Agriculture-dominated Landscapes Research Articles

A “reserve” of regulating services: The importance of a remnant protected forest for human well-being in the Po delta (Italy)

During the last century the landscape of the Po delta (Italy), one of the most important European deltaic areas, was strongly altered by extended land reclamation and land use intensification. Consequently, the loss of the lowland forested area that was once extended along the region's coast took place. The present study aims to evaluate the ecosystem services provided by the Bosco Mesola woodland, a remnant-forested area of 1,058 ha located on an agriculture-dominated landscape, nowadays protected as a National Nature Reserve. Five ecosystem services were selected and evaluated both in biophysical and monetary terms, including provisioning (firewood production for industrial bioenergy and households’ heating purposes), regulating (air quality and climate regulation by carbon storage), and cultural services (eco-tourism), using experimental and collected data and modeling. The results underlined that the Reserve provides services for a total 10.4 M€ yr-1 and stores carbon for an estimated value of 7.2 M€. Notably, regulating services, such as air quality regulation (i.e. PM10 and O3 removal) accounts for 98.5% of the total annual value. While raising the issue related to the identification and conservation of ESs with no direct market value, whose socio-political and applicative fields have great relevance, this study demonstrates that remnant forests are exceptional providers of ecosystem services that can significantly contribute to reaching multiple sustainable development goals and whose preservation is largely remarked in EU Biodiversity Strategy for 2030. In this regard, the integration of different methods and data sources for ES assessment allow to capture a comprehensive range of ESs that may properly support the decision process.

Rice producer enrollment and retention in a USDA regional conservation partnership program in the southern United States

Private lands conservation is critical to maintain available and quality natural resources in agriculture-dominated landscapes. Financial capital and technical assistance incentives are a primary tool to recruit and retain voluntary participation in private lands conservation programs and, subsequently, to induce persistence of innovative conservation practices. Fundamental to program success is to evaluate how, why, and to what extent incentives and program characteristics motivate participation and persistence. This study draws on diffusion of innovations’ attributes of innovation as our conceptual and interpretive framework to qualitatively explore and describe program participation and persistence of on-farm water, nutrient, and wildlife habitat management practices among a cohort of rice (<i>Oryza sativa</i> L.) producers enrolled in the Rice Stewardship Program (RSP) in the southern United States. A total of 50 interviews were conducted between January of 2019 and July of 2019 in Arkansas, Louisiana, and Mississippi. Findings suggest the program’s lack of complexity (practices were simple to enact) and relative advantage (practices were viewed as better than previous practices) were primary motivations that influenced initial and continued participation, as well as the persistence of specific practices. Compatibility with current on-farm practices and the observability of outcomes or benefits to program participation and its practices were reported consistently but as secondary motivations. Nutrient management practices were observed as having the highest potential persistence as these practices were perceived to be compatible, observable, and relatively advantageous, particularly in relation to the region’s existing nutrient stewardship framework. As few evaluations of private lands conservation programs specific to rice agriculture exist, our findings offer practical insights for managers to consider program evaluation or design that is based on the established innovation attributes framework common to other agricultural contexts.

Land use/land cover classification with Landsat-8 and Landsat-9 satellite images: a comparative analysis between forest- and agriculture-dominated landscapes using different machine learning methods

Land use/land cover classification with Landsat-8 and Landsat-9 satellite images: a comparative analysis between forest- and agriculture-dominated landscapes using different machine learning methods

Response of the rice stem borer Chilo suppressalis (Walker) and its parasitoid assemblage to landscape composition

The rice stem borer (RSB), Chilo suppressalis (Walker), is an economically important pest of rice in subtropical Asia. Up till present, it remains unknown how pest abundance and parasitoid-mediated biological control are modulated by landscape composition. In this study, 20 rice fields with varying proportion of non-crop habitat in (2000 m radius) landscape sectors were selected in China’s Jiangxi province. RSB infestation levels were highest in agriculture-dominated landscapes. Meanwhile, parasitism rate increased with pest pressure but was not related to landscape-level non-crop habitat cover. Landscape-level responses of parasitoids were species-specific and likely modulated by functional traits. The specialist parasitoid Cotesia chilonis (Munakata) responded negatively to non-crop habitat, while positive responses were recorded for the generalists Eriborus sinicus (Holmgren) and Microgaster russata (Haliday). Our work unveils the occurrence of a bottom-up cascade in which spatiotemporal rice cropping patterns shape pest pressure and parasitism dynamics, with more diverse landscapes experiencing lower RSB infestation levels. Non-crop habitats within the landscape matrix equally retained parasitoid species with varying feeding modes and attack strategies. Hence, despite its (globally) inconsistent impacts on biological control, agricultural landscape diversification can directly benefit pest suppression and thus remains a valuable component of the ecological intensification toolbox.

Modelling the Relative Abundance of Roe Deer (Capreolus capreolus L.) along a Climate and Land-Use Gradient.

Simple SummaryThe European roe deer is the most abundant ungulate in Europe. Information on the number of animals and the factors that influence this are essential for the effective management of this species. We developed a model to estimate the pellet group density using transect surveys of dung pellets in the federal state of Bavaria, south-eastern Germany. We used the pellet group density as a proxy for roe deer relative abundance. Our results show that climate, habitat type and wildlife management approach determine the relative number of roe deer. Influential factors differed between seasons and were expected due to changes in food and shelter availability. Although recognized as a woodland species, the majority of roe deer are expected in agriculture-dominated landscapes, which shows their ability to adapt to a wide range of landscape types, especially those with high food availability. Higher numbers were also predicted in regions with intermediate temperatures. Estimates of relative number do not give the actual absolute number of animals but are useful in determining which conditions will have more or fewer animals and can provide information for broad-scale management recommendations. Our results also provide insights into possible future changes in the distribution of relative numbers of roe deer due to climate and land-use change.European roe deer (Capreolus capreolus L.) are important given their economic, recreational and ecological value. However, uncontrolled roe deer numbers can result in negative impacts on forest regeneration and agricultural crops, disease transmission and occurrences of deer-vehicle collisions. Information on the abundance and distribution is needed for effective management. We combined distance sampling (DS) of roe deer dung pellet groups with multiple variables to develop a density surface model (DSM) in the federal state of Bavaria in south-eastern Germany. We used the estimates of pellet group density as a proxy for roe deer relative abundance. We extrapolated our best DSM, conducted a quantitative evaluation and contrasted relative abundance along climate and land-use gradients. Relative abundance of roe deer was influenced by a combination of habitat type, climate and wildlife management variables, which differed between seasons and which reflected changes in food and shelter availability. At the landscape scale, the highest abundance was observed in agriculture-dominated areas and the lowest in urban areas. Higher abundance was also observed in areas with intermediate temperatures compared to the warmest areas. Our results provide information on possible future changes in the distribution of relative abundance due to changes in climate and land-use.

Multilayer landscape classification based on potential vegetation

Vegetation-based landscape classifications reflecting combinations of different types of vegetation promote the understanding of landscape patterns and ecological restoration. However, widespread landscape classifications containing a single thematic resolution may oversimplify landscape patterns. This study aimed at providing a solution for and testing formalized landscape classification, relying on the landscape’s full vegetation potential, i.e. on multiple potential vegetation (MPV). Two areas were studied: the territory of Hungary at a coarse scale and an agriculture-dominated landscape, the Körös-Maros Interfluve (south-eastern Hungary), at a fine scale. Hierarchical clustering and ordination were used to determine landscape types based on potential vegetation type composition of landscapes at each spatial scale. After cutting the resulting dendrogram at several levels and plotting the results on maps and ordination plots, the most relevant thematic resolutions were selected based on the plots and the separation of the groups was tested statistically. The vegetation-based landscape units were reasonably well aligned with biogeographical knowledge at both thematic resolution levels when the study included the whole country. Landscape unit delineation and interpretation based on the typical potential habitats linked to them benefitted from the use of a series of thematic resolutions. For example, in the case of the Körös-Maros Interfluve, apart from well-known grassland vegetation, theMPV-based approach highlighted the distribution of different types of landscape and the potential for woodland in a currently non-wooded area. Furthermore, the finer thematic resolution indicated the possibility of a new type of landscape along temporary small streams. The combined application of clustering and ordination enhanced the interpretation of types of landscape. The use of potential vegetation as an input also enables the classification of currently transformed landscapes. The series of maps with different thematic resolutions allows a flexible choice for specific uses.

Home Range and Space Use of Northern Bobwhite Under Two Different Management Models in Southwestern Missouri

Northern bobwhite (Colinus virginianus; hereafter, bobwhite) management in Missouri, USA has traditionally been focused on providing an interspersion of grass, crop, old field, and woody cover juxtaposed to disked idle areas and food plots to maintain bobwhite populations. This traditional model is implemented with the goal of providing all essential habitat components within 40-acre blocks throughout a larger area used by a population. While this model can produce usable bobwhite space in agriculture-dominated landscapes, it may not be the most effective or efficient approach to producing and maintaining bobwhite in grassland-dominated landscapes. In southwestern Missouri native tallgrass prairie conservation areas are managed primarily with historical ecological processes, such as fire and grazing, to produce the desired patchy habitat mosaic. Additionally, it has been on these native tallgrass prairie conservation areas that managers have seen the most stable and productive bobwhite populations. Over a 5-year period (2014–2018) we quantified movements of northern bobwhite on 3 traditionally managed areas (n = 185) and on 3 managed native tallgrass prairie conservation areas (n = 211) to determine whether home range sizes and space use differed between these two management models. We used the 6-month (Apr–Sep) breeding period to determine core area, home range, mean movement rate, and maximum distance moved. Overall (pooled) home ranges of bobwhite did not differ significantly between traditional and grassland managed areas; however, there were significant yearly differences between management models and study areas. Males generally had larger home range sizes and had higher movement rates than females. For the 5 years of the study few birds made long-distance movements (>1.6 km; 3%), and all remained relatively close to capture locations in winter (Feb–Mar). For both traditional and grassland managed areas, bobwhite selected for areas that had disturbance (fire and grazing) in the last 2 years and for the native grassland vegetation type. These findings suggest that areas managed under the grassland management model provide preferred habitat for bobwhite and could result in significant improvement in habitat quality for tallgrass prairie wildlife.

Carbon sequestration by forests and agroforests: a reality check for the United States

Climate change is a major global threat affecting food security and sustainability. Land use systems involving trees have the potential to positively impact climate change by reducing atmospheric carbon dioxide (CO2) and providing long-term carbon (C) storage. This review evaluated the C sequestration potential of two major land use systems of the United States (US) involving trees, forests and agroforests, which can also provide other ecosystem services.The estimated total forest C stock on forest land in the US in 1990 was 50,913 Tg and another 1885 Tg remained in harvested wood and discarded wood products. From 1990 to 1995, total C stock rose by 2%, and from 2000 to 2005, it rose by 1.7%. The US forests collectively lose (flux) about 200 Mg C y-1 from disturbance and harvesting. Currently, about 12% of the conterminous US forest land is at high or very high risk of wildfire. Annually, insects and diseases could transfer ~ 21 Tg of live aboveground biomass to litter and woody debris pools. A scenario that targets an afforestation policy for rural landowners in the eastern US and a reforestation policy targeting understocked federal forest lands in the western would improve US annual sequestration compared to the baseline of 323 Tg CO2 eq yr-1 in 2015 to 469 Tg CO2 eq yr-1 in 2050.Agroforestry offers greater potential to increase C sequestration of predominantly agriculture-dominated landscapes than monocrop agriculture by storing C in above- and belowground biomass, soil, and living and dead organisms and further extending the duration of C in soils. The estimated total C sequestration of current alley cropping (211,938 ha), riparian buffers (640,732 ha), silvopasture (34 Mha), and windbreak (2.37 Mha) practices is 219 Tg C yr-1. The total C sequestration would be 240 Tg C yr-1 with 5% of the US cropland converted to alley cropping (3.7 Tg yr-1), 15-m wide riparian buffers on both sides of 5% of the total stream length (4.75 Tg yr-1), 34 Mha converted to silvopasture (207 Tg yr-1), and windbreaks on 5% (7.45 Mha) of the cropland (25 Tg yr-1). Despite many limitations including uncertainty of land areas under agroforestry, lack of standardized estimation protocols, and lack of accountability on various C stocks (source-sink services, detritus C, insect/pest damages, etc.), we believe these new accrual rates and the land areas under each practice are much more realistic as new information became available over the last decade.The total C sequestration by forests (776) and agroforests (219) is 995 Tg yr-1 and represents approximately 15% of the US CO2 emissions. This review highlights the importance of sustainable management of forests and integration of agroforestry on agricultural lands to mitigate climate challenges further while meeting society’s need for food and a healthy environment.

Balancing conservation priorities for grassland and forest specialist bird communities in agriculturally dominated landscapes

Effective conservation planning often requires difficult decisions when at-risk species inhabit economically valuable landscapes or if the needs of multiple threatened species do not align. In the agriculture-dominated landscape of eastern Ontario and southwestern Quebec, Canada, conflicting habitat requirements exist between threatened grassland birds benefiting from certain agriculture practices, and those of a diverse woodland bird community dependent on forest recovery. Using multi-scale species distribution models with data from the North American Breeding Bird Survey (BBS), we assessed habitat suitability for 8 threatened grassland and forest specialists within this region. We also identified landscapes that jointly maximize occurrence of the 8 focal species and diversity of the full avian community. Influential habitat associations differed among species at the territory (200 m radius) and landscape level (1 km), highlighting the importance of considering multiple spatial scales. Species diversity was maximized when forest or grassland/pasture cover approached 40–50%, indicating a positive response to land cover heterogeneity. We identified species diversity hotspots near Lake Huron, as well as along the shore and southeast of the St. Lawrence River. These areas represent mosaic landscapes, balancing forest patches, wetland, grassland/pasture, and row crops such as corn, soybean, and cereals. Despite drastic landscape changes associated with agroecosystems, we demonstrate that targeted habitat protection and enhancement that prioritizes land cover diversity can maximize protection of bird communities with directly contrasting needs. We highlight multiple pathways to achieve this balance, including forest retention or separating row crops with hedgerows and wooded fence-lines, improving flexibility in conservation approaches.

Complex Small-Holder Agriculture in Rainforest Buffer Zone, Sri Lanka, Supports Endemic Birds

The Sinharaja rainforest in southwestern Sri Lanka is a protected forest in a largely agriculture-dominated landscape. In keeping with global UNESCO global biosphere reserves planning, the Sinharaja is surrounded by a buffer zone of regenerating forest and villages with small tea plots and multi-strata tree gardens (homegardens). Globally, however, conservation planning lacks standards on buffer zone management. We ask what relationships exist between village land use and bird assemblages, which are effective ecosystem indicators. Birds have been little studied across land use and vegetation structure in actively managed, large, protected forest buffer zones. To that end, we ran spatially- and temporally-replicated bird point counts across tree gardens, forest fragments, and tea plots within a Sinharaja village. Tree gardens held a greater abundance of birds across habitat association, conservation concern, diet, and endemic species than forest fragments or tea plots. Forest fragments and tree gardens hosted statistically similar numbers of birds in some subsets, but their species assemblages differed. In tea plots, greater shade tree species richness correlated with greater bird abundance and species richness. Our results support the argument for programs to support complex small-scale tree-based agroforestry embedded in buffer zone regenerating forest.

Factors affecting crop field use by Blackbuck in Krishnasaar Conservation Area, Nepal.

Abstract Reducing crop damage caused by wildlife in forest-agricultural land interface requires an understanding of the spatial pattern of crop field use and its drivers. We assessed factors influencing the spatial pattern of crop-field use by a generalist wild herbivore, blackbuck ( Antilope cervicapra ), in the agriculture-dominated landscape of Krishnasaar Conservation Area (KrCA) in low land Nepal. We systematically selected 103 crop fields distributed over the entire KrCA (within both core area and community development zone) and surveyed them three times over three months, resulting in 309 sampling occasions. We found that crop use by blackbuck was highly localized inside core area (only 15% of the sampled crop fields were used) even though crop fields outside the core area were accessible and had limited mitigation measures applied. The linear mixed effect modeling showed that probability of crop field use by blackbuck increased with size of the crop field, implying the magnitude of foraging benefits affects blackbuck crop-use decisions. The presence of current mitigation measures (e.g., scare crow, vegetative fencing) did not have discernible influence on probability of crop field use, so did the types of the crops, and distance of crop fields from the center of the core area. Overall, our results suggest that generalist herbivore, blackbuck, employs low gain-low risk strategy when using crop fields in natural habitat-agricultural. Minimizing current level of crop damage by blackbuck will require working closely with farmers who owns lands close to the natural habitat (core area) to apply appropriate mitigation measures.

Modeling the ecosystem services of native vegetation management practices at solar energy facilities in the Midwestern United States

The increasing pressure on land resources for food and energy production along with efforts to maintain natural systems necessitates the development of compatible land uses that maximize the co-benefits of multiple ecosystem services. One such land sharing opportunity is the restoration and management of native grassland vegetation beneath ground-mounted solar energy facilities, which can both protect biodiversity and restore related ecosystem services. In this paper, we applied the InVEST modeling framework to investigate the potential response of four ecosystem services (carbon storage, pollinator supply, sediment retention, and water retention) to native grassland habitat restoration at 30 solar facilities across the Midwest United States. Compared to pre-solar agricultural land uses, solar-native grassland habitat produced a 3-fold increase in pollinator supply and a 65% increase in carbon storage potential. We also observed increases in sediment and water retention of over 95% and 19%, respectively. We applied these results to project the potential benefits of adoption of native grassland management practices in current and future solar energy buildout scenarios. Our study demonstrates how multifunctional land uses in agriculture-dominated landscapes may improve the provision of a variety of ecosystem services and improve the landscape compatibility of renewable energy and food production.

Forest Area Change in the Shifting Landscape Mosaic of the Continental United States from 2001 to 2016

The landscape context (i.e., anthropogenic setting) of forest change partly determines the social-ecological outcomes of the change. Furthermore, forest change occurs within, is constrained by, and contributes to a dynamic landscape context. We illustrate how information about local landscape context can be incorporated into regional assessments of forest area change. We examined the status and change of forest area in the continental United States from 2001 to 2016, quantifying landscape context by using a landscape mosaic classification that describes the dominance and interface (i.e., juxtaposition) of developed and agriculture land in relation to forest and other land. The mosaic class changed for five percent of total land area and three percent of total forest area. The least stable classes were those comprising the developed interface. Forest loss rates were highest in developed-dominated landscapes, but the forest area in those landscapes increased by 18 percent as the expansion of developed landscapes assimilated more forest area than was lost from earlier developed landscapes. Conversely, forest loss rates were lowest in agriculture-dominated landscapes where there was a net loss of five percent of forest area, even as the area of those landscapes also increased. Exposure of all land to nearby forest removal, fire, and stress was highest in natural-dominated landscapes, while exposure to nearby increases in developed and agriculture land was highest in developed- and agriculture-dominated landscapes. We discuss applications of our approach for mapping, monitoring, and modeling landscape and land use change.

Use of hedgerows by mammals in an intensive agricultural landscape

Agricultural intensification causes habitat modification, sometimes leading to habitat loss and subsequent loss of connectivity. Remaining species in these agriculture-dominated landscapes often use hedgerows, such as windbreaks or riparian strips, as movement corridors or even as habitats. However, the understanding of the use of these hedgerows by mammals is limited and could be improved with the use of high-resolution remote sensing data, which are unbiased, detailed and repeatable. The aim of this study was to assess the attributes that affect medium- and large-sized mammals’ use of hedgerows, with in situ and remotely sensed data (including LiDAR and multispectral images) in an agriculture-dominated landscape in southern Québec. Twenty-three hedgerows were selected and characterized with both field surveys and remote sensing analyses, like LiDAR metrics and vegetation indices. Wildlife frequentation of each hedgerow was measured using camera traps, from late spring to early fall in 2018. 431 mammal detections were obtained among all 23 hedgerows. From this, seven species were recorded, all of them opportunistic and well adapted to agricultural environment. Results showed significant differences in mammal use of hedgerows. Coefficients of the better-ranked models based on AICc indicated a positive relationship between hedgerow length and their use by mammals, and a negative relationship with the hedgerow width. Hedgerow use by mammals also increased as tree cover and understory density increased, and as human disturbance decreased. These results characterized for the first time the variables influencing hedgerow use by a broad set of medium- and large-sized mammal species and confirmed their use as movement corridors and/or habitat. This study also confirmed the complementary usefulness of variables derived from remote sensing combined with field data. The low explanatory power of variables often cited in the literature (e.g. NDVI, gappiness) also highlights the need to further explore their specific influence on mammals. The information provided by this study supports the beneficial role played by hedgerows for wildlife conservation in intensive agricultural landscapes. Management guidelines are provided as well as future research avenues.

Effects of Field and Landscape Scale Habitat on Insect and Bird Damage to Sunflowers

Agriculture-dominated landscapes harbor significantly diminished biodiversity. Woody vegetation along field margins can provide farmers with ecosystem services and benefit biodiversity. However, when crops are damaged by the biodiversity harbored in such vegetation, farmers are reluctant to incorporate field margin habitat onto their land and may even actively remove such habitats. We investigated how damage by both insect pests (sunflower moth, Homoeosoma electellum) and avian pests to sunflower (Helianthus annuus) seed crops varied as a function of field-margin and landscape-scale habitat, as well as by bird abundance and diversity. Surveys for insect damage, avian abundance, and bird damage were carried out over two years in 30 different fields. The mean percentage of moth-damaged sunflowers sampled was nearly four times higher in fields that had bare or weedy margins (23.5%; $877/ha) compared to fields with woody vegetation (5.9%; $220/ha) and decreased in both field types as landscape-scale habitat complexity declined. Birds damaged significantly fewer sunflower seeds (2.7%) than insects, and bird damage was not affected by field margin habitat type, landscape-scale habitat variables, or avian abundance, but was significantly higher along field edges compared to  50m from the field edge. Avian species richness nearly doubled in fields with woody margin habitat compared to fields with bare/weedy margins in both the breeding season and in fall. These results indicate that the benefits of planting or retaining woody vegetation along sunflower field margins could outweigh the ecosystem disservices related to bird damage, while simultaneously increasing the biodiversity value of intensively farmed agricultural landscapes.

Weather effects on birds of different size are mediated by long-term climate and vegetation type in endangered temperate woodlands.

Species occurrence is influenced by a range of factors including habitat attributes, climate, weather, and human landscape modification. These drivers are likely to interact, but their effects are frequently quantified independently. Here, we report the results of a 13-year study of temperate woodland birds in south-eastern Australia to quantify how different-sized birds respond to the interacting effects of: (a) short-term weather (rainfall and temperature in the 12months preceding our surveys), (b) long-term climate (average rainfall and maximum and minimum temperatures over the period 1970-2014), and (c) broad structural forms of vegetation (old-growth woodland, regrowth woodland, and restoration plantings). We uncovered significant interactions between bird body size, vegetation type, climate, and weather. High short-term rainfall was associated with decreased occurrence of large birds in old-growth and regrowth woodland, but not in restoration plantings. Conversely, small bird occurrence peaked in wet years, but this effect was most pronounced in locations with a history of high rainfall, and was actually reversed (peak occurrence in dry years) in restoration plantings in dry climates. The occurrence of small birds was depressed-and large birds elevated-in hot years, except in restoration plantings which supported few large birds under these circumstances. Our investigation suggests that different mechanisms may underpin contrasting responses of small and large birds to the interacting effects of climate, weather, and vegetation type. A diversity of vegetation cover is needed across a landscape to promote the occurrence of different-sized bird species in agriculture-dominated landscapes, particularly under variable weather conditions. Climate change is predicted to lead to widespread drying of our study region, and restoration plantings-especially currently climatically wet areas-may become critically important for conserving bird species, particularly small-bodied taxa.

Transition of Ethiopian highland forests to agriculture-dominated landscapes shifts the soil microbial community composition

BackgroundLand use changes and related land management practices significantly alter soil physicochemical properties; however, their effects on the soil microbial community structure are still unclear. In this study, we used automated ribosomal intergenic spacer analysis to determine the fungal and bacterial community composition in soils from different land use areas in the Ethiopian highlands. Soil samples were collected from five areas with different land uses, natural forest, eucalyptus plantation, exclosure, grassland and cropland, which had all historically been natural forest.ResultsOur results showed a significant shift in the soil bacterial and fungal community composition in response to land use change. We also identified soil physicochemical factors corresponding to the changes in bacterial and fungal communities. Although most soil attributes, including soil organic carbon, total soil nitrogen, labile P, soil pH and soil aggregate stability, were related to the change in bacterial community composition, the total soil nitrogen and soil organic carbon had the strongest relationships. The change in fungal community composition was correlated with soil nutrients, organic carbon, soil nitrogen and particularly the labile P concentration.ConclusionsThe fungal community composition was likely affected by the alteration of vegetation cover in response to land use change, whereas the bacterial communities were mainly sensitive to changes in soil attributes. The study highlights the higher sensitivity of fungal communities than bacterial communities to land use changes.

Landscape context is more important than wetland buffers for farmland amphibians

In regions with high rates of wetland loss, remnant wetlands and constructed ponds can provide important breeding habitat for amphibians. However, such wetlands are often embedded in a matrix of agricultural fields, potentially putting species within these wetlands at risk. One recommendation for conservation of amphibians in agriculture-dominated landscapes is to maintain a buffer of permanent vegetation around the wetland. However, it is not clear how wide wetland buffers must be to effectively conserve amphibians in agriculture-dominated landscapes or what vegetation types are suitable buffer vegetation. Furthermore, it is not clear whether wetland buffers produce similar—or better—conservation outcomes for amphibians than actions conducted at larger spatial extents. We addressed these questions using data from anuran (frog and toad) breeding call surveys in 36 wetlands in rural eastern Ontario, Canada. First, we tested for the effects of 49 different wetland buffer measurements on anuran richness, relative anuran abundance, and the abundance/probability of occurrence of individual species. These 49 measurements represented all combinations of seven different ways to measure the wetland buffer size and seven types of buffer vegetation. Wetland buffer size was measured as the minimum width of buffer vegetation contiguous with the wetland and proportion of the area within 5, 16, 30, 50, 120, or 300 m of the wetland containing buffer vegetation that was contiguous with the wetland. Then, to compare the strength of effect of wetland buffers versus landscape context on anurans, we compared the wetland buffer measurement with the strongest positive effect on each anuran response (from the previous analysis) to the effects of three landscape-scale variables: area of woodland; area of wetlands, streams, rivers, and lakes; and road density. We did not detect positive effects of wetland buffers on anuran richness or relative anuran abundance. This is because positive effects of wetland buffers on individual species were rare, i.e. positive effects were only supported for two of the six species with enough data to model individually: American toads (Anaxyrus americanus) and green frogs (Lithobates clamitans). Furthermore, we found that the landscape context had much stronger effects on relative anuran abundance than the wetland buffer, with effect sizes ranging from 4 (road density) to 14 (woodland cover) times that of the wetland buffer. These findings suggest that guidelines for anuran conservation in agricultural landscapes should generally focus on protection of terrestrial habitat at the landscape scale rather than on maintenance of wetland buffers.

Landscape-scale effects of land use intensity on birds and butterflies

Although today there is ample evidence that biodiversity is affected by agricultural land use intensification, little is known about how species respond to different land use intensity gradients at landscape scale. To properly describe the relationship between biodiversity and land use intensity, intensity indicators need to account for land cover, management intensity, and be assessed at landscape scale. The study was conducted in 91 landscapes of 1 km2 in Switzerland. Three different land use intensity indicators were calculated: indicator 1 was defined as the ratio between agricultural and natural area; indicator 2 as the ratio between arable land and permanent grassland; and indicator 3 as the ratio between agricultural area and biodiversity promotion areas (BPA, i.e. wildlife-friendly managed areas under Swiss agri-environment schemes). Species richness and abundance of birds and butterflies were used as biodiversity indicators and trait-based community indices were used to describe bird community changes. Overall, we found that birds were affected by landscape composition and agricultural management, while butterflies were mainly affected by agricultural management. Specifically, from natural (e.g. forest dominated) to agriculture-dominated landscape, bird species richness showed a sharp decrease when 80% or more of the landscape was farmed. Butterfly species richness followed a hump-shaped curve. None of the species groups was significantly correlated with the proportion of arable land versus permanent grassland. Yet species richness of birds and butterflies significantly changed with the proportion of BPA: the lower the proportion of BPA, the lower the observed richness. Finally, when the proportion of agricultural land increased, populations of migratory birds and hedge/tree breeders decreased. We conclude that to further promote farmland biodiversity, natural areas, such as forests, hedges and waterbodies, should cover at least 20% of the agricultural landscapes and the proportion of BPA should be increased.

SURVEILLANCE FOR HEMORRHAGIC SEPTICEMIA IN BUFFALO (BUBALUS BUBALIS) AS AN AID TO RANGE EXPANSION OF THE JAVAN RHINOCEROS (RHINOCEROS SONDAICUS) IN UJUNG KULON NATIONAL PARK, INDONESIA

The Javan rhinoceros ( Rhinoceros sondaicus) of Ujung Kulon National Park (UKNP) is the crown jewel of Indonesia's rich natural history. The park lies on a peninsula surrounded by coastline and agriculture-dominated landscapes. The invasion of water buffalo ( Bubalus bubalis) into the park carries a substantial health risk to the Javan rhinoceros and threatens plans to establish a new population outside of its only current range in UKNP. Hemorrhagic septicemia (HS), known locally as septicemia epizootica and caused by Pasteurella multocida B:2, could thwart Indonesia's efforts to expand the range of the Javan rhinoceros. Because HS was considered eradicated from Banten Province, few preventative programs have been available to farmers. During June 2012-July 2013, biologic samples were collected from 770 water buffalo in 19 villages. Deep nasal swabs ( n=85) were taken for bacterial culture and blood samples ( n=770) were collected for serologic testing. No animals were positive on culture. The prevalence of antibody to P. multocida in this population was 1.8% (14 of 770 animals). A structured questionnaire was used to gather information about possible risk factors. Husbandry practices associated with presence of antibody in water buffalo included lack of a permanent area to house buffalo at night, low body condition score (=2), high body temperature (≥40 C), a history of clinical signs or sudden death in the previous year, and a grazing system that utilized significant forage inside the park. Antibody was not associated with sex, age, vaccination status, or season. Understanding HS disease dynamics in the buffalo adjacent to UKNP may improve the livelihoods of people and health of endangered rhinoceroses in this ecosystem.