Algerian Varieties Research Articles

Polyphenolic Profile and in vitro Antioxidant Activity of Three Algerian Date (Phoenix dactylifera) Varieties

As known, dates are packed with antioxidants and bioactive compounds and provide various health benefits, as evidenced by their varying nitrite levels. Algerian dates, on the other hand, have not been thoroughly investigated for their bioactive compounds and overall antioxidant capacity. This research aims to tap into this potential by meticulously measuring total polyphenols, flavonoids, and condensed tannins in three popular Algerian varieties (Phoenix dactylifera L.) Ksiba, Hamraya, and Deglet Nour, and the determination of their antioxidant activity using (scavenging assays of 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing power, and total antioxidant capacity. The analysis showed that the three varieties of date fruits are rich in total phenolics with an amount ranging between 71±51 and 7975±389 mg of gallic acid equivalent (GAE).100 g-1 of dry weight (DW), the flavonoid amount ranged from 31±3 to 767±4 mg of quercetin equivalent (QE) 100 g-1 DW and condensed tannins between 6± 2 and 653 ±64 mg of catechin equivalent (CE) 100 g-1 dry DW. The antiradical activity was quite promising and ranged between 0.5 and 24 µg AAE mg-1 extract for DPPH and between 2 and 113 µg AAE mg-1 extract for ABTS, while the reducing power and total antioxidant capacity values ranged from 16 to 154 μg ascorbic acid (AAE) mg-1 and 39 to 68 μg AAE mg-1 extract respectively. The results of this study show that Algerian date fruit can be regarded as a potential natural source of antioxidants, with ethyl acetate serving as the best extractant solvent, resulting in higher polyphenol content and antioxidant activities.

Molecular analysis of olive (Olea europaea L.) in Iraq using the internal transcribed spacer (ITS) region

Olea is a genus of about 40 species in the Oleaceae family distributed in warm temperate and tropical areas of southern Europe, Africa, southern Asia, and Australia. The study aimed to use the variations in the Internal Transcribed Spacer 1 (ITS1) region to construct a phylogenetic tree for 12 Iraqi olive varieties with 15 ITS1 sequences downloaded from the NCBI database. Leaf samples were collected from 12 olive varieties grown in five geographical regions of the north of Iraq. Using the BLAST algorithm, 12 sequences were manually adjusted by chromatogram comparison and aligned with the NCBI GenBank database. Haplotype diversity ranged from zero in the Australian population to one in the Moroccan and Spanish populations. Nucleotide diversity ranged from 0.0 in the Australian population to 0.08 in the Algerian population and Tajima's D value was −0.940. Genetic differentiation values ranged from 0.0177 between Iraqi Moroccan and Iraqi Spanish populations to 0.6540 between Iraqi and Algerian populations. The average pairwise nucleotide diversity within populations was 0.9036 in Iraqi and Turkish olive populations to 0.8671 in Iraqi and Algerian populations. The phylogenetic tree formed 2 main clusters. The first cluster includes all Algerian varieties and the Iraqi varieties are grouped in second cluster. The negative Tajima test value in all populations indicates an excess of rare alleles. The high value of gene flow between the Iraqi and Australian populations may have been mediated by human transport and these varieties may have been originated from the same geographical region.

Comparative chemical composition and antioxidant activity of olive leaves Olea europaea L. of Tunisian and Algerian varieties

The main objective of this research was to understand the effect of different solvents (ethanol, ethyl acetate, and hexane) on the extraction of bioactive compounds in addition to the antioxidant activity of eight Tunisian and Algerian olive leaf (Olea europaea L) cultivars. The different extracts were evaluated for their content of total phenols, flavonoids, and condensed tannins. The antioxidant capacity was determined by ABTS (2,2-azinobis (3 ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1 picrylhydrazyl) methods. In addition, qualitative and quantitative analyses of phenolic compounds by HPLC were performed. In terms of phenolic content, ethanol was found to be the best solvent to extract phenolic compounds with antioxidant capacity. The ethanolic extract of Sigoise and Rougette varieties were the richest in total phenols 161.54 ± 0.99, and 160.53 ± 1.17 mg GAE/g DE respectively. The highest levels of flavonoids were detected in ethyl acetate extracts of Rougette (87.58 ± 6.99 mg QE/g DE) and Verdal varieties (86.77 ± 1.75 mg QE/g DE), whereas the hexanolic extracts of Gerboui, Sofiana, and Chemlali exhibited the higher amount of tannins (67.99 ± 5.41, 65.52 ± 1.94, 64.64 ± 1.51 mg CE/g DE, respectively). Furthermore, in the DPPH radical scavenging activity assay, the Sofiana extract exhibited the highest antioxidant activity (23.85 ± 0.58 mg/L). In another case, for the ABTS radical scavenging assay, the Verdal extract showed high antioxidant potential (30.22 ± 1.35 mg/L). HPLC analysis of ethanolic extracts of the different varieties allowed the identification of 19 organic compounds, with oleuropein (2604.01–7991.14 mg/100g DM) being the most abundant, followed by hydroxytyrosol (250.74–687.54 mg/100g DM) and verbascoside (216.12–672.64 mg/100g DM) being the major compounds in the olive leaves in all studied ethanolic extracts, with concentrations varying significantly according to the cultivars and geographical origin. Principal component analysis (PCA) performed on phenolic profiles and antioxidant activities revealed the influence of bioactive compounds on antioxidant activity, with high concentrations of phenols and flavonoids in olive leaf extracts having a strong antioxidant activity.

Classification of Algerian olive oils: Physicochemical properties, polyphenols and fatty acid composition combined with machine learning models

The regulation of olive cultivar and geographical origin is a requirement for the global extra virgin olive oil market, due to its significant impact on consumer choice. Our work involves obtaining a promising marker parameter for cultivar and geographical origin that can be used to verify declared labels. The effects of these factors on the physicochemical parameters and composition of monovarietal extra virgin olive oil (MEVOO) from Algeria were studied. Thirteen olive fruit varieties were analyzed using different physicochemical methods, including phenolic and fatty acid composition. Five classification techniques, random forests (RForest), gradient boosted trees (GBoost), Naïve Bayes (NBayes), logistic regression (LRegression) and decision tree (DTree), were applied and their results were compared. The best validation accuracy of 91.7 % was achieved with DT classification through a feature selection procedure using a genetic algorithm (GA). These results demonstrate the effective use of machine learning techniques to rapidly classify different Algerian varieties based on their compositional fingerprints.

Monitoring the maturity of Ahmeur Bouamer Autochtonous variety and determining of the optimum harvest date

Morphological ampelography characteristics, technological, phenolic and tannic maturities were studied over four seasons (from 2009 to 2012) of Ahmeur Bouamer autochtonous Algerian variety. During the four harvests, Ahmeur Bouamer's cluster has showed a high quality of morphological parameters (weight, size, number of seeds/ berries per cluster).The weight was proportional to the size of the cluster which was moderately compact, thus avoiding the deformation and the bursting of the berries. As For the maturity index (MI), Ahmeur Bouamer grape recorded values ranging from 31.07 to 54.95 for the four seasons; values which would make it possible to consider that the harvest would be of excellent quality (when 30 <MI <50). As well as for total anthocyanin potential (TAP) exceeding 1000 mg / L and total polyphenol index (TPI) with values greater than 50, these values confirm that the cluster presents high level of maturity. The monitoring of the MI every week in the phase of ripening (september-october) for 2010 and 2011 have showed that the maximum ripeness index was reached on September 27th with highest level of maturity for both harvests, date that can be considered by the winemaker as optimal date of harvest.

Comparative Study on Chemical Composition of Green and Black Table Olives Brines of the Endemic ''Sigoise'' Cultivar: Recovery of high-Added Values Compounds.

The effluents derived from the processing of table olives stand for a serious environmental problem. The study aims to valorize the brine water of table olives at different stages of ripening (green and black) of the Algerian variety Sigoise of Bejaia (East) and Mascara (West). The physico-chemical characterization revealed that these samples display have a high acid pH and salinity. The comparative study of phenolic levels exhibited showed very significant differences between the brine waters of green olives from Bejaia and Mascara, while the brines of black olives presented showed comparable levels. A high strong antioxidant potential was confirmed by DPPH (CI50 =0.35 μg/100 ml-0.50 μg/100 ml) and FRAP (CI50 =626.89 μg/100 ml-875.54 μg/100 ml) tests. Chemical screening by HPLC-DAD of the four samples identified high concentrations of hydroxytyrosol (HT) (390.4 mg/100 ml-360.8 mg/100 ml) and tyrosol (202.2 mg/100 ml-101.4 mg/100 ml). This study provided a deeper insight into the phenolic profile and the antioxidant potential of these brines.

HPLC-DAD profile of phenolic compounds and In vitro antioxidant activity of Ficus carica L. fruits from two Algerian varieties

Ficus carica L., commonly known as the fig tree, is a plant belonging to the Moraceae family whose fruits are traditionally used for edible and therapeutic purposes. The study aimed to investigate the lyophilized aqueous extracts of two native Algerian fig varieties, azendjar (Az) and taamriouth (Ta), as a potential source of antioxidant compounds for possible use as ingredients in pharmaceuticals or nutraceuticals. The HPLC-DAD analysis revealed the presence of two phenolic acids (3,4-dihydroxybenzoic acid and vanillic acid) and two flavonoids (rutin and quercetin) at levels 3.67, 4.80, 84.16, and 6.87 µg/g respectively for Az variety extract, and 6.90, traces, 7.46 and 3.37 µg/g respectively for Ta variety extract. Total phenolic content was determined using the Folin-Ciocalteu method at levels 951.06 ± 61.08 and 730.88 ± 45.25 GAE mg/100 g of the dry extract. In contrast, the total flavonoid content was determined using Christ-Müller's method at levels 428.34 ± 15.42 and 307.63 ± 7.94 QE mg/100 g of dry extract in the Az and Ta varieties, respectively. The total polyphenolic content of the extract may be responsible for its antioxidant action. The gathered results indicate that the extracts from the dark peel fig variety – azendjar, are characterized by a higher content of phenolic and flavonoid compounds and antioxidant activity than the extract from the light peel variety – taamriouth. In conclusion, the conducted studies and in vitro assays indicate that the studied extracts are a source of natural antioxidants and can be considered functional raw materials for producing food supplements and pharmaceuticals.

Exploring Olive Genetic Diversity in the Maltese Islands

A comprehensive effort was devoted to exploring, collecting and characterizing the local Maltese olive germplasm, often represented by ancient, monumental trees and by plants of uncertain origin. SSR and cp-SSR analysis of all samples enabled the identification of 46 genotypes and establishment of the correspondence between ancient trees, main local varieties and other Mediterranean cultivars. The application of plastid markers enabled identification of two lineages among Maltese genotypes, with more than 50% represented by lineage E2. Twenty-nine cases of grafting were identified among the various genotypes and lineages. In most cases, E1 canopies were grafted on E2 rootstocks, but reverse cases were also observed. The phylogenetic study of Maltese genotypes, together with hundreds of cultivars from the Mediterranean Basin and beyond, highlights the richness of Maltese olive diversity and drawing attention to the genetic similarity of some Maltese olive genotypes with neighboring Italian and Algerian varieties. These results underline the long-lasting presence of the olive in the country, contributing to the reconstruction of its phylogeny and demonstrating a possible autochthonous origin of many samples. Some still-living ancient trees are at serious risk of extinction due to abandonment, urban expansion and environmental threats. This study supports the preservation of the Maltese olive germplasm and highlights its importance as a rich genetic source to face new agronomical challenges and future climatic constraints.

Phytochemical, antioxidant, and antimicrobial attributes of different extracts of seeds: the Algerian variety of dates ‘Deglet Nour’ (Phoenix dactylifera L.)

Phytochemical, antioxidant, and antimicrobial attributes of different extracts of seeds: the Algerian variety of dates ‘Deglet Nour’ (Phoenix dactylifera L.)

TAJA Corpus: Linguistically Tagged Written Algerian Judeo-Arabic Corpus

Abstract The Tagged Algerian Judeo-Arabic (TAJA) corpus is the first linguistically annotated corpus of any Judeo-Arabic dialect regardless of geography and period. The corpus is a genre-diverse collection of written Modern Algerian Judeo-Arabic texts, encompassing translations of the Bible and of liturgical texts, commentaries and original Judeo-Arabic books and journals. The TAJA corpus was manually annotated with parts-of-speech (POS) tags and detailed morphology tags. The goal of the new corpus is twofold. First, it preserves this endangered Judeo-Arabic language, expanding on previous fieldwork and going beyond the study of individual written texts. The corpus has already enabled us to make strides towards a grammar of written Algerian Judeo-Arabic. Second, this tagged corpus serves as a foundation for the development of Judeo-Arabic-specific Natural Language Processing (NLP) tools, which allow automatic POS tagging and morphological annotation of large collections of yet untapped texts in Algerian Judeo-Arabic and other Judeo-Arabic varieties.

Biological activities and phenolic compounds of olive oil mill wastewater from Abani, endemic Algerian variety

The current study aimed to determination of cytotoxicity, antioxidant, anti-inflammatory, anti-hemolytic, and anticoagulant activities of phenolic compounds extracted from olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern in Algeria. The LC–MS (liquid chromatography–mass spectrometry) results were revealed the presence of 20 phenolic compounds in the extract of OMW and mostly consisted of Kaempferol, 4,5-di-O-caffeoyquinic acid, quinic acid, and caffeic acid. The extracts possessed effective reducing power (FRAP) and high radical scavenging activity against DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS + (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) free radicals, and it inhibited cytochrome c reduction in a dose-dependent manner. They exert a protective effect on red blood cells, and they were found to exhibit the highest inhibitory effect anti-inflammatory activity using inhibition of protein denaturation (IPD) and membrane stabilizing potential (MSP) tests (80.46 ± 3.81 µg/mL and 87.43 ± 0.66 µg/mL) more than the standard used. The extract also showed the greatest anticoagulant activity in both the endogenous and exogenous routes (44.77 ± 0.25 s and 15.84 ± 0.12 s, respectively). Based on these findings, it is reasonable to infer that OMW is a good source of natural phenolic compounds with potential antioxidant, anti-inflammatory, and anticoagulant properties.

Valorization of Pinus halepensis Mill. seed oil: Physicochemical characteristics, bioactive compounds, and antioxidant activity as affected by location and extraction method

In this study, physicochemical characteristics, phytochemical compositions, and antioxidant activities of Pinus halepensis Mill. seed oils (PHSO) from two geographical origins (Algeria and Turkey) and obtained through two different extraction processes (cold pressing and solvent extraction) were determined. PHSO were majorly consisted of linoleic and oleic acids. Cold pressing yielded oils with lower peroxide and totox values, higher tocopherols contents (309.42–318.04 mg/100 g), higher DPPH-radical scavenging activities (25.33–26.94 mM TE/kg), and reducing capacity values (301.30–329.94 mg GAE/kg). The solvent-extracted oils contained more phytosterols (5,683.25–5,964.20 mg/kg), particularly β-sitosterol and campesterol. Phenolic compounds of PHSO were identified by HPLC-DAD for the first time in this study. More phenolic compounds were detected in solvent-extracted oils. Turkish PHSO were richer in individual phenolics (18,928.92 μg/100 g) than the Algerian variety (2,821.01 μg/100 g). Different extraction techniques and geographical origins led to differences in physicochemical and phytochemical attributes of PHSO. Novelty impact statement This study revealed that extraction technique (cold-pressing, solvent extraction) and geographical seed origin (Algeria, Turkey) influenced the Pinus halepensis Mill. seed oils (PHSO) composition. PHSO are rich in fatty acids, sterols, and tocopherols majorly linoleic acid, γ-tocopherol, and β-sitosterol which were detected for first time for the cold pressed oils. This is the first report on phenolics compounds of these oils, mainly luteolin. The high oxidative stability and good antioxidant activities of PHSO promote their exploitation in food, cosmetics, and pharmaceuticals uses.

Citrus × paradisi essential oil as a promising agent for margarine storage stability: Composition and antioxidant capacity

Grapefruit (Citrus × paradisi, Algerian variety) essential oil obtained by hydro-distillation was evaluated as a substitute for vitamin E for margarine. The obtained IC50 values were: 39.92 ± 0.18, 15.09 ± 0.02, and 4.42 ± 0.03 mg/ml by the 2,2-diphenyl-1-picrylhydrazyl method, the reducing power and the β-carotene/linoleic acid tests, respectively. These results were higher compared to those of the synthetic antioxidants. Values for BHA in DPPH and -carotene/linoleic acid assays were 0.158 ± 0.003 and 0.032 ± 0.000 mg/ml, respectively. The result of ascorbic acid for reducing power was 0.053 ± 0.001 mg/ml. Analysis of composition showed 128 volatile compounds. The limonene was the major compound (67.22%) followed by trans-citral (5.39%) and isoauraptene (2.85%). All samples were fortified with 12.5, 50 and 100 µg/g of essential oil. The Rancimat test demonstrated that the lowest concentration of essential oil gave the best resistance to oxidation (22.6 hr). Novelty impact statement Grapefruit essential oil was used as a substitute for vitamin E for margarine, and it gave better resistance to oxidation than control samples. The presented solution could be successfully applied on an industrial scale.

First morphological characterization of autochthonous olive (Olea europaea L.) denominations from central and eastern of Algeria

Olive (Olea europaea L.) resources in Algeria are very little investigated. In fact, a total of 60 denominations have been the subject of characterization studies and they are cited in the bibliography but only 36 varieties are described and listed in the catalog of Algerian varieties of olive tree. In this work, a study on the diversity by mean of a field survey followed by morphological characterization, an estimate of the Shannon diversity index of a set of denominations collected in central and eastern of Algeria were carried out. The survey allowed us to note the existence of 33 denominations never mentioned in the bibliography. Morphological characterization based on the characteristics of tree, fruit and endocarp allowed us to a morphological description of 23 denominations. The Principal Component Analysis, Multiple Correspondence Analysis and Ascending Hierarchical Classification analysis allowed us to classify them into a few groups. The value of the relative diversity of all denominations is slightly below the average (0.42). The results obtained in this work provide very useful information on certain morphological characteristics of the studied denominations and indicate the critical conditions in which several denominations are found, which constitutes a great risk of genetic erosion.

Molecular Analysis of the Official Algerian Olive Collection Highlighted a Hotspot of Biodiversity in the Central Mediterranean Basin.

Genetic diversity and population structure studies of local olive germplasm are important to safeguard biodiversity, for genetic resources management and to improve the knowledge on the distribution and evolution patterns of this species. In the present study Algerian olive germplasm was characterized using 16 nuclear (nuSSR) and six chloroplast (cpSSR) microsatellites. Algerian varieties, collected from the National Olive Germplasm Repository (ITAFV), 10 of which had never been genotyped before, were analyzed. Our results highlighted the presence of an exclusive genetic core represented by 13 cultivars located in a mountainous area in the North-East of Algeria, named Little Kabylie. Comparison with published datasets, representative of the Mediterranean genetic background, revealed that the most Algerian varieties showed affinity with Central and Eastern Mediterranean cultivars. Interestingly, cpSSR phylogenetic analysis supported results from nuSSRs, highlighting similarities between Algerian germplasm and wild olives from Greece, Italy, Spain and Morocco. This study sheds light on the genetic relationship of Algerian and Mediterranean olive germplasm suggesting possible events of secondary domestication and/or crossing and hybridization across the Mediterranean area. Our findings revealed a distinctive genetic background for cultivars from Little Kabylie and support the increasing awareness that North Africa represents a hotspot of diversity for crop varieties and crop wild relative species.

Algerian cardoon flowers express a large spectrum of coagulant enzymes with potential applications in cheesemaking

Proteases from flowers of Algerian cultivated and wild cardoons were purified, characterised and compared with those purified from flowers of a Portuguese variety. Three cardosins (A0, A and B) were obtained from each variety. All of them were dimeric and comprised heavy and light chain. Cardosins from Algerian varieties presented higher molecular masses and were less glycosylated than their Portuguese counterparts. Milk coagulation and curd yield parameters revealed a large difference between cardosins A0, A and B and among the same cardosin from different origins. The enzymatic specificity of cardosins, studied against β-chain of oxidised insulin and κ-casein, showed no prevalent effect of varieties. However, compared with cardosins A0 and A, cardosin B was more proteolytic and led to more complex digestion profiles. The present study reports the first description of the diversity of cardosins in Algerian cardoon flowers and sustains their potential use as milk coagulants for cheesemaking.

MINERAL NUTRIENT STATUS OF SOME MEDITERRANEAN BARLEY VARIETIES AS AFFECTED BY DROUGHT STRESS IN EGYPT

Field experiment was carried out at the experimental research station of Nubaria, representing the newly reclaimed sandy loam soils of Egypt. The study was done to evaluate the nutrient status of barley varieties (Egyptian, Tunisian, Algerian and Morocco) grown under normal (75% water holding capacity=WHC) and stress condition (40% WHC). Results indicated that the Egyptian variety El-Arish scored the highest values of N% under normal and drought stress condition and Giza 127 for P and Giza 123 for K in same sequence. The lowest values of N, P and Ca content observed with Giza 2000 variety. As for the Tunisian varieties, the lowest reduction percentage was attained at Raihane (9.38%, 3.54% and 3.62%) for N, K and Ca, respectively. Algerian barley varieties, N in Naïlia/Techedrett, P in Temacine/Ksar Megrine, K in Ras El-Mouche/Temacine, Ca in Saida/Ksar Megrine, got the highest and lowest values under normal and stress conditions, respectively. Morocco varieties, lowest values of N, P, K and Ca were recorded for Massine, Taffa and Amalou under water stress condition. The highest and lowest value of Fe, Zn and Mn were recorded for barley varieties Giza 2000, Giza 131 and Giza 127, respectively. Results concluded that the Egyptian varieties (Giza 127, Giza131, Giza 2000), Tunisian varieties (Kebili-3 and Tozeur-2), Algerian varieties (Techedrett, Naïlia) and Morocco varieties (Laanaceur, Amira) registered the lowest reduction percentage in nutrient content indicating their tolerance to sustain its productivity under water stress condition.

Manufacture of a Coffee Substitute from Date Kernels of the Algerian Variety Mech-Degla

The present study has two components, in the first place the morphological and physicochemical characteristics of the inedible part of the date (kernels) have been determined, in order to bring additional knowledge on these characteristics, which helps to highlight the possibility of their valuations. The dimensions of the date kernels are between (0.9021 to 1.2829g) for the weight, (1.9 to 2.5cm) for the length and (0, 5 to 0.8cm) with an average of 0.6cm for the width of the core. An acidic pH of 4.55, and a moisture content of 6.38% has 0.8% ash content and a crude fiber content of 76.37%, a total sugar content of 4.69%, a crude protein content of 5.42% a fat content of 15.9% and the pulp / core ratio is 4.79. The second part deals with the valorization of the “Mech-Degla” date kernels in the manufacture of a coffee substitute. A sensory evaluation of color, smell, flavor and general appreciation of the date kernel coffee substitute was performed. The production of a date-kernel-based coffee substitute is one of the processes for exploiting the by-products of date processing. Statistical analysis of the sensory evaluation results of color, odor, flavor,and general acceptability reveals that although this is the first time for most tasters to taste a coffee substitute at core dates, but they showed great acceptability towards this new product especially when they added sugar (sweetener).

Phytochemical screening, antioxidant and antimicrobial activities of grape (Vitis vinifera L.) seed extracts from red globe and Valenci Algerian varieties

Until now, there is no documentation concerning the composition, biological and pharmaceutical activities of Algerian grape (Vitis vinifera L.) seeds. The present study aims to evaluate the phytochemical analysis, antioxidant and antimicrobial activities of grape seed extracts (GSEs) from the Algerian Red globe and Valenci varieties. The total polyphenols content (TPC) in the GSEs was evaluated by spectrophotometry, it was 398.01 ? 18.12 mg GAE/g GSEs and 335.11 ? 11.44 mg GAE/g GSEs for the Red globe and Valenci varieties respectively. The antioxidant activity of GSEs was also evaluated by spectrophotometry on stable free radicals of DPPH. The IC50 for the Red globe variety was 4 ? 0.2 ?g/ml, while for the Valenci variety it was 4.6 ? 0.36 ?g/ml. The antimicrobial activity was determined using the disk diffusion method. Both extracts showed antimicrobial activity against the eight studied strains: Escherichia coli, Pseudomonas aeruginosa, Pasteurella sp., Salmonella sp., Staphylococcus aureus, Streptococcus faecalis, Clostridium sp., and Candida albicans. The extracts produced a clear inhibition zone for all the tested strains. Inhibition diameters greater than 19 mm were registered. The obtained results in this study indicate the differences between the Red globe and Valenci varieties, in terms of polyphenol content, antioxidant and antimicrobial activities in Algerian GSEs.

Genetic identification, origin and sanitary status of grapevine cultivars (Vitis vinifera L.) grown in Babar, Algeria.

This research focused on present grapevine biodiversity of neglected cultivars grown in 'Babar' region, Northeastern Algeria. The obtained results demonstrate the complex, rich, and even surprising inheritance of grapevine biodiversity in such a small region, with currently residual viticulture practiced only for direct consumption. Babar is one of the oldest inhabited areas in Algeria and part of the Atlas Mountains, considered very favorable for wild and cultivated vine growing since protohistoric times. Thirty-seven vines from the traditional growing area were analyzed using nuclear microsatellite (SSR) markers for cultivar identification and RT-qPCR analysis for virus detection and sanitary status evaluation. As a result, thirteen different genotypes were found, most of them showing a very good sanitary status, then constituting a valuable biological source for clonal selection. A close relatedness was evidenced with some Mediterranean varieties, resulting from previous exchanges of grapevine cultivars in the past. Furthermore, the present study highlighted the existence of three new genotypes, highly probably autochthonous of Babar region, with proposed names 'Babari', 'Babar-Algeria', and 'Amesski-Babar'. They could represent unique Algerian varieties, probably preserved over time. The conservation of these endangered genotypes is highly recommended.