The ichnofossil Rhizocorallium—an oblique to horizontal U-shaped spreiten burrow—has been recognized in a broad range of Phanerozoic continental, marginal marine, and shallow to deep marine facies. Nonetheless, occurrences of this ichnotaxon in Cretaceous estuarine deposits are rarely described. Herein we document Rhizocorallium in the Ingersoll shale, a carbonaceous clay lens within an estuarine succession contained in the Upper Cretaceous (Santonian) Eutaw Formation exposed in the eastern U.S. Gulf coastal plain. The Ingersoll Rhizocorallium specimens, assigned to R. commune var. irregulare based on their basic morphology, horizontal disposition, dimensions, and actively filled spreiten, record a change in depositional conditions and substrate consistency linked to transgression. The Ingersoll shale accumulated rapidly in a restricted tidal channel in a bayhead delta setting and hosts an impoverished resident ichnofauna reflecting soupground conditions. In contrast, attributes of Rhizocorallium (e.g., burrow wall bioglyphs) and associated Thalassinoides in the upper part of the Ingersoll shale reflect firm substrates (i.e., Glossifungites ichnofacies). Firmground development and colonization reflect burial dewatering and subsequent exhumation by bay-shoreline ravinement of clays and the concomitant shift to estuarine central bay deposition. The biological affinity of the Rhizocorallium trace makers cannot be confidently established. However, based on relatively straight and seemingly current-aligned axes, passive burrow fills, lack of associated fecal pellets, and emplacement in firm clay, these structures were likely produced by suspension-feeding organisms—either crustaceans or worms—that took advantage of wave- or tide-induced currents in shallow areas near the estuary head.