Agricultural Experiment Station Research Articles

The productivity of selected soybean cultivars grown using various cultivation methods

Soybean is an important legume crop globally due to its rich protein, oil content, and functional components. The purpose of this study was to evaluate the yield of selected soybean cultivars depending on cultivation methods. The three-year field experiment, conducted from 2018 to 2020 at the Agricultural Experimental Station in Kępa-Puławy, Osiny farm (Institute of Soil Science and Plant Cultivation – State Research Institute in Puławy), investigated these variations. The first experimental factor was the soil cultivation method: A – conventional tillage, B – reduced tillage, and C – strip tillage. The second variable was soybean cultivar: ‘Aldana’ and ‘Merlin’. The soybean cultivars were selected for their differing maturity rates: ‘Aldana’ (000) is an early cultivar, while ‘Merlin’ (000++) semi-late cultivar. The field experiment utilised a split-plot design on Luvisol soil with sandy loam texture, belonging to a good rye complex, class IIIb–IVa, and was replicated four times. The study showed that the productivity (seed and protein yield) of the ‘Merlin’ cultivar grown in the central-eastern part of Poland was approximately 8% higher than that of the ‘Aldana’ cultivar. The cultivation method had a relatively minor influence on soybean yield, the content of selected nutrients, morphological features, and elements of the yield structure. The soil in strip-tillage method was more compact than the soil cultivated with a plough. After harvesting soybeans at a depth of 30, and 40 cm, the compactness of soil in strip-tillage or with reduced tillage was much lower than in spring, highlighting a positive effect of soybean cultivation on loosening the arable layer.

Registration of ‘MN‐Rothsay’ spring wheat with high grain yield and lodging resistance

Abstract‘MN‐Rothsay’ (Reg. No. CV‐1208, PI 702731) hard red spring wheat (Triticum aestivum) was released by the University of Minnesota Agricultural Experiment Station in 2022 because of its high grain yield and lodging resistance. MN‐Rothsay is a medium‐late maturity cultivar with below average plant height, containing the semi‐dwarfing alleles Rht‐B1b and Rht24b. The lodging resistance of MN‐Rothsay is similar to ‘Linkert’, a 2013 release that was the most widely grown cultivar in Minnesota from 2016 to 2020, largely due to its superior lodging resistance. MN‐Rothsay has moderate resistance to Fusarium head blight (caused primarily by Fusarium graminearum Schwabe), good resistance to prevalent races of pathogens causing leaf rust (Puccinia triticina Eriks.) and stem rust (P. graminis Pers.: Pers. f. sp. tritici Eriks. & E. Henn.), and is well‐adapted to the north central United States.

Nutrient Requirement of Advanced Olitorius Breeding Line O-0512-6-2 for Maximum Growth and Yield

The research work was conducted at Jute Agriculture Experimental Station (JAES) Manikganj and Jute Agriculture Regional Station (JARS) Faridpur of Bangladesh. The present study aimed at determining the nutritional requirement for optimum growth and yield of an advanced olitorius breeding line of O-0512-6-2 in Bangladesh. The experiment was laid out in RCBD with 10 treatments having three replications during the year 2019, 2020 and 2021. The results indicated significant effect on yield and yield contributing characters over control with different fertilizer (NPKS) levels of advanced olitorius breeding line of O-0512-6-2. The highest number of plant height, base diameter, green weight with leaves and without leaves was observed by the treatment T3 (N100P10K25S20) which is statistically significant. The highest fiber yield (2.75 t ha−1) and stick yield (6.50 t ha−1) at Manikganj and highest fiber yield (2.58 t ha−1) and stick yield (6.09 t ha–1) at Faridpur was also obtained by the combined dose of N100P10K25S20 (T3) kg ha−1. Therefore, this combination N100P10K25S20 Kg ha−1 seemed to be optimum for maximum growth and yield of advanced olitorius breeding line of O-0512-6-2. Bangladesh Agron. J. 2024, 26(2): 1-6

Determination of Appropriate Sowing Date on Fibre Yield and Yield Attributes of Jute (Corchorus olitorius L.) var. BJRI Tossa Pat-8 (Robi-1)

The experiment was conducted at Jute Agriculture Experimental Station (JAES), Manikganj; JRRS, Rangpur and JRSS, Jashore to determine the optimum sowing date of jute var. BJRI Tossa Pat-8. The experiment was laid-out in RCBD with three replications and variety O-9897 used as control. Crops were sown on five different dates i.e., 20 March, 30 March, 10 April, 20 April and 30 April regarded as treatment. Crops were attained recommended cultural practices and harvested at 120 days after sowing. The results showed that BJRI Tossa Pat-8 (Robi-1) sown on 10 April to 20 April gave significantly higher fibre and stick yields at Manikganj (3.37 t ha−1 and 6.33 t ha−1 respectively); Rangpur (3.11 t ha−1 and 5.22 t ha−1, respectively) and Jashore (3.96 t ha−1 and 5.57 t ha−1, respectively) with an average 14% higher than control. Bangladesh Agron. J. 2024, 26(2): 27-33

First Report of Strawberry Anthracnose Crown Rot Caused by Colletotrichum siamense in New England.

In the summer of 2023, the Connecticut Agricultural Experiment Station was contacted by a farm in southern Connecticut due to reports of strawberry (Fragaria × ananassa) plants showing signs of severe wilting and crown rot across multiple fields, covering ~20 hectares. Cut crowns from diseased plants had marbled red and white lesions typically associated with anthracnose crown rot (ACR). Symptomatic plants were collected from five June-bearing cultivars (cvs. AC Valley Sunset, Lyla, Dickens, and Allstar) spanning four non-adjacent fields with incidence ranging from 5-90% and severity ranging mild wilting in low incidence fields to severe wilting/mortality in high incidence fields. Internal tissue from diseased crowns was surface sterilized in 0.6% NaOCL for 3 minutes, rinsed with sterile water, and plated on potato dextrose agar. After one-week, hyphal tips of fungi were transferred to fresh plates which formed dense mycelial mats of fluffy, greyish-white hyphae. Orange spore masses formed near the center of the colonies, each of which contained numerous cylindrical and fusiform straight conidia, matching spores within the genus Colletotrichum (De Silva et al. 2019). Average conidia (n=192) length was 15.7 ± 1.6 µm and width was 5.4 ± 0.7 µm. Fungi matching this morphology were isolated from 83% of the collected symptomatic crowns and hyphae were collected from two isolates, CT5-1 and CT23-1, for DNA extraction using the GeneJET Plant Genomic DNA Purification Kit. PCR was performed using primers targeting actin (ACT), calmodulin (CAL), β-tubulin (TUB2), GAPDH (gpdA), and ITS, followed by Sanger sequencing, which yielded identical sequences for both isolates (CT5-1 Accessions numbers: PP002078-81, OR999066)(Carbone and Kohn 1999; Hassan et al. 2018; Templeton et al. 1992). These were combined with sequences from fourteen Colletotrichum genomes, all of which were aligned, trimmed, and concatenated using Mega11 (Tamura, Stecher, and Kumar 2021). Model selection was conducted using IQ-TREE and selected parameters were used to generate maximum-likelihood trees from all five loci individually and the concatenated sequence, all of which placed the isolates in a high confidence cluster with Colletotrichum siamense (Nguyen et al. 2015). To confirm the pathogenicity of the pathogen, strawberry plants (cv. Jewel) (n=5) five weeks after bare root transplant were infected. The base of each crown was penetrated 5 mm deep with a sterile 20 µL pipette tip and then inoculated with 10 µL of spores at a concentration of 106 spores/mL. Control plants (n=5) were inoculated with 10 µL of sterile water. Plants were maintained at 30°C day (16-hour)/20°C night (8-hour) in a growth chamber and assessed after 14-days. Four of the five inoculated plants had visible wilt symptoms and bisected crowns revealed the marbled red and white lesions typifying ACR. Control plants had no clear wilting and bisected crowns were visibly healthy. C. siamense re-isolated from infected tissue presented with identical hyphal /spore morphology and ITS/Tub2 were re-amplified and sequenced, yielding identical sequences to CT5-1. Plant inoculations with the same variety were repeated, yielding identical symptom development and crown lesions. C. siamense has been a dominant source of ACR throughout the southeastern US but has not previously been a major problem in the Northeast. Given the extent of the field infection, it is likely that these isolates can survive the colder winter temperatures of New England, but further experimentation is necessary to determine the extent of the pathogen's winter hardiness.

Scientific achievements in Siberian agriculture and their development

A historical review of the formation and development of scientific farming in Siberia is given. The main achievements of the leading research institutes of the region are highlighted. The contribution of Siberian scientists to the development of scientific farming, including its constituent directions, is shown. It presents almost 100 years of development of research on agriculture - from the Omsk experimental farm (XIX century), agricultural experimental stations - to modern federal scientific centers. The evolution of farming from individual crop cultivation techniques to the formation of zonal and then adaptive-landscape farming systems and modern agro-technologies is demonstrated. Examples of completed scientific developments on farming, ready for implementation in production, the number of which runs into thousands, are given. Implementation of knowledge-intensive agro-technologies guarantees grain production in Siberia up to 25-30 million tons, sustainable development of farming, stable food supply and high return on non-renewable resources. The evolution of plant protection from individual methods against the most dangerous pests to the formation of integrated systems that take into account the phytosanitary state of agrocenoses and agro-ecological conditions of agricultural crops cultivation is shown. The most important techniques to optimize the relationship between the crop and pests are the selection of plant species and varieties resistant to their effects, optimization of mineral nutrition of plants and sowing dates. At present, the development of scientific farming is associated with the design of adaptive-landscape farming systems, selection and support of agro-technologies, rational use of land resources, improvement of approaches to the conservation and reproduction of soil fertility, soil treatment systems, fertilizers and integrated plant protection, formation of adaptive crop rotations, research of technological and biological processes in the cultivation of crops, use of GIS-technologies, remote sensing, and the use of remote sensing.

First Report of Neopestalotiopsis spp. Causing Leaf Spot and Petiole Blight on Strawberry in New England.

In October 2023, a Connecticut grower contacted The Connecticut Agricultural Experiment Station about a field of strawberry plants (Fragaria × ananassa) (cv. Ruby June) showing symptoms of severe leaf spotting and visual wilting. Upon visiting the field, leaves had lesions with a diffuse black halo and a light brown center and wilting symptoms, which appeared driven by petiole lesions and presented as dark brown stripes with a reddish-purple halo. Symptoms were observed on 80 to 90% of plants within the block, nearly all of which (>90%) presented with both leaf spots and severe wilting. Diseased tissue was collected from 20 leaves and 25 petioles, sterilized in 0.6% NaOCL, and plated on potato dextrose agar. After hyphal tipping a morphologically identical fungus was isolated from 70% of leaves and 88% of petioles, which formed a dense white mycelial mat with moderate aerial mycelium and conidiomata that exuded dark brown conidial masses. The underside of the mycelial mat was yellowish. Conidia were fusoid, ellipsoid, straight to slightly curved, 4-septate with a single basal appendage and 2-5 apical, matching the description of species within the genus Neopestalotiopsis (Maharachchikumbura et al. 2014). The average conidia (n=74) length, not including appendages, was 29.9 ± 2.1 µm and the average width, at the widest point, was 7.5 ± 0.7 µm. Aerial hyphae were collected from two isolates, CT58-1 and CT62-2, and DNA was extracted for further molecular characterization. PCR was performed with primers targeting actin (ACT), β-tubulin (TUB2), and ITS prior to amplicon sequencing (Carbone and Kohn 1999; Hassan et al. 2018). Sequences were queried against the NCBI whole genome shotgun database, and aligned sequences from 13 species (including Neopestalotiopsis, Pestalotiopsis, and Pseudopestalotiopsis) were collected for each locus. Sequences were aligned, trimmed, and concatenated using Mega11, and IQ-TREE was employed for model selection (Nguyen et al. 2015; Tamura et al. 2021). A maximum-likelihood tree placed the isolates in a high-confidence cluster with Neopestalotiopsis rosae, confirming this placement of these isolates within the genus (CT58-1 Accession #: PP715979-89; PP707735). To confirm pathogenicity, CT58-1 was grown on autoclaved strawberry leaves to induce sporulation, and a suspension of 105 spores/ml was made. Five milliliters of this spore suspension was sprayed on six 6-week-old strawberries (cv. Jewel), and water was sprayed on the same number of control plants. Plants were at 100% humidity for two days and then kept in the greenhouse for 3 weeks to observe symptoms. Inoculated plants presented with identical leaf spot and petiole lesions to field samples and no visual symptoms were observed on control plants. New isolations were made from infected petioles, which produced morphologically identical spores to those described above, and ITS/ACT loci sequencing yielded sequences identical to those of CT58-1. Spore production and plant inoculations were repeated with this new isolate, and identical symptoms were observed. This is the first report of Neopestalotiopsis infecting strawberries in New England and given the high disease incidence in the initial infected field and relative lack of disease in a neighboring field, it is likely that this pathogen was introduced on bare root plants. As the plants were sourced from a nursery in Ontario, Canada, it is likely that the pathogen is capable of overwintering in the Northeastern United States.

Nutritional Value of Coloured Flesh Potato Tubers in Terms of Their Micronutrient Content

The aim of this study was to compare Fe, Zn, Mn, Al and Li contents in table potato tubers, obtained from seven potato cultivars with different flesh colour, and one cultivar characterised by light-coloured flesh. The study material consisted of potato tubers produced in a one-factor field experiment carried out at the Agricultural Experimental Station in Zawady belonging to the University of Siedlce, Poland (52°03′ N, 22°33′ E) in 2021–2023. The following cultivars were tested: Eurostar (light yellow flesh), Rote Emmalie and Herbie 26 (red flesh), Provita, Salad Blue, Blaue Annelise, Vitelotte Noire and Bora Valley (purple and blue flesh). The study showed that the genetic characteristics of the cultivars determined the nutrient content and uptake with tuber yield. Of the tested minerals which are important for human nutrition (Fe, Zn, Mn), red and purple cultivars accumulated more Fe than the light yellow-fleshed Eurostar. Zn content was the highest in red-fleshed cultivars compared with light and purple-fleshed cultivars. Mn content was the highest in the tubers of the light yellow-fleshed Eurostar and lowest in red Rote Emmalie. Fe, Zn, Mn and Al contents and uptake with tuber yield were influenced by weather conditions during the growing season.

Establishment of breeding research of the genus Citrus in the subtropics of the Russian Black Sea coast

The article briefly describes the history behind the creation of the Sochi Agricultural and Horticultural Experimental Station (now known as the Federal Research Center ‘the Subtropical Scientific Centre of the Russian Academy of Sciences’), as well as the techniques and methods of breeding research to create new varieties of the genus Citrus. A brief biography of the breeder Fyodor Zorin (1904-1967) is presented, as well as the varieties of citrus crops created by him. The article tells the story behind the Friendship Tree, its importance in promoting the ideas of peace and friendship of peoples, and how it became a unique botanical garden.

CORN FOR GRAIN IN CONTINUOUS GROWING UNDER DIFFERENT FERTILIZER SYSTEMS AND WEATHER CONDITIONS

On the experimental field of the Poltava State Agricultural Experimental Station named after M. I. Vavilov of the The Institute of Pig Breeding and Agro-Industrial Production of the National Academy of Agrarian Sciences of Ukraine, during 1984-2023, on typical chernozem in conditions of unstable moisture of the Left-Bank Forest-Steppe, were conducted researches to study the continuous growing of corn for grain and its productivity. Precipitation is the leading factor in providing corn with available moisture in this region. Different hydrothermal conditions, especially the amount of moisture during the agricultural year and the vegetation, led to sharp fluctuations in grain yield over the years, which occurred in a wide range. The analysis of the average values ​​of corn productivity for each 10 consecutive years of conducting these studies made it possible to establish that during the first decade of observations, the dynamics of this indicator in subsequent periods took place in the paradigm of its growth, albeit at a different level. Maize grain yield and its correlation with different weather conditions and fertilization systems, regardless of the replacement of maize hybrids, ranged from direct to inverse and from low to high. Long-term application of different doses and ratios of organic and mineral fertilizers contributed to changes in both quantitative and qualitative indicators of humus in the soil. It was determined that the content of total carbon in the fertilized areas was higher by 3,0 and 3,3 relative percent compared to the unfertilized ones. Special attention was paid to the ratio between humic and fulvic acids, which directly depends on the fertilization system. The different effect of this agrotechnical measure on the amount of humus accumulation in the soil is shown. Thus, in areas without fertilizers (control), its accumulation occurs at the level of an average indicator and a weak effect of organic acids on the mineral part of the soil, while under the organo-mineral fertilization system, this process takes place intensively, and the mineral part remains almost unchanged.

Nitrogen reduction by 20 % with green manure retention reduces soil evaporation, promotes maize transpiration and improves water productivity in arid areas

ContextThe contradiction between high agricultural yields and water resource shortages is becoming increasingly serious. Reducing nitrogen appropriately with green manure retention can ensure high agricultural yields, but it remains unclear whether it can optimize crop water consumption characteristics and improve water productivity (WPc). ObjectiveThis study aims to investigate the effect of green manure retention combined with nitrogen reduction on water use characteristics of maize and its potential mechanisms. MethodsA field experiment was conducted at Wuwei Oasis Agricultural Experimental Station in China from 2020 to 2022, with five treatments: (i) traditional nitrogen application with green manure retention (N100), (ii) nitrogen reduction by 10 % with green manure retention (N90), (iii) nitrogen reduction by 20 % with green manure retention (N80), (iv) nitrogen reduction by 30 % with green manure retention (N70), and (v) nitrogen reduction by 40 % with green manure retention (N60). ResultsThe results showed that compared with the N100 treatment, 0−50 cm maize root biomass (RB), biomass accumulation (BA), and canopy cover (CC) were not significantly reduced in the N80 treatment. This model ensured a stable grain yield (GY) and improved 0−50 cm soil water storage (SWS) by 7.1−13.1 %. It effectively suppressed soil evaporation (E), promoted maize transpiration (T), reduced maize evapotranspiration (ET) by 4.8 %, and increased WPc by 1.8−26.6 %. Structural equation modeling (SEM) suggests that RB was the most important factor for improving WPc. In addition, this system ensures that economic benefits did not decrease. ConclusionIt can be seen that nitrogen reduction by 20 % with green manure retention is a reasonable nitrogen fertilizer management system to alleviate the contradiction between high maize yield and water resource shortages in the region. ImplicationsThe results of this study provide valuable insights into alleviating the contradiction between agricultural production and regional water resource scarcity.

Relationship between the root processing method and quality of herbal medicine of Rauvolfia serpentina cultivated in Japan

Rauvolfia serpentina roots are used as herbal medicine for snake and insect bites, fever, malaria, abdominal pain, hypertension, and dysentery in Southeast Asian countries. This study evaluates the content of reserpine, ajmaline, and ajmalicine in R. serpentina roots obtained with an efficient root processing method. The plants were cultivated in Tanegashima Island in southern part of Japan, where NIBIOHN's experimental farm station is located. We examined both the type of cork layer removal method (manual work, drum mixer washing, and high-pressure washing) and dried roots. We found that the resulting active ingredient contents were not significantly different between manual work and 30 min of drum mixer washing, and high-pressure washing. The washing in a drum mixer for 30 min could be an efficient root processing method. The amount of each active ingredient was compared in each root part (large-, middle-, and small-diameter roots), and there were no significant difference in their contents. In conclusion, R. serpentina roots having a diameter ≥ 0.5 cm contain a similar amount of active ingredients, suggesting that the quality of the crude drug does not vary in the root part. Thus, our study provides significant insights into the use of R. serpentina roots for herbal medicine applications as well as constituents of active ingredients.

EVALUATE THE EFFECT OF SALVIA OFFICINALIS AND CARDAMOM ON ANIMAL PRODUCTIVITY OF BROILER CHICKENS

It is in considerable demand to produce poultry meat and eggs of superior quality at affordable costs without using growth promoters or antibiotics, so the current study aimed to evaluate the effect of Salvia officinalis and Cardamom on animal productivity of broiler chickens. This experiment was carried out at the poultry field of the Agricultural Experiment Station, College of Agriculture, Tikrit University. Ninety unsexed, one-day-old Ross 308 broiler chicks were employed. The results showed that the hemoglobin concentration, RBC counts and PCV of S. officinalis boilers and cardamom boilers indicated a significant (P≤0.05) elevated compared to the standard diet boilers. While, WBC count of S. officinalis boilers and cardamom boilers indicated non-significant (P≤0.05) elevated compared to the standard diet boilers. For antioxidant enzymes, the glutathione and catalase levels of S. officinalis boilers and cardamom boilers indicated a significant (P≤0.05) elevated compared to the standard diet boilers. Finally, total protein and albumin activities of S. officinalis boilers and cardamom boilers indicated a significant (P≤0.05) reduced compared to the standard diet boilers. It is concluded from the current study that both S. offcinalis and cardamom showed a stimulating effect on blood parameters and raising protein levels. S. offcinalis and cardamom can be considered an enhancer of the performance of broiler chickens.

Assessment of the genetic diversity of the alleles of gliadin-coding loci in common wheat (Triticum aestivum L.) collections in Kazakhstan and Russia.

The study of genetic resources using prolamin polymorphism in wheat cultivars from countries with different climatic conditions makes it possible to identify and trace the preference for the selection of the alleles of gliadine-coding loci characteristic of specific conditions. The aim of the study was to determine the "gliadin profile" of the collection of common wheat (Triticum aestivum L.) from breeding centers in Russia and Kazakhstan by studying the genetic diversity of allelic variants of gliadin-coding loci. Intrapopulation (μ ± Sμ) and genetic (H) diversity, the proportion of rare alleles (h ± Sh), identity criterion (I) and genetic similarity (r) of common wheat from eight breeding centers in Russia and Kazakhstan have been calculated. It has been ascertained that the samples of common wheat bred in Kostanay region (Karabalyk Agricultural Experimental Station, Kazakhstan) and Chelyabinsk region (Chelyabinsk Research Institute of Agriculture, Russia) had the highest intrapopulation diversity of gliadin alleles. The proportion of rare alleles (h) at Gli-B1 and Gli-D1 loci was the highest in the wheat cultivars bred by the Federal Center of Agriculture Research of the South-East Region (Saratov region, Russia), which is explained by a high frequency of occurrence of Gli-B1e (86 %) and Gli-D1a (89.9 %) alleles. Based on identity criterion (I), the studied samples of common wheat from different regions of Kazakhstan and Russia have differences in gliadin-coding loci. The highest value of I = 619.0 was found when comparing wheat samples originated from Kostanay and Saratov regions, and the lowest I = 114.4, for wheat cultivars from Tyumen and Chelyabinsk regions. Some region-specific gliadin alleles in wheat samples have been identified. A combination of Gli-A1f, Gli-B1e and Gli-Da alleles has been identified in the majority of wheat samples from Kazakhstan and Russia. Alleles (Gli-A1f, Gli-A1i, Gli-A1m, Gli-A1o, Gli-B1e, Gli-D1a, Gli-D1f, Gli-A2q, Gli-B2o, and Gli-D2a) turned out to be characteristic and were found with varying frequency in wheat cultivars in eight regions of Russia and Kazakhstan. The highest intravarietal polymorphism (51.1 %) was observed in wheat cultivars bred in Omsk region (Russia) and the lowest (16.6 %), in Pavlodar region (Kazakhstan). On the basis of the allele frequencies, a "gliadin profile" of wheat from various regions and breeding institutions of Russia and Kazakhstan was compiled, which can be used for the selection of parent pairs in the breeding process, the control of cultivars during reproduction, as well as for assessing varietal purity.

Jerusalem Artichoke: Energy Balance in Annual and Perennial Cropping Systems—A Case Study in North-Eastern Poland

This article presents the results of a three-year experiment (2018–2020) conducted at the Agricultural Experiment Station in Bałcyny (north-eastern Poland) with the aim of determining Jerusalem artichoke (JA) yields and the energy balance of biomass production in (i) a perennial cropping system (only aerial biomass was harvested each year) and (ii) an annual cropping system (both aerial biomass and tubers were harvested each year). When JA was grown as a perennial crop, the demand for energy reached 25.2 GJ ha−1 in the year of plantation establishment and 12.3–13.4 GJ ha−1 in the second and third year of production. The energy inputs associated with the annual cropping system were determined in the range of 31.4–37.1 GJ ha−1. Biomass yields were twice as high in the annual than in the perennial cropping system (20.98 vs. 10.30 Mg DM ha−1). Tuber yield accounted for 46% of the total yield. The energy output of JA biomass was 1.8 times higher in the annual than in the perennial cropping system (275.4 vs. 157.3 GJ ha−1). The average energy gain in JA cultivation ranged from 140 (perennial crop) to 241 GJ ha−1 (annual crop). The energy efficiency ratio of JA biomass production reached 7.7–13.3 in the perennial cropping system, and it was 20% lower in the annual cropping system. These results imply that when JA was grown as an annual crop, an increase in energy inputs associated with plantation establishment (tillage and planting) and the harvest and transport of tubers was not fully compensated by the energy output of tubers.

The study of potato hybrids in the Kola North

The article presents data on the study of potato varieties (2020–2022) in the Murmansk region on the basis of an integrated scientific research institution Murmansk State Agricultural Experimental Station – a branch of the VIR. In the trial dig, the hybrid 211/1 was isolated, which exceeded in all main indicators (yield, marketability, mass of tubers from one bush, mass of one tuber) the standard sample – the most adapted to the conditions of the Kola North (variety Elizabeth). There were good results in the main cleaning in terms of marketability: in 2020: hybrid 211/1 – 98.9%, hybrid 114/11 – 98.6, hybrid 2103/7 – 99.4, hybrid 211/9 – 99.4%; in 2021. – 211/1 – 98,1, 114/11 – 96,3, 2103/7 – 98,4, 211/9 – 99,1%; in 2022. -211/1 – 99,5, 114/11 – 99,8, 2103/7 – 99,2, 211/9 – 99%. In 2020 and 2021, the hybrid 211/1 stood out among the other four in terms of yield, marketability, weight of tubers from one bush, weight of one tuber. In 2022, hybrids 114/11 and 211/9 had the best yields. The mass of one tuber in the main harvest in 2022 for the hybrid 114/11 is 126.3 g (more than the standard). According to the maturation time, all samples are close to the standard. According to the research results, the most adapted hybrid 114/11 can be recommended to the population of the northern region.

Registration of ‘UC‐Alameda’, a California adapted, non‐glycosidic nitrile‐producer, two‐row spring malting barley

AbstractThe emerging malting industry in California needs adapted cultivars for a sustainable local barley production. ‘UC‐Alameda’ (Reg. no. CV‐381, PVP 202300263, PI 701932) is a two‐row, spring malting barley (Hordeum vulgare L.) released by the University of California, Davis, Agricultural Experiment Station, in 2022. UC‐Alameda is a highly productive cultivar with satisfactory malting quality for local craft maltsters. UC‐Alameda is adapted to the California Central Valley (Sacramento and San Joaquin Valleys) and is resistant to the most common diseases present in this region (e.g., Barley yellow dwarf virus, Cereal yellow dwarf virus, stripe rust, and powdery mildew) and moderately resistant to scald and net blotch. It was evaluated in preliminary trials, as B9K62, from 2017 to 2023 at Davis, CA, and by the University of California Regional Small Grains Testing program, as UC1911, from 2018 to 2023 for late fall planting in the Central Valley. UC‐Alameda is a non‐glycosidic nitrile producer, which is an important trait for craft‐maltsters and distillers. UC‐Alameda satisfies the quality criteria of the local craft malting and brewing industry interested in sourcing locally grown malting barley.

Registration of FloRun ‘T61’ peanut

AbstractFloRun ‘T61’ (Reg. no. CV‐159, PI 700039) is a high‐oleic, runner market‐type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) cultivar developed by the University of Florida Agricultural Experiment Station, North Florida Research and Education Center, near Marianna, FL. It resulted from a cross between ‘Georiga‐06G’ and a University of Florida Agricultural Experiment Station experimental line ‘UF12302’ and was tested under the experimental designation UF11 × 41‐HO1‐4‐1‐1 and was approved for release in 2021. FloRun ‘T61’ has a semi‐prostrate growth habit, with moderate vine size and a prominent center stem. The seed is smaller than seed of Georgia‐06G but larger than FloRun ‘331’ (67.3 g vs. 64.6 g; P < 0.0001). It yielded similarly to FloRun ‘331’ over the 4‐year period of 2017–2020 in three Florida locations (7862 kg ha−1 vs. 8068 kg ha−1), and its pod yield was slightly greater than Georgia‐06G during the 5‐year period of 2016–2020 (7680 kg ha−1 vs. 7273 kg ha−1; P = 0.0137). The total sound mature kernel grade of FloRun ‘T61’ was slightly greater than that of FloRun ‘331’ (78.6% vs. 77.7%, P < 0.025) but was similar to the total sound mature kernel grade of Georgia‐06G. FloRun ‘T61’ has high oleic oil chemistry composed of 77.1% oleic fatty acid versus 58.7% for Georgia‐06G in 2020 (P < 0.001) and 79.2% in 2021 compared with 65.7% for Georgia‐06G (P < 0.001). Over 5 years, the spotted wilt ratings of FloRun ‘T61’ (2.5) were less than for FloRun ‘331’ (4.8; P < 0.01). In summary, FloRun ‘T61’ combines excellent yield, grade, disease, and oil chemistry traits and should be beneficial to farmers and manufacturers of peanut foods.

Registration of the “Woodies” multi–rust‐resistant barley germplasm

AbstractSelection for resistance to plant diseases is a continuous effort on the part of plant breeders. Sources of genetic resistance are often limited, despite considerable discovery efforts. Stem rust and stripe rust are two diseases of particular importance in barley (Hordeum vulgare L.) production. The present work aims to develop and deploy genotypes with resistance to these diseases that can be used in future breeding efforts. The Woodies, Woody‐1 (DH160733; Reg. no. GP‐218, PI 704479) and Woody‐2 (DH160754; Reg. no. GP‐219, PI 704480), are two doubled‐haploid genotypes produced via F1 anther culture named in honor of the late Lynn “Woody” Gallagher. These two‐row spring habit barley germplasm accessions were released by the Oregon Agricultural Experiment Station in 2023. These genotypes have demonstrated resistance to both stem and stripe rust at the seedling and adult plant stage in trials conducted between 2018 and 2023. The genetic basis of this resistance appears to be a novel quantitative trait locus conferring resistance to both diseases on chromosome 5H that is different from the known rpg4/Rpg5 complex for stem rust resistance found on the same chromosome. Seed can be requested from the Oregon State University Barley Breeding Program or from the NLGRP Germplasm repository.

Establishment of Anoxia/cytokine-induced Injury and Regeneration in Colonoids

Intestinal epithelial cells (IECs) play key roles in nutrient absorption and fluid secretion, providing a barrier to protect cells. IECs, derived from intestinal stem cells (ISCs), are crucial in maintaining intestinal homeostasis and regeneration, which is crucial in gastrointestinal diseases such as inflammatory bowel disease (IBD). To study epithelial injury/regeneration, primary cultured colonoids were utilized as a model system to mimic injury. They retain the composition and differentiation of the tissue, making it a suitable model for studying homeostasis and regeneration of ISCs in vitro. Anoxia and proinflammatory cytokines frequently coexist and exacerbate one another during intestinal injury. However, anoxic conditions have been mainly ignored in previous research. Thus, we developed a regeneration model using cytokines/anoxia injury to better recapitulate intestinal inflammation. Colonoids were treated with cytokines TNF-α and IFN-γ (5 ng/μL) in anoxic conditions for 24 hours to provoke an injury. After 24 hours of injury, the colonoids were exposed to normal oxygen and media conditions for regeneration. Regeneration was observed for 24, 48, and 72 hours and documented using photomicrographs. Morphological analysis detailed that injury was most severe 24 hours post-injury with the combined induction of cytokines and anoxia resulting in decreased colonoid area and number of buds compared to control groups. RT-PCR analysis detailed mRNA expression of stem cell markers ( Lgr5, Bmi1, and Hopx), Wnt ( Axin2, Myc2, and Ascl2), and YAP target genes ( Sca1 and Ctgf) were increased at peak injury and returned to pre-injury levels at 72 hours of recovery. Regeneration of the colonoids is consistent with in vivo observations, thus confirming the validity of the model. This versatile model can be used to study responses to injury and regeneration of intestinal epithelium and the function of ISCs with applications in intestinal disease therapeutics. University of Maryland College of Agriculture and Natural Resources and the Maryland Agricultural Experiment Station Undergraduate Research Grant Grant #4307572 from the Maryland Stem Cell Research Fund. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.