Algerian Cattle Research Articles

Molecular characterization of Hyalomma scupense and its vector-borne pathogen Theileria annulata in Ksar El Boukhari (Medea, Algeria)

This study analyzed the molecular and phylogenetic profiles of Theileria annulata, the causative agent of tropical theileriosis in cattle, and its tick vector Hyalomma scupense in Algeria. Forty H. scupense ticks were collected in Medea, with 5 testing positive for Theileria spp. based on partial COXIII gene sequences. Positive ticks were further analyzed using COX1 and 12S rRNA genes. Two novel H. scupense 12S rRNA haplotypes and one novel COX1 haplotype were identified. One T. annulata haplotype previously reported in Algerian cattle was detected. This represents the first molecular characterization of T. annulata from H. scupense ticks in Algeria, providing insights into the genetic diversity of the parasite vector in this region. Overall, the study reveals new haplotypes for both the tick vector and parasite, furthering our understanding of their molecular profiles and phylogenetics in Algeria.

Allele and genotype frequencies of β-lactoglobulin gene using PCR-RFLP in Algerian local cattle populations.

Milk protein genetic polymorphisms are associated with economically important traits in dairy cattle. The objective of this study is to genotype a single nucleotide polymorphism (SNP) responsible for the amino acid changes in the beta-lactoglobulin (β-Lg) variants A and B on 85 unrelated DNA representing Algerian cattle populations: Chelifienne (28), Cheurfa (31) and Guelmoise (26). The method used is the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Genetic polymorphism was detected by digestion of PCR products amplified of exon II of β-Lg gene by with the endonuclease HaeIII enzyme. The results revealed that the amplified product was observed as 247 bp. Restriction digestion with HaeIII revealed three genotypes: AA, AB and BB. The genotypic frequencies of AA, AB and BB genotypes were 0.08, 0.41, 0.50; 0.08, 0.41, 0.50 and 0.01, 0.19, 0.56 in Chelifienne, Cheurfa and Guelmoise and respectively. Frequency of AA genotype was absent in Guelmoise population. Frequencies of A and B alleles were 0.29 and 0.71 in both Chelifienne and Cheurfa and 0.25 and 0.75 Guelmoise population. These results further confirm that Bos torus cattle are predominantly of β-Lactoglobulin B type. The Chi-square test at p-value < 0.05 results revealed that the Chelifienne and Cheurfa populations were in Hardy-Weinberg equilibrium and the results are not significant for the Guelmoise. This genetic information could be useful to estimate the effect of polymorphism on different milk production of Algerian bovine populations.

Milk quality and production under climate change uncertainty: case of the Algerian cattle breed

Algerian indigenous cattle breeds are well adapted to the harsh local arid and semi-arid environments. This study aims to summarize livestock practices, milk quality, and discuss the potential of local cattle breeds to maintain production capacity in the face of global warming conditions. A total of 175 smallholder farmers who practice the breeding of the Algerian local cattle breed were interviewed using a formal questionnaire. Following that, 122 milk samples were collected for physicochemical and bacteriological analyses. Climate data variability in the study area was evaluated. Results reveal that between 1980 and 2018, the average annual temperature rose by 0.3 ± 0.001 °C per year. Predictions suggest that by 2081 to 2100, temperatures could increase by 1.18°C under SSP1-2.6, 2.33°C under SSP2-4.5, and 4.59°C under SSP5-8.5. In the same period from 1980 to 2018, annual precipitation decreased by -0.99 ± 0.24 mm per year. Projections indicate a further decline of 22.5 mm for SSP1-2.6, 44.4 mm for SSP2-4.5, and 95.2 mm for SSP5-8.5 from 1980-2000 to 2081-2100. These changes in temperature and precipitation coincided with an expansion of cropland, which increased by 90.3% from 1992 to 2005. Conversely, pasture areas decreased by 53.7% between 1993 and 2009. A socio-demographic survey revealed that breeders have a low educational level (39.4% are unlettered). They own a small herd (6.84 ± 8.66 cattle). Moreover, the average daily milk production was 4.13 ± 2.12 Liters/cow, with acceptable physicochemical quality but poor bacteriological quality. Considering the climate change vulnerability of the study area, we can conclude that the exploitation of local breeds seems to be the best adaptation strategy to climate change effects. Conservation programs for local breeds can enhance biodiversity and ecosystem balance. Concurrently, genetic improvement programs have the potential to boost productivity and profitability, making substantial contributions to social equity and local economies.

STUDY OF FACTORS INFLUENCING NOTIFICATION OF BOVINE ABORTIONS BY PRACTICING VETERINARIANS ON DAIRY CATTLE FARMS IN EASTERN ALGERIA.

Bovine abortion is one of the most important causes of economic losses in Algerian cattle farms and industry. A descriptive epidemiological survey was conducted from November 2021 to November 2022 in eastern Algeria to quantify the extent of this scourge and to evaluate the risk factors for reporting abortions using a questionnaire intended for 150 practising veterinarians randomly distributed in five wilayas of eastern Algeria. The statistical analysis of the different risk factors was carried out using a univariate and then multivariate logistic regression model. The results obtained showed that 83.3% of the veterinarians audited had had an abortion rate of more than 5% during the previous 12 months. In the multivariate logistic regression analysis, one risk factor and one protective factor were identified. Indeed, all the areas studied had a very high risk of bovine abortion cases: Batna (odds ratio [OR] = 24.53; 95% confidence interval [CI]: 1.39 - 278.66), Sétif (OR= 73.08; 95% CI: 4.37-669.68), Mila (OR= 245.22; CI: 18.51 - 2306.71) and Bordj Bou Arreridj (OR= 49.09; CI: 3.57-738.69). However, the summer season (OR= 0.23; 95% CI: 0.06 - 0.48) was considered a protective factor, designated as the period of least reporting of abortions by the audited veterinarians compared to winter and autumn.

Description of the zootechnical performance of the indigenous Algerian cattle breeds “Atlas Brown” from the region of El Ouldja in western Algeria

Description of the zootechnical performance of the indigenous Algerian cattle breeds “Atlas Brown” from the region of El Ouldja in western Algeria

Pseudochrobactrum algeriensis sp. nov., isolated from lymph nodes of Algerian cattle.

Three Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile strains (C130915_07T, C150915_16 and C150915_17) were isolated from lymph nodes of Algerian cows. On the basis of 16S rRNA gene and whole genome similarities, the isolates were almost identical and clearly grouped in the genus Pseudochrobactrum. This allocation was confirmed by the analysis of fatty acids (C19:cyclo, C18 : 1, C18 : 0, C16 : 1 and C16 : 0) and of polar lipids (major components: phosphatidylethanolamine, ornithine-lipids, phosphatidylglycerol, cardiolipin and phosphatidylcholine, plus moderate amounts of phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and other aminolipids). Genomic, physiological and biochemical data differentiated these isolates from previously described Pseudochrobactrum species in DNA relatedness, carbon assimilation pattern and growth temperature range. Thus, these organisms represent a novel species of the genus Pseudochrobactrum, for which the name Pseudochrobactrum algeriensis sp. nov. is proposed (type strain C130915_07T=CECT30232T=LMG 32378T).

Evaluation of the discriminatory power of spoligotyping and 19-locus mycobacterial interspersed repetitive unit-variable number of tandem repeat analysis (MIRU-VNTR) of Mycobacterium bovis strains isolated from cattle in Algeria.

Bovine tuberculosis (bTB) caused by Mycobacterium (M.) bovis and M. caprae is a transmissible disease of livestock, notifiable to the World Organization for Animal Health (OIE). BTB particularly affects cattle and small ruminants and can be transmitted to humans thereby posing a significant threat to veterinary and public health worldwide. M. bovis is the principal cause of bTB in Algeria. In order to better understand the route of spreading and elaborate an eradication program, isolation and characterization of mycobacteria from Algerian cattle was performed. Sixty strains belonging to the M. tuberculosis complex were analyzed by spoligotyping, thereof 42 by 19-locus-MIRU-VNTR-typing. Spoligotyping revealed 16 distinguishable patterns (Hunter-Gaston discriminatory index [HGDI] of 0.8294), with types SB0120 (n = 20) and SB0121 (n = 13) being the most frequent patterns, representing 55% of the strains. Analyses based on 19-locus-MIRU-VNTR yielded 32 different profiles, five clusters and one orphan pattern, showing higher discriminatory power (HGDI = 0.9779) than spoligotyping. Seven VNTR-loci [VNTR 577 (alias ETR C), 2163b (QU11b), 2165 (ETR A), 2461 (ETR B), 3007 (MIRU 27), 2163a (QUB11a) and 3232 (QUB 3232)] were the most discriminative loci (HGDI ˃ 0.50). In conclusion, 19-locus-MIRU-VNTR yielded more information than spoligotyping concerning molecular differentiation of strains and better supports the elucidation of transmission routes of M. bovis between Algerian cattle herds.

Genetic characterization of four Algerian cattle breeds using microsatellite markers

Cattle plays a very important role in agriculture and food security in Algeria. In the present study, the genetic diversity and structure of Algerian indigenous cattle populations were evaluated by microsatellite markers. A total of 138 individuals belonging to four cattle breed populations were characterized using 22 microsatellite markers. A total of 360 alleles was detected across studied all loci. Results obtained for the mean number of alleles (16.36), expected heterozygosity (0.84) and polymorphic information content (0.82) indicated that the total analyzed populations are characterized by noticeable genetic variability. It can be said that there is a low genetic differentiation in the cattle populations studied considering obtained mean FST value (0.039). It was revealed 97.10% of the total genetic variation can be explained by genetic differences among individuals while 2.90% among populations. The structure, factorial correspondence analysis results and dendrogram showed that cattle populations studied are clustered in three groups. The present study has revealed an important knowledge about the genetic diversity and the relationship between some native cattle breeds raised in Algeria. The results showed that the breeds studied have a high genetic diversity. Moreover, it can be said that microsatellite markers used can be successfully used to determine genetic diversity and population structure in Algerian cattle breeds.

New insight into genetic variation and haplotype diversity of Fasciola hepatica from Algeria.

The liver fluke Fasciola hepatica is the main cause of fasciolosis in North Africa leading to significant economic losses and public health problems. In this study, the ribosomal internal transcribed spacer (ITS), cytochrome c oxidase I (COI), the mitochondrial region spanning the COI-trnT-rrnL, and the NADH dehydrogenase subunit I (NADI) markers were used to characterize Fasciola flukes from Algeria. Fasciola appeared widespread from the east to the west of Algeria. Among 1701 sampled cattle from 8 Algerian provinces, 5% were infected. Using morphological and morphometric analysis, one morphotype of Fasciola was observed. Nuclear ITS marker indicated that all collected flukes belong to F. hepatica. Multiple alignments of ITS dataset revealed two haplotypes, one described here for the first time. Analysis of molecular variance (AMOVA) of mitochondrial markers revealed weak population structure in Algeria. Mismatch distributions, neutrality tests, and median-joining network analysis all were compatible with a recent expansion of Algerian F. hepatica population. Fasciolosis appeared common in Algerian cattle, it seems that the absence of control strategy coupled to the favorable Mediterranean climate may lead to a reconstruction and dispersion of its populations. This study provides important results concerning the genetic characterization and variability of F. hepatica in Algeria as well as the significant role of cattle importation in shaping its dispersal route worldwide.

Genetic diversity and relationships among six local cattle populations in semi-arid areas assessed by a bovine medium-density single nucleotide polymorphism data

The local cattle populations belonging to the ‘Brune de l’Atlas’ cattle in Algeria and Morocco are potential resources in terms of genetic diversity and socioeconomic prevalence and their characterization is an essential step in any program designed to conserve genetic diversity. Our objectives were to assess the genetic diversity, the population structure and relationships among four Algerian cattle breeds, the Biskra, Cheurfa, Chelifienne and Guelmoise and of two Moroccan, the Oulmès-Zaër and Tidili by genotyping 50 309 single nucleotide polymorphism in 203 unrelated animals. A low population structure was observed across breeds with pairwise F ST values ranging from 0.008 to 0.043, suggesting a high level of gene flow. These data were combined with the available data on cattle populations representative of Europe (EUT), West African taurine (WAT) and zebu (ZEB). Principle Components Analysis was carried out which revealed that the Maghrebin populations are closer to the EUT/ZEB population than to the WAT. Structure analysis confirmed this mixed origin of the Maghrebin cattle populations. We also detected the influence of zebu breeds in Cheurfa and Guelmoise populations. This study provides the first information about genetic diversity within and between Algerian and Moroccan cattle populations and gives a detailed description of their genetic structure and relationships according to their historical origins. This study revealed that several combined effects contributed to shape the genetic diversity of the six Maghrebin populations studied: (i) gene flow among local breeds, (ii) the recent introgression of European breeds in local Algerian breeds and (iii) the traditional management systems. The results of this study will primarily assist policy makers and livestock keepers to make useful decisions for improvement of genetic resources while ensuring the preservation and conservation of local breeds in Algeria and Morocco.

The characterization of Brucella strains isolated from cattle in Algeria reveals the existence of a B. abortus lineage distinct from European and Sub-Saharan Africa strains

Brucellosis is a zoonosis caused by bacteria of the genus Brucella that causes important economic losses and human suffering worldwide. Brucellosis control requires an understanding of the Brucella species circulating in livestock and humans and, although prevalent in African countries of the Mediterranean basin, data for this area are mostly restricted to isolates obtained from humans and small ruminants. Here, we report the characterization of twenty-four Brucella strains isolated from Algerian cattle. Bruce-ladder multiplex PCR and conventional biotyping showed that Algerian cattle are infected mostly by B. abortus biovar 3, and to less extent by B. abortus biovar 1 and B. melitensis biovar 3. Extended AMOS-ERY PCR showed that all Algerian B. abortus biovar 3 strains were of the subgroup 3b. Although by multi locus variable number of tandem repeats analysis (MLVA) most isolates were closer to the European counterparts, five strains displayed characteristics distinct from the European isolates and those of countries across the Sahara, including three repetitions of marker Bruce55. These five strains, plus an earlier isolate from an Algerian human patient, may represent a lineage close to clades previously described in Africa. These data provide the basis for additional molecular epidemiology studies in northern Africa and indicate that further bacteriological and molecular investigations are necessary for a complete understanding of the epidemiology of cattle brucellosis in countries north and south of the Sahara.

Molecular survey and genetic characterization of Anaplasma centrale, A. marginale and A. bovis in cattle from Algeria.

Bovine anaplasmosis could be caused by several Anaplasma species. The causative agents are transmitted by ticks and haematophagous arthropods with a high impact on both human and animal health. This study was conducted to estimate the infection rate and to characterize Anaplasma spp. in cattle from Algeria. A molecular survey was performed in Setif district (Northeast Algeria) where a total number of 180 cattle blood samples were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes. PCRs revealed that the infection rates of Anaplasma spp., Anaplasma centrale, Anaplasma marginale and Anaplasma bovis were 42.2%; 39.4%; 11.1% and 4.4%, respectively. All tested animals were negative for A. phagocytophilum. Co-infection occurred in 10% (18/180) of the tested animals, and the most common co-infection pattern was an association between A. centrale and A. marginale (5.5%). Five cattle (2.7%) were co-infected by the three Anaplasma species. Holstein animals (58.1%) were more infected by A. centrale than the other breeds (p=.01). The molecular prevalence of A. centrale was significantly higher in males (54.2%) than in females (34.1%) (p=.001). A. marginale msp4 genetic analysis indicated a high sequence diversity of Algerian strains, suggesting the importation of live cattle from different origins. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. centrale revealed a low degree of genetic diversity. Our study suggests that different species of Anaplasma are simultaneously present in the Algerian cattle. To the best of our knowledge, this is the first molecular study and genetic characterization of Anaplasma spp. in Algerian cattle.

The epidemiology of cattle abortion in Algeria.

In Algeria, the epidemiology of cattle abortions is not well understood. Therefore, the present study aims to estimate the prevalence of abortion in 75 Algerian cattle herds and correlate its possible association with brucellosis positivity and some managerial risk factors. The cattle abortion herd prevalence was 41.33% [95% CI 30.16-52.5%]. As for brucellosis, the serological evidence of brucellosis exposure was observed in 9 out of 75 herds accounting for 12% [95% CI 4.65-19.35] herd seroprevalence. The risk factor analysis using the univariable analysis followed by multivariable logistic regression did confirm that brucellosis positivity (OR=5.19), mixed herd (OR=2.5), contact with other herd (OR=2.91), presence of dog in the herd (OR=2.89), imported cattle (OR=1.91), and farmers with less than 2years' experience (OR=2.69) as risk factors for abortion in Algerian cattle herds. Targeting these factors using a comprehensive control measure is needed to improve animal welfare and reduce economic losses associated with abortion in dairy cattle.

Prevalence of The Main Infectious Causes of Abortion in Dairy Cattle in Algeria.

IntroductionAbortion in cattle is a major source of economic losses for the agriculture sector. It can be due to infectious or non-infectious factors. Among infectious factors, parasites, bacteria, viruses, and fungi can be involved. The present work investigated the prevalence of the main infectious agents of abortion in Algerian cattle.Material and MethodsAltogether 278 non-aborting and 82 aborting cows were analysed.ResultsThe prevalence ranged from 0% for Tritrichomonas foetus to 15% for Neospora caninum. Additionally, a case-control study was performed to find the association between the presence of the pathogens and the occurrence of abortion in cows. The odds ratios were significant for Neospora caninum, bovine herpes virus 4, BVD virus, Brucella abortus, Salmonella Dublin, Leptospira interrogans serovar Hardjo, and Coxiella burnetii.ConclusionsThe pathogens enumerated here could be major causes of abortion among Algerian cattle.

English

Molecular tools have become an increasingly important part of studying the epidemiology of infectious agents. These tools have allowed the aetiological agent within a population to be diagnosed rapidly with a greater degree of efficiency and accuracy than conventional diagnostic tools. They have enhanced understanding into the pathogenicity and virulence of the aetiological agent and subsequent deployment of appropriate control strategies. This paper reviews the contribution of molecular epidemiology to the diagnosis and control of some animal diseases such as foot and mouth disease (FMD), buetongue and peste des petites ruminants (PPR) in Algeria. Molecular epidemiology has helped in the characterization of FMDV type O circulating in Algerian cattle in 1999 and in 2014; in 1999, the sequencing analysis showed that the Algerian viruses belong to the West-African topotype with 99% similarity to a strain isolated in Cote d'Ivoire. In 2014, the virus was identified as 0/ME/SA/Ind-2001d lineage which was 99.69% identical to the field strains isolated from earlier Tunisian outbreaks. In a related development, two episodes of bluetongue outbreaks were reported in Algeria; the first with serotype II in 2000 that showed no significant difference with the Tunisian strain reported two months earlier and the second episode involving serotype I epidemiologically linked to South Africa (with 94.3% not similarity) indicating an origin from sub-Saharan Africa. Molecular techniques have also described the PPRV strain implicated in an outbreak in Ghardaia district, in the centre of Algeria. The strain clustered with lineage IV of PPRV and shared 97 to 99% similarity with the strain implicated in neighboring Morocco and Tunisia. Key words: Algeria, control, diagnosis, molecular epidemiology, Foot and Mouth Disease (FMD), bluetongue (BT), Peste des Petites Ruminants (PPR).

Comparison of different serological methods to detect antibodies specific to Neospora caninum in bovine and canine sera

Neospora caninum is an apicomplexan parasite responsible for paresis in dogs and abortion in cattle worldwide. Dogs serve as a definitive host, while cattle serve as intermediate host. Many different methods have been developed to detect specific antibodies present in cattle and dog serum. In the present study, the dense granule protein NcGRA6 was incorporated in a latex beads agglutination test (LAT), and compared to other serological methods, including enzyme-linked immunosorbent assay, the direct agglutination test, the immunoblot, and the indirect fluorescent antibody test (IFAT). Using the IFAT as the reference method, 100 sera isolated from Algerian cattle and 100 sera isolated from Algerian dogs, both possibly infected with N. caninum, were used to evaluate the LAT. The sensitivity, specificity, and kappa index were calculated for each host species and assay. For dog sera, the sensitivity and the specificity of the LAT was 76% and 100%, respectively. The McNemar test showed that the LAT was not significantly different from IFAT (P > 0.05). For cattle sera, the sensitivity and the specificity of the LAT were 60% and 100%, respectively. The McNemar test indicated that the LAT was significantly different from IFAT (P < 0.01) and that the LAT was only positive for cattle sera with titers of 1:800 or greater, indicating that LAT can be used for cattle in a clinical context. As well, the LAT has the advantage of being easy and rapid to perform compared to the other assays.

Study of the risk factors associated with Neospora caninum seroprevalence in Algerian cattle populations

Bovine abortions due to Neospora caninum infection were reported worldwide. The situation in Algeria was unknown. For the evaluation of the prevalence of N. caninum and its associated risk factors, 799 cattle belonging to 87 farms of the north and northeast of Algeria were analyzed. The cattle were divided into imported cattle, local cattle and improved cattle corresponding to breeding between imported and local cattle. Sera were examined for the presence of N. caninum antibodies by indirect fluorescence antibody test. The overall seroprevalence for the 87 farms was 52.87% (41.28–62.71%). The overall animal seroprevalence was 19.64% (16.82–22.45%). The seroprevalence of N. caninum in local cattle (34.28%) was significantly higher (p<0.05) than in modern (16.04%) and improved (18.64%) cattle. The risk factors analysis indicated that cattle population, geographical location, dog presence, season, global farm hygiene or the presence of abortion were significantly associated with seroprevalence.

Resistance of Trichostrongylus spp. (Nematoda) to benzimidazole in Algerian cattle herds grazed with sheep

Drug resistance in cattle strongyles to anthelmintics is rarely described in Europe and in the Mediterranean areas. The faecal egg counts are usually low in cattle, and detection of resistance may be particularly difficult. We used the method of twice-repeated treatments and used various estimations of efficacies in order to detect cattle strongyle resistance to benzimidazoles. Resistance was found in two farms among eight that were studied. Trichostrongylus axei was the resistant species in one farm whereas Trichostrongylus sp. was found in another farm. In both farms, there were sheep flocks with a history of strongyle benzimidazole resistance and practice of alternate or mixed grazing with cattle.

Neospora caninum Is Associated With Abortion In Algerian Cattle

Neospora caninum is a major cause of abortion in cattle worldwide. However, little information is available for Algeria. Accordingly, 799 cattle from 87 farms in the north and northeast of Algeria were enrolled in a seroepidemiological survey. An indirect fluorescence antibody test (IFAT) revealed a seroprevalence of 19.6%. The animals were divided into 3 groups according to their breed: imported European cattle, local breeds, and crossed animals (European × local). Seroprevalences were 16.0%, 34.3%, and 18.6% in groups 1, 2, and 3, respectively. A case control study was performed to investigate the link between global seropositivity to N. caninum and abortion risk in those cattle farms. There was a significant (P < 0.01) association between the seroprevalence against N. caninum and the occurrence of abortion in those farms (odds ratio [OR] = 12.03). This was also observed at the individual level (OR = 2.79). The analysis of results according to the breed revealed a significant association between seroprevalence and abortion in groups 1 and 3, but not for group 2, despite the fact that the highest seroprevalence was observed in group 2. Cerebral tissues from 5 aborted fetuses were available for histology and polymerase chain reaction (PCR). One sample was found positive both by histology and by PCR, 2 samples were positive by PCR only, and 2 samples were negative in both tests.

Serological and virological studies on the infectious bovine rhinotracheitis in Algeria.

Clinical cases of infectious bovine rhinotracheitis and infectious pustular vulvovaginitis were observed within the cattle population in Algeria. The isolation and identification of six bovine herpes virus type 1 strains from diseased cattle and the serological surveys undertaken in the central region of the country on 2948 sera revealed a 20.5% incidence of IBR infections in Algeria. The DNA restriction profiles of the isolated strains showed the existence of only the BHV1-2a subtype associated with the endemic infections in Algerian cattle. These results emphasized also the lack of correlation between the observed clinical symptoms and the DNA restriction pattern of the isolated herpes virus strain. The plans for disease control are based mainly on the application of strict control measures at the borders for avoiding the introduction of other BHV1 subtypes.