest growth rate of 0.25 ± 0.05 d−1 was calculated for Trachydiscus culture. The highest total content of fatty acids (TFA) was determined for Monodopsis and Trachydiscus biomass as the concentration reached 74.5 ± 6.3 and 72.4 ± 7.6 mg g−1 DW, respectively. These two species contained mostly eicosapentaenoic acid (EPA; C20:5n3), the crucial FA for carnivorous fish whose concentration reached the value of 25.3 ± 0.9% and 31.9 ± 2.0% of TFA for Monodopsis and Trachydiscus, respectively. A high amount of linoleic acid (LA; C18:2n6), another important FA for freshwater aquafeed, was found in Chlorella biomass up to the concentration of 21.9 ± 1.5% of TFA. The present results suggest the feasibility of in-house cultivation of selected microalgae species as a quality live feed source for fish hatcheries.

Development of Hydrophobically Modified Casein Derivative-Based Delivery System for Docosahexaenoic Acids by an Acid-Induced Gelation.

Although omega-3 fatty acids including docosahexaenoic acid (DHA) contain various health-promoting effects, their poor aqueous solubility and stability make them difficult to be induced in dairy foods. The aims of this research were to manufacture casein derivative-based delivery system using acid-induced gelation method with glucono-σ-lactone and to investigate the effects of production variables, such as pH and charged amount of linoleic acid, on the physicochemical properties of delivery systems and oxidative stability of DHA during storage in model milk. Covalent modification with linoleic acid resulted in the production of casein derivatives with varying degrees of modification. As pH was reduced from 5.0 to 4.8 and the charged amount of linoleic acid was increased from 0% to 30%, an increase in particle size of casein derivative-based delivery systems was observed. The encapsulation efficiency of DHA was increased with decreased pH and increased charged amount of linoleic acid. The use of delivery system for DHA resulted in a decrease in the development of primary and secondary oxidation products. An increase in the degree of modification of casein derivatives with linoleic acid resulted in a decrease in the formation of primary and secondary oxidation products than of free DHA indicating that delivery systems could enhance the oxidative stability of DHA during storage in model milk. In conclusions, casein derivatives can be an effective delivery system for DHA and charged amount of linoleic acid played a key role determining the physicochemical characteristics of delivery system and oxidative stability of DHA.

Evaluation of genotype-environment interactions for non-polar lipids and fatty acids in chickpea (Cicer arietinum L.) seeds

Genotype-environment (G × E) interactions for non-polar lipids and fatty acids were studied in 28 chickpea accessions. The total nonpolar lipid content was determined by Soxhlet procedure; fatty acid profiles were investigated by gas chromatography. There were strong negative correlations between oleic and linoleic acids and between oleic and linolenic acids. The correlation between linoleic and linolenic acids was positive and either strong or moderate. Correlations between the other acids were differently directed and of various strengths. Line Luh 99/11 turned out to be an outlier in relation to the other genotypes due to an unusually high content of stearic acid. Cultivar CDC Jade was an outlier because of too low content of stearic acid and too high content of linoleic acid. Accession UD0502195 was an outlier due to a higher content of palmitic acid. Accessions UD0500022 and UD0502195 were outliers due to the low content of total nonpolar lipids. The variability in the total nonpolar lipid content was not affected by the environment, but the environment contributions to the variability of oleic and linoleic acids were very high. There were only statistically significant differences in the oleic and linoleic acid amounts between the cultivation years. There was a positive correlation between the oleic acid content and the average air temperature during the “anthesis – maturity” period and a negative correlation between the linoleic acid content and the average temperature during this period. There was also a negative correlation between the oleic acid content and precipitation during the “anthesis – maturity” period and a positive correlation between the linoleic acid content and precipitation during this period. The palmitic acid content was the most responsive to environmental changes in cultivar CDC Vanguard and the most resistant in cultivar Krasnokutskiy 123. The stearic acid content was the most sensitive to environmental changes in cultivar ILC 3279 and the most irresponsive in accession UKR001:0502116. As to oleic and linoleic acids, line L 273-18 had the bi (plasticity) and S2di (stability) values coupled with the corresponding mean contents, meaning that this genotype may be adapted to decreased temperature. The ecovalence values (Wi2) for the total nonpolar lipids, palmitic, stearic and linolenic acids indicated that these characteristics were little responsive to environmental fluctuations. As to oleic and linoleic acids, Wi2 values were much higher in many accessions, confirming the variability of these parameters depending on growing conditions. Having the highest Wi2 values, accession Garbanzo 2 is expected to show high degrees of the G × E interactions for oleic and linoleic acids. S2di was positively correlated with Wi2.

Comparative Study of Chemical Characteristic Analysis of Different Solvent-Extracted Solanum lycopersicum (Tomato Seed) Oil.

Tomato seeds are large waste byproducts from the processing of tomato into various food products like juice, sauce, and puree. One of the most potential uses of these tomato seeds is as a source of edible vegetable oil. The present study investigated the chemical characteristics including antioxidant activity, oxidative stability, and fatty acid composition of tomato seed extracted oil by aqueous and nonpolar (hexane) solvent. Fatty acid composition of tomato seed oils was determined by gas chromatography. Tomato seed oils were found to contain huge amount of linoleic acid, followed by oleic acid, palmitic acid, stearic acid, and linolenic acid. Polyunsaturated fatty acid and monounsaturated fatty acid content of the oil is near about 58% and 24%, respectively. Oxidative stability test of oil samples were evaluated by acid value, peroxide value, anisidine test, and TBA value. Antioxidant activity based on DPPH, FRAP, and ABTS assay of tomato seed oils was analyzed. The oil showed higher antioxidant activity-DPPH value (72-75%), FRAP value (9-11 µ mol/ml), and ABTS (37-39%). The results indicate hardly any difference in the fatty acid composition and antioxidant activity between aqueous and extracted and solvent-extracted tomato seed oil.