Agricultural Use Research Articles

Mulching in irrigated forage cactus promotes early harvest and improves the economic indices of the crop

AbstractTo mitigate the negative effects of climate, the use of resilient agriculture is essential. This study evaluated the impact of different levels of biotic mulch on irrigated forage cactus yield, phenology and economic indices. The ‘Orelha de Elefante Mexicana’ clone was used and subjected to four levels of biotic mulch—BM (0, 5, 10 and 15 Mg ha−1) in a randomised block design with four repetitions. The treatments consisted of growing forage cactus with 0, 5, 10 and 15 Mg ha−1 mulch. The experiment was carried out in a semi‐arid environment in Brazil (Serra Talhada, Pernambuco State) over two successive cycles (August 2020 to August 2022). The yield, cutting time, and morphophysiological, economic and water use indicators were evaluated. There was no effect of the BM level on the final forage yield. In the first experimental cycle, the treatment with 10 Mg ha−1 BM brought the cutting time forward by 103°C day. The application of 15 Mg ha−1 BM prolonged phenophase 2, causing an increase of 236°C day in the cycle compared with the treatment with 10 Mg ha−1 BM. We concluded that mulch improved crop water and economic indicators, contributing to sustainable forage production in semiarid environments.

Advancing Water Security and Agricultural Productivity: A Case Study of Transboundary Cooperation Opportunities in the Kabul River Basin

The Kabul River Basin (KRB) is witnessing frequent flood and drought events that influence food production and distribution. The KRB is one of the world’s poorest regions regarding food security. Food security issues in the KRB include shifts in short-term climate cycles with significant river flow variations that result in inadequate water distribution. Due to the lack of hydro-infrastructure, low irrigation efficiency, and continuing wars, the Afghanistan portion of the KRB has experienced low agricultural land expansion opportunities for food production. This research assesses the relationship between flood mitigation, flow balances, and food production and, cumulatively, assesses the social and economic well-being of the population of the KRB. SWAT modeling and climate change (CCSM4) implications are utilized to assess how these relationships impact the social and economic well-being of the population in the KRB. The intricacies of transboundary exchange and cooperation indicate that the conservation of ~38% of the water volume would nearly double the low flows in the dry season and result in the retention of ~2B m3/y of water for agricultural developmental use. Results show that the peak flood flow routing in reservoirs on the Afghanistan side of the KRB would have a substantial positive impact on agricultural products and, therefore, food security. Water volume conservation has the potential to provide ~44% more arable land with water, allowing a ~51% increase in crop yield, provided that improved irrigation efficiency techniques are utilized.

Agroforestry for Carbon Neutrality: An Effective Pathway to Net Zero

Agroforestry is a promising strategy to achieve carbon neutrality and net zero emission, aiming towards the balance between greenhouse gas emissions and equal amount of carbon removal through sequestration Among several methods of carbon sequestration, agroforestry stands out for its unique ability to simultaneously sequester carbon while reducing greenhouse gas emissions, particularly nitrous oxide (N2O) emissions associated with chemical fertilizer use in conventional agriculture. These systems store carbon through multiple pathways like, in above-ground biomass, below-ground root systems, and enhanced soil organic carbon accumulation while maintaining the agricultural productivity. Acknowledged under the afforestation and reforestation programs of the Kyoto Protocol, agroforestry has attracted interest for its several advantages from both industrialized and developing countries for its multifaceted benefits, including its potential to combat desertification and reduce anthropogenic emissions. Beyond carbon sequestration, it enhances soil fertility, supports biodiversity conservation, and provides economic diversification for farmers through multiple income streams. The role of REDD+ (Reducing Emissions from Deforestation and Forest Degradation) negotiations in extending the Kyoto Protocol's second commitment period has further strengthened agroforestry's position in global climate action, particularly through the development of REDD offset credits in compliance carbon markets. The World Agroforestry Centre defines agroforestry as a dynamic, ecologically based natural resources management system that integrates trees on farms and in the agricultural landscape, diversifying and sustaining production for increased social, economic, and environmental benefits. The Association for Temperate Agroforestry (AFTA) defines it as an intensive land management system that optimizes the benefits from biological interactions created when trees and/or shrubs are deliberately combined with crops and/or other natural resources. This review article critically examines agroforestry's crucial role as an effective pathway toward achieving carbon neutrality and net zero goals, synthesizing current knowledge and future prospects.

Agricultural waste-based lactic acid production by the fungus Rhizopus oryzae: a tool for sustainable polylactic acid production for agricultural use - a review

Agricultural waste-based lactic acid production by the fungus Rhizopus oryzae: a tool for sustainable polylactic acid production for agricultural use - a review

Soil Suitability Evaluation for Agriculture: New Model Development and Validation

ABSTRACT The evaluation of land suitability for agricultural production is a complex process that requires specialized information and expert analysis. This paper introduces the Soil Suitability Evaluation (SSE) model, a computer program designed to assess land suitability for agriculture. The model considers soil surface conditions (erosion, micro-relief, and slope), physical characteristics (texture and depth), and chemical properties (salinity, sodicity, fertility, and calcium carbonate). It involves calculating these soil characteristics along with their respective weights. Compared to other land suitability evaluation methods, the SSE model provides realistic and practical results including assessments of soil and crop suitability. It serves as a simple and useful tool for decision-makers to determine land quality for agricultural use and proposed some recommendations for land reclamation.

Effects of chitosan on plant growth under stress conditions: similarities with plant growth promoting bacteria

The agricultural use of synthetic pesticides, fertilizers, and growth regulators may represent a serious public health and environmental problem worldwide. All this has prompted the exploration of alternative chemical compounds, leading to exploring the potential of chitosan and PGPB in agricultural systems as a potential biotechnological solution to establish novel agricultural production practices that not only result in fewer adverse impacts on health and the environment but also improve the resilience and growth of the plants. In this work, an analysis of the impact of plant growth-promoting bacteria (PGPB) and chitosan on plant growth and protection has been conducted, emphasizing the crucial bioactivities of the resistance of the plants to both biotic and abiotic stressors. These include inducing phytohormone production, mobilization of insoluble soil nutrients, biological nitrogen fixation, ethylene level regulation, controlling soil phytopathogens, etc. Moreover, some relevant aspects of chitin and chitosan are discussed, including their chemical structures, sources, and how their physical properties are related to beneficial effects on agricultural applications and mechanisms of action. The effects of PGPB and chitosan on photosynthesis, germination, root development, and protection against plant diseases have been compared, emphasizing the intriguing similarities and synergistic effects observed in some of these aspects. Although currently there are limited studies focused on the combined application of PGPB and chitosan, it would be important to consider the similarities highlighted in this work, and those that may emerge in future studies or through well-designed investigations, because these could permit advancing towards a greater knowledge of these systems and to obtain better formulations by combining these bioproducts, especially for use in the new contexts of sustainable agriculture. Thus, it seems feasible to augur a promising near future for these combinations, considering the wide range of possibilities offered by chitinous biomaterials for the development of innovative formulations, as well as allowing different application methods. Likewise, the studies related to the PGPB effects on plant growth appear to be expanding due to ongoing research to test on plants the impacts of microorganisms derived from different environments, whether known or recently discovered, making it a very exciting field of research.

Internet Use in Agriculture and Farm Earnings: An Analysis of the Indonesia Labor Force Survey

Internet Use in Agriculture and Farm Earnings: An Analysis of the Indonesia Labor Force Survey

Unraveling the genetic and physiological potential of donkeys: insights from genomics, proteomics, and metabolomics approaches.

Donkeys (Equus asinus) have played a vital role in agriculture, transportation, and companionship, particularly in developing regions where they are indispensable working animals. The domestication of donkeys marked a significant turning point in human history, as they became essential for transportation, agriculture, and trade, especially in arid and semi-arid areas where their resilience and endurance were highly valued. In modern society, donkeys are indispensable due to their diversified applications, including meat, dairy, medicine, and functional bioproducts, supporting economic, cultural, and medical industries. Despite their critical importance, research on donkeys has historically been overshadowed with studies on horses. However, recent advancements in high-throughput sequencing and bioinformatics have significantly deepened our understanding of the molecular landscape of donkey genome, uncovering their unique adaptations, genetic diversity, and potential therapeutic applications. Microsatellite and mitochondrial DNA (mtDNA) markers have proven effective in assessing the genetic diversity of donkeys across various regions of the world. Additionally, significant strides have been made in characterizing differentially abundant genes, proteins, and metabolic profiles in donkey milk, meat, and skin, and in identifying specific genes/proteins/metabolites associated with sperm quality, motility, and reproduction. Advanced genomic technologies, such as genome-wide association studies and the identification of selection signatures, have also been instrumental in delineating genomic regions associated with phenotypic and adaptive traits. This review integrates data from diverse studies, including those on genetic diversity, transcriptomics, whole genome sequencing, protein analysis, and metabolic profiling, to provide a comprehensive overview of donkey biology. It underscores the unique characteristics of donkeys and emphasizes the importance of continued research to improve their genetic management, conservation, and agricultural use, ensuring their ongoing contribution to human societies.

Exploring the Interactions of Climate Change, Freshwater Supply, and Environmental Balance

Climate change has far-reaching implications for global freshwater availability and environmental balance. The increase in greenhouse gases from the burning of fossil fuels has triggered global climate change, affecting precipitation patterns, glacier melt and sea level rise. Parts of the United States are experiencing significant changes in precipitation patterns, with some areas experiencing frequent storms and others experiencing more prolonged droughts. Accelerated glacier melt is leading to fewer water sources during the dry season, and sea level rise is threatening coastal freshwater resources, triggering saltwater intrusion and a decline in freshwater quality. Wildfires in California are an important consequence of climate change, severely affecting freshwater resources and environmental balance. Wildfires lead to reduced water resources, impacting agricultural water use and irrigation infrastructure, and affecting water quality through increased sediment and pollutant loads. The 2018 Camp Hill Fire contaminated local water sources, and the rebuilding process has increased demand for water resources, exacerbating resource constraints. Global warming has lengthened wildfire seasons and increased fire intensity, leading to increased pressure on freshwater resources. By analyzing the impacts of climate change on freshwater resources, this paper hopes to draw public attention to environmental protection and provide a reference for gradually optimizing ecosystems and creating a healthy living environment.

Increasing Yield more than Maintaining Fertile Soils Motivated the Choice of Climate-Smart Soil Technologies among Greater Lira Pigeon Pea Farmers

Pigeon pea farmers in Gerater Lira, Uganda, fight the adverse effects of climate change and deteriorating soil fertility. They are combining different strategies and adopting climate-smart soil technologies (CSS technologies) to maintain fertile soil and increase yields. The study explored how the motive for maintaining fertile soils affected CSS technologies' choice. A sample of 39 farmers participated in laddering interviews. Data was analyzed by the means-end chain (MEC) framework and the centrality index (CI) technique. MEC results indicate that farmers predominantly linked crop diversification, addressed dietary needs, increased yields, and increased incomes. In addition, they paid less attention to maintaining fertile soils. Results of the CI highlight goal priorities by gender subgroups with females aged at least 40 paying more attention to producing food, soil fertility, and improving health, while male farmers of the same age category were inclined to spread production risk. Results further showed that male farmers below 40 years of age tend to produce for markets and benevolent, while their female counterparts tend to maintain soil nutrients. Our overall findings could help in the development of targeted strategies to encourage a wider spread of CSS technology use for climate-smart agriculture. This could enhance agricultural resilience in the face of climate change. We recommend encouraging farmers to apply CSS technologies while considering the long-term effects they might have on soil fertility. we further recommend that farmers intensify residual retention to improve soil fertility without requiring money to purchase inorganic fertilizer.

Combined Effects of Magnetized Irrigation and Water Source on Italian Lettuce (Lactuca sativa L. var. ramosa Hort.) Growth and Gene Expression

Agricultural water scarcity has become a global issue. Optimizing irrigation water quality and effectively utilizing non-conventional water resources are essential strategies to alleviate pressure on agricultural water use and achieve sustainable development. This study employed Italian lettuce as the test crop to explore the effects of magnetization treatment (M) at a magnetic field strength of 0.2 T and various irrigation water sources (T) on its growth. The following six treatments were established: fresh water irrigation (M0T1), recycled water irrigation (M0T2), saline water irrigation (M0T3), magnetized fresh water irrigation (M1T1), magnetized recycled water irrigation (M1T2), and magnetized saline water irrigation (M1T3). The results showed that the magnetization treatment increased the electrical conductivity (EC), power of hydrogen (pH), and dissolved oxygen (DO) of the three water sources compared to the non-magnetized treatment. Furthermore, magnetized irrigation with fresh water, recycled water, and saline water increased the contents of nitrogen (N), potassium (K), calcium (Ca), and magnesium (Mg) in lettuce. It also led to increases in the contents of soluble proteins (by 9.27% to 22.25%), soluble sugars (by 13.45% to 20.50%), and vitamin C (VitC) (by 4.18% to 19.33%) in lettuce. Additionally, it enhanced the above-ground fresh weight of lettuce (by 9.36% to 8.81%) and water productivity (WPc) (by 5.85% to 10.40%), while reducing water consumption. Among these treatments, magnetized fresh water irrigation was the most effective in improving quality, fresh weight, and WPc, followed by magnetized recycled water. Gene expression analysis revealed that differentially expressed genes (DEGs) were primarily enriched in metabolic pathways such as the MAPK signaling pathway—plant, phytohormone signaling, and cysteine and methionine metabolism. In summary, magnetized irrigation significantly enhanced DO levels in irrigation water, along with the fresh weight, quality, and WPc of lettuce, demonstrating its effectiveness as an efficient method for agricultural irrigation.

Marketable and Banned Pesticides in Agriculture: Categorization, Simulation, and Crystallography Review

Pesticides are playing a dominant role in modern cultivation practices to increase agricultural production but are also criticized for environmental depletion and soil and underground water degradation in field applications. An imperative need for greener pesticides has emerged in alignment with new innovations in agrarian and agricultural practices. This study provides a comprehensive review of marketable and banned pesticides that have been applied in past times or are still in use in agriculture. The collected literature production disclosed 35 distinct pesticides that were identified either isolated or in mixtures and residues. These pesticides are primarily applied in agricultural fields, but some of them were also criticized for human implications. Then, these 35 pesticides were grouped into four categories: insecticides (18), herbicides (9), fungicides (6), and acaricides (2). Furthermore, their molecular types, chemical structures, pKa or log Kow values were presented. Based on their chemical structure, the pesticides were also organized into two domains: “marketable simulated” and “banned simulated”, representing 43% and 57% of total pesticides, respectively. The simulations were generated by linking the elemental composition of each pesticide in the corresponding category; therefore, three “marketable simulated” (the acaricides were not marketable representative) and four “banned simulated” were demonstrated. In addition, the calculation of “adjustment factors” (−0.33 up to +0.50) and the “as calculated/marketable (or banned) simulated pesticides” ratios (0.946 up to 1.013) enabled the identification of four clusters of homogeneous characteristics: cluster 1: “Insecticides, Fungicides, marketable”, cluster 2: “Herbicides, marketable”, cluster 3: “Insecticides, Fungicides, banned”, and cluster 4: “Acaricides, Herbicides, banned”. Subsequently, the composition of the elements of C and H enabled the crystallography characterization of only the “marketable” pesticides, not those that are “banned”, with compounds that have been already registered in the “Crystallography Open Database”. Conclusively, implications, challenges, and future research recommendations have been proposed.

Effect of microencapsulated/photoresistant extract of Azadirachta indica A. Juss seeds on Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctudae) and its persistence in semi-field

The development of bioinsecticides as an alternative to synthetic insecticides can benefit the economy of exporting and importing countries, family farming, production of high-quality commodities, food security, quality of life, and the natural environment. This study aimed to evaluate the effect of microencapsulated extracts of Azadirachta indica A. Juss on Spodoptera frugiperda J.E. Smith and their persistence in the plant under semi-field conditions. The fresh seeds of A. indica, collected in Brazil, were separated for the preparation of the microencapsulated dry extract (MDE) according to criteria registered in the patent. Lethal concentrations for S. frugiperda were determined using a dose response curve. The treatments were compared: MDE, control and positive control based on azadirachtin, evaluating mortality and growth inhibition. There was a significant difference in mortality between the treatments tested. The lethal concentrations LC50 and LC95, expressed in azadirachtin for the bioinsecticide, were 194.98 and 630.95 ppm, respectively. The growth inhibition of S. frugiperda was above 98 % in the treatments, except for the control. The MDE presented lethal times LT50 and LT90 lower than the other treatments (lethal time LT50 = 2.79 d; lethal time LT90 = 3.97 d). The MDE of A. indica seeds was effective in controlling S. frugiperda up to the fifth instar and is considered as slightly persistent to persistent depending on environmental conditions and irrigation, based on IOBC/WPRS parameters. The MDE has the potential to be a phytosanitary product approved for use in organic agriculture and with greater effectiveness in controlling S. frugiperda compared to similar pesticides.Abbreviations: BOD, Biochemical Oxygen Demand; CPA, commercial product based on azadirachtin; CLE, concentrated liquid extract; DAS, days after spraying; GI, growth inhibition; LC50, lethal concentration for 50% of the population; LC95, lethal concentration for 95% of the population; LT50, lethal time for 50% of the population; LT90, lethal time for 90% of the population; MDE, microencapsulated dry extract.

CRISPR Technology Acts as a Dual-Purpose Tool in Pig Breeding: Enhancing Both Agricultural Productivity and Biomedical Applications

Pigs have long been integral to human society for their roles in agriculture and medicine. Consequently, there is an urgent need for genetic improvement of pigs to meet human dual needs for medicine and food. In agriculture, gene editing can improve productivity traits, such as growth rate and disease resistance, which could lower farming costs and benefit consumers through enhanced meat quality. In biomedical research, gene-edited pigs offer invaluable resources as disease models and in xenotransplantation, providing organs compatible with human physiology. Currently, with CRISPR technology, especially the CRISPR/Cas9 system emerging as a transformative force in modern genetics, pigs are not only sources of sustenance but also cornerstones of biomedical innovation. This review aims to summarize the applications of CRISPR/Cas9 technology in developing pigs that serve dual roles in agriculture and biomedical applications. Compared to ZFNs and TALENs, the CRISPR/Cas9 system offers several advantages, including higher efficiency, greater specificity, ease of design and implementation, and the capability to target multiple genes simultaneously, significantly streamlining the process of genetic modifications in complex genomes. Therefore, CRISPR technology supports the enhancement of traits beneficial for agricultural productivity and facilitates applications in medicine. Furthermore, we must acknowledge the inherent deficiencies and technical challenges of the CRISPR/Cas9 technology while also anticipating emerging technologies poised to surpass CRISPR/Cas9 as the next milestones in gene editing. We hypothesize that with the continuous advancements in gene editing technologies and successful integration of traits beneficial to both agricultural productivity and medical applications, the goal of developing dual-purpose pigs for both agricultural and medical use can ultimately be achieved.

Mapping Soil Surface Moisture of an Agrophytocenosis via a Neural Network Based on Synchronized Radar and Multispectral Optoelectronic Data of SENTINEL-1,2—Case Study on Test Sites in the Lower Volga Region

In this article, the authors developed a novel method for the moisture mapping of the soil surface of agrophytocenosis using a neural network based on synchronized radar and multispectral optoelectronic data from Sentinel-1,2. The significance of this research lies in its potential to enhance precision farming practices, which are increasingly vital in addressing global agricultural challenges such as water scarcity and the need for sustainable resource management. To verify the developed method, data from two experimental plots were utilized. These plots were located on irrigated soybean crops, with the first plot situated on the right bank (plot No. 1) and the second on the left bank (plot No. 2) of the lower Volga River. Two experimental soil moisture geodatasets were created through measurements and geo-referencing points using the gravimetric method (for plot No. 1) and the proximal sensing method (for plot No. 2) employing the Soil Moisture Sensor ML3-KIT (THETAKIT, Delta). The soil moisture retrieval algorithm was based on the use of a neural network to predict the reflection coefficient of an electro-magnetic wave from the soil surface, followed by inversion into soil moisture using a dielectric model that takes into account the soil texture. The input parameter of the neural network was the ratio of the microwave radar vegetation index (calculated based on Sentinel-1 data) to the index (calculated based on the data of multispectral optoelectronic channels 8 and 11 of Sentinel-2). The retrieved soil moisture values were compared with in situ measurements, showing a determination coefficient of 0.44–0.65 and a standard deviation of 2.4–4.2% for plot No. 1 and similar metrics for plot No. 2. The conducted research laid the groundwork for developing a new technology for remote sensing of soil moisture content in agrophytocenosis, serving as a crucial component of precision farming systems and agroecology. The integration of this technology promotes sustainable agricultural practices by minimizing water consumption while maximizing crop productivity. This aligns with broader environmental goals of conserving natural resources and reducing agricultural runoff. On a larger scale, data derived from such studies can inform policy decisions related to water resource management, guiding regulations that promote efficient water use in agriculture.

Quantitative measures of recent and lifetime agricultural pesticide use are associated with increased pesticide concentrations in house dust

ObjectiveElevated pesticide concentrations have been found in dust from homes with residents who use agricultural pesticides, but few studies have compared these concentrations to quantitative measures of their use. We evaluated household pesticide dust concentrations in relation to quantitative, active ingredient-specific metrics of agricultural pesticide use in the Biomarkers of Exposure and Effect in Agriculture Study. MethodsParticipants provided vacuum dust samples (2013–2018) and information regarding recent (last 12 months) and lifetime pesticide use. Thirty-two pesticide analytes were measured in 295 dust samples from 213 participants; 54 had repeated measurements (median = 96 days between visits). We used mixed-effects quantile regression models to estimate relative differences in pesticide concentrations for recent and lifetime agricultural use (number of days, intensity-weighted days), recent home/garden use (yes/no), and household characteristics. Only household characteristics were examined for dacthal because of no use information. We calculated intraclass correlation coefficients (ICCs) to evaluate temporal variability. We report only descriptive statistics for pesticides with detection rates <25 %. ResultsFor currently used pesticides, quantitative measures of recent agricultural use were associated with significantly increased household pesticide dust concentrations for malathion, metolachlor, acetochlor, cyfluthrin, and atrazine (p-trends < 0.001), but not permethrin. Similarly, quantitative measures of lifetime use were associated with increased concentrations of malathion, metolachlor, carbaryl, diazinon, and atrazine (p-trends < 0.001), but not permethrin, chlorpyrifos, or chlorothalonil. For banned pesticides, ever agricultural use was associated with elevated chlordane and heptachlor concentrations and non-significantly elevated dieldrin concentrations, but not lindane, p,p-DDD, p,p-DDE, or p,p-DDT. Recent home/garden use predicted increased malathion, carbaryl, and cyfluthrin concentrations. ICCs (range = 0.57–0.90) suggested moderate to high correlation over 3–6 months. Detection rates were <25 % for alachlor, butylate, EPTC, metribuzin, simazine, carbofuran, coumaphos, as well as for three banned pesticides (cyanazine, aldrin, endosulfan). ConclusionsHousehold pesticide dust concentrations were strongly associated with the frequency of agricultural pesticide use.

Effect of Biostimulants on Plant Growth and Leaf Functional Compounds of Passiflora Plants

The influence of biostimulant treatments on Passiflora species was assessed by observing changes in the growth and leaf functional compounds of plants exposed to humic acids (HAs) and seaweed extracts (SEs) to determine their optimal application doses. Four different concentrations of HA irrigation (0, 144, 192, and 285 mg·L−1 per pot assigned as HI-1, 2, 3, and 4, respectively), HA spraying (0, 96, 128, and 192 mg·L−1 as HS-1, 2, 3, and 4, respectively), SE irrigation (SI-1∼4 at 0, 1, 1.33, and 2 g·L−1 per pot, respectively), and SE spraying (SS-1∼4 at 0, 0.5, 0.67, and 1 g·L−1, respectively) treatments were applied to ‘Tainung No. 1’ and Passiflora suberosa cultivars every week to study their responses in plant growth traits and metabolites. Results show that leaf dry weight (DW) and root DW of ‘Tainung No. 1’ plants under SS-3 and HI-4 treatments, respectively, were significantly increased compared with controls. Both HI-3 and HS-3 treatments remarkably increased vine length, fresh weight (FW), and DW of leaves and shoots in P. suberosa plants compared with controls. Furthermore, ‘Tainung No. 1’ and P. suberosa plants respectively subjected to HI-2 and SS-4 treatments contributed to developing biostimulant applications for obtaining higher total phenol and flavonoid content per plant. Both Passiflora species are recommended for treatment with SS-4 and SE to maximize orientin and isovitexin content, and for all their beneficial applications and sustainable use in agriculture.

Detection in Orchards of Predominant Azole-Resistant Candida tropicalis Genotype Causing Human Candidemia, Taiwan.

Fluconazole-resistant clade 4 Candida tropicalis causing candidemia in humans has been detected in tropical/subtropical areas, including those in China, Singapore, and Australia. We analyzed 704 individual yeasts isolated from fruits, soil, water, and farmers at 80 orchards in Taiwan. The most common pathogenic yeast species among 251 isolates recovered from farmers were Candida albicans (14.7%) and C. parapsilosis (11.6%). In contrast, C. tropicalis (13.0%), C. palmioleophila (6.6%), and Pichia kudriavzevii (6.0%) were prevalent among 453 environmental isolates. Approximately 18.6% (11/59) of C. tropicalis from the environment were resistant to fluconazole, and 81.8% (9/11) of those belonged to the clade 4 genotype. C. tropicalis susceptibility to fluconazole correlated with susceptibilities to the agricultural azole fungicides, difenoconazole, tebuconazole, and triadimenol. Tandem gene duplications of mutated ERG11 contributed to azole resistance. Agriculture environments are a reservoir for azole-resistant C. tropicalis; discontinuing agricultural use of azoles might reduce emergence of azole-resistant Candida spp. strains in humans.

Modification of chitosan-ethyl formate polymer with zinc oxide nanoparticles and β-CD to minimize the harmful effects of Alternaria early blight on Vicia faba

Derivatives of chitosan-ethyl formate polymers (Chs-EF) show promise as biologically relevant materials. The novelty of this study lies in the innovative use of Chs-EF doped with zinc oxide nanoparticles and beta-cyclodextrin, which significantly enhances the polymers' protective activities against Alternaria early blight disease in Vicia faba by improving both disease resistance and plant health. After doping Chs-EF with zinc oxide nanoparticles (ZnONPs) and inserting it into the beta-cyclodextrin (CD), two products emerged: Chs-EF/ZnONPs and Chs-EF/CD. Using βCD and ZnONPs to modify the Chs-EF polymer improves the optical properties of the generated polymers. Also, the energy gab values of the modified polymers (Chs-EF/ZnONPs and Chs-EF/βCD) were 3.3 and 3.7 eV, respectively, while energy gab value of the Chs-EF polymer was 3.9 eV. In this study, the effects of ZnONPs, chitosan, β-CD, and Chs-EF/ZnONPs on Alternaria solani early blight disease in Vicia faba plants were investigated. The treatments were evaluated based on disease symptoms and a disease index (DI) to assess their ability to protect against Alternaria early blight disease blight. The results show that the modified polymer with ZnONPs and beta-cyclodextrin (β-CD) and the modified polymer with ZnONPs (Chs-EF/ZnO NPs) provided the best protection, with DI values of 25 % and 12.5 %, respectively. Furthermore, it was discovered that the levels of carotenoids, chlorophyll a, and chlorophyll b in the infected plants had dropped by 52.6 %, 69.2 %, and 36.1 %, respectively. Chs-EF/ZnONPs were the most effective treatment, showing significant increases in the contents of chlorophyll a and b in infected plants by 120.8 % and 225.4 %, respectively. The study revealed that Chs-EF/ZnONPs exhibited a 131 % increase in the total phenolic content of plants, peroxidase (POD) activity (110.6 %), and a 347 % increase in polyphenol oxidase (PPO) activity, respectively, compared to healthy plants. Malondialdhyde (MDA) decreased by 50.7 %, 49.7 %, 43.4 %, 36.6 %, 31.7 %, and 7.5 % in response to Chs-EF/ZnONPs, Chs-EF/β-CD, Chs-EF, ZnONPs, Chitosan, and β-CD, respectively. Also, application of Chs-EF/ZnONPs, Chs-EF/β-CD, Chs-EF, ZnONPs, Chitosan, and β-CD reduced the production of H2O2 by 77.5 %, 62.8 %, 62.5 %, 39.6 %, 22 %, and 15.1 %, respectively, compared to infected controls. We recommend considering these substances as promising candidates for agricultural use, as they may effectively serve as control agents against early blight caused by Alternaria solani.

Prediction of environmental suitability for Haematoxylum campechianum: a proposal to promote reforestation in Mexico

Models of potential distribution and ecological niche allow to estimate priority areas for the management of species of interest, as well as to identify variables related to environmental suitability and predict the behavior of species in the face of anthropogenic disturbances. Logwood (Haematoxylum campechianum) is a species that we consider a natural heritage in southeastern Mexico because of its ecological role and the current and historical use of its wood. This species is present in different vegetation types and even dominates in some ecosystems called "tintales", which have been exploited for dye extraction in past centuries. This, combined with changes in land use for livestock, agriculture, and other anthropogenic activities, has affected the Logwood´s distribution and connectivity of its populations. We modeled the potential distribution of H. campechianum and evaluated the feasibility of reforestation with this species in southeastern Mexico. For this purpose, we included 638 occurrence points and used the MaxEnt algorithm for modeling, analyzing different parameters. We used the potential distribution model, accessibility to the areas and water availability to calculate a feasibility index for reforestation. The environmental suitability for the species is more present in southeastern Mexico, conditioned by the aridity index, the maximum temperature of the coldest month and the warmest month. The greatest percentage of areas with high feasibility indexes for reforestation are found in Campeche, apparently a key state for the conservation and management of the species. This study can be the starting point for implementing reforestation programs with H. campechianum and can be used as an example to apply to other species with patrimonial value.