A field experiment was conducted during kharif season of 2023 at Post Graduate Instructional Farm, College of Agriculture, Pune to study the impact of different phosphorus sources and levels on soybean growth and nutrient uptake in low calcareous soil. The experiment was laid out in randomized block design having eight treatments with three replications. The treatments comprised T1 - Absolute control, T2 - RDF (50:75:45 kg ha-1 N: P2O5: K2O), T3 - 50% P2O5 through PROM, T4 - 75% P2O5 through PROM, T5 - 100% P2O5 through PROM, T6 - 100% P2O5 through DAP + FYM @12.5 t ha-1, T7 - 100% P2O5 through SSP + FYM @12.5 t ha-1 and T8 - 100% P2O5 through vermicompost. The soil of experimental site was clay loam in texture. The findings of the present investigation revealed that the higher nutrient use efficiency was registered in treatment 100% P2O5 through SSP + FYM @12.5 t ha-1 (16.25 kg kg-1). The application of 100% P2O5 through PROM recorded higher nutrient use efficiency (13.31 kg kg-1) over RDF (11.52 kg kg-1). In respect of agronomic nutrient use efficiency, 100% P2O5 through SSP + FYM @12.5 t ha-1 registered higher nitrogen (40 kg grain kg nutrient-1), phosphorus (26 kg grain kg nutrient-1) and potassium efficiency (44 kg grain kg nutrient-1) to soybean crop. The agronomic efficiency of nitrogen, phosphorus and potassium was observed higher in treatment 100% P2O5 through PROM over treatment RDF. After harvest of crop the soil pH was remained unaffected while the electrical conductivity of soil was significantly higher in 100 % P2O5 through vermicompost and PROM to soybean crop. The application of 100% P2O5 either through vermicompost or PROM recorded at par organic carbon content in soil (0.66% and 0.64% respectively). The application of 100% P2O5 through PROM significantly reduced calcium carbonate (6.29%) in soil. The 100% P2O5 through SSP + FYM @12.5 t ha-1 showed higher available nitrogen in soil (297.33 kg ha-1) while the application of 100% P2O5 through DAP + FYM @12.5 t ha-1 exhibited higher level of available phosphorus and potassium content in soil (41.33 and 745.33 kg ha-1, respectively). Further, the application of 100% P2O5 through vermicompost was also significantly superior in available micronutrients like iron (5.7 mg kg-1), manganese (9.9 mg kg-1), zinc (4.6 mg kg-1) and copper (10.4 mg kg-1). The application of 100% P2O5 through PROM registered significantly higher available nitrogen (287.33 kg ha-1), available phosphorus (37.33 kg ha-1), available potassium (737.67 kg ha-1), available micronutrients viz. iron (5.4 mg kg-1), manganese (9.2 mg kg-1), zinc (4.4 mg kg-1) and copper (10.4 mg kg-1) over recommended dose of fertilizers. In general, the integration of organic fertilizers, FYM, PROM and vermicompost, with chemical fertilizers can significantly enhance nutrient use efficiency, soil nutrient content, improve soil health and increase soybean yield in low calcareous soils.