Using hyperspectral imaging technology for rapid, non-destructive detection of soybean grain moisture content provides technical support for high-quality soybean harvesting. A total of 90 samples of soybean grains from different varieties were collected, with hyperspectral images acquired in the wavelength range of 900–1700 nm. The moisture content of each soybean grain sample was determined using the direct drying method as specified in GB 5009.3-2016. The samples were divided into a calibration set and a prediction set based on a 4:1 ratio using the sample partitioning method of Joint X-Y Distance. Eight preprocessing methods were applied to the raw spectral data, including baseline correction, moving average, Savitzky-Golay filtering, normalization, standard normal variate transformation, multiple scatter correction, first derivative, and deconvolution. Feature wavelengths were then extracted using the successive projections algorithm and the competitive adaptive reweighted sampling algorithm. Finally, a partial least squares regression model for predicting the moisture content of soybean grains was developed based on these feature wavelengths. The results show that the correlation coefficient and the root mean square error of the optimal model for the prediction set were 0.92 and 0.2371, respectively. The moisture spectrum inversion model can precisely and rapidly predict the moisture content of soybean grains non-destructively, thereby determining the timing of mechanical soybean harvesting and enhancing the quality of soybean harvesting, storage, and processing.
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