Background: Nano-composite polymer from Nano graft co–polymer that has the ability to absorb, but not dissolve in, water at physiological conditions (pH, temperature, mass of adsorbent). research the adsorption systems of drug (vitamin B12 drug) on selected surfaces (Nano-composite co-polymer) at variable conditions of pH and temperature. Methods: The UV-Visible spectrometer was used to conduct the adsorption experiments. FTIR (Fourier transform infrared spectrometer) and AFM were used to study the chemical structures of polymers. Results: When the adsorption phenomenon was investigated as different temperatures (10, 20,35 and 55 oC), it was found that the degree of adsorption of vitamin B12 drug on Nano-composite co-polymer was increased with temperature increase. The amounts of drug that were adsorbed on the Nano-composite co-polymer surface at different pH, were in the following order: 11, 6, 4, 2 and successful removal at pH 11.