Advanced Developmental Stages Research Articles

Conservation Management Practices for Biodiversity Preservation in Urban Informal Green Spaces: Lessons from Central European City

Urban informal green spaces (IGS) represent valuable reservoirs of biodiversity within urban areas and are increasingly recognized as integral components of green infrastructure. They are perceived as temporary ecosystems, and the management of their vegetation is relatively understudied. The development time of spontaneous vegetation on transformed lands is considered to be in the range of decades, which makes it even more necessary to provide managers with better guidelines for such a long period. Two suggested management approaches for these areas involve: (1) retaining vegetation at various stages of succession (non-forest IGS) and (2) protecting advanced developmental stages (forest IGS), with options for balanced intervention or complete non-intervention. However, the differences in biodiversity between these two types in cities across Central Europe remain unknown, as well as whether the predictors of biodiversity at both local and landscape scales are consistent for non-forest and forest IGS. We examined factors such as habitat continuity, landscape structure, soil quality, and human impact to shed light on pathways for enhancing urban floristic diversity. Conducting extensive botanical surveys in existing informal green spaces (IGS) in Warsaw, we derived various parameters, including the total number of species, Shannon-Wiener biodiversity index, hemeroby, urbanity, share of species from distinct ecological groups, and the number of rare and ancient forest plant species. Tracing habitat continuity from the early 20th century using digitized aerial imagery provided a unique long-term perspective on IGS development. We revealed that no management is pivotal for the conservation of select rare and ancient forest species. On the other hand, partial abandonment with occasional maintenance may enrich species diversity across different successional phases. We uncovered the significant influence of landscape structure and human activity on vegetation species composition within IGS. Notably, IGS proximate to extensive forest landscapes displayed a marked abundance of forest species, alongside a greater prevalence of rare species. However, the presence of other vegetation types in the vicinity did not yield similar effects. Our findings indicate that IGS, when left untouched for decades near forested areas, are valuable for urban biodiversity. As cities across the globe seek sustainable paths, this research underscores the importance of properly understanding and integrating IGS into urban ecological planning.

Long-term detection of Hartmannibacter diazotrophicus on winter wheat and spring barley roots under field conditions revealed positive correlations on yield parameters with the bacterium abundance.

Monitoring of bioinoculants once released into the field remains largely unexplored; thus, more information is required about their survival and interactions after root colonization. Therefore, specific primers were used to perform a long-term tracking to elucidate the effect of Hartmannibacter diazotrophicus on wheat and barley production at two experimental organic agriculture field stations. Three factors were evaluated: organic fertilizer application (with and without), row spacing (15 and 50 cm), and bacterial inoculation (H. diazotrophicus and control without bacteria). Hartmannibacter diazotrophicus was detected by quantitative polymerase chain reaction on the roots (up to 5× 105 copies g-1 dry weight) until advanced developmental stages under field conditions during two seasons, and mostly in one farm. Correlation analysis showed a significant effect of H. diazotrophicus copy numbers on the yield parameters straw yield (increase of 453 kg ha-1 in wheat compared to the mean) and crude grain protein concentration (increase of 0.30% in wheat and 0.80% in barley compared to the mean). Our findings showed an apparently constant presence of H. diazotrophicus on both wheat and barley roots until 273 and 119 days after seeding, respectively, and its addition and concentration in the roots are associated with higher yields in one crop.

Engineered polyspecific antibodies: A new frontier in the field of immunotherapeutics.

The 21st-century beginning remarked with the huge success of monospecific MAbs, however, in the last couple of years, polyspecific MAbs (PsAbs) have been an interesting topic and show promise of being biobetter than monospecific MAbs. Polyspecificity, in which a single antibody serves multiple specific target binding, has been hypothesized to contribute to the development of a highly effective antibody repertoire for immune defence. This polyspecific MAb trend represents an explosion that is gripping the whole pharmaceutical industry. This review is concerned with the current development and quality enforcement of PsAbs. All provided literature on monospecific MAbs and polyspecific MAbs (PsAbs) were searched using various electronic databases such as PubMed, Google Scholar, Web of Science, Elsevier, Springer, ACS, Google Patent and books via the keywords Antibody engineering, Polyspecific antibody, Conventional antibody, non-conventional antibody, and Single domain antibody. In the literature, there are more than 100 different formats to construct PsAb by quadroma technology, chemical conjugation and genetic engineering. Till March 2023, nine PsAb have been approved around the world, and around 330 are in advanced developmental stages, showing the dominancy of PsAb in the growing health sector. Recent advancements in protein engineering techniques and the fusion of non-conventional antibodies have made it possible to create complex PsAbs that demonstrate higher stability and enhanced potency. This marks the most significant achievement for cancer immunotherapy, in which PsAbs have immense promise. It is worth mentioning that seven out of the nine PsAbs have been approved as anti-cancer therapy. As PsAbs continue to acquire prominence, they could pave the way for the development of novel immunotherapies for multiple diseases.

Symbiotic seed germination and seedling growth of mycorrhizal fungi in Paphiopedilum hirsutissimun (Lindl.Ex Hook.) Stein from China

ABSTRACT Similar to other orchid species, Paphiopedilum hirsutissimum (Lindl.ex Hook.) Stein, relies on nutrients provided by mycorrhizal fungus for seed germination and seedling development in the wild owing to a lack of endosperm in its seeds. Therefore, obtaining suitable and specialized fungi to enhance seed germination, seedling formation, and further development is considered a powerful tool for orchid seedling propagation, reintroduction, and species conservation. In this study, we investigated the diversity, abundance, and frequency of endophytic fungal strains in the root organs of P. hirsutissimum. One family and five genera of the fungi were isolated and identified through rDNA-ITS sequencing. The ability of isolated fungi to germinate in vitro from the seeds of this species was evaluated, and the development of P. hirsutissimum protocorm has been described. The findings showed that the treatments inoculated with endophytic fungal DYXY033 may successfully support the advanced developmental stage of seedlings up to stage 5. In addition, scanning electron microscopy (SEM) revealed that the mycelium of this strain began to invade from either end of the seeds up to the embryo, extending rapidly from the inside to the outside. Its lengthening resulted in the bursting of the seed coat to form protocorms, which developed into seedlings. The results showed that DYXY033 has a high degree of mycobiont specificity under in vitro symbiotic seed germination conditions and is a representative mycorrhizal fungus with ecological value for the species. In summary, this strain may particularly be significant for the protection of P. hirsutissimum species that are endangered in China. In the long run, it may also contribute to global efforts in reintroducing orchid species and in realizing in situ restorations of threatened orchid populations.

Nursery areas of Micromesistius poutassou and Sardina pilchardus unveil their reproductive strategies in the northwestern Mediterranean Sea

AbstractWinter conditions in the NW Mediterranean cause instability of the water column and non‐geostrophic dynamics, such as vertical mixing and convection are significant. These events involve nutrient supply to the photic zone that can sustain high productivity. In this study, we aim to investigate the role of winter hydrodynamics on the spawning strategies of Sardina pilchardus and Micromesistius poutassou. Data were obtained on two oceanographic cruises (February 2017 and 2018) off the Catalan coast. The occurrence of S. pilchardus eggs very close to the coast indicated a clear preference of the species for spawning in coastal areas. Preflexion and postflexion larvae exhibited a slightly wider distribution showing a clear association with the cold, less saline and more productive coastal waters. Preflexion larvae of M. poutassou were found on the upper slope and over the shelf, being offshore limited by the shelf/slope front present all along the slope. The front would act as a barrier preventing their dispersion towards the open sea. M. poutassou larvae in advanced developmental stages were located close to the coast in the productive shelf waters, with instabilities of the front contributing to larval transport from offshore waters to the coast. The vertical distribution of both species showed high variability, not only related with the daily cycle or developmental stage, but also with the vertical structure of the water column. Overall, the results provide some clues on how the spawning strategies of both species may evolve under future scenarios of higher winter‐stratification, because of the global warming.

Integrated assessment of biological responses to pollution in wild mussels (Mytilus edulis) from subarctic and arctic areas in the Norwegian sea

North Atlantic and Arctic Oceans contain large amount of undiscovered oil and gas reserves. Therefore threat of oil spills and its hazardous ecological consequences are of great importance to the marine environment. Although mussels (Mytilus sp.) respond clearly to contaminants, biomarkers have shown variability linked to biological and environmental changes. In order to help avoiding misinterpretation of biological responses the aim of this study was to reveal the effect of natural variability in the responsiveness to pollution of a battery of cell and tissue-level biomarkers in mussels. Mussels were collected in relatively non-impacted and potentially impacted sites at ports and the vicinity of a waste water treatment plant in Trondheim and Tromsø in autumn of 2016. Although the battery of biomarkers used herein proved to be useful to discriminate impacted and non-impacted mussel populations, some confounding factors altering the biological responses were identified. Geographical/latitudinal factors seemed to be critical regarding the reproductive cycle, reserve material storage and the prevalence of parasites such as Gymnophallus cf. Bursicola trematodes. Mussels from the reference site in Tromsø displayed general stress responses at different levels, which could be influenced by the pathogenic effect of the Gymnophallus cf. Bursicola trematode and by a more advanced gametogenic developmental stage compared to the mussels from Trondheim, which could lead to misinterpretation of the reasons behind the measured stress levels in those mussels. Despite these confounding effects, the use of integrative tools such as IBR index helped to discriminate mussel populations from chemically impacted and non-impacted sites. Overall, this work serves as an anchor point both as a reference of the baseline level values of the analyzed endpoints in the studied geographical area and time of the year, and as an indication of the potential extent of the environmental confounding factors in monitoring programs causing stress on the analyzed mussel populations.

Interpreting the results of explicit and applied theory of mind collectively in autistic children: A solution from Rasch analysis.

Theory of mind is an ability to infer others' mental states, which is a foundation for generating appropriate social responses. Theory of mind can be conceptually divided into two related but distinguishable constructs: explicit theory of mind (conceptual knowledge/information about others' mental states) and applied theory of mind (the ability to use theory of mind skills in real-life contexts). Although these two theory of mind scores can be described by the percentages of children in the early, basic, and advanced developmental stages, the resulting information may not be sufficient to determine the corresponding relationships between these two theory of mind constructs or identify children with mismatched theory of mind abilities (e.g. children who have difficulty in effectively applying their theory of mind knowledge in real-life contexts). To resolve these limitations, methods for simultaneously interpreting the relationships between the two theory of mind scores are proposed. Based on the findings, each applied theory of mind score can reflect multiple scores of explicit theory of mind. In particular, the results do not take measurement error into consideration, which would make them more ambiguous. Therefore, the scores of applied theory of mind should be interpreted carefully, given that children who have the same applied theory of mind score may actually have high or low explicit theory of mind. Regarding the method for joint interpretation, cutoff scores were selected to identify children who have mismatched theory of mind abilities (high explicit theory of mind with low applied theory of mind or low explicit theory of mind with high applied theory of mind) and determine the priority for interventions.

Chemical and histological characterization of internodes of sugarcane and energy-cane hybrids throughout plant development

Sugarcane and energy-cane are important Saccharum spp. hybrids that encompass desirable features for energy production. Their stem lignocellulose can be used as the raw material for the second-generation ethanol industry, contributing to accelerated fossil-fuel replacement. However, knowledge on the internode composition and saccharification of these hybrids is still needed to optimize their uses, especially in energy-cane. In this work, the agronomical, chemical, and histological internode features and saccharification potential were analyzed in sugarcane and energy-cane hybrids across four critical developmental stages (tillering, grand growth, early ripening and late ripening) and several internode positions along the stem. Except for galactose content that was higher in the sugarcane hybrid compared to the energy-cane, cell-wall composition was fairly similar in both types of cane across the growing cycle. Among main cell-wall components, maximum crystalline cellulose and matrix polysaccharides contents were observed at tillering and late ripening, respectively. Lignin concentrated towards upper internode positions and advanced developmental stages. Saccharification potential augmented towards apical internodes and early developmental stages, and negatively correlated to lignin and xylose contents. The energy-cane hybrid showed lower number of vascular bundles and metaxylem diameter and its lignin deposition was more extended towards the parenchymal tissue, when compared to the commercial sugarcane throughout the growth cycle. Taken together, these findings demonstrate that developmental stage and internode position largely affect cell-wall composition and its recalcitrance to enzymatic degradation. The use of sugarcane/energy-cane lignocellulose from early developmental stages could be beneficial for the second generation ethanol production. Novel management practices for that usage should consider economic, social and environmental sustainability standards under a sugar/ethanol production scheme.

Social recapitulation: Moulting can restore social tolerance in aggressive spiderlings.

In many taxa, the subsocial route is considered the main pathway to permanent sociality but the relative contribution of offspring interactions and parental care to the maintenance of cohesion and tolerance at advanced developmental stages remains poorly studied. Spiders are relevant models for this question because all exhibit a transient gregarious phase prior to dispersal and permanent sociality, which involves about 20 of the 50,000 species, is assumed to rely on the subsocial route. Using spiderlings of the solitary species Agelena labyrinthica, we manipulated the social context to demonstrate that tolerance in aggressive juveniles can be restored when exposed to siblings after moulting. We propose that moulting can reopen closed critical periods and renew the imprinting to social cues and thus lead to the reacquisition of tolerance. Our study highlights the critical role of contacts between juveniles in the expression of tolerance, which opens novel avenues for understanding social transitions.

Switching ubiquitous and muscle-specific isoforms of mitochondrial respiratory complex IV in skeletal muscle fine-tunes complex IV activity.

Respiratory complex IV (CIV, cytochrome c oxidase) is the terminal enzyme of the mitochondrial electron transport chain. Some CIV subunits have two or more isoforms, which are ubiquitously expressed or are expressed in specific tissues like the lung, muscle, and testis. Among the tissue-specific CIV isoforms, the muscle-specific isoforms are expressed in adult cardiac and skeletal muscles. To date, the physiological and biochemical association between the muscle-specific CIV isoforms and aerobic respiration in muscles remains unclear. In this study, we profiled the CIV organization and expression pattern of muscle-specific CIV isoforms in different mouse muscle tissues. We found extensive CIV-containing supramolecular organization in murine musculature at advanced developmental stages, while a switch in the expression from ubiquitous to muscle-specific isoforms of CIV was also detected. Such a switch was confirmed during the in vitro differentiation of mouse C2C12 myoblasts. Unexpectedly, a CIV expression decrease was observed during C2C12 differentiation, which was probably due to a small increase in the expression of muscle-specific isoforms coupled with a dramatic decrease in the ubiquitous isoforms. We also found that the enzymatic activity of CIV containing the muscle-specific isoform COX6A2 was higher than that with COX6A1 in engineered HEK293T cells. Overall, our results indicate that switching the expression from ubiquitous to muscle-specific CIV isoforms is indispensable for optimized oxidative phosphorylation in mature skeletal muscles. We also note that the in vitro C2C12 differentiation model is not suitable for the study of muscular aerobic respiration due to insufficient expression of muscle-specific CIV isoforms.

The Interrelation between Philosophy for Children (P4C) and Creative Thinking

The aim of teaching philosophy to children ultimately is not to teach them the history of philosophy, but rather to teach them to think, starting from philosophical concepts. This will help to develop high-level skills in children such as questioning, research, understanding and interpreting knowledge, establishing meaningful relationships between knowledge, creating original ideas, and problem-solving. Models of philosophizing with children have been developed, and systematic application attempts have been made, producing successful results in many countries of the world. According to Matthew Lipman, these skills should be developed at a very young age with use of the model of philosophy for children (P4C). Our aim is to show and explore how to develop and sustain creative thinking, which is one of the achievements of P4C. If Lipman is right, children participating in these activities will start to gain creative thinking skills from an early age and will apply this foundation in other knowledge acquisition processes at more advanced developmental stages. This presentation aims to reveal the interrelation between P4C and creative thinking activity. First P4C will be explained, and then creative thinking and the interrelation between the two will be expanded upon in the conclusion.

Spatial distribution of Munida gregaria (Decapoda, Munididae) larvae in the silled Beagle Channel: Insights from spring and autumn surveys

The squat lobster Munida gregaria is an ecologically relevant species in southern South American waters. Nonetheless, fundamental topics regarding its larval ecology remain poorly studied, especially at the southern limit of its distribution area. Here, we investigated the abundance, size, and spatial distribution of M. gregaria larval stages (zoeae I-V and megalopa) in the Beagle Channel, during spring and autumn, and explored their relationships with variations in bathymetry and seawater physico-chemical properties at both sides of the Mackinlay pass sill. Plankton samples were collected from the inner sector of the channel, a fresh water-influenced and semi-enclosed system, to its mouth towards the Atlantic Ocean during four oceanographic cruises (springs of 2014 and 2015 and autumns of 2016 and 2017). Zoeae occurred throughout the study area in both seasons, with lower densities in autumn, whereas megalopae were detected exclusively in spring. The highest larval abundances and all developmental stages were found in the outer sector, where juveniles and adults also occurred. These spatial patterns were consistently observed across the different sampling dates. Contrary to previous reports, the coexistence of zoeae I-V during autumn would indicate that, at least in the outer sector, larvae that hatch late in the summer are able to reach advanced developmental stages. However, it remains unknown whether they fully develop in subsequent months. Significant associations among larval distribution patterns and environmental variables were detected, especially for late stages, which occurred mainly in the westernmost and shallowest part of the outer sector, characterized by highly stratified waters with the warmest bottom temperatures. All together, these results may reflect the effects of variations in local environmental conditions along the channel, related to bottom topography and degree of freshwater/oceanic influence, on larval cohort dynamics, adult reproduction, or both. Larvae hatched in spring were larger than those hatched in summer/autumn for all zoeal stages. Possible explanations for this finding are discussed. This study contributes to our knowledge of M. gregaria larval ecology, which is essential to understand its complex population dynamics in an environmentally complex geographical area, and to better predict its responses to climate change. • Zoeal stages I-V occurred in both spring and autumn. • Larval abundance and stage structure differed west and east of the Mackinlay sill. • Higher larval abundances and all larval stages occurred in the outer sector. • Advanced stages occurred mainly in shallow and highly stratified waters. • Larvae hatched in spring were larger than those hatched in summer-autumn.

The beneficial effect of leaf removal during fruit set on physiological, biochemical, and qualitative indices and volatile organic compound profile of the Cypriot reference cultivar 'Xynisteri'.

'Xynisteri' is the reference Cypriot white cultivar that, despite its significant societal and economic impact, is poorly characterized regarding its qualitative properties, while scarce information exists regarding its aroma profile. In the current study, the effect of leaf removal during fruit set (BBCH 71) on 6-year cordon-trained, spur-pruned grapevines was assessed and an array of physiological, biochemical, and qualitative indices were monitored during successive developmental stages (BBCH 75, BBCH 85, BBCH 87, and BBCH 89). Grapes were additionally monitored for the volatile organic compounds (VOCs) profile during the advanced on-vine developmental stages (BBCH 85-BBCH 89) with the employment of gas chromatography-mass spectrometry (GC-MS), Fourier-transform near infrared (FT-NIR) spectra and electronic nose (E-nose) techniques. Grape berries from the vines subjected to leaf removal were characterized by higher solid soluble sugars (SSC), titratable acidity (TA), tartaric acid, and ammonium nitrogen contents, while this was not the case for assimilable amino nitrogen (primary amino nitrogen). A total of 75 compounds were identified and quantified, including aliphatic alcohols, benzenic compounds, phenols, vanillins, monoterpenes, and C13 -norisoprenoids. Leaf removal led to enhanced amounts of glycosylated aroma compounds, mainly monoterpenes, and C13 -norisoprenoids. Chemometric analysis, used through FT-NIR and E-nose, showed that the aromatic patterns detected were well associated to the grape ripening trend and differences between leaf removal-treated and control grapes were detectable during fully ripe stage. Leaf removal at fruit set resulted in an overall induction of secondary metabolism, with special reference to glycosylated aroma compounds, namely monoterpenes and C13 -norisoprenoids. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Development and long-term dynamics of old-growth beech-fir forests in the Pyrenees: Evidence from dendroecology and dynamic vegetation modelling

Ecological knowledge on long-term forest dynamics and development has been primarily derived from the study of old-growth forests. Centuries of forest management have decreased the extent of temperate old-growth forests in Europe and altered managed forests. Disentangling the effects of past human disturbances and climate on current species composition is crucial for understanding the long-term development of forests under global change. In this study, we investigated disturbance and recruitment dynamics in two forests in the Western Pyrenees (Spain) with contrasting management history: an old-growth forest and a long-untouched forest, both dominated by the two shade-tolerant species Fagus sylvatica (European beech) and Abies alba (Silver fir). We used dendroecological methods in seven plots to analyse forest structure, growth patterns and disturbance histories in these forests. We benchmarked these data with the dynamic vegetation model ForClim to examine the effects of natural and human-induced disturbances on forest development, structure and species composition. Disturbance regimes differed between the study forests, but none showed evidence of stand replacing disturbances, either natural or human induced. Low disturbance rates and continuous recruitment of beech and fir dominated the old-growth forest over the last 400 years. In contrast, the long-untouched forest was intensively disturbed in 1700–1780, probably by logging, with lower natural disturbance rates thereafter. Beech and fir recruitment preferentially occurred after more intense disturbances, despite the high shade tolerance of both beech and fir. Higher fir abundance in the long-untouched forest than in the old-growth forest appeared to be related to its human-induced disturbances. ForClim closely simulated forest potential natural vegetation with a dominance of beech over fir, but overestimated the presence of less shade-tolerant species. Previously observed local fir decline may result from natural forest successional processes after logging. Within ∼200 years after logging cessation, some long-untouched forest structural attributes converged towards old-growth forest, but legacy effects still affected species composition and structure. Natural disturbance regimes in beech-fir forests of the Western Pyrenees induce temporal fluctuations between beech and fir abundance, with a natural tendency for beech dominance in advanced developmental stages with low disturbance rates.

An EvoDevo Study of Salmonid Visual Opsin Dynamics and Photopigment Spectral Sensitivity.

Salmonids are ideal models as many species follow a distinct developmental program from demersal eggs and a large yolk sac to hatching at an advanced developmental stage. Further, these economically important teleosts inhabit both marine- and freshwaters and experience diverse light environments during their life histories. At a genome level, salmonids have undergone a salmonid-specific fourth whole genome duplication event (Ss4R) compared to other teleosts that are already more genetically diverse compared to many non-teleost vertebrates. Thus, salmonids display phenotypically plastic visual systems that appear to be closely related to their anadromous migration patterns. This is most likely due to a complex interplay between their larger, more gene-rich genomes and broad spectrally enriched habitats; however, the molecular basis and functional consequences for such diversity is not fully understood. This study used advances in genome sequencing to identify the repertoire and genome organization of visual opsin genes (those primarily expressed in retinal photoreceptors) from six different salmonids [Atlantic salmon (Salmo salar), brown trout (Salmo trutta), Chinook salmon (Oncorhynchus tshawytcha), coho salmon (Oncorhynchus kisutch), rainbow trout (Oncorhynchus mykiss), and sockeye salmon (Oncorhynchus nerka)] compared to the northern pike (Esox lucius), a closely related non-salmonid species. Results identified multiple orthologues for all five visual opsin classes, except for presence of a single short-wavelength-sensitive-2 opsin gene. Several visual opsin genes were not retained after the Ss4R duplication event, which is consistent with the concept of salmonid rediploidization. Developmentally, transcriptomic analyzes of Atlantic salmon revealed differential expression within each opsin class, with two of the long-wavelength-sensitive opsins not being expressed before first feeding. Also, early opsin expression in the retina was located centrally, expanding dorsally and ventrally as eye development progressed, with rod opsin being the dominant visual opsin post-hatching. Modeling by spectral tuning analysis and atomistic molecular simulation, predicted the greatest variation in the spectral peak of absorbance to be within the Rh2 class, with a ∼40 nm difference in λmax values between the four medium-wavelength-sensitive photopigments. Overall, it appears that opsin duplication and expression, and their respective spectral tuning profiles, evolved to maximize specialist color vision throughout an anadromous lifecycle, with some visual opsin genes being lost to tailor marine-based vision.

A Structural Assessment of Sycamore Maple Bark Disintegration by Nectria cinnabarina

Previous phytopathological studies of the fungal pathogen Nectria cinnabarina have been focused on its distribution and host diversity but little is known about the spread of this pathogen and the defence responses of forest trees to an infection inside host tissues. Histopathological alterations of bark, periderm, phloem and woody tissues were investigated in sycamore maple (Acer pseudoplatanus) branches following their natural attack by the advanced anamorph and teleomorph developmental stages of the fungus. Light, fluorescence, confocal laser scanning and scanning electron microscopy techniques supplemented by X-ray micro-computed tomography imaging were used to distinguish between healthy and disintegrated plant tissues. The intercellular spread of fungal hyphae was found primarily in the phelloderm. Expanding hyphae aggregations produced ruptures in the phellem and the disintegration of both phellogen and phellodermal parenchyma cells in close proximity to the expanding fruiting bodies of the fungus. Thicker hyphae of the teleomorph fungal stage heavily disintegrated the phelloderm tissues and also induced enhanced sclerification of the nearby phloem tissues that limited the spread of the infection into the sieve tubes. Both the intercellular and intracellular spread of hyphae inside the peripheral parts of sclereid clusters led to the disintegration of the compound middle lamellae but the hyphae were only rarely able to pass through these structural phloem barriers. The massive fungal colonization of both lumens and disintegrated tangential cell walls of ray parenchyma cells resulted in severe cambial necroses. Although the hyphae penetrated into the outermost annual growth rings of the xylem, no cell wall disintegration of the parenchyma cells, vessels and fibres was revealed. Despite the local cambial necroses and severe phloem ray disintegration, the bark remained attached to the examined branches and no bark cankers were formed.

The Future of Precision Medicine to Predict Outcomes and Control Tissue Remodeling in Inflammatory Bowel Disease

Inflammatory bowel disease is characterized by significant interindividual heterogeneity. With a wider selection of pharmacologic and nonpharmacologic interventions available and in advanced developmental stages, a priority for the coming decade is to determine accurate methods of predicting treatment response and disease course. Precision medicine strategies will allow tailoring of preventative and therapeutic decisions to individual patient needs. In this review, we consider the future of precision medicine in inflammatory bowel disease. We discuss the critical need to extend from research focused on short-term symptomatic response to integrative multi-omic systems biology strategies to identify and validate biomarkers that underpin precision approaches. Crucially, the international community has collective responsibility to provide well-phenotyped and -curated longitudinal datasets for scientific discovery and validation. Research must also study broader aspects of the immune response, including components of the extracellular matrix, to better understand biological pathways initiating and perpetuating tissue fibrosis and longer-term disease complications.

Nutritional Composition of Honey Bee Drones of Two Subspecies Relative to Their Pupal Developmental Stages.

Simple SummaryDespite the use of honey bee brood as food among several communities of the world, the nutritional potential of drones remained unexplored for a long time. In the recent past some scientific endeavour, including our own previous work, has been undertaken to explore the nutrient quality of this food source. Due to their limited socio-biological role, honey bee drones would be a suitable candidate to compare their nutrient content with that of worker honey bees. We therefore investigated the nutrient composition of honey bee drones belonging to two subspecies, namely Apis mellifera carnica and A. m. mellifera covering their pupal developmental period. To possess information of the drones’ nutritional value during their development would help in choosing the most suitable developmental stage for the commercial production of drone brood as food.We examined the contents of nutritional importance, i.e., amino acids, fatty acids and minerals of different developmental stages of drones of two honey bee subspecies, namely Apis mellifera carnica and A. m. mellifera. The results revealed that, in general, individual amino acid amounts and therefore the total protein increased along with the developmental stages of the drones. No statistically significant differences were found between the same developmental stages of the two subspecies. The reverse, i.e., a decrease with developmental stage occurred in relation to the fatty acid composition. Most of the minerals were higher at advanced developmental stages. Overall, the high protein content (31.4–43.4%), small amount of fat (9.5–11.5%) and abundance of minerals such asiron and zinc, make drones a suitable nutritional resource. Even though nutrient content, especially protein, was higher in the pupae than the prepupae, we propose prepupae also as a commercial product based on their higher biomass production. Provided standard production protocols maintaining hygiene and safety will be adhered to, we propose that drone honey bees can be utilized as human food or animal feed.

Gender and Police Leadership: An Analysis of Metropolitan Police Departments in South Africa

Despite a number of initiatives aimed at improving the representation and progressive of women in the law enforcement. Studies continue to document the persistence of gender inequality within law enforcement agencies all over the world and South Africa is not an exception. This article bring to light gender inequalities in the law enforcement sector where women in leadership ranks remains low. Historically, the police career was male-dominated and females were not allowed to work in the police. Therefore, equal gender representation in the workplace should by now be at an advanced developmental stage in South Africa since the abolition of discrimination rules. The Commission on Employment Equity of South Africa reported that women comprised 44.8% of the economically active population, yet males were still in charge of senior management positions in South African industries including the law enforcement environment. The data was collected from female officers from Metropolitan Police departments in Gauteng province. The findings suggest that culture, stereotypes, economic and socio-political dynamics, and physical fitness were perceived as barriers that hindered the representation of women into senior leadership positions.

O-213 Slow day 5 development affects implantation potential of fresh transferred embryos but not birthweight once pregnancy occurs: A multi-center retrospective cohort study

Abstract Study question Does slow development of fresh transferred day 5 embryos lead to decreased implantation potential and birthweight? Summary answer Slow day 5 development was associated with reduced implantation potential when transferred fresh but the subsequent birthweight of the resulting baby was not impacted. What is known already Slow development of in vitro cultured cleavage stage embryos is associated with reduced blastocyst development and implantation rates. There is no current consensus regarding whether to transfer fresh slow developing day 5 embryos or to extend culture for a subsequent day with potential for cryopreservation. It is therefore important to understand the true prognosis of fresh transferred day 5 embryos at less advanced developmental stages. This would provide evidence based guidelines for the decision making process in regard to embryo transfer. Study design, size, duration This is a retrospective multi-center cohort study, including 1213 consecutive patients undergoing autologous oocyte in vitro fertilization (IVF) treatment during 2016-2019,with fresh transfer of a single day 5 embryo (selection based on developmental stage and inner cell mass and trophectoderm morphology if blastocyst was at the ≥expanding stage). Cycle data were collected from 4 associated private clinics, with repeat cycles of same patients excluded to avoid clustering effect at statistical analysis. Participants/materials, setting, methods Live birth and birthweight were followed up in all 1213 fresh day 5 SETs. Multiple regression (logistic or linear) was performed to investigate association between slow day 5 development (defined as ≤ early blastocyst) and (a)live birth, (b) birthweight, and (c) gestation-adjusted birthweight (Z score) to account for gestational age, gender and compared to embryos at ≥ expanded stage. Results were expressed as adjusted odds ratio (aOR) with 95% confidence interval (CI)or coefficients (β). Main results and the role of chance No implantation was achieved following single fresh transfer of day 5 embryos that failed to reach early blastocyst stage (n = 76) and were transferred as ≤ morula stage. Live birth rate was significantly lower following single day 5 fresh transfer of an early blastocyst (n = 237, 16%), in comparison to expanding (n = 329, 27%, P = 0.001), expanded(n = 392, 41%, P = 0.000), and hatching/hatched blastocysts (n = 169, 44%, P = 0.000). After adjusting for potential confounding factors including; maternal age, hours post insemination at day 5 assessment, number of oocytes collected, number of 2PN embryos, and number of embryos frozen; multiple logistic regression showed significantly reduced likelihood of live birth resulting from early blastocysts in reference to those at the expanding (aOR=0.584, 0.371-0.917, P = 0.020), expanded (aOR=0.322, 0.208-0.501, P = 0.000), or hatching/hatched stages (aOR=0.255, 0.147-0.443, P = 0.000). However, multivariate linear regression indicated that early blastocysts resulting in a live birth (n = 39) did not lead to altered birthweight (β=-9.091, P = 0.904; β=-34.960, P = 0.343; β=-26.074, P = 0.414; respectively) or Z score (β = 0.045, P = 0.706; β=-0.051, P = 0.426; β=-0.028, P = 0.506; respectively) in reference to the expanding (n = 90), expanded (n = 160), or hatching/hatched stages (n = 75). Limitations, reasons for caution The retrospective nature of this study does not allow controlling of unknown confounders. The 4 participating clinics are associated within the same network with shared protocols, therefore, results may not be generalized to other clinics with different settings. Wider implications of the findings The findings suggest no clinical value of fresh day 5 transfer of embryos ≤morula stage. Although early blastocysts implant at reduced rate, assuring birthweight outcomes suggest clinical value. Future studies intend to investigate slow growing day 5 fresh transfers versus embryos that were slow growing but transferred after day 6. Trial registration number NA