Airship Research Articles

The Establishment of Economic Dirigism In Maritime Trade of Turkey, 1923–1950

ABSTRACT In the aftermath of the establishment of the republican regime in Turkey in 1923, the policy of economic dirigisme began to dominate the markets step by step. This article aims to analyse the effects of economic dirigisme on maritime trade for a quarter of a century and furnishes an illustrative case delineating how the government’s favoured economic policy attenuated the developmental prospects of maritime commerce. Throughout this temporal span, endeavours advocating a laissez-faire market policy found themselves systematically marginalized, as the juggernaut of economic dirigisme unfolded. The corollary of this policy was a precipitous decline in the operational efficiency of maritime trade. The proclivity for deploying antiquated ships escalated the inherent risks of maritime accidents. Legislative initiatives, exemplified by the promulgation of cabotage laws and the imposition of onerous taxes, erected formidable impediments that curtailed the operability of foreign-flagged vessels in Turkish ports. Concurrently, the waning trade potential cast a pall over the developmental trajectory of Anatolian cities.

Ectopic and transient expression of VvDIR4 gene in Arabidopsis and grapes enhances resistance to anthracnose via affecting hormone signaling pathways and lignin production.

DIR (Dirigent) proteins play important roles in the biosynthesis of lignin and lignans and are involved in various processes such as plant growth, development, and stress responses. However, there is less information about VvDIR proteins in grapevine (Vitis vinifera L). In this study, we used bioinformatics methods to identify members of the DIR gene family in grapevine and identified 18 VvDIR genes in grapevine. These genes were classified into 5 subfamilies based on phylogenetic analysis. In promoter analysis, various plant hormones, stress, and light-responsive cis-elements were detected. Expression profiling of all genes following Colletotrichum gloeosporioides infection and phytohormones (salicylic acid (SA) and jasmonic acid (JA)) application suggested significant upregulation of 17 and 6 VvDIR genes, respectively. Further, we overexpressed the VvDIR4 gene in Arabidopsis thaliana and grapes for functional analysis. Ectopic expression of VvDIR4 in A. thaliana and transient expression in grapes increased resistance against C. gloeosporioides and C. higginsianum, respectively. Phenotypic observations showed small disease lesions in transgenic plants. Further, the expression patterns of genes having presumed roles in SA and JA signaling pathways were also influenced. Lignin contents were measured before and after C. higginsianum infection; the transgenic A. thaliana lines showed higher lignin content than wild-type, and a significant increase was observed after C. higginsianum infection. Based on the findings, we surmise that VvDIR4 is involved in hormonal and lignin synthesis pathways which regulate resistance against anthracnose. Our study provides novel insights into the function of VvDIR genes and new candidate genes for grapevine disease resistance breeding programs.

Genome-Wide Identification, Evolution, and Expression Analysis of the Dirigent Gene Family in Cassava (Manihot esculenta Crantz)

Dirigent (DIR) genes play a pivotal role in plant development and stress adaptation. Manihot esculenta Crantz, commonly known as cassava, is a drought-resistant plant thriving in tropical and subtropical areas. It is extensively utilized for starch production, bioethanol, and animal feed. However, a comprehensive analysis of the DIR family genes remains unexplored in cassava, a crucial cash and forage crop in tropical and subtropical regions. In this study, we characterize a total of 26 cassava DIRs (MeDIRs) within the cassava genome, revealing their uneven distribution across 13 of the 18 chromosomes. Phylogenetic analysis classified these genes into four subfamilies: DIR-a, DIR-b/d, DIR-c, and DIR-e. Comparative synteny analysis with cassava and seven other plant species (Arabidopsis (Arabidopsis thaliana), poplar (Populus trichocarpa), soybean (Glycine max), tomato (Solanum lycopersicum), rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum)) provided insights into their likely evolution. We also predict protein interaction networks and identify cis-acting elements, elucidating the functional differences in MeDIR genes. Notably, MeDIR genes exhibited specific expression patterns across different tissues and in response to various abiotic and biotic stressors, such as pathogenic bacteria, cadmium chloride (CdCl2), and atrazine. Further validation through quantitative real-time PCR (qRT-PCR) confirmed the response of DIR genes to osmotic and salt stress. These findings offer a comprehensive resource for understanding the characteristics and biological functions of MeDIR genes in cassava, enhancing our knowledge of plant stress adaptation mechanisms.

Functional and evolutionary comparative analysis of the DIR gene family in Nicotiana tabacum L. and Solanum tuberosum L.

BackgroundThe dirigent (DIR) genes encode proteins that act as crucial regulators of plant lignin biosynthesis. In Solanaceae species, members of the DIR gene family are intricately related to plant growth and development, playing a key role in responding to various biotic and abiotic stresses. It will be of great application significance to analyze the DIR gene family and expression profile under various pathogen stresses in Solanaceae species.ResultsA total of 57 tobacco NtDIRs and 33 potato StDIRs were identified based on their respective genome sequences. Phylogenetic analysis of DIR genes in tobacco, potato, eggplant and Arabidopsis thaliana revealed three distinct subgroups (DIR-a, DIR-b/d and DIR-e). Gene structure and conserved motif analysis showed that a high degree of conservation in both exon/intron organization and protein motifs among tobacco and potato DIR genes, especially within members of the same subfamily. Total 8 pairs of tandem duplication genes (3 pairs in tobacco, 5 pairs in potato) and 13 pairs of segmental duplication genes (6 pairs in tobacco, 7 pairs in potato) were identified based on the analysis of gene duplication events. Cis-regulatory elements of the DIR promoters participated in hormone response, stress responses, circadian control, endosperm expression, and meristem expression. Transcriptomic data analysis under biotic stress revealed diverse response patterns among DIR gene family members to pathogens, indicating their functional divergence. After 96 h post-inoculation with Ralstonia solanacearum L. (Ras), tobacco seedlings exhibited typical symptoms of tobacco bacterial wilt. The qRT-PCR analysis of 11 selected NtDIR genes displayed differential expression pattern in response to the bacterial pathogen Ras infection. Using line 392278 of potato as material, typical symptoms of potato late blight manifested on the seedling leaves under Phytophthora infestans infection. The qRT-PCR analysis of 5 selected StDIR genes showed up-regulation in response to pathogen infection. Notably, three clustered genes (NtDIR2, NtDIR4, StDIR3) exhibited a robust response to pathogen infection, highlighting their essential roles in disease resistance.ConclusionThe genome-wide identification, evolutionary analysis, and expression profiling of DIR genes in response to various pathogen infection in tobacco and potato have provided valuable insights into the roles of these genes under various stress conditions. Our results could provide a basis for further functional analysis of the DIR gene family under pathogen infection conditions.

Four sugarcane ScDIR genes contribute to lignin biosynthesis and disease resistance to Sporisorium scitamineum

Sugarcane (Saccharum spp.) is a major sucrose and bioenergy crop in the world. The fungal pathogen Sporisorium scitamineum causes sugarcane smut, a devastating disease that destroys stalks and reduces sugar content in sugarcane. This disease can be controlled most effectively by applying smut-resistant sugarcane varieties. Previous studies have shown that Dirigent (DIR) genes are involved in the synthesis of the lignin precursor pinoresinol, which plays a crucial role in plant resistance to biotic stresses. However, the immune response of the DIR homologs in sugarcane (ScDIR) has not been reported yet. In this study, we found that the lignin content of smut-resistant sugarcane varieties (ZZ1, ZZ6, and ZZ9) was significantly higher than that of smut-susceptible varieties (GT42, ROC22, and FN41), and the lignin content of sugarcane increased after smut infection. The smut-resistant and smut-susceptible clones derived from the same genetic population (ROC25 × YZ89-7) showed similar patterns. Quantitative real-time PCR assays revealed that among the 64 DIR genes in sugarcane, ScDIR5, ScDIR7, ScDIR11, and ScDIR40 showed elevated expression after S. scitamineum infection. In vitro coupling reactions showed that the four corresponding ScDIR proteins could mediate the coupling of coniferyl alcohol and its conversion into the lignin precursor pinoresinol. Overexpression of the four ScDIR genes in Nicotiana benthamiana enhanced disease resistance to the fungal pathogens Sclerotium rolfsii, Rhizoctonia solani, and Botrytis cinerea. Moreover, transgenic sugarcane overexpressing these ScDIR genes showed enhanced resistance to smut disease. Taken together, our findings provide evidence that sugarcane ScDIR genes can improve the resistance of plants to fungal pathogens and highlight their potentials in sugarcane breeding for disease resistance.

Genome-wide identification and expression profiling analysis of DIR gene family in Setaria italica.

Dirigent (DIR) proteins play essential roles in regulating plant growth and development, as well as enhancing resistance to abiotic and biotic stresses. However, the whole-genome identification and expression profiling analysis of DIR gene family in millet (Setaria italica (Si)) have not been systematically understood. In this study, we conducted genome-wide identification and expression analysis of the S. italica DIR gene family, including gene structures, conserved domains, evolutionary relationship, chromosomal locations, cis-elements, duplication events, gene collinearity and expression patterns. A total of 38 SiDIR members distributed on nine chromosomes were screened and identified. SiDIR family members in the same group showed higher sequence similarity. The phylogenetic tree divided the SiDIR proteins into six subfamilies: DIR-a, DIR-b/d, DIR-c, DIR-e, DIR-f, and DIR-g. According to the tertiary structure prediction, DIR proteins (like SiDIR7/8/9) themselves may form a trimer to exert function. The result of the syntenic analysis showed that tandem duplication may play the major driving force during the evolution of SiDIRs. RNA-seq data displayed higher expression of 16 SiDIR genes in root tissues, and this implied their potential functions during root development. The results of quantitative real-time PCR (RT-qPCR) assays revealed that SiDIR genes could respond to the stress of CaCl2, CdCl, NaCl, and PEG6000. This research shed light on the functions of SiDIRs in responding to abiotic stress and demonstrated their modulational potential during root development. In addition, the membrane localization of SiDIR7/19/22 was confirmed to be consistent with the forecast. The results above will provide a foundation for further and deeper investigation of DIRs.

Genome-wide identification, characterization, evolution and expression analysis of the DIR gene family in potato (Solanum tuberosum).

The dirigent (DIR) gene is a key player in environmental stress response and has been identified in many multidimensional tube plant species. However, there are few studies on the StDIR gene in potato. In this study, we used genome-wide identification to identify 31 StDIR genes in potato. Among the 12 potato chromosomes, the StDIR gene was distributed on 11 chromosomes, among which the third chromosome did not have a family member, while the tenth chromosome had the most members with 11 members. 22 of the 31 StDIRs had a classical DIR gene structure, with one exon and no intron. The conserved DIR domain accounts for most of the proteins in the 27 StDIRs. The structure of the StDIR gene was analyzed and ten different motifs were detected. The StDIR gene was divided into three groups according to its phylogenetic relationship, and 22 duplicate genes were identified. In addition, four kinds of cis-acting elements were detected in all 31 StDIR promoter regions, most of which were associated with biotic and abiotic stress. The findings demonstrated that the StDIR gene exhibited specific responses to cold stress, salt stress, ABA, and drought stress. This study provides new candidate genes for improving potato's resistance to stress.

WRKY11 up-regulated dirigent expression to enhance lignin/lignans accumulation in Lilium regale Wilson during response to Fusarium wilt

Lilium are highly economically valuable ornamental plants that are susceptible to Fusarium wilt caused by Fusarium oxysporum. Lilium regale Wilson, a wild lily native to China, is highly resistant to F. oxysporum. In this study, a WRKY transcription factor, WRKY11, was isolated from L. regale, and its function during the interaction between L. regale and F. oxysporum was characterized. The ectopic expression of LrWRKY11 in tobacco increased the resistance to F. oxysporum, moreover, the transcriptome sequencing and UHPLC-MS/MS analysis indicated that the methyl salicylate and methyl jasmonate levels rose in LrWRKY11 transgenic tobacco, meanwhile, the expression of lignin/lignans biosynthesis-related genes including a dirigent (DIR) was up-regulated. The lignin/lignans contents in LrWRKY11-transgenic tobacco also significantly increased compared with the wild-type tobacco. In addition, the resistance of L. regale scales in which LrWRKY11 expression was silenced by RNAi evidently decreased, and additionally, the expression of lignin/lignans biosynthesis-related genes including LrDIR1 was significantly suppressed. Therefore, LrDIR1 and its promoter (PLrDIR1) sequence containing the W-box element were isolated from L. regale. The interaction assay indicated that LrWRKY11 specifically bound to the W-box element in PLrDIR1 and activated LrDIR1 expression. Additionally, β-glucuronidase activity in the transgenic tobacco co-expressing LrWRKY11/PLrDIR1-β-glucuronidase was higher than that in transgenic tobacco expressing PLrDIR1-β-glucuronidase alone. Furthermore, the ectopic expression of LrDIR1 in tobacco enhanced the resistance to F. oxysporum and increased the lignin/lignans accumulation. In brief, this study revealed that LrWRKY11 positively regulated L. regale resistance to F. oxysporum through interaction with salicylic acid/jasmonic acid signaling pathways and modulating LrDIR1 expression to accumulate lignin/lignans.

Optimization of Shipping Routes for Container Ships from Indonesia to the Asia-Pacific Using Heuristic Algorithms

Sea transportation such as that by container ships has an essential role in the economy both locally and internationally. Ships are a major commodity in distributing goods over long distances due to their relatively low price compared to air shipping. The study implemented an optimization method using heuristic algorithms with ship route selection to minimize operational costs based on the parameters of mileage between 12 ports in the Asia-Pacific region. The ship speed, engine power, and fuel prices at each port are processed using asymmetric traveling salesman problem modeling (ATSP). The research uses three different algorithms to compare with the performance of the traveling salesman problem, namely the nearest neighbor algorithm, simulated annealing, and a genetic algorithm, with an objective function of keeping fuel costs that ships will incur to a minimum. The results show that the genetic algorithm provides the route with the lowest fuel cost.

QTL mapping of shoot and seed traits impacted by Drought in Barley using a recombinant inbred line Population

BackgroundWith ongoing climate change, drought events are severely limiting barley production worldwide and pose a significant risk to the malting, brewing and food industry. The genetic diversity inherent in the barley germplasm offers an important resource to develop stress resiliency. The purpose of this study was to identify novel, stable, and adaptive Quantitative Trait Loci (QTL), and candidate genes associated with drought tolerance. A recombinant inbred line (RIL) population (n = 192) developed from a cross between the drought tolerant ‘Otis’ barley variety, and susceptible ‘Golden Promise’(GP) was subjected to short-term progressive drought during heading in the biotron. This population was also evaluated under irrigated and rainfed conditions in the field for yields and seed protein content.ResultsBarley 50k iSelect SNP Array was used to genotype the RIL population to elucidate drought-adaptive QTL. Twenty-three QTL (eleven for seed weight, eight for shoot dry weight and four for protein content) were identified across several barley chromosomes. QTL analysis identified genomic regions on chromosome 2 and 5 H that appear to be stable across both environments and accounted for nearly 60% variation in shoot weight and 17.6% variation in seed protein content. QTL at approximately 29 Mbp on chromosome 2 H and 488 Mbp on chromosome 5 H are in very close proximity to ascorbate peroxidase (APX) and in the coding sequence of the Dirigent (DIR) gene, respectively. Both APX and DIR are well-known key players in abiotic stress tolerance in several plants. In the quest to identify key recombinants with improved tolerance to drought (like Otis) and good malting profiles (like GP), five drought tolerant RILs were selected for malt quality analysis. The selected drought tolerant RILs exhibited one or more traits that were outside the realms of the suggested limits for acceptable commercial malting quality.ConclusionsThe candidate genes can be used for marker assisted selection and/or genetic manipulation to develop barley cultivars with improved tolerance to drought. RILs with genetic network reshuffling necessary to generate drought tolerance of Otis and favorable malting quality attributes of GP may be realized by screening a larger population.

Genome-Wide Identification and Expression Pattern Analysis of Dirigent Members in the Genus Oryza.

Dirigent (DIR) members have been shown to play essential roles in plant growth, development and adaptation to environmental changes. However, to date, there has been no systematic analysis of the DIR members in the genus Oryza. Here, 420 genes were identified from nine rice species to have the conserved DIR domain. Importantly, the cultivated rice species Oryza sativa has more DIR family members than the wild rice species. DIR proteins in rice could be classified into six subfamilies based on phylogeny analysis. Gene duplication event analysis suggests that whole genome/segmental duplication and tandem duplication are the primary drivers for DIR genes' evolution in Oryza, while tandem duplication is the main mechanism of gene family expansion in the DIR-b/d and DIR-c subfamilies. Analysis of the RNA sequencing data indicates that OsjDIR genes respond to a wide range of environmental factors, and most OsjDIR genes have a high expression level in roots. Qualitative reverse transcription PCR assays confirmed the responsiveness of OsjDIR genes to the undersupply of mineral elements, the excess of heavy metals and the infection of Rhizoctonia solani. Furthermore, there exist extensive interactions between DIR family members. Taken together, our results shed light on and provide a research foundation for the further exploration of DIR genes in rice.

A Review of Cases concerning Sea and Air Shipping and Air area - Focusing on cases around 2020

A Review of Cases concerning Sea and Air Shipping and Air area - Focusing on cases around 2020

Cloning and Expression Analysis of <i>PeDIR19</i> Gene in Poplar

Dirigent ( DIR ) gene plays an important role in the formation of lignin and lignans by controlling the stereoselective coupling of pineperin alcohol monomers in plants during the polymerization of lignin and lignans. In this study, we take the ‘Nanlin 895’ ( Populus deltoides × P. euramericana  cv ‘Nanlin 895’) as the material, and clone the PeDIR19  gene. PeDIR19 , the whole length of 588 bp, has a length of 555 bp in ORF region and has only a length of 33 bp intron in the middle. PeDIR19 protein codes 185 amino acids and is a hydrophobic protein. It has four conservative motifs and has a highly conservative structural functional domain between 50 bp ~ 142 bp. PeDIR19  gene was found to have the highest homology with Populus trichocarpa   PtDIR19 ( Potri.001G009100 ), with only four bases differences. By phylogenetic analysis, PeDIR19  gene belongs to DIR -B/D subfamily. Through tissue specific expression analysis of ‘Nanlin 895’ with different growth stages, it was found that PeDIR19  gene expression level was the highest in the root during the tissue culture seedlings and in the stem during the pot seedlings. PeDIR19  gene had a strong response after 1 d under the induction of Yangpan Erbaojun ( Marssonina brunnea ). Under the induction of salicylic acid, methyl jasmonate and abscisic acid stress, PeDIR19  gene can strongly respond to the stress of methyl jasmonate and abscisic acid and weakly respond to the salicylic acid stress. This study provides a solid foundation for further study on the function identification of DIR  gene in the biosynthetic pathway of plant resistance.

Unintended Consequences of Environmental Regulation of Maritime Shipping: Carbon Leakage to Air Shipping

Unintended Consequences of Environmental Regulation of Maritime Shipping: Carbon Leakage to Air Shipping

Pathogen Resistance Depending on Jacalin-Dirigent Chimeric Proteins Is Common among Poaceae but Absent in the Dicot Arabidopsis as Evidenced by Analysis of Homologous Single-Domain Proteins.

MonocotJRLs are Poaceae-specific two-domain proteins that consist of a jacalin-related lectin (JRL) and a dirigent (DIR) domain which participate in multiple developmental processes, including disease resistance. For OsJAC1, a monocotJRL from rice, it has been confirmed that constitutive expression in transgenic rice or barley plants facilitates broad-spectrum disease resistance. In this process, both domains of OsJAC1 act cooperatively, as evidenced from experiments with artificially separated JRL- or DIR-domain-containing proteins. Interestingly, these chimeric proteins did not evolve in dicotyledonous plants. Instead, proteins with a single JRL domain, multiple JRL domains or JRL domains fused to domains other than DIR domains are present. In this study, we wanted to test if the cooperative function of JRL and DIR proteins leading to pathogen resistance was conserved in the dicotyledonous plant Arabidopsis thaliana. In Arabidopsis, we identified 50 JRL and 24 DIR proteins, respectively, from which seven single-domain JRL and two single-domain DIR candidates were selected. A single-cell transient gene expression assay in barley revealed that specific combinations of the Arabidopsis JRL and DIR candidates reduced the penetration success of barley powdery mildew. Strikingly, one of these pairs, AtJAX1 and AtDIR19, is encoded by genes located next to each other on chromosome one. However, when using natural variation and analyzing Arabidopsis ecotypes that express full-length or truncated versions of AtJAX1, the presence/absence of the full-length AtJAX1 protein could not be correlated with resistance to the powdery mildew fungus Golovinomyces orontii. Furthermore, an analysis of the additional JRL and DIR candidates in a bi-fluorescence complementation assay in Nicotiana benthamiana revealed no direct interaction of these JRL/DIR pairs. Since transgenic Arabidopsis plants expressing OsJAC1-GFP also did not show increased resistance to G. orontii, it was concluded that the resistance mediated by the synergistic activities of DIR and JRL proteins is specific for members of the Poaceae, at least regarding the resistance against powdery mildew. Arabidopsis lacks the essential components of the DIR-JRL-dependent resistance pathway.

Air pollutant emission calculation and shipping costs: The case of yacht transport between the North Sea and Mediterranean ports

This research aims to determine the optimal transport mode for yacht relocation on a selected shipping route by calculating and comparing air emissions of yacht transport as deck cargo on a cargo ship with those from conventional navigation. Based on the most recent findings, the main emission pollutant categories of CO2, SOx, NOx, PM10, and PM2.5 were estimated and compared between the two options. The cost-effectiveness criterion of selected transport modes was an auxiliary objective of this study. With the created mathematical modeling method application, considering the individual vessel specifications, performance, and route characteristics, the results evidenced higher external costs of a cargo ship transporting yachts onboard versus conventional yacht voyage on examined traffic route by 7.7 times. On the contrary, the economic implications showed higher yacht voyage costs by almost two times. With the possibility of exploiting the principle of economies of scale for a general cargo ship, the yacht transport on deck has the comparative advantage considering the total sum of external costs generated by the transport modes in this study. These results might have implications for creating policies on emission control and sustainable development of the shipping industry. They encourage a more restrictive policy considering the environmental transport activities impacts, including the requirement of the complete application of external cost internalization and the more rigorous and precise implementation of the "polluter pays" principle.

Electric Vehicle Routing Problem with Simultaneous Pickup and Delivery: Mathematical Modeling and Adaptive Large Neighborhood Search Heuristic Method

Electric vehicles (EVs) are a promising option to reduce air pollution and shipping costs, especially in urban areas. To provide scientific guidance for the growing number of logistics companies using EVs, we investigated an electric-vehicle-routing problem with simultaneous pickup and delivery that also considers non-linear charging and load-dependent discharging (EVRPSPD-NL-LD). The objective was to minimize the total number of EVs and the total working time, including travel time, charging time, waiting time, and service time. We formulated the problem as a mixed integer linear program (MILP), and small-size problems could be solved to optimality in an acceptable amount of time using the commercial solver IBM ILOG CPLEX Optimization Studio (CPLEX). In view of the fact that the problem is NP-hard, an adaptive large neighborhood search (ALNS) metaheuristic method was proposed to solve large-size problems. Meanwhile, new operators and a time priority approach were developed to provide options for different scenarios. The results of our computational investigation and sensitivity analysis showed that the proposed methods are effective and efficient for modified benchmark instances.

Assembly and Testing of a Hydrogen Fuel Cell System to Power an Airship

An airship, or dirigible balloon, is an aircraft that gains its lift from a gas less dense than air, like helium. For traction, airships have two or more propellers normally attached to a gondola that is suspended under the balloon. At present, they are used for hovering long times without the need for a high speed, like for aerial observation, advertising, or as camera platforms. In our laboratory, the airship is being used as a platform for testing hydrogen fuel cell systems for aerial applications.The airship used in this communication is a 3 m3 polyurethane's inflatable zeppelin, with radio control, and three electrical propellers (Fig. 1a). A hydrogen fuel cell system has been developed and integrated with the gondola for powering the propellers and electronics. The fuel cell is a 15 W PEMFC stack prototype able to work fully under passive feeding conditions (Fig. 1b). In addition, three supercapacitors have been implemented, that are recharged by the fuel cell, for eventual larger power demands up to 40 W. Hydrogen is stored in two metal hydride canisters with 2g capacity to provide 30 W h energy enough for a few hours autonomy. In addition, for fuel cell parameters monitoring, different sensors have been implemented that provide voltages of the individual cells, current, and temperatures. The sensors are connected to Arduino board, and measurements are continuously monitored by a Labview software with wifi. Boost dc-dc conversion provides 7V voltage power to feed the motors.The stack prototype allows for different testing configurations, using cells in series and parallel connection to change power characteristics with different voltage and current. Results of the testing are provided in this communication.Acknowledgement: The work is partially financed by the ELHYPORT project (PID2019−110896RB-I00), Spanish Ministry of Science and Innovation. Figure 1

The Crystal Structure of the Defense Conferring Rice Protein OsJAC1 Reveals a Carbohydrate Binding Site on the Dirigent-like Domain.

Pesticides are routinely used to prevent severe losses in agriculture. This practice is under debate because of its potential negative environmental impact and selection of resistances in pathogens. Therefore, the development of disease resistant plants is mandatory. It was shown that the rice (Oryza sativa) protein OsJAC1 enhances resistance against different bacterial and fungal plant pathogens in rice, barley, and wheat. Recently we reported possible carbohydrate interaction partners for both domains of OsJAC1 (a jacalin-related lectin (JRL) and a dirigent (DIR) domain), however, a mechanistic understanding of its function is still lacking. Here, we report crystal structures for both individual domains and the complex of galactobiose with the DIR domain, which revealed a new carbohydrate binding motif for DIR proteins. Docking studies of the two domains led to a model of the full-length protein. Our findings offer insights into structure and binding properties of OsJAC1 and its possible function in pathogen resistance.

Identification of key enzyme genes involved in biosynthesis pathways of lignan and lignin in Eucommia ulmoides based on transcriptome assembly

Lignan is the main medicinal component of Eucommia ulmoides, and lignin is involved in the defense of plants against diseases and insect pests.They are synthesized from coniferyl alcohol with the help of dirigent(DIR) and peroxidase(POD), respectively.In this study, transcriptome assembly of stems and leaves of E.ulmoides was performed, yielding 112 578 unigenes.Among them, 70 459 were annotated in seven databases.A total of 59 unigenes encodes 11 key enzymes in the biosynthesis pathways of lignin and lignin, of which 11 encode POD and 8 encode DIR.A total of 13 unigenes encoding transcription factors are involved in phenylpropanoid metabolism. Compared with leaves of E.ulmoides, 7 575 unigenes were more highly expressed in stems, of which 462 were involved in phenylpropanoid biosynthesis.Our results extend the public transcriptome dataset of E.ulmoides, which provide valuable information for the analysis of biosynthesis pathways of lignan and lignin in E.ulmoides and lay a foundation for further study on the functions and regulation mechanism of key enzymes in lignan and lignin biosynthesis pathways.