Airborne PM2 Research Articles

Field calibration and performance evaluation of low-cost sensors for monitoring airborne PM in the occupational mining environment

Field calibration and performance evaluation of low-cost sensors for monitoring airborne PM in the occupational mining environment

Melatonin protects against particulate matter-induced ovarian dysfunction by activating the Nrf2 signaling pathway to alleviate ferroptosis

Accumulating evidence suggests that exposure to ambient airborne PM2.5 increases the risk of primary ovarian insufficiency (POI). However, whether ferroptosis, a newly discovered type of cell death involved in PM2.5-induced lung injury and fibrosis, is involved in PM2.5-induced POI has not been determined. This study aimed to verify the involvement of PM2.5-induced ferroptosis in ovarian dysfunction and further demonstrate that melatonin inhibits ferroptosis by activating the Nrf2 signaling pathway to ameliorate POI in vivo and in vitro. In our study, PM2.5 promoted iron accumulation and induced lipid peroxidation, thus contributing to ferroptosis in KGN cells and ovaries. However, these effects were eliminated and enhanced in Nrf2-overexpressing and Nrf2-knockdown cells, respectively. In addition, melatonin and ferrostatin-1 (Fer-1) inhibited ferroptosis by activating the NRF2 signaling pathway, as evidenced by the silencing of Nrf2 in vivo and in vitro. Mechanistically, Nrf2-knockout mice were more susceptible to ferroptosis and PM2.5-induced POI than control mice. Moreover, melatonin suppressed changes in morphological and biochemical indicators related to ferroptosis, such as MDA and GSH depletion and GPX4 and XCT downregulation, by enhancing Nrf2 signaling. Here, we first reported that PM2.5 triggered ferroptosis by increasing ROS levels, lipid peroxidation and glutathione depletion. Notably, melatonin significantly decreased ferroptosis levels and improved ovarian function by activating the NRF2 signaling pathway in vivo and in vitro.

Unraveling Potential Causative Components for the Deleterious Effect of Atmospheric Fine Particulate Matter on Red Blood Cells.

Atmospheric fine particulate matter (PM2.5) poses threats to the cardiovascular system. Red blood cells (RBCs) are the most abundant cells in blood, which are actively involved in multiple hematological diseases, such as blood clot formation and thrombosis. Exploring how PM2.5 with spatiotemporal heterogeneity influences the hematological system by targeting RBCs would help gain insights into the deleterious effects of PM2.5 and provide clues for finding the causative components therein. Herein, the PM2.5 samples collected from 3 urban sites in Beijing (i.e., Chaoyang, Shunyi, and Yanqing districts) during 4 seasons of 2022 were studied for their toxicities to mouse RBCs, and the main contributing components were further explored through chemical analysis and correlation measure. The results showed that exposure to PM2.5 samples decreased adenosine triphosphate (ATP) levels and increased phosphatidylserine (PS) externalization of RBCs, causing cell morphological deformity. The Pearson correlation analysis showed that the aromaticity of the dissolved organic matter (DOM) in PM2.5 samples was positively correlated with PS exposure of RBCs, showing that the lignin-like compounds were the potential contributors. The negative correlation of zeta potentials of PM2.5 samples with PS exposure of RBCs showed the particle-derived bioactivities of this airborne pollutant. The simulative test based on artificial nanomaterials of carbon black (CB) and oxidized CB (OCB) confirmed the crucial role of particulate carbon in PM2.5-induced effects on RBCs, and soot with a certain oxidation degree was, thus, recognized as another contributor, given its ubiquitous existence in PM2.5 samples. This study, for the first time, revealed PM2.5-induced PS exposure of RBCs, and the causative components of DOM and soot were unraveled. Considering the inevitable contact of airborne PM2.5 with RBCs in the blood circulatory system, the findings obtained herein would help bridge the gap between PM2.5 exposure and the risk of cardiovascular diseases, like thrombogenesis.

Seasonal dynamics of the phage-bacterium linkage and associated antibiotic resistome in airborne PM2.5 of urban areas

Inhalable microorganisms in airborne fine particulate matter (PM2.5), including bacteria and phage, are major carriers of antibiotic resistance genes (ARGs) with strong ecological linkages and potential health implications for urban populations. A full-spectrum study on ARG carriers and phage-bacterium linkages will shed light on the environmental processes of antibiotic resistance from airborne dissemination to the human lung microbiome. Our metagenomic study reveals the seasonal dynamics of phage communities in PM2.5, their impacts on clinically important ARGs, and potential implications for the human respiratory microbiome in selected cities of China. Gene-sharing network comparisons show that air harbours a distinct phage community connected to human- and water-associated viromes, with 57 % of the predicted hosts being potential bacterial pathogens. The ARGs of common antibiotics, e.g., peptide and tetracycline, dominate both the antibiotic resistome associated with bacteria and phages in PM2.5. Over 60 % of the predicted hosts of vARG-carrying phages are potential bacterial pathogens, and about 67 % of these hosts have not been discovered as direct carriers of the same ARGs. The profiles of ARG-carrying phages are distinct among urban sites, but show a significant enrichment in abundance, diversity, temperate lifestyle, and matches of CRISPR (short for ‘clustered regularly interspaced short palindromic repeats’) to identified bacterial genomes in winter and spring. Moreover, phages putatively carry 52 % of the total mobile genetic element (MGE)-ARG pairs with a unique ‘flu season’ pattern in urban areas. This study highlights the role that phages play in the airborne dissemination of ARGs and their delivery of ARGs to specific opportunistic pathogens in human lungs, independent of other pathways of horizontal gene transfer. Natural and anthropogenic stressors, particularly wind speed, UV index, and level of ozone, potentially explained over 80 % of the seasonal dynamics of phage-bacterial pathogen linkages on antibiotic resistance. Therefore, understanding the phage-host linkages in airborne PM2.5, the full-spectrum of antibiotic resistomes, and the potential human pathogens involved, will be of benefit to protect human health in urban areas.

Skyrocketing pollution: assessing the environmental fate of July 4th fireworks in New York City.

Pyrotechnic displays often lead to significant increases in poor air quality. The widespread environmental fate-involving air, water, and spatial-temporal analyses-of fireworks-produced pollutants has seldom been investigated. This study examined the environmental fate of pollutants from the largest fireworks event in the U.S.: Macy's Fourth of July Fireworks show in New York City (NYC). Real-time PM2.5 and gravimetric PM2.5 and PM10 were collected at locations along the East River of NYC. Airborne particles were assayed for trace elements (X-ray fluorescence) and organic and elemental carbon (OC/EC). River water samples were evaluated by ICP-MS for heavy-metal water contamination. Spatial-temporal analyses were created using PM2.5 concentrations reported by both EPA and PurpleAir monitoring networks for NYC and 5 other major metropolitan areas. The fireworks event resulted in large increases in PM2.5 mass concentrations at the river-adjacent sampling locations. While background control PM2.5 was 10-15 µg/m3, peak real-time PM2.5 levels exceeded 3000 µg/m3 at one site and 1000 µg/m3 at two other locations. The integrated gravimetric PM2.5 and PM10 concentrations during the fireworks event ranged from 162 to 240 µg/m3 and 252 to 589 µg/m3, respectively. Zn, Pb, Sb, and Cu more than doubled in river water samples taken after the event, while S, K, Ba, Cu, Mg, Fe, Sr, Ti, and Zn increased in airborne PM2.5 from the fireworks. Data from hyperlocal monitoring networks for NYC and other metropolitan areas yielded similar, but generally smaller, increases in PM2.5 levels. Fireworks shows have been associated with environmental contamination. This comprehensive analysis considers the fate of pollutants from the largest annual U.S. pyrotechnic show through air, water, and hyperlocal temporal characterization.

The impacts of reduction in ambient fine particulate air pollution on natural-cause mortality in Taiwan

ABSTRACT Many epidemiologic studies have reported an association between high concentrations of fine particulate matter (PM2.5) and increased mortality rates. Concurrently an association between decreased concentration of these airborne PM2.5 pollutants and a decline in mortality frequency was noted in certain investigations globally; however, only a very few of these studies were conducted in Asia. Taiwan was found to exhibit a 30% decline in ambient PM2.5 levels over the last 20 years. The aim of this ecological investigation was to examine the contribution of annual reductions in ambient PM2.5 to changes in age-standardized natural-cause mortality rates (ASRs) in 65 townships in Taiwan from 2006 to 2020 controlling for lung cancer mortality rate, physician density, and annual household income. Data demonstrated a 0.9/105 fall in adjusted ASR for every 10 ug/m3 reduction in mean annual PM2.5 level in Taiwan during this 14-year period, suggesting a significant association between reductions in ambient PM2.5 levels and decreases in natural-cause mortality rates.

Insights into the variations of polycyclic aromatic hydrocarbons and their nitrated and oxygenated derivatives in urban airborne PM2.5

Polycyclic aromatic hydrocarbons (PAHs) can be transformed into more toxic species in the atmosphere, but field evidence for their transformation remains rare. Here, polycyclic aromatic compounds (PACs), including PAHs and their nitrated and oxygenated derivatives (NPAHs and OPAHs), in fine particulate matter (PM2.5) were measured in the seasons at multiple locations in a megacity (Guangzhou, China). Molecular diagnostic ratios (MDRs), meteorological factors, and atmospheric oxidant measurements were employed to understand the atmospheric behaviors of PM2.5-bound PACs. The results indicated that similarities/differences in the short-term variations of PAC concentrations between the locations depended on their sources and seasons. The temporal trends in MDRs were more distinct than those in concentrations among the locations. Meteorological factors affected PAC concentrations through a similar pathway (demodulating loadings on PM). Their influence on the ratios can be explained by compounds’ atmospheric half-life, vapor pressure, or particle-size distribution. Temperature was the most powerful factor for both the concentrations and MDRs. Relative humidity was minor, but its positive influence on the reaction to OH radicals was appreciable. The dependence on PM2.5 concentrations suggests that PM plays a more significant role in the sorption or formation of N/OPAHs than in their parent compounds. The OH radical-initiated reaction is the major formation mechanism of N/OPAHs throughout the year, but substantial reactions to NO3 radical in autumn were observed due to high ozone levels. Multiple linear regression revealed that parent PAHs, NO2, and/or O3 are significant contributing factors to the secondary formation of N/OPAHs. Partial least squares-discriminant analysis showed the distinguishing characteristics of summer and autumn PM2.5-bound PACs. This study provides field evidence for the gaseous and heterogeneous reactions of PAHs.

Nature-based solutions for monitoring the impact of vehicular particulate matter and for the preventive conservation of the Palatine Hill archaeological site in Rome, Italy

Magnetic and chemical biomonitoring methodologies were applied to the southern slopes of the Palatine Hill archaeological area in Rome, Italy. Plant leaves and lichen transplants were respectively sampled and exposed between July 2022 and June 2023 to assess the impact of vehicular particulate matter from Via dei Cerchi, a trafficked road coasting Circus Maximus, towards the archaeological area upon the Palatine Hill. The magnetic properties of leaves and lichens, inferred from magnetic susceptibility, hysteresis loops and first order reversal curves, were combined with the concentration of trace elements. It was demonstrated that the bioaccumulation of magnetite-like particles, associated with tracers of vehicular emissions, such as Ba and Sb, decreased with longitudinal distance from the road, without any important influence of elevation from the ground. Lichens demonstrated to be more efficient biomonitors of airborne PM than leaves, irrespective of the plant species. Conversely, leaves intercepted and accumulated all PM fractions, including road dusts and resuspended soil particles. Thus, plant leaves are suitable for providing preventive conservation services that limit the impact of particulate pollution on cultural heritage sites within busy metropolitan contexts.

PM2.5 and PM10 Airborne Concentrations Resulting from Fireworks During Festivities: A Systematic Review

PM2.5 and PM10 Airborne Concentrations Resulting from Fireworks During Festivities: A Systematic Review

The use of honey bees (Apis mellifera L.) to monitor airborne particulate matter and assess health effects on pollinators.

The honey bee Apis mellifera has long been recognized as an ideal bioindicator for environmental pollution. These insects are exposed to pollutants during their foraging activities, making them effective samplers of environmental contaminants, including heavy metals, pesticides, radionuclides, and volatile organic compounds. Recently, it has been demonstrated that honey bees can be a valuable tool for monitoring and studying airborne PM pollution, a complex mixture of particles suspended in the air, known to have detrimental effects on human health. Airborne particles attached to the bees can be characterised for their morphology, size, and chemical composition using a scanning electron microscopy coupled with X-ray spectroscopy, thus providing key information on the emission sources of the particles, their environmental fate, and the potential to elicit inflammatory injury, oxidative damage, and other health effects in living organisms. Here, we present a comprehensive summary of the studies involving the use of honey bees to monitor airborne PM, including the limits of this approach and possible perspectives. The use of honey bees as a model organism for ecotoxicological studies involving pollutant PM is also presented and discussed, further highlighting the role of the bees as a cornerstone of human, animal, and environmental health, according to the principles of the "One Health" approach.

Sampling, composition, and biological effects of Mexico City airborne particulate matter from multiple periods

Air pollution is a worldwide environmental problem with an impact on human health. Particulate matter of ten micrometers or less aerodynamic diameter (PM10) as well as its fine fraction (PM2.5) is related to multiple pulmonary diseases. The impact of air pollution in Mexico City, and importantly, particulate matter has been studied and considered as a risk factor for two decades ago. Previous studies have reported the composition of Mexico City particulate matter, as well as the biological effects induced by this material. However, material collected and used in previous studies is a limited resource, and sampling and particle recovery techniques have been improved. In this study, we describe the methods used in our laboratory for Mexico City airborne particulate matter PM10 and PM2.5 sampling, considering the years 2017, 2018 and 2019. We also analyzed the PM10 and PM2.5 samples obtained to determine their composition. Finally, we exposed lung cell line cultures to PM10 and PM2.5 to evaluate the biological effect of the material in terms of cell viability, cell death, inflammatory response, and cytogenetic alterations. Our results showed that PM10 composition includes inorganic, organic and biological compounds, while PM2.5 is a mixture of more enriched organic compounds. PM10 and PM2.5 treatment in lung cells does not significantly impact cell viability/cell death. However, PM10 and PM2.5 increase the secretion levels of IL-6. Moreover, PM10 as well as PM2.5 induce cytogenetic alterations, such as micronuclei, anaphase bridges, trinucleated cells and apoptotic cells in lung cells. Our results update the evidence of the composition and biological effects of Mexico City particulate matter and provide us a reliable basis for future approaches.

Effects of PM10 Airborne Particles from Different Regions of a Megacity on In Vitro Secretion of Cytokines by a Monocyte Line during Different Seasons.

Several epidemiological studies have demonstrated that particulate matter (PM) in air pollution can be involved in the genesis or aggravation of different cardiovascular, respiratory, perinatal, and cancer diseases. This study assessed the in vitro effects of PM10 on the secretion of cytokines by a human monocytic cell line (THP-1). We compared the chemotactic, pro-inflammatory, and anti-inflammatory cytokines induced by PM10 collected for two years during three different seasons in five different Mexico City locations. MIP-1α, IP-10, MCP-1, TNF-α, and VEGF were the main secretion products after stimulation with 80 μg/mL of PM10 for 24 h. The THP-1 cells showed a differential response to PM10 obtained in the different sites of Mexico City. The PM10 from the north and the central city areas induced a higher pro-inflammatory cytokine response than those from the south. Seasonal pro-inflammatory cytokine secretion always exceeded anti-inflammatory secretion. The rainy-season-derived particles caused the lowest pro-inflammatory effects. We concluded that toxicological assessment of airborne particles provides evidence supporting their potential role in the chronic exacerbation of local or systemic inflammatory responses that may worsen the evolution of some chronic diseases.

Maternal exposure to airborne particulate matter during pregnancy and lactation induces kidney injury in rat dams and their male offspring: the role of vitamin D in pregnancy and beyond.

Little is known about the transgenerational effects of maternal exposure to fine particulate matter (PM2.5) on offspring kidney health. This study investigated the effect of maternal administration of PM2.5 or PM2.5 with vitamin D during pregnancy and lactation on renal injury in rat dams and their offspring. Nine pregnant Sprague-Dawley rats received oral administration of normal saline, airborne PM2.5, or PM2.5 with vitamin D from gestational day 11 to postpartum day 21. Kidneys of rat dams (n = 3 for each group) and their male offspring (n = 5 for each group) were taken for analysis on postpartum or postnatal day 21. Maternal PM2.5 exposure increased glomerular damage, tubulointerstitial injury, and cortical macrophage infiltration in both dams and pups; all increases were attenuated by vitamin D administration. In dam kidneys, PM2.5 increased the protein expression of vitamin D receptor (VDR), klotho, and tumor necrosis factor-α; vitamin D lessened these changes. The expressions of renin, nuclear factor erythroid 2-related factor 2 (Nrf2), and nuclear factor-kappa B (NF-κB) p50 decreased in rat dams exposed to PM2.5. In offspring kidneys, exposure to maternal PM2.5 reduced the expression of VDR, renin, angiotensin-converting enzyme (ACE), Nrf2, and NF-κB p50, but increased cytochrome P450 24A1 expression. Maternal vitamin D administration with PM2.5 enhanced VDR, ACE, and NF-κB p50 activities in pup kidneys. PM2.5 exposure during nephrogenesis may exert transgenerational renal impairment, and maternal vitamin D intake could attenuate PM2.5-induced kidney damage in mothers and their offspring.

Seasonal distribution of human-to-human pathogens in airborne PM2.5 and their potential high-risk ARGs.

Airborne microorganisms, an emerging global health threat, have attracted extensive studies. However, few attentions have been paid to the seasonal distribution of airborne pathogens, in particular their associations with antibiotic resistance genes (ARGs). To this end, two-week daily PM2.5 samples were consecutively collected from Nanchang in four seasons, and the human-to-human pathogens were screened based on high-throughput sequencing. The results showed that there were 20 pathogenic taxa in PM2.5 in Nanchang, and the highest relative abundance of pathogens was observed in winter (5.84%), followed by summer (3.51%), autumn (2.66%), and spring (1.80%). Although more than half of pathogenic taxa were shared by the four seasons, the analysis of similarities showed that pathogenic community was shaped by season (r = 0.16, p < 0.01). Co-occurrence network analysis disclosed significant interactions among pathogens in each season. Moreover, some dominant pathogens such as Plesiomonas shigelloides, Bacteroides fragilis, and Escherichia-Shigella were hub pathogens. In addition, PICRUSt2 predicted that there were 35 high-risk ARG subtypes in PM2.5, and the pathogens had strongly positive correlations with these ARGs. Even some pathogens like Plesiomonas shigelloides, Bacteroides fragilis, Aeromonas, Citrobacter, may be multi-drug resistant pathogens, including beta-lactam, aminoglycosides, chloramphenicol and multi-drug resistances, etc. Both air pollutants and meteorological conditions contributed to the seasonal variation of airborne pathogenic bacteria (r = 0.15, p < 0.01), especially CO, O3, PM2.5, temperature and relative humidity. This study furthers our understanding of airborne pathogens and highlights their associations with ARGs.

Cyclone Ventilator Modification: An Environmentally Friendly Solution for the Homes of Patients With Tuberculosis and Acute Respiratory Infections

Poor indoor air quality is one of the factors that can cause health problems. Tuberculosis (TB) and acute respiratory infections (ARIs) are among the diseases often associated with indoor air quality. In 2022, the highest number of TB cases was recorded in Surabaya, with 4739 cases. The research location, at the Wonokusumo Public Health Center, still reported a high number of TB cases, with 126 cases in 2022 and 84 cases in 2023. The use of Cyclone Ventilator Modification (CVM) has shown to improve the indoor air quality of occupants. This study aims to analyze the capability and the time required for the CVM to enhance the indoor air quality in homes of TB and ARI patients, using indicators such as temperature, humidity, airborne microbiology, air flow rate, PM2.5 dust particles, and lighting. This experimental study uses a One Group Pre‐Test Post‐Test Design to test changes following the experiment. The device operates on the principle of improving air quality, including temperature, humidity, air flow rate, lighting, and the ability to reduce airborne microbiology and PM2.5 dust particles indoors. The effectiveness of the CVM in improving indoor air quality for TB and ARI patients is analyzed using a paired T‐test analysis, revealing significant differences in indoor air quality indicators before and after the device’s operation. The results obtained show that the two‐tailed significance value is less than 0, indicating a significant difference between the initial and final variables. After CVM operation in five homes with eight replications, the CVM’s effectiveness was optimal in relation to room area/volume. This study was conducted as a simple step to minimize pollutants and improve indoor air quality. These findings can be applied and utilized to create healthier buildings or homes.

Dynamometric Investigation on Airborne Particulate Matter from Automobile Brake: Impact of Disc Materials on Brake Emission Factor

In this work, we evaluated the impact of disc rotors of gray cast iron (GCI), nitrocarburized (NC), and superhard ceramic-coated (SCC) GCI on the brake wear PM emissions of passenger vehicles using dynamometric measurements. The brake emission factor (BEF) of the SCC was greatly reduced by more than a factor of 1/5 compared with those for the GCI and NC for both low-steel and non-steel friction materials. Surface topological and microstructural analyses confirmed that more severe wear was pronounced for the NC rotor compared with the SCC, as evidenced by large concave pits in the wear tracks. Analysis of the size-classified airborne PM suggests that reduced micron-sized particles, which originated from the GCI disc, were responsible for the lower BEF due to the increased hardness of the SCC.

Assessment of Daily Exposure to Organophosphate Esters through PM2.5 Inhalation, Dust Ingestion, and Dermal Contact.

Inhalation of airborne fine particulate matter (PM2.5), dust ingestion, and dermal contact with dust are important pathways for human exposure to different contaminants, such as organophosphate esters (OPE), compounds that are widely used as flame retardants and plasticizers. There are limited studies assessing the extent of the contamination of OPE in indoor airborne PM2.5. This study offers a novel approach by examining various indoor environments, such as homes, workplaces, and means of transport, where people typically spend their daily lives. The goal is to provide a comprehensive assessment of daily exposure to these pollutants. Both PM2.5 and dust samples were collected in order to determine the concentration levels of 17 different OPEs. Fifteen OPEs in PM2.5 and 16 in dust samples were detected. Concentration levels in indoor air ranged from 4.37 to 185 ng/m3 (median 24.4 ng/m3) and from 3.02 to 36.9 μg/g for the dust samples (median 10.2 μg/g). Estimated daily intakes (EDIs) of OPEs were calculated for adults, yielding median values of 3.97 ng/(kg bw × day) for EDIInhalation, 5.89 ng/(kg bw × day) for EDIDermal, and 1.75 ng/(kg bw × day) for EDIIngestion. Such levels lie below human health threshold risk limits, although in some cases they could be only 2 times below the threshold for carcinogenic risk, with a main contribution from tris(2-chloroethyl) phosphate (TCEP). Given this threshold proximity, additional exposure to these chemicals from other pathways, such as food ingestion, gas phase exposure, and/or inhalation of coarser particles (PM10-2.5), could therefore lead to health limit exceedances.

Trace elements and microbial community composition associated with airborne PM2.5 in wetlands: A case study in Tablas de Daimiel National Park

Tablas de Daimiel National Park (TDNP) is one of the most important wetlands in the Iberian Peninsula. Due to its location near various cities and new industries focused on agricultural waste revalorization, we investigated concurrently the concentrations of particulate matter 2.5 (PM2.5) mass, trace element composition, and associated microbial communities (bacteria and fungi) during a year-long study. The goal of this study was to explore the dependencies among these physicochemical and microbiological parameters on a seasonal time scale. Additionally, we assessed meteorological conditions and back trajectories to shed light on atmospheric mechanisms and sources related to these elements. We found the variability of PM2.5 to be influenced by local meteorological parameters. Through the analysis of crustal enrichment factors (EFs), bivariate correlations, and air mass patterns, we determined that soil resuspension was the primary contributor to elevated metal concentrations in PM2.5 within the park, followed by other minor sources, such as traffic emissions and Sahara dust intrusions. The measured metal levels were used to calculate the ecological risk in the area, resulting in a low ecological risk index (RI) of 52. Shifts in microbial community structure were observed to be mainly driven by changes in air temperature and Cu concentration. The results from this study contribute to a better understanding of the environmental dynamics in TDNP. Taken together, our findings will aid in the development of effective strategies for its conservation and management.

Variability of airborne microbiome at different urban sites across seasons: a case study in Rome

Introduction: Biogenic fraction of airborne PM10 dominated by bacteria and fungi, has been recognized as serious environmental and human health issues in cities.Methods: In the present study, we combined a high-throughput amplicon sequencing of the bacterial 16S rRNA gene and the fungal internal transcribed spacer (ITS) region, with elemental analysis of airborne particulate matter (PM10) to investigate the community compositions and structures of PM10-associated bacteria and fungi across four different seasons in three urban sites of Rome with differential pollution rate.Results: In this study, а clear seasonal shift of bacterial and fungal community structure driven by PM10 mass concentrations and environmental factors, such as temperature and precipitations, has been identified. In addition, the seasonal impact of local sources and long-range transported air masses on the community structures of the microbes has been also postulated. Our data revealed that the lack of precipitation and the subsequent resuspension of dust produced by vehicular traffic might contribute to the maximum abundance of soil-associated microbes in winter and summer. However, the increase of PM10 concentrations favoured also by climatic conditions, domestic heating and dust advection event from African desert further shaped the community structure of winter. Across three seasons, the pollutant removal-hydrogen oxidation bacteria and the opportunist-human pathogenic fungi progressively increased with pollution levels, in the sequence from green to residential and/or polluted area close to the traffic roads, with highest fraction during winter.Discussion: Hence, our results highlight a close interrelationship between pollution, climatic factors and abundance of certain bacterial and fungal predicted functional groups also with potential implications for human health.

Study on the characteristics of airborne gross alpha and gross beta activities in the vicinity of nuclear facilities

Continuous monitoring of radioactive substances over a prolonged duration can yield crucial insights into the levels of radiation exposure through inhalation, both in the vicinity of nuclear facilities and/or general environments. In this study, we evaluated long-term measurements (2012–2022) of gross alpha-beta activities in the air in the vicinity of nuclear facilities and reference site, distribution characteristics of temporal trends and spatial fluctuations, and factors affecting radioactivity levels. The average airborne gross-α (in mBq m−3) for on-site and off-site were 0.124 and 0.117, respectively, and the average airborne gross-β (in mBq m−3) measurements were 1.10 and 1.04, respectively. The activity ratio (AR) of gross-α and gross-β were calculated as a ratio of 0.12. The distribution characteristics of gross-α and gross-β activities in this study area are likely influenced by the meteorological factors and variations in airborne PM concentrations rather than the operation of the nuclear facility.