Aluminum In Roots Research Articles

Genome variations account for different response to three mineral elements between Medicago truncatula ecotypes Jemalong A17 and R108.

BackgroundResequencing can be used to identify genome variations underpinning many morphological and physiological phenotypes. Legume model plant Medicago truncatula ecotypes Jemalong A17 (J. A17) and R108 differ in their responses to mineral toxicity of aluminum and sodium, and mineral deficiency of iron in growth medium. The difference may result from their genome variations, but no experimental evidence supports this hypothesis.ResultsA total of 12,750 structure variations, 135,045 short insertions/deletions and 764,154 single nucleotide polymorphisms were identified by resequencing the genome of R108. The suppressed expression of MtAACT that encodes a putative aluminum-induced citrate efflux transporter by deletion of partial sequence of the second intron may account for the less aluminum-induced citrate exudation and greater accumulation of aluminum in roots of R108 than in roots of J. A17, thus rendering R108 more sensitive to aluminum toxicity. The higher expression-level of MtZpt2-1 encoding a TFIIIA-related transcription factor in J. A17 than R108 under conditions of salt stress can be explained by the greater number of stress-responsive elements in its promoter sequence, thus conferring J. A17 more tolerant to salt stress than R108 plants by activating the expression of downstream stress-responsive genes. YSLs (Yellow Stripe-Likes) are involved in long-distance transport of iron in plants. We found that an YSL gene was deleted in the genome of R108 plants, thus rendering R108 less tolerance to iron deficiency than J. A17 plants.ConclusionsThe deletion or change in several genes may account for the different responses of M. truncatula ecotypes J. A17 and R108 to mineral toxicity of aluminum and sodium as well as iron deficiency. Uncovering genome variations by resequencing is an effective method to identify different traits between species/ecotypes that are genetically related. These findings demonstrate that analyses of genome variations by resequencing can shed important light on differences in responses of M. truncatula ecotypes to abiotic stress in general and mineral stress in particular.

Coordination between Apoplastic and Symplastic Detoxification Confers Plant Aluminum Resistance

Whether aluminum toxicity is an apoplastic or symplastic phenomenon is still a matter of debate. Here, we found that three auxin overproducing mutants, yucca, the recessive mutant superroot2, and superroot1 had increased aluminum sensitivity, while a transfer DNA insertion mutant, xyloglucan endotransglucosylase/hydrolases15 (xth15), showed enhanced aluminum resistance, accompanied by low endogenous indole-3-acetic acid levels, implying that auxin may be involved in plant responses to aluminum stress. We used yucca and xth15 mutants for further study. The two mutants accumulated similar total aluminum in roots and had significantly reduced cell wall aluminum and increased symplastic aluminum content relative to the wild-type ecotype Columbia, indicating that altered aluminum levels in the symplast or cell wall cannot fully explain the differential aluminum resistance of these two mutants. The expression of Al sensitive1 (ALS1), a gene that functions in aluminum redistribution between the cytoplasm and vacuole and contributes to symplastic aluminum detoxification, was less abundant in yucca and more abundant in xth15 than the wild type, consistent with possible ALS1 function conferring altered aluminum sensitivity in the two mutants. Consistent with the idea that xth15 can tolerate more symplastic aluminum because of possible ALS1 targeting to the vacuole, morin staining of yucca root tip sections showed more aluminum accumulation in the cytosol than in the wild type, and xth15 showed reduced morin staining of cytosolic aluminum, even though yucca and xth15 had similar overall symplastic aluminum content. Exogenous application of an active auxin analog, naphthylacetic acid, to the wild type mimicked the aluminum sensitivity and distribution phenotypes of yucca, verifying that auxin may regulate aluminum distribution in cells. Together, these data demonstrate that auxin negatively regulates aluminum tolerance through altering ALS1 expression and aluminum distribution within plant cells, and plants must coordinate exclusion and internal detoxification to reduce aluminum toxicity effectively.

No Statistic Correlation between Superoxide Dismutase and Peroxidase Activities and Aluminum-Induced Lipid Peroxidation in Maize, Implying Limited Roles of Both Enzymes in Prevention against Aluminum-Induced Lipid Peroxidation

Changes of and correlation among root tolerance index (RTI), root Aluminum (Al) content, root/shoot ratio (RSR), root malondialdehyde (MDA) content, and Superoxide dismutase (SOD) and peroxidase (POD) isoforms of maize YQ 7-96 were investigated under Al stress and removal of the stress (RS). Consequently, Al stress led to significant decreases in RTI, RSR, SOD and POD activities, but resulted in significant increase in root MD A and, Al accumulation in the tissues; Root SOD and POD activities did not correlate with Al and MDA contents in roots; The activities of SOD and POD were much lower in roots than in leaves. It can be concluded that (1) Al stress can lead to lipid peroxidation; (2) there is a larger POD family composed of different POD isoforms, some of which are of tissue-specific expression and play different roles in detoxification of Al in maize; (3) for POD isoforms, POD 2 is root-specific. POD 6 and POD 7 are all leaf-specific, POD 5 is not only root-specific but also RS-responsive; (4) high sensitivity of maize to Al is in part associated with much lower activities of both SOD and POD in roots; and (5) more importantly, both SOD and POD are therefore hinted to be not key players in prevention against Al-induced lipid peroxidation.

Distribution and Chemical Forms of Aluminum in Roots of Aluminum-Tolerant Rice Cultivar Grown with or without Phosphate Pretreatment

The distribution and chemical forms of aluminum (Al) were determined in the roots of the Al-tolerant rice cultivar Arkansas cultured in the presence of Al after being precultured with or without phosphates. Lumogallion staining was used to locate Al, and the highest levels of Al were found in the root cap of the plants precultured with phosphates. High levels of Al were also seen in the apoplasmic areas around epidermal and stelar cells of the primary root, and in the area around stelar cells of the lateral roots. Omission of the phosphate preculture reduced Al levels in the root cap and the areas around the epidermal cells. Chemical speciation of the Al that precipitated in the roots was determined by 27Al magic angle spinning NMR spectroscopy. The spectra demonstrated that the precipitate contained significant levels of Al hydroxides, with Al(OH)2H2PO4 as a minor component. These results suggest that the formation of an Al deposit is the main mechanism of Al tolerance in this plant. Additional Al tolerance may result from the increased Al deposition in the root cap and epidermal apoplasmic area in phosphate-pretreated plants. The formation of Al(OH)2H2PO4 may account for the increased Al deposition.

The presence of aluminum in arbuscular mycorrhizas of Clusia multiflora exposed to increased acidity

In this work, we present the results obtained after 9 months of watering with acidic solutions seedlings of Clusia multiflora, inoculated with arbuscular mycorrhizal fungi (AMF). The fungi were isolated from acid and neutral soil. C.multiflora is a tropical woody species that naturally grows on acid soils high in soluble Al. The research evaluated if arbuscular mycorrhizas (AM) could be responsible at least partially for the tolerance to acidity and to aluminum of C.multiflora and if an inoculum of AM fungi (AMF) coming from acid soils contributes more to the tolerance of acidity of C. multiflora than one coming from neutral soils. Results showed that in the absence of AMF (control treatment), the seedlings of C. multiflora did not grow, indicating that this species is highly dependent on AMF. When C. multiflora was exposed to a very acidic solution (pH 3), plants inoculated with AMF from acid soils were taller than those inoculated with AMF from neutral soils. Acidity affected root growth and root length. Plants inoculated with AMF from neutral soils showed thicker roots and lower shoot-root relationships than those inoculated with AMF from acid soils. Acidity did not affect root growth of C. multiflora inoculated with AMF from acid soils even when they were watered with solutions of pH 3. All plants accumulated high quantities of Al in roots (>10 000 mg.kg −1), but plants inoculated with AMF from acid soils, accumulated less aluminum in roots than plants from the other treatments. A histochemical study of the distribution of Al in roots showed that in mycorrhizal plants, the aluminum was bound to the cell walls in the mycelium of the fungus, mainly in the vesicles or in auxiliary cells, a fact showed for the first time in this work.

Impacts of (NH4)2SO4 deposition on Norway spruce (Picea abies [L.] Karst) roots

The effects of enhanced (NH4)2SO4 (NS) deposition on Norway spruce (Picea abies [L.] Karst) fine root biomass, vitality and chemistry were investigated using root-free in-growth cores reproducing native organic and mineral soil horizons. The cores were covered and watered every 2 weeks with native throughfall or throughfall supplemented with NS to increase deposition by 75 kg ha-1 a-1 NH4+-N (86 kg ha-1 a-1 SO42--S). The in-growth cores were sampled after 19 months and assessed for root biomass, necromass, length, tip number, tip vitality and fine root chemistry. Root biomass and fine root aluminium (Al) concentration were negatively correlated, but NS deposition had no effect on root growth or root tip vitality. NS deposition caused increased fine root nitrogen (N) concentrations in the organic horizon and increased Calcium (Ca) concentrations in the mineral horizon. Fine root biomass was higher in the organic horizon, where fine root Al and potassium (K) concentrations were lower and Ca concentrations higher than in the mineral horizon. Results highlighted the importance of soil stratification on fine root growth and chemical composition.

Uptake and distribution of calcium and phosphorus in beech (Fagus sylvatica) as influenced by aluminum and nitrogen.

We studied the effects of excess nitrogen added as nitrate (NO(3) (-)) or ammonium (NH(4) (+)), or both, on mineral nutrition and growth of beech (Fagus sylvatica L.) plants grown at pH 4.2 in Al-free nutrient solution or in solutions containing 0.1 or 1.0 mM AlCl(3). A high external concentration of NH(4) (+) increased the concentration of nitrogen in roots, stems and leaves. The root/shoot dry weight ratio was less in plants grown in the presence of NH(4) (+) than in plants grown in the presence of NO(3) (-). The concentration of phosphorus in the roots was increased and the concentration of potassium in all parts of the plant was decreased by NH(4) (+). A high external concentration of NO(3) (-) caused a decrease in phosphorus concentrations of the root, stem and leaf. Uptake of (45)Ca(2+) by roots was reduced in the presence of high concentrations of NH(4) (+) or NO(3) (-), and a combination of high concentrations of nitrogen and aluminum further reduced the uptake of (45)Ca(2+). Uptake of phosphate ((32)P) and concentrations of phosphorus in root and shoot were increased when plants were grown in the presence of 0.1 mM Al. Exposure to 1.0 mM Al, however, reduced the concentration of phosphorus in roots and shoots and the reduction was greater when plants were grown in the presence of a high external NO(3) (-) concentration. Aluminum binds to roots, and plants grown in the presence of 1.0 mM Al had a slightly higher concentration of aluminum in roots than plants grown in the presence of 0.1 mM Al, whereas the concentration of Al in the shoot was increased 2 to 3 times in plants exposed to 1.0 mm Al. Furthermore, the effects of 1.0 mM Al on uptake of other macronutrients were quite different from the effects of 0.1 mM Al. We conclude that 0.1 mM Al facilitates uptake and transport of phosphorus in beech and that between 0.1 and 1.0 mM Al there is a dramatic change in the effects of Al on uptake and transport of divalent cations and phosphorus.