Extract Of Aloe Barbadensis Research Articles

Antilipidemic Effect of Ethanolic Extract of Aloe barbadensis Gel, Cymbopogon citratus Leaves and Its Combination on Alloxan Induced Diabetic Rats

This research sought to investigate the antilipidemic effect of ethanolic extract of Aloe barbadensis, Cymbopogon citratus leaves and its equal combination on induced-alloxan diabetic rat. Plants like Aloe barbadensis and Cymbopogon citratus are commonly used as home remedies for some diseases such as diabetes thereby making them alternative medicines. Ethanolic extract of both plants was extracted using 70% ethanol. The effect of glibenclamide, ethanolic extract of A. barbadensis gel, C. citratus leaves and its combination after 21 days experimental study showed a significantly increased (P<0.05) high density lipoprotein (HDL) in the induced rats when in comparison with the normal control and diabetic untreated rats. The low density lipoprotein (LDL) significantly showed a (P<0.05) decrease in the rats treated with A. barbadensis gel, C. citratus leaves and its combined extract compared with those treated with standard drug. The serum total cholesterol (TCHOL), triglyceride (TG) and very low density lipoprotein (VLDL) significantly showed a (P<0.05) decrease in the rats treated with A. barbadensis gel, C. citratus leaves and its combined extract compared with diabetic untreated rats. This research revealed that ethanolic extract of A. barbadensis, C. citratus and its combination has antilipidemic effects and holds potential as an alternative therapy for its management.

Revolution of Bone and Teeth Health: Study of Aloe Barbadensis Instant Powder Formulation

Aloe barbadensis is a plant with many applications such as anti-inflammatory, anti-fungal, anti-bacterial, and aiding in cell regeneration. Understanding aloe barbadensis' phytochemical profile and pharmacological action is essential since it is believed to have an impact on the formation of teeth and bones. The stability and bioavailability of Aloe barbadensis can be improved by formulating it as an instant powder. The research's objectives are to ascertain the Aloe barbadensis instant powder's qualitative and quantitative phytochemical profile, dosage formulations, and activity testing on hemoglobin, cholesterol, and red blood cell parameters. Samples of aloe barbadensis were washed, and they were then dried for 72 hours at 50°C. Following a maceration process using a 70% ethanol solvent, the extract was dried. Phytochemical screening, TLC profile, and extract description were employed to test the extract qualitatively. The quantities of total flavonoids, total anthraquinones, and total phenolics were determined to quantitatively test the extract. The formulation of the instant powder was then completed and evaluated on female mice using metrics associated with red blood cells, hemoglobin, and cholesterol levels. Furthermore, observations were made on the mice's liver organs. The study's findings revealed a qualitative profile of Aloe barbadensis extract, which included a tasteless, unique odor, milky white hue, and liquid shape. Aloe barbadensis has been demonstrated to contain flavonoids, phenolics, tannins, saponins, and anthraquinones, according to the results of phytochemical screening. Three spots, identified as Rf 3.2 and Rf 8.5 in the Rf 2.3 area, are visible in the chromatographic pattern. Total anthraquinones were discovered to be 4.59%, total flavonoids to be 0.24%, and total phenolic content to be 1.42%. The third formula of instant Aloe barbadensis pollen has been demonstrated through preclinical examinations to have the capacity to reduce cholesterol, boost hemoglobin, and enhance red blood cell count—all of which are associated with the growth of teeth and bones. An SPSS statistical study demonstrating statistically significant differences with other groups supports this. Mice liver histopathological examinations revealed no liver damage in any of the test groups.

Effects of aqueous leaves extract of Aloe barbadensis on blood glucose levels of streptozocin-induced diabetic Wistar rats

The study was to investigate the antidiabetic of aqueous extract of leaves of plant Aloe barbadensis. In STZ induced diabetes in rats, a study was conducted on six groups of six male Wister rats each to evaluate the hypoglycaemic effect. Diabetes was induced in rodents by intravenous (i.v) infusion of STZ at a portion of 50 mg/kg body weight, disintegrated in 00.10M virus citrate cushion (pH = 04.50). Blood glucose level was estimated utilizing GOD-POD (Kit spin react). Blood tests were pulled back from the retro-orbital plexus under light ether. A. barbadensis (ALEC) was exposed to hostile to diabetic action in rodents where STZ was utilized as a portion of 120 mg/kg in intraperitoneal portion as a diabetogenic specialist to an acquainted diabetic with test rodents. In intense streptozotocin prompted models fasting blood glucose level was recorded on multi-day as basal worth 4th, 7th and 10th day. Aqueous extracts of leaves of A. barbadensis at portion 250 and 500 mg/kg showed portion subordinate critical enemy of hyperglycemic action on 4th, 7th and 10th day post-treatment. ALEC portion of 100 mg/kg likewise caused a decrease in blood glucose level; however, outcomes were found factually non-noteworthy. The Antihyperglycemic impact of fluid leaves concentrate of A. barbadensis at portion 100 mg/kg was discovered less viable than reference standard medications glibenclamide. The most extreme decrease was seen on the 10th day of the greatest portion of 500 mg/kg ALEC. Glibenclamide produces a critical decrease in blood glucose level in contrast with diabetic control. Glibenclamide was used as a standard drug, and the results were compared in reference to it. The study confirmed the aqueous extract of Aloe barbadensis has significant and sustained oral hypoglycaemic activity, comparable with the hypoglycaemic effect of glibenclamide, a sulfonylurea derivative.

Agro-Industrial Waste as a Source of Raw Material: Eggshell and Ash of Useful for the Synthesis of Hydroxyapatite

Agave salmiana ash and poultry eggshell powder as CaO sources were used for obtaining nanostructured hydroxyapatite (HAP). The synthesis was carried out by the Green Chemistry Hydrothermal Biosynthesis at 180°C with a pH of 5, by reacting CaO from Agave Salmiana ash and Eggshell powder, with dibasic calcium phosphate (CaHPO4•2H2O) in an aqueous solution, with Aloe barbadensis extract. The product was characterized by X-ray diffraction, Fourier-transform infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). The size and shape of the hydroxyapatite particles changed dramatically in the presence of Aloe barbadensis. Large crystals of Hydroxyapatite were observed when Eggshell powder and Agave salmiana ash were used as raw materials in the presence of the Aloe barbadensis surfactant. Crystals with shapes of ribbons and plates from 1 micrometers to 8 micrometers were observed when using the eggshell powder in the presence of Aloe barbadensis and, in the case of Agave salmiana ash in the presence of Aloe barbadensis, crystals with shapes of quadrangular prisms and hexagonal (polyhedra) with sizes from 2 micrometers to 20 micrometers were observed. Hydroxyapatite was therefore successfully biosynthesized by a green and sustainable method that reduces the environmental impact.

Study on the In vitro and In vivo Antifungal Activities of Nano-silver against Mycoleptodiscus indicus causing Leaf Blight on Lotus in Vietnam

Background: Lotus, Nelumbo nucifera Gaertn is Vietnam’s symbol and Buddhism’s flower and plays an essential part in rural Vietnam’s economy as all aspects of lotus could bring benefits to farmers. But, unfortunately, lotus yield in Vietnam is seriously affected by various plant diseases. Among them, leaf blight is currently emerging as one of the primary diseases devastating lotus crops in Vietnam, in which there are large necrotic parts on lotus leaves, flowers and seeds. Methods: Aloe barbadensis extract was used for synthesizing silver nanoparticles. Leaf blight lotus leaves were collected to isolate pathogenic fungi-infection of isolated pathogenic fungi on the healthy lotus and then identify hidden mold by 28S rRNA sequencing. Determination of in vitro minimal inhibition concentration of nano-silver was conducted according to Azizi. The reduction of disease symptoms and biological characteristics of the treated lotus was observed. Result: Morphological analysis and molecular identification of 28S rRNA sequencing showed that the pathogenic microorganism was Mycoleptodiscus indicus (M. indicus). Both in vitro antifungal activity and in vivo treatment of leaf blight lotus using a nano-silver solution showed that 30 ppm of nano-silver was the minimal inhibition concentration (MIC) for totally eradicating M. indicus growth. This was the first time M. indicus was reported to infect and cause leaf blight on a lotus. Previously, M. indicus was a well-known plant pathogen that could cross-kingdom infect humans and animals. Thus, the fact that lotus is widely cultured in Vietnamese rural could increase the chance for M. indicus to spread; hence, this raised the alarm about its potential harm to plants, humans and animals. And, significantly, it revealed nano-silver as a possible approach to prevent M. indicus.

Gastro-protective actions of Aloe barbadensis Miller mitigate ethanol-induced gastric injury in rats

Purpose: To investigate the gastroprotective effect of leaf extract of Aloe barbadensis on ethanolinduced gastric ulcer in rats.Methods: Healthy male Wistar rats (n = 30) weighing 180 - 220 g (mean weight = 200 ± 20 g) were randomly assigned to 6 groups (5 rats/group): control group, gastric ulcer group, two Aloe barbadensis extract groups (250 and 500 mg/kg), cimetidine group and indomethacin group. Gastric ulcer was induced via oral injection of absolute ethanol at a dose of 1 mL/kg after a 24-h fast. Gross evaluation, determination of gastric juice acidity and histological examination of gastric tissue were carried out.Results: Treatment of gastric ulcer rats with Aloe barbadensis extract significantly reduced ulcerated area (UA), ulcer index (UI), and acidity of gastric juice (p < 0.05). Injection of 1 % carrageenan into rat hind paw led to a time-dependent increase in paw volume which peaked 3 h after injection. However, the Aloe barbadensis extract significantly and dose-dependently reduced the volume of inflamed paw, and inhibited edema formation (p < 0.05). It also markedly reduced or completely eliminated edema and leucocyte infiltration. Moreover, treatment of gastric ulcer rats with Aloe barbadensis leaf extract led to significant and dose-dependent reduction in gastric tissue MDA level (p < 0.05). Histological examination of the gastric wall showed that control rats had severe injury in gastric mucosa,accompanied by edema and leucocytes infiltration, relative to rats pretreated with extract which showed marked gastric protection and inhibition of edema and leucocytes infiltration. Moreover, the extract treatment protected the gastric surface against ulceration as indicated by reduced lesions in the treated rat model.Conclusion: These results show that Aloe barbadensis mitigates ethanol-induced gastric injury in rats via inhibition of lipid peroxidation and inflammation. Thus, the extract has potentials for development into a therapeutic agent for the management of gastric ulcer.
 Keywords: Aloe barbadensis, Gastric ulcer, Gastric mucosa, Inflammation, Lipid peroxidation

Geeta Bhandari and Shruti Baurai

Abstract-Exposure to sunlight can trigger various biological responses ranging from sun-burn, erythema to skin cancer. Synthetic sunscreen formulations available in market pose variety of adverse effects. Therefore,formulationofthe herbalsunscreenformulation and evaluation of its sun protection activity is an important aspect in the cosmetic industry. The purpose of present study was to evaluate the sun protection factor (SPF) of aqueous and methanolic extract ofAloe barbadensis miller andCocos nuciferaby ultraviolet (UV) spectroscopy method.Methanolic extract of Cocos nucifera showed the highest SPF value (3.2)amongst all extracts.The results indicated presence of active components responsible for ultraviolet absorption which may be extracted from these plant extractsand maybe used in sunscreens preparations for better protection against sun rays.Key Words: UVradiations, SPF, Aloe barbadensis miller andCocos nucifera

Pharmacological screening of anti-diabetic and antihyperlipidemic activity of aqueous extracts of leaves of Aloe barbadensis

The present study was to estimate the anti-diabetic and antihyperlipidemic activity of aqueous extract of leaves of plant Aloe barbadensis.In alloxan-induced diabetes in rats, the research examined six groups of six male wister rats every to value the hypoglycaemic effect of the barbadensis (ALEC) exposed to hostile to diabetic action in rodents where alloxan monohydrates were utilized as a portion of 120.00 mg/kg in intraperitoneal portion as a diabetogenic specialist to an acquainted diabetic with test rodents. In sub intense, treatment, bunch blood glucose levels are seen on 14.00th, 21.00th and 30.00th-day present treatment compare on typical control in diabetic control checked expanded blood glucose level was watched, with dosages of 100.00, 250.00 and 500.00 mg/kg b.w. A portion of 500.00 mg/kg b.w/day was seen as having the most significant activity. Glibenclamide was applied as a standard drug, and the outcome was compared about it. In the antihyperlipidemic activity, serum cholesterol levels were recorded at aqueous extracts of 100.00, 250.00 and 500.00 mg/kg, on 30.00th-day post-treatment. Serum cholesterol levels were ordinary benchmark groups was 107.70±01.21 mg/dl diabetic control 178.50±01.04 mg/dl and standard medications treatment bunches was 111.30±02.40 mg/dl and ALEC treatment bunches were 171.20±01.03 mg/dl, 145.80±02.92 mg/dl and 118.80±03.86 mg/dl for 100.00, 250.00 and 500.00 mg/kg separately. The results show that the aqueous extract of Aloe barbadensis has significant and continuous oral hypoglycaemic activity, equivalent to the hypoglycaemic result of glibenclamide, a sulfonylurea derivative and hyperlipidemic activity. Extracts also confirmed antihyperlipidaemic possible of the plant extract is establish to be comparable with that of the standard.

Neuroprotective effects of aqueous extract of <i>Aloe barbadensis</i> on the cortical cells of adult Wistar rats following monosodium glutamate-induced neurotoxicity

The study assessed the oxidative state and cellular changes of the cerebral cortex following monosodium glutamate-induced neurotoxicity in Wistar rats, with a view to evaluate the effects of varying doses of aqueous extract of Aloe barbadensis (AB) over a four-week period. Eighty Wistar rats (8 weeks old) were randomly assigned into 4 groups of 20 rats; Group 1 (Control group) received 3 mL/kg of distilled water. Groups 2, 3 and 4 received 3 g/kg/day of MSG dissolved in distilled water. In addition, Groups 3 and 4 received 100 and 200 mg/kg/day of AB extract respectively. Oral administration of the above lasted for 28 days in all groups. Five rats per group were sacrificed weekly for 4 weeks. The brain was harvested; one cerebral hemisphere was homogenised for oxidative state assessment. The other hemisphere was fixed in 10% neutral buffered formalin and stained with H&E and Cresyl violet. Data were analyzed using student t-test and p-value < 0.05 was considered significant. Malodialdehyde concentration and densities of degenerating neuron and oligodendrocyte were significantly higher in group 2 compared with group 1 over the 4-week period. No significant difference was noted between groups 1, 3 and 4 for same parameters. Nissl bodies depletion was noted in group 2 across the 4 weeks, lesser degree of depletion was noted in groups 3 and 4. This study concludes that oral administration of MSG resulted in increased lipid peroxidation and depletion of Nissl bodies resulting in degeneration of cortical cells. Aloe barbadensis at 100 mg/kg was sufficient to protect the brain.

Polyacrylamide-Metilcellulose Hydrogels Containing Aloe barbadensis Extract as Dressing for Treatment of Chronic Cutaneous Skin Lesions.

Chronic wounds are severe breaks in the skin barrier that fail to heal in an acceptable time-frame, thus preventing the complete restoration of the tissue’s anatomical and functional integrity, increasing the likelihood of infections and apoptosis. Hydrogels are known as a drug delivery system and have the potential to cover wounds and burns on the skin. Aloe barbadensis contains over 75 different bioactive compounds which are responsible for its anti-inflammatory and antimicrobial properties. In this study, the polyacrylamide-co-methylcellulose hydrogel containing Aloe barbadensis were developed. The extract was prepared from lyophilized Aloe barbadensis, using methanolic extraction, characterized by high performance liquid chromatography and incorporated into the hydrogels. These Aloe barbadensis hydrogels were characterized by degree of swelling, Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermal profiling using thermogravimetric analysis. The minimum inhibitory concentration test was done on the Aloe barbadensis extract to evaluate its antibacterial and antifungal activity in vitro. The Aloe barbadensis hydrogels and were shown to swell to almost 2000% of their original sizes. The Fourier-transform infrared spectroscopy indicated the presence of bands characteristic of Aloe barbadensis and hydrogel polymers. The basic hydrogel showed greater thermal stability than the hydrogels with Aloe barbadensis. The minimum inhibitory concentration showed inhibition of the growth of S. aureus and Salmonella spp. at specific concentrations. The hydrogel therefore presents itself as an excellent potential curative cover of cutaneous lesions.

Bio-Synthesis and Characterisation of Cuo Nanoparticles using Aloe Barbadensis

In the present work, green synthesis of copper oxide nanoparticles has wide interest due to its inherent features such as eco-friendly and low costs. Here we propose a cost effective and eco-friendly green synthesis of copper oxide nanoparticles using aloe barbadensis and copper acetate. Aloe barbadensis has antioxidant property that helps in the formation of nanoparticles. Aloe barbadensis extract was obtained by heating it in distilled water and mixed to copper acetate to form copper oxide nanoparticles. The synthesised nanoparticles were analysed using UV-Visible spectroscopy, X-ray diffraction studies (XRD) and Fourier-transform infrared spectroscopy (FTIR).

Synthesis of titanium oxide nanoparticles using Aloe barbadensis mill and evaluation of its antibiofilm potential against Pseudomonas aeruginosa PAO1

Titanium dioxide nanoparticles (TiO2-NPs) were synthesized using the aqueous leaf extract of Aloe barbadensis as a reducing and fabricating agent. The biosynthesis of the TiO2-NPs was initially confirmed by UV–vis spectroscopy. Based on the HRTEM and FESEM analysis, the biosynthesized NPs were found to be polydispersed and predominantly spherical in shape, with an average size of ~20 nm. A sharp and strong characteristic peaks of titanium (Ti) and oxygen (O) observed in the EDS pattern confirmed the synthesis of the TiO2-NPs. The FTIR spectroscopy suggested the presence of terpenoids, flavonoids and proteins which might be responsible for the biosynthesis and fabrication of the TiO2-NPs. The crystalline nature of the synthesized TiO2-NPs constituting of a mixture of brookite, anatase, and rutile phases was indicated by the XRD pattern. The spectral window around 180–1000 cm−1 covered the high-frequency Raman spectra of the TiO2-NPs. The Raman vibrational spectrum showed four Eg modes centered at 197.84, 399.24, 514.50, and 641.58 cm−1 representing the anatase phase of TiO2-NPs. The strongest and broadened peak of anatase was observed at the frequency of 641.58 cm−1. The metabolic activity of P. aeruginosa exposed to the MIC of TiO2-NPs was measured based on the reduction of tetrazolium salt by the dehydrogenase enzyme, produced by the metabolically active bacterial cells. The reduction in TTC was evident from the appearance of a red colored formazan in the solution. A noticeable suppression in the cell viability by 30.76 ± 3.96% of P. aeruginosa in the biofilm mode was found in presence of TiO2-NPs. Furthermore, the Minimum Inhibitory Concentration (MIC) of TiO2-NPs exhibited profound antibiofilm activity against P. aeruginosa by effectively preventing the adherence of the planktonic cells to the substratum. Thus, these NPs may be employed in controlling bacterial infections associated with biofilm.

Green Synthesis of Nickel–Copper Mixed Ferrite Nanoparticles: Structural, Optical, Magnetic, Electrochemical and Antibacterial Studies

The nickel–copper mixed ferrite nanoparticles [Cu1−xNixFe2O4 (x = 0, 0.5, 1)] NPs were prepared a by combustion method using Aloe barbadensis extract as a green reducing agent. The structural, functional, morphological, optical, magnetic, electrochemical properties of the sample were investigated using x-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, energy-dispersive x-ray spectroscopy, ultraviolet–visible (UV–Vis) spectrophotometry, photoluminescence, vibrating sample and cyclic voltammetry. The XRD patterns showed that all compositions with a cubic spinel structure and a crystallite size of 52 nm were reduced to 29 nm after nickel addition in copper ferrite and 35.85 nm for nickel ferrite. The UV–Vis absorption spectrum shows that the nickel-substituted copper ferrite band energy (Eg) increases as the crystallite size decreases. Measurements of magnetization obtained at room temperature revealed a soft ferromagnetic behaviour and saturation magnetization, coercivity value increased with the substitution of nickel. The maximum specific capacitance of 114 F g−1 was obtained at the scan rate of 5 mV s−1 for copper–nickel mixed ferrite NPs. A study of antibacterial activity against Escherichia coli, Klebsilla pneumonia, Staphylococcus aureus, and Bacillus subtilis using a well-diffusion method was performed. Nickel substitution on the copper spinel ferrite NPs revealed a major influence on structural, optical, magnetic, electrochemical and magnetic properties of the product obtained.

Adsorption of Congo Red (Acid Red 28) Azodye on Biosynthesized Copper Oxide Nanoparticles

A green synthesis of copper oxide nanoparticles and its removal efficiency of azodye were studied. Biosynthesis of stable copper oxide nanoparticles were preformed using Aloe barbadensis leaf extract as a size and shape-directing agent for preparing of spherical copper oxide nanoparticles by calcination at 400 °C. The leaf extract of Aloe barbadensis was prepared in deionized water. The produced copper particles were calcined at 400 °C to produce copper oxide nanoparticles. The characterization of copper oxide nanoparticles was performed by XRD, SEM, FTIR, UV spectroscopy. The XRD analysis showed that the average particle size was between 5-30 nm. The shape of the copper oxide nanoparticles was spherical and cubic. EDX of the synthesized nanoparticles showed the composition consists of 68 % copper. The UV-visible spectrum analysis confirms an absorption peak at 200-400 nm of the produced CuO nanoparticles. The FTIR analysis of the copper oxide particles indicate the presence of the organometallic Cu-O bond between Cu and O. The obtained copper oxide was used as a removing agent of Congo red dye. The effect of variables like concentration, time, pH, adsorbent dosage were studied. It was observed from the results that the maximum dye removal occurs at pH of 4, the concentration of nanoparticles was 1 mg/L and the maximum time for dye removal was 120 min. The Langmuir isotherm model was calculated to study the adsorption efficiency of the Congo red dye on the produced copper oxide nanoparticles. The kinetics of pseudo second order is followed by adsorption. The calculated sum of square was 0.012 and r2 = 0.98. it was observed that the model fit the best and straight line with r2 value of 0.991 and probability value of 1.6E-5. The nanoparticles remove 70 % of the Congo red dye from its solution. This showed that the copper oxide nanoparticles has efficient capacity of azodye degradation.

In vitro Antibacterial Activity of Organic Extracts of Aloe barbadensis against Multi-drug resistant Pseudomonas aeruginosa Isolated from Wound Specimens

Objectives: In order to investigate alternate therapeutic option, this study was carried out to assess the in vitro antibacterial activity of gel extract of Aloe barbadensis against multiple antibiotic resistant Pseudomonas aeruginosa isolated from wound specimens.
 Methods: A total of 180 different wound specimens collected in a hospital, were subjected to isolate and identify P. aeruginosa by cultural methods. Antibiotic susceptibility testing was done by Kirby- Bauer disc diffusion method to screen multidrug resistant isolates. A. barbadensis extracts were prepared using aqueous and organic solvents and their in vitro inhibitory action was evaluated by agar well diffusion methods.
 Results: Out of total, 38 (21.1%) of the wound specimens showed the occurrence of P. aeruginosa, among which 15 (39%) isolates were multi-drug resistant. Organic extracts of various concentrations (0.2 - 0.8 v/v %) inhibited 66.7% of MDR and all non-MDR (n = 23) P. aeruginosa with zone of inhibition ranging from 9.5 ±1.0 to 21.3 ± 2.2 mm but not by aqueous extract. A positive Pearson’s correlation (r=0.983) was found between antibacterial effect and concentrations of the extracts. The antibacterial activity of organic extracts was statistically associated with antibiotic resistance profile of the organism (p<0.05).
 Conclusion: Organic extracts of A. barbadensis revealed variable in vitro inhibitory action against both MDR and non-MDR P. aeruginosa isolated from wound specimens. Although further confirmation is needed, aloe gel extract may be applied as an alternate treatment option.

Protective effects of the aqueous leaf extract of Aloe barbadensis on gentamicin and cisplatin–induced nephrotoxic rats

ObjectiveTo study the protective effects of the aqueous leaf extract of Aloe barbadensis(AEAB) on gentamicin and Cisplatin–induced nephrotoxic Wistar rats. MethodsIn each model of nephrotoxicity, thirty adult male Wistar rats were evenly divided into 5 groups. Groups I and II served as untreated and model controls, respectively while groups III-V were the treatment groups which were pretreated with 100-200 mg/kg bodyweight per day of AEAB 1 h before each dose of the nephrotoxicants. On the 8th day(in case of gentamicin) and on 6th day(in case of Cisplatin), blood samples for serum urea, total protein and creatinine as well as some ions like sodium, potassium, chloride and uric acid while the rat kidneys for histology were obtained under inhaled diethyl ether anesthesia. ResultsIn the gentamicin nephrotoxic rats, 100-200 mg/kg bodyweight per day significantly attenuated elevations in the serum creatinine, total protein and blood urea nitrogen levels in dose related fashion and no treatment related effect on uric acid and ions, and attenuated the gentamicin-induced tubulonephrosis. Similar effects were also recorded in the Cisplatin model of acute renal injury. ConclusionsThe nephroprotective effect of AEAB could be due to the inherent antioxidant and free-radical-scavenging principle(s) contained in the extract. In the near future, AEAB could constitute a lead to discovery of a novel drug for the treatment of drug-induced nephrotoxicity.

Kidney and Liver Function Parameters in Alloxan-Induced Diabetic Rats Treated with Aloe Barbadensis Juice Extract

Aloe barbadensis juice extract has been reported to possess hypoglycaemic property but the effects of its use on kidney and liver functions in diabetic animals have not been well investigated. This study investigated some biochemical parameters in the liver and kidney of alloxan-induced diabetic rats treated with Aloe barbadensis juice extract. Alloxan-induced diabetic rats were administered orally with Aloe barbadensis juice extract for seven days after which some biochemical indices in the serum, liver and kidney were measured and compared with the control. Serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and creatinine of untreated diabetic group and treated diabetic groups were significantly elevated when compared with the normal control group with no significant changes in the levels of the enzymes in the liver and kidney. There were no significant changes (p>0.05) in the serum levels of Na+ and K+ in untreated diabetic group and treated diabetic groups when compared with the normal control rats which are not diabetic. These results suggest that administration of aqueous extract of Aloe barbadensis to diabetic rats did not have any adverse effect on the liver and kidney functions in rats showing that the extract is not toxic to man. Keywords: Aloe barbadensis, Alloxan, Liver function, Kidney function, Diabetic rats.

Antibacterial Activities of Crude Extract of Aloe barbadensis to Clinically Isolated Bacterial Pathogens

The antibacterial activity of Aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. Enterococcus bovis, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Morganella morganii, and Klebsiella pneumoniae causing infection in human being. Ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. Relatively higher MIC concentrations were obtained for gram negative bacteria E. coli and K. pneumoniae, with ethanol extract; however, no inhibitory effect was noted for aqueous extract. Ethanolic extract possesses great inhibitory activity for gram positive bacteria, E. bovis followed by S. aureus. Among gram negative bacteria, highest inhibitory effect was observed with P. aeruginosa, followed by M. morganii, P. mirabilis, and P. vulgaris, which was significant (p < 0.01) than E. coli and K. pneumoniae. Antimicrobial activity tests of crude extract of A. barbadensis were carried out to validate the use of traditional medicinal herbal and results of this study tend to give credence to the common use of A. barbadensis gel and leaf.

Prevention of the onset of hyperglycaemia by extracts of Aloe barbadensis in rabbits treated with alloxan

The ability of a home-made aqueous extract of Aloe barbadensis to prevent the onset of alloxaninduced hyperglycaemia was examined and compared with that of a factory-produced gel. Three groups of animals were administered 200 mg/kg body weight of alloxan intraperitoneally. A fourth group of animals was left uninjected. Animals in group 2 also received a uniform administration of 1 mg/ml twice daily oral intake of Aloe gel (the home-made extract) commenced at the same time the alloxan was administered and continued thereafter for the next six days. Animals in group 3 were administered the factory-produced extract in a similar way as was done for animals in group 2. Plasma glucose levels at the end of the experiment were 142.50±6.28, 82.50±2.72, 88.17±1.92 and 94.17±1.51mg/dl for animals in groups 1, 2, 3 and 4, respectively. Alloxan administration possibly resulted in an increase of 51% in the plasma glucose level of animals in group 1 relative to animals in group 4 (p

Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity

Aloe barbadensis Mill. Syn. Aloe vera Tourn. ex Linn.(Liliaceae) has been used in variety of diseases in traditional Indian system of medicine in India and its use for hepatic ailments is also documented. In the present study an attempt has been made to validate its hepatoprotective activity. The shade dried aerial parts of Aloe barbadensis were extracted with petroleum ether (AB-1), chloroform (AB-2) and methanol (AB-3). The plant marc was extracted with distilled water (AB-4). All the extracts were evaluated for hepatoprotective activity on limited test models as hexobarbitone sleep time, zoxazolamine paralysis time and marker biochemical parameters. AB-1 and AB-2 were observed to be devoid of any hepatoprotective activity. Out of two active extracts (AB-3 and AB-4), the most active AB-4 was studied in detail. AB-4 showed significant hepatoprotective activity against CCl 4 induced hepatotoxicity as evident by restoration of serum transaminases, alkaline phosphatase, bilirubin and triglycerides. Hepatoprotective potential was confirmed by the restoration of lipid peroxidation, glutathione, glucose-6-phosphatase and microsomal aniline hydroxylase and amidopyrine N-demethylase towards near normal. Histopathology of the liver tissue further supports the biochemical findings confirming the hepatoprotective potential of AB-4. The present study shows that the aqueous extract of Aloe barbadensis is significantly capable of restoring integrity of hepatocytes indicated by improvement in physiological parameters, excretory capacity (BSP retention) of hepatocytes and also by stimulation of bile flow secretion. AB-4 did not show any sign of toxicity up to oral dose of 2 g/kg in mice