Amount Of Input Research Articles

Energy Budgeting of Different Cropping Sequences in the Indian Upper Gangetic Plains

Background: This study is meant to examine the energy requirement and energy input- output of different cropping sequences .The crucial objective to conduct experiment to understand energy efficiency using inputs and outputs which were disbursed in cropping sequences. Hence, it is need of the hour to identify the most remunerative and cost effective cropping sequence with high energy efficient for UGP of India. Methods: This study was carried out at the research farm of ICAR-Indian Institute of Farming Systems Research, Modipuram, during 2017-2021.The divergent cropping sequences viz. sugarcane-ratoon-wheat (CS1); rice-wheat-dhaincha (CS2); pigeonpea +maize-chickpea-okra (CS3); maize-berseem-black gram (CS4); sorghum-mustard-green gram (CS5) and Napier+cowpea/berseem (CS6) were compared in reference to curtail higher energy inputs through selected alternate cropping sequences. The obtained energy values were calculated by multiplying the amount of inputs and outputs by using energy conversion factors. Result: Maximum inputs energy consumed by sugarcane crop alone (33.14´103 MJ ha-1). Results shown that irrigation, seed, fertilizers and diesel required higher energy for the completion of cultural operations. However, higher inputs energy was used in irrigation followed by seed and fertilizers, respectively. In regard to per cent energy intake through inputs, the highest energy spent was for irrigation (35.30 MJ ha-1) and fertilizer (23.80 MJ ha-1).The wheat equivalent yield was higher in sugarcane-ratoon-wheat (125.58 t ha-1). Maximum output energy was with above system (596.70×103 MJ ha-1). Highest net energy returns was counted with sugarcane-ratoon-wheat (549.37´103 MJ ha-1), energy ratio (12.60) and energy profitability (11.60). Indeed, energy efficiency was highest in same system (1657.50) followed by maize-berseem-black gram (1421.96).

Establishment and Application of a New Radiation Biodosimetric Method Based on the Quantitative RPA-SHERLOCK Amplification Technology.

Biodosimetry is a key diagnostic tool for radiation exposure, risk assessment and treatment planning of acute radiation sickness. To effectively respond to a large-scale radiological incident, there is a need for the development of biodosimetric methods with fast, portable, and convenient operating advantages. We employed the recombinase polymerase amplification specific high-sensitivity enzymatic reporter unlocking (RPA-SHERLOCK) technology to establish a method for fast radiation dose assessment by measuring the expression level of radiation-inducible genes. Moreover, we proposed for the first time the principle of quantitative detection of curve slopes based on this method. Using this new method, changes in mRNA expression were confirmed in a number of radiation-sensitive genes (XPC, CDKN1A, and ATM) in human lymphocytes after irradiation. The standard curve of the dose-effect relationship was established, which can be used to quickly determine the exposed dose of the irradiated samples. Compared with traditional detection methods such as RT-qPCR, this method was found to be more convenient, fast and easy to operate. With the same amount of template input as RT-qPCR, the detection time of this method can be shortened to less than 20 min. The detection instrument required by this method is also more portable than a qPCR system.

What role does the environment play in language development? Exploring the associations among socioeconomic status, parent language input, and language skills in school-aged children with autism.

Language development in children with autism is influenced by proximal (e.g., parent language input) and distal (e.g., socioeconomic status) environmental constructs. Studies have found that "rich and responsive" parent language input supports autistic children's language development, and recent work has reported positive associations between measures of socioeconomic status (SES) and child language skills. However, little is known about how these proximal and distal environmental constructs interact to shape language development in autism. In a sample of 74 autistic school-aged children, the present study investigated the associations among measures of SES, the quantity and quality of language produced by parents and children during home-based dyadic parent-child interactions, and children's expressive and receptive language skills. Results showed that annual household income was positively associated with parent number of total words (NTW), parent number of different words (NDW), and parent mean length of utterance (MLU), while neither parent education level nor annual household income were significantly associated with measures of child language skills. Parent MLU was positively associated with child MLU and child expressive language skills. Findings suggest that annual household income may influence both the quantity and quality of parent language input, and that parent MLU, a qualitative measure of parent language input, may play a particularly important role in shaping autistic children's expressive language development. Future research should study longitudinal associations among SES, parent language input, and child language skills, as identifying environmental predictors of language skills in autism may facilitate the creation of more effective interventions that support language development.

The Effect of Book Genre in Eliciting Teacher Math Talk

ABSTRACTNumber books are an effective tool to increase math talk and promote children's mathematical thinking, but little is known about book genre effects. How do different types of number books impact mathematical input? This study examined whether book genre affects the amount and types of teachers' math talk. Using a within‐subjects design, we asked 31 teachers of 4‐ to 5‐year‐olds to read a narrative number book and a nonnarrative number book in a counterbalanced order. The books were matched in target numerical content. We found that the nonnarrative number book elicited more math talk than the narrative number book. In a qualitative analysis, we analyzed multiple conversational turns and found that both books allowed teachers to engage in simple math discussions, but there were differences in other types of math discussions. Together, these findings show that the quantity and quality of mathematical input differ as a function of genre.

In vitro ruminant fermentation, degradation, and methane production of four agro-industrial protein-rich co-products, compared with soyabean meal

Soyabean is considered an unsustainable protein source for livestock feeds because of the large quantity of input and energy required to cultivate and process it. Other protein-based agro-industrial co-products that are less input-intensive, can mitigate methane (CH4) production and may therefore be more sustainable options instead soyabean. The objective of this study was to compare the effect of replacing the same amount of protein (40g/kg DM crude protein) as soyabean meal (SBM) with low-carbon local agro-industrial co-products, (brewers’ spent grains, BSG; dried wheat distillers’ grains, WDG; dried corn distillers’ grains, CDG and corn steep liquor CSL), on in vitro rumen degradation, fermentation and gas and methane production. The study used a 72-hour in vitro gas production method with a basal substrate of dried, ground grass silage and wheat. Gas volumes were measured at eleven different specific intervals, and CH4 concentrations were analysed via gas chromatography. After 72hours, in vitro DM degradability (IVDMD) and volatile fatty acid (VFA) concentrations were assessed. Gas and CH4 production curve profiles were fitted to models to determine asymptote production, the extent of degradation in rumen proper (RoP), and the fractional degradation rate (μ) (h-1) in the halfway 50% of the asymptote production. The IVDMD and estimated RoP at 0.04h and 0.025h were lower (P<0.05) for BSG compared to the other treatments, by 4.9 – 6.6%; 5.8 - 9.9%; 5.2 - 9.0%. Gas and CH4 yield (mL/g substrate and mL/g substrate degraded), and pH (SB = 6.77, BSG = 6.80, WDG = 6.74, CDG = 6.84, and CSL = 6.73; P>0.05), were not significantly affected by treatment. Butyrate and valerate were lower (P<0.05) for BSG compared to CSL, and caproate was lower (P<0.001) for BSG compared to the other treatments and in CSL compared to SBM. The results regarding degradability and VFAs concentrations of this study demonstrated that dried wheat distillers’ grains, dried corn distillers’ grains, and corn steep liquor have the potential to replace soyabean meal as protein sources for ruminants, but further reduction of CH4 emissions as a result of such practice may not be expected. Although slightly less degradable, based on their nutrient composition and the fact they did not affect rumen fermentation characteristics, brewers' spent grains can still play a complementary role in ruminant diets, especially in regions where they are locally readily available.

Geometric optimization to improve the performance of a new design of concentration‐based solar distiller using particle swarm optimization algorithm

AbstractSolar distillers are one of the oldest and simplest technologies for desalinating salt water using solar energy. The main problem is the low freshwater production compared to the amount of energy input from the sun. To overcome this problem, a solar distiller was developed using a parabolic trough concentrator to increase the freshwater yield. The geometry optimization of various parameters of the developed system was also studied using the particle swarm optimization (PSO) algorithm to obtain the optimal design and give the improved freshwater yield. The simulation is carried out based on the algorithm using the climatic condition data of Rabat City, Morocco. Numerical resolution of the energy balance equations was performed using MATLAB code. In terms of flexibility, speed, and global convergence, this method's final results were satisfactory. The maximum optimized freshwater yield using the PSO approach is 12.83 kg/m2 and the maximum optimized energy and exergy efficiency were about 204.7% and 34.3%, respectively. Furthermore, the results showed that the absorber basin accounts for the highest exergy destruction, 88.7% of the total optimized exergy destruction was found from exergy analysis.

Alunite in Cross Crater, Mars: Evidence for a Possible Site of Ancient Life.

Cross Crater is a 65-km impact crater located in the Noachian highlands of the Terra Sirenum region of Mars. Geochemical modeling has indicated that alunite detected on the southwest wall of Cross Crater could have been formed by a fumarole upwelling into Cross Crater Lake and could indicate that an environment favorable to the development of life may have existed several billion years ago. Alunite did not form when Noachian precipitation reacted with basalt nor when the sediments and groundwater resulting from this reaction were reacted with a fumarole. Only when Cross Crater Lake water was equilibrated with sulfuric acid, thought to be a major component of the atmosphere in the Hesperian, following reaction with fumarole groundwater, did alunite precipitate from solution. Kaolinite, silica, or an Al-smectite such as montmorillonite also formed. The proximity of Cross Crater to the Tharsis volcanic region relative to Columbus crater, where alunite has also been detected, may have resulted in larger amounts of magmatic water input to the lake from sources along fractures that extend westward from Tharsis. This could explain the more extensive deposit of alunite at Cross Crater relative to Columbus crater.

Risk Assessment of Heavy Metal Accumulation in Cucumber Fruits and Soil in a Greenhouse System with Long-Term Application of Organic Fertilizer and Chemical Fertilizer

Combining organic and chemical fertilizers is a sustainable strategy for vegetable production. However, there is limited research concerning the risks associated with heavy metals (HMs) in greenhouse systems with long-term location application. A three-year investigation, conducted from 2021 to 2023, explored a fifteen-year field experiment with combinations of chemical fertilizer (CH), corn straw (SW) and pig manure (PM). Five treatments were evaluated: excessive fertilization (high CH and PM), conventional fertilization (normal CH), organic–inorganic fertilization (3/4CN + 1/4PN, 2/4CN + 2/4PN and 2/4CN + 1/4PN + 1/4SN). This study evaluated the risks associated with heavy metals (HMs) by analyzing and quantifying their concentrations in soil and cucumber fruits, as well as by calculating the bioconcentration factors (BCFs), the geo-accumulation index (Igeo), and both the non-carcinogenic and carcinogenic risks. The results indicated that excessive fertilization (CF) increased the concentrations of Cu and Zn in fruits, as well as the Igeo values of Cu, Zn, and Cd, and the non-carcinogenic Cu risk, while decreasing the BCFs of Cu and Zn. Organic–inorganic fertilization also elevated the Igeo values of Cu and Zn. Redundancy analyses confirmed a positive correlation between the soil concentrations of Cu and Zn and higher levels of available phosphorus contents (48.4%), alongside a lower pH (4.9%). The concentrations of Cu, Zn, and Cd in both soil and cucumber fruits increased linearly with the duration of application and amount of input. Although the combined application of CH with PM or SW did not significantly elevate the non-carcinogenic or carcinogenic risks associated with most heavy metals, the carcinogenic risks of Cd and As emerged as potential risk factors after 15 years of organic–inorganic fertilization. Utilizing a combination of CH with PM and SW as a fertilizer management strategy can effectively address both the control of heavy metal inputs in the facility and the safety and quality of cucumbers.

Developing automaticity in neural speech discrimination in typically developing bilingual Italian-German and monolingual German children.

Many studies have shown that input in more than one language influences children's phonemic development. In this study, we examined the neural processes supporting perception of Voice Onset Time (VOT) in bilingual Italian-German children and their monolingual German peers. While German contrasts short-lag and long-lag, Italian contrasts short-lag and voicing lead. We examined whether bilinguals' phonetic/phonological systems for the two languages develop independently or whether they influence each other, and what role language input plays in the formation of phonetic/phonological categories. Forty five-year-old children (16 monolingual German, 24 bilingual Italian-German) were tested in an oddball design expected to elicit a neural Mismatch Response (MMR). The stimuli were bilabial stop VOT contrasts with the short-lag stop, common to both languages, as the standard. Four deviant VOTs were selected: 92 ms and 36 ms lag for German; 112 ms and 36 ms voicing lead for Italian. Bilingual children's language background was assessed using a caregiver questionnaire. Italian-German bilingual 5-year-old children and German monolingual controls showed similar MMRs to German long-lag and Italian voicing lead VOT, except for the 36 ms long-lag deviant; this acoustically difficult distinction did not elicit a robust negative MMR in the bilingual children. The lack of a difference between the bilinguals and monolinguals for voicing lead suggests that the amount of input in Italian for the bilinguals was not sufficient to lead to an advantage compared to the monolingual German children. Alternatively, the finding could indicate that voicing lead is easier to discriminate than voicing lag.

Economic impact of inclusion of black soldier fly products in broiler diets: A comparison between conventional and higher animal welfare production systems in the Netherlands

The primary objectives of this study were to analyze economic feasibility of incorporating black soldier fly products in broiler feed and to compare the viability of alive (alive grown larvae (AGL)) or processed (defatted larvae meal (DLM) and insect oil) insect inclusion into the two Dutch broiler welfare systems: conventional and better life one star (BLS).An existing simulation model for insect production was adapted to build an economic model for the insect-fed broiler breast meat supply chain. Amounts, full costs, and breakeven prices of different production setups were compared and the impacts of specific input parameters were tested by sensitivity and breakeven analyses.Results indicated a hypothetical demand of 2.15 million tons wet matter of raw substrate to meet the total Dutch breast meat production (BLS broilers and 10% AGL inclusion). In case of 10% processed insect inclusion, the conventional broiler breast meat price increased from 3,947 to 6,069 €/t (53.7%) and the BLS breast meat price from 6,493 to 9,662 €/t (48.8%). Alive insect inclusion resulted in a smaller cost increase (42.2%) by skipping the costly processing step. To become price competitive, DLM or AGL must cost 618 or 801 €/ton dry matter (tDM), respectively, requiring a raw substrate mix price of −109 €/tDM.High input amounts and costs currently hinder the widespread adoption of insect products in Dutch mass poultry feed. However, in the long run, using negative value substrates or targeting niche markets for broiler welfare with low AGL inclusion levels could become viable business models.Research is needed on the impact of negative value substrates on insect production performance and contamination risks. Furthermore, the impact of varying AGL composition requires special attention.

Independent effects of tree diversity on aboveground and soil carbon pools after six years of experimental afforestation.

Planting diverse forests has been proposed as a means to increase long-term carbon (C) sequestration while providing many co-benefits. Positive tree diversity-productivity relationships are well established, suggesting more diverse forests will lead to greater aboveground C sequestration. However, the effects of tree diversity on belowground C storage have the potential to either complement or offset aboveground gains, especially during early stages of afforestation when potential exists for large losses in soil C due to soil decomposition. Thus, experimental tests of the effects of planted tree biodiversity on changes in whole-ecosystem C balance are needed. Here, we present changes in above- and belowground C pools 6 years after the initiation of the Forests and Biodiversity experiment (FAB1), consisting of high-density plots of one, two, five, or 12 tree species planted in a common garden. The trees included a diverse range of native species, including both needle-leaf conifer and broadleaf angiosperm species, and both ectomycorrhizal and arbuscular mycorrhizal species. We quantified the effects of species richness, phylogenetic diversity, and functional diversity on aboveground woody C, as well as on mineral soil C accumulation, fine root C, and soil aggregation. Surprisingly, changes in aboveground woody C pools were uncorrelated to changes in mineral soil C pools, suggesting that variation in soil C accumulation was not driven by the quantity of plant litter inputs. Aboveground woody C accumulation was strongly driven by species and functional identity; however, plots with higher species richness and functional diversity accumulated more C in aboveground wood than expected based on monocultures. We also found weak but significant effects of tree species richness, identity, and mycorrhizal type on soil C accumulation. To assess the role of the microbial community in mediating these effects, we further compared changes in soil C pools to phospholipid fatty acid (PLFA) profiles. Soil C pools and accumulation were more strongly correlated with specific microbial clades than with total microbial biomass or plant diversity. Our results highlight rapidly emerging and microbially mediated effects of tree biodiversity on soil C storage in the early years of afforestation that are independent of gains in aboveground woody biomass.

Effect of Microplastics and Straw Addition on Nitrogen and Phosphorus Leaching in Orange Orchard Soils

To explore the effects of microplastic input and straw addition on nitrogen and phosphorus leaching in orange orchard soil, indoor soil column leaching simulation experiments were conducted on orange orchard soil in Dangyang City, Hubei Province. The experiment analyzed the effects of different treatments on the leaching characteristics of soil nitrogen and phosphorus. The results showed that： ① The increase in microplastic input increased the leaching capacity of the soil （TN, NO3--N, NH4+-N, and TP） when only microplastic input was added. ② Under the addition of straw, the input of microplastics increased, which reduced the leaching amount of TN and NO3--N in the soil, and increased the leaching amount of NH4+-N and TP in the soil. ③ Under the input of microplastics, the key influencing factors of soil nitrogen and phosphorus leaching were soil bulk density and water content. Under straw addition, it was mainly affected by soil carbon and nitrogen content. Among them, microplastic input and straw addition significantly increased soil pH, but their path coefficients for nitrogen and phosphorus leaching were not significantly correlated. The results showed that the effects of polypropylene microplastic input and straw addition on soil nitrogen and phosphorus leaching were related to the amount of microplastics input and whether straw was added. The results showed that the input of microplastics would increase the amount of soil nitrogen and phosphorus leaching. Although nitrogen leaching loss caused by microplastic input could be reduced under straw addition, soil phosphorus leaching loss was significantly increased.

Mapping the pathway and support offered to children with an intellectual disability referred to specialist mental health services in the UK.

This survey of 66 specialist mental health services aimed to provide an up-to-date description of pathways of care and interventions available to children with an intellectual disability referred for behaviours that challenge or with suspected mental health problems. Overall, 24% of services made contact with a family at referral stage, whereas 29% contacted families at least once during the waiting list phase. Only two in ten services offered any therapeutic input during the referral or waiting list stages. During the active caseload phase, services offered mostly psychoeducation (52-59%), followed by applied behaviour analytic approaches for behaviours that challenge (52%) and cognitive-behavioural therapy (41%). Thirty-six per cent of services had not offered any packaged or named intervention in the past 12 months. With increasing waiting times for specialist mental health support, services need to consider increasing the amount of contact and therapeutic input on offer throughout all stages of a child's journey with the service.

Organic geochemistry and 1D-basin modeling in the Taranaki Basin, New Zealand: Implications for deltaic-source rocks of the cenozoic oil and condensate reservoirs

The Taranaki Basin in New Zealand is an area of active exploration for oil and condensate. This research focuses on integrating geochemical characteristics and 1-D basin modeling to Late Cretaceous to Miocene source rock systems, along with oil and condensate data from fifty-seven wells in the Taranaki Basin. The geochemical study reveals that the oil and condensate samples were generated from clay-rich source rocks, containing mixed organic matter, with large amounts of terrestrial organic matter input. These source rocks were deposited in fluvial to fluvio-deltaic environments under oxic conditions. The presence of oleanane in both oil and condensate samples suggests that the source rocks had a significant terrestrial component and deposited during the Late Cretaceous to Cenozoic. Using various biomarker proxies, oil-source rock correlation along with 1-D basin modeling revealed that the oil and condensate were mainly derived from the Late Cretaceous Rakopi and Paleocene Farewell formations at different maturity stages. The oils were generated within the peak-mature oil window, while the condensates primarily resulted from the secondary cracking of oil taking place in the source rock within the gas generation window. This finding is consistent with the 1-D basin modeling results. The model shows that the Paleocene Farewell source rock has achieved the primary stage of oil generation (0.55–0.95 Easy %Ro), contributing to most of the discovered oils in the Cenozoic reservoir rocks. Meanwhile, the Late Cretaceous Rakopi source rock reached the gas window with a higher vitrinite reflectance of more than 1.30 Easy %Ro, indicating greater gas generation potential.

Energy Consumption in Wheat-Rice Cropping System in Punjab

Energy consumption for crop production depends on the availability of energy sources and the capacity of the farmers. This study analyzed energy sources used by farmers for wheat and rice production and identified agricultural operations where energy consumption can be reduced so that operational costs in wheat-rice production system can be lowered. Data on energy use in wheat and rice cropping system was collected by interviewing the farmers using specially designed questionnaire. Relevant information pertaining to human labour use, sources of irrigation, number of irrigations to each crop, quantity of farm inputs, utilization of mechanical power sources and grain yield etc. were collected. The average output energy of grain, input energy, energy ratio and specific energy for wheat crop in the state of Punjab were 68347 MJ ha-1, 15644 MJ ha-1, 8.4 and 3.08 MJ kg-1, respectively. The average output energy of grain, input energy, energy ratio and specific energy for rice were 115888 MJ ha-1, 16438 MJ ha-1, 14.8 and 2.09 MJ kg-1, respectively. For wheat-rice cropping system, the average output energy of grain, input energy, energy ratio and specific energy were 184234.4 MJ ha-1, 32081.4 MJ ha-1, 11.7 and 2.48 MJ kg-1, respectively. Energy ratio (output energy to input energy ratio) of rice is higher than the wheat, indicating rice efficiently utilized energy resources to convert into output of the harvested crop. Similarly, lower specific energy (input energy to yield ratio) of rice as compared to wheat also indicates lower energy consumption to produce per unit of rice. This analysis clearly indicates that rice production is more energy efficient than wheat production in rice-wheat production system. Further, the energy ratio, specific energy and energy productivity of wheat-rice cropping system were estimated as 11.6 to 11.9, 2.44 to 2.51 MJ kg-1 and 0.40 to 0.41kg MJ-1, respectively. This study will be helpful to identify the agricultural operations and implementing suitable interventions for improving energy use efficiency.

Whole-brain annotation and multi-connectome cell typing of Drosophila

The fruit fly Drosophila melanogaster has emerged as a key model organism in neuroscience, in large part due to the concentration of collaboratively generated molecular, genetic and digital resources available for it. Here we complement the approximately 140,000 neuron FlyWire whole-brain connectome1 with a systematic and hierarchical annotation of neuronal classes, cell types and developmental units (hemilineages). Of 8,453 annotated cell types, 3,643 were previously proposed in the partial hemibrain connectome2, and 4,581 are new types, mostly from brain regions outside the hemibrain subvolume. Although nearly all hemibrain neurons could be matched morphologically in FlyWire, about one-third of cell types proposed for the hemibrain could not be reliably reidentified. We therefore propose a new definition of cell type as groups of cells that are each quantitatively more similar to cells in a different brain than to any other cell in the same brain, and we validate this definition through joint analysis of FlyWire and hemibrain connectomes. Further analysis defined simple heuristics for the reliability of connections between brains, revealed broad stereotypy and occasional variability in neuron count and connectivity, and provided evidence for functional homeostasis in the mushroom body through adjustments of the absolute amount of excitatory input while maintaining the excitation/inhibition ratio. Our work defines a consensus cell type atlas for the fly brain and provides both an intellectual framework and open-source toolchain for brain-scale comparative connectomics.

B-231 Evaluation of the KingFisher Flex for DNA Isolation From FFPE Tissue Utilizing Autolys M Tubes and the MagMAX FFPE DNA/RNA Ultra Kit

Abstract Background Our institution is facing increasing demand for oncology testing that requires high quality DNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue. The current DNA extraction workflow involves a time and labor-intensive manual process that limits our ability to accommodate increasing downstream clinical testing volumes. We evaluated the KingFisher (KF) Flex utilizing Autolys M Tubes and the MagMAX FFPE DNA/RNA Ultra Kit as a possible high-throughput, automated DNA isolation workflow option that would decrease hands-on time and be conducive to volume growth without the need for additional staff. Methods DNA from 52 FFPE tissue samples (n=24 unique cases previously extracted by the current “manual method”) were extracted using Autolys M tubes, the MagMAX FFPE DNA/RNA Ultra Kit, and the KF Flex (Thermo Fisher Scientific Waltham, MA) over 4 “automated method” runs. Method modifications to the published procedure were introduced over the runs to determine any effects on DNA quality/quantity. Timings were taken during each run to compare hands-on and total run time against that of the manual method. Precision experiments were performed by extracting 4 replicates of sample on a single run (intra-assay precision) and 2 samples from 2 cases over 2 runs (inter-assay precision). Three cases were processed using varying tissue input levels to assess input-yield correlation. DNA yield was measured using HS dsDNA Qubit (Thermo Fisher Scientific, Waltham, MA). A subset of samples was tested by digital droplet PCR (ddPCR) (n=10), chromosomal microsomal microarray (CMA) (n=1), and next-generation sequencing (NGS) (n=3). Variants and QC metrics of the KF DNA were compared to those of the manual method DNA. Results The average total DNA yield was 3% lower from samples extracted following the manufacturer recommended KF procedure compared to matched samples extracted using the manual method (n=28 samples from 23 unique cases). A CV of 2.8% was observed in an intra-assay experiment (n=4 replicates from 1 case) and 3.0% in an average inter-assay experiment (n=2 replicates from 2 cases). There was a direct correlation between the tissue input amount (4 amounts of varied tissue input) and the total DNA yield (n=3 samples). We observed concordant fractional abundance values for ddPCR samples and concordant variant detection for NGS and CMA samples. We did not see a significant difference in the quality metrics tracked for any of the downstream testing assays. Conclusions This evaluation demonstrated that, as compared to our manual method, the automated method resulted in comparable DNA yields and downstream assay results. The incorporation of this automation would significantly increase our FFPE DNA extraction capacity without requiring additional staff while reducing hands-on time per run by about six hours. We would also be able to reduce ergonomic hazards related to tube cap manipulation, eliminate use of hazardous reagents, and leverage the plate format to better integrate extracted DNA into our clinical sample preparation workflows. These operational benefits highlight the value of automating high-throughput workflows.

조선용 B Gr. 강의 용접부 미세조직과 기계적 성질에 미치는 용접기법의 영향

The microstructurale and mechanical properties of B Gr. steel were evaluated for shipbuilding according to a welding process. The following conclusions were drawn. In tandem submerged arc welding(TSAW), compared to single submerged arc welding(SAW), the grain boundary ferrite was coarsened, and the fraction was higher owing to the high heat input and low flux basicity ; conversely, the volume fraction of acicular ferrite was lower. Fine intragranular acicular ferrite and polygonal ferrite were observed in the weld metal of the electrogas welding (EGW), which had the highest heat input. In the microstructure near the fusion line, as the amount of heat input increased, the prior austenite grain boundary became coarse. Further, both the intergranular grainboundaryferrite and intragranular bainite became coarse, and the heat-affected zone became wider. The hardness of weld metal was independent of the amount of heat input and was demonstrated to be high in the order of EGW, single SAW, and TSAW owing to the microstructure difference caused by the chemical composition of the filler material and the dilution rate of the base metal. Furthermore, the tensile strength of all three welding processes resulted in stable satisfaction with the class society rules.

The role of individual differences in L2 vocabulary learning: A review of out-of-class exposure, strategic learning and motivation

This review paper explores the role of individual differences in second language vocabulary learning, focusing on three key factors: out-of-class exposure (e.g., viewing TV, playing video games and listening to songs), strategic vocabulary learning, and motivation. Individual differences significantly impact vocabulary learning, making it crucial to understand how these factors contribute to learning outcomes. Yet, previous reviews have mainly overlooked out-of-class exposure in their discussion of individual differences. The current review shows that the three factors can have a positive effect on lexical development. There are some points to consider such as the need for a large amount of input for out of-class exposure to be effective. Additionally, the review shows that both motivation and self-regulation are important for vocabulary learning and that they are positively related to vocabulary knowledge. It shows that more self-regulated and motivated students tend to have significantly larger and more developed vocabulary knowledge. By synthesizing findings from empirical studies on individual differences and vocabulary learning, this review provides insights into making vocabulary learning more effective.

The role of individual differences in L2 vocabulary learning: A review of out-of-class exposure, strategic learning and motivation

This review paper explores the role of individual differences in second language vocabulary learning, focusing on three key factors: out-of-class exposure (e.g., viewing TV, playing video games and listening to songs), strategic vocabulary learning, and motivation. Individual differences significantly impact vocabulary learning, making it crucial to understand how these factors contribute to learning outcomes. Yet, previous reviews have mainly overlooked out-of-class exposure in their discussion of individual differences. The current review shows that the three factors can have a positive effect on lexical development. There are some points to consider such as the need for a large amount of input for outof-class exposure to be effective. Additionally, the review shows that both motivation and self-regulation are important for vocabulary learning and that they are positively related to vocabulary knowledge. It shows that more self-regulated and motivated students tend to have significantly larger and more developed vocabulary knowledge. By synthesizing findings from empirical studies on individual differences and vocabulary learning, this review provides insights into making vocabulary learning more effective.