We use results from the theory of algebras with polynomial identities (PI-algebras) to study the witness complexity of matrix identities. A matrix identity of [Formula: see text] matrices over a field [Formula: see text]is a non-commutative polynomial (f(x1, …, xn)) over [Formula: see text], such that [Formula: see text] vanishes on every [Formula: see text] matrix assignment to its variables. For every field [Formula: see text]of characteristic 0, every [Formula: see text] and every finite basis of [Formula: see text] matrix identities over [Formula: see text], we show there exists a family of matrix identities [Formula: see text], such that each [Formula: see text] has [Formula: see text] variables and requires at least [Formula: see text] many generators to generate, where the generators are substitution instances of elements from the basis. The lower bound argument uses fundamental results from PI-algebras together with a generalization of the arguments in [P. Hrubeš, How much commutativity is needed to prove polynomial identities? Electronic colloquium on computational complexity, ECCC, Report No.: TR11-088, June 2011].We apply this result in algebraic proof complexity, focusing on proof systems for polynomial identities (PI proofs) which operate with algebraic circuits and whose axioms are the polynomial-ring axioms [P. Hrubeš and I. Tzameret, The proof complexity of polynomial identities, in Proc. 24th Annual IEEE Conf. Computational Complexity, CCC 2009, 15–18 July 2009, Paris, France (2009), pp. 41–51; Short proofs for the determinant identities, SIAM J. Comput. 44(2) (2015) 340–383], and their subsystems. We identify a decrease in strength hierarchy of subsystems of PI proofs, in which the [Formula: see text]th level is a sound and complete proof system for proving [Formula: see text] matrix identities (over a given field). For each level [Formula: see text] in the hierarchy, we establish an [Formula: see text] lower bound on the number of proof-steps needed to prove certain identities.Finally, we present several concrete open problems about non-commutative algebraic circuits and speed-ups in proof complexity, whose solution would establish stronger size lower bounds on PI proofs of matrix identities, and beyond.