The Aedes aegypti L. mosquito is a vector of diseases such as classic and hemorrhagic dengue (DENV), chikungunya (CHIKV), Zika (ZIKV), yellow fever (YFV), and Mayaro (MAYV). In 2019 alone, in Latin America, there were 2.7 million cases and 1206 deaths. In Mexico, government programs for managing this pest include the elimination of larval habitats and chemical control of larvae and adults. The objective of this study was to evaluate the response of two larvae and adult populations of A. aegypti from the state of Guerrero, Mexico, under laboratory conditions, to insecticides with different modes of action and to make considerations for their management. In the bioassays, larvae showed susceptibility to permethrin, malathion, deltamethrin, and Bacillus thuringiensis var. israelensis; low resistance to Temephos and propoxur; as well as moderate resistance to Spinosad. Both adult populations showed resistance to permethrin and susceptibility to deltamethrin, while the Acapulco population showed resistance to alpha-cypermethrin. For larvae, Spinosad is suggested to be used in times of high incidence of dengue. During drought, it is advisable to include rotations of B. thuringiensis var. israelensis with organophosphates. For adults, the use of imidacloprid in combination with botanical insecticides is considered a rational alternative to rotations with organophosphates and pyrethroids.