Aerobic Bacterial Isolates Research Articles

Recent Bacteriological Trends and Emerging Antimicrobial Resistance among Hospitalized Patients in a Tertiary Care Center, Central India

ABSTRACT Background: Emergence of antimicrobial resistance (AMR) is a major health concern worldwide. Knowledge about common hospital organisms and their antibiograms play a crucial role in minimized mortality caused by multidrug resistance organisms (MDROs). Aim: This study determines the bacteriological trends and antibiotic resistance patterns of various clinical samples among hospitalized patients. Methods: Various clinical specimens were received and processed for culture and identification as per standard operating procedures (SOPs). Antimicrobial susceptibility tests (ASTs) were performed as per standard guidelines. Results: The prevalence of aerobic bacterial isolates was 31.68%. The most common bacterial isolates were Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Klebsiella species, and Pseudomonas species. The majority of isolates were MDROs. Conclusion: Regular surveillance of MDRO helps in implementing better therapeutic strategies to reduce morbidity and mortality associated with them.

Prevalence and Antimicrobial Susceptibility Patterns of Wound and Pus Bacterial Pathogens at a Tertiary Care Hospital in Central Riyadh, Saudi Arabia

The long history and extensive use of antibiotics have caused resistant bacterial pathogens to emerge, increasing mortality and morbidity. The current study was designed to see the prevalence of aerobic bacterial isolates with their antimicrobial resistance pattern from out- and inpatients requested for wound or pus culture. Retrospective study conducted at a tertiary care hospital in central Riyadh from January 2023 to December 2023. Samples were collected and inoculated onto the appropriate media following standard guidelines. Bacterial pathogens were identified by the Vitek2 compact system. Antimicrobial susceptibility was tested using the Kirby–Bauer disk diffusion method as well as by MIC determination through the Vitek2 compact. A total of 1186 subjects were included in the study with a bacterial isolation rate of 691 (58.3%). Out of these, 155 positive cultures had incomplete information or anaerobic or fungal growth and were excluded from the study. With a slight female predominance (54.9%), the majority of subjects (72.2%) were outpatients, and over half of the isolates (55.2%) were Gram-positive. The most common isolate was Staphylococcus spp. (44.4%), followed by E. coli (13.6%) and P. aeruginosa (12.9%). The highest resistance was reported against penicillin followed by fusidic acid against Gram-positive bacteria. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in 40.5% of Staphylococcus aureus (S. aureus) isolates. Amikacin was the most susceptible antibiotic against all Gram-negative isolates. MDR Gram-negative bacteria accounted for 51.9% of wound infection isolates (95% CI: 45.95 to 58.33) while 6.3% (95% CI: 4.39 to 8.86) were XDR (nonsusceptibility to at least one agent in all but two or fewer antimicrobial categories). A high prevalence of bacterial isolates, with S. aureus as the predominant pathogen, showed high rates of multidrug resistance. This highlights the importance of monitoring antibiotic choices for prophylaxis and treatment in the study area.

Microbial responses to elevated temperature: Evaluating bentonite mineralogy and copper canister corrosion within the long-term stability of deep geological repositories of nuclear waste

Deep Geological Repositories (DGRs) consist of radioactive waste contained in corrosion-resistant canisters, surrounded by compacted bentonite clay, and buried few hundred meters in a stable geological formation. The effects of bentonite microbial communities on the long-term stability of the repository should be assessed. This study explores the impact of harsh conditions (60 °C, highly-compacted bentonite, low water activity), and acetate:lactate:sulfate addition, on the evolution of microbial communities, and their effect on the bentonite mineralogy, and corrosion of copper material under anoxic conditions. No bentonite illitization was observed in the treatments, confirming its mineralogical stability as an effective barrier for future DGR. Anoxic incubation at 60 °C reduced the microbial diversity, with Pseudomonas as the dominant genus. Culture-dependent methods showed survival and viability at 60 °C of moderate-thermophilic aerobic bacterial isolates (e.g., Aeribacillus). Despite the low presence of sulfate-reducing bacteria in the bentonite blocks, we proved their survival at 30 °C but not at 60 °C. Copper disk's surface remained visually unaltered. However, in the acetate:lactate:sulfate-treated samples, sulfide/sulfate signals were detected, along with microbial-related compounds. These findings offer new insights into the impact of high temperatures (60 °C) on the biogeochemical processes at the compacted bentonite/Cu canister interface post-repository closure.

Bacteriological Profile and Antibiogram of Aerobic Blood Culture Isolates among Suspected Blood Stream Infections: A Cross-sectional Study

Introduction: Bloodstream Infections (BSIs) are important causes of morbidity and mortality worldwide. Blood culture is the most important diagnostic tool for BSI. The epidemiology and outcome of BSI are constantly changing due to increasing antimicrobial resistance, changing patterns of antibiotic drug usage, and the increased use of transient or permanent medical devices. Thus, regular monitoring of the bacterial aetiology of BSI and their antibiograms is necessary. Aim: To determine the bacteriological profile and antibiotic susceptibility patterns among suspected BSI patients. Materials and Methods: A hospital-based cross-sectional study was carried out in the Department of Microbiology, Jawaharlal Nehru Institute of Medical Sciences (JNIMS), Imphal, Manipur, India, from September 2018 to August 2020. A total of 481 suspected BSI patients were included in the study. The blood culture samples were collected under aseptic conditions and cultured for seven days. The isolates were identified using conventional biochemical tests, and the antibiotic susceptibility pattern was determined by the Kirby-Bauer disk diffusion method and the epsilometer test (E-test). Methicillin resistance among the staphylococcal isolates was detected using the cefoxitin disk diffusion method. Data analysis was performed using EpiInfoTM version 7.0, and the Chi-square test was used as a statistical test. Results: A total of 150 (31.2%) blood samples were culture positive out of the 481 samples processed from suspected BSI patients. The predominant isolates were gram-positive, accounting for 113/150 (75.3%), while gram-negatives accounted for 37/150 (24.7%). Staphylococcus aureus, Pseudomonas aeruginosa, and Coagulase Negative Staphylococci (CoNS) were the primary pathogens isolated. Methicillin resistance was detected in 59 (54.6%) of the staphylococcal isolates {52 (54.7%) were Methicillin Resistant S. aureus (MRSA), and out of the 13 CoNS isolates, 7 (53.9%) were Methicillin Resistant CoNS (MRCoNS)}. The most susceptible drugs for gram-positives were vancomycin and linezolid, while aminoglycosides and imipenem were the most susceptible drugs for gram-negatives. Conclusion: The study highlighted the prevalence and pattern of aerobic bacterial isolates in BSI cases at JNIMS Hospital, as well as, the antibiotic susceptibility patterns of the isolates. Regular epidemiological studies of BSIs, regarding the pathogens and their antibiotic susceptibility patterns, are necessary to guide clinicians in choosing appropriate empirical therapy and to update the hospital antibiotic policy from time to time. This promotes rational antibiotic use and reduces resistance among bacteria.

Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates.

The lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates. In this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p<0.05. Among the total isolates (n = 226), TCPM detected 140 (61.95%) biofilm producers, with CoNS (9/9) (p<0.001) as the predominant biofilm former. When compared to TCPM, TM (n = 119) (p<0.001) showed 90.8% sensitivity and 70.1% specificity, CRA (n = 88) (p = 0.123) showed 68.2% sensitivity and 42% specificity, and MCRA (n = 86) (p = 0.442) showed 65.1% sensitivity and 40% specificity. Juxtaposed to CRA, colonies formed on MCRA developed more intense black pigmentation from 24 to 96 hours. There were 77 multi-drug-resistant (MDR)-biofilm formers and 39 extensively drug-resistant (XDR)-biofilm formers, with 100% resistance to ampicillin and ceftazidime, respectively. It is suggested that TM be used for biofilm detection, after TCPM. Unlike MCRA, black pigmentation in colonies formed on CRA declined with time. MDR- and XDR-biofilm formers were frequent among the clinical isolates.

Antibiotic resistance pattern of aerobic bacterial isolates from patients with skin and soft tissue infections in Karaikal

Skin and Soft Tissue Infections (SSTIs) are considered as non-fatal burden with significant morbidity and disability. The important challenge of severe SSTIs is choosing a drug for empirical treatment. From this region, only limited local antibiogram data is available. To determine the frequency of different aerobic bacteria isolated from patients with SSTIs attending dermatology and surgery departments of GH, Karaikal and to study the antibiotic resistance pattern of the isolates. This is a prospective, cross-sectional study with 100 samples. Standard protocol was followed for collection, processing, identification and antibiotic susceptibility testing. All isolates of and were screened for methicillin resistance and subsequently subjected to Oxacillin E-strip and Vancomycin E-strip to know the minimum inhibitory concentration (MIC) value. Isolates of Gram negative bacilli resistant to one or more carbapenems were tested for carbapenemase production using Modified Carbapenem Inactivation Method (MCIM) and multi drug resistant (MDR) organisms were identified. Most effective antibiotic for methicillin sensitive (MSSA) are Clindamycin (82.75%), Gentamicin (80.95%) and Cotrimoxazole (75%). The methicillin resistant (MRSA) incidence is 6.89% (2/29). Around 66.67% (4/6) of was Cefoxitin resistant. The carbapenem resistance was found to be 13.88% (5/36). Around 43.13% (22/51) Gram negative bacilli were MDR. The presence of MRSA and carbapenemase producing Gram negative bacilli are worrisome. Further, routine surveillance is needed to monitor the trends in antibiotic resistant pattern. However, this data paves way for judicious use of antibiotics for treatment and to prevent development of resistance in future.

Vulvovaginitis due to methicillin-resistant and methicillin-sensitive Staphylococcus aureus among women in reproductive age-group

Abstract Background: Although Staphylococcus aureus is commensal in 20% of individuals, its pathogenic consequences have not been unidentified. Colonisation of methicillin-resistant S. aureus (MRSA) in the vagina is associated with recurrent vaginal infections, skin and soft tissue infections surrounding the genital and buttock area and infertility. Methods: This study aimed at identifying S. aureus in vaginitis and the antibiotic susceptibility of S. aureus. Results: Of 463 patients’ vaginal swabs, 53 had significant growth, yielding 60 aerobic bacterial isolates. Seven (15%) isolates were S. aureus, 78% were MRSA and 100% were resistant to penicillin. All were sensitive to doxycycline and linezolid. Because the vaginal cavity is a reservoir for S. aureus, genital infections are considered, especially if they are recurring. Conclusions: Among patiens with vaginitis, MRSA is a common aetiological cause and should be tested for.

Monitoring trends in antimicrobial susceptibility patterns of bacterial isolates from horses with ulcerative keratitis

SummaryBackgroundUlcerative bacterial keratitis can result in loss of vision or the eye. Antimicrobial susceptibility data are essential in selecting optimal therapy while pending culture results.ObjectivesTo document aerobic bacterial isolates and antimicrobial susceptibilities from horses with ulcerative keratitis and report susceptibility patterns compared to previously described data from the same hospital.Study designRetrospective clinical case series.MethodsMedical records from horses with positive bacterial cultures and a clinical diagnosis of infectious ulcerative keratitis at the University of Tennessee Veterinary Medical Center between March 2011 and December 2020 were reviewed and compared to previously published data from January 1993 to May 2004.ResultsThirty‐one bacterial isolates were cultured from 27 samples (26 horses). The most common bacterial genera were Streptococcus (35%), Staphylococcus (29%) and Pseudomonas (13%), with a shift towards more Staphylococcus and less Streptococcus compared to previous years. There were minimal temporal changes in susceptibilities for these three genera. Staphylococcus spp. were statistically more likely to be cultured than other bacteria after the use of topical neomycin‐polymyxinB‐dexamethasone (p = 0.008). In vitro antimicrobial susceptibility results suggest that targeted combination therapy covered a greater percentage of isolates, compared with monotherapy, but in vitro results may not mirror in vivo results.Main limitationsInconsistencies in available medical data, selection bias and a small sample size are inherent limitations in this retrospective study, along with the inability to test against all available ophthalmic medications, challenges of interpreting cultures with limited veterinary susceptibility data and no susceptibility guidelines for topical medications.ConclusionLimited changes have occurred over the past several decades in corneal bacterial isolates and susceptibilities within this equine ophthalmology referral population, with a shift in more Staphylococcus cultured over time. Monitoring temporal trends of bacterial isolates and antimicrobial susceptibility data is important in every hospital.

Biodegradation of Chlorpyrifos by Soil-derived Aerobic Consortia and Bacterial Isolates

Biodegradation of Chlorpyrifos by Soil-derived Aerobic Consortia and Bacterial Isolates

Culture and sensitivity pattern of aerobic bacterial isolates in diabetic foot infections during 2018-2022 in Asian countries: a literature review study.

This research aims to determine the culture and sensitivity trend of aerobic bacterial isolates of DFI in Asian nations over a 5-year period. The article was searched using PubMed and Google Scholar with the keywords 'Diabetic Foot Infections', 'Antibiotic', 'Microbiological Profile', and their combinations. The author uses publications from 2018 to 2022 in Indonesian and English to select the appropriate journal. The author identified 11 relevant articles with microbiological profiles and sensitivity patterns in DFI. A total of 3097 isolates were found in 2498 patients with DFI. Gram-negative bacteria were the leading source of infection (n=1737; 56%). Totally, 1148 (or 37%) of all isolates were aerobic Gram-positive cocci. Staphylococcus aureus was the most commonly isolated aerobe (n=608, 20%), followed by Pseudomonas aeruginosa (n=451, 15%). Gram-positive bacteria showed good susceptibility to trimethoprim-sulfamethoxazole, chloramphenicol, doxycycline, vancomycin, and linezolid. Gram-negative bacteria displayed excellent susceptibility to aminoglycosides, piperacillin-tazobactam, and carbapenems. Gram-negative microorganisms were the most prevalent cause of DFI. This study's findings will facilitate the development of future empirical therapeutic guidelines for the treatment of DFI.

Comparative evaluation and prioritization of key influences on biodegradation of 2,2′,4,4′-tetrabrominated diphenyl ether by bacterial isolate B. xenovorans LB400

Polybrominated diphenyl ethers (PBDEs) are a class of persistent organic pollutants being widely distributed and harmful to human health and wildlife, and the development of sustainable rehabilitation strategies including microbial degradation is of great concern. Although the increasing number of bacteria, especially the broad-spectrum and potent aerobes have been isolated for the efficient removal of PBDEs, the external influences and the corresponding influential mechanism on biodegradation are not fully understood yet. Given the wide-spectrum biodegradability of aerobic bacterial isolate, B. xenovorans LB400 for PBDEs, the dual impacts of many pivotal factors including pH, temperature, presence of dissolved organic matter (DOM) and cadmium ion etc. were comprehensively revealed on biodegradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47). Due to the structural resemblance and stimulation of specific enzyme activity in bacteria, the biphenyl as substrates showed the greater capacity than non-aromatic compounds in improving biodegradation. The individual adaptation to neutrality and cultivation at about 30 °C was beneficial for biodegradation since the bacterial cellular viability and enzyme activity was mostly preserved. Although it was possibly good for the induction of hormesis and favorable to enhance the permeability or bioavailability of pollutant, the exceeding increase of Cd2+ or DOM may not give the profitable increase of biodegradation yet for the detrimental effect. For biodegradation, the mechanistic relationship that took account of the integrative correlation with the influential factors was artfully developed using partial least square (PLS) regression technique. Relative to the most significant influence of culture time and initial concentration of BDE-47, the larger relevance of other factors primarily marked as pH and DOM was consecutively shown after the quantitative prioritization. This may not only help understand the influential mechanism but provide a prioritizing regulation strategy for biodegradation of BDE-47. The PLS-derived relationship was validated with the certain predictability in biodegradation, and could be used as an alternative to accelerate a priori evaluation of suitability or improve the feasibility of such bacteria in remediation of PBDEs in the environment.

Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers.

The aim of the study was to identify the aerobic bacterial isolates and determine corresponding antibiotic susceptibility profiles in vitro in canine clinical specimens with stromal corneal ulcers, with the goal of providing recommendations for first-line treatment with antibiotics. A total of 198 canine corneal stromal ulcer samples were studied between 2018 and 2021. A corneal swab was collected and cultured under aerobic conditions. Bacterial organisms were identified at the species level by MALDI-TOF mass spectrometry. Antibiotic susceptibility testing for commonly used topical and systemic antibiotics was performed by disk diffusion. Bacterial growth was obtained from 80% of samples. A variety of bacterial species were identified wherein the most common specimens were represented by Staphylococcus pseudintermedius (22%), Staphylococcus epidermidis (12%), Staphylococcus capitis (11%), and Pseudomonas aeruginosa (10%). Based on the overall antibiotic susceptibility data, neopolybac alone (96%) or a combination of neopolybac with either ofloxacin or amikacin (each 99%) showed the best coverage for commonly isolated bacterial organisms from canine corneal stromal ulcers. Results of this study support the use of the combined antibiotics as the first-line response for the treatment of canine corneal stromal ulcers. A statically significant increase in acquired bacterial resistance was detected during the longitudinal data observation.

Microbiological Profile of Diabetic Foot Ulcer Patients in a Tertiary Care Hospital in Kerala

Abstract Introduction: One of the major complications of diabetes mellitus is diabetic foot ulcer (DFU). Fifteen percent of diabetes mellitus patients develop DFUs and 84% of foot amputations. The present study was carried out to determine the aerobic bacterial isolates cultured from diabetic foot infections and their susceptibility to commonly used antibiotics. Aim: The aim of this study was to determine the aerobic bacterial isolates cultured from diabetic foot infections and their susceptibility to commonly used antibiotics. Materials and Methods: We conducted a prospective observational study involving 104 patients with DFUs, who attended the general surgery/endocrinology outpatient department of our institution over 18 months. Results: This study showed that the most common organisms present in diabetic foot infections were Gram-negative aerobes, which accounted for 73.4%, while Gram-positive was 26%. Pseudomonas aeruginosa (20.1%) was the most predominant single organism isolated from the ulcer, followed by Klebsiella pneumonia and Escherichia coli (15.6% each). Staphylococcus aureus (7.8%) was the most common Gram-positive infective bacteria in DFU. Pseudomonas showed high sensitivity towards cefepime (93.3%), amikacin (93.6%), meropenem (89.7%) and piperacillin-tazobactam (83.9%) and low resistance towards ceftazidime (6.9%). In the current study, K. pneumonia showed good sensitivity towards meropenem (81.8%), amikacin and imipenem (70% each). One of the most important observations was the high resistance rate towards ertapenem (63.6%) and cefepime (60%). E. coli showed 100% sensitivity towards amikacin; it also had good sensitivity towards meropenem (89.5%) and imipenem (87.5%). Proteus species showed 100% sensitivity towards piperacillin-tazobactam, amikacin and meropenem, while an alarming rate of resistance towards imipenem (71.4%). Enterococcus spp. was 100% sensitive to amikacin, vancomycin and linezolid. S. aureus was 100% sensitive to cloxacillin, cefuroxime and ceftriaxone, and also showed 66.7% sensitivity to clindamycin. We also found that all the patients with methicillin-resistant S. aureus in diabetic foot infection wound swabs were 100% sensitive to vancomycin, linezolid and teicoplanin. Conclusion: Appropriate management of DFU is of paramount importance as it can prevent limb amputation and even sometimes potentially life-threatening limb complications. Isolation of microbial agents and determination of the sensitivity/resistance of different antimicrobial drugs are vital for the in-hospital management of these patients. The development of multi-drug resistance can be prevented by the institution of an appropriate antibiotic regimen.

Isolation of Methicillin-Resistant Staphylococcus aureus from Wound Samples during the COVID-19 Pandemic: A Retrospective Study

Introduction: On March 11th, 2020, the World Health Organisation (WHO) declared the outbreak of the novel coronavirus disease caused by the Severe Acute Respiratory Syndrome Corona Virus 2 (SARSCoV-2) virus as a pandemic. This recently discovered β-coronavirus spread instantaneously across mainland China due to human-tohuman transmission and crossed international borders aided by intercontinental travel. In most nations, the logarithmic growth of the cases very quickly overwhelmed the healthcare system which led to the overcrowding of the hospitals and led to a sudden surge in Hospital-Acquired Infections (HAIs). Implementation of contact precautions was implemented to control cross-infection. Aim: To determine the effect of Coronavirus Disease-2019 (COVID-19) on the prevalence of HAIs with special emphasis on Staphylococcus aureus (S. aureus). Materials and Methods: This three-year retrospective study (September 2018-August 2021) was undertaken at an apex tertiary care institute in Northern India. Wound swabs were collected and processed in the laboratory for the presence of aerobic bacterial isolates. S. aureus was identified using conventional methods and antimicrobial sensitivity was performed by the Kirby-Bauer disc diffusion method. Data was entered in Microsoft Excel and later analysed in International Business Machines (IBM) Statistical Package for the Social Sciences (SPSS) version 22.0. Results: A steady increase in the isolation of MethicillinResistant Staphylococcus aureus (MRSA) was noted during the study period (60.5% in 2018 to 78.1% in 2021). A statistically significant increase was noted in the detection of MRSA after the onset of the COVID-19 pandemic (p=0.018) despite the reduced number of surgeries conducted in the institution and rigorous execution of contact precautions. Conclusion: There was an increase in the rate of MRSA isolation during the study period. The increase was significantly affected by the onset of COVID-19. To contain the spread of MRSA, novel methods including preoperative screening of patients undergoing elective surgeries and periodic screening of hospital staff need to be implemented along with standard infection control precautions at all times.

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Antibacterial resistant bacteria cause a big concern to poultry and to public health in general because some bacterial poultry pathogens can infect human or transfer their resistance ability to human pathogens. Non therapeutic use of antibacterial in poultry especially as growth promoters to increase feed conversion efficiency is thought to be one of the main causes of resistance. The study included pulmonary swab samples collected during necropsy from 120 poultry farms showing respiratory symptoms with mortality. The disc diffusion method for antibiotic sensitivity testing was performed and antibiotic disks for 21 antibiotics was used. The results showed that three antibacterials were sensitive to more than 50% of the isolates. The first is doxycycline and 69.9% of the isolates were sensitive. The second is Cefalexin with 60.5% sensitive isolates and third is Chloramphenicol with 55.2% sensitive isolates. In the rest of antibacterials, less than 50% were sensitive. Five isolates were found resistant to all antibacterials. Moreover, three samples were found negative with no bacterial growth. The present study concluded that 50% of the aerobic bacteria isolated from poultry lungs are resistant to 85% of the 21 antibiotics tested in the study.

Diagnostic test accuracy of an automated device for the MALDI target preparation for microbial identification

The objective of this study was to evaluate the performance of the Copan Colibrí™ against the manual preparation of the MALDI targets. We analyzed 416 (31 different species) non-duplicate strains covering the most important species identified in clinical routine. We also assessed the intra-strain repeatability between the comparable methods. We then analyzed the performance of this new method after implementation in routine on 12,253 aerobic bacterial isolates and yeasts, encompassing a total of 42 different species. Among the 416 strains analyzed, 6.3% (26/416) and 10.8% (45/416) had a score value < 2 when processed by the Colibri™ and manual method, respectively. Only 5.9% (9/152) of the Gram positive rods and cocci had a score values < 2 by the Colibri™ versus 20.4% (31/152) by the manual method. We confirmed that this relative superiority observed for the Colibri™ was due primarily in the use of the formic acid protocol. For the Gram-negative bacteria, the results of both methods were comparable; 6.6% (17/256) and 4.7% (12/256) had a score value < 2 by the Colibri™ and the manual method, respectively. After implementation in routine, the results according to the Biotyper score cut-off values were distributed as follows: < 1.70: 2.5% (304/12,253), 1.70–1.79: 1.9% (227/12,253), 1.80–1.89: 3.1% (377/12,253), 1.90–1.99: 6.7% (825/12,253), and ≥ 2: 85.9% (10,520/12,253). The Colibrí™ coupled to MALDI-TOF/MS revealed good performances and higher intra-strain repeatability as compared to the manual preparation of the MALDI targets.

Drug-Resistant Aerobic Bacterial Pathogens in Patients with Crocodile Bite Wounds Attending at Arba Minch General Hospital, Southern Ethiopia.

A polymicrobial infection containing a diverse range of drug-resistant bacteria worsens the success of treatment for crocodile bite wound infection. However, there is a dearth of data in Ethiopia, where crocodile bite injuries are relatively common, particularly in the area around Lake Abaya and Lake Chamo in southern Ethiopia. A hospital-based cross-sectional study was conducted from May 1st, 2021, to April 30th, 2022, at Arba Minch General Hospital. Eleven consenting patients with crocodile bite wounds were enrolled in this study. Wound swabs were collected aseptically following microbiological procedures. The diversity and type of aerobic bacterial pathogens, and drug-resistant patterns were used to determine and characterize the nature of crocodile bite wound infections. Among 11 patients with crocodile bite wounds, 72.7% (8/11) of wound samples were found to be culture-positive, with a total of 21 aerobic bacterial isolates. Gram-negative bacterial isolates were found in 57.1% (12/21). Triple-bacterial isolates were found in 62.5% (5/8) of wound samples, followed by 37.5% (3/8) of double bacterial isolates. Pseudomonas aeruginosa (n = 5), followed by Citrobacter spp. (n = 4), and coagulase-negative Staphylococci (CoNS) (n = 4) were frequently isolated bacteria. Methicillin resistance (MR-CoNS) was found in 75% of isolated CoNS. 6.7% of the isolated Enterococcus faecalis was vancomycin-resistant (VRE), while 60% of the isolated P. aeruginosa were piperacillin-resistant. Overall, 85.7% (18/21) of the isolates were found to be multidrug-resistant (MDR), with 55.6% (10/18) of them being Gram-negative MDR bacterial isolates. Numerous aerobic bacteria that are resistant to the majority of common antibiotics have been associated with infections in crocodile bite wounds. Thus, the key to providing empirical therapy for such wounds is detecting the drug-resistant pattern of bacterial isolates.

Bacteriological Profile of Diabetic Ulcer Foot and Their Antimicrobial Susceptibility Pattern in a Tertiary Care Hospital, Trichy

Background: Wound infections are the major factor for development of diabetic foot ulcer.Objectives: To identify the prevalence of aerobic bacterial isolates and its antimicrobial susceptibility pattern in diabetic foot ulcer patients.Methods: The Prospective observational study was undertaken from July 16th to September 16th 2017 in tertiary care teaching Hospital, Tiruchirappalli, Tamilnadu.Result: Seventy-three (73) organisms were isolated from 63 diabetic foot ulcer patients. Staphylococcus aureus (26%), E. coli (19.2%) and Pseudomonas aeruginosa (19.2%) were isolated. MRSA with 53% were showed 100% sensitivity to vancomycin, linizolid and teicoplanin. By phenotypic Confirmatory Disc Diffusion Test, 57.1% of E. coli were ESBL producers whereas 50% of Klebsiella species were ESBL producers. All ESBL producers showed 100% sensitivity to imipenem.Interpretation and Conclusion: The microbiological report helps for initiating the narrow spectrum antibiotic therapy and reduce the emergence of multidrug resistant organisms.

Common Microorganisms Present in Diabetic Foot Infection and It’s Spectrum of Antibiotic Sensitivity

Introduction: Diabetic patients have foot problems secondary to neuropathy, micro-vascular changes and impaired resistance to infection. They are at increased risk of infection and ulceration. Diabetic ulcers are frequently involved in both aerobe and anaerobe microorganisms. It is important to culture the specimen on several different media. The Gram stain can provide valuable information regarding the range of organisms under consideration. The aim of this study is to identify the sensitive antibiotics to the specific aerobic bacteria, to prevent the random misuse of broad spectrum antibiotics and make awareness about diabetic foot care. Materials and Methods: This study was a retrospective analysis and was carried out in general surgery and diabetic wards at North Bengal Medical College Hospital (NBMCH), Sirajganj, Bangladesh from August, 2019 to July, 2020. To identify common aerobic microorganism and spectrum of antibiotic sensitivity from 54 cases of diabetic foot ulcers, wound swabs taken and cultured using standard aerobic microbiological techniques. Antibiotic sensitivity testing to different antimicrobial agents were carried out using the disc diffusion method. Result: Among 54 aerobic bacterial isolates, Gram negative bacteria 41(75.9%) and Gram positive bacteria 13(24.1%). Escherichia Coli 28(51.9%), Proteus spp 09(16.7%), Pseudomonas 03(5.6%), Klebsiella spp 01(1.9%) were isolated Gram negative bacteria. Staphylococcus aureus 13(24.1%) was only Gram positive bacteria. Gram negative bacterial isolates were sensitive to Imipenem, while Vancomycin showed good activity against Gram positive bacteria. Conclusion: The antibiogram results of this study suggest that bacteria remain sensitive to a limited number of used agents, while found resistant to a number of widely used agents. Imipenem was most effective against Gram negative bacilli and also effective against Gram positive cocci. Medicine Today 2022 Vol.34(1): 65-69

Staphylococcus aureus-induced pyogranulomatous dermatitis, osteomyelitis, and meningitis with Splendore-Hoeppli reaction in a cat coinfected with the feline leukemia virus and Leishmania sp.

This report describes a case of a pyogranulomatous dermatitis, osteomyelitis, and meningitis with Splendore-Hoeppli reaction caused by Staphylococcus aureus in a sheltered female adult cat coinfected with the feline leukemia virus (FeLV) and Leishmania sp. The cat had a mild anemia and marked increased total leukocytes, particularly band and segmented neutrophils. The cat had laboratorial diagnosis of FeLV and Leishmania sp. infections. Clinically, the cat had extensive and multifocal areas of ulceration in cranial region. Due to the progression of cutaneous lesions, progressive weight loss, and the risk for other sheltered animals, the cat was euthanized. Microscopically, there was marked pyogranulomatous ulcerative dermatitis, osteomyelitis and meningitis, with multiple large intralesional colonies of Gram-positive cocci associated with Splendore-Hoeppli reaction. Aerobic bacterial isolates were identified as S. aureus by MALDI-ToF MS. Leishmania sp. DNA sequences were detected in liver and spleen, and amastigotes were demonstrated in skin sections by immunohistochemistry. In conclusion, here we describe a case of S. aureus-induced pyogranulomatous meningitis with SH reaction in a cat naturally coinfected with FeLV and Leishmania sp.