American Association Of Endodontists Research Articles

Deep learning for determining the difficulty of endodontic treatment: a pilot study

BackgroundTo develop and validate a deep learning model for automated assessment of endodontic case difficulty from periapical radiographs.MethodsA dataset of 1,386 periapical radiographs was compiled from two clinical sites. Two dentists and two endodontists annotated the radiographs for difficulty using the “simple assessment” criteria from the American Association of Endodontists’ case difficulty assessment form in the Endocase application. A classification task labeled cases as “easy” or “hard”, while regression predicted overall difficulty scores. Convolutional neural networks (i.e. VGG16, ResNet18, ResNet50, ResNext50, and Inception v2) were used, with a baseline model trained via transfer learning from ImageNet weights. Other models was pre-trained using self-supervised contrastive learning (i.e. BYOL, SimCLR, MoCo, and DINO) on 20,295 unlabeled dental radiographs to learn representation without manual labels. Both models were evaluated using 10-fold cross-validation, with performance compared to seven human examiners (three general dentists and four endodontists) on a hold-out test set.ResultsThe baseline VGG16 model attained 87.62% accuracy in classifying difficulty. Self-supervised pretraining did not improve performance. Regression predicted scores with ± 3.21 score error. All models outperformed human raters, with poor inter-examiner reliability.ConclusionThis pilot study demonstrated the feasibility of automated endodontic difficulty assessment via deep learning models.

Comparative analysis of diagnostic accuracy in endodontic assessments: dental students vs. artificial intelligence.

This study evaluates the comparative diagnostic accuracy of dental students and artificial intelligence (AI), specifically a modified ChatGPT 4, in endodontic assessments related to pulpal and apical conditions. The findings are intended to offer insights into the potential role of AI in augmenting dental education. Involving 109 dental students divided into junior (54) and senior (55) groups, the study compared their diagnostic accuracy against ChatGPT's across seven clinical scenarios. Juniors had the American Association of Endodontists (AEE) terminology assistance, while seniors relied on prior knowledge. Accuracy was measured against a gold standard by experienced endodontists, using statistical analysis including Kruskal-Wallis and Dwass-Steel-Critchlow-Fligner tests. ChatGPT achieved significantly higher accuracy (99.0 %) compared to seniors (79.7 %) and juniors (77.0 %). Median accuracy was 100.0 % for ChatGPT, 85.7 % for seniors, and 82.1 % for juniors. Statistical tests indicated significant differences between ChatGPT and both student groups (p<0.001), with no notable difference between the student cohorts. The study reveals AI's capability to outperform dental students in diagnostic accuracy regarding endodontic assessments. This underscores AIs potential as areference tool that students could utilize to enhance theirunderstanding and diagnostic skills. Nevertheless, the potential for overreliance on AI, which may affect the development of critical analytical and decision-making abilities, necessitates a balanced integration of AI with human expertise and clinical judgement in dental education. Future research is essential to navigate the ethical and legal frameworks for incorporating AI tools such as ChatGPT into dental education and clinical practices effectively.

Pulp regeneration treatment using different bioactive materials in permanent teeth of pediatric subjects

Background and Objectives: The present systematic review aims to assess the success rate of the pulp regeneration treatment, according to the American Association of Endodontists (AAE) criteria, using different bioactive materials in permanent teeth of pediatric subjects (6–17 years of age). Materials and Methods: The study protocol was registered on PROSPERO and adhered to the Preferred Reporting Items for Systematic Reviews and Meta-analyses statement. The question formulation was accomplished using the PICO model, and an electronic search was carried out on Scopus, MEDLINE/PubMed, Web of Science, and Cochrane databases till April 1, 2023. A total of 30 studies were established to fulfill the inclusion criteria of this systematic review. Results: A total of 273 teeth have been treated with pulp regeneration treatment. By comparing different biomaterials and the success criteria defined by the AAE, the material associated with a higher success rate was found to be the white mineral trioxide aggregate. However, the overall success rate of pulp regeneration treatment was reported for 248 out of 273 teeth (91.20%). Conclusions: Data obtained support the potential that regenerative endodontics aids in continuing root development in permanent immature teeth. Further studies are needed for a more extensive evaluation of the use of different biomaterials and the success rate in regenerative endodontics.

Prevalence and reasons for referrals to the Endodontics Specialty Clinic at the Piracicaba Dental School.

Dental referrals to the Endodontics Specialty Clinic (ESC) are routine owing to the complexity of endodontic treatments. To obtain a better prognosis for treatment, students/dentists must perceive their technical limits. This study sought to investigate the referrals of patients to the ESC from different clinics of the Piracicaba Dental School, State University of Campinas - SP, Brazil, and check: a) the demographic profile of patients and the most commonly affected tooth; b) the clinic with the largest number of referrals; c) the reasons for referrals; d) the complexity of the cases; e) and the difficulty in assessing the referred cases based on the classification provided by the American Association of Endodontists (AAE) and Souza-Filho. The study sample consisted of patients' electronic dental referral records from February 2015 to June 2019. A total of 1,707 patients were referred to the ESC during the study period, and 62.4% were female. Lower molars were the most frequently involved teeth (34.8%), and 60.7% of the cases were referred due to the presence of root curvature. The AAE classification showed prevalence of highly difficult cases (71.3%), whereas Souza-Filho classification demonstrated a high rate of class III cases (85.8%). This study highlights the difficulties encountered by undergraduate students before or during endodontic treatments, reinforcing the need for clear criteria for selecting cases appropriate for each education level, thus improving endodontic treatment prognosis.

Applying the American Association of Endodontists and American Academy of Oral and Maxillofacial Radiology guidelines for cone-beam computed tomography prescription: Impact on endodontic clinical decisions

BackgroundThe American Association of Endodontists (AAE) and the American Academy of Oral and Maxillofacial Radiology (AAOMR) developed guidelines for the prescription of cone-beam computed tomographic (CBCT) imaging. The impact of appropriately prescribed CBCT imaging on endodontic diagnosis and treatment (Tx) decisions was examined. MethodsThe clinical databases at the School of Dentistry at the University of California, Los Angeles, Los Angeles, California, were queried to identify patients referred for CBCT imaging from the postgraduate endodontic clinic over a consecutive 36-month period. Primary and secondary indications for CBCT imaging were recorded. Pre-CBCT uncertainty in diagnosis, Tx of the teeth in question, and post-CBCT changes to the diagnosis and Tx plan were recorded. ResultsCBCT imaging was prescribed for 12% of patients. A total of 442 scans were prescribed to evaluate 526 teeth. Molars accounted for 51% of teeth examined. Overall, CBCT effected a change in periapical diagnosis (21%) and in the Tx plan (69%). The 5 most frequent primary indications for CBCT imaging were, in order, AAE-AAOMR recommendations 7, 9, 2, 12, and 6. The impact of these recommendations on Tx decisions varied from 48% through 93%. ConclusionsThis study validates the use of the AAE-AAOMR guidelines for prescribing CBCT imaging for endodontic evaluations. CBCT imaging contributed predominantly to Tx decisions rather than diagnostic determinations. Practical ImplicationsThis study validates AAE-AAOMR case selection guidelines for CBCT imaging and shows a positive impact of prescription imaging on endodontic decision making.

Evaluation of removal efficiency of capping materials used in pulp revascularization in vitro

BackgroundThis study aimed to evaluate the removal efficiency of different capping materials used in pulp revascularization (PR) in a failure scenario.MethodsThe apices of freshly extracted 30 maxillary incisors were cut to mimic the immature teeth; then, root canals were shaped up to #6 Peeso reamers. The regeneration steps of the American Association of Endodontists (AAE) were followed to simulate PR treatment in vitro. The canals were dressed with the Ciprofloxacin and Metronidazole medicament mixture for 2 weeks. Then capping material groups were created: BioDentine (BD), ProRootMTA (PMTA), and RetroMTA (RMTA) (n = 10). The sealed specimens were stored for 2 weeks at 37 ºC in phosphate-buffered saline then the samples were examined by micro-computed tomography (µ-CT) analysis. Set capping materials were retrieved using a specific cement removal kit by a single blind operator. The residue materials were examined again by µCT. Kruskal–Wallis and Mann–Whitney U tests sought the significance for residue volumes. One-way ANOVA and Tukey post hoc tests with the Bonferroni corrections sought significance for the duration (p = 0.05).ResultsIn the first examined µCT data, the mean (SD) capping material volumes of the PMTA, BD, and RMTA were 6.447 µm3 (1.086), 8.771 µm3 (0.491), and 8.114 µm3 (2.447), respectively.In the last examined µCT data, the median (IQR) residual volumes of the PMTA, BD, and RMTA were 0.051 µm3 (0.1), 0.313 µm3 (0.5), and 0.124 µm3 (0.1), respectively. A significant difference was found between BD and PMTA in the residual volumes (p < 0.05).The mean (SD) durations of the retrieving procedures of PMTA, BD, and RMTA were 19.83 min (2.34), 19.24 (3.60), and 22.04 (1.68), respectively (p = 0.063).ConclusionsWithin the limitations of the presented study, it was concluded that the capping materials were largely removed from the root canals using a non-invasive approach. Nevertheless, this duration of the retrieving could be described as long.

A Survey on Cone-beam Computed Tomography Usage Among Endodontists in the United States

IntroductionThe aim of the study was to investigate the accessibility and frequency of cone-beam computed tomography (CBCT) usage and to assess the economic and logistical factors involved with its usage among active American Association of Endodontists (AAE) members, utilizing a web-based survey. MethodsA survey of 19 questions was sent to 3,071 AAE members addressing participant access to, along with financial and logistical aspects of CBCT imaging. Descriptive analysis was performed and Fisher exact test utilized to test associations between groups (P < .05). ResultsThe overall response rate was 14.7% (n = 544). Ninety-five percent of respondents (n = 486) had an in-office CBCT unit, with those graduating after the year 2000 statistically more likely to have one (P < .05). Utilization of CBCT imaging for every case was reported by 40% of providers. Eighty-nine percent reported taking the scan at the consultation visit and 20% included this charge with the consultation fee. For those who charged for the scan separately, 85% charged more than $100. Providers who paid off their unit did so within 1–2 years (41%), 3–4 years (36%), 4–5 years (12%), and 5+ years (11%). Limited field of view was utilized by 95% of respondents. Fifty-eight percent reported interpreting the scans themselves, 38% send only if pathology is expected, and 3% always send their scans to a radiologist. ConclusionsIn conclusion, accessibility and utilization of CBCT imaging among United States endodontists has increased and acquisition of this equipment has not made a long lasting financial burden on providers.

Accuracy of the American Association of Endodontists diagnostic criteria for assessing pulp health in primary teeth.

There is a lack of studies evaluating the accuracy of the 2009 American Association of Endodontists (AAE) diagnostic criteria for diagnosing pulpal health in primary teeth. This study aimed to estimate and correlate the diagnostic accuracy of clinical diagnosis of reversible and irreversible pulpitis using the 2009 AAE criteria with histological findings in primary teeth. Eighty primary teeth that were clinically diagnosed with normal pulp (n = 10), reversible pulpitis (n = 30), irreversible pulpitis (n = 30) and pulp necrosis (n = 10) were collected. The teeth were histo-processed, and pulp tissues were diagnosed histologically as uninflamed pulp, reversible or irreversibly inflamed and necrosis based on previously proposed criteria. The clinical diagnosis of pulp necrosis (sensitivity 70%, specificity 96%) and normal pulp (sensitivity 91%, specificity 100%) matched the histological diagnosis of necrosis and uninflamed pulp in 70% and 100%, respectively. The clinical diagnosis of irreversible pulpitis (sensitivity 64%, specificity72%) matched the histological diagnosis of irreversible pulp inflammation for 47% of teeth evaluated. For the clinical diagnosis of reversible pulpitis(sensitivity: 65%, specificity: 86%), 80% matched the histological diagnosis of reversible pulp inflammation. Teeth with histologically diagnosed irreversible pulp inflammation were more likely to have lingering (OR 5.08; 95% CI 1.48-17.46, P = 0.010) and nocturnal tooth pain (OR 15.86; 95% CI 1.57-160.47, P = 0.019) when compared to teeth with reversible pulp inflammation. Using the classification and regression tree model, the presence of widened periodontal ligament space and nocturnal tooth pain were useful predictors of irreversible pulp inflammation with an accuracy of 78%. The 2009 AAE criteria was acceptable for primary teeth with pulp necrosis and normal pulp but poor for reversible pulpitis and irreversible pulpitis.

A prospective case series in regenerative endodontics: The effective use of diluted antibiotic hydrogels in endodontic regeneration procedures

Objectiveto investigate the effectiveness of diluted antibiotic hydrogels in endodontic regeneration procedures. Materials and MethodsOne conventional treatment (calcium hydroxide) and two experimental treatments containing 1 mg/mL antibiotic in gel (MC-TAP and MC-DAP) were prepared for this study. The effect of these medicaments on the proliferation and differentiation potential of dental pulp stem cells (DPSCs) was examined before their clinical use, using WST and ALP assays. Twenty-two teeth diagnosed with necrotic immature roots were treated with calcium hydroxide, MC-TAP or MC-DAP using the endodontic regeneration protocol as described by the American Association of Endodontists (AAE). Teeth were examined after application of the medicament, at the time of regeneration and after 12–24 months. ResultsAll treated teeth showed resolution of signs and symptoms shortly after treatment with the different medicaments. However, signs and symptoms returned a few months after initial treatment in clinical cases treated with MC-DAP. All successfully treated teeth examined after 12–24 months were free of signs and symptoms, but vitality was not achieved. ConclusionLow concentration antibiotic gel containing triple antibiotic paste (MC-TAP) can be successfully used for endodontic regeneration procedures.

Vital pulp therapy for permanent teeth with diagnosis of irreversible pulpitis: biological basis and outcome.

Root canal treatment (RCT) has been considered the conventional standard for the management of teeth with carious pulp exposure, particularly in mature teeth presenting with symptoms. Following a better understanding of the histopathology of deep carious lesions, the histology of the cariously exposed pulp and the healing potential of the inflamed pulp, vital pulp therapy (VPT) is increasingly adopted around the world for the management of permanent teeth with clinical signs and symptoms indicative of irreversible pulpitis. Furthermore, VPT became a recognized treatment modality by the European Society of Endodontology (ESE) and the American Association of Endodontists (AAE) by virtue of its high success rates reported in outcome studies using contemporary hydraulic calcium silicate-based cements. However, proper case selection, strict asepsis, capping materials and good coronal seal are mandatory for success. The aim of this paper is to review the biological basis for VPT in symptomatic teeth with carious pulp exposure and to report on the outcome of pulpotomy in teeth with clinical diagnosis of irreversible pulpitis.

Clinical and radiographic outcomes of regenerative endodontic procedures for traumatized permanent necrotic teeth with apical periodontitis and external root resorption.

This clinical study was undertaken to assess the effect of treatment with regenerative endodontic procedures (REPs) on 20 teeth with pulp necrosis, apical periodontitis, and external root resorption (ERR). Teeth were treated with REPS utilizing the American Association of Endodontists (AAE) protocol. Quantitative assessment of changes in radiographic root area (RRA) were statistically analysed to assess changes in root dimensions after an average follow-up period of 3 years. All 20 teeth survived, 14 teeth (70%) were classified successful, and only 1 tooth (5%) failed throughout the study period. Based on the radiographic examination, all 20 teeth showed complete repair of the periapical lesions and arrested ERR. However, 5 teeth (25%) subsequently developed replacement resorption. The RRA between baseline and 3-year follow-up showed a significant difference for the total 20 teeth (p = .009). An analysis according to the trauma type and the extra-oral time showed the RRA increase was significantly different in the non-avulsion group (p = .015) and for the avulsion group with an extra-oral time less than 60 min (p = .029). The RRA increase was not statistically significant in the avulsion group of extra-oral time more than 60 min (p = .405). Nine teeth (45%) and 10 teeth (50%) responded to cold and electric pulp testing, respectively. Within the limitations of this study, the favourable outcomes of REPs were further confirmed for traumatized permanent necrotic teeth with ERR in terms of periapical lesion healed and a significant increase in RRA. The study contributes further evidence of the role of REPs in arresting ERR.

Evaluation of the characteristics of root canal calcification after regenerative endodontic procedures: A retrospective cohort study over 3 years.

This study aimed to evaluate the characteristics of root canal calcification after regenerative endodontic procedures (REPs) during long-term follow-up. Data of children who underwent REPs and were followed up for >3 years in the Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, China, from January 2013 to January 2019, were collected. All the patients were treated by the protocol of REPs based on the American Association of Endodontists (AAE) protocol. A total of 91 teeth of 54 boys and 37 girls (average age 10.4 ± 1.9 years) with follow-up duration >3 years were included. The follow-up duration ranged from 36 to 92 months (average, 53.2 ± 13.4months). The prevalence, contributing factors, and long-term prognoses of root canal calcification after REPs are discussed. Independent t-test and χ2 test were used for statistical analysis. The incidence of root canal calcification was 78% (71/91). The use of calcium hydroxide paste was significantly correlated with the occurrence of root canal calcification (p < .05). Some teeth showed aggravation of calcification with time; however, not all teeth showed calcification after longer follow-up duration. Teeth treated with REPs had a relatively high probability of root canal calcification detection during the long-term follow-up. The occurrence of calcification is related to the use of calcium hydroxide paste but does not affect the long-term prognosis of teeth.

MICRO-COMPUTED TOMOGRAPHY ASSESSMENT OF THE ROOT CANAL SYSTEM

Introduction: The root canal system (RCS) is among the complex parts of the body that consists of several anatomical variations. The RCS has also been classified according to different classifications. In one of the classification systems, the RCS consists of the main canal, lateral canal, collateral canal, secondary canal, intercanal, accessory canal (AC), and recurrent canal, whereas in the other classification system, which was presented by the American Association of Endodontists (AAE) in 2020, the RCS consists of the C-shaped canal, lateral canal, furcation canal, and AC. Many researches have already been conducted on using micro-CT in describing the RCS classification in various teeth groups in the jaws. The micro-CT is a method used for the endodontic study, including the morphological study of the RCS. It is one of the non-destructive and high-resolution techniques that is beneficial in obtaining three-dimensional (3D) teeth structures. The method is similar to computed tomography and allows obtaining sections as well as 3D reconstruction of the studied object. Based on the above-mentioned findings, the authors discussed that canals are usually more long oval or ribbon-shaped at the coronal levels, then become oval or round shaped about 1 mm from the apical foramen, indicating that canal shape and number change in the apical third. Conclusion and future perspectives: The micro-CT could be a useful technology in the clinical study of canals, developmental grooves, and comparison of treatment and diagnostic strategies.

Diagnosis consensus among endodontic specialists and general practitioners: An international survey and a proposed modification to the current diagnostic terminology.

This survey aimed to determine the consensus amongst endodontic specialists in North America and practitioners worldwide to diagnose the pulp and periapical conditions of selected case scenarios encountered in daily practice using the American Association of Endodontists (AAE) pulpal and periapical diagnostic terms. Secondly, an attempt was made to suggest modifications in terms accordingly. A survey designed by two endodontic educators was sent to endodontists in North America and clinicians worldwide through an electronic database. The survey included socio-demographic questions followed by the clinical and radiographic presentations of four clinical scenarios. The participants were then requested to provide the pulpal and the periapical diagnosis of 11 teeth presented in these cases (22 answers in total/participant) using the AAE diagnostic terminology. Cases were designed to include 12 pulpal/periapical conditions as control (non-controversial conditions) and ten so-called controversial conditions. A proportion threshold of 10% was required for any diagnostic term to be reported in this survey. The participants were divided into two groups based on the region of endodontic training and/or practice to 'Specialised North American' or 'International Practitioners,' and their results were statistically compared using chi-squared tests (p< .05). The survey included 421 participants. 74% were endodontists, and 46.1% were amongst the 'Specialised North American' group and 53.9% amongst the 'International Practitioners'. Eleven of 12 control conditions had an almost complete agreement amongst the participants regarding the diagnostic terms selected, ranging between 82% and 96%, with no other diagnostic term exceeding the 10% threshold. All the controversial conditions yielded more than one diagnostic term selected/condition that exceeded the 10% threshold for groups ('Specialised North American' and 'International practitioners'). There were no differences in the diagnostic terms selected between the two groups; however, the weight for each term varied between the groups in some cases. There is a lack of consensus amongst clinicians, regardless of their training and region of practice, on the appropriate diagnostic terms to be used in particular clinical conditions. More diagnostic terms and modifications in the current terms may be required to establish a more reliable diagnostic terminology.

What is the best effective postoperative medication in reducing pain after non-surgical root canal treatment?

Data sources Medline, Embase, CENTRAL, Cumulative Index to Nursing and Allied Health Literature and Scopus databases were searched (until July 31 2019). They searched the WHO International Clinical Trials Registry Platform and ClinicalTrials.gov databases for completed and ongoing trials, as well as ProQuest, Google Scholar (first 100 hits) and the OpenGrey database for those unpublished. In addition, abstracts of annual meetings from the American Association of Endodontists, the International Federation of Endodontic Associations and the European Society of Endodontics were searched. A manual search of references in related papers and textbooks was carried out. Any missing information was filled in by contacting the authors.Study selection All randomised clinical trials (RCT) of nonsurgical endodontic therapy with pharmaceutical treatments provided postoperatively to alleviate pain on adult subjects were eligible. Two reviewers selected the studies and extracted the data and the results were examined by a third reviewer. Any differences were settled with the help of a fourth reviewer.Data extraction and synthesis Two authors independently evaluated and retrieved data from chosen trials found through searches. Another reviewer looked at the trial selection and data extraction. They also assessed the risk of bias in each of the research they chose.The mean and standard deviation of a 0-100 (mm) visual analogue pain scale were extracted or derived from study text, graphs and tables The postoperative medications were compared using network meta-analysis in a Bayesian model. Mean differences (MDs) and 95% credible intervals (CrIs) of post-operative pain were estimated at 6-8 hours and 12, 24 and 48 hours. Two authors utilised the CINeMA web application to assess the confidence in the main results by accounting for six domains: within- and across-study bias; indirectness; imprecision; heterogeneity; and incoherence. Each domain was assigned no concerns, major concerns, or minor concerns. Each result would be assigned a confidence level of high, moderate, low, or very low. To establish an agreement, a third reviewer was consulted.Results From the initial search of 969 records, 11 RCTs matched the inclusion criteria. Five studies were rated as having a low risk of bias, five as having a moderate risk and one as having a high risk. Moderate and major heterogeneity and some inconsistencies were detected, though not statistically significant.Oral medications were classified into placebo, non-steroidal anti-inflammatory drugs (NSAIDs), opioids, acetaminophen, NSAIDs + acetaminophen, corticosteroids, NSAIDs + benzodiazepines and NSAIDs + opioids groups.With moderate confidence, NSAIDs + acetaminophen were shown to be more efficacious than placebo at 6-8 hours following nonsurgical root canal therapy (MD = 22; 95% CrI = [-38, -7.2]). With very low confidence, NSAIDs had a greater effect than placebo after 12 and 24 hours (MD = -28; 95% CrI = [-49, -7] and MD = -15; 95% CrI = [-27, -2.3], respectively). After 6 12 and 24 hours, other medications were no more effective than placebo in pain reduction. After 48 hours, no treatment was more effective than placebo. Subgroup analysis found that other medications, such as corticosteroids and acetaminophen, were no more beneficial than placebo for patients who could not use NSAIDs. Six trials provided safety data and concluded that the therapies were safe and posed no significant risks.Conclusions Very low to moderate-quality evidence suggests that postoperative administration of NSAIDs and acetaminophen, or NSAIDs alone, reduced discomfort after non-surgical root canal therapy in patients with irreversible pulpitis or pulpal necrosis. Trials have shown, however, that using these drugs does not result in major complications. We cannot, however, be confident that they are risk-free.

Advanced Electrochemical Reamer (EC-Reamer) for Root Canal Treatment.

According to the American Association of Endodontists (AAE), 22 million endodontic procedures have been performed annually. Root canal treatment is needed to prevent infection and restore function when a tooth is severely infected or decayed. This procedure is the only way to preserve the natural tooth and avoid artificial replacement (implant, denture, etc.). The current study aims to develop an electrochemical reamer (EC-Reamer or EC-R) that can help to disinfect the canal system and thus improve the success rate of root canal treatment. The COMSOL Multiphysics software was utilized to simulate the experimental setup and confirm the current flow in the electrolyte. The benchtop experimental approach follows a specific electrochemical protocol, (i) open circuit potential to monitor the electrochemical stabilization and (ii) potentiostatic scan at -9.0 V as the treatment stage. Identification of feasible reference electrode (RE) and insulation material for the exploratory benchtop studies considered platinum (Pt) and gold (Au) wire as the REs and hot melt adhesive (HMA) and liquid tape as the insulation materials. The antimicrobial effects of EC-R were analysed using Enterococcus faecalis (E. faecalis). One-way ANOVA with the Tukey post hoc test and a significance level of P<0.05 is used to compare the groups with an experimental duration of 60 seconds. The findings showed that magnitude and current fluctuations created by Pt wire are promising when compared to Au wire, while Pt-HMA pair is chosen considering Pt's good electrochemical inertness and HMA's easy handling, availability, and non-hazardous features. The use of potentiostatic duration of 1 s and 3 s resulted in >99.99% E. faecalis reduction. Duration at 5 s and above resulted in a total bacterial kill. Statistical analysis confirmed a significant difference among the groups tested with commercial and custom-built potentiostats. The outcome provided preliminary data for developing an EC-R prototype to enhance the antimicrobial effect during root canal treatment potentially. (EEJ-2022-01-04).

International questionnaire study on systemic antibiotics in endodontics. Part 1. Prescribing practices for endodontic diagnoses and clinical scenarios.

Part 1 of this two-part study aims to investigate systemic antibiotics (SA) prescribing practices for various endodontic diagnoses and clinical scenarios by general practitioners, post-graduate students and endodontists in Israel and the former Soviet Union (FSU) states. A total of 7500 dentists from Israel and FSU states were invited to fill in an online questionnaire. The questionnaire consisted the SA prescribing for endodontic diagnoses and clinical scenarios (post operative pain, post apical surgery and post avulsion). The international guidelines of the European Society of Endodontology and the American Association of Endodontists were used as the standard of correct practice. A total of 1310 dentists (response rate of 17.46%, 498 Israeli and 812 FSU dentists) completed the survey. The rates of prescription SA among the Israeli and the FSU respondents for non-recommended diagnoses and clinical scenarios were 24.75% and 11.42% (P>0.05), 16.57% and 16.17% (P>0.05) respectively. The rates of prescription SA among the Israeli and the FSU respondents for recommended diagnoses and clinical scenarios were 63.39% and 62.9% (P>0.05), 27.2% and 13.9% (P>0.05) respectively. There are gaps between the recommended protocols for prescribing SA for patients with various endodontic diagnoses and clinical scenarios and the actual practices among Israeli and FSU dentists. Continuing education of dentists must be encouraged in order to improve SA prescription practice according to international guidelines. When not indicated, prescription of systemic antibiotics might lead to antimicrobial resistance, while non-prescription, when required, might have negative repercussions on the patient's health. Knowledge of the prescription habits of dentists in the endodontic practice will help prevent harmful situations.

A prospective clinical study investigating the effectiveness of partial pulpotomy after relating preoperative symptoms to a new and established classification of pulpitis

To prospectively investigate the outcome of partial pulpotomy after 1year, using a hydraulic calcium silicate cement (HCSC) on symptomatic cariously exposed pulps in adult teeth. To compare the traditional American Association of Endodontists (AAE) pulpitis classification with the recently proposed Wolters classification system in predicting the likelihood of treatment failure. Sixty-two symptomatic adult teeth with deep and extremely deep carious lesions were classified according to the Wolters (mild/moderate/severe pulpitis) and the traditional pulpitis classification (reversible/irreversible pulpitis). Eleven teeth were excluded intraoperatively as there was no pulp exposure after non-selective caries removal. The remaining 51 teeth, regardless of diagnosis, were treated by partial pulpotomy, pulpal lavage with 2.5% sodium hypochlorite solution, haemostasis and HCSC application (Biodentine™) as a pulp capping material. A permanent restoration was placed during a second appointment 1-2weeks later. Preoperative tenderness to percussion (TTP), bleeding time and material setting time were recorded as was preoperative and postoperative tooth colour under standardized conditions. Clinical review occurred at regular intervals with clinical/radiographic analysis at 12months. Chi-square analysis and Fisher's exact test assessed different outcomes amongst the diagnostic categories; the Kruskal-Wallis and Wilcoxon rank-sum test assessed influence of pulp bleeding time, TTP or variation in setting time (p<.05). Ten cases were lost to review, and a total of 41 teeth were reviewed at 1year and classified as either "success," "successful but unresponsive to sensibility testing" or "failed." This included five severe, 17moderate and 19mild pulpitis according to Wolters classification or 23 reversible pulpitis and 18 irreversible pulpitis cases by the AAE classification. The majority of the 62 enrolled cases were "extremely deep" (n=50), rather than "deep" (n=12) caries with all failures occurring in the extremely deep group. Partial pulpotomy was 90% successful (100% reversible, 78% irreversible or 100% mild, 88% moderate, 60% severe pulpitis) with a significant difference in outcome between mild and severe pulpitis groups (p=.04). Only one, severe pulpitis/irreversible pulpitis, case failed painfully prior to the 1-year review appointment. Bleeding time (p=.26) and TTP (p=.61) did not influence treatment outcome, whilst Biodentine™ setting time was significantly longer than manufacturers' claim (p<.05). No teeth discoloured. Partial pulpotomy using Biodentine™ was successful for treating symptomatic carious pulpal exposures after 1year, but included cases where pulp vitality could not be confirmed. Within the limitations of this study, cases with signs and symptoms indicative of irreversible pulpitis were not less successful; however, Wolters classification highlighted severe pulpitis to be less successful than mild pulpitis, thereby providing a potential prognostic benefit in diagnostically subdividing pulpitis. Caries depth was an indicator of failure, whilst bleeding time and preoperative tenderness to percussion were not.

The Evolving Landscape of the American Board of Endodontics Certification Process: What it Means for the Future of the Specialty

IntroductionThe American Board of Endodontics certification is an evolving process to ensure the continuing existence of the specialty of endodontics. Recent examination changes have resulted in greater numbers of Diplomates. To fully understand the implications of such changes and evaluate current Diplomates' views of the examination process, a specific set of questions was developed focusing on beliefs and perceptions, age relationships, test components, and hypotheticals for potential changes. An analysis of the examination process has never been reported in the literature, and, as such, the survey was designed to provide the endodontic community with critical information on the past, current, and potentially future examination process. MethodsA Web-based survey consisting of 25 questions was e-mailed to 1522 Diplomates. ResultsA total of 736 Diplomates responded to the survey for a response rate of 48.4%. Descriptive statistics and regression analysis (p < .05) were used to explore relationships in the data. ConclusionsA lack of perceived importance of being board certified was a major barrier. Respondents opposed de-emphasizing literature, offering the oral examination in a videoconferencing virtual format, eliminating mandatory recalls, and entirely removing 1 component of the examination to increase the numbers of Diplomates. Respondents supported reviewing the case history portfolio examination on a rolling basis. Respondents disagreed that molar cases alone demonstrated clinical competency. Older Diplomates felt the strongest that the meaning of being a Diplomate has been diluted by the changes in the certification process. The authors believe the American Association of Endodontists, program directors, and the College of Diplomates must continually emphasize the importance and value of board certification as the process continues to evolve.

Antibacterial Efficacy of Triple Antibiotic Medication With Macrogol (3Mix-MP), Traditional Triple Antibiotic Paste, Calcium Hydroxide, and Ethanol Extract of Propolis: An Intratubular Dentin Ex Vivo Confocal Laser Scanning Microscopic Study

IntroductionThis study aimed to assess the effectiveness of antibacterial activity of medications used in regenerative endodontic treatment. MethodsSixty-seven dentin cylinders of single-rooted teeth were contaminated with a culture of Enterococcus faecalis (ATCC 29212; American Type Culture Collection, Manassas, VA) for 5 days. Samples were divided into 1 control group and the following experimental groups according to the medication applied: traditional triple antibiotic paste (TAP), clindamycin-modified TAP (mTAP), triple antibiotic medication with macrogol (3Mix-MP), clindamycin-modified 3Mix-MP (m3Mix-MP), calcium hydroxide (CH), and ethanol extract of propolis (EEP). After 14 days, the medications were removed, and the samples were submitted to confocal laser scanning microscopic analysis to quantify the percentage of viable bacteria. The distribution of data was confirmed by the Shapiro-Wilk test. The Kruskal-Wallis and Dunn tests were used for intergroup comparisons, and the Wilcoxon test was used for comparison between superficial and deep antibacterial efficacy for the same medication. The level of significance was set at P < .05. Results3Mix-MP and m3Mix-MP presented significantly higher antibacterial efficacy compared with the other tested medications (P < .05), except for mTAP. mTAP was more effective than TAP (P < .05). The antibacterial efficacy of EEP and CH did not differ significantly from TAP and mTAP (P > .05). All medications showed effective antibacterial action compared with the control group (P < .05). Conclusions3Mix-MP and m3Mix-MP, which present extremely high concentrations of antibiotics (1500 mg/mL), were not more effective than mTAP at the concentration recommended by the American Association of Endodontists (5 mg/mL). Moreover, CH and EEP were as effective as TAP and mTAP.