Milk Agent Research Articles

A Comprehensive Review of General Characteristics of Peptides of Serum Immunoglobulins and Their Health Benefits

As we know Immunoglobulin or antibodies are kind of protein which is produced by the immune system and fight against microbes or germs. Immunoglobulins are: IgM, IgG and IgA which supply long-term and short-term defense against contamination or infection. Several studies had found that the milk immunoglobulins are able to improve immune defense system response against germs and make available passive immunity, specially, in infants and young animals. The existence of immunologic agents in milk, like lactoferrin and lysozyme, can assist more to its protective effects. And also by incorporation of immunoglobulins and other immune improving materials to the formula milk, we can supply or make ready for the infants the same immunologic benefits like those which got from breastfeeding. We can say that this approach is beneficial for all infants specially it is beneficial for babies that are not able to feed from breast or have compromised Immune Systems.

Side-stream lignins: Potential antioxidant and antimicrobial agents in milk

In recent years, lignin has drawn increasing attention due to its intrinsic antibacterial and antioxidant activities, biodegradability, and biocompatibility. Yet, like several other biogenic structures, its compositional heterogeneity represents a challenge to overcome. In addition, there are few studies regarding food applications of lignin. Herein, we evaluate the antimicrobial and antioxidant effects of lignin from two different sources. These lignins were characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR) and hydrogen nuclear magnetic resonance (1H NMR) spectroscopies. Their antibacterial and antioxidant capacities (DPPH and Folin-Ciocalteu methods) were also investigated. Susceptibility tests were performed with the minimal inhibitory (MIC) and bactericidal (MBC) concentrations using the micro-broth dilution technique. Kraft lignin presented higher radical-scavenging and antibacterial activities than alkali lignin, indicating the dependence of antioxidant and antibacterial activities on the precursor biomass. Scanning electron microscopy shows morphologic changes in the bacteria after exposure to lignin, while confocal microscopy suggests that kraft lignin has affinity towards bacterial surfaces and the ability to cause cell membrane destabilization. Lignin inhibited the growth of Staphylococcus aureus and Salmonella Enteritidis in skimmed milk, herein taken as food model. Our results suggest that lignins are promising candidates for green additives to improve quality and safety within the food chain.

Onopordum nervosum ssp. platylepis Flowers as a Promising Source of Antioxidant and Clotting Milk Agents: Behavior of Spontaneous and Cultivated Plants under Different Drying Methodologies

This study is a contribution to the valorization of the Onopordum nervosum ssp. platylepis flowers in the agri-food sector. It aims to evaluate the effect of different drying methodologies (room, microwave, convective, oven and freeze-drying) on the chemical composition, the microbiological quality of aqueous extracts and their biological activities, namely clotting milk and antioxidant activities for both spontaneous and cultivated plants. The results showed that the drying methodology has significantly affected the phenolic composition. The extract obtained from freeze-dried flowers showed the best amounts of total phenols (6.19 ± 0.50 mg GAE/g), flavonoids (2.35 ± 0.07 mg QE/g) and condensed tannins (0.038 ± 0.002 mg CE/g) followed by the microwave-dried flowers. However, microwave-dried flowers presented the lowest protein content with only 3.58 ± 0.62 mg BSAE/g. Finally, the biological activities were significantly dependent on the used drying process. The extract of freeze-dried flowers was the most potent to inhibit free radicals (diammonium 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH)) and to reduce iron with the efficient concentrations EC50s of 0.48 ± 0.02 mg/mL, 0.16 ± 0.04 mg/mL and 1.52 ± 0.19 mg/mL, respectively. It also showed the highest clotting milk activity followed by room-dried and forced convective-dried flowers. As a consequence, freeze drying was the best method for preserving most chemical and biological properties of Onopordum nervosum ssp. platylepis flowers. However, in consideration of production costs and drying duration, the convective dryer is recommended as a more practical choice. All the obtained results do not reveal a significant difference between cultivated and spontaneous plants, which can suggest that the domestication of Onopordum nervosum ssp. platylepis can be useful for industrial applications.

Mouse mammary tumor virus is implicated in severity of colitis and dysbiosis in the IL-10−/− mouse model of inflammatory bowel disease

BackgroundFollowing viral infection, genetically manipulated mice lacking immunoregulatory function may develop colitis and dysbiosis in a strain-specific fashion that serves as a model for inflammatory bowel disease (IBD). We found that one such model of spontaneous colitis, the interleukin (IL)-10 knockout (IL-10−/−) model derived from the SvEv mouse, had evidence of increased Mouse mammary tumor virus (MMTV) viral RNA expression compared to the SvEv wild type. MMTV is endemic in several mouse strains as an endogenously encoded Betaretrovirus that is passaged as an exogenous agent in breast milk. As MMTV requires a viral superantigen to replicate in the gut-associated lymphoid tissue prior to the development of systemic infection, we evaluated whether MMTV may contribute to the development of colitis in the IL-10−/− model.ResultsViral preparations extracted from IL-10−/− weanling stomachs revealed augmented MMTV load compared to the SvEv wild type. Illumina sequencing of the viral genome revealed that the two largest contigs shared 96.4–97.3% identity with the mtv-1 endogenous loci and the MMTV(HeJ) exogenous virus from the C3H mouse. The MMTV sag gene cloned from IL-10−/− spleen encoded the MTV-9 superantigen that preferentially activates T-cell receptor Vβ-12 subsets, which were expanded in the IL-10−/− versus the SvEv colon. Evidence of MMTV cellular immune responses to MMTV Gag peptides was observed in the IL-10−/− splenocytes with amplified interferon-γ production versus the SvEv wild type. To address the hypothesis that MMTV may contribute to colitis, we used HIV reverse transcriptase inhibitors, tenofovir and emtricitabine, and the HIV protease inhibitor, lopinavir boosted with ritonavir, for 12-week treatment versus placebo. The combination antiretroviral therapy with known activity against MMTV was associated with reduced colonic MMTV RNA and improved histological score in IL-10−/− mice, as well as diminished secretion of pro-inflammatory cytokines and modulation of the microbiome associated with colitis.ConclusionsThis study suggests that immunogenetically manipulated mice with deletion of IL-10 may have reduced capacity to contain MMTV infection in a mouse-strain-specific manner, and the antiviral inflammatory responses may contribute to the complexity of IBD with the development of colitis and dysbiosis.8hbTGJzMzAHuPYE9uy6yoBVideo

Microbial antigen in human milk: a natural vaccine?

Microbial antigen in human milk: a natural vaccine?

Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk

Food safety is very important in the food industry as most pathogenic bacteria can cause food-borne diseases and negatively affect public health. In the milk industry, contamination with Salmonella has always been a challenge, but the risks have dramatically increased as almost all bacteria now show resistance to a wide range of commercial antibiotics. This study aimed to isolate a bacteriophage to be used as a bactericidal agent against Salmonella in milk and dairy products. Here, phage ZCSE6 has been isolated from raw milk sample sand molecularly and chemically characterized. At different multiplicities of infection (MOIs) of 0.1, 0.01, and 0.001, the phage–Salmonella interaction was studied for 6 h at 37 °C and 24 h at 8 °C. In addition, ZCSE6 was tested against Salmonella contamination in milk to examine its lytic activity for 3 h at 37 °C. The results showed that ZCSE6 has a small genome size (<48.5 kbp) and belongs to the Siphovirus family. Phage ZCSE6 revealed a high thermal and pH stability at various conditions that mimic milk manufacturing and supply chain conditions. It also demonstrated a significant reduction in Salmonella concentration in media at various MOIs, with higher bacterial eradication at higher MOI. Moreover, it significantly reduced Salmonella growth (MOI 1) in milk, manifesting a 1000-fold decrease in bacteria concentration following 3 h incubation at 37 °C. The results highlighted the strong ability of ZCSE6 to kill Salmonella and control its growth in milk. Thus, ZCSE6 is recommended as a biocontrol agent in milk to limit bacterial growth and increase the milk shelf-life.

The traditional dairy value chain in Zambia and potential risk factors to microbiological food safety

Milk is a highly valuable food for a balanced nutrition and contributes to food security in Sub-Saharan African countries like Zambia. The traditional dairy value chain accounts for a large share of Zambia's total milk supply. Comprehensive surveys yielding in around 400 filled-in questionnaires were carried out among traditional/smallholder farmers, milk collection centres (MCCs), informal traders, but also supermarkets/shops, and consumers in three Zambian provinces (Lusaka, Southern, Western). Using individual surveys for each stakeholder group, data were collected on general information and management practices, cattle disease management, hygienic measures, milk-handling practices, milk quality checks and backtracing, food safety knowledge, and milk consumption. The study provides insights into the traditional dairy chain and differences between the study provinces. For example, smallholder farms with high-yielding cattle breeds were dominating in the Lusaka Province, and here the MCCs received relatively large amounts of milk. In contrast, the surveyed farms in the Southern and Western Provinces were dominated by traditional cattle breeds, and the MCCs in the Western Province were the smallest. Distances for milk transport by farmers were largest in the Western Province, and here transport by foot was still common. Unrefrigerated storage and transport that favour proliferation of zoonotic agents in raw milk were frequently observed. Moreover, the survey uncovered hygiene deficiencies, particularly on farm level, for traders, and for consumers, which may further cause microbial contamination. Consumption of raw milk and products thereof was frequently identified and poses a health risk. Knowledge about food hygiene and safety needs to be improved along the Zambian dairy value chain.

Environmentally friendly production method of virgin coconut oil using enzymatic reaction

Virgin Coconut Oil (VCO) has been known in Indonesia since a decade ago. This oil is made from coconut milk using unheated method, such as enzymatic, physical, and chemical method. The VCO has been proofed to have many health benefits such as for treatment of hyper-cholesterol, diabetes, viral and bacterial infections. Also, the oil has been utilized in the cosmetics industry for beauty skin care. In the increase of VCO demand for pharmaceutical industry, this study was conducted to find the method for VCO production using an environmentaly friendly process. The environmentally friendly process was conducted to maintain the sustainable environment. This paper deals with the enzymatic method of VCO production. The enzyme was obtained from fresh pineapple juice that is proofed contain bromelin. The enzyme will cleave the polypeptide bond of emulsifying agent in the coconut milk. Further, the oil will be separated and then collected as the VCO. During this work, the amount of the fresh pineapple juice varied to measure the rate of VOC production. Based on the observation during the production, it is found that the increase of fresh pineapple juice added increases the VCO production.

HEAVY METALS IN MILK INFANT FORMULAS

Baby food can contain harmful components that are very dangerous to little consumers. One of the most common harmful factors is heavy metals, in particular lead. It is known that introducing one extra microelement into the body changes not only the concentration of this element, but also the concentration of other elements. Disturbing the physiological balance of microelements leads to changes in complex enzymatic processes, which in turn violates the structure of tissue cells of individual organs. Studies by Russian scientists (T. Suldina and others) have determined, in milk for newborns, the maximum levels of lead (0.02 mg/kg), of cadmium (0.02 mg/kg), of arsenic (0.05 mg/kg). While studying certain samples of baby food, the method for determining the content of lead and other heavy metals was improved. Depending on the lead concentration, characteristic lines of both the ultraviolet and the visible spectral regions can be used. Monitoring heavy metals in infant milk has shown that lead, copper, and zinc are the main contaminants of the product. The concentration of toxic agents in baby milk depends on the type of raw materials and on the environmental conditions of their manufacture. To detect lead in products quickly and accurately, a method was used based on the emission spectral analysis. To this end, spectrographs ISP-28 and ISP-30 widely employed in industry and laboratory studies were taken, but the detection limits were lowered compared to those in the methods that had been used previously. Besides, the field-proven method for determining lead is applicable to detecting zinc and copper, too. An excess of lead by 0.08 mg/kg, copper by 0.07 mg/kg, zinc by 0.45 mg/kg was observed in the baby milk “Malyatko Premium 2.” In the milk “Bellakt 2,” the excessive amount of copper was 0.05 mg/kg, of zinc 0.20 mg/kg, of lead 0.05 mg/kg. In the baby milk “Nitricia Malyuk Istrynsky,” only zinc was found to exceed the normal level (by 0.1 mg/kg). The baby milks “Nutricia Milupa 2,” “Nestle Nestogen 2,” “HiPP Organic 2” do not contain excessive heavy metals, and thus, can be recommended as safe for consumption.

Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus

The aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with ˂ 1000 CFU/mL of S. aureus or SCM2 group (n = 55) with ≥1000 CFU/mL of causative agent in milk. Significantly lower serum concentrations of high density lipoprotein-cholesterol (HDLC) in SCM2 group and higher low density lipoprotein-cholesterol (LDL-C) in both SCM groups were recorded. Significantly lower paraoxonase-1 (PON1) activity and higher lipid hydroperoxides (LOOH) concentration in blood and milk were recorded in both groups of SCM cows vs control. In blood serum of cows from SCM2 group significantly higher concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), but significantly lower total antioxidative capacity (TAC) were obtained. In milk serum of SCM cows were detected significantly higher concentrations of H2O2 and MDA, but significantly lower TAC vs control. There was a significant positive correlation between PON1 in serum with HDLC, but negative correlation with LDL-C and LOOH. In milk, PON1 negatively correlated with LOOH and somatic cell counts (SCC). Strong positive correlation was obtained between PON1 in blood and milk. Oxidative stress and inflammatory reaction induced by SCM significantly lowered PON1 activity in blood and milk of affected cows.

Use of thyme essential oil as an antibacterial agent in raw milk intended for the production of farm cheese

This study aims to use thyme essential oil (TEO) as an antibacterial agent in raw milk intended for the production of farm cheese and to test its effect on bacteriological and sensory quality. Antibacterial activity of TEO was first tested in vitro on four strains of bacteria known for their pathogenicity such as Escherichia coli , Pseudomonas aeruginosa , Salmonella typhimurium and Staphylococcus aureus and then in vivo on the total mesophilic aerobic flora (TMAF) in raw milk used to produce farm cheese. All tested bacteria were very sensitive to TEO with a higher sensitivity for Escherichia coli , followed by Pseudomonas aeruginosa , then Staphylococcus aureus and finally Salmonella tiphymirium. In vivo study showed that TEO has an effective effect on TMAF of raw milk. Monitoring bacteriological quality of TEO based farm cheese during 6 days of cold storage, revealed the effectiveness of TEO on TMAF, yeasts and moulds. Despite the good bacteriological quality of TEO-based farm cheese, the strong aroma characterizing this essential oil was not well appreciated by some tasters, which had a negative impact on the final score attributed to this cheese. Thyme essential oil can be used as an alternative solution to produce healthy farm cheese without needing heat treatment, while ensuring high nutritional value food product.

The pathology of tuberculosis lesions in allergic skin test (PPD tuberculin) positive cows and detection of tuberculosis agents with PCR in milk samples

The aim of this study was to determine tuberculosis (Tb) agents in milk and the pathologic changes in the tissues and organs of 100 PPD Tb-positive cows that were intended for slaughter. Milk samples were taken before slaughtering and PCR analysis was conducted to detect the Tb agent. In postmortem examinations, the lungs, liver, mammary glands, spleen, kidneys, and heart were collected for macroscopic and histopathological examinations. Out of the 100 PPD positive cows, lesions of Tb were observed in 75 cows. In the microscopic examination, Tb was identified in the lungs (51 cases), liver (61 cases), and in both the liver and lungs (37 cases). Although mammary Tb was suspected in 32 cows in macroscopic examinations, in microscopic examinations these cases were evaluated as chronic pyogenic abscesses mastitis. No Tb agents were detected by PCR analysis in milk samples taken from the cows before slaughter. Therefore, periodical milk inspections are suggested for controlling agents in milk. In some of the Tb PPD positive animals (25 cases), no changes were detected; therefore, there is a need to improve tests with higher sensitivity for the antemortem diagnosis of Tb. Moreover, people involved in the livestock sector should be trained about Tb, hygiene rules, and disease-free herds.

Exposure Concentrations of Infants Breastfed by Women Receiving Biologic Therapies for Inflammatory Bowel Diseases and Effects of Breastfeeding on Infections and Development

Exposure to biologic and immunosuppressant agents during breastfeeding is controversial, and there are limited data on safety. We investigated whether biologics are detectable in breast milk from women receiving treatment for inflammatory bowel diseases (IBDs) and whether breastfeeding while receiving treatment is associated with infections or developmental delays. We performed a multicenter prospective study of women with IBD and their infants, collecting breast milk samples (n= 72) from patients receiving biologic therapy from October 2013 to November 2015. Drug concentrations were measured in all breast milk samples at several time points within 48 hours of collection and within 168 hours for some samples. Child development was assessed using the Ages and Stages Questionnaire 3, completed by 824 women with IBD (treated or untreated) during pregnancy (620 breastfed, and 204 did not). Data on children's health and development were obtained from mothers and pediatricians, along with information on mothers' medication exposure, IBD history, activity, pregnancy, and postpartum complications. We used chi-squared method or Fisher exact test to determine associations between categorical values and compared differences in continuous outcomes between groups using analysis of variance models. The primary outcome was drug concentration of biologic agents in breast milk (from 72 women) at 1, 12, 24, and 48 hours after dosing and also at 72, 96, 120, and 168 hours for available samples. Secondary outcomes were a range of infant infections and Ages and Stages Questionnaire 3-defined developmental delays among all breastfed infants. We detected infliximab in breast milk samples from 19 of 29 treated women (maximum, 0.74μg/mL), adalimumab in 2 of 21 treated women (maximum, 0.71 μg/mL), certolizumab in 3 of 13 treated women (maximum, 0.29 μg/mL), natalizumab in 1 of 2 treated women (maximum,0.46 μg/mL), and ustekinumab in 4 of 6 treated women (maximum, 1.57 μg/mL); we did not detect golimumab in breast milk from the 1 woman receiving this drug. Rates of infection and developmental milestones at 12 months were similar in breastfed vs non-breastfed infants: any infection, 39%vs 39% in control individuals (P > .99) and milestone score, 87 vs 86 in control individuals (P= .9992). Rates of infection and developmental milestones did not differ among infants whose mothers received treatment with biologics, immunomodulators, or combination therapy compared with unexposed infants (whose mothers received treatment with mesalamines or steroids or no medication). In a study of women receiving treatment for IBD and their infants, we detected low concentrations of infliximab, adalimumab, certolizumab, natalizumab, and ustekinumab in breast milk samples. We found breastfed infants of mothers on biologics, immunomodulators, or combination therapies to have similar risks of infection and rates of milestone achievement compared with non-breastfed infants or infants unexposed to these drugs. Maternal use of biologic therapy appears compatible with breastfeeding. Clinicaltrials.gov no.: NCT00904878.

The destructive effect of strong concentrations of orthophosphate acid in an acid detergent disinfectant on elements of milking equipment made of stainless steel and aluminum

A huge part of the milking equipment and milk dishes are made of stainless steel and aluminum. Under certain conditions, the influence of such factors as the atmosphere, water, milk, and chemical agents leads to the destruction of metal working surfaces of the milking equipment, resulting in a shorter period of their exploitation. On damaged sites the milk remains are left, that is actually a good nutrient medium, resulting in the creation of conditions for the active development of the microflora. As a result, the following process leads to a marked reduction in disinfectant effect when sanitized. The object of this work was to investigate the influence of various concentrations of orthophosphate acid in an acid detergent disinfectant on the elements of the milking equipment made of stainless steel and aluminum. Accordingly, the result of the conducted research showed that the orthophosphate acid of all four experimental concentrations (20, 25, 30, 35%) in the acid detergent showed a slight destructive effect on stainless steel and was within the standard of corrosion value up to 2 g/m2/year. The increase in the concentration of orthophosphate acid caused a growth in the amount of destruction of stainless steel, which was the highest in the concentration of 35% and amounted to 0.326 g/m2/year. The rate of destruction of stainless steel was in the range from 0.895 ± 0.0036 to 1.712 ± 0.0031 mg/m2/hour and was significantly lower than the norm, which is up to 6.0 mg/m2-year. The magnitude of the destructive action of 20, 25, 30, 35% of the concentration of orthophosphoric acid in an acid mist and disinfectant on the plates of aluminum was higher than the norm, respectively, at 2.42, 2.88, 3.09 and 3.60 times, and the rate of destruction – respectively, at 2.17, 2.63, 2.82 and 3.28 times. Therefore, the addition of a nitric acid ammonium inhibitor in the experimental variants of acidic detergent disinfectant resulted in a decrease in the magnitude of the destruction of aluminum. The results were higher than the norm but were significantly lower (P ≤ 0.05) compared to control, which used «Ecocid», an acid concentration of 30%.

Influence of selenium and vitamin e supplementation during pregnancy on udder health and milk quality in dairy cows at parturition

Selenium and vitamin E ranks among very important antioxidant agents protecting the organism from the effect of reactive oxygen forms. The deficiency of both nutrients during pregnancy in cows often result in metabolic disorders and increased of cases of related diseases (mastitis, retained placenta and other reproductive disorders). The aim of the present work was to study the influence of different dose of parenteral administration selenium and vitamin E in dairy cows prior to parturition on selected metabolic parameters, udder health and milk quality. A total in herd of 270 Holstein cattle in east of Slovakia in a two-four lactation-gestation cycle the control group (C) and 2 experimental groups (D, D1) were selected. All groups were similarly housed, managed and fed with the diet containing from 36 to 42 mg vitamin E and 0.2 mg.kg-1 Se of DM through the study period. In group D a products containing vitamin E and selenium were administered IM four weeks prior to the expected date of parturition in total dose of 1000 mg of dl-α-tocopherol acetate and of 44 mg sodium selenite per cow, respectively. In group D1 the same products were administered twice, four and two weeks prior to parturition. Blood samples were 4 weeks prior to predicted calving date (the time of treatment), on parturition day and at 14th day after calving for assessment of plasma vitamin E and selenium concentrations. Blood samples of the calves were drawn from jugular vein at birth and first colostrum was also collected. The occurrence of the mastitis and retained placenta during the first 14th day after calving were evaluated in all groups. Higher plasmatic and colostral concentrations of selenium and vitamin E were found only in group with repeat application of Se and vitamin E (D1) collected on the day of parturition. At the 14th day of postpartal period a trend of lower occurrence of mastitis was observed in group D1 compared to D group, administered IM once and control group. Parenteral supplementation of selenium and vitamin E during pregnancy had no impact on their transmission into the milk and on the presence of bacterial agents in raw milk obtained from dairy cows diagnosed with mastitis.

Infectious Agents in Bovine Red Meat and Milk and Their Potential Role in Cancer and Other Chronic Diseases.

Red meat and dairy products have frequently been suggested to represent risk factors for certain cancers, chronic neurodegenerative diseases, and autoimmune and cardiovascular disorders. This review summarizes the evidence and investigates the possible involvement of infectious factors in these diseases. The isolation of small circular single-stranded DNA molecules from serum and dairy products of Eurasian Aurochs (Bos taurus)-derived cattle, obviously persisting as episomes in infected cells, provides the basis for further investigations. Gene expression of these agents in human cells has been demonstrated, and frequent infection of humans is implicated by the detection of antibodies in a high percentage of healthy individuals. Epidemiological observations suggest their relationship to the development multiple sclerosis, to heterophile antibodies, and to N-glycolylneuraminic acid (Neu5Gc) containing cell surface receptors.

Measurement and analysis of fluorescent whitening agent content in soybean milk based on image techniques

Abstract This paper presents a method for measuring and quantitatively analyzing the fluorescent whitening agent in soybean milk based on image techniques. After collecting the fluorescent images of the soybean milk samples, the top seven wavelet moment invariants are selected according to the sample training and experimental comparison. Then calculate the standard templates of the 49 classes of calibration samples with different fluorescent whitening agent content ranging from 0.02 mg/ml to 0.5 mg/ml. The minimum distance method is carried out to match the testing sample with the calibration template, which realizes the quantitative analysis. To verify the effectiveness of the presented method, the prediction experiment is carried out. Results show that the absolute errors are within 0.005 mg/ml and the relative errors are within 5%, which means this method can measure the fluorescent whitening agent in soybean milk. This research presents a new approach for detecting the illegal fluorescence additive in food production.

Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage.

Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20°C. Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.

Qualitative screening of veterinary anti-microbial agents in tissues, milk, and eggs of food-producing animals using liquid chromatography coupled with tandem mass spectrometry.

A method for the analysis of 120 drugs in animal derived food was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). These analytes belong to 12 families of veterinary anti-microbial agents (quinolones, macrolides, β-lactams, nitroimidazoles, sulfonamides, lincomycines, chloramphenicols, quinoxalines, tetracyclines, polypeptides, and antibacterial synergists) as well as other compounds not assigned to a particular drug family. The animal derived food samples include muscle and liver of swine, bovine, sheep, and chicken, as well as hen eggs and dairy milk. The sample preparation included ultrasound-assisted extraction (UAE) with acetonitrile-water and a final clean-up with auto solid-phase extraction (SPE) on HLB cartridges. The detection and quantification of 120 anti-microbial agents was performed using LC-MS/MS in positive and negative ion mode. The chromatographic separation was performed on a C18 column using acetonitrile and 0.1% formic acid as the mobile phase. The limit of detection (LOD) and limit of quantification (LOQ) of all drugs in food-producing animals were 0.5-3.0μg/kg and 1.5-10.0μg/kg, respectively. The developed method was successfully utilized to monitor real samples, which demonstrated that it is a simple, fast, and robust method, and could be used as a regulatory to screen for the presence of residues from veterinary anti-microbial drugs in animal-derived foods.

Human Milk Oligosaccharides and Synthetic Galactosyloligosaccharides Contain 3′-, 4-, and 6′-Galactosyllactose and Attenuate Inflammation in Human T84, NCM-460, and H4 Cells and Intestinal Tissue Ex Vivo

Background: The immature intestinal mucosa responds excessively to inflammatory insult, but human milk protects infants from intestinal inflammation. The ability of galactosyllactoses [galactosyloligosaccharides (GOS)], newly found in human milk oligosaccharides (HMOS), to suppress inflammation was not known.Objective: The objective was to test whether GOS can directly attenuate inflammation and to explore the components of immune signaling modulated by GOS.Methods: Galactosyllactose composition was measured in sequential human milk samples from days 1 through 21 of lactation and in random colostrum samples from 38 mothers. Immature [human normal fetal intestinal epithelial cell (H4)] and mature [human metastatic colonic epithelial cell (T84) and human normal colon mucosal epithelial cell (NCM-460)] enterocyte cell lines were treated with the pro-inflammatory molecules tumor necrosis factor-α (TNF-α) or interleukin-1β (IL-1β) or infected with Salmonella or Listeria. The inflammatory response was measured as induction of IL-8, monocyte chemoattractant protein 1 (MCP-1), or macrophage inflammatory protein-3α (MIP-3α) protein by ELISA and mRNA by quantitative reverse transcriptase-polymerase chain reaction. The ability of HMOS or synthetic GOS to attenuate this inflammation was tested in vitro and in immature human intestinal tissue ex vivo.Results: The 3 galactosyllactoses (3′-GL, 4-GL, and 6′-GL) expressed in colostrum rapidly declined over early lactation (P < 0.05). In H4 cells, HMOS attenuated TNF-α– and IL-1β–induced expression of IL-8, MIP-3α, and MCP-1 to 48–51% and pathogen-induced IL-8 and MCP-1 to 26–30% of positive controls (P < 0.001). GOS reduced TNF-α– and IL-1β–induced inflammatory responses to 25–26% and pathogen-induced IL-8 and MCP-1 to 36–39% of positive controls (P < 0.001). GOS and HMOS mitigated nuclear translocation of nuclear transcription factor κB (NF-κB) p65. HMOS quenched the inflammatory response to Salmonella infection by immature human intestinal tissue ex vivo to 26% and by GOS to 50% of infected controls (P < 0.01).Conclusion: Galactosyllactose attenuated NF-κB inflammatory signaling in human intestinal epithelial cells and in human immature intestine. Thus, galactosyllactoses are strong physiologic anti-inflammatory agents in human colostrum and early milk, contributing to innate immune modulation. The potential clinical utility of galactosyllactose warrants investigation.