The paper experimentally and theoretically analyses the velocity profile of air in laboratory stand pipework. Flow velocities measured using a hot-wire anemometer and a vane anemometer were compared with the results of numerical simulation. The k-ε turbulence model was used in the numerical solution of flow rates and for determining the velocity profile using the ANSYS_CFX program. Using power law, this profile was described via an analytical function. Velocities determined by measuring with both instruments in the investigated place in the pipework showed lower values in comparison with the numerical solution. The cause of the difference in velocities was probably inaccurate stating of the volume flow of air through the ultrasonic anemometer. Measurements and simulation showed slight asymmetry in velocity which is related to an insufficiently large volume of the equalisation chamber and the mutual position of the input and output openings.