Aeruginosa Strains Research Articles

The Antibiotic Resistance Patterns of Pseudomonas Aeruginosa Strains Isolated from Microbiological Specimens Experiences of A Single Center on the Pseudomonas Aeruginosa Strains

Purpose To determine the antibiotic resistance rates of Pseudomonas aeruginosa strains isolated from samples collected by the microbiology laboratory of a single center for four years. Methods The samples of wound, blood, tracheal aspirate, abscess, vagina, cerebrospinal fluid, sputum, and urine culture isolated from 789 patients who were hospitalized in the service, intensive care and outpatient services between 2017-2021 were evaluated retrospectively for Pseudomonas species. Results Most of culture were urine (42.7%) and sputum cultures (20.4%). Most patients applied to the service were from department of chest diseases (38.6%) or from department of urology (14.3%) or palliative care unit (12.5%). The highest resistances were against cefuroxime, levofloxacin and netilmicin; lowest resistance was against amikacin. The aztreonam, cefepime and gentamicin resistances were significantly reduced by years (P=0.0321, 0.0038 and 0.0004, respectively) while colistin and levofloxacin resistance considerably increased (P<0.0001 and P=0.0407, respectively). Significant decreases were observed in resistance against cefepime, ceftazidime and ciprofloxacin over years (P=0.0321, 0.0038 and 0.0004, respectively). A significant decrease in resistance of strains isolated from urine culture was only observed forcefepime over years (P=0.0003). The resistance of strains isolated from cultures of sputum, urine and respiratory secretions against levofloxacin significantly increased in 2019 while those of wound culture increased in 2020 (P=0.0145). Conclusion Alterations in the antibiotic resistance profile were detected in patients over years due to frequently varied use of antimicrobials. To select effective treatment protocols, it is necessary to regularly determine the microorganisms and their antibiotic resistance for each hospital.

Remediation of Crude Oil Contaminated Pit Water Using Pseudomonas Aeruginosa Strains

Remediation of Crude Oil Contaminated Pit Water Using Pseudomonas Aeruginosa Strains

Investigation of the Prevalence of CTX-M-1 Beta-Lactamase Gene in Pseudomonas Aeruginosa Strains Isolated From Urinary Tract Infections in Zanjan Hospitals, Iran

Investigation of the Prevalence of CTX-M-1 Beta-Lactamase Gene in Pseudomonas Aeruginosa Strains Isolated From Urinary Tract Infections in Zanjan Hospitals, Iran

Evaluation of Tobramycin and Ciprofloxacin as a Synergistic Combination Against Hypermutable Pseudomonas Aeruginosa Strains via Mechanism-Based Modelling.

Hypermutable Pseudomonas aeruginosa strains have a greatly increased mutation rate and are prevalent in chronic respiratory infections. Initially, we systematically evaluated the time-course of total and resistant populations of hypermutable (PAO∆mutS) and non-hypermutable (PAO1) P. aeruginosa strains in 48-h static concentration time-kill studies with two inocula. Both strains were exposed to clinically relevant concentrations of important antibiotics (aztreonam, ceftazidime, imipenem, meropenem, tobramycin, and ciprofloxacin) in monotherapy. The combination of tobramycin and ciprofloxacin was subsequently assessed in 48-h static concentration time-kill studies against PAO1, PAO∆mutS, and two hypermutable clinical P. aeruginosa strains. Mechanism-based mathematical modelling was conducted to describe the time-course of total and resistant bacteria for all four strains exposed to the combination. With all monotherapies, bacterial regrowth and resistant populations were overall more pronounced for PAO∆mutS compared to PAO1. The combination of tobramycin and ciprofloxacin was synergistic, with up to 106.1 colony forming units (CFU)/mL more bacterial killing than the most active monotherapy for all strains, and largely suppressed less-susceptible populations. This work indicates that monotherapies against hypermutable P. aeruginosa strains are not a viable option. Tobramycin with ciprofloxacin was identified as a promising and tangible option to combat hypermutable P. aeruginosa strains.

Virulence Genes in Pseudomonas Aeruginosa Strains Isolated at Suez Canal University Hospitals with Respect to the Site of Infection and Antimicrobial Resistance

Virulence Genes in Pseudomonas Aeruginosa Strains Isolated at Suez Canal University Hospitals with Respect to the Site of Infection and Antimicrobial Resistance

Genotyping of Pseudomonas Aeruginosa Strains As A Multidrug Resistant (MDR) Bacterium And Evaluating The Prevalence of Esbls and Some Virulence Factors Encoding Genes By PFGE and ERIC-PCR Methods.

Pseudomonas aeruginosa is an important multi-drug resistant (MDR) opportunistic bacterium. 102 strains of Pseudomonas aeruginosa equally isolated from human and cow milk were subjected to Multiplex-PCR for detection of ESBLs and exoenzymes of U, T, S, OprI, and OprL, Integrons class A encoding genes and genotyping by the ERIC-PCR and PFGE methods. The disc diffusion and E-test based on CLSI (Clinical and Laboratory Standards Institute) were performed to identify the antibiotics’ resistant strains. Exotoxin A encoding gene was detected in more than 90% of the studied strains, exoenzyme S prevalence in isolated samples from animal (cow milk) was negative and the frequency of Exo Y, Exo T, and Exo U were 25%, 68.6%, and 68.6%, respectively. The frequency of VEB and GES encoding genes in human strains were detected as 3.9% and 0 by Multiplex-PCR, respectively. The highest resistance was seen to Ampicillin and Cefepime (100%) while the lowest was observed to Amikacin (80.3%). E-Test results on human and animal strains showed complete resistance to Meropenem and Ampicillin, respectively. Dendrogram of ERIC-PCR method on human isolated samples revealed 22 different groups. Frequency of Integron I encoding gene was detected as 21.5% and 1.96% in human and animal strains, respectively. In general, the present study showed the high value of genetic diversity among isolates from animal and human samples with different progenitors, but the clones classified in one cluster revealed the same source of infection.

Determining the Prevalence of qnrB Gene in Pseudomonas Aeruginosa Strains Isolated from Clinical Centers in Tehran in 1394

Determining the Prevalence of qnrB Gene in Pseudomonas Aeruginosa Strains Isolated from Clinical Centers in Tehran in 1394

A Clinical Debate concerning Aminoglycoside Resistance Genes among Pseudomonas Aeruginosa Strains

A Clinical Debate concerning Aminoglycoside Resistance Genes among Pseudomonas Aeruginosa Strains

Molecular Identification and Virulence Factors of Pseudomonas Aeruginosa Strains Isolated from Animal Products

Pseudomonas aeruginosa is an opportunistic pathogen which is characterized by its capacity to develop resistance and virulence factors Despite their knowledge these resistance and virulence factors are not more characterized at level of strains from local food This study aimed to detect the presence of various virulence factors and metallo beta lactamase producing P aeruginosa from animal products One hundred strains of Pseudomonas aeruginosa were isolated from bovine meat fresh fish and smoked fish API NE methods IPM EDTA disk method and polymerase chain reaction PCR using the rpoB lasB exoS algD and plcH gene were performed The rpoB gene confirmed of presumptive strains as Pseudomonas aeruginosa All of virulence genes studied were detected in decreasing importance order lasB exoS algD and plcH p lt A percentage of of animal strains were P aeruginosa producing MBLs The simultaneous determination of virulence factors and metallo beta lactamase is of interest for the efficacy of surveillance of infections associated with P aeruginosa

A Comparison of Multidrug Resistance Rates of Pseudomonas Aeruginosa Strains in Burn Patients in Iran in 2006 and 2015

A Comparison of Multidrug Resistance Rates of Pseudomonas Aeruginosa Strains in Burn Patients in Iran in 2006 and 2015

Association of the Exotoxin A and Exoenzyme S with Antimicrobial Resistance in Pseudomonas Aeruginosa Strains.

Pseudomonas aeruginosa is a gram-negative and opportunistic bacterium that causes opportunistic infections in immunosuppressed patients. The main purpose of this study was to investigate the association between exotoxin A and exoenzyme S production with antibiotic resistance patterns. The toxA and exoS genes were detected in 102 clinical isolates by PCR. Antibiotic susceptibility tests were performed by disk diffusion method (Kirby Bauer). The Chi-square and Fisher's test were used for evaluation of the association between "toxins and infections source" and "toxins and antibiotic resistance" respectively. Frequency of toxA+ and exoS+ strains was 81% and 61%, respectively. The association between drug resistance and toxA+ genotype was significant for all antibiotics tested (P < 0.05) except aztreonam; however, no significant association was observed between drug resistance and exoS gene (P > 0.05). High frequency of toxA+ resistant strains isolated from inpatients and significant association between the toxin and drug resistance in more antibiotics, reinforces possible role of exotoxin A as an extracellular protein in the regulation of drug resistance genes. The results may be further verified by Southern blot analysis of toxA and exoS gene expression and elucidation of the mechanism of antibiotic resistance.

P870 GES-1 and TEM-1-like β-lactamases of Pseudomonas aerug-inosa strains isolated from patients in two Warsaw hospitals

P870 GES-1 and TEM-1-like β-lactamases of Pseudomonas aerug-inosa strains isolated from patients in two Warsaw hospitals

A Plasmidic Class 1 Integron from Five Pseudomonas aeruginosa Clinical Strains Harbored aacA4 and Nonsense-mutated cmlA1 Gene Cassettes

Five strains of multidrug-resistant Pseudomonas aeruginosa (P. aeru ginosa )w ere isolate df rom inpatients at a local hospital in China. The most frequent resistance was to cefoperazone, ciprofloxacin, ceftriaxone, cefotaxime, gentamicin, piperacillin, trimethoprim sulfamethoxazole ,a nd tobramycin. These strains were found to contain the class 1 integron, in which the 2360-bp gene cassettes were flanked by 5 � -a nd 3 � -conserved segments. Sequence analysis revealed that the gene cassettes contained aacA4 and cmlA1 genes; however, the latter gene had a nonsense mutation resulting in the production of a truncated protein. To the best of our knowledge, this is the first report of a nonsense mutation in the cmlA1 gene. Moreover, the P. aeru ginosa strains showed identical profiles in pulsed-field gel electrophoresis, suggesting that they were derived from the same clone. These results emphasize the importance of controlling the spread of multidrug-resistant pathogens in hospitals.