Phosphorus (P) is considered as an essential element in crop production. Besides, the study of P from the elemental and bio sources impacts on productivity and quality of orange trees could emphasize its utilization importance. This study aimed to evaluate the chemical implication of three treatments of P fertilization (Triple phosphate (TP; 620 and 900 g/tree/year), phosphate rock (PR; 1820 and 2742 g/tree/year) and 1:1 TP:PR on Valencia orange trees (Citrus sinensis L. Osbeckunder). Where, all these treatments were equipped with bio-phosphorus fertilizer (phosphorin) (0 and 5 g/tree/year added twice in the year, Feb. and Jun.). The measurements included leaves nitrogen (N), phosphorus (P) and Potassium (K), number of fruits (NoF), juice weight/volume, total soluble solids (T.S.S.), vitamin C (V.C), and acidity. The results showed that the treatment (50% TP +50% PR at 450+1371g/tree/year + phosphourin at 5g/tree/year) gave the highest values for all characters i.e. N % in tree leaves 2.61 and 2.69%, P% in tree leaves 0.16 and 0.17%, K in tree leaves 1.79 and 1.86%, number of fruits/tree 412.0 and 420.0, fruit weight 267.0 and 280.0g, fruit weight/tree 110.0 and 117.60kg, fruit size 260.41 and 272.37cm3, juice weight 114.1 and 126.15g, fruit juice 44.33 and 44.99%, juice volume 110.15 and 114.90cm3, peel thickness 0.58 and 0.60cm, T.S.S. 12.14 and 12.21%, T.S.S. / Acid ratio 9.07 and 9.41, and V.C 70.15 and 72.44 (mg/100 ml) as well as the lowest total acidity 0.93 and 0.91%, under both the 1st season and the 2nd one, respectively. In conclusions, as the application of bio-fertilization has improved the quality charcatistics over seasons. Thus, this study opens the way towards the advanced application of bio-P element to understand the biogeochemical cycles, dynamics and function of natural ecosystems elements in agricultural and food security program.