Agricultural Research Institute Research Articles

Seed Production in Jalandhar

A key factor in agricultural resilience, crop diversity, and productivity is seed production—particularly in areas like Jalandhar that must meet changing market demands and climate change. This project explores the complexities of seed production in Jalandhar and how it affects agricultural development with the goal of educating researchers, policymakers, and agricultural stakeholders on how to support sustainable seed production systems. Located in the rich plains of Punjab, Jalandhar has become a major centre for the production of seeds, especially for pulses, wheat, and rice. The region's favorable soil and climate, together with cutting-edge farming techniques, are what propel the expansion of the seed business. With the growing use of hybrid and genetically modified seeds, government programs encouraging agricultural output, and growing farmer awareness of the advantages of high-quality seeds, Jalandhar has a huge market potential for seed production. Nevertheless, in order to fully realize the potential of this industry, stakeholders must work together and implement strategic interventions in response to issues including volatile market pricing, restricted financial availability for small-scale farmers, and environmental sustainability concerns. The sector's growth trajectory is further aided by the existence of well-established agricultural research institutions and government policies that are supportive of the industry, providing growing opportunities in both domestic and foreign markets High-quality seeds improve crop yields while also contributing to overall sustainability and profitability in farming operations. Agro-ecologically-suited certified seeds are essential for reducing the danger of pests, illnesses, and unfavorable weather. Encouraging and supporting sustainable seed production systems in Jalandhar benefits all related departments and financial institutions by promoting agricultural development, economic success, and food security. Keywords : Seed production ,Agricultural development ,Market potential ,Sustainable seed systems ,Government initiatives ,Economic prosperity.

First Report of Wilt Caused by Fusarium nanum (FIESC 25) on Mungbean (Vigna radiata) in Pakistan.

Mungbean, Vigna radia (L.) R. Wilczek, is ranked 2nd next to chickpea (Cicer arietinum) in total cultivation and production in Pakistan. In August of 2022 and 2023, mungbean plants (cv. PRI Mung-2018) were found wilting in a field at the Ayub Agricultural Research Institute, Faisalabad, Pakistan. Wilted leaves turned yellow, died, but remained attached to the stem. Vascular tissue at the base of the stem showed light to dark brown discoloration. Roots were stunted with purplish brown to black discoloration. Symptomatic mungbean plants were collected from fields at five different locations (20 samples/location). Disease incidence was similar among the five fields, ranging from 5 to 10% at each location depending upon type of germplasm and date of sowing. For fungal isolation and morphological identification, symptomatic stem and root tissues were cut into ~5 mm2 pieces with a sterilized blade. Tissues were surface-sterilized for one min in a 0.5% sodium hypochlorite solution, rinsed twice in sterilized water, air dried on sterilized filter paper, and aseptically placed on potato dextrose agar (PDA) containing 0.5 g/L-1 streptomycin sulphate. Plates were incubated for 3-4 days at 25 ± 2°C with a 12-h photoperiod. Single-spore cultures were used for morphological and molecular analyses. Isolates on PDA grew rapidly and produced abundant white aerial mycelium that turned off-white to beige with age. Macroconidia were hyaline, falcate, typically 3-to-6 septate with a pointed apical cell and a foot-shaped basal cell, measuring 24.5-49.5 x 2.7-4.7 μm (n = 40). Globose to obovate chlamydospores measuring 5.8 ± 0.5 μm (n = 40) were produced singly or in chains and were intercalary or terminal and possessed roughened walls. The morphological data indicated the isolates were members of the genus Fusarium (Leslie and Summerell 2006). To obtain a species-level identification, a portion of translation elongation factor 1-α (TEF1), the largest subunit of RNA polymerase (RPB1), and the second largest subunit of RNA polymerase (RPB2) region were PCR amplified and sequenced using EF1/EF2 (O'Donnell et al. 1998), Fa/G2R (Hofstetter et al. 2007), and 5f2/7cr (Liu et al. 1999) primers, respectively. DNA sequences of these genes were deposited in GenBank under accession numbers MW059021, MW059017 and MW059019, respectively. The partial TEF1, RPB1 and RPB2 sequences were queried against the Fusarium MLST database (https://fusarium.mycobank.org/page/Fusarium_identification), using the polyphasic identification tool. The BLASTn search revealed 99.9% identity of the isolate to F. nanum (Xia et al. 2019), formerly FIESC 25 of the F. incarnatum-equiseti species complex (MRC 2610, NRRL 54143; O'Donnell et al. 2018). To confirm pathogenicity, roots of 3-5 leaf stage mungbean seedlings were soaked in a 106 spores ml-1 conidial suspension of the fungus for 15 min and then planted in 10 cm pots containing sterilized soil. Mock-inoculated plants with sterile water served as a negative control. Twenty pots that were used for each inoculated and control treatment were maintained at 25 ± 2°C, 14:8 h photoperiod, and 80% relative humidity in a growth chamber. After 15 days, leaf yellowing, internal browning from the base of stems and root discoloration was observed in all the inoculated plants. The uninoculated negative control plants remained asymptomatic. Fusarium nanum was re-isolated from artificially inoculated plants and identified by colony growth, conidial characteristics on PDA and molecular analyses (TEF1). To our knowledge, this is the first report of wilt caused by F.nanum on mungbean in Pakistan. In Pakistan, mungbean cultivation in irrigated areas has increased in recent years. It has been introduced frequently in citrus orchards, crop rotation of maize and sesame, intercropping with sugarcane and as green manure. However, citrus, maize, sesame and sugarcane are also hosts of Fusarium spp. Therefore, this information warrants sustainable crop protection and may have an impact on further interaction of F. nanum with other wilt pathogens.

Exploring the Genetic Diversity and Agronomic Performance of Okra Varieties Using Variance Analysis

The experiment was conducted at the experimental field of Genetics and Plant Breeding Department of Sher-e-Bangla Agricultural University, Dhaka Bangladesh during March 2017 to July 2017. The experiment was conducted in randomized complete block design with three replications. Twenty eight genotypes of okra were used in this study which was collected from Bangladesh Agricultural Research Institute (BARI), Gazipur and local market. The extent of diversity present between genotypes determines the extent of improvement gained through selection and hybridization. The more divergent the two genotypes are the more will be the probability of improving through selection and hybridization. Mean square of most of the characters studied revealed that genotypes showed highly significant (P< 0.01) differences for all the traits studied. High genotypic and phenotypic coefficient of variation were observed for primary branches (43.91 and 33.64) and fruit yield per plant (37.51 and 32.48). So, divergent genotypes are recommended to use as parents in future hybridization program.

Breeding value of potato hybrid clones from the VIR collection revealed in the environments of Kamchatka Territory

Background. Potato breeding involves planned artificial hybridization between previously selected parental lines. A way to make potato breeding more efficient is to use the diversity of the germplasm and broaden the gene pool of parental lines. Interspecific potato hybrids originated on the elite potato germplasm display a set of valuable traits. The current challenge is to assess the performance of potato hybrids under specific soil and climate conditions to clarify their breeding value.The objective of the study was to evaluate potato hybrids in the environments of Kamchatka Territory according to a set of traits, identify the best parental lines, develop new hybrid combinations, and select promising breeding material.Materials and methods. Eleven potato hybrids from VIR were studied over three years (2020–2022) in the fields of Kamchatka Research Institute of Agriculture. The hybrids were benchmarked against two reference potato cultivars, ‘Fresco’ and ‘Vulkan’, belonging to the early and mid-early maturity groups, respectively. Crosses between the hybrids and 28 potato cultivars were carried out in the greenhouse. The new hybrid material was tested in a preliminary trial nursery for yield and earliness.Results. Hybrid 117-2 was better in yield (899 g/plant) than cvs. ‘Vulkan’ and ‘Fresco’ (700–739 g/plant). Hybrids 94-5, 99-10- 1, 8-1-2004, 8-3-2004, 8-5-2004, 135-3-2005, 135-5-2005, 99-6-6, and 134-2-2006 in most traits matched the potato cultivars, manifesting good berry formation and seed setting when crossed. Out of 140 crosses between 11 hybrids and 28 cultivars, 35 crosses between 10 hybrids and 13 cultivars were successful. Most hybrids were effectively used in crossings as maternal forms.Conclusion. VIR’s hybrids 94-5, 8-1-2004, and 8-5-2004 proved the most promising as source material for the development of new early potatoes under the conditions of Kamchatka Territory.

Physico-chemical, functional, pasting and structural characteristics of two malted maize flours produce under optimal conditions

Maize is a popular gluten-free grain, used in many dishes in Africa and elsewhere. It provides numerous nutrients (especially starch) and contains antinutritional factors (phytates). To reduce these factors and improve the nutritional and physical potential of maize, a number of treatments are required, including malting. This study therefore investigated the physicochemical, functional, structural, and pasting properties of two malted maize varieties (Atp-Y and Coca-sr) produce under optimal conditions. After receiving the two maize varieties from the Agricultural Research Institute for development, the Atp-Y variety was soaked for 30 h at 18.66 °C with 0.37% plant salt, followed by germination at 30 °C for 218.58 h and a ripening period of 40 h, while the Coca-sr variety was soaked for 3.90 h at 20.57 °C with 0.0219% plant salt, followed by germination at 30 °C for 251.55 h and a ripening period of 58.73 h. The flours obtained were subjected to physicochemical, structural, pasting and functional analysis. These analyses showed that the protein, fibre, ash, carbohydrate, starch and energy density content were significantly influenced by the variety. The Atp-Y variety had the highest contents of Ca (855.00 mg/100 g), Na (125.36 mg/100 g) and Cu (0.53 mg/100 g). The availability of Ca, Na and divalent cations was significantly higher in the Atp-Y variety. The Coca-sr variety contributed the most to achieving the protein (40.02 to 73.88%) and lipid (27.30 to 38.08%) requirements of children aged 0 to 59 months. The contents of phytates, cyanides and condensed tannins were similar in both varieties, while oxalates (7.88 mg/100 g), trypsin inhibitors (2.07 mg/100 g) and hydrolyzed tannins (15.02 mg EC/g) were more abundant in the Coca-sr variety. Phenols, flavonoids and saponins ranged between 45.96 and 68.39 mg GAE/g, 32.13 and 39.27 mg CE/g and 0.45 mg/100 g, respectively. No significant differences were found in the physical and functional properties, although the Atp-Y variety had a higher water retention and swelling capacity. Malting variety and conditions had no significant effect on Pasting Time, Setback, Setback ratio and Stability ratio in contrast to Pasting Temperature, Peak viscosity, Holding viscosity, Breakdown and Final viscosity. The Atp-Y grade exhibited the highest pasting parameters when pasting temperature was excluded. Scanning electron microscopy showed that the microscopic structure varied from one variety to another, although the grains had similar sizes. The Microscopic dispersions of grains testifies to the enzymatic activity in the Coca-sr variety, the compact surface layer with protruding grains testifies to the fiber richness of the Atp-Y variety. The grain distribution of the particles showed that more than 50% of the grains in both types were 400 µm in size. X-ray diffraction (XRD) showed that the degradation of the crystalline structure of the starch depended on the variety and the physiological phenomena taking place there. These results clearly show that these flours are well suited for the formulation of foods with high energy and nutritional value. However, they could also be used as sweeteners in panification or as raw materials in the brewing industry.

Evaluation of In Vitro Antimicrobial, Cytotoxic, Thrombolytic, and Antiarthritic Property of Different Parts of Bari Orchid.

Many different herbal extracts have historically been utilized to treat microbe-induced infections, injuries, cancer, thrombosis, and arthritis. The purpose of this study was to determine the antibacterial, cytotoxic, in vitro thrombolytic, and in vitro antiarthritic properties of ethanolic extracts of stem and seed of Bari orchid 1 (BO) plant. This orchid plant was developed by the Bangladesh Agriculture Research Institute (BARI) in Gazipur. Fourteen microbes were employed in the antimicrobial investigation, and samples of orchids were compared to ciprofloxacin as a reference. The BO/seed extract was found to possess more antibacterial activity. The lethality test of brine shrimps was used to assess the LC50 values. The BO/stem extract exhibited a higher cytotoxicity potential, in comparison to the BO/seed extract. Two concentrations (1000 and 100 ppm) and two incubation times (24 hours and 1.5 hours) were used to assess the thrombolytic activity of the extracts. Regarding the thrombolytic effect, the BO/stem extract has demonstrated greater promise. Furthermore, the herbal extract's antiarthritic activity was investigated at four different concentrations, and the results were evaluated in comparison with those of diclofenac sodium. When comparing BO/stem extract to other extracts, the greatest values for protein denaturation were obtained.

Brown manuring in maize (Zea mays): Effects on weed interference and crop productivity

A field experiment was undertaken during rainy (kharif) season 2017 at ICAR-Indian Agricultural Research Institute, New Delhi to study the effects of brown manuring on weeds and maize (Zea mays L.) productivity. Season-long weed infestation (~unweeded control) resulted in the highest reduction (~41%) in grain yield of maize. Among the brown manuring (BM) treatments, growing of 1:1 mixture of Sesbania bispinosa (12.5 kg seed/ha) + Crotalaria juncea L. (12.5 kg seed/ha) supplemented with 2,4-D 0.5 kg/ha applied at 35 DAS, resulted in the highest reduction in weed density (~93%) and dry matter (~94.4%). This BM, in these regards, was comparable with the atrazine + hand weeding (HW) at 35 days after sowing (DAS) and weed-free control treatments. All BM treatments suppressed noxious weed Cyperus rotundus, which was poorly or not controlled by the recommended tank-mixture of atrazine and pendimethalin. Weed-free control resulted in the highest values of maize plant dry-matter (~210.6 g/plant), cob weight (~155.9 g/cob), and grain yield (~6.0 t/ha). The atrazine + HW, Sesbania + Crotalaria (12.5 + 12.5 kg/ha) mixture applied with 2,4-D 0.5 kg/ha at 25 DAS, Sesbania 25 kg/ha with 2,4-D 0.5 kg/ha at 25 DAS, and pendimethalin 0.75 kg/ha + atrazine 0.75 kg/ha treatments were comparable with weed-free control on maize grain yield.

Improving upland rice (Oryza sativa l.) performance through enhanced soil fertility and water conservation methods

The experiment was conducted at Experimental sites of Tanzania Agricultural Research Institute (TARI) – Ukiriguru, Mwanza, Tanzania. The objective was to improve performance of upland rice through enhanced soil fertility and water conservation methods. A split–split plot experiment in a randomized complete block design with three replications and three factors that were (a) upland rice cultivars (WAB 450, NERICA1 and NERICA4) (b) fertilizer types, (Urea 80Kg N/ha, farm yard manure 5 t/ha and control) (c) three water conservation methods (flat cultivation, open ridge and tie ridge were applied). Rice was sown in seven rows at 30 cm inter–and intra–row spacing. Data on soil, weather, crop growth, yield components and grain yield were collected. Rainfall was 651.2 mm during the cropping season which was poorly distributed. Average temperature was 24oC with mean relative humidity of 75%. Soil analysis results showed that total nitrogen was 0.08 %, phosphorus 2.09mgP/Kg and organic carbon 0.58%. The soil calcium was 3.38 cmolc/Kg and potassium was 0.36cmolc/Kg, while zinc was 0.39 mg/Kg. Cultivars had significant effect on yields which were 2856, 2507 and 2140 Kg/ha for WAB 450, NERICA4 and NERICA1 respectively. Fertilizer types also significantly affected grain yield in the order of urea (3368 Kg/ha) > FYM (2723 Kg/ha) and > control (1421 Kg/ha). Moisture conservation methods significantly affected yield in this order: tie ridges (2710 Kg/ha) > open ridge (2398 Kg/ha) > flat cultivation (2394 Kg/ha). Overall, the study results indicated low fertility status in the soil, although it was found to be suitable for upland rice production with further improvement. It is concluded that rice cultivar, WAB 450 had the highest yield potential, while application of Urea at 80 Kg N/ha gave high grain yield, while tie ridges were the best in soil moisture conservation.

Paddy Crop Disease Detection Using Machine Learning

Abstract: India has risen as a significant player in earlier years, the world’s second-largest producer of paddy, yielding approximately 497.7 million metric tons annualy. Sustaining vast paddy fields demand ongoing attention and upkeep. It is critical to recognize the symptoms and understand how this can effectively control the disease. Therefore, inspired by this research paper, a solution is suggested to train machine learning for identify diseases in paddy plants. The system utilizes rea;- time datasets sourced from the Agriculture Research Institute of Lonavala, which are freely accessible. This system can be used for identification purposes four major paddy plant diseases -Leaf Blast, Leaf Scald, Neck Blast and False smut. This Proposed system, first images are pre-processed and then the custom Convolution Neural Network is used to arranging the images. This project work discusses the methodology for disease identifying diseased leaves at an early At this stage, the suggested automated system can assist farmers in mitigating further harm to their crops.

First Report of 'Candidatus Phytoplasma asteris'-related Strain (16SrI group) Associated with Small Leaves, Leaf Yellowing, and Shoot Proliferation of Morning Glory (Ipomoea biflora) in Taiwan.

Ipomoea biflora L., commonly known as morning glory, is an herbaceous vine plant in the Convolvulaceae family and is widespread at low elevations in Taiwan and other East Asian countries. In September 2023, six I. biflora plants exhibiting small leaves, leaf yellowing, and shoot proliferation were observed in a vacant lot in Taiwan Agricultural Research Institute (TARI), Wufeng District, Taichung, Taiwan, representing 100% disease incidence in the area. All the symptomatic morning glory climbed onto Murraya paniculata L. (common jasmine orange) which however showed no similar symptoms. The total DNA (two samples for each plant) from leaf tissues of three symptomatic morning glory plants, two asymptomatic morning glory plants, and one asymptomatic common jasmine orange was isolated by the CTAB method (Fulton et al. 1995) and used for PCR with the universal primers, P1 (Deng and Hiruki 1991)/P7 (Schneider et al. 1995), to amplify a fragment containing partial 16S rDNA. Expected 1.8-kb bands were amplified from DNA extracted from all symptomatic plants, whereas no PCR product was detected from that of the asymptomatic I.biflora and M. paniculata plants. Six PCR products were cloned and sequenced in the Biotechnology Center DNA-sequencing facility at National Chung Hsing University, and one representative sequence was selected and deposited in GenBank. BLAST analysis revealed that the obtained 16S rDNA sequence (PP230905) shared 99.92% identity with the following phytoplasma strains: rapeseed phyllody phytoplasma (CP055264), plumbago auriculata leaf yellowing phytoplasma (MN239503), and aster yellows phytoplasma (MK992774), which all belong to the 16SrI subgroup. The query 16S rDNA sequence shares 99.84% identity with that of the 'Candidatus Phytoplasma asteris' reference strain (M30790), suggesting that the phytoplasma is a 'Ca. Phytoplasma asteris'-related strain. A virtual restriction fragment length polymorphism (RFLP) analysis was conducted using iPhyClassifier tool (Zhao et al. 2009), and the pattern derived from the 16S rDNA fragment of the I. biflora phytoplasma was identical (similarity coefficient 1.00) to the reference pattern of 16SrI, subgroup B (onion yellows phytoplasma OY-M; AP006628). Six total DNA samples from symptomatic plants were used as templates to amplify 842 bp secA sequences with SecAfor1 and SecArev3 primers (Hodgetts et al. 2008), and one representative sequence was deposited in GenBank. The partial secA sequence (PP263636) showed 98.22% identity with that of Trema levigatum witches'-broom phytoplasma (MW032212) that also belongs to the 16SrI group (Wan et al. 2021). Phylogenetic analysis of both 16S rDNA and secA confirmed I. biflora phytoplasma as 16SrI, subgroup B. Taken together, we concluded that the morning glory phytoplasma in this study was a 'Ca. Phytoplasma asteris'-related strain belonging to the 16SrI group. To the best of our knowledge, this is the first report of a phytoplasma-infected I. biflora in Taiwan, suggesting morning glory as a new natural host of 16SrI phytoplasmas, alongside other plants like roselle and citrus (Tseng et al. 2014; Feng et al. 2015).

Morphological, phenotypic and nutritional characterization of exotic and local tomato germplasm under tunnel

Tomato (Solanum lycopersicum L.) is one of the most important and widely cultivated fruit. By characterizing local germplasm, exclusive breeding material is successfully identified and improved varieties could be created. The research conducted between November 2020 and 2021 at the Ayub Agricultural Research Institute (AARI) in Pakistan used developmental, vegetative and fruit traits to individualize sixty tomato inbred lines for 17 morphological traits. The study showed that 6 qualitative and 11 quantitative morphological traits exhibited a wide range of variation, while 8 traits showed a high coefficient of variation among genotypes according to biplot principal component analysis (PCA). According to biplot PCA, the first component contributed 17.6% of the variation, while the second component contributed 13.0%. Together, the first and second components explained 30.6% of the variation. A correlation analysis was performed to determine the relationship between genotypic traits and phenotypic traits. A very positive correlation was observed between inter-nodal distance, flower per cluster and immature fruit color. The morphological data for firmness, pericarp thickness, fruit width, TSS, inter-nodal distance, number of flowers and fruits per cluster showed specific characteristics that can be used to distinguish the genotypes. The highest value of lycopene of 21.2 mg kg−1 fresh weight was observed among the genotypes. We conclude the results of this study, presenting the crop quality and yield characteristics that could be used in future plant breeding and genetic enhancement of tomato germplasm for improved food security. Based on yield characteristics, Nyagous, Korneseivje and Earliana were selected as the best and most promising genotypes.

Impact of different decontamination methods on the reduction of spiromesifen residue in chilli fruits

Chilli is an indispensable food item in the daily life of humans but it is affected by many insects, so various pesticides, including spiromesifen, are applied to chilli crops to protect this crop from insect infestation. However, the use of pesticides poses environmental and health issues. These issues have raised the demand for pesticide-free chillies among consumers. The primary aim of this study was to assess the efficacy of various decontamination methods in removing spiromesifen residues from chilli fruits. A randomized block design was employed to conduct a supervised field experiment at the Rajasthan Agricultural Research Institute in Durgapura, Jaipur, India. The samples of chillies treated with pesticides are subjected to seven different homemade techniques. The samples were extracted using the QuEChERS method, known for its efficiency, affordability, simplicity, robustness, and safety. The analysis of spiromesifen residues was conducted using gas chromatography (GC) equipped with an electron capture detector (ECD), and the results were verified using gas chromatography-mass spectrometry (GC-MS). Out of several decontamination methods, the lukewarm water treatment was more effective than any other decontamination method, which led to the highest elimination of spiromesifen residue, whereas rinsing with tap water eliminates the least amount of spiromesifen residue. So, the lukewarm water treatment is a safe, cost-effective, and eco-friendly approach to remove spiromesifen residues from Chilli.

Influence of deficit irrigation and nano NK application on root dynamics, physiology and WUE of transplanted rice (Oryza sativa)

A field experiment was conducted during the rainy (kharif) (June–September, 2022) and winter (rabi) (December 2022–March, 2023) seasons at Agricultural College and Research Institute (Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu), Madurai, Tamil Nadu to study the effect of irrigation methods and nutrient management strategies on the growth of roots, physiological aspects, yield and water use efficiency of transplanted rice (Oryza sativa L.). Experiment was conducted in a split-plot design comprised of 3 irrigation methods, viz. Irrigation at 15 cm depletion of water level in field water tube (M1); Alternate wetting and drying (M2); Conventional flooding (M3) in main plot and 7 nutrient management strategies, viz. Application of 50% N + nano urea as foliar spray (S1); 75% N + nano urea as foliar spray (S2); 50% K + nano potash as foliar spray (S3); 75% K + nano potash as foliar spray (S4); 50 and 75% N and K + nano urea and potash as foliar spray (S5); 100% RDF (S6); and absolute control (S7) in sub plot, replicated thrice. Results indicated that under M1S2, root characteristics like length, volume and dry weight were notably higher. Physiological attributes such as stomatal conductance, transpiration and photosynthetic rate recorded high with M2S5. However, the maximum yield and water use efficiency were observed with M2S5. In conclusion, the treatment combination M2S5 is considered as practical recommendation for farmers in the Periyar Vaigai command area, ensuring optimal yield while economically utilizing water and fertilizers.

Impact of diverse tillage and nitrogen management on growth and yield of conservation agriculture-based wheat (Triticum aestivum)

A study was carried out during the winter (rabi) seasons of 2020–21 and 2021–22 at ICAR-Indian Agricultural Research Institute, New Delhi to assess the effects of diverse tillage and nitrogen management scenarios on growth, development, and yield of a conservation agriculture (CA)-based wheat (Triticum aestivum L.) crop grown in sequence with maize (Zea mays L.). Experiment was conducted in split plot design (SPD) comprised of 3 different tillage practices in main plots [Conventional tillage + residue (CT); Zero tillage + residue (ZT); and Permanent beds + residue (PB)] and 5 nitrogen (N) options in sub plots [Control (zero nitrogen); Recommended dose of N-RDN @150 kg N/ha (50 kg N/ha Basal + 2-equal splits at 37 days after sowing (DAS) and 84 DAS); Green Seeker (GS) based application of N @148 kg N/ha (GS); Urea super granules applied as basal @75 kg N/ha + GS based N application (USG); and Slow release fertilizer as 100% basal application @150 kg N/ha (SRF)] with 3-replications. The findings revealed that in both the seasons, both tillage and nitrogen management approaches significantly affected wheat growth, yield characteristics, and overall yield, whereas the time to anthesis and physiological maturity, and test weight remained unaffected. Within the spectrum of tillage practices, leaf area index (LAI) and yield attributes exhibited the trend PB>ZT>CT. PB recorded the highest grain yield (5159 kg/ha), followed by ZT (4916 kg/ha) and the lowest grain yield was observed with CT (4578 kg/ha). The wheat grain yields were 12.7% and 7.4% higher in PB and ZT, respectively, over to CT. Among nitrogen management options, the grain yield exhibited the pattern USG>N150>SRF>GS>N0. This study emphasizes that adopting conservation agriculture (CA) practices, particularly CA-based permanent beds using urea super granules (USG) for nitrogen management can improve wheat growth and yield.

Pre-storage melatonin treatment maintains cell membrane integrity, reduces fruit browning and decay incidence in guava (Psidium guajava)

An experiments were conducted during 2021–22 at ICAR-Indian Agricultural Research Institute, New Delhi to study the impact of melatonin (MT) on key characteristics of guava (Psidium guajava L. cv. Barafkhana). Guava fruits were treated with different MT concentrations (200, 400, 600 and 800 μM) and stored at 10±1°C with 85–90% RH. The research revealed that treating with 600 μM MT notably decreased polyphenoloxidase activity by 57%, leading to delayed peel browning and also suppressed polygalacturonase (PG) activity by approximately 45% and pectine methylesterase activity by about 73%, resulting in higher firmness (5.33 N). Following a 20-days period of cold storage, significant reductions in electrolyte leakage, hydrogen peroxide accumulation and lipoxygenase activity were observed, thereby preserving cell membrane integrity. Additionally, MT stimulated the antioxidant defense system, boosted proline content accumulation, and enhanced phenylalanine ammonia-lyase activity. Together, these effects contributed to enhanced resistance against postharvest fungal decay (8.75%). In conclusion, MT at a concentration of 600 μM proves to be an effective postharvest treatment for maintaining texture, reducing fruit decay and extending the shelf-life of guava during cold storage.

Determination of insecticide resistance in cotton whitefly in north India

The whitefly, Bemisia tabaci (Gennadius) considered as most destructive pest, poses a significant threat to various crop species globally has developed resistance due to the indiscriminate use of synthetic chemicals. A study was carried out in 2018 at ICAR-Indian Agricultural Research Institute, New Delhi to determine insecticide resistance, in five Asia II 1 populations of B. tabaci from different cotton (Gossypium hirsutum L.) growing regions of north India. The susceptibility of the populations to different insecticide classes, viz. synthetic pyrethroid (cypermethrin); neonicotinoids (imidacloprid and thiamethoxam); thiourea (diafenthiuron) and diamide (cyantraniliprole) were assessed. Results revealed substantial heterogeneity in the responses of these populations to the insecticides. Sriganganagar and Bathinda populations exhibited moderate resistance to cypermethrin, imidacloprid, and thiamethoxam. Low level of resistance was observed in Bathinda and Sriganganagar populations against diafenthiuron. All populations were highly susceptible to cyantraniliprole. No cross-resistance was observed between cyantraniliprole and other insecticides, suggesting its potential as an alternative for managing insecticide resistance. High levels of detoxification enzymes (esterase, cytochrome P450 monooxygenase, and glutathione-S-transferase) in Sriganganagar and Bathinda populations indicated a positive correlation between insecticide resistance and detoxifying enzymes. These findings offer valuable insight for implementing insecticide rotation strategies to combat B. tabaci resistance in India.

Assessment of fungal and bacterial bioagents against Bipolaris sorokiniana inciting spot blotch on barley (Hordeum vulgare)

In India, spot blotch disease caused by Bipolaris sorokiniana is one of the major constraints in barley (Hordeum vulgare L.) production. The present study was carried out during 2022–23 at ICAR-Indian Agriculture Research Institute, New Delhi to identify promising biocontrol agents, which can act as eco-friendly alternatives to chemicals against Bipolaris sorokiniana inciting spot blotch on barley (Hordeum vulgare L.). In this study, 18 isolates of Trichoderma asperellum, 15 isolates of Trichoderma harzianum and 5 bacterial species were tested against B. sorokiniana under in vitro and in planta conditions. In the dual culture assays, Trichoderma asperellum 8686 and Trichoderma asperellum 8687 showed significantly highest per cent disease inhibition of 71.73% and 71.37% respectively. Among bacterial strains, Pseudomonas fluorescens and Bacillus amyloliquefaciens showed significantly good per cent disease inhibition of 64.09% and 57.09% respectively. B. subtilis and Pantoea spp. did not show any pathogen inhibition. In addition, the superior bioagents were also screened out. In the studies on in planta assays, Bacillus amyloliquefaciens (per cent disease control-55.19%) was found most effective for seed treatment against B. sorokiniana. Post-inoculation with biocontrol agents revealed that Bacillus amyloliquefaciens was at par with Trichoderma asperellum and Pseudomonas fluorescens against B. sorokiniana. Additionally, a combination of seed treatment, pre and post-inoculation treatment of biocontrol agents revealed that Bacillus amyloliquefaciens and Trichoderma asperellum 8686 were at par with Trichoderma harzianum (Pusa Th3) against B. sorokiniana. Overall, Bacillus amyloliquefaciens was more effective and consistent to manage spot blotch disease.

Ex-ante priority setting in crop breeding: Towards inclusive innovation for impact

CONTEXTCrop breeding teams should ideally work a-priori to rank research priorities and varietal product concepts to make decisions considering social, environmental, production and market conditions that an improved crop variety will be entering upon release. Frequently, this research prioritization is guided by a cost-benefit optimization framing, which leaves the potential derived by alternative approaches, such as inclusive innovation, unexplored. OBJECTIVEIn this study, we apply PEEP (Participatory Ex-ante framework for Plant breeding), a new methodological framework for ex-ante participatory priority setting for plant breeding programs. This framework explores how, given a set of hypothetical crop varieties (varietal product concepts) it is possible to assess which will have the highest potential to deliver research impacts. PEEP is designed to support crop breeders to understand the impact potential of a new varietal product concept, and strategically make decisions based on this. METHODSPEEP ranks varietal product concepts across five impact domains: breeding, processing, marketing, climate, and cross-cutting themes. This paper reports on how an interdisciplinary breeding team from the Institute of Environment and Agricultural Research (INERA) in Burkina Faso working on cowpea (Vigna unguiculata [L.] Walp) applied PEEP and discusses the results from this application. We chose cowpea as a crop focus, based on prior research that showed that gender and social differences shape roles, responsibilities, and benefits from its cultivation in Burkina Faso. We engaged 650 stakeholders using tools and methods (choice experiment, surveys and focus group discussions) tailored to collect information according to their spheres of knowledge. RESULTS AND CONCLUSIONSWe present a reflection on the application process, results, and feedback from the INERA cowpea breeding team on these. We show that breeders and farmers ranked the potential impact of the varietal product concepts differently. We also show that differences in evaluation of impact from varietal product concepts vary based on market orientation, agroecological zones, farming systems and gender. SIGNIFICANCEWe demonstrate that PEEP contributes to inclusive agricultural innovation as a feasible method to consider the knowledge and preferences of diverse stakeholders. We show that it is informative to include diverse groups when conceptualizing new varieties before investing in varietal development. Making such research prioritization decisions early on enables breeding programs to avoid “missing the mark” on assumed impacts on smallholder women and men farmers and increases their visibility and voice in these upstream decisions.

Design and Development of an Intuitive Android Application for Smart Farming

Farmers face challenges in adapting to environmental changes, yet with decreasing implementation costs and a growing accessibility to technical expertise, progress is evident. This research introduces KisanVishwa, an Android app designed for smart farming. KisanVishwa optimizes precision agriculture systems, enabling farmers to monitor their fields in real time and address agricultural constraints. Tailored to meet the specific needs of today's farmers, the app provides user-friendly tools and the latest information on farm equipment, fertilizers, chemicals, technological advancements, and local distributors and retailers. By integrating KisanVishwa into their operations, farmers can overcome challenges associated with the adoption and maintenance of precision agriculture. KisanVishwa adopts a user-centric design for compatibility across diverse Android devices, integrating monitoring tools and collaborating with agricultural research institutions. Empowering farmers, the app addresses contemporary challenges, fostering informed decisions and efficiency. With its user-friendly interface, KisanVishwa promotes the adoption of precision agriculture, essential resources, and sustainable practices in the agricultural sector. Convincing traditional farmers to adopt advanced technologies may pose challenges. Functionality may depend on reliable internet, posing challenges in areas with limited connectivity.

Planting and Harvesting Mechanization on Productivity, Energy Efficacy and Profitability of Potato Production

Potato occupied the first position among all the vegetables in respect of area and total production in Bangladesh. Effective and efficient use of energy are necessary for an improved agricultural production. Therefore, this research was undertaken for potato production to assess productivity, quantify energy flow and financial profitability of different production systems. The experimented four systems were manual planting and manual harvesting, machine planting and manual harvesting, manual planting and machine harvesting and machine planting and machine harvesting, respectively. The experiment was conducted at Farm Machinery and Postharvest Process (FMP) Engineering Division of Bangladesh Agricultural Research Institute (BARI) during 2017-18. Yield of potato including yield contributing characters for different treatments was not significantly varied. Direct energy was the highest in mechanical planting and harvesting system (25%) and the lower manual planting and harvesting system (18%). Indirect energy shared 82% in manual planting and harvesting system, 78% in machine planting and manual harvesting system, 80% in manual planting and mechanical harvesting system, and 75% in machine planting and machine harvesting system, respectively. The largest source of indirect energy consumption was from fertilizer (22-27% of the total energy). The higher specific energy of 1.93 MJ/kg was found in manually planted and mechanized harvesting system. The higher product value was from manual planting and mechanical harvesting system. The highest BCR (2.25) was found from machine planted and machine harvested followed by manually planted and mechanically harvested plot (2.16), machine planted and manually harvested plot (1.93) and manual planting and harvesting plot (1.91). Thus, mechanically planted and harvested system can be recommended for potato production since it can reduce 52% labour requirement than manual method and can give higher BCR among the tested production methods.