To develop a mechanistic constitutive model to characterize the flow structure development in more than one flow regime, the detailed measurement of the flow structure is indispensable. In this study, the void fraction, interfacial area concentration, and bubble velocity profiles of upward air-water two-phase flow has been measured using the four-sensor conductivity probe in a 25.4 mm ID pipe. The test matrix has two features that distinguish it from previous studies, which is the void fraction-based flow condition selection and the wide range. The test matrix consists of 24 flow conditions with 3 different superficial liquid velocities (0.5, 1.0, 2.0 m/s) and 8 different void fractions (0.10, 0.15, 0.20, 0.25, 0.30, 0.35, 0.45, 0.60). The results indicate that the flow characteristics and flow structures not only depend on the void fraction but also significantly depend on the superficial mixture velocity. The velocity effects on various flow characteristics are discussed such as void distribution, cap bubble generation, interfacial area. Besides, a velocity correlation has been developed to predict the bubble velocity radial distribution, with the mean absolute percentage error of 10.05% for the current data.