Amounts Of Fructooligosaccharides Research Articles

Dietary Supplementation of Fructooligosaccharides Enhanced Antioxidant Activity and Cellular Immune Response in Broiler Chickens.

This study investigated the impact of various concentrations of fructooligosaccharides (FOS) prebiotic on the production performance, antioxidant status, and immune response of broiler chicken. The FOS was used at 0, 0.3, 0.5, and 0.7%. The cycle included 340 broilers distributed into 4 batteries, with 85 broiler chickens in each battery. There were 5 replicates with 17 broiler chickens each, and the analyses were triplicated. The studied parameters were production performance, antioxidant status, hematological measurements, cellular and humoral immune response, intestinal acidosis, intestinal microbial counts, and volatile fatty acid (VFA) level in the hindgut. Results showed that broiler chickens fed 0.7% of FOS had significantly higher body weight gain than the control group and the groups fed 0.3% and 0.5% of FOS. Supplementing broiler feed with FOS at all levels increased the total antioxidant capacity (TAC) and reduced the malondialdehyde of the sera (P = 0.015 and 0.025, respectively). Liver catalase enzyme in the broiler chickens fed 0.5 and 0.7% of FOS was higher than that of the control group and the group fed 0.3% of FOS (P = 0.001). However, the liver MDA of the control group was higher than that of all the other groups (P = 0.031). The total WBC and heterophils % were the highest after supplementing broilers with 0.7% FOS (P = 0.004 and 0.003, respectively) at 3 wks of age. Conversely, lymphocytes and monocytes were the lowest for the 0.7% FOS group (P = 0.030 and 0.020, respectively). Dietary 0.05 and 0.7% of FOS induced the highest cellular response compared to the other treatments (P = 0.020). Thymus, bursa of Fabricious, and spleen weights were enhanced after FOS supplementation, which indicates a higher specific cellular response. To conclude, FOS prebiotic at all levels can be utilized safely to enhance the antioxidant activity and the cellular immune response of broiler chickens. Using 0.7% of FOS resulted in higher body weight of broilers. Accordingly, this amount of FOS is sufficient to reach the required results.

Acceptability of yacon flakes (Smallanthus sonchifolia) for dietary use with inulin

This study aimed to elaborate on yacon flakes and determine the proximal composition of fresh yacon and final flakes, made at different temperatures (120°C, 150°C and 180°C) for 20 mins. We measured the acceptability of the sensory attributes: taste, texture, odour and colour and the global acceptability at the laboratory level, using semi-trained panellists and an unstructured hedonic scale. The acceptability of the flakes was measured at different temperatures. Our results indicate that the flakes made at 180°C had the highest acceptance (87%), and the flakes made at 120°C had 80% of unacceptability. Our data were analysed according to the analysis of variance (ANOVA) and Tukey's test. We found significant differences between the averages of the taste scores (T0:7.1; T1:7.6; T2:6.7; T3:4.6), p<0.05; texture (T0:7.1; T1:7.2; T2:5.7; T3:4.5), p<0.05; and odour (T0:7.2; T1:7.6; T2:5.1; T3:3.3), p<0.05. The amount of fructooligosaccharides on a fresh basis was Ⱦ±SD (31.60±0.650) and on a dry basis (37.44±1DS). Microbiological analysis shows that the flakes are fit for human consumption. It is concluded that yacon flakes contain significant amounts of inulin, being a good substitute alternative for the preparation of snack-type dietetic products.

Development of a frozen drink based on prebiotic lactic beverage: physicochemical, microbiological and sensory aspects

The improper disposal of residual whey from dairy production represents a serious environmental problem in Brazil, causing a lot of effort applied in the development of dairy products with partial replacement of milk by whey. In this study, three formulations of a frozen dairy drink containing whey and different amounts of fructooligosaccharides, in replacing saccharose, were developed and evaluated in relation to physicochemical, microbiological and sensory properties. All physicochemical and microbiological parameters were within Brazilian regulations for frozen edibles. Evaluated by 120 volunteers, the Acceptability Index was above 80% for all formulations and attributes and the purchase intention favourable the acquisition of the product. The tested formulations represent a nutritious and safe alternative for the use of residual whey from dairy farms and an innovation on the market of functional frozen edibles.

Effects of inulin, fructooligosaccharides/glucose and pH on the shape of the death kinetic of Lactobacillus reuteriDSM 20016

SummaryThe viability of Lactobacillus reuteri, Lb. delbrueckii subsp. bulgaricus and Lb. paracasei was assessed in a laboratory medium containing inulin (5 g L−1) or glucose + FOS (fructooligosaccharides) (2.5 + 2.5 g L−1). Data were modelled through the Weibull equation pinpointing that prebiotics determined a decrease in the shape parameter. In the 2nd step, L. reuteri was used as the test microorganism; pH, storage temperature and the amounts of FOS + glucose and inulin were combined through a 4‐variable/5‐level central composite design. Temperature, pH and glucose + FOS affected the microbiological shelf life; inulin was not significant. The maximum value of shelf life was found at pH 8.5 and with 5.0 g L−1 of glucose + FOS. The combination of pH and prebiotics affected the shape parameter with a shift from a convex to a concave trend and vice versa: their effect was different depending on the use of a single prebiotic or a mix.

DYNAMICS OF ACCUMULATION AND PARTITIONING OF DRY MATTER AND FRUCTO-OLIGOSACCHARIDES IN PLANT FRACTIONS OF FORAGE CEREALS

SUMMARYDuring growth, several cereals store significant amounts of fructo-oligosaccharides (FOS), which have important prebiotic properties. Cereal forage crops are also essential components of many Mediterranean agricultural systems, although little information is available on their dynamics of accumulation and partitioning of dry matter and FOS during growth. Oat (Avena sativaL.,cv. ‘Flavia’ andcv. ‘Genziana’), emmer wheat (Triticum dicoccumSchrank,cv. ‘Giovanni Paolo’), barley (Hordeum vulgareL.,cv. ‘Diomede’) and triticale (xTriticosecaleWittmack,cv. ‘Rigel’) were investigated for their synthesis of FOS, with a view to development of management approaches for harvesting high-quality forage, and to determine whether these species can be used as natural sources of FOS for commercial use. The study was conducted at Foggia (Italy) and Bella (Potenza, Italy) over two growing seasons (2008–2009; 2009–2010). Dry-matter accumulation and FOS contents were determined for plant fractions from heading to kernel-hard stages. There were large variations across these species for dry-matter partitioning and dry-matter yield (greatest for triticale: 1.24 kg m−2), and for FOS levels of total plants and plant fractions. Emmer wheat and triticale showed greater FOS production (52.0, 41.1 g m−2, respectively). Barley, emmer wheat and triticale showed higher FOS levels in total plants (4.11%, 5.93%, 4.33% dry matter, respectively). Barley, emmer wheat and triticale appear to be the most interesting species for production of forage biomass rich in FOS and as natural FOS sources for industrial use.