Advanced Oxidation Processes For Treatment Research Articles

Predictive modeling of Enterococcus sp. removal with limited data from different advanced oxidation processes: A machine learning approach

The removal of contaminants through Advanced Oxidation Processes (AOPs) is a complex task that demands the simultaneous consideration of multiple operating parameters, such as type and concentration of oxidant and catalyst, type and intensity of radiation, composition of aqueous matrix, etc. Designing efficient AOPs often requires expensive and time-consuming laboratory experiments. To improve this process, this study proposes a Machine Learning approach based on a Random Forest (RF) model, to predict Enterococcus sp. concentration in wastewater treated with various AOPs, even when dealing with limited data. To assess our approach under diverse conditions, a data partitioning methodology is used to categorize the different AOPs into three distinct study cases of increasing complexity, from Case I to Case III. The evaluation of the RF model’s performance, combined with the data partitioning methodology, demonstrated its usefulness in predicting missing or additional disinfection values at any instant during the AOPs. Specifically, in Case I, the model excels at generalizing predictions across various AOP treatments, followed by Case II and III, which achieve Root Mean Squared Error (RMSE) values below or comparable to the average RMSE of Case I (0.72) in 8 out of 15 and 2 out of 4 treatments, respectively. Moreover, the effects of imbalanced data on model performance are discussed. This highlights the potential of our approach to assess AOPs performance and facilitate the design of new experiments of the same treatment type without the need for additional laboratory trials, even in challenging conditions.

Trace Co(II) triggers peracetic acid activation in phosphate buffer: New insights into the oxidative species responsible for ciprofloxacin removal

Peracetic acid (PAA) emerges as a promising disinfectant and oxidant applied worldwide, and its application has been broadened for advanced oxidation processes (AOPs) in wastewater treatment. Current studies on transition metal-activated AOPs utilized relatively high concentrations of catalysts, leading to potential secondary pollution concerns. This study boosts the understanding of reaction mechanism in PAA activation system under a low-level concentration. Herein, trace levels of Co(II) (1 μM) and practical dosages of PAA (50–250 μM) were employed, achieving noticeable ciprofloxacin (CIP) degradation efficiencies (75.8–99.0%) within 20 min. Two orders of magnitude of the CIP’s antibacterial activity significantly decreased after Co(II)/PAA AOP treatment, which suggested the effective ecological risk control capability of the reaction system. The degradation performed well in various water matrices and the primary reactive species is proposed to be CoHPO4-OO(O)CCH3 complexes with scavenging tests and electron paramagnetic resonance tests. The degradation pathway of fluoroquinolones including piperazine ring-opening (dealkylation and oxidation), defluorination, and decarboxylation, were systematically elucidated. This study boosts a comprehensive and novel understanding of PAA-based AOP for CIP degradation.

Comparison of photoinduced and electrochemically induced degradation of venlafaxine.

The European Union requires environmental monitoring of the antidepressant drug venlafaxine. Advanced oxidation processes provide a remedy against the spread of micropollutants. In this study, the photoinduced and electrochemical decompositions of venlafaxine were investigated in terms of mechanism and efficacy using high-performance liquid chromatography coupled to high-resolution multifragmentation mass spectrometry. Kinetic analysis, structure elucidation, matrix variation, and radical scavenging indicated the dominance of a hydroxyl-mediated indirect mechanism during photodegradation and hydroxyl and direct electrochemical oxidation for electrochemical degradation. Oxidants, sulfate, and chloride ions acted as accelerants, which reduced venlafaxine half-lives from 62 to 25 min. Humic acid decelerated degradation during ultra-violet irradiation up to 50%, but accelerated during electrochemical oxidation up to 56%. In silico quantitative structure activity relationship analysis predicted decreased environmental hazard after advanced oxidation process treatment. In general, photoirradiation proved more efficient due to faster decomposition and slightly less toxic transformation products. Yet, matrix effects would have to be carefully evaluated when potential applications as a fourth purification stage were to be considered.

Advanced oxidation processes may transform unknown PFAS in groundwater into known products

Per- and polyfluoroalkyl substances (PFAS) are a group of fluorinated organic contaminants classified as persistent in the aquatic environment. Early studies using targeted analysis approaches to evaluate the degradation of PFAS by advanced oxidation processes (AOP) in real water matrices may have been misinterpreted due to the presence of undetected or unknown PFAS in these matrices. The aims of the present study were to (1) screen selected commercially available AOPs (UV, UV + H2O2, O3/H2O2) and UV photocatalysis in a pilot system using commercially used and novel photocatalysts (TiO2, boron nitride [BN]) for removing PFAS contaminants and (2) evaluate their role on the conversion of non-detected/unknown to known PFAS compounds in real groundwater used as drinking water supplies. Results indicated that, while AOPs have the potential to achieve removal of the EPA method 533 target PFAS compounds (PFDA [100%], PFNA [100%], PFOA [85–94%], PFOS [25–100%], PFHxS [3–100%], PFPeS [100%], PFBS [100%]), AOPs transformed non-detected/unknown longer-chain PFAS compounds to detectable shorter-chain ones under very high-dose AOP operating conditions, leading to an increase in ∑PFAS concentration ranging from 95% to 340%. As emerging PFAS treatment processes transition from lab-scale investigations of target PFAS to pilot testing of real water matrices, studies will need to consider impact of the presence of non-target long-chain PFAS to transform into targeted PFAS compounds. A promising approach to address the potential risks and unforeseen consequences could involve an increased reliance on adsorbable organic fluorine (AOF) analysis before and after advanced oxidation process (AOP) treatment.

Examining the immunotoxicity of oil sands process affected waters using a human macrophage cell line

Oil sands process affected water (OSPW) is produced during the surface mining of the oil sands bitumen deposits in Northern Alberta. OSPW contains variable quantities of organic and inorganic components causing toxic effects on living organisms. Advanced Oxidation Processes (AOPs) are widely used to degrade toxic organic components from OSPW including naphthenic acids (NAs). However, there is no established biological procedure to assess the effectiveness of the remediation processes. Our previous study showed that human macrophage cells (THP-1) can be used as a bioindicator system to evaluate the effectiveness of OSPW treatments through examining the proinflammatory gene transcription levels. In the present study, we investigated the immunotoxicological changes in THP-1 cells following exposure to untreated and AOP-treated OSPW. Specifically, using proinflammatory cytokine protein secretion assays we showed that AOP treatment significantly abrogates the ability of OSPW to induce the secretion of IL-1β, IL-6, IL-8, TNF-α, IL-1Ra and MCP-1. By measuring transcriptional activity as well as surface protein expression levels, we also showed that two select immune cell surface markers, CD40 and CD54, were significantly elevated following OSPW exposure. However, AOP treatments abolished the immunostimulatory properties of OSPW to enhance the surface expression of these immune proteins. Finally, a transcriptome-based approach was used to examine the proinflammatory effects of OSPW as well as the abrogation of immunotoxicity following AOP treatments. Overall, this research shows how a human macrophage cell-based biomonitoring system serves as an effective in vitro tool to study the immunotoxicity of OSPW samples before and after targeted remediation strategies.

Integrated Fenton-like and ozonation based advanced oxidation processes for treatment of real-time textile effluent containing azo reactive dyes

The treatment of real-time textile effluent, collected from the Common Effluent Treatment Plant (CETP) of Kerala Industrial Infrastructure Development Corporation (KINFRA) at Kannur (District), Kerala (State), India, have been studied by utilizing the Fenton-like and ozone (O3) based advanced oxidation processes (AOPs). The Fenton-like AOP has been conducted as the pre-treatment of textile effluent involving the activation of persulfate (PS) and hydrogen peroxide (H2O2) as a single and the mixed oxidants by using the Flyash (FA)-Pd composite particles as the activator. The maximum chemical oxygen demand (COD) removal of 84% has been observed for a stand-alone O3 based treatment at an O3 flow rate of 5–6 g h−1. By conducting the pre-treatment of textile effluent with the PS, H2O2, and mixed oxidants (PS and H2O2) based Fenton-like AOPs, the COD removal after an O3 based post-treatment has been observed to be 83, 87, and 93% respectively at an O3 flow rate of 2, 3, and 5 g h−1. Hence, the Fenton-like pre-treatment involving the activation of mixed oxidants has been determined to be the best method for the highest COD removal of real-time textile effluent. The optimum values of initial oxidant-ratio (initial [H2O2]:initial [PS]), initial oxidant-dosage, and ozonation time, for the mixed oxidants based Fenton-like pre-treatment, have been determined to be 3 wt% mM−1, 6:2 wt% mM−1, and 60 min respectively. Under the most optimum conditions, the COD removal has been attributed to the combination of O2•- (for the pre-treatment) and •OOH (for the post-treatment) which possess relatively lower oxidation potential values.

Removal of typical sulfonamide resistance genes by ultraviolet/hydrogen peroxide advanced oxidation process in mariculture wastewater

Antibiotics are extensively used in the mariculture sector, leading to the emergence and dissemination of antibiotic resistance genes (ARGs) and posing a serious threat to public health. Therefore, it is urgent to remove and control of ARGs in the mariculture environment. In this study, in order to assess the efficiency and mechanism of ARGs removal, we employed the ultraviolet/hydrogen peroxide (UV/H2O2) advanced oxidation process (AOP) to treat mariculture wastewater containing prevalent sulfonamide resistance genes, namely sul1, sul2, and intl1. The results demonstrated that UV/H2O2 AOP effectively eliminated ARGs, achieving a significant reduction of 2.51–2.71 log for 16S rRNA, intl1, sul1, and sul2 genes after 10 min of treatment with a UV dose of 570 mJ·cm−2, 0.01 mol·L−1 H2O2, and pH 3. The removal of ARGs was influenced by the concentration of the oxidizing agent. Specifically, as the H2O2 concentration increased, the removal of ARGs initially increased and then decreased. Moreover, UV/H2O2 AOP exhibited different removal efficiencies for intracellular ARGs (iARGs) and extracellular ARGs (eARGs). The absolute abundance of iARGs gradually decreased, the eARGs were less impacted by the UV/H2O2 AOP treatment. Furthermore, the microbial community exhibited distinct sensitivities to UV/H2O2 AOP and UV treatment. Proteobacteria and Campylobacterota consistently appeared as dominant phyla, with Sulfurovum being the dominant genus. These findings shed light on the control of ARGs in mariculture wastewater and confirmed the promising prospects of UV/H2O2 AOP as an effective approach.

Research Progress of Industrial Heavy Metal Wastewater Treatment Process

With the rapid development of modern industry, the types and emissions of heavy metal wastewater are also increasing day by day. Heavy metal wastewater is non biodegradable, and discharge standards are also updated and iterated with people's continuous requirements for health. Based on the current situation of industrial heavy metal wastewater treatment in China, this paper summarizes the sources and treatment status of heavy metal wastewater, as well as the commonly used technologies and processes for industrial wastewater treatment from traditional to modern at home and abroad, introduces the advantages and disadvantages of physical, chemical and biological treatment methods for industrial wastewater containing heavy metals in practical applications, and looks forward to the development trend of Advanced oxidation process and biological treatment methods. The biological treatment method has the characteristics of rich raw materials, environmentally friendly and pollution-free, but at present, the research on biotechnology is not mature and its application is not widespread. If the system is applied to industrial treatment of heavy metal wastewater, further research is still needed. There are few heavy metal wastewater treatment equipment or systems developed from heavy metal wastewater treatment processes, and the issues of continuous operation, automation, and large-scale treatment of heavy metal wastewater still need to be explored. There is also room for development in the field of resource development and utilization by extracting and recovering heavy metals from sludge after heavy metal wastewater treatment. This article reviews the methods for treating heavy metal wastewater and evaluates them, with the aim of pointing out research directions and providing practical application references for the large-scale industrial development of industrial heavy metal wastewater in the future.

Effect of the Catalyst Support on Dye Degradation Using Advanced Oxidation Processes Treatment: Application of the Fractional Factorial Design

Effect of the Catalyst Support on Dye Degradation Using Advanced Oxidation Processes Treatment: Application of the Fractional Factorial Design

Photolysis of chloramines in vacuum-UV and vacuum-UV/chlorine advanced oxidation processes for removal of 1,4-dioxane: Effect of water matrix, kinetic modeling, and implications for potable reuse

Advanced oxidation processes (AOPs) are a key step in eliminating persistent micropollutants in potable reuse trains. Under such conditions, chloramines are an inevitable component in the AOP feed water given their application as an antifouling agent for the upstream membrane processes. In cases when other oxidants, such as free chlorine, are to be used in the AOP treatment, the effect of background chloramines and any potential interplays between the oxidants should be considered. In this study, vacuum-UV (VUV) and VUV/Cl2 have been proposed as promising AOP alternatives for potable reuse and the effect of chloramine photolysis has been considered on the removal of 1,4-dioxane. Results indicated that while presence of chloramine reduces the treatment efficiency in the VUV AOP, coexistence of free chlorine and chloramine oxidants significantly improves 1,4-dioxane degradation rates. Experimental data and kinetic modeling both confirmed the roles of OH• and Cl2•- in 1,4-dioxane removal with 62.5% and 32.5% contribution in the VUV/Cl2/chloramines, respectively. Among the other water matrix conditions, Cl- was shown to improve the degradation rates while HCO3- suppressed the reactions by scavenging radical species. Overall, the findings of this research are informative for the design and development of VUV AOPs at small scale potable reuse facilities.

The Material Matters: Sorption/Desorption Study of Selected Estrogens on Common Tubing or Sampling Materials Used in Water Sampling, Handling, Analysis or Treatment Technologies

Plastic/rubber materials used as fasteners in equipment for analyzing or removing organic pollutants in water treatment technologies form an essential part of the device. Micropollutants in water are typically present at very low concentrations (ng/L to µg/L). Therefore, when designing, for example, units for advanced oxidation processes (AOPs) or planning sample handling, it is necessary to assess whether the material is compatible with the usually hydrophobic nature of the pollutants. As a model example, the possible interactions of estrogens, namely, estrone (E1), 17β-estradiol (E2), estriol (E3) and 17α-ethinylestradiol (EE2) with six commonly used plastic and rubber materials were investigated at environmentally relevant concentrations (100–500 ng/L). In the first phase, we proved that polyvinyl chloride (PVC), polytetrafluoroethylene (PTFE), polyvinylidene fluoride (PVDF) and ethylene propylene diene monomer (EPDM) materials adsorbed only negligible amounts of estrogens, while significant amounts of E1, E2 and EE2 were adsorbed onto Tygon S3™ material. Another unsuitable material was styrene butadiene rubber (SBR), sorbing a considerable quantity of estrone. A detailed test of EPDM at higher concentrations (300 and 500 ng/L) and prolonged soaking time showed significant sorption of EE2 after 12 h of soaking in both deionized and tap water matrices. Thus, EPDM, PTFE and PVDF are suitable materials for sample handling or producing devices for AOP treatment due to their chemical inertness and mechanical flexibility. The results suggest that plastic materials that come into contact with contaminated water must be carefully selected, especially when working at environmentally relevant concentrations.

A review of advanced oxidation process towards organic pollutants and its potential application in fracturing flowback fluid.

Fracturing flowback fluid (FFF) including various kinds of organic pollutants that do harms to people and new treatments are urgently needed. Advanced oxidation processes (AOPs) are suitable methods in consideration with molecular weight, removal cost and efficiency. Here, we summarize the recent studies about AOP treatments towards organic pollutants and discuss the application prospects in treatment of FFF. Immobilization and loading methods of catalysts, evaluation method of degradation of FFF, and continuous treatment process flow are discussed in this review. In conclusion, further studies are urgently needed in aspects of catalyst loading methods, macromolecule organic evaluation methods, industrial process, and pathways of macromolecule organics' decomposition.

Effects of Advanced Oxidation on Wastewater Effluent Ecotoxicity: A Novel Assessment through the Life History and Lipidomics Analysis of Daphnia magna

Advanced oxidation processes (AOPs) generate reactive radicals to effectively degrade pharmaceuticals and personal care products (PPCPs) in wastewater that are not removed during conventional biological treatment. However, transformation products from AOPs may still be biologically active and have deleterious effects on aquatic organisms. This study is based on an integrated approach using targeted PPCP analysis, Daphnia survivability and life history, and high-resolution mass spectrometry-based targeted lipidomics analysis to assess the performance of a novel AOP ultraviolet/peracetic acid (UV/PAA) and conventional UV/hydrogen peroxide (UV/H2O2). Both AOP treatments significantly decreased PPCP concentrations compared to the secondary activated sludge treatment alone. Daphnia survivability tests showed no significant differences between the control group and the treated group exposed over 5 days to AOP-treated wastewater. In contrast, a 14 day life history assessment and associated lipidomics showed that while 6 mg/L UV/H2O2 or UV/PAA treatment decreased wastewater toxicity to an overall level that is comparable with the control, the 3 mg/L UV/PAA AOP treatment did not achieve the same decrease in toxicity. At the latter condition, Daphnia exhibited smaller growth, significant changes in lipids, and lower rate of population increase than the control. The use of integrated methods allowed for the elucidation of sub-lethal effects of AOP-treated wastewater on Daphnia.

Coupled cavitation and AOPs treatment of Primene-JMT containing wastewater

The application of various cavitational reactors as the novel approach to remove the emerging contaminant, Primene-JMT (PJMT), in an aqueous solution has been elucidated in this work. Ultrasonic step horn (US step horn), longitudinal horn based ultrasound (LHUS), hydrodynamic cavitation (HC) with single slit venturi (HCSV), and dual slit venturi (HCDV) are the specific reactors studied individually and in combination with Advanced Oxidation Processes (AOPs) involving H2O2, Fenton, O3, and NaClO. Different operating parameters such as oxidant loadings, oxidant ratios, treatment time, and the inlet pressure have been varied to elucidate the optimum conditions. The optimal conditions established in the study are H2O2 dosing of 0.8 g/L, H2O2/Fe2+ ratio of 4/1, O3 flow rate of 3LPH, and NaClO dosing of 0.8 g/L as applicable for the specific process. The US+NaClO and US+Fenton+CaO schemes were shown to be most effective at laboratory scale in achieving a maximum COD reduction of 98 % each at pH of 9 after 120 min and 130 min, respectively. Under the optimum conditions, LHUS+NaClO and HCDV+NaClO schemes were also successfully applied on pilot scale, showing the maximum COD reductions of 90 % and 96 % and cavitational yield of 0.89×10−3mg/J and 26.34×10−3mg/J, respectively. Comparison of different cavitating devices revealed that a dual slit venturi was nearly 30 % more efficient than a single slit venturi under similar conditions. It was clearly demonstrated that a combination strategy under optimum conditions was advantageous for attaining the highest COD reduction and cavitational yield at the lower operational cost.

Activation of persulfate using copper oxide nanoparticles for the degradation of Rhodamine B containing effluents: Degradation efficiency and ecotoxicological studies

The development and implementation of advanced oxidation processes (AOP) are intended to assist in the treatment of effluents produced during anthropogenic activities, reducing the release of chemicals to the environment and consequently their potential toxicity to the environment. The present work is intended to assess the chemical and ecotoxicological efficiency of an AOP that uses copper oxide nanoparticles (CuO-NP) and persulfate (PS, as an oxidation agent) for the removal of Rhodamine B (RhB) from an aqueous solution. For this, CuO-NPs were prepared and used as a sole catalyst or combined with thermal treatment to activate PS for the degradation of RhB (in distilled water, DI, and in ASTM medium). Kinetic and mechanistic studies were employed to understand the performance of the system. The lethal ecotoxicity of the untreated and treated RhB solution was characterized using rotifer species Brachionus calyciflorus. Complete removal of RhB was achieved at [RhB]0 = 50 mg/L, [PS]0 = 1 mM, CuO = 0.5 g/L under pH = 7.5 at 45 °C for DI and ASTM. Brachionus calyciflorus mortality pointed to a very high ecotoxicity of the effluent before and after the AOP treatment; the former was less toxic than the latter: LC50,24h = 44.3% and 8.24%, respectively. Mortality observed in the treated effluent was most probably due to the presence of reaction products (e.g., activated PS) rather than RhB, since CuO/PS system was able to remove >90% of RhB. The obtained results demonstrated that although the proposed method is applicable to deal with high concentrations of recalcitrant organic compounds, the treated effluents can cause high ecotoxicity when released into the environment.

A detailed review on advanced oxidation process in treatment of wastewater: Mechanism, challenges and future outlook

The presence of several contaminants in waterbodies raises global pollution and creates major risks to mankind, wildlife, as well as other living organisms. Development of an effective, feasible, cost-effective and eco-friendly approach for treating wastewater that is discharged from various industries is important for bringing down the deposition of contaminants into environment. Advanced oxidation process is an efficient technique for treating wastewater owing to its advantages such as high oxidation efficacy and does not produce any secondary pollutants. Advanced oxidation process can be performed through various methods such as ozone, Fenton, electrochemical, photolysis, sonolysis, etc. These methods have been widely utilized for degradation of emerging pollutants that cannot be destroyed using conventional approaches. This review focuses on wastewater treatment using advanced oxidation process. A brief discussion on mechanism involved is provided. In addition, various types of advanced oxidation process and their mechanism are explained in detail. Challenges faced during wastewater treatment process using oxidation, electrochemical, Fenton, photocatalysis and sonolysis are discussed elaborately. Advanced oxidation process can be viewed as potential approach for treating wastewater with certain modifications and solving challenges.

Treatment of wood by sulfate and hydroxyl radical oxidation produced from thermally-activated persulfate: VOC emission and wood property evaluation

ABSTRACT Wood emits volatile organic compounds (VOCs) that can affect people’s health and air quality. Based on advanced oxidation processes (AOPs), sulfate radicals produced via thermally-activated persulfate (Na2S2O8, KHSO5) can minimize VOCs. This research aims to apply this technique to minimize the VOC content in Pinus radiata. The results showed that terpenes and alcohols were the main VOCs emitted from Pinus radiata, which were greatly minimized using four treatments; however, acetic acid and 3-furaldehyde emissions increased. Furthermore, the temperature and oxidizing agent contributed to VOC degradation. Characterization results, including FTIR, XRD, color tests, and compressive strength indicated that the AOP treatment caused only a slight decline in the wood properties, while effectively reducing VOC emissions by as much as 32.75–61.05%.

Production of medium-chain carboxylic acids using sewage sludge pretreated by combined Fenton and persulfate oxidation

Medium chain carboxylates (MCCs) production from sewage sludge (SS) is an economically and environmentally feasible technology for recovering value-added chemicals from SS. To improve MCCs production from SS, Fe (II)-mediated advanced oxidation processes (AOPs) including Fenton oxidation (FO), persulfate oxidation (PO), and combined Fenton-persulfate oxidation (CFP) were adopted to examine their effect on fermentation. Highest total MCCs concentration of 3445.85 mg COD/L was achieved in CFP treated group, followed by PO treated group (2678.78 mg COD/L), control group (2367.72 mg COD/L) and FO treated group (2294.14 mg COD/L). MCCs produced in all groups were dominated by caproate, and all three AOPs treatment promoted longer-chain MCCs (i.e. heptylate and caprylate) production. Microbial analysis showed that genus Romboutsia, Paraclostridium, Clostridium_sensu_stricto_1, norank_f_Anaerolineaceae and Acetobacterium that were positively correlated with MCCs production were significantly enriched in CFP treated group, which contributed to the highest MCCs production in CFP treated group. The functional enzymes analysis showed that both PO and CFO treatment promoted MCCs production by strengthening the fatty acid biosynthesis pathway during the chain elongation (CE) process.

Comparison of homogeneous and heterogeneous electrochemical advanced oxidation processes for treatment of textile industry wastewater

This study aimed at understanding the influence of the generation of oxidants in a heterogeneous way at boron-doped diamond (BDD) anode (anodic oxidation (AO)) or homogeneously in the bulk (electro-Fenton (EF)) during treatment of a textile industry wastewater. Both processes achieved high TOC removal. A yield of 95 % was obtained by combining EF with BDD anode during 6 h of treatment. The EF process was found to be faster and more efficient for discoloration of the effluent, whereas AO was more effective to limit the formation of degradation by-products in the bulk. An advantage of AO was to treat this alkaline effluent without any pH adjustment. Operating these processes under current limitation allowed optimizing energy consumption in both cases. However, using BDD anode led to the formation of very high concentration of ClO3-/ClO4- from Cl- oxidation (even at low current density), which appears as a key challenge for treatment of such effluent by AO. By comparison, EF with Pt anode strongly reduced the formation of ClO3-/ClO4-. Operating EF at low current density even maintained these concentrations below 0.5 % of the initial Cl- concentration. A trade-off should be considered between TOC removal and formation of toxic chlorinated by-products.

Chelation and AOP Approach on Mn-Metal and COD Reduction of Liquid Laboratory Waste

Heavy metal waste and organic material used in flushing laboratory glassware can accumulate and potentially damage the environment. Thus, it needs to be processed before being disposed of to avoid any damage to the aquatic ecosystem. This research aimed to determine the pH wastewater sample and APDC concentration as chelating agents that would be the best for chelation treatment and determine the H2O2 concentration and UV exposure duration to identify the best treatment for the advanced oxidation process. This research was expected to find an alternative in wastewater treatment and provide an overview of the interaction of chelation treatment with organic pollutants as the basis for AOP-treatment management. Laboratory wastewater quality has improved through the extraction treatment of chelate from the pH 4 experiment, ammonium pyrrolidine dithiocarbamate (APDC) concentration at 7.5% with the manganese metal value decreased by 53%, an advanced oxidation process (AOP) treatment of H2O2 concentration at a 5.10-5%, and 31-hour UV exposure, resulting in chemical oxygen demand (COD) decreased by 98.98%. Waste containing both materials was reduced by combining the extraction of chelate formation and AOP, which was detected from the presence of manganese metal and the COD. This treatment can be an appropriate and easy way to degrade laboratory waste in a mini wastewater treatment process. This research succeeded in finding several novelties: first, a suitable pH value to reactivate Mn metal for chelation with APDC; second, the concentration of APDC determined to optimize the Mn metal chelate; third, the concentration of H2O2 to increase the effectiveness of organic matter reduction; fourth, the duration of UV irradiation is effective and efficient as a photocatalyst to reduce organic matter.