Amount Of Geosmin Research Articles

Geosmin production and polyphasic characterization of Oscillatoria limosa Agardh ex Gomont isolated from the open canal of a large drinking water system in Tianjin City, China

Taste and odor (T & O) episodes always cause strong effects on drinking water supply system. Luanhe River diversion into Tianjin City in China is an important drinking water resource. Massive growth of a benthic filamentous cyanobacterium with geosmin production in the open canal caused a strong earthy odor episode in Tianjin. On the basis of the morphological and molecular identification of this cyanobacterium as Oscillatoria limosa Agardh ex Gomont, the genetic basis for geosmin biosynthesis and factors influencing growth and geosmin production of O. limosa CHAB 7000 were studied in this work. A 2268-bp open reading frame, encoding 755 amino acids, was amplified and characterized as the geosmin synthase gene (geo), followed by a cyclic nucleotide-binding protein gene (cnb). Phylogenetic analysis implied that the evolution of the geosmin genes in O. limosa CHAB 7000 might involve a horizontal gene transfer event. Examination on the growth and geosmin production of O. limosa CHAB 7000 at different light intensities showed that the maximum geosmin production was observed at 10μmol photons m−2s−1, while the optimum growth was at 60μmol photons m−2s−1. Under three temperature conditions (15°C, 25°C, and 35°C), the maximum growth and geosmin production were observed at 25°C. Most amounts of geosmin were retained in cells during the growth phase, but high temperature and low light intensity increased the release of geosmin into the medium, implying that O. limosa CHAB 7000 had a high potential harm for the release of geosmin from its cells at these adverse conditions.

Freundlich adsorption isotherms of agricultural by-product-based powdered activated carbons in a geosmin-water system.

The present study was designed to model the adsorption of geosmin from water under laboratory conditions using the Freundlich isotherm model. This model was used to compare the efficiency of sugarcane bagasse and pecan shell-based powdered activated carbon to the efficiency of a coal-based commercial activated carbon (Calgon Filtrasorb 400). When data were generated from Freundlich isotherms, Calgon Filtrasorb 400 had greater geosmin adsorption at all geosmin concentrations studied than the laboratory produced steam-activated pecan shell carbon, steam-activated bagasse carbon, and the CO2-activated pecan shell carbon. At geosmin concentrations < 0.07 microg/l for the phosphoric acid-activated pecan shell carbon and below 0.08 microg/l for a commercially produced steam-activated pecan shell carbon obtained from Scientific Carbons, these two carbons had a higher calculated geosmin adsorption than Filtrasorb 400. While the commercial carbon was more efficient than some laboratory prepared carbons at most geosmin concentrations, the results indicate that when the amount of geosmin was below the threshold level of human taste (about 0.10 microg/l), the phosphoric acid-activated pecan shell carbon and the Scientific Carbons sample were more efficient than Filtrasorb 400 at geosmin removal.

Release of geosmin by Anabaena circinalis following treatment with aluminium sulphate

Taste and odour due to algae are not generally removed during conventional water treatment and can sometimes be increased during the treatment process owing to cell lysis. Some reasons for this have been documented in the literature but there appears to be contradictory evidence regarding the effect of flocculation. In this study the effect of aluminium sulphate on cultured cells of Anabaena circinalis was evaluated at concentrations and conditions that would occur in a water treatment plant. Aluminium sulphate did not appear to cause lysis of cells of cultured Anabaena circinalis nor increase the amount of geosmin in the water. Flocculation under laboratory conditions, which simulated operating water treatment plants, resulted in removal of cells in a healthy state, with no additional release of geosmin.

Variation of geosmin content in Anabaena cells and its relation to nitrogen utilization

The addition of the proper amount of ammonium to the culture medium containing nitrate as nitrogen source enhanced the growth rate of Anabaena viguieri. The amount of geosmin produced by these cells varied with the concentrations of ammonium added. A negative correlation between the amount of geosmin produced and of the growth rate of cells was revealed. This was also found in cells grown on various forms of nitrogen sources. Without supply of any nitrogen compound, this organism is capable of fixing gaseous nitrogen, and under these conditions the cells grew relatively slowly. However, they produced more geosmin (per unit protein mass) than cells grown in the presence of combined nitrogen. The isolation of heterocysts, in which nitrogen was fixed, showed that these cells produced higher amounts of geosmin than vegetative cells. The possible relation of nitrogen assimilation to the production of geosmin in the cells was discussed.