Weeds significantly impact paddy yields, and herbicides offer a cost-effective, rapid, and efficient solution compared to manual weeding, ensuring agricultural productivity. Tripyrasulfone, a novel 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor developed by Qingdao Kingagroot Chemicals Co., Ltd., has demonstrated high efficacy when applied post-emergence, causing characteristic foliar bleaching in susceptible weed species, distinct from conventional acetolactate synthase, acetyl-CoA carboxylase, and synthetic auxin herbicides. This study investigates the impact of tripyrasulfone on the activity of key soil enzymes (urease (UE), acid phosphatase (ACP), sucrase (SC), catalase (CAT), and dehydrogenase (DHA)) in paddy soils from Jilin Province and Shandong Province. Different doses of tripyrasulfone (0.1, 1.0, and 2.5 mg kg−1) were applied, and the enzymatic activities were measured. Results indicated that tripyrasulfone initially inhibited UE and ACP activities before activating them. On the 20th day after treatment, UE activity had returned to control levels, whereas ACP activity remained significantly higher, showing long-lasting activation. SC and CAT activities were inhibited but gradually recovered to control levels. Furthermore, DHA activity was activated with a sustained effect, remaining significantly higher than the control group even 20 days after treatment. Overall, the impact of tripyrasulfone on soil enzyme activities diminished over time, suggesting that tripyrasulfone posed minimal long-term ecological risk to soil health.