Intake Of Aflatoxin M1 Research Articles

Human Health Risk Assessment and Exposure Evaluation by Monte-Carlo Simulation Method for Aflatoxin M1 in Widely Consumed Infant Dried Powder Milk in Iran

Monitoring aflatoxin M1 (AFM1) in dairy products and milk-based foods is very important. The main purpose of this research was to investigate and determine the amount and human health risk assessment of aflatoxin M1 (AFM1) in the most famous and widely used brands of infant dried powder milk (IDPM) consumed in Iran. For this study, 45 imported IDPM (IM-IDPM) samples and 45 domestically produced IDPM (DO-IDPM) samples (a total of 90 samples) were selected randomly. All samples were analyzed for AFM1 using a competitive enzyme-linked immunosorbent assay (ELISA) technique. The mean level and the percentage of positive samples for AFM1 in DO-IDPM were 9.2 ± 5.4 ng/kg and 73.3%, and for IM-IDPM, they were 5.1 ± 3.8 ng/kg and 33.3%, respectively. The average level of AFM1 in all samples was lower than the EU and Iranian national standards (25 ng/kg). AFM1 intake through IDPM consumption by Iranian infants less than one-year-old was lower than the allowable level, but the hazard quotient for infants less than 6 months was higher than the allowable level. Although the concentration of AFM1 in IDPM consumed in Iran is not hazardous, since Iranian infants may be exposed to AFM1 through other sources, including baby food, breast milk, raw and pasteurized milk, continuous monitoring of IDMP quality in Iranian markets should be taken.

Levels of Aflatoxin M1 in Breast Milk of Lactating Mothers in Monterrey, Mexico: Exposure and Health Risk Assessment of Newborns.

The present study aimed to determine the presence of the aflatoxin M1 (AFM1) in breast milk samples from 123 nursing women and the degree of exposure of infants to this toxin, in the metropolitan area of Monterrey, Nuevo Leon state (northeast Mexico). Upon analysis, 100% of the samples were found to be contaminated with the toxin at an average concentration of 17.04 ng/L, with a range of 5.00 to 66.23 ng/L. A total of 13.01% of the breast milk samples exceeded the regulatory limit of 25 ng/L for AFM1 concentration, set by the European Union. The estimated daily intake for AFM1 and the carcinogenic risk index were also determined in the 0- to 6-, 7- to 12-, 13- to 24-, and 25- to 36-month-old age groups. The AFM1 intake through breast milk ranged from 1.09 to 20.17 ng/kg weight/day, and was higher than the tolerable daily intake, indicating a carcinogenic risk for infants in the age groups of 0- to 24-months old. This evidence demonstrates a susceptibility of breast milk to AFM1 contamination that may suggest a carcinogenic risk for the breastfed infants in Monterrey city, Nuevo Leon state, and the need to control the presence of aflatoxins in foods eaten by nursing mothers.

The Occurrence and Risk Assessment of Aflatoxin M1 in Yoghurt Samples from Hamadan, Iran

Background: Aflatoxin M1 (AFM1) is a hepatocarcinogenic and hydroxylated metabolite of aflatoxin B1, detected in milk and milk products. Objectives: The aim of our research was to determine the incidence and risk assessment of AFM1 through the consumption of yoghurt in Hamadan province of Iran. Methods: Fifty yoghurt samples were gathered from various areas of Hamadan province, Iran and tested for AFM1 by ELISA technique. The estimated daily intake (EDI) and the liver cancer incidence of aflatoxin M1 were determined. Results: AFM1 was detected in 43 (86%) samples, (mean: 28.56 ng/kg; range: <5-98.65 ng/kg). The level of AFM1 in 9 (18%) samples was above the maximum tolerance limit (50 ng/kg). The AFM1 intake through yoghurt consumption in various population groups ranged from 0.016 to 0.032 ng/kg bw/day in mean consumers and 0.019 to 0.046 ng/kg bw/day in high consumers. Conclusion: The AFM1 intake through yoghurt contributed a slight part from the overall incidence of liver cancer in the Iranian population. From the findings of the current study, it can be derived that although the high percentage of yoghurt samples in Iran proved to be contaminated with AFM1 contents, did not show a public health concern considering the European Commission (EC) and the Institute of Standards and Industrial Research of Iran (ISIRI) maximum limits.

Aflatoxin M1 Contamination in Raw Cow Milk of Dairy Farms in Behbahan area, Khuzestan province, Iran

Introduction: Mycotoxins are among the environmental chemical agents with toxic effects on animals and humans. Aflatoxins (AF) are a major group of mycotoxins produced following the growth of some species of Aspergillus flavus and Aspergillus parasiticus, particularly in agricultural products. Aflatoxin M1 (AFM1) is secreted to milk and remains there since the process of pasteurization and sterilization has little effect on this metabolite. The aim of this study was to investigate the natural occurrence of AFM1 in raw milk in Behbahan area, which is an area of high-density livestock production in southwest of Iran. The other aim was to compare these obtained levels with the accepted level recommended by the European community (50 ng/L). Methods: In the present study, 90 samples of raw milk were collected from five dairy farms in Behbahan region, Khuzestan province, Iran. The AFM1 was analyzed using the Enzyme Linked Immune Sorbent Assay (ELISA) method by ELISA kits from Euro Clone, Italy. Data were analyzed by ANOVA and the results were reported as mean ± SE. The significant level was set at p ≤ 0.05. Results: The concentration of aflatoxin M1 in (15)16.54% of raw milk was higher than maximum tolerance limit accepted by European Union /Codex Alimentarius Commission (50 ng/l). The results of this study showed that all 90 investigated raw milk samples were contaminated with AFM1 (100%). Conclusion: A significant difference was observed between concentration means of AFM1 in various studied farms at P < 0.05. Consequently, high occurrence of AFM1 in raw milk samples was considered as a possible hazard for human health. However, further longitudinal studies are required on milk samples and other dairy products in Behbahan as well as other regions of Iran to estimate the representative intake of AFM1 in Iran.

Risk associated with the intake of aflatoxin M1 from milk in Iran

Aflatoxin M1 is an oxidative metabolite of aflatoxin B1 formed in liver and excreted into milk, urine and faeces of dairy cattle and other mammalian species. The International Agency for Research on Cancer classified aflatoxin M1 in Group 2B because of its potential to get bioactivated to a mutagen analogous to aflatoxin B1. Risk assessments are undertaken to guide food regulators and scientists in risk management processes, such as the legislative levels or guideline targets for mycotoxins in food supplies. Using existing international resources for hazard data and local exposure data, and based on cancer potency as the endpoint, the risk of exposure to aflatoxin M1 in milk for the Iranian population was calculated considering various scenarios. During 2014-2015, 518 samples were collected from the market and tested for aflatoxin M1 contamination by HPLC-FLD. The most probable scenarios calculated as mean occurrence multiplied by the mean consumption in consumers of milk, and for maximum level allowed for aflatoxin M1 in milk with 99 percentiles of milk consumption showed the risk of 0.08 and 0.72 additional liver cancer cases per year for the Iranian population, respectively. Thus, our study reveals a low risk and that the current maximum limit of 100 ng/l for aflatoxin M1 in milk, heat treated milk and flavoured milk is sustainable.

Exposure to Aflatoxin M1 through Milk Consumption in Tehran Population, Iran.

Milk would be contaminated with Aflatoxin M1 (AFM1), if it was obtained from lactating animal which fed with feedstuffs containing Aflatoxin B1 (AFB1). AFM1 is classified as group 2B, possibly carcinogenic to humans and its exposure to AFM1 through milk consumption is a public concern. The purpose of this study was to determine the AFM1 exposure through liquid milk consumption for adult consumers in Tehran. Forty-five samples including raw, pasteurized, and UHT milk samples were collected from markets in different cities of Tehran province in January and February 2017. The AFM1 was determined by HPLC method after immunoaffinity column clean up. Also, the milk intake was calculated using household budget survey. Finally, the daily intake of AFM1 through milk consumption was estimated using a deterministic approach. From total 45 samples, AFM1 was detected in 36 (80%) samples, although none of the analyzed samples were exceeded Iran legal limit of 0.1 µg/kg. On the basis of the average milk intake, the mean daily exposure to AFM1 was estimated between 0.03 ng/ Kg BW per day (lower bound estimate) and 0.06 ng/ Kg BW per day (upper bound estimate) and the 95th percentile daily exposure was calculated at 0.14 ng/ Kg BW per day. According to these values, it should be expected that the adults of Tehran population are not exposed to a significant risk of Hepatocarcinoma associated with AFM1 intake through milk consumption.

Food surveillance in the Basque Country (Spain). II. Estimation of the dietary intake of organochlorine pesticides, heavy metals, arsenic, aflatoxin M1, iron and zinc through the Total Diet Study, 1990/91

Total diet samples purchased at monthly intervals between March 1990 and December 1991 were analysed for different contaminants and nutrients. Each total diet sample included 91 food items which were combined after preparation and/or cooking into 16 groups of similar foods. The 'market basket' was based on a food survey which referred to the adult population (25-60 years) carried out in the Basque Country between 1988 and 1990. The dietary intakes (micrograms/day) of lead (43), cadmium (11), mercury (18), arsenic (291), hexachlorobenzene (HCB) (0.2), alpha-hexachlorocyclohexane (alpha-HCH) ( < 0.1), beta-hexachlorocyclohexane (beta-HCH) (0.1), gamma-hexachlorocyclohexane (gamma-HCH) (2.9), dichlorodiphenyltrichloroethane (DDT) (0.3), dichlorodiphenyldichloroethene (DDE) (0.9), dichlorodiphenyldichloroethane (DDD)(0.2), dieldrin (0.5), heptachlor epoxide ( < 0.1), alpha-endosulphan (0.1) and beta-endosulphan (0.1) were all well below the respective Acceptable Daily Intakes or Provisional Tolerable Weekly Intakes. However, arsenic intake was much higher than that estimated in other countries and gamma-HCH was detected in anomalously high levels in the bread group. Dietary intakes of delta-hexachlorocyclohexane (delta-HCH), aldrin, endrin, heptachlor and methoxychlor were not calculated because no residues were detected in any of the samples. Aflatoxin M1 intake was not estimated owing to the low levels detected. Finally, zinc intakes (11.6 mg/day) were below the recommended dietary allowances for Spain and the same was true for iron (11.3 mg/day), but only for females.