Oral Mucosa Research Articles

The impact of interactions between heavy metals and smoking exposures on the formation of oral microbial communities

IntroductionThe primary objective of our investigation was to assess the repercussions of prolonged exposure to heavy metals and smoking on the microbiome of the oral buccal mucosa. Concurrently, we aimed to elucidate the intricate interplay between external environmental exposures and the composition of the oral microbial ecosystem, thereby discerning its potential implications for human health.MethodsOur study cohort was stratified into four distinct groups: MS (characterized by concurrent exposure to heavy metals and smoking), M (exposed solely to heavy metals), S (exposed solely to smoking), and C (comprising individuals serving as a control group). Specimens of buccal mucosa and blood were systematically acquired from the participants, facilitating subsequent microbial diversity analysis across the four oral buccal mucosa sample cohorts through 16S rRNA gene sequencing techniques. Simultaneously, blood samples were tested for heavy metal concentrations. In addition, we performed topological analyses by constructing microbial networks.ResultsOur findings notably indicate that co-exposure to heavy metals and smoking yielded a more pronounced alteration in the diversity of oral microflora when compared to singular exposures to either heavy metals or smoking. By comparing the oral bacterial communities and functional pathways between the four groups, we found significant differences in bacterial communities and functional pathways between the groups. Notably, the impact of heavy metal exposure overshadowed that of smoking, with concurrent exposure to heavy metals and smoking eliciting marginally greater effects than exposure to heavy metals alone. In addition, our analysis of the correlation between microbiota and blood heavy metal concentrations showed that the heavy metal cadmium (Cd) had a significantly greater effect on oral microbiota than other heavy metals.DiscussionChronic exposure to heavy metals and smoking disrupts the normal bacterial communities in the oral mucosa of residents of contaminated areas. This exposure reduces the complexity and stability of microbial networks and increases the risk of various diseases reduces the complexity and stability.

Enhancing Buccal Drug Delivery: The Impact of Glycerol in Slot-Die-Coated Pectin Films.

Buccal delivery offers a promising alternative to e.g., oral or parenteral drug administrations by leveraging the mucosal membranes of the mouth to enhance drug absorption and enhance patient compliance. Buccal films offer a promising approach for enhancing drug delivery by utilizing the mucoadhesive properties of the biopolymer pectin and glycerol's plasticizing effects. Designed to provide fast drug release, these films address the challenges of patient compliance, particularly among the elderly, children, and individuals with dysphagia. This study characterized the physicochemical properties of slot-die-coated films with pectin containing varying amounts of glycerol, including swelling behavior, disintegration rate, mechanical properties, mucoadhesion, and drug release profiles, using paracetamol as a model drug. Different methods such as quartz crystal microbalance with dissipation and open-source force analyzer were employed for the characterization. The results demonstrated that a high glycerol content in the films led to slower drug release with 95% paracetamol released for film without glycerol (GLY0) compared to only 74% released for film with 20% w/v glycerol (GLY20) after 60 min Additionally, higher glycerol levels resulted in enhanced mucoadhesive properties. Films containing 20% glycerol also showed superior permeability of paracetamol through ex vivo porcine buccal mucosa, with double the amount of paracetamol permeating in the first 120 min from GLY20 films compared to GLY0 films. These findings suggest that the pectin-glycerol buccal films, fabricated with slot-die coating as a novel technique, are user-friendly, exhibit interaction with the mucosa, and can be adjusted for specific disintegration and drug release rates, presenting a promising option for efficient, targeted drug delivery.

Morphometric analysis of epithelium in oral submucous fibrosis using open-source software

Background: Oral submucous fibrosis (OSMF) is a chronic oral mucosal disease characterized by progressive deposition of collagen in subepithelial connective tissue and epithelial atrophy. ImageJ is open-source software that aids researchers to visualize, inspect, quantify, and validate scientific image data. Objectives: To measure and compare the changes in epithelial thickness and cell morphology in OSMF in comparison with normal mucosa using computer-aided image analysis software. Materials and Methods: 15 OSMF and 10 cases of normal buccal mucosa were analyzed. Epithelial thickness and cell morphology of spinous cells of OSMF cases were quantified by ImageJ software and compared with that of normal tissue. Results: An increase in the cellular area of the spinous cells in OSMF compared to that of the normal tissue was observed. Conclusion: ImageJ represents a powerful tool that enhances the precision and efficiency of epithelial analysis in OSMF research compared to historical methods.

The Effect of Five Disinfection Methods on Candida albicans-Infected Chair-Side Soft Liners: A Comparative In Vitro Study

Background: Soft-liners, particularly used for patients with poor hygiene habits, can become a favorable environment for the aggregation of microorganisms. Therefore, disinfection is considered a critical process for preserving the health of oral mucosa. This study aimed to determine the antifungal efficacy of five different disinfectant methods on silicone resilient lining materials. Material and Method: A total of 60 test samples were prepared and inoculated with 2 ml of Candida solution and incubated for 2 hours. The inoculated specimens were then randomly divided into six groups (n=10): Control group, 0.5% sodium hypochlorite (NaOCl) group, Corega® denture cleansing tablet group, microwave water group, diode laser group, and 4% iodine solution group. A swab was taken from the surfaces of the soft-liner discs in each group, planted on aerobic culture media, and the colonies were counted separately for each treatment. Results: A significant difference (p < 0.05) in inhibiting the growth of Candida albicans was observed with all five types of disinfection methods compared to the control group. Conclusion: According to the results, the iodine group and the laser group demonstrated the best disinfection effects, respectively.

Clinico-pathological spectrum of oral cavity lesions with their radiological, histopathological, and immuno-histochemistry correlation at a tertiary care teaching hospital: A study of 200 cases

: The oral cavity is vulnerable to different types of environmental insults and chemical injuries specifically with the use of tobacco. Among the various oral cavity lesions, oral squamous cell carcinoma (SCC) is a major burden in the developing countries. Diagnosis is done mainly by histopathological examination while few cases need immunohistochemistry (IHC). Some neoplastic lesions are small and deep-seated and need CT or MRI for further evaluation. This study was aimed at studying the spectrum of oral cavity lesions with respect to age, gender and location and to analyse their radiological and histopathological aspects.: The aim of this article is to study the spectrum (neoplastic and non-neoplastic) of the oral cavity lesions with respect to age, gender, and location/site distribution, to analyse the radiological aspect of the oral cavity lesions and to analyse the histopathological aspect of the oral cavity lesions.: This is a retrospective and prospective study of 200 cases over a period of one and a half years. The information like age, gender, etc. was recorded from the requisition forms and the departmental record and studied statistically. p63, CD45, CD19, CD20 IHC markers were done.: The most involved age group was 41 to 50 years. The male: female ratio was 2.27:1. Out of 200 studied cases, 102 (51%) were malignant lesions. In SCC, the buccal mucosa was the most common site with ulcero-proliferative presentation and association with tobacco consumption. : Histopathological examination is the gold standard for the diagnosis of oral cavity lesions. IHC is helpful to confirm an epithelial lineage. Prevention as well as early detection and treatment of oral SCC improves the lifestyle of affected individual and reduce the overall morbidity and mortality associated with oral SCC.

Primary Osseointegrated Free Flap in a Middle-Aged Oral Cancer Patient with Excellent Functional and Anatomical Outcomes: A Case Report

Squamous cell carcinoma (SCC) of the oral cavity is a significant global health concern, accounting for approximately 90% of all oral malignancies. This case report describes a 45-year-old male diagnosed with SCC of the right alveolus sulcus extending to the buccal mucosa. The treatment involved a multi-disciplinary team employing advanced planning and surgical techniques, including 3D Computer-Aided Design (CAD) and Computer-Aided Manufacturing (CAM) technology, for precise preoperative planning of the composite resection and reconstruction. This approach facilitated accurate mandibular resection, fibula flap harvest, and immediate dental implant placement, paving the way for future prosthetic rehabilitation. This report highlights the importance of comprehensive treatment planning and the integration of cutting-edge technologies in managing oral SCC. The outcome was a scarless, contoured face with excellent anatomical and functional outcomes.

Exploring the Uncommon, Tongue Schwannoma—A Rare Case Report

Schwannoma (neurilemmoma or neurinoma), is a benign tumor arising from Schwann cells that comprise the myelin sheaths surrounding peripheral nerve. They are encapsulated, usually solitary and slow-growing. Approximately 25%-45% of schwannoma occurs in the head and neck region. Intraoral incidence of schwannoma is 1%-12%. The most common intraoral site is tongue (1%) followed by palate, floor of the mouth, buccal mucosa, gingiva, lips and vestibular mucosa, retromolar region is the least common site. We describe a rare case of lingual schwannoma involving the left lateral part of tongue in a young male presented with a slow-growing, asymptomatic, painless mass on the left lateral part of tongue for several years. Magnetic resonance images demonstrated a well-defined almost oval-shaped isolated mass with uniform intensity on T2 weighted images relative to surrounding muscles. Patient was managed with complete surgical excision of the tumor and has not shown any recurrence in the follow-up period of 1 year. Schwannoma of tongue is a rare tumor of head and neck region. The diagnosis should be based on histopathological examination and in some cases after immunohistochemistry findings. Complete transoral resection allows removal of tumor to avoid recurrence and also avoids causing morbidity of tongue function.

Elemental composition of sputum, immune status, and features of pharyngeal microbiota in workers of the anode department of a copper smelter

The development of bronchial and pulmonary pathology depends on characteristics of the aerosols and individual resistance of the body, largely determined by the immune system, to their exposure. Mucous membranes of a healthy person are colonized by saprophytic bacteria or normal microbiota. Yet, changes in the composition and properties of the microbial landscape induced by adverse environmental factors increase the risk of inflammation and contribute to changes in the reactivity of the immune system. The study aims to establish the immune status, microbiota of the pharyngeal mucosa, and the elemental composition of sputum, given its cellular composition, in workers of the anode department of a copper smelter. We tested sputum culture and elemental composition, established clinical and immunological status, and analyzed pharyngeal microbiota in 28 workers of the anode department of a copper smelter. Based on the results of testing, the subjects were divided into two groups with (1) and without (2) leukocytosis (>10 cells in the field of view) detected in sputum. We found no acute or chronic diseases of the bronchi and lungs in the examined workers. In group 1, we observed shifts in the immune response manifested by a decrease in the number of immunocompetent cells, activation of neutrophil phagocytosis, formation of reactive oxygen species in neutrophils, and displacement of normal microbiota of the oropharyngeal mucosa by opportunistic microorganisms (in 100% versus 35%, p<0.05). The elemental composition of sputum was noted for a higher frequency of iron and lead detection and increased concentrations of barium, lead, iron, arsenic, molybdenum, antimony, vanadium, and cadmium. We established a significant effect of metals contained in sputum on cellular, humoral, and local immunity. The inflammatory bronchopulmonary response to toxic aerosol exposure is accompanied by changes in the cellular and phagocytic links of immunity, as well as in the microbial landscape on the mucous membrane of the upper respiratory tract characterized by displacement of normal biota by opportunistic microorganisms. Limitations. This study has certain limitations associated with the small sample size and the lack of opportunity to compare metal concentrations found in sputum with reference levels. Ethics. The study was approved by the Local Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers (extract from the minutes of the meeting No. 8 dated 12/26/2016).

Pemphigus and Bullous Pemphigoid Following COVID-19 Vaccination: A Systematic Review

The COVID-19 pandemic has encouraged the rapid development and licensing of vaccines against SARS-CoV-2. Currently, numerous vaccines are available on a global scale and are based on different mechanisms of action, including mRNA technology, viral vectors, inactive viruses, and subunit particles. Mass vaccination conducted worldwide has highlighted the potential development of side effects, including ones with skin involvement. This review synthesizes data from 62 manuscripts, reporting a total of 142 cases of autoimmune blistering skin diseases (AIBDs) following COVID-19 vaccination, comprising 59 cases of pemphigus and 83 cases of bullous pemphigoid. Among the 83 bullous pemphigoid cases, 78 were BP, with additional cases including 2 oral mucous membrane pemphigoid, 1 pemphigoid gestationis, 1 anti-p200 BP, and 1 dyshidrosiform BP. The mean age of affected individuals was 72 ± 12.7 years, with an average symptom onset of 11 ± 10.8 days post-vaccination. Notably, 59% of cases followed vaccination with BNT162b2 (Pfizer-BioNTech), 51.8% were new diagnoses, and 45.8% occurred after the second dose. The purpose of our review is to analyze the cases of pemphigus and bullous pemphigoid associated with COVID-19 vaccination and to investigate the pathogenetic mechanisms underlying the new development or flare-up of these diseases in association with vaccination. Our results show that the association between COVID-19 vaccines and AIBDs is a possible event.

The Effectiveness of Salivary Sampling for the Detection and Quantification of Aggregatibacter actinomycetemcomitans in Periodontitis Patients

The objective was to evaluate using unstimulated saliva in detecting Aggregatibacter actinomycetemcomitans and to compare the saliva and subgingival and mucosa membrane occurrence of this periodontal pathogen in patients diagnosed with advanced periodontitis. Patients with advanced forms of periodontitis (n = 220; mean age: 54.03 ± 03 years) at stage III/IV were sampled. Unstimulated saliva, buccal cheek mucosa, and pooled subgingival plaque samples were collected. The identification of A. actinomycetemcomitans was performed using qPCR. A descriptive analysis and Wilcoxon test and analysis of variance were performed. A. actinomycetemcomitans was isolated from 28.18% of the subjects. A total of 660 samples were obtained, 220 from unstimulated saliva, 220 from buccal cheek mucosa surfaces, and 220 from pooled subgingival plaque samples. A. actinomycetemcomitans was isolated from 21.80% of unstimulated saliva, 19.50% of buccal cheek swabs, and 17.70% of subgingival samples. There was a statistically significant difference between the presence of A. actinomycetemcomitans in the unstimulated saliva samples and in the buccal cheek mucosa swab samples and pooled subgingival plaque samples (p < 0.001). These results suggest that in advanced periodontitis, unstimulated saliva is representative of pooled subgingival plaque/buccal cheek mucosa samples and its use is adequate in the oral detection of A. actinomycetemcomitans in a cohort of patients with stage III and IV periodontitis.

DEMOGRAPHIC PROFILE OF ORAL LICHEN PLANUS PATIENTS IN THE BULGARIAN POPULATION. CLINICOPATHOLOGICAL CORRELATION IN THE DIAGNOSIS OF THE DISEASE

Oral lichen planus (OLP) is a relatively common mucosal disease that mainly affects middle-aged women, yet the epidemiological characteristics of this condition in the Bulgarian population are poorly studied. OLP is considered a clinic-pathological diagnosis that is sometimes challenging to make. Aim. The aim of the present study was to determine the prevalence of OLP among the Bulgarian population and to evaluate the clinico-pathological correlation in the diagnosis of the disease. Materials and Methods. The files of the patients with diseases of the oral mucosa consulted in the Department of Periodontology and Oral Mucosa Diseases, Medical University of Plovdiv, between 2012-2016, were retrospectively reviewed. A comparison was also made of the clinical and histopathological diagnoses retrieved from the archived pathohistological records for the same cohort of patients. Results. Out of a total of 714 patients with mucosal diseases, 141 have been diagnosed with OLP, with the disease accounting for the largest proportion (20%) among the analyzed group. Male to female ratio was 1:4, and most patients were over 51 years of age. Histological confirmation of the clinical diagnosis OLP has been obtained in 84.1%, and there were additional 10.84% ​​cases with a histological diagnosis of OLP that were clinically misdiagnosed. Conclusion. OLP seems to be of great clinical importance in our country. There is a clinic-pathological discrepancy in the diagnostic evaluation of the disease in a relatively high percentage of cases. Therefore, stricter diagnostic criteria need to be introduced and followed to obtain a more reproducible diagnosis of OLP.

Effect of CAD/CAM Abutment Morphology on the Outcomes of Implant Therapy: A Randomized Controlled Trial.

Variations in transmucosal abutment contour design may affect the outcomes of implant therapy. This randomized controlled trial was primarily aimed at testing the effect that CAD/CAM zirconia abutments with either a concave or linear divergent transmucosal morphology have on peri-implant mucosal dynamics and indicators of peri-implant health at 1 year after final implant-supported prosthesis insertion in the anterior maxilla. Following computer-guided implant placement and osseointegration, eligible subjects were randomized into either the experimental (concave morphology) or the control (linear divergent morphology) group. A comprehensive set of outcomes of interest related to peri-implant soft tissue dynamics, phenotypical features, and indicators of peri-implant health were assessed at different time points over a 1-year period after insertion of the final restoration. Out of 60 initially recruited subjects, a total of 54 completed the study (n = 29 in the experimental group concave/n = 25 in the control group). Overall implant survival and restoration rates between master impression and 12 months were 100% and 98.2%, respectively. Although a trend for coronal migration of the buccal mucosa zenith, gain in mucosal thickness, and increased probing depth and bleeding on probing was observed in both groups, these changes were clinically negligible, and no substantial differences were observed between study groups regardless of variations in transmucosal abutment morphology. The use of either linear divergent or concave custom CAD/CAM zirconia abutments in a screw-retained, delayed loading approach yielded no significant differences.

Current Technological Advances in Mucoadhesive Buccal Drug Delivery Systems and Their Therapeutic Applications

Buccal drug delivery has emerged as a promising route of administration, offering distinct advantages over conventional oral delivery systems. The unique physiological characteristics of the buccal mucosa, including rich vascularity and relatively high permeability, make it an attractive site for both local and systemic drug administration. Bioadhesive formulations have revolutionized buccal drug delivery by ensuring prolonged contact with the mucosal surface, thereby enhancing drug absorption and bioavailability. Recent developments in polymer science have introduced novel mucoadhesive materials, including thiolated polymers, lectins, and stimuli-responsive systems, which demonstrate superior adhesion properties and controlled release characteristics. Various formulation approaches, such as films, tablets, patches, and hydrogels, have been developed to optimize drug delivery through the buccal route. These systems incorporate permeation enhancers, enzyme inhibitors, and novel drug carriers to overcome the biological barriers associated with buccal delivery. The application of nanotechnology has further advanced the field, introducing nanostructured formulations that exhibit enhanced mucoadhesion and improved drug permeation. Current research focuses on developing intelligent delivery systems that respond to physiological triggers and maintain therapeutic drug levels. Understanding the critical factors affecting bioadhesion, including polymer characteristics, environmental conditions, and physiological variables, has led to the development of more effective formulations. The integration of modern analytical techniques and quality control measures ensures the development of stable and efficient buccal delivery systems and improves patient compliance and therapeutic outcomes

Compression optical coherence elastography as a new tool for non-invasive identification of precancerous lesions of the oral mucosa

Compression optical coherence elastography as a new tool for non-invasive identification of precancerous lesions of the oral mucosa

What is known about Peutz–Jeghers syndrome (a literature review)

Peutz–Jeghers syndrome (PJS) is an autosomal dominant disease, which in most cases is caused by mutation of the tumor suppressor gene STK11 and is characterized by the development of hamartomatous polyps in the digestive tract and the presence of hyperpigmented spots on the skin and mucous membranes. Late diagnosis of PJS leads to acute intestinal obstruction or bleeding. The aim of the study is to summarize the data and current views on the early diagnosis and treatment of PJS in children and processing information on monitoring of PJS complications. To achieve this goal, we have analyzed the sources of professional literature indexed in scientometric databases for the period 2014–2024. The analysis of the professional literature has shown that the triad is characteristic for PJS diagnosis: pigmented spots on the skin and oral mucosa, digestive tract polyposis, and heredity. The timing of symptom onset is an important aspect that requires further study of the clinical course and prognosis of the disease. Conclusions. Phenotypic suspicion involves the formation of risk groups using extraintestinal signs – pigmentation of the mucous membranes and lips – an early visual symptom that makes it possible to recognize PJS, prescribing timely examination and treatment long before the onset of complications. Dynamic observation and prevention of complications in risk groups – both in the presence of a family history and in newly diagnosed children with polyps and specific pigmentation should be provided. The main method of PJS diagnosis is endoscopic, and the main treatment is surgical.

A giant solitary Peutz-Jeghers-type polyp in the gastric body.

A 24-year-old young female presented to our hospital with gastrointestinal bleeding for four days. The patient had no family history of polyps or cancer. There were no pigment spots on her skin, lips, or oral mucosa. For diagnostic purposes gastroscopy and colonoscopy were all performed. Gastroscopy revealed a pedunculated polyp with a size of approximately 2.5 * 2.5cm in the gastric body. Colonoscopy showed normal. Then the polyp was excised by electrocoagulation with a snare. After the polyp was excised, the bleeding stopped. Histopathological analysis revealed that the polyp was a Peutz-Jeghers-type polyp (PJP) .

Periapical cysts in dogs: 10 cases (2000-2020).

To characterize the clinical, diagnostic imaging, and histologic features with description of treatment outcome of periapical cysts in dogs. Ten client-owned dogs diagnosed with periapical cysts biopsied between July 1, 2000 and June 30, 2020. Medical records of the Matthew J. Ryan Veterinary Hospital of the University of Pennsylvania were retrospectively searched to identify dogs that had surgical biopsy specimens of cavitary lesions diagnosed as odontogenic cysts and that met additional inclusion criteria. Complete medical records were reviewed. Range age and body weight of affected dogs were 2.5-12.1 years and 4.3-38.4 kg (9.5-84.7 lb), respectively. All periapical cysts were affecting the incisive bone and/or the maxilla. Nine dogs presented with a fluctuant swelling of the oral mucosa and gingiva; one dog without clinical swelling presented with a history of difficulty breathing. All cysts originated from a non-vital tooth with a structural defect (wear or fracture without pulp exposure) and/or intrinsic staining. Extraction of the associated non-vital teeth, enucleation of the cysts, and curettage of the surgical sites resulted in resolution of the clinical signs. The findings indicate that periapical cysts are associated with a non-vital tooth without pulp exposure. Complete evaluation of the clinical, diagnostic imaging, and histologic features of the lesion in affected dogs is necessary to differentiate periapical cysts from other odontogenic cysts and tumors.

Peutz–Jeghers syndrome – Be in need of vigilance: A case report

ABSTRACT Peutz–Jeghar syndrome (PJS) is an inherited condition that puts people at an increased risk for developing hamarotmatous polyps in the digestive tract as well as cancers of the breast, colon, rectum, pancreas, stomach, testicles, ovaries, lung and cervix. With typical presentation, majority cases of PJS can be diagnosed in childhood. PJS is inherited by mutation in the STK II gene, also known as LKB1 gene. We describe the case of a 14-year-old male who presented to us with recurrent abdominal pain, vomiting and weight loss associated with growth failure. Classic melanin spots were present on lips and buccal mucosa. Diagnosis of PJS was established via clinical history, examination, CT scan, and endoscopy revealing the need for laparotomy for bowel obstruction secondary to chronic intermittent intussusception, enterotomy for small polyps. Polyp histopathology was consistent with hamartomatous polyps of PJS.

Comparison of Salivary Opiorphin in Burning Mouth Syndrome and Healthy Subjects and Its Correlation With Psychiatric Disorders

ABSTRACTObjectivesBurning mouth syndrome (BMS) is a chronic disease in which the patient experiences a burning sensation in the oral mucosa without any pathological cause. Opiorphin is a pentapeptide that prevents pain and can have an antidepressant effect. The aim of this study was to evaluate salivary opiorphin concentration in BMS and healthy subjects. In addition, opiorphin concentration in BMS patients before and after psychiatric treatment was compared and the association between burning severity and psychiatric scores with salivary opiorphin concentration was examined.Materials and MethodsThis cross‐sectional study included 28 BMS patients and 40 healthy individuals referred to the Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Mashhad, from September 2018 to August 2019. Patients with proven disease based on clinical and psychiatric examinations were included in the study. Unstimulated salivary opiorphin levels were assessed by ELISA. Statistical analysis was performed using SPSS version 24.ResultsSalivary opiorphin levels were significantly higher in BMS patients (2.16 ± 0.30 ng/mL) than in healthy subjects (1.80 ± 0.36 ng/mL) (p < 0.001). Opiorphin levels in BMS patients increased insignificantly after psychiatric therapy (p = 0.212). In addition, there was no significant association between opiorphin levels and age, gender, menopause, burning severity, anxiety, and depression status.ConclusionsThe opiorphin concentration in saliva is higher in BMS patients than in healthy subjects. Most BMS patients also suffer from anxiety and depression.

Darya's disease: rare dermatosis with complicated manifestations

The pathogenesis of the disease is associated with mutations in the ATP2A2 gene of chromosome 12q23-24, which disrupt intracellular calcium metabolism. COVID-19 infection is known to cause a cytokine storm characterized by increased levels of tumor necrosis factor (TNF), interleukin (IL) 6 and 1β. TNF and IL-6 additionally reduce the activity of type 2 calcium ATPase in patients with Darye's disease. The typical clinical picture of rare hereditary dermatosis is characterized by hyperkeratotic papules located in seborrheic zones. Papules tend to fuse to form plaques. The surfaces of the palms and soles are also a frequent localization of the pathological process. Papules may grow into hypertrophic vegetations, damage to nails and oral mucosa, and the addition of secondary bacterial and viral infections. The typical course is of a continuous progressive nature with periods of exacerbation and incomplete remission. Systemic retinoids are the basis of therapy for Darye's disease. In addition, glucocorticosteroids and cyclosporine are systematically used. Local therapy includes keratolytic and antiseptic drugs, emollients. A case of clinical observation of a 39-year-old patient with Darye's disease is presented. The diagnosis was made based on the analysis of complaints, anamnesis, objective and local status, dermatoscopic picture, laboratory and histological examination. Against the background of ongoing therapy, the patient was discharged with clinical improvement and continues treatment on an outpatient basis. The clinical observation of this case is of interest to practicing dermatovenerologists.