Alternative Behaviors Research Articles

Evaluation of resurgence following differential reinforcement of alternative behavior with and without extinction in a human operant model.

One of the most common treatments for severe challenging behavior involves placing the challenging behavior on extinction and differentially reinforcing an alternative response (DRA). However, extinction is not always feasible and may be unsafe or impractical to implement in some circumstances. Thus, implementing a DRA without extinction intervention may be necessary for some cases. Currently, the extent to which DRA without extinction produces durable treatment outcomes, particularly as it relates to the resurgence of challenging behavior, is unclear. The present study investigated resurgence following DRA with and without extinction using a three-phase resurgence evaluation in a translational human operant model with college students as participants. All participants demonstrated resurgence across both experimental groups. Although there were no statistically significant differences in the prevalence, magnitude, or persistence of resurgence between groups, levels of resurgence magnitude were relatively higher in the DRA-without-extinction group than in the DRA-with-extinction group. Clinical implications of these findings and directions for future human operant investigations of resurgence are discussed.

Implementing a Positive Behavior Support Program for Young Children with Autism in Public Agencies: A Social Validity Evaluation from Parents, Practitioners, and Administrators.

Challenging behaviors in young children with autism and intellectual disabilities pose significant barriers to learning and inclusion. The Prevent-Teach-Reinforce for Young Children (PTR-YC) program is an evidence-based intervention that addresses these behaviors according to Positive Behavior Intervention Support principles. It is essential to assess the social validity of an intervention, when implemented into a public service system, to ensure sustainability and relevance to stakeholders. The present study aimed to document the social validity of PTR-YC from the point of view of three key informants: parents, practitioners, and administrators. Sixty-one participants were interviewed about their experience with PTR-YC (9 administrators, 31 practitioners, and 20 families). Semi-structured interviews assessed three fundamental components of social validity (Wolf, 1978): (a) social relevance of intervention goals, (b) social appropriateness of intervention procedures, and (c) social importance of intervention effects. Participants' responses were subjected to thematic analysis. Intervention goals, namely family participation in the clinical process, strategies that focuses on prevention, and teaching alternative adaptive behaviors, were identified as relevant. Participants appreciated the program's clear, structured, and turnkey clinical process, its parent-practitioner collaboration, and its consistency with practices already in place in the field. Positive instrumental outcomes (e.g., knowledge, competencies, self-efficacy) and ultimate outcomes (e.g., decrease in challenging behavior) of the intervention were reported on children, families, and practitioners. This study represents the first in-depth evaluation of the social validity of PTR-YC in specialized public services for autism and demonstrates its appropriateness for universal implementation in this sector.

Sugar feeding in triatomines: a new perspective for controlling the transmission of Chagas disease.

Introduction: Triatomines are vectors of Trypanosoma cruzi, the etiological agent of Chagas disease. Currently, there is no vaccine against this disease. Thus, control of the insect vector population is the main strategy available to reduce the number of cases. Triatomines are considered obligate hematophagous, but different alternative feeding behaviors were described, such as haemolymphagy or plant feeding. Methods: To determine the preference for sugar feeding in nymphs and adults of Rhodnius prolixus, the insects were exposed a piece of cotton containing bromophenol blue plus sucrose. In addition, we offered several sugars for different species of triatomines, and tested sugar meals as a route of delivery of insecticides in first-instar nymphs of R. prolixus. The effect of sugar feeding on the physiology of these different species of triatomines was recorded. Results: First instar nymphs ingested sucrose more strongly than other stages, and showed high mortality rates. In different species of triatomines, sucrose induced an ingestion, but engorgement varied according to the species. R. prolixus nymphs showed an indiscriminate intake of various sugars, with very different physiological effects. Furthermore, ingesting different combinations of insecticides + sugar significantly reduced insect survival. Discussion: In summary, we described for the first-time sugar feeding as a widespread behavior in several species of triatomines, and the possibility of the use of toxic sugar baits for the control of these vectors. The knowledge of feeding behavior in these insects can be fundamental for the development of new strategies to control Chagas disease.

One health: the impact of environment, detergents and hygiene on barrier, microbiome and allergy

SummaryA variety of body surfaces, such as skin and mucosal membranes—from the nasopharyngeal area to the lungs, uterus, vaginal area, and digestive tract—contain complex microbial ecosystems that are tailored to the specifics of the respective niche [1].The so-called dysbiosis—a disadvantageous change in the composition of the microbiome—is associated with the pathogenesis of a variety of diseases [2]. Gastrointestinal as well as cardiovascular, metabolic, neurodegenerative, psychological, oncological, and also allergic diseases have been linked to microbial dysbiosis. Susceptibility to allergies can be due to genetic predisposition; in addition, extrinsic factors from today’s lifestyle increasingly contribute to microbiome changes, but also to the disruption of the skin and mucosal barrier and thus to the development of allergies [3].Gisela, a fictional farmer, guides us through this review. She is representative of adults and children of all genders in industrialized countries. During her daily routine, the skin and mucosal microbiome is influenced by a variety of exogenous factors. These include everyday personal hygiene products, detergents for laundry and dishes, food, medication, animal contact, and exposure to various outdoor environments. Gisela’s daily routine will illustrate how the human microbiome and the skin barrier are modified in positive or negative ways, and how this could influence the development of allergies. Furthermore, potential measures for the prevention and management of dysbiosis will be discussed in terms of examples of alternative products and behaviors.

Alzheimer's dementia in people with Down syndrome : Results of guideline-assisted expert interviews on healthcare deficits in the diagnostics and treatment as well as solution approaches

People with Down syndrome have agenetically increased risk of developing early onset Alzheimer's dementia. An interview study with healthcare providers, patient representatives and employees in residential and work facilities was conducted to identify deficits in the healthcare process and approaches to overcoming them. In this study 14 semi-structured interviews were conducted and analyzed using qualitative content analysis. Alack of knowledge and experience on the part of medical service providers in dealing with and providing medical care for people with Down syndrome was identified as akey challenge. In addition, the diagnosis of dementia in people with Down syndrome is difficult for various reasons (including lack of appropriate diagnostic tools in standard care and lack of time or financial resources). Doubts were expressed about the efficacy of antidementia medications and the reasons for the increased use of sedatives were discussed. Attentive observation of behavior and involvement of caregivers, regular review and reduction of polypharmacy and the use of alternative behavior modification techniques were mentioned as possible solutions. The identified deficits in the medical care of the target population and the approaches to solving them will be incorporated into the development of health policy recommendations in order to optimize the care situation of those affected in the long term.

Stem extract from Costus afer (Bush cane) prevents ethanol-induced neuronal degeneration in mice via an antioxidant-inflammatory pathway

BackgroundExcessive alcohol consumption is associated with memory impairment and can lead to Alzheimer's disease-like dementia, contributing to the rising prevalence of dementia worldwide. Costus afer (Bush cane) is a medicinal plant widely used in traditional African medicine for its anti-inflammatory, antioxidant, and neuroprotective properties. It is commonly employed to treat various ailments, including inflammatory diseases, respiratory conditions, and gastrointestinal disorders. PurposeGiven its traditional use in the treatment of kyphosis (hunchback) which is implicated in cerebral palsy and degenerative disease of the spine, we explored its potential neuroprotective effects against ethanol-induced neuronal degeneration, in this research, we examined the potential protective impacts on memory and neurology afforded by the aqueous stem extract derived from C. afer in mice subjected to ethanol exposure. MethodsForty-five male Swiss mice weighing 22–35 g were divided into five groups (n = 9): control (treated with distilled water orally), memory-impaired (treated with ethanol at 5 g/kg orally), and three groups treated with ethanol followed by administration of C. afer at doses of 100 mg/kg, 200 mg/kg, and 500 mg/kg respectively. All animals were provided unrestricted access to both food and water throughout the duration of the study. Memory impairment was assessed after 28 days using the T-maze spontaneous alternation test. Subsequently, spectrophotometry, ELISA, and histomorphometry were employed to evaluate indicators of neuronal inflammation, oxidative stress linked to inflammation, and degenerative changes were assessed in the hippocampus, prefrontal cortex, and cerebellum. ResultsEthanol administration led to a notable (p < 0.01) decline in neurobehavioral function, as evidenced by reduced spontaneous alternation behavior, which was mitigated by C. afer treatment, leading to increased percentage alternation. Furthermore, ethanol administration altered endogenous antioxidant levels and pro-inflammatory mediators, resulting in elevated lipid peroxidation, nitrite, tumor necrosis factor-alpha, interleukin-6, and decreased superoxide dismutase activity, promoting neuronal degeneration in the mice brains. However, treatment with C. afer at doses of 100 mg/kg, 200 mg/kg, and 500 mg/kg substantially (p < 0.05) attenuated oxidoinflammatory stress by reducing levels of MDA, NO-2, TNF-α, IL-6, while upregulating SOD activity, thereby preserving neuronal integrity in the hippocampus, prefrontal cortex, and cerebellum. ConclusionThese findings suggest that C. afer mitigates the progression of memory impairment induced by ethanol through mechanisms involving the suppression of oxidoinflammatory stress facilitators and inhibition of cortico-hippocampal and cerebellar neuronal degeneration in murine subjects.

12539 Estrogen Type In Contraceptives Matters: Effects On Spatial Memory And Anxiety-Like Behaviors In A Female Rat Model

Abstract Disclosure: A.P. Hegwood: None. S. Kujawa: None. A.V. Prakapenka: None. According to U.S. Centers for Disease Control &amp; Prevention, ∼12% of the U.S. population uses combined oral contraceptives (COCs). Behavioral side effects are common factors in COC discontinuation. Management of COC-related side effects primarily centers around altering the synthetic ethinyl estradiol (EE) dose, the predominant estrogen in COCs. Estradiol valerate (EV) is a recently introduced natural estrogen alternative to EE. EE-containing COCs, such as EE/dienogest (DNG), were found to decrease free steroid hormone serum levels compared to EV-containing COCs, such as EV/DNG. In women and in female rodent models, steroid hormones (e.g., 17-ß estradiol (E2), testosterone) dose-dependently modulate spatial memory and anxiety-related behaviors. We hypothesized that in a rat model of hormonal contraceptive use, EE/DNG differentially modulates spatial memory and anxiety-like behaviors compared to EV/DNG, corresponding to circulating steroid hormone levels. Thirty-six young-adult (3-4-months-old) female rats were randomly assigned to EE/DNG (0.13µg EE and 8.6µg DNG per day), EV/DNG (8.6µg EV and 8.6µg DNG per day), or vehicle treatment (propylene glycol) continuously administered by subcutaneous osmotic pump. At four weeks of treatment, rats were tested on the delayed spontaneous alternation (dSA) task to evaluate spatial memory and the elevated plus maze (EPM) to evaluate anxiety-like behavior. Following EPM, serum was collected and analyzed for E2, progesterone, androstenedione, and testosterone levels using liquid chromatography-mass spectrometry and corticosterone levels using radioimmunoassay. Spontaneous alternation behaviors were similar across all three groups on the dSA. However, EE/DNG increased use of an egocentric navigational strategy, the use of consecutive turns, on the dSA compared to vehicle and EV/DNG. Egocentric strategy is likely mediated by the striatal memory system, engagement of which is elevated with stress and anxiety. Of note, EE/DNG decreased time spent in the open arms of the EPM compared to vehicle and EV/DNG, indicating increased anxiety-like behaviors with EE/DNG. Both EE/DNG and EV/DNG decreased androstenedione, testosterone, and E2 circulating levels compared to vehicle. Steroid hormone serum levels did not differ between EE/DNG and EV/DNG; 58% of EE/DNG serum samples were undetermined/below threshold for E2 levels, limiting statistical analysis. Altogether, we found that, compared to vehicle and the natural EV, the synthetic EE component in hormonal contraceptives elevates anxiety-like behaviors in female rats, an effect that corresponds to increased use of an egocentric navigational strategy on a spatial memory task. Our findings support the notion that estrogen type matters for behavioral outcomes associated with contraceptive use, identifying estrogen type as a possible tool for management of COC-related side effects. Presentation: 6/1/2024

Assessment of reduction in stimulus generalization of ethanol-seeking during recovery: A rapid procedure

Previously, we developed a procedure which showed that longer histories of reinforced alternative behavior decrease the risk of relapse caused by a range of stimuli which had previously occasioned drinking. The decrease in relapse risk was likely due to a decrease in attention to the stimuli over the course of repeated engagement in the alternative behavior. However, this previous procedure was time consuming and did not mirror the procedure we used to observe changes in relapse risk. This study aimed at replicating the previous relationship between the duration of engaging in an alternative behavior and shift in stimulus generalization for drinking using a procedure that allows longitudinal analysis over time and is consistent with other procedures we have developed. Rats were trained to respond for ethanol in the presence of one stimulus (16 kHz tone; food Fixed Ratio (FR)150 and ethanol FR5), and for food in the under another stimulus (8 kHz tone; food and ethanol FR5). Then, recovery-like sessions with food predominant responding occurred in the presence of only the low-cost food stimulus. During these sessions, rats were exposed to non-reinforced graded stimuli alternation from 8 to 16 kHz alternating with the reinforced low-cost food stimulus. The number of responses on each (food and ethanol) lever before completing 5 responses on either lever was the main measure. Consistent with the earlier procedure, the current procedure showed that graded variation of tone from 8 to 16 kHz produced a graded increase in responding for ethanol compared to responding for food. In addition, longer periods of engaging in recovery-like responding shift the generalization function downwards. This procedure confirms the earlier pattern of stimulus generalization over longer periods of behavior consistent with recovery. This strengthens our hypothesis that shifts in attention to alcohol-related stimuli are important to the development of relapse resistance during recovery.

A hypothetical model behind the molecular mechanism of alternate behaviour in Pistacia vera L.

A hypothetical model behind the molecular mechanism of alternate behaviour in <i>Pistacia vera</i> L.

Design and synthesis of phyto-Saxagliptin conjugates: Targeting DPP IV, AChE, and β-amyloid in cognitive impairment

Cognitive impairment is a major secondary consequence of Type II diabetes mellitus. The present investigation is designed to enhance the efficacy of an antidiabetic drug (Saxagliptin/SXG) against diabetes-induced cognitive impairment in rats. SXG has limited blood-brain barrier (BBB) permeability due to its hydrophilic nature. Phytochemical-based carboxylic acid conjugates enhance the lipophilicity and anti-oxidant properties of the drugs. Virtually SXG was conjugated with 10 different carboxylic acids and evaluated for drug-likeness, pre-ADMET, Molecular docking, and HUMO-LUMO study. Based on the molecular weight, log P value, docking score, and toxicity profile; three carboxylic acids were selected. Gallic acid (GA), Isonicotinic acid (INA), and Caffeic acid (CA) conjugates of SXG were synthesized and characterized by FTIR, and NMR studies. Diabetes-induced cognitive impairment was induced in the rats by administration of STZ, 45 mg kg-1 i.p. Learning and memory were evaluated by assessing spontaneous alternation behavior (SAB), number of correct responses, and transfer latency using Y-maze, radial arm maze, and elevated plus maze respectively. The antioxidant activity, nitrite levels, acetylcholine esterase level, and Aβ42 content in the rat brain was also evaluated. Histopathology of the hippocampal area was also done. The carboxamides of SXG have improved the efficacy of SXG against diabetes-induced cognitive impairment. This neuroprotective effect of carboxamides of SXG may be attributed to anti-oxidant activity, cholinergic activity, and anti-amyloid β activity.

What determines the antipredator strategy in antlion larvae? Burrowing ability decreases the duration of post‐contact immobility

Abstract For prey exhibiting alternative antipredator strategies, selecting the most effective one is critical for survival. However, what determines such selection remains an open question. We hypothesised that this selection depends on prey skill. We test this idea in Myrmeleon immaculatus De Geer, 1773 (Neuroptera: Myrmeleontidae) antlion larvae that exhibit two alternative antipredator behaviours: post‐contact immobility (PCI) and burying. As the larvae live under sandy soils, PCI is only a temporary strategy, and eventually, all larvae will burrow. However, deciding how long to remain immobile before burying may depend on how fast larvae bury themselves (i.e., how quickly the individual can camouflage itself in the substrate). In a tropical forest from the Yucatan peninsula, Mexico, we measured the PCI duration, bury time and body size of 45 larvae in a fine‐grained experimental substrate. We also measured the mandible length to assess their effects on burying skills. The PCI duration was consistently shorter in larvae that took less time to bury but was longer and more variable in larvae that took more time to bury. This effect was independent of larvae body size. Mandible length did not affect burial time. Our findings suggest that when a given prey should select between two mutually excluding antipredator strategies, the intrinsic skill to perform the most relevant one (how fast burying themselves, in this case) may modulate the duration of the other (PCI, in this case). This reinforces the role of predation as a selective force that modulates prey behaviours.

Monitoring change: A Behavior‐Centered Theory of Change for effective demand reduction interventions

AbstractBehavior change campaigns are crucial in combatting illegal wildlife trade (IWT) by reducing demand, but assessing their impact requires robust strategies. The Behavior‐Centered Theory of Change (BC‐ToC) integrates Theory of Change frameworks with decision‐making models to design and evaluate interventions using behavioral and progress indicators. We used BC‐ToC to design two behavioral change interventions aimed at reducing demand for two threatened Venezuelan bird species: the Yellow‐shouldered Amazon (YSA) and the Red Siskin (RS). We developed workshops to engage community organizations and conservationists to identify audience groups, evaluate alternative behaviors considering impact and benefits, and define behavior‐based indicators for monitoring change. For YSA, two audience groups with similar demand motivations emerged: “common folks” (women, 20–70 yo, low education) and “young professionals” (20–30 yo, educated). Enjoying parrots through outdoor activities emerged as an alternative. For RS, the main audience segments were the breeder groups “South American node” (Venezuelan and Brazilian) and “Iberian node” (Spaniels and Portuguese). Iberian with wider age range (30–60 yo) and greater education than South American. Adopting responsible sourcing practices surfaced as the alternative behavior. We described behavioral levers, intermediary outputs, and indicators reflecting changes in knowledge, attitudes, norms, and control. We discussed challenges for adoption, emphasizing systemic barriers and the role of regulations, and provided ground‐tailored strategies for effective behavioral interventions.

SMSs as an alternative to provider-delivered care for unhealthy alcohol use: study protocol for Leseli, an open-label randomised controlled trial of mhGAP-Remote vs mhGAP-Standard in Lesotho

BackgroundThe World Health Organization’s (WHO) Mental Health Gap Action Programme (mhGAP) is a validated intervention that can be provided by non-specialised healthcare workers to individuals with unhealthy alcohol use. However, it typically requires several in-person sessions at a health facility, which may limit its feasibility and effectiveness in remote settings. This trial compares mhGAP-Standard, a 4 to 6 in-person session intervention, to mhGAP-Remote, a 1 in-person session intervention followed by 8 week of short message service (SMS) in Lesotho. We hypothesise that mhGAP-Remote is superior to mhGAP-Standard in reducing alcohol use (as detailed by the primary and secondary outcomes below).MethodsThis is a two-arm randomised open-label multicentre superiority trial. Participants allocated to mhGAP-Standard receive 4 in-person sessions using motivational interviewing, identifying triggers, and alternative behaviours, with the option of two additional booster sessions. Participants in the mhGAP-Remote arm receive 1 in-person session covering the same content, followed by standardised SMSs over 8 weeks that reinforce intervention content. Non-specialist providers deliver the intervention and receive weekly supervision. Adults (Nplanned = 248) attending participating health facilities for any reason and who meet criteria for unhealthy alcohol use based on the Alcohol Use Disorders Identification Test ([AUDIT] score ≥ 6 for women, ≥ 8 for men) are individually randomised to the two arms (1:1 allocation, stratified by participant sex and age (≥ 50 vs < 50 years old). Follow-up assessments occur at 8, 20, and 32 weeks post-randomisation. The primary outcome is change in self-reported alcohol use (continuous AUDIT score), from baseline to 8 weeks follow-up. Change in the AUDIT from baseline to 20 and 32 weeks follow-up is a secondary outcome. Change in the biomarker phosphatidylethanol (secondary), liver enzyme values in serum (exploratory), and HIV viral load (for people with HIV only; exploratory) are also evaluated from baseline throughout the entire follow-up period. A linear regression model will be conducted for the primary analysis, adjusted for the stratification factors. Three a priori sensitivity analyses for the primary outcome are planned based on per protocol treatment attendance, recovery from unhealthy alcohol use, and clinically significant and reliable change.DiscussionThis trial will provide insight into feasibility and effectiveness of a shortened and primarily SMS supported version of mhGAP, which is especially relevant for settings where regular clinic attendance is a major barrier.Trial registrationclinicaltrials.gov NCT05925270. Approved on June 29th, 2023.

Faraday instability of viscous liquid films on a heated substrate with Maxwell–Cattaneo heat flux

Faraday instability of viscous liquid films with Maxwell–Cattaneo (MC) heat flux on an infinite, heated horizontal substrate subject to vertical time-varying periodic vibration is investigated theoretically. The MC effect means that the response of the heat flux to a temperature gradient obeys a relaxation time law rather than a classical Fourier time law. Applying the classic Floquet theory to linear analysis, a recursive relation is obtained. When considering the MC effect, a new phenomenon appears at a large wave number k. The neutral stability curves branch new tongues that turn left rather than right as before, but the tongues still move up and right as the wave number increases. Furthermore, typical harmonic (H) and subharmonic (SH) alternation behavior continues to exist. Interestingly, the first tongue of a branch is H or SH, implying that there is a transition following the branches. However, near the critical wave number kc of a branch, the SH and H almost overlap. As Cattaneo number C increases, the tongue-like unstable zones of branches become wider, and the critical wave number kc of the appeared branch becomes small. As the driving frequency ω decreases, the branch tongues become elongated and the critical wave number kc of the appeared branch becomes small.

FAM19A5 Deficiency Mitigates the Aβ Plaque Burden and Improves Cognition in Mouse Models of Alzheimer's Disease.

FAM19A5, a novel secretory protein highly expressed in the brain, is potentially associated with the progression of Alzheimer's disease (AD). However, its role in the AD pathogenesis remains unclear. Here, we investigated the potential function of FAM19A5 in the context of AD. We generated APP/PS1 mice with partial FAM19A5 deficiency, termed APP/PS1/FAM19A5+/LacZ mice. Compared with control APP/PS1 mice, APP/PS1/FAM19A5+/LacZ mice exhibited significantly lower Aβ plaque density and prolonged the lifespan of the APP/PS1 mice. To further explore the therapeutic potential of targeting FAM19A5, we developed a FAM19A5 antibody. Administration of this antibody to APP/PS1 mice significantly improved their performance in the Y-maze and passive avoidance tests, indicating enhanced cognitive function. This effect was replicated in 5XFAD mice, a model of early-onset AD characterized by rapid Aβ accumulation. Additionally, FAM19A5 antibody treatment in 5XFAD mice led to enhanced exploration of novel objects and increased spontaneous alternation behavior in the novel object recognition and Y-maze tests, respectively, indicating improved cognitive function. These findings suggest that FAM19A5 plays a significant role in AD pathology and that targeting with FAM19A5 antibodies may be a promising therapeutic strategy for AD.

Travel mode choice tolerance (TMC tolerance): Exploring elementary school students' perceived ability to switch commuting modes

BackgroundAs an essential outdoor activity for children, the school commute has received repeated attention in the existing literature. Nevertheless, prior studies on the travel mode choice for school commutes have predominantly focused on the preferred commuting mode, overlooking the exploration of students' travel mode alternative behaviors. In this context, research is crucial to capture students' propensity and perceived ability to shift their travel mode from car to public and active transport. MethodsTo address this gap, our study introduces a definition of ‘Travel Mode Choice tolerance’ (TMC tolerance) to explore alternative mode behaviors during school commutes for elementary school students. The latest data from the Beijing Student Commuting Survey are adopted to analyze student populations with varying TMC tolerance levels. The chi-squared test is also employed to examine the impact of different influencing factors on TMC tolerance. ResultsBased on the empirical results derived from this definition, it is observed that students with a strong tolerance for modal shifts constitute more than half of the student population. Factors such as grade level, mode of escort, and distance were identified as significant influencers on children's TMC tolerances. Particularly noteworthy is that the percentage of students with a strong TMC tolerance significantly increases when the travel distance exceeds 2.25 km. Among students traveling alone, a higher proportion exhibits low tolerance than those with high tolerance. Additionally, the proportion of students with strong TMC tolerance increases with grade level (age). ConclusionsOperationalizing TMC tolerance as preference and ability variation can capture the collective impact of complex factors shaping commuting decisions, both observed and unobserved. According to the findings, four policy suggestions are proposed, including optimizing school site selection, establishing school buses or children-concerned transit systems, enhancing healthy travel education, and planning active travel routes.

Microstructure of the dentate gyrus and spontaneous alternation behaviour of male Wistar rats following Rauvolfia vomitoria and Gongronema latifolium extracts administration

Rauvolfia vomitoria (RV) and Gongronema latifolium (GL) are medicinal plants used for the local treatment of various health issues. Their activities on the brain motivated this investigation on the histology and immunohistochemistry of the dentate gyrus and spontaneous alternation behaviour (SAB) of adult Wistar rats following RV root bark and GL leaf extract administrations. Twenty young adult Wistar rats (130–160 g) were assigned into four groups: Group 1 served as the control (5 mL/kg of distilled water placebo), while the test groups 2–4 were, respectively, singly administered 200 of mg/kg of RV, 200 of mg/kg of GL, and their combination. The administrations were oral and lasted for seven days. A T-maze SAB test was carried out, and the animals were sacrificed immediately after ketamine hydrochloride intraperitoneal anaesthesia. Serial sections of the hippocampal region from perfused rat brains were stained with Cresyl fast violet and immunolabelled with neuronal nuclei (NeuN) for neurons and glial fibrillary acidic protein (GFAP) for astrocytes. Results indicated that SAB was significantly (p &lt; 0.05) lower in the test groups. Histologically, Nissl was less distributed in the RV and GL-only groups but not in the combined group, while there was less NeuN positivity in the RV group, with the GL and RV + GL groups not affected. There was less positive GFAP expression in individual RV and GL groups, but not in the RV + GL combined group, all compared with the control. In conclusion, the combination of RV and GL did not improve SAB but modulated Nissl, NeuN, and GFAP expression in the dentate gyrus.

Resurgence Mitigation Across Extended Extinction Following Four and Eight Cycles of On/Off Alternative Reinforcement

Resurgence is an increase in an extinguished operant response resulting from a worsening of conditions (e.g., extinction) for a more recently reinforced alternative behavior. Previous research has shown that exposure to cycles of alternative reinforcement available versus unavailable (i.e., on/off alternative reinforcement) across sessions can reduce subsequent resurgence. Most previous assessments of the procedure have examined target operant responding during only single-session resurgence tests, and it remains unclear if exposure to relatively few cycles of on/off alternative reinforcement can maintain low rates of target behavior across extended exposure to extinction. This experiment with rats examined the effects of 4 or 8 cycles of on/off alternative reinforcement on subsequent resurgence during a 10-session extinction test. The results show that exposure to 4 cycles of on/off alternative reinforcement is as effective as 8 cycles in producing low rates of target behavior during treatment and across extended extinction. This result is consistent with extant theories of resurgence and suggests that on/off alternative reinforcement could have translational utility following relatively few cycles of exposure.

Synthesized alternative reinforcement and resurgence.

In treatments based on differential reinforcement of alternative behavior, applied researchers and clinicians often provide multiple, qualitatively different reinforcers (i.e., synthesized reinforcement) rather than a single reinforcer (i.e., isolated reinforcement) contingent on alternative behavior. Some research shows that providing synthesized reinforcement for alternative responses within such treatments produces more rapid and complete suppression of target behavior; however, there is limited research evaluating the durability of these effects during treatment disruptions. Conceptual explanations of resurgence (e.g., resurgence as choice, context theory) suggest that treatments that include synthesized alternative reinforcement may lead to more resurgence of target behavior when alternative reinforcement is disrupted relative to treatments using isolated reinforcement. We evaluated this hypothesis within a three-phase resurgence evaluation. We exposed rats to isolated or synthesized reinforcement for alternative responding in the second phase, and we exposed rats to extinction in the third phase. Synthesized alternative reinforcement produced more rapid and complete suppression of target behavior than did isolated reinforcement in the second phase; however, exposure to extinction following synthesized reinforcement produced more resurgence. We discuss these results in terms of their implications for applied research and their support for current conceptual explanations for resurgence.

Alleviative effect of scopolamine‑induced memory deficit via enhancing antioxidant and cholinergic function in rats by pinostrobin from Boesenbergia rotunda (L.).

Pinostrobin, a key bioactive compound found in the medicinal plant Boesenbergia rotunda (L.), has been noted for its beneficial biological properties including antioxidant, anti-inflammation, anti-cancer and anti-amnesia activities. In view of this, the present study purposed to evaluate the neuroprotective potential of pinostrobin in reversing scopolamine-induced cognitive impairment involving oxidative stress and cholinergic function in rats. A total of 30 male Wistar rats were randomly divided into five groups (n=6): Group 1 received vehicle as a control, group 2 received vehicle + scopolamine (3 mg/kg, i.p.), group 3 received pinostrobin (20 mg/kg, p.o.) + scopolamine, group 4 received pinostrobin (40 mg/kg, p.o.) + scopolamine and group 5 received donepezil (5 mg/kg, p.o.) + scopolamine. Treatments were administered orally to the rats for 14 days. During the final 7 days of treatment, a daily injection of scopolamine was administered. Scopolamine impaired learning and memory performance, as measured by the novel object recognition test and the Y-maze test. Additionally, oxidative stress marker levels, acetylcholinesterase (AChE) activity, choline acetyltransferase (ChAT) and glutamate receptor 1 (GluR1) expression were determined. Consequently, the findings demonstrated that the administration of pinostrobin (20 and 40 mg/kg) markedly improved cognitive function as indicated by an increase in recognition index and by spontaneous alternation behaviour. Pinostrobin also modulated the levels of oxidative stress by causing a decrease in malondialdehyde levels accompanied by increases in superoxide dismutase and glutathione activities. Similarly, pinostrobin markedly enhanced cholinergic function by decreasing AChE activity and promoting ChAT immunoreactivity in the hippocampus. Additionally, the reduction in GluR1 expression due to scopolamine was diminished by treatment with pinostrobin. The findings indicated that pinostrobin exhibited a significant restoration of scopolamine-induced memory impairment by regulating oxidative stress and cholinergic system function. Thus, pinostrobin could serve as a potential therapeutic agent for the management of neurodegenerative diseases such as Alzheimer's disease.