Agronomic Perspective Research Articles

Manure contribution to rural livelihoods at farm and landscape levels: a systemic approach in semi-arid Central Tunisia

ABSTRACT Manure valorization through on-farm use or market transactions is an ancient and widespread practice in the mixed crop-livestock systems of the semi-arid areas of North Africa. While research has long focused on the manure contribution to soil fertility at the plot level, little has been done concerning livelihood conditions. The present paper aims to assess the contribution of manure use and exchange on the livelihoods of rural communities using an original dataset collected in 2021 among 150 farmers in Central Tunisia. This analysis is carried out within the analytical agroecology framework combined with factor analysis methods. Results showed that manure use and valorization differ along the watershed, from a socioeconomic perspective in small farms operating under rainfed tree-pastoral systems, to an environmental and agronomic perspective in the mixed rainfed-irrigated systems downstream. Manure flow analysis confirmed that on-farm manure balance is positively correlated to economic wealth. However, the manure fluxes questioned the environmental sustainability of the vulnerable zones. Its use and management could significantly impact livelihood discrepancies in the future, with the increasing of demand and use of manure in more favorable zones such as irrigated lands at the detriment of the rainfed zones.

Agronomic perspective: exploring the diversity and potentialities of banana cultivation (Musa spp.)

Agronomic perspective: exploring the diversity and potentialities of banana cultivation (Musa spp.)

Expanding agricultural potential through biological nitrogen fixation: Recent advances and diversity of diazotrophic bacteria

Biological nitrogen fixation (BNF) by diazotrophic bacteria is one of the oldest and most crucial processes in nature. In this process, bacteria form symbiotic associations with plants, capturing atmospheric nitrogen and making it readily available to them. The diversity of nitrogen-fixing bacteria is vast. Recent advancements in molecular biology techniques have enabled the identification of new genera and species capable of fixing nitrogen and providing other types of nutrients for plants. From an agronomic perspective, this process is fundamental in increasing crop productivity sustainably and -cost-effectively. This review aims to categorize the most recent updates on the diversity of nitrogen-fixing bacteria and showcase the main advances in the genetic improvement of legumes for this characteristic. Recent research has revealed a wide diversity of species applicable to various crops of agronomic interest, and many of these bacteria have been used either alone or in consortium with other microorganisms. This study demonstrates the agricultural potential of these new discoveries and the vast possibilities for expanding research into the diversity of microorganisms responsible for BNF in agriculture.

Physiological and Agronomical Response of Coffee to Different Nitrogen Forms with and without Water Stress.

Nitrogen (N) is the most important nutrient in coffee, with a direct impact on productivity, quality, and sustainability. N uptake by the roots is dominated by ammonium (NH4+) and nitrates (NO3-), along with some organic forms at a lower proportion. From the perspective of mineral fertilizer, the most common N sources are urea, ammonium (AM), ammonium nitrates (AN), and nitrates; an appropriate understanding of the right balance between N forms in coffee nutrition would contribute to more sustainable coffee production through the better N management of this important crop. The aim of this research was to evaluate the influences of different NH4-N/NO3-N ratios in coffee from a physiological and agronomical perspective, and their interaction with soil water levels. Over a period of 5 years, three trials were conducted under controlled conditions in a greenhouse with different growing media (quartz sand) and organic soil, with and without water stress, while one trial was conducted under field conditions. N forms and water levels directly influence physiological responses in coffee, including photosynthesis (Ps), chlorophyll content, dry biomass accumulation (DW), nutrient uptake, and productivity. In all of the trials, the plants group in soils with N ratios of 50% NH4-N/50% NO3-N, and 25% NH4-N/75% NO3-N showed better responses to water stress, as well as a higher Ps, a higher chlorophyll content, a higher N and cation uptake, higher DW accumulation, and higher productivity. The soil pH was significantly influenced by the N forms: the higher the NO3--N share, the lower the acidification level. The results allow us to conclude that the combination of 50% NH4-N/50% NO3-N and 25% NH4-N/75% NO3-N N forms in coffee improves the resistance capacity of the coffee to water stress, improves productivity, reduces the soil acidification level, and improves ion balance and nutrient uptake.

Factors influencing fruit cracking: an environmental and agronomic perspective.

Fruit cracking, a widespread physiological disorder affecting various fruit crops and vegetables, has profound implications for fruit quality and marketability. This mini review delves into the multifaceted factors contributing to fruit cracking and emphasizes the pivotal roles of environmental and agronomic factors in its occurrence. Environmental variables such as temperature, relative humidity, and light exposure are explored as determinants factors influencing fruit cracking susceptibility. Furthermore, the significance of mineral nutrition and plant growth regulators in mitigating fruit cracking risk is elucidated, being calcium deficiency identified as a prominent variable in various fruit species. In recent years, precision farming and monitoring systems have emerged as valuable tools for managing environmental factors and optimizing fruit production. By meticulously tracking parameters such as temperature, humidity, soil moisture, and fruit skin temperature, growers can make informed decisions to prevent or alleviate fruit cracking. In conclusion, effective prevention of fruit cracking necessitates a comprehensive approach that encompasses both environmental and agronomic factors.

Characterization of PetM cytochrome b6f subunit 7 domain-containing protein in tomato

Abstract In recent years, multiple advances have been made in understanding the photosynthetic machinery in model organisms. Knowledge transfer to horticultural important fruit crops is challenging and time-consuming due to restrictions in gene editing tools and prolonged life cycles. Here, we characterize a gene encoding a PetM domain-containing protein in tomato. The CRISPR/Cas9 knockout lines of the PetM showed impairment in the chloroplastic electron transport rate (ETR), reduced CO2 assimilation, and reduction of carotenoids and chlorophylls (Chl) under several light conditions. Further, growth-condition-dependent elevation or repression of Chl a/b ratios and de-epoxidation states were identified, underlining possible impairment compensation mechanisms. However, under low light and glasshouse conditions, there were basal levels in CO2 assimilation and ETR, indicating a potential role of the PetM domain in stabilizing the cytochrome b6f complex (Cb6f) under higher light irradiance and increasing its quantum efficiency. This suggests a potential evolutionary role in which this domain might stabilize the site of the Cb6f regulating ratios of cyclic and linear electron transport and its potential importance during the conquest of terrestrial ecosystems during which plants were exposed to higher irradiance. Finally, the results are discussed with regard to metabolism and their implication to photosynthesis from an agronomic perspective.

Does zero tillage save or increase production costs? Evidence from smallholders in Kyrgyzstan

ABSTRACTPromoting zero tillage has been recognized as an important strategy for smallholders from an agronomic perspective. However, the economic effects of adopting zero tillage are still a matter of debate. Employing an endogenous switching regression model on the plot-level panel data of 878 Kyrgyzstan’s smallholders, we investigate the determinants of decision to adopt zero tillage and its effect on smallholders’ production costs. We find that the probability of zero tillage adoption is associated with employment in agriculture, assets, agricultural shocks, fertilizer use, number of plots and average distances from dwelling to household fields and to main road. Furthermore, the results indicate that zero tillage adoption decreases land preparation costs by 23%, but increases hired labour and herbicide costs by 13% and 15%, respectively compared to conventional tillage method. Nevertheless, zero tillage can reduce total production costs by 15%. Our findings suggest that zero tillage can be promoted as an option for resource-scarce smallholders, especially to those in remote areas with poor access to inputs and machinery services. Promoting zero tillage adoption as a labour-saving or herbicide reducing practice can create false expectations among smallholders.

Volatilization or Recovery of Fairway Foliar Nitrogen Fertilizer via Time and Spray Oil Inclusion

Nitrogen (N) is the essential plant nutrient needed by turfgrass in the greatest quantity. Urea and urea-based liquids are arguably the safest, least expensive, and subsequently most popular soluble N fertilizers. Unfortunately, urea fertilizer application to turfgrass is often subject to NH3 volatilization: a deleterious phenomenon from both environmental and agronomic perspectives. The objective of this research was to quantify the efficacy of creeping bentgrass (Agrostis stolonifera L.) golf course fairway foliar fertilization by urea-based N fertilizers as influenced by a petroleum-derived spray oil (PDSO) containing Cu II phthalocyanine colorant (Civitas Turf DefenseTM Pre-M1xed, Intelligro LLC, Mississauga, ON, Canada). In 2019 and 2020, a maintained creeping bentgrass fairway received semimonthly 9.76 kg ha–1 soluble N treatment either alone or in combination with Civitas at a rate of 27 L ha–1. In the 48 h following foliar application, fertilizer N loss as NH3 ranged from 1.3 to 5.5% and corresponded directly to fertilizer urea content but not Civitas inclusion. In the 1 to 14 d following semimonthly treatment, Civitas had either a beneficial (methylol urea and UAN) or negligible (urea) effect on canopy mean dark green color index. Once cumulative N inputs exceeded 47 kg ha–1, creeping bentgrass fairway shoot growth and N nutrition were consistently increased by Civitas complementation of commercial liquid N fertilizer. Over the 2-yr study, absolute mean percent fertilizer N recovery from plots treated by Civitas-complemented foliar liquid N treatment exceeded their ’N only’ counterparts by 8.7%.

Molecular characterization of acetolactate synthase genes in lentil (Lens culinaris Medik.): A key target enzyme of imazethapyr herbicide resistance

Weed infestation severely affects the productivity of lentils. Imazethapyr, a Group 2 herbicide controls prominent broadleaf weeds and grasses in the field. Imazethapyr blocks the first step of branched-chain amino acids (BCAA) biosynthesis by inhibiting the activity of acetolactate synthase (ALS) activity. The present study aims to explore the effect of herbicide stress on the ALS gene expression and enzyme activity in lentil genotypes on different days after spray. The study revealed the presence of two homologous genes in the lentil genome, LcALS2 and LcALS3, present on chromosomes 2 and 3, respectively. qRT-PCR of the LcALS2 gene in the selected herbicide-tolerant (LL1397 and LL1612) and susceptible (FLIP 2004-7 L and PL07) genotypes indicated a significant decrease of the ALS gene expression in the susceptible genotypes. The results were also supported by the in vitro ALS enzyme activity assay, indicating significant inhibition of ALS activity in FLIP 2004-7 L (17.33% and 49.21%) followed by PL07 (33.11% and 19.7%) at 4 and 7 days after spray (DAS), respectively compared to the control. This is the first-ever report on the effect of herbicide stress on ALS gene expression and its correlation with ALS enzyme activity on different days after spraying in lentil crop. It is important from an agronomic perspective to understand herbicide tolerance mechanism at the genetic level for selecting an efficient variety and improving crop productivity.

Measuring Agro-Biodiversity through Leverage Factors: Land Use, Farmer Practices and Public Policies

Understanding the socio-economic channels through which farmers affect agro-biodiversity (ABD) represents a relevant research area, especially in the light of the new sustainable development paradigm. Supporting ABD is one of the main goals of the EU Green Deal and the agricultural sector is being called on for a higher commitment. Nowadays, ABD interventions are linked not only to the genetic and natural resources of species, but also to farming practices, land management, living standards in rural areas and all the policy interventions supporting them. However, the existing literature is still mainly focused on the environmental and agronomic perspectives. This paper aims to assess the extent to which different socio-economic and policy factors affect the performance of farmers in supporting ABD at the territorial level. Starting from the FADN data and focusing on Italy, we built a new regional comprehensive indicator that accounts for three levers through which farmers mostly interact and can influence ABD: land use strategies, agriculture practices and management decisions, and Common Agricultural Policy (CAP) funds. Results reveal a heterogeneous spectrum of indicator scores at the regional level. Land use strategies have a significant relevance for the North-East regions and the Islands (Sicily and Sardinia), whereas agricultural practices emerge as the primary influential factors in the Centre and South. Policy funds dominate in the North-West, reflecting the greater expenditure capacity of the northern regions. These findings have important policy implications for the future of the CAP and the biodiversity EU strategy.

Understanding the impact of terminal heat stress on wheat: Physiological, molecular, and agronomic perspectives

Understanding the impact of terminal heat stress on wheat: Physiological, molecular, and agronomic perspectives

Plantain hybrids for the humid forest agroecology of Central Africa – diseases and pests load, fruit yield and farmers perception

ABSTRACT Plantain is one of the major staples contributing to food security and income generation in West and Central Africa. Local cultivars in Cameroon are susceptible to pests and diseases causing severe losses in plantain production. This study aimed at evaluating the agronomic performance and producer’s perception of plantain hybrids in the humid forest of Cameroon. Field trials were established in a completely randomized block design with eight genotypes and three replicates. Data on pest and disease as well as farmer perception were collected over two growing cycles. These genotypes included seven improved and one local genotype (check). Improved genotypes were highly tolerant to the Black Sigatoka disease compared to local plantain. While root necrosis index was above 50% in local varieties, indices below 25% were recorded in hybrids. Weevil severity in local was higher (55.0 ± 5.2%) compared to 21.0 ± 4.6% to 28.5 ± 3.2% in improved plantains. Average bunch weight was higher for FHIA 21 with 17.9 ± 0.7 kg in the first and 19.7 ± 0.3 kg for the second cycle, while those of the local Ebang were 9.6 ± 0.5 kg and 12.8 ± 0.9 kg, respectively. FHIA 21 and CRBP 568 were the preferred varieties by farmers (68.8% and 56.3% acceptance) from an agronomic perspective. The consumers’ preferences for all the genotypes varied with types of cooking. The implications of these findings for adoption by farmers and consumers as well as for the promotion of the plantain sector in central Africa are discussed.

THE EFFECT OF SPENT MUSHROOM SUBSTRATE ENRICHED WITH SELENIUM AND ZINC ON THE YIELD AND PHOTOSYNTHETIC PARAMETERS OF LETTUCE (Lactuca sativa L.)

The aim of the study was to investigate the influence of Agaricus bisporus spent mushroom substrate (A-SMS) enriched with selenium (Se) and zinc (Zn) on the yield and photosynthetic parameters of lettuce (Lactuca sativa L. var. capitata) of the ‘Skindel’ cultivar. The growing medium for the cultivation of lettuce consisted of A-SMS (10%) and commercial peat (90%). It was further enriched with Se and Zn concentrated at five levels, i.e. 0.1, 0.2, 0.4, 0.6, and 0.8 mmol·L–1 to obtain six growing medias. Se was added to the growing medium in the form of sodium selenite and sodium selenate at a 1:1 ratio, whereas Zn was added in the form of zinc nitrate hexahydrate. Lettuce was grown under controlled conditions in growth chambers. The experiment was conducted in a randomised complete block design in three replicates. The results indicated that the A-SMS added to the growing medium increased both the yield of lettuce and its biological value by increasing the content of Se and Zn. Consumable percent recommended daily allowance and safe hazard quotient for lettuce biofortified with Se and Zn were achieved. The experiment also showed that the addition of Se + Zn did not negatively affect photosynthesis and chlorophyll fluorescence parameters, which proved that these elements did not have toxic effect on lettuce in agronomic perspective.

Host Plant Association and Distribution of the Onion Thrips, Thrips tabaci Cryptic Species Complex.

Simple SummaryOnion thrips, Thrips tabaci Lindeman, 1889, is a key insect pest of several cultivated plant species around the world. Current genetic evidence suggests the existence of three lineages within the species; these lineages are different from each other in several aspects, including reproductive mode, ecological parameters, orthotospovirus transmission efficiency, host plants and distribution range. Despite its importance as a crop pest and the fact that it is one of the most studied thrips species, there is not a comprehensive review of plants in which evidence of breeding occurs among the lineages and the whole species complex. Since identifying the breeding sites of onion thrips has a direct impact on successful pest management strategies, in this paper, we aim to provide a literature review about the host plant association and distribution of the three onion thrips lineages. The results indicate that leek-associated 2 is the most widespread lineage by number of host plants and distribution; leek-associated 1 lineage is primarily found on onion crops and with localised distribution and the tobacco-associated lineage is only reported from tobacco in few locations. In addition, we present a list of host plants for the species, regardless of lineage: 391 plant species from 64 families.Onion thrips, Thrips tabaci Lindeman, 1889 (Thysanoptera: Thripidae) is a pest of economic importance traditionally treated as a polyphagous, cosmopolitan single species. Recent genetic evidence, however, suggests that it is rather a cryptic species complex of three lineages referred to by their host association and displaying different biological and ecological characteristics: leek-associated 1, leek-associated 2 and tobacco-associated. This study reviews host plant associations and distribution of the lineages of this cryptic species complex and discusses its consequences from an agronomical perspective. Overall, leek-associated 2 lineage has the broadest host range, including major crops from different plant families, and it is the only lineage with a confirmed worldwide distribution. Leek-associated 1 lineage shares some host plants with leek-associated 2. It is often found in Allium crops and its geographic distribution is limited to a few dozen countries. Finally, tobacco-associated lineage has only been collected from tobacco and their associated weeds in central and east Europe, and the Middle East. Additionally, this work presents a list of 391 plant species on which breeding and development of T. tabaci occurs, regardless of lineage. These host plant species belong to 64 different families, most importantly Asteraceae, Fabaceae, Brassicaceae, Poaceae, and Solanaceae.

Climate effect on Avena fatua field emergence dynamics: A 38‐year experiment in the semiarid Pampean region of Argentina

Climate events modulate weed population dynamics mainly by influencing field seedling emergence patterns. Field experiments aiming to study the influence of climate on the early-stage establishment of weeds are of utmost importance from a practical and strategic decision-making management point of view. The objective of this study was to analyse the effect of climate variations on the field emergence dynamics of Avena fatua in the southwest area of the semiarid Pampean region of Argentina. Field emergence patterns of A. fatua were monitored from 1977 to 2015. Seedling counts were destructively sampled at weekly intervals. Three quadrats (1 m2 each) were randomly distributed on a 5 ha experimental field with a high natural population density of A. fatua in the absence of a crop. Results show that the emergence strategy of this species is highly plastic with a striking variation in response to year-to-year climate signals. Avena fatua field emergence strategies were classified as staggered, early, medium and late based on both chronological and hydrothermal-time parameters. In the short-term, precipitation regimes during both the after-ripening and emergence phases largely explained the resulting emergence strategy. In the long-term, the combined effect of a reduction in both the precipitation frequency and the mean minimum temperature correlated with an increase in the staggered emergence pattern. Results also point out the adaptability of A. fatua in the area under study, further suggesting a bet-hedging fitness strategy that could diminish the risk of population decline under changing climate scenarios. From an agronomic perspective, the occurrence of staggered emergence patterns with an extended emergence window would complicate the definition of the optimal time for weed control. Thus, tailoring decisions based on the Southern Oscillation Index (SOI) episodes (neutral, negative or positive) forecast plus the implementation of weed emergence models could lead to more accurate and sustainable weed management decisions.

Young and century-old biochars strongly affect nutrient cycling in a temperate agroecosystem

Biochar as a soil amendment is often described as a promising agricultural practice for climate change mitigation and adaptation. However, while recent research showed limited effects of biochar in temperate regions, its long-term impacts on nutrient management in-situ have been overlooked. Here we studied charcoal residues from pre-industrial kiln sites as a proxy to determine the effects of century-old biochar (~200 years old) on nutrient cycles of 3 land covers in a conventionally cropped field. We compared nutrient cycles of soil containing either century-old biochar (CoBC), recently pyrolyzed biochar (YBC) produced from similar feedstock (oak) and amended in similar amounts and a reference charcoal free soil (REF). For these three modalities, we characterized soil chemical properties, the pore water nutrient concentration evolution with time and depth using suction cups, and the crop nutrient uptakes. Our results revealed soil pore water nutrient concentrations strongly depended on biochar age. Indeed, YBC resulted in lower N-NO3- and K+ leaching but higher P-PO4– pore water concentrations in the topsoil Ap (0–30 cm) horizon. In CoBC higher K+ and Mg2+ concentrations occurred in the pore water than in REF for subsoil horizon E (30–60 cm) and Bt (60–100 cm). Beyond soil pore water, CoBC also strongly increased soil total N, available K+ and Ca2+ but decreased available P contents compared to REF and YBC. Finally, although no change in crop productivity occurred, lower N, K, Ca and higher Mg plant uptakes were observed for modalities with biochar. This resulted in no difference in terms of nutrient exports from the field in chicory but it significantly decreased N, K, Ca exports from biochar rich soil under winter wheat in straw. This study delivers the first field-based evidence that the effects of hardwood biochar on nutrient cycles change over its lifetime in a temperate Luvisol soil, whereby young biochar impacts mainly pore water nutrient concentrations and aged biochar mainly plant available contents. In such a strongly managed environment, no differences are noted in productivity despite strong changes in the nutrient cycle. Our study provides insights for addressing long-term effects of biochar in cultivated lands not only in terms of agronomic perspectives but through a biogeochemistry lens.

Current and future agronomic perspectives on rainfed soybean production systems in South Africa

AbstractAlternative crop and soil management practices can offer additional tools to address current soybean [Glycine max (L.) Merr.] yield gaps in South Africa. Despite recent genetic advancements and the introduction of novel weed management strategies, soybean yield remained static. Inconsistent rainfall patterns, high temperatures, and soil compaction have been limiting soybean productivity. Uncertainty exists among farmers regarding optimal nitrogen fertilizer management due to inconsistent yield responses and likely due to a shortage of locally generated scientific information. The benefits provided by including soybean in a maize (Zea mays L.) monoculture production system has been identified, however, crop rotation effects on yield in combination with newly introduced agronomic practices such as no‐tillage and crop residue retention are inconsistent. Modern germplasm offers several opportunities to improve water use efficiency by limiting water‐stress conditions during the reproductive growth stages when yield components are highly sensitive. The introduction of less rigorous soil tillage practices alongside greater residue retention can lessen surface runoff and evaporation losses. Scientifically evaluated research is crucially needed to advance the understanding of current soybean yield gaps and to facilitate the development of tailored management practices to address farm‐specific challenges while beneficial soil and crop conditions are taken advantage of.

The C4 Atriplex halimus vs. the C3 Atriplex hortensis: Similarities and Differences in the Salinity Stress Response

Soil properties and the ability to sustain agricultural production are seriously impaired by salinity. The cultivation of halophytes is seen as a solution to cope with the problem. In this framework, a greenhouse pot experiment was set up to assess salinity response in the perennial C4 species Atriplex halimus, and in the following three cultivars of the annual C3 Atriplex hortensis: green, red, and scarlet. The four genotypes were grown for 35 days with water salinity (WS) ranging from 0 to 360 mM NaCl. Plant height and fresh weight (FW) increased at 360 vs. 0 WS. The stomatal conductance (GS) and transpiration rate (E) were more severely affected by salinity in the C4 A. halimus than in the C3 species A. hortensis. This was reflected in a lower leaf water potential indicating stronger osmotic adjustment, and a higher relative water content associated with more turgid leaves, in A. halimus than A. hortensis. In a PCA including all the studied traits, the GS and E negatively correlated to the FW, which, in turn, positively correlated with Na concentration and intrinsic water use efficiency (iWUE), indicating that reduced gas exchange associated with Na accumulation contributed to sustain iWUE under salinity. Finally, FTIR spectroscopy showed a reduced amount of pectin, lignin, and cellulose under salinity, indicating a weakened cell wall structure. Overall, both species were remarkably adapted to salinity: From an agronomic perspective, the opposite strategies of longer vs. faster soil coverage, involved by the perennial A. halimus vs. the annual A. hortensis cv. scarlet, are viable natural remedies for revegetating marginal saline soils and increasing soil organic carbon.

Key considerations to inform operational EU-specific protection goals: An example for non-target terrestrial plants.

This paper complements recent considerations of specific protection goals (SPG) to inform risk assessments for non‐target terrestrial plants (NTTP) in the European Union. The SPG options in‐field appear to be of the most disruptive potential from agronomic perspective and are therefore investigated in more detail. Overarching prerequisites have been identified that need to be accounted for to ensure that any of the potential SPG options remain operational in a sustainable agricultural context. As soon as crop production is considered a desired ecosystem service for the in‐field, its specific requirements in the context of sustainable agriculture have to be factored in. Good agricultural practices (GAPs), potential ecosystem disservices (e.g. weeds, pests and diseases) and supporting and regulating services need to be considered to ensure a successful and sustainable delivery of the ecosystem service crop production. Concerning in‐field SPG options for NTTP specifically GAPs related to integrated weed management (IWM) require detailed assessment, as they individually and in combination have the purpose of weed control. Therefore, they result in specific implications to the environment, ecosystem services and biodiversity within the context of sustainable agricultural production. When diverging in‐field ecosystem services are considered for the same context, the protection goals options require an additional assessment of synergies and trade‐offs between the relevant ecosystem services (e.g. crop production, climate regulation and aesthetic values), a corresponding weighing and prioritization. Similarly, for biodiversity conservation, the trade‐offs and synergies between sustainable crop production and specific habitat requirements need to be accounted for. Consequently, an interdisciplinary approach can ensure that SPG are operational by integrating a broad understanding of cropping systems, the environmental impact of the tools a farmer uses and the link between habitat availability, the impact of any of the applied tools on habitat quality and the broader landscape context. Integr Environ Assess Manag 2021;17:905–910. © 2021 Bayer AG, BASF SE and Syngenta. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Comparison between barley malt protein quantification methods

Brazil imports about 60% of all the malt used by the brewing industry which is spread throughout the country. Even though it is an agricultural country, only a small portion of the barley production has the characteristics to produce barley malt for brewing. Seeking supply to the ever-growing demand and reducing the dependency on other countries and logistic costs, malting industries and genetic enhancement companies endeavor to adapt barley to the Brazilian Savannah conditions. For the barley to be malted, it is crucial to develop cultivars with the necessary malting qualities and adapted to the region and with good yield. Considering the importance of protein to the brewing process, four protein quantification methods were compared: combustion methods - carbon, hydrogen and nitrogen elemental analysis and Dumas - and digestion ones - Kjeldahl and flux injection analysis (FIA). The methodologies tested showed good correlations (higher than 0.881) and variations were associated with matrix complexity. All protein quantification methods were satisfactory from an agronomic perspective. Yet, we recommend the combustion ones as they generate fewer residues and results are ready in up to 6 minutes. Therefore, they are the most suited for barley and malt analysis considering lab routine.