Aerobic Reactor Research Articles

Color removal in acidogenic reactor followed by aerobic granular sludge reactor: operational and microbiological aspects

This work investigated the operational and microbiological aspects of the decolorization of the azo dye Reactive Black 5 in acidogenic reactors followed by aerobic granular sludge (AGS) reactors, evaluating the effect of the acidogenic hydraulic retention time (HRT) (3, 2, and 1 h), effluent recirculation in the AGS reactor (50 mL·min-1), dye concentration (50 and 100 mg·L-1), and the redox mediator sodium anthraquinone-2-disulfonate (AQS) (50 μM). The acidogenic reactors were mainly responsible for the dye decolorization, with AQS significantly improving its efficiency and enabling the use of a shorter HRT (2 h). The recirculation effect was not so evident, probably masked by the adaptation of the acidogenic microbiota. Increasing the dye concentration did not affect the total decolorization, but reduced nitrogen removal in the AGS reactors. Furthermore, the dye and its byproducts may have negatively affected the long-term AGS stability. While the acidogenic microbiota maintained its diversity, the AGS tended to become more specialist. However, in both, some abundant genera that may have acted in reducing the dye were found, such as Clostridium_sensu_stricto_1 and Raoutella in the acidogenic sludge and Dechloromonas and Defluviicoccus in the AGS.

Influence of oxygen transfer performance on nitrogen metabolism and microbial community structure in an aerobic fluidized bed biofilm reactor under different aeration aperture

Influence of oxygen transfer performance on nitrogen metabolism and microbial community structure in an aerobic fluidized bed biofilm reactor under different aeration aperture

Urine luck: Environmental assessment of yellow water management in buildings for urban agriculture

The increasing global demand for agricultural production poses challenges to maintain the needs for critical fertilizers such as nitrogen. This study explores the potential of human urine as a source of renewable nitrogen for fertilizer production. Through a life cycle assessment, three different urine management strategies were compared: (S1) an artificial wetland, (S2) an on-site lab-scale aerobic reactor for nitrogen recovery, and (S3) a centralized wastewater treatment plant. While scenario S2 had the highest impacts in 6 out of 8 categories, an advantage in marine eutrophication was identified. S2 showed high energy demand (750 kg MJ-eq) and ecotoxicity (602 kg 1.4-DCB-eq.) mainly due to energy requirements. Nitrogen production exceeded 2.3 times the yearly nitrogen demands of the building tomato production. Upscaling S2 reduces impacts up to 2 times, lowering the payback time from 29 to 13 years. Therefore, implementing large-scale nitrogen recovery systems in cities is encouraged.

Remoción de cromo (VI) de agua residual de curtiembre empleando biomasa orgánica vegetal en un reactor biológico secuencial aerobio

[Introduction]: In the removal of chromium (VI) from tannery wastewater, physicochemical methods are the most used. However, these require high operating costs, and worse still, generate secondary pollutants that again require complementary treatments for their elimination. Given this, the applicability of plant organic biomass (VOB) emerges as an environmental alternative based on the adsorption of chromium (VI), the simplicity of obtaining it, the reduction as waste and, above all, it does not generate secondary pollutants. [Objective]: Use VOB waste in an aerobic sequential biological reactor (SBR) for the removal of chromium (VI) in the treatment of tannery wastewater. [Methodology]: 3 aerobic SBR systems were implemented; 2 controls (SBR1 and SBR2) and 1 study subject (SBR+VOB). Each system was fed with tannery wastewater (18 L) and connected to an aeration system (12.7 L/min). The bacterial inoculum, a fundamental part of an SBR system, was obtained from the selective culture of chromium (VI) reducing bacteria (RBCrVI) taken from the wastewater under study, and integrated into SBR2 and SBR+VOB at 0.6 g/L. The VOB was obtained from Stenotaphrum secundatum residues, fragmented, sieved, sterilized and integrated into the SBR+VOB at 1.5 g/L. [Results]: The SBR+VOB system achieved the highest percentage of chromium (VI) removal (93 %), followed by SBR2 (80.1 %), and finally SBR1 (2.8 %). With respect to COD and TSS, the statistical test (Tukey) showed that there was no significant difference between the system under study and the controls, recording COD removal percentages ranging from 87.8 % to 88.4 %, and TSS ranging from 63.8 % to 69.2 %. [Conclusions]: The viability of using VOB waste to remove chromium (VI) in the treatment of tannery wastewater has been evidenced. The results of this research create a promising panorama to mitigate the effects derived from inadequate management of tannery wastewater and organic solid waste.

Evaluation of Integrated Anaerobic/Aerobic Conditions for Treating Dye-Rich Synthetic and Real Textile Wastewater Using a Soda Lake Derived Alkaliphilic Microbial Consortia

Textile industry wastewater (WW) has intense color, high chemical oxygen demand (COD), pH, and salinity, making it challenging for conventional treatment. Soda lakes, with high alkalinity and salinity, host diverse microbes capable of textile dye degradation. This study evaluated anaerobic/aerobic reactors using alkaliphilic microbial consortia from Lake Chitu, an Ethiopian soda lake, for treating synthetic and real textile WW. The experimental setup consisted of a first-stage anaerobic reactor followed by a second-stage aerobic reactor, operating continuously with a predetermined flow rate and hydraulic residence time. After evaluating synthetic WW, real textile WW was collected in two batches (rounds I and II). The treatment setup removed 99% of the dye color for synthetic WW, 98% for round I, and 96% for round II. COD removal was 87% for synthetic WW, 86% for round I, and 93.37% for round II. TKN removal reached 90% for synthetic WW, 91% for round I, and 96% for round II at a steady state. Residual COD and TKN values met the final effluent discharge standards. GC–MS and IR analyses revealed that dyes were broken down into intermediate organic compounds under anaerobic conditions and further degraded into smaller molecules under aerobic conditions. This integrated reactor approach effectively removes dyes and enhances COD and TKN removal. The study’s novelty lies in evaluating both synthetic and real textile WW using integrated reactors under alkaline conditions in a continuous process, inoculating alkaliphilic consortia, without pre-enrichment or external nutrient addition to real WW. The study provides insights into the effectiveness of alkaliphilic microbial consortia derived from soda lakes for treating textile WW using integrated reactor conditions. Reactor microbiome characterization is needed to further explore microbial diversity and community structure.

Ammonia oxidizing bacteria (AOB) denitrification and bacterial denitrification as the main culprit of high N2O emission in SBR with low C/N ratio wastewater

A large amount of greenhouse gas nitrous oxide (N2O) will be produced during the biological nitrogen removal process for organic wastewater of low C/N ratio. One of the effective methods to solve this problem is to incorporate inexpensive carbon source. In this study, raw wastewater (RW) from pig farm, that was not anaerobically digested, was utilized as exogenous carbon in both A/O and SBR aerobic reactor to treat liquid digestate with high ammonia nitrogen and low C/N ratio. The results showed that N2O emission in SBR was higher than that of A/O process under the same nitrogen load. The N2O conversion in the biological nitrogen removal process was investigated by the strategy of integrating stable isotope method and metagenomics. The δO18-N2O, δN15-N2O, and SP values of the SBR were closer to the denitrification values of Ammonia-Oxidizing Bacteria (AOB) than those of A/O. The abundance of AOB in the SBR reactor was higher than that in the A/O reactor, while the abundance of denitrifying bacteria was lower. The amoA/B/C gene abundance in the SBR was greater than that in the A/O, and the NOS gene abundance was the opposite. The results indicated that both AOB denitrification and bacterial denitrification led to the increase of N2O emissions of the SBR.

Life Cycle Assessment of Industrial Wastewater Treatment Trains

AbstractAlternative technologies to granular activated carbon (GAC) are of interest to improve the sustainability and reduce the cost of munitions wastewater treatment. Research efforts have highlighted GAC alternatives, yet few reports of environmental and economic impacts associated with these technologies are available. Herein, a life cycle assessment (LCA) aids in assessment of environmental impacts associated with six munitions wastewater treatment configurations—specifically GAC, compared to five configurations that include combinations of ion exchange (IX), reverse osmosis (RO), aerobic granular reactors (AGR), UV/H2O2, and ozone technologies. The LCA compares environmental impacts generated by treating 1 m3 of munitions wastewater, impacts by life cycle stage, and effects of IX, RO, and GAC replacement frequency. Results show that IX resin pairs with AGR (for flow‐through treatment) and ozone (for IX regenerant treatment) generated 22 ± 18% less impact than GAC in nine of ten environmental impact categories during production, transportation, and disposal. Treatment trains with ozone or AGR produce 35% less environmental impact than those with UV/H2O2. Production and use stages generate more environmental impacts than transportation and disposal stages for most treatment technologies. This LCA provides insights into the sustainability of six munition wastewater treatment technologies and identifies areas where treatment sustainability can be improved.

Nitrogen removal pathways and microbial community structure in the aerobic granular sludge reactor with co-existence of granules and flocs

Granules and floc in the aerobic granular sludge (AGS) reactor undertook different roles in the nitrogen removal process and displayed significant disparities in microbial communities. This study quantified the contribution of different nitrogen removal pathways and analyzed the nitrogen removal mechanisms inside granules and flocs by metagenomics sequencing. The results showed that the AGS reactor performed excellent nitrogen removal efficiency and the removal efficiencies of NH4+-N and total nitrogen were more than 90.0 % and 60.0 %, respectively, with the main nitrogen form of NO2--N in the effluent. Batch experiments indicated that the nitrogen removal pathway was principally shortcut nitrification and denitrification (SCND), accounting for 51.6 %, followed by anaerobic ammonium oxidation (Anammox) accounting for 26.1 % and the remaining 22.3 % was simultaneous nitrification and denitrification (SND). The metagenomic sequencing results pointed out that the abundance of SCND-related genes in the granules was 0.18 %, and the abundance of Anammox-related genes was 0.02 %, which indicated that the granules mainly reduced nitrogen via SCND, and minor nitrogen was removed through Anammox. Inside the flocs, nitrogen was mainly reduced via the unconventional SND pathway (HAND). The in-depth analysis of the nitrogen removal process in the reactor with granules and flocs provides an important reference for further exploring the nitrogen removal mechanism, and supplies foundation for expanding the application of AGS.

First evidence for temperature’s influence on the enrichment, assembly, and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities

Polyhydroxyalkanoates (PHA) are popular biopolymers due to their potential use as biodegradable thermoplastics. In this study, three aerobic sequencing batch reactors were operated identically except for their temperatures, which were set at 15 °C, 35 °C, and 48 °C. The reactors were subjected to a feast–famine feeding regime, where carbon sources are supplied intermittently, to enrich PHA-accumulating microbial consortia. The biomass was sampled for 16S rRNA gene amplicon sequencing of both DNA (during the enrichment phase) and cDNA (during the enrichment and accumulation phases). All temperatures yielded highly enriched PHA-accumulating consortia. Thermophilic communities were significantly less diverse than those at low or mesophilic temperatures. In particular, Thauera was highly adaptable, abundant, and active at all temperatures. Low temperatures resulted in reduced PHA production rates and yields. Analysis of the microbial community revealed a collapse of community diversity during low-temperature PHA accumulation, suggesting that the substrate dosing strategy was unsuccessful at low temperatures. This points to future possibilities for optimizing low-temperature PHA accumulation.

Influence of residual anaerobic granular sludge (AnGS) from anaerobically digested molasses wastewater in aerobic granular sludge reactor

This study investigated the impact of residual anaerobic granular sludge (AnGS) from anaerobic digesters treating molasses wastewater on ammonium reduction in a downstream aerobic granular sludge (AGS) reactor. Two conditions were tested: raw (high AnGS concentration) and settled (low AnGS concentration) anaerobically digested molasses wastewaters were fed into the AGS reactor. With the introduction of raw wastewater, enhanced nitrite accumulation at 30 % and improved total inorganic nitrogen (TIN) removal at 11 % were observed compared to 1 % nitrite accumulation and 8 % TIN removal with the introduction of settled wastewater. However, AnGS adversely affected other aspects of reactor performance, increasing effluent solid content and decreasing soluble chemical oxygen demand removal efficiency from 20 % in the low AnGS condition to 11 % in the high AnGS condition. Despite the observed retention of AnGS in the reactor, no significant bioaugmentation effects on the microbial community of the AGS were observed. Aerobic granular sludge was consistently observed in both conditions. The study suggests that AnGS may act as a nucleus for granule formation, helping to maintain granule stability in a disturbed environment. This study offers a systematic understanding of the impact of AnGS on subsequent nitrogen removal process using AGS, aiding in the decision making in the treatment of high solid anaerobic digestate.

A novel three-dimensional biofilm-electrode/anoxic-oxic process for nitrogen and phosphorus removal: Performance, mechanism and microbial community

Traditional anoxic-oxic (AO) process cannot meet increasingly high pollutants emission standards in China. In order to improve removal efficiencies of the pollutants and the electron transfer rate, graphite-graphite electrodes and iron carbon fillers were added into AO reactor to form three-dimensional biofilm electrode/anoxic aerobic reactor (3DBER/AO). Removal efficiencies of the pollutants, sludge properties and microbial community structure were investigated under different current intensities. The results showed that microbial activity and removal efficiencies of the pollutants were the maximum, and mass percentage of Fe reached the highest value of 5.11 % under the current of 40 mA. Excessive or insufficient current intensity could lead to a decrease in sludge activity and removal efficiencies of the pollutants. The removal of nitrogen mainly depended on the nitrification and denitrification of microorganisms, and dissolved phosphorus was removed through chemical precipitation. Current intensity affected microbial communities and biosynthesis. AKYH767 and Saccharmonadales were dominant microorganisms in the cathode and anode sludge, respectively, under the current of 40 mA. However, Azospira and Dechlorobacter were dominant microorganisms in the cathode sludge, and Hydrogenophaga was dominant microorganisms in the anode sludge under the current of 60 mA. These microorganisms were closely related to the removal of organic matter, nitrogen and phosphorus. Therefore, microbial community structure and removal efficiencies of the pollutants could be regulated by controlling the current intensity in the 3DBER/AO process.

Elucidating the role of organic loading rate on the performance and microbial dynamics of a novel continuous-flow aerobic granular sludge reactor

Aerobic granular sludge technology operated in continuous-flow reactors enables the upgrading of existing conventional wastewater treatment plants. The viability of applying this technology at full-scale increases with the simplicity of the bioreactor design. Hence, in contrast to previous research carried out in complex configurations, this study is based on evaluating the capacity of a novel single-chamber continuous-flow bioreactor to operate at different loads found in domestic sewage, highlighting that this configuration combines and joins the advantages of activated sludge and granular sludge technologies. At organic loading rates ranging from 0.45 to 1.85 kg COD•m−3•d−1, the reactors achieved high performance, removing >80 % of the chemical oxygen demand, regardless influent characteristics. The trend of ammonium removal rates was risen over operational time. Moreover, the granular size and biomass concentration increased with the organic loading rate. Changes in the influent did not compromise the structure of the granules. Microbiota studies showed a high diversity in communities at lower organic loading rates, whereas a strong microbial competition was detected at high loading rates, linking with lower evenness. Phylotypes belonging to Hypocreales played a key role in mature granules.

Treatment of high ammonia anaerobically digested molasses wastewater using aerobic granular sludge reactor

This study addressed the treatment of high ammonia, low biodegradable chemical oxygen demand (bCOD) anaerobically digested molasses wastewater, utilizing an aerobic granular sludge (AGS) reactor. The AGS achieved 99 % ammonia removal regardless of the bCOD supplementation. By adding low ammonia (<60 mg/L), high bCOD raw molasses wastewater (before anaerobic digestion) as a carbon source, enhanced nitrogen removal, increasing from 10 % to 97 %, and improved sludge settleability via bio-induced calcite precipitation were observed. Functional genes prediction suggested two potential denitrification pathways, including heterotrophic denitrification by Paracoccus and Thauera, and autotrophic denitrification, specifically sulfide-oxidizing autotrophic denitrification by Thiobacillus. An increase in the relative abundance of microorganisms involved in heterotrophic denitrification was observed with the addition of high bCOD raw molasses wastewater. Consequently, incorporating raw molasses wastewater into the AGS presents a sustainable approach to achieve mixotrophic denitrification, maintain stable granular sludge and ensure stable treatment performance when treating anaerobically digested molasses wastewater.

Enhancement the wastewater treatment performance of multistage living machine by underwater lamp

Source separation and decentralized domestic wastewater treatment represent effective strategies to enhance sewage treatment performance and facilitate water reuse economically. The Living Machine (LM) system has gained widespread adoption for decentralized sewage treatment. While underwater light source has been demonstrated to enhance the treatment performance of open aerobic reactors in LM systems, its influence on the treatment efficiency of a fully multistage LM system remains underreported. In this study, an underwater lamp-added LM system (ULLM) with eight reactors was constructed and investigated. The introduction of underwater light source obviously improved the removal capacity of chemical oxygen demand (COD) and NH4+-N, which was 96.1% and 61.6%, respectively. The diversity of algae, zooplankton, and aquatic animals was notably higher in the light-treated reactors than in the control group (CK) without underwater light source, and substantial alteration in the microbial community of the light-treated reactors was observed compared with CK reactors. At the phylum level, Proteobacteria and Nitrospirae enriched in the underwater light-treated reactors, while Bacteroidetes and Actinobacteria exhibited a decrease after light exposure. At the genus level, Nitrospira and Rhodanobacter were enriched in the ULLM system. Importantly, the prevalence of these two dominant genera was sustained until the final operational stage, indicating their potential key roles in enhancing wastewater treatment performance. The addition of underwater light source proves to be an effective strategy for augmenting the treatment efficiency of the multistage living machine systems, resulting in substantial improvements in pollutant removal. These findings contribute valuable insights into optimizing LM systems for decentralized wastewater treatment.

Aerobic granular sludge sequencing batch reactor for high strength domestic wastewater treatment: Assessing kinetic models and microbial community dynamics

The study aims to treat high strength domestic wastewater (HSDW) and cultivate microbial granular sludge in a sequencing batch reactor (SBR). A bench-scale bioreactor was used for startup. Response surface methodology (RSM) was used to determine the ideal parameters for removing organic materials from HSDW. Organic loading rate (OLR) and cycle time were the independent variables taken into consideration, while the response variable was COD removal. Results from RSM revealed that the optimal conditions for achieving > 98 % COD and TP (>80) removal were a cycle length of 6 h and an OLR of 1.5 kgCOD/m3d. The Modified-Kincannon and the Grau second-order kinetic models were compared to the COD removal rate for the SBR system. The saturation rate constant (Ni) and maximal substrate removal rate (Vc) were proposed to be 6.8 g L−1 d−1 and 0.83 g L−1 d−1, respectively, based on the modified Stover-Kincannon model. The modified Stover-Kincannon (R2 = 0.93673) and Grau second order (R2 = 0.99219) models high correlation coefficient values show that the experimental results and the prediction for the microbial aerobic granular based SBR system accord well. It was discovered that the modified Stover-Kincannon model was less suitable than the Grau second order model. The system microbial community changed because of the granulation process, as the reactor design operation had a big impact on the community structure. Decreased filamentous bacteria favoured several taxa that have been found to exist in granular biomass that is appropriate for treating wastewater. This study sheds important light on the complex interactions that occur between microbial populations and operational variables all through the granulation process in SBR.

CFD-PBM simulations the effect of aeration rates on hydrodynamics characteristics in a gas-liquid-solid aerobic fluidized bed biofilm reactor

A CFD-PBM coupling model was developed to simulate and calculate multiphase flow parameters, flow morphology, and bubble diameter in the aerobic fluidized bed biofilm reactor (AFBBR) under different aeration rates. The simulated radial solid volume fraction values were generally in agreement (within ±15%) with the experimental values. The gas phase predominantly occupied the central area of the reactor, with three distinct velocity peaks observed in the liquid and solid phases. Higher aeration rates improve the mixing of multiple phases by augmenting fluidization velocity, gas-phase volume fraction, eddies size, and turbulence characteristics, thereby leading to a rise in the average size of bubbles from 1.54 mm to 2.03 mm. However, the proportion of small diameter bubbles (0.27–1.03 mm) decreased from 69.4% to 59.6%. These studies concluded that the multiphase flow parameters under an aeration rate of 5.77 m3/(h·m3) were more favorable for improving oxygen mass transfer efficiency and reducing energy consumption.

Partial nitrification and simultaneous denitrification in sequential anaerobic and aerobic reactors: performance and microbial community dynamics

ABSTRACT The removal of organic matter and nitrogen from domestic sewage was evaluated using a system composed of two sequential reactors: an anaerobic reactor (ANR) with suspended sludge and an aerobic (AER) reactor with suspended and adhered sludge to polyurethane foams. Nitrogen removal consisted of AER operating at low dissolved oxygen (DO) concentrations; this favoured the simultaneous nitrification and denitrification (SND) process. The concentration of COD and N were 440 mgO2.L−1 and 37 mgTN.L−1, respectively. The operation was divided into three phases (P), lasting 51, 53, and 46 days, respectively. The initial DO concentrations applied in the AER were: 3.0 (PI) and 1.5 mg.L−1 (PII and PIII). In PIII, the AER effluent was recirculated to the ANR at a ratio of 0.25. Kinetic assays were performed to determine the nitrification and denitrification rates of the biomasses (ANR and AER in PIII). Changes in the microbial community were evaluated throughout phases PI to PIII by massive sequencing. In PIII, the best results obtained for chemical oxygen demand (COD) and total nitrogen (TN-N) removal efficiencies, were close to 94% and 65%, respectively. Under these conditions, system effluent concentrations below 30 mg COD.L−1 and 15 mg TN-N.L−1 were verified. The nitritation and nitration rates were 10.5 and 6.5 mg N.g VSS−1.h−1, while the denitrification via nitrite and nitrate were 6.8 and 5.8 mg N.g VSS−1.h−1, respectively. A mixotrophic community was prevalent, with Rhodococcus, Nitrosomonas, Pseudomnas, and Porphyromonas being dominant or co-dominant in most of the samples, confirming the SND process in the AER sludge.

Taxonomic Identification of Microbiota from Enrichment and Isolation of Phenanthrene-Degrading Bacteria in Aerobic Reactors

Taxonomic Identification of Microbiota from Enrichment and Isolation of Phenanthrene-Degrading Bacteria in Aerobic Reactors

Kinetic Characteristics of Thermophilic Aerobic Membrane Reactor (TAMR) for Iraqi Municipal Wastewater

Kinetic Characteristics of Thermophilic Aerobic Membrane Reactor (TAMR) for Iraqi Municipal Wastewater

Nitrate increases the capacity of an aerobic moving-bed biofilm reactor (MBBR) for winery wastewater treatment.

We used bench-scale tests and mathematical modeling to explore chemical oxygen demand (COD) removal rates in a moving-bed biofilm reactor (MBBR) for winery wastewater treatment, using either urea or nitrate as a nitrogen source. With urea addition, the COD removal fluxes ranged from 34 to 45 gCOD/m2-d. However, when nitrate was added, fluxes increased up to 65 gCOD/m2-d, twice the amount reported for aerobic biofilms for winery wastewater treatment. A one-dimensional biofilm model, calibrated with data from respirometric tests, accurately captured the experimental results. Both experimental and modelling results suggest that nitrate significantly increased MBBR capacity by stimulating COD oxidation in the deeper, oxygen-limited regions of the biofilm. Our research suggests that the addition of nitrate, or other energetic and broadly used electron acceptors, may provide a cost-effective means of covering peak COD loads in biofilm processes for winery or another industrial wastewater treatment.