Aloe Vera Research Articles

Unveiling the Latest Breakthroughs: A Comprehensive Review of the Therapeutic Activity and Safety Profile of Aloe vera.

The use of herbal drugs as alternative and complementary medicine has increased in popularity, raising concerns about their safety profile. Aloe vera, a plant with diverse therapeutic properties, has been extensively used for centuries. This review aims to assess the therapeutic activity and safety profile of Aloe vera. A comprehensive literature search was conducted to gather relevant information from various biomedical databases. The chemical composition, mechanism of action, and therapeutic activities of Aloe vera were analyzed. Aloe vera contains numerous active components such as vitamins, enzymes, minerals, sugars, lignin, saponins, and anthraquinones. Its mechanisms of action involve collagen synthesis, anti-inflammatory effects, immune modulation, laxative properties, and antiviral activity. Aloe vera has demonstrated potential therapeutic benefits in wound healing, diabetes management, liver and kidney protection, and glycemic control. However, it is essential to consider potential side effects, such as skin irritation and allergic reactions. This review provides evidence-based information to improve patient safety and promote informed decisions regarding the use of Aloe vera as a therapeutic agent.

Herbal tooth gel formulations: A comprehensive review and comparative analysis for contemporary oral health practices

The shift towards natural healthcare has sparked renewed interest in herbal tooth gel formulations as alternatives to conventional oral care products. This comprehensive review explores the historical evolution, therapeutic properties, and contemporary significance of herbal tooth gels in oral health practices. Emphasizing the global trend towards natural remedies, the review delves into the efficacy of diverse herbal ingredients such as turmeric, camphor, and clove in managing oral health concerns, particularly periodontal diseases like gingivitis. Drawing from traditional practices and modern scientific research, the review highlights the advantages of herbal toothpaste formulations, including their gentle impact on teeth, absence of harmful chemicals, and diverse therapeutic properties. Moreover, the review discusses the sensory, physical, and antimicrobial properties of herbal tooth gels, examining their potential in plaque control and gingival health. By conducting a comparative analysis and evaluation of herbal elements such as neem, aloe vera, and triphala, the review highlights the increasing popularity and therapeutic possibilities of herbal toothpaste formulations in enhancing overall oral health. Amidst the prevalence of fast-paced lifestyles and common oral health issues, it's imperative to explore the extensive benefits provided by herbal tooth gels in contemporary oral healthcare. This becomes especially crucial given the rising global fascination with herbal remedies. Delving into the potential of herbal ingredients such as neem, aloe vera, triphala, guvava etc is particularly vital for addressing the challenges posed by poor oral health. Such exploration could hold promising solutions for enhancing overall oral hygiene and tackling oral health issues effectively.

Anti-gingivitis natural products: Aloe vera gel, tea tree oil mouthwash, turmeric gel, myrrh extract/mouthwash

Gingivitis is an inflammatory gum disease which occurs around the cervical portion of the gum and is classified by its clinical appearance. Gingivitis is very common, and anyone can be affected including but not limited to poor oral care habits. This paper reviews the potential of Aloe Vera, Tea Tree Oil Mouthwash, Turmeric Gel, and Myrrh Extract/Mouthwash. The study also assessed the knowledge and opinions of first-year pharmacy students. The survey part of the study included 5 knowledge-based and 5 opinion-based questions, focusing on the four herbal remedies. The knowledge-based questions showed a 65.7% correct response rate, with the question on turmeric receiving the highest accuracy (94.3%), while the question surveying the participants’ knowledge about the source of information for herbal products received the lowest correct answer rate at 31.4%. Opinion-based questions revealed a positive attitude towards herbal remedies, with about 67.6% to 94.3% strongly agreeing, or agreeing with the potential benefits of the products. The highest agreement rate at 94.3% was obtained for the statement that myrrh can help reduce inflammation in the gums and inhibit the growth of bacteria, thus assisting in the management of gingivitis. Disagreement of opinions was minimal, ranging from 5.7% to 32.3%, indicating a widespread inclination towards positive agreement.

The Potential of Aloe Vera in Solution and in Blended Nanofibers Containing Poly (3-Hydroxybutyrate-Co-3-Hydroxyvalerate) as Substrates for Neurite Outgrowth.

This pilot study investigated the potential of aloe vera (AV) to promote neurite outgrowth in organotypic dorsal root ganglia (DRG) explants (n = 230) from neonatal rats (n = 15). Using this invitro model of acute axotomy, we assessed neurite outgrowth exceeding 1.5 times the explant diameter (viable explants) and measured the longest neurite length. These parameters were evaluated under control conditions and in cultures supplemented with commercial AV and four aligned scaffolds: poly-L-lactate (PLLA), polydioxanone (PDS), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and blended PHBV/AV. After 6 days of culture, explants were immunostained using neuron-specific marker Tuj1 and Schwann cell marker S100. Measurements were obtained with Image J software and analyzed using Jamovi 2.3. In control and AV dilution media, the study revealed radial tissue growth from the explant body with randomly oriented neurites, whereas in all scaffolds, bidirectional tissue growth occurred parallel to nanofibers. Binomial logistic regression analyses indicated that viable explants were more likely in the control group compared to PDS (p = 0.0042) and PHBV (p < 0.0001), with non-significant differences when compared to AV dilution, PLLA, and PHBV/AV. AV dilution showed a greater association with viable explants than PLLA (p = 0.0459), while non-significant difference was found between AV dilution and PHBV/AV. Additionally, the PHBV/AV scaffold predicted higher odds of viable explants than PLLA (p = 0.0479), PDS (p = 0.0001), and PHBV (p < 0.0001). Groups with similar probabilities of obtaining viable explants (control, AV dilution, and PHBV/AV) exhibited non-significant differences in their longest neurite lengths. In conclusion, control, AV dilution, and PHBV/AV yielded the highest probability of developing viable explants and the longest neurite lengths. This is the first study demonstrating the direct permissiveness of AV for axonal outgrowth. Furthermore, the blended PHBV/AV scaffold showed significant potential as a suitable scaffold for axonal regrowth and Schwann cell migration, ensuring controlled tissue formation for tissue engineering applications.

Fabrication of a molecularly imprinted poly(methyl methacrylate) decorated graphite electrode for detection of aloe emodin in Aloe vera

In this work, the authors represent a comprehensive approach for the development and validation of a novel graphite-supported molecularly imprinted poly(methyl methacrylate) electrode for the selective and sensitive detection of aloe emodin (AE) in aloe-based cosmetic products. The electrocatalytic activity of the electrode was evaluated using cyclic voltammetry and differential pulse voltammetry techniques in phosphate buffer saline. Surface morphology, structural characteristics, and imprinting endorsement were investigated using field emission scanning electron microscopy, Fourier-transform infrared spectroscopy, and UV-Vis spectroscopy. Under the optimal conditions, the electrode showed an oxidative differential pulse voltammetry peak at -0.2931±0.011 V (vs. Ag/AgCl, saturated KCl). The current was increased linearly with increasing concentrations of AE from 0.0005 to 350 µM, with a low detection limit of 0.0003 µM. The voltammetric analysis has been validated by reverse-phase high-performance liquid chromatography (RP-HPLC) methods. A partial least square regression model was developed to correlate the results obtained from the proposed system with the reference values obtained by RP-HPLC. The model showed a high prediction accuracy of 95.95 % for the detection of AE in cosmetic samples. The developed electrode provides a low-cost, rapid, easy, and convenient method for the detection of AE in cosmetic products, offering potential benefits for quality control and safety assessment in the cosmetic industry.

Comparative Analysis of Corrosion Inhibition Efficiency Between Epoxy Paint and Organic Varnish Blended with Oils of Aloe vera and Elaeis guineensis Applied in Cooling Systems

Comparative Analysis of Corrosion Inhibition Efficiency Between Epoxy Paint and Organic Varnish Blended with Oils of Aloe vera and Elaeis guineensis Applied in Cooling Systems

Frequency and temperature-dependent dielectric behaviour of fresh Aloe vera at 1 to 20 GHz microwave frequency using time domain reflectometry

Frequency and temperature-dependent dielectric behaviour of fresh Aloe vera at 1 to 20 GHz microwave frequency using time domain reflectometry

Novel anti-tumor effect of natural products from Aloe vera resin and their in-vitro/in-silico targeting mechanism of carbonic anhydrase-II and IX.

Human carbonic anhydrase (hCA) plays a vital role in the development and progression of tumors in hypoxic conditions. Herein we report the hCA-II and hCA-IX activities of natural products isolated from Aloe vera (L.) Burm.f., to know their potential in tumors. These isolated compounds (1-10) displayed varying degrees of inhibition against hCA-II and hCA-IX. All the compounds showed potent activity against hCA-IX with IC50 values in the range of 2.9 - 29.1 µM. While for hCA-II, compounds 1, 2, 5-10 exhibited IC50 in the range of 4.7 - 23.4 µM. The most effective hCA IX and II inhibitors, 2 and 5, were chosen for in vitro mechanism studies, revealing that they are competitive inhibitors. Furthermore, when tested for their cytotoxic effect on BJ (normal) cell line, all the compounds showed no cytotoxic behavior, while on Prostate cancer cells (PC-3), compounds 1, 3, 5, 7, and 9 exhibited significant antiproliferative activity. Molecular docking was also conducted within the hCA IX and hCA-II active sites to observe their binding capability. Compounds 1, 5, 7, and 9 were active against both isozymes of hCA and in the PC-3 cell line, therefore these are the best choices for further in vivo studies..

Fabrication, Characterization and Release Profile of Aloe Vera Extracts/PVA Composite Electrospun Nanofiber

Aloe vera is a well-known remedy that carries many beneficial health effects such as painkiller, anti-inflammatory, promote skin growth and repair. The combination of bioactive natural product of aloe vera as a drug model and polyvinyl alcohol (PVA) as the base material or carrier in the electrospinning process were studied. Smooth straight and continues electrospun fibers were collected with the Field Effect Scanning Electron Microscope (FESEM) images show no formation of bead (defect signed) in the electrospun membrane when the concentration was set at 10% w/w of PVA nanofibre. The morphological structure of the electrospun membranes shows a smooth and longitudinal fiber when aloe vera is mixed with the PVA polymer with percentage ratio of aloe vera over PVA is less than 25%. The Fourier Transform Infrared Spectroscopy (FTIR) shows no reaction between PVA and aloe vera by not showing peaks other than the initial materials. The Differential Scanning Calorimetry (DSC) proves the presence of aloin indicating the presence of aloe vera in nanofiber. The release profile of the electrospun aloe vera in PVA shows a higher initial burst release at the 50% and 70% concentration levels indicating very little control of the release or none at all. These results show the potential of aloe vera – PVA electrospun nanofibers membrane as a promising material for wound dressing and topical drug delivery.

Effectiveness of the Application of Lanolin, Aloe Vera, and Peppermint on Nipple Pain and Nipple Trauma in Lactating Mothers: A Systematic Review and Meta-Analysis.

Infants who are not exclusively breastfed are more vulnerable to gastroenteritis, respiratory illness, and type 1 diabetes mellitus. Mothers who do not breastfeed their infants are at a higher risk of cancer. This systematic review and meta-analysis aimed to synthesize evidence regarding the effectiveness of applying lanolin, aloe vera, and peppermint for alleviating nipple pain and nipple trauma among lactating mothers. Electronic databases, including PubMed (MEDLINE), Cochrane, Cumulative Index to Nursing and Allied Health Literature (CINAHL), EMBASE, Web of Science, Scopus, and Clinical Key, were searched for studies published in English from January 2000 to December 2022. Nipple pain and nipple trauma were the primary outcomes analysed, and a meta-analysis was conducted via RevMan v5.4. The reviewed trials involved 1,495 participants from 11 studies, with an average participant age of 29 ± 5 years. The application of lanolin significantly reduced nipple pain and nipple trauma in lactating mothers. The application of peppermint significantly reduced nipple trauma in lactating mothers. The application of lanolin or peppermint is a beneficial preventive intervention for reducing nipple trauma and pain.

Enhancing Wound Healing with Nanohydrogel-Entrapped Plant Extracts and Nanosilver: An In Vitro Investigation

Wound healing is a complex process that can be improved through advanced biomedical approaches. Incorporating nanopolymers and plant extracts into wound dressings offers a favorable strategy for promoting tissue repair. Nanopolymers provide a controlled environment for sustained drug release while also protecting the wound from external contaminants. When combined with bioactive compounds from plant extracts, which possess antioxidant, anti-inflammatory, and antimicrobial properties, this hybrid approach can accelerate healing, reduce infection, and improve tissue regeneration. Hence, in this study, we have synthesized alginate/gelatin hydrogel blended with only nanosilver (Alg/gel-Ag) and with nanosilver and plant extracts like aloe vera, curcumin, plantain peel extract, and Calendula flower petal extract (Alg/gel-AgP). The synthesized hydrogels were characterized using different photophysical tools, and the cytotoxicity effect was studied using a fibroblast cell line (V79). The antibacterial effect of the hydrogels was also observed against E. coli and S. aureus, determining the MIC and MBC. The wound healing in vitro was also assessed using scratch assay which depicted a rapid wound closure for Alg/gel-AgP compared to the untreated control and Alg/gel-Ag. The combined effect between nanotechnology and natural extracts represents a novel and effective approach for enhancing the wound healing process.

Gastroprotective Efficacy of North African Medicinal Plants: A Review on Their Therapeutic Potential for Peptic Ulcers

ABSTRACTPeptic ulcer disease remains a prevalent gastrointestinal disorder worldwide. Current treatments often have limitations, sparking interest in alternative therapies from medicinal plants. This review examines the gastroprotective potential of 54 North African medicinal plants against peptic ulcers. An extensive literature search was conducted, focusing on plants with preclinical and clinical evidence of anti‐ulcer efficacy and documented use in North African traditional medicine. The review identified several promising plant species, such as licorice (Glycyrrhiza glabra), chamomile (Matricaria chamomilla), olive (Olea europaea), pomegranate (Punica granatum), Aloe vera, and black seed (Nigella sativa), along with their bioactive constituents, including flavonoids, tannins, and terpenoids. These compounds exhibit gastroprotective properties through multiple mechanisms, such as enhancing the gastric mucosal barrier, inhibiting acid secretion, displaying antioxidant and anti‐inflammatory effects, promoting ulcer healing, and combating Helicobacter pylori infection. The evidence presented includes in vitro assays, animal models, and some clinical studies. While many of the 53 plants reviewed demonstrated significant anti‐ulcer effects compared to standard drugs, further clinical research is needed to establish efficacy and safety in humans. The synergistic actions of phytochemical mixtures in medicinal plant extracts likely contribute to their therapeutic potential. This review highlights the role these North African medicinal plants may play in the prevention and treatment of peptic ulcers and identifies promising candidates for further research and development of evidence‐based botanical therapies.

Evaluation of the Wound Healing Potential of Aloe Vera Leaf Extract Containing Carbopol 934 as a Gelling Agent and Comparing with Sodium CMC and HPMC on Surgically Induced in Wound Wister Rats: A Preclinical Study

ABSTRACT Introduction: The restricted injury can occur in any tissue or organ and is caused by an external source. The toughness and adaptability of the delivery system are enhanced by the synthetic hydrophilic, biocompatible, and biodegradable stable polymer known as carbopol 934. Aloe vera is a plant belonging to the Liliaceae family that releases anthraquinones, a bitter liquid that has medicinal qualities. Aim: The current study is to determine if aloe vera gel containing Carbopol 934 can cure wounds in animal models used in experiments. Need of the Study: This review will offer a thorough examination of recent studies on the medicinal use of Aloe vera gel. Further study is required to establish the mechanism of action because certain areas, such as wound healing, have not received much attention in the scientific community. However, these biological materials must first be appropriately isolated and identified. Materials and Methods: Various centralizations of Carbopol 934, sodium CMC, and HPMC will be attempted, and lastly, gel that showed great spreadability and consistency will be chosen for wound mending properties of homegrown Aloe vera gel.

Second‐degree burns treatment: Preclinical in vivo approaches using natural latex rubber containing Aloe vera extract

AbstractNatural rubber latex (NRL) obtained from Hevea brasiliensis is emerging as a viable and economical alternative for biomedical treatments, particularly for dermal injuries. Its effectiveness is enhanced when combined with bioactive molecules, such as those found in Aloe vera (AV), known for their healing properties and are commonly used in low‐cost wound healing therapies. Thus, this study evaluated the physicochemical and biological properties of NRL incorporated with 25%, 35%, and 50% AV, both in vitro and in vivo. FTIR analyses revealed only physical interactions between NRL and AV, facilitating their release, as evidenced by the release profile. In vitro assays indicated improved biocompatibility in samples composed of 25% and 35% AV, while complete cell death occurred at 50% AV. Histological evaluations of second‐degree burn models in rats showed greater re‐epithelialization of the epidermis with 35% AV after 14 days of treatment. However, 50% AV highlighted the importance of avoiding overdosing to prevent tissue necrosis. Despite the differences observed between in vitro and in vivo results, NRL membranes containing AV are a promising approach for future clinical trials in skin wounds treatment, offering an effective and affordable solutions for skin regeneration in clinical settings.

A Review on Formulation of Body Lotion

The body is shielded by protective layers of skin. A plant-based herbal body lotion provides soothing hydration. Common ingredients often feature aloe vera, known for its healing properties, pain relief, and moisture retention. For centuries, it has been used to treat skin burns and wounds. Aloe vera, honey, glycerin, rose water, and triethanolamine were selected for the formulation of the herbal body lotion. Evaluation parameters were conducted to ensure that the formulation is safe for human use. The aloe vera body lotion was created using these ingredients, which include aloe vera known for its antimicrobial and hydrating properties, protecting the skin from microbial damage while providing moisture. In conclusion, this herbal body lotion is designed for topical application. Aloe vera enhances the lotion's synergistic and moisturizing effects on the skin. Herbal remedies are gaining worldwide popularity, and the combination of aloe vera, honey, coconut oil, Lavender oil, and glycerin in this formulation is an excellent approach. These formulations were assessed using various evaluation parameters, including homogeneity, appearance, after-feel, acid value, pH measurement, irritancy test, viscosity, accelerated stability testing, subjective properties, spreadability, type of emulsion test, sensitivity test, washability test, statistical analysis, in vitro permeation studies, thermal stability tests, total fatty matter determination, water content analysis, and patch tests. The aim of this review is to compile information on different herbal lotion formulations and their evaluations. Numerous researchers have studied herbal lotion formulations, and this knowledge can assist other researchers in developing novel herbal cosmetic formulations featuring new ingredients.

Analysis of biological activities of Aloe vera gel and extract used as the potential use in natural food additives

El propósito de este estudio es evaluar las propiedades antimicrobianas del gel de Aloe vera (AVG) y el extracto de Aloe vera (AVE). En el contexto de la seguridad alimentaria, el enfoque principal ha sido el uso potencial de estos productos naturales como conservantes de alimentos y sus efectos a nivel microbiano. Como parte del estudio, se prepararon AVG y AVE en diferentes concentraciones (1, 2, 3, 4 y 5% p/v). Los microorganismos utilizados en las pruebas incluyeron Salmonella spp., Staphylococcus aureus, Escherichia coli, Bacillus subtilis subsp. spizizenii, Candida albicans y Aspergillus niger. Los análisis microbiológicos se realizaron de acuerdo con las normas ISO y las cargas microbianas se evaluaron en diferentes diluciones. El análisis estadístico de los datos se realizó mediante la prueba de rangos con signos de Wilcoxon, la prueba no paramétrica de Friedman y el ANOVA bidireccional. Se descubrió que ambas formas de AVG y AVE eran efectivas contra ciertas bacterias y hongos probados. Específicamente, la forma de gel de AVG mostró efectividad contra B. subtilis y E. coli, mientras que la forma de extracto fue ineficaz contra estos microorganismos. Los análisis estadísticos indicaron que el tiempo es un factor importante en la eficacia antimicrobiana de AVG y AVE. El estudio presentó hallazgos que respaldan el uso potencial de AVG y AVE como conservantes de alimentos.

Analysing the feasibility of agro-based value chain configurations in the rural context with B2B marketing

Purpose This paper aims to advocate for small-scale entrepreneurship with commercial cultivation to earn better profits at the farmer level. This paper explores potential value chain configurations of vertical coordination. Design/methodology/approach The aloe vera supply chain was examined for the study where aloe vera juice is the product. The various configurations are complete vertical coordination, semi-vertical coordination and non-vertical coordination. These were analysed to find feasibility and market prospects based on parameters. The cyclic view of supply chains, comparative analysis and fuzzy-analytical hierarchy process algorithm are presented. Findings The semi-vertically coordinated supply chain is indicated as the optimum setup to initiate value chains. The entrepreneurs in this configuration will be able to sell semi-finished produce to a brand that will process and distribute the final product. This B2B setup will help the farmer to earn better revenues in the post-COVID-19 scenario. Effective marketing with improved execution and innovation will boost the food processing industry. Research limitations/implications This study uses an established methodology to distinguish among alternatives based on underlying factors. The study is based on data collected from stakeholders. The study reflects the most accurate picture of real-world scenarios at the farm and market level in the Indian context. Practical implications This study’s learnings will help understand the organisational approach to serve demand by designing unique business configurations. Adapting the fundamental model with technological intervention will improve supply chain performance and sustainability. The most modern approaches in agri-business, such as subscription models, vertical farming, corporate entrepreneurship and technological intervention, will be covered in future work. Social implications This study will help stakeholders, including the government, decide whether to introduce training skills, subsidy policies, business expansion ideas and feasibility studies. Originality/value The study highlights the strategic role of developing the B2B platform to support customer values and customer ecosystem with better product availability.

The Effect of Aloe vera on Skin and Its Commensals: Contribution of Acemannan in Curing Acne Caused by Propionibacterium acnes

Aloe vera is one of the most significant therapeutical plant species that belongs to the family Liliaceae. Aloe vera is composed of a high amount of water, with the remainder being dry matter. The dry matter contains a lot of bioactive compounds like carbohydrates, fats, and enzymes, with various therapeutic and antimicrobial properties. It can enhance the proliferation of cells and prevent cell damage by anti-oxidative properties (stimulating the secretion of superoxide dismutase and peroxidase). Human skin is colonized by microbes like fungi (Candida albicans), bacteria (Propionibacterium acnes, Staphylococcus aureus), and mites. These commensals are responsible for skin characteristics such as acidic pH, the pungent smell of sweat, etc. Human fetuses lack skin microbiota, and their skin is colonized after birth. Commensals present on the skin have a crucial role in training the human immune system against other pathogenic microbes. Propionibacterium acnes act as an opportunistic pathogen when the balance between the commensals is disturbed. We also emphasize the recent progress in identifying the aloe metabolite biosynthesis pathways and the associated enzyme machinery. The hyperproliferation of Propionibacterium acnes causes acne, and acemannan plays a significant role in its cure. Hence, we need to consider a new treatment approach based on the root cause of this dysbiosis.

Plant-Based Flocculants as Sustainable Conditioners for Enhanced Sewage Sludge Dewatering

With the aim to establish clean and sustainable sludge treatment, green conditioning using natural flocculants has recently gained a growing interest. In this study, a variety of plant materials, namely Moringa (Moringa oleifera) seeds, Fenugreek (Trigonella foenum-graecum) seeds, Potato (Solanum tuberosum) peels, Aloe (Aloe vera) leaves, Cactus (Opuntia ficus indica) cladodes, and Phragmites (Phragmites australis) stems, were evaluated for their potential bioflocculant activity in conditioning sewage sludge. They were thoroughly characterized to determine their active flocculating compounds. Sludge dewaterability was evaluated by assessing various sludge parameters, including specific resistance to filtration (SRF), dryness of filtration cake (DC), and total suspended solid removal (TSS) from sludge filtrate. The collected results from various physicochemical characterizations of plant materials suggest that the main flocculating agents are carbohydrates in Cactus and Fenugreek and proteins in Moringa, Potato, and Phragmites. Additionally, all tested plant-based flocculants demonstrated effective dewatering performance. Interestingly, compared to the chemical flocculant polyaluminum chloride, Moringa and Cactus showed superior conditioning effects, yielding the lowest SRF values and the highest DC. As a result, the use of these natural flocculants improved sewage sludge filterability, leading to a significant removal of total suspended solids from the filtrate. The conditioning properties of Moringa and Cactus can be attributed to their high protein and sugar content, which facilitates the effective separation of bound water from solids through charge neutralization and bridging mechanisms. Thus, green conditioning using plant-based flocculants, particularly Moringa and Cactus materials, presents a promising and eco-friendly approach to enhance sewage sludge dewatering for safer disposal and valorization.

The Influence of Aloe Vera Biocoagulant and Mixing Time in Reducing Biological Oxygen Demand, Chemical Oxygen Demand and Total Suspended Solids Levels

Aloe vera is a plant with complex composition of carbohydrates, sugars, and mucilage, enabling it to bind particles in water and serve as a coagulant in the coagulation-flocculation process. This study aims to assess the impact of varying doses and rapid stirring times on the reduction of biochemical oxygen demand (BOD), chemical oxygen demand (COD), and total suspended solids (TSS) levels in liquid waste from pharmaceutical companies. The research involves two factors: (1) aloe vera concentration at three levels (20 mL/L, 40 mL/L, and 60 mL/L); (2) fast mixing time at three levels (20 minutes, 40 minutes, and 60 minutes), with each treatment repeated thrice. Levels of BOD, COD and TSS were measured post-treatment using a jar test tool. The results of the analysis showed that the optimal efficacy of the aloe vera coagulant occurred at a concentration of 40 mL/L for 40 minutes. At this optimal condition, the effectiveness of the aloe vera coagulant in reducing BOD, COD and TSS levels was 72.3%, 78.5%, and 65.3%, respectively. This indicates that the aloe vera coagulant can be effectively utilized in the treatment of pharmaceutical industrial wastewater to reduce BOD, COD and TSS levels