Adriamycin In Tissues Research Articles

Determination of adriamycin in plasma and tissue biopsies

A simple, rapid and sensitive method for the extraction and high-performance liquid chromatographic analysis of adriamycin in tissue and plasma is described. Tissue (5-100 mg) and plasma (1 ml) samples underwent a C18 Sep-Pak extraction into methanol. Chromatography was performed on a muBondapakphenyl column using a mobile phase of acetonitrile-0.1 M ammonium formate (pH 4.0) with a flow-rate of 2 ml/min. Fluorometric detection was used with an excitation of 480 nm and an emission of 550 nm. The procedure produced a linear curve for the concentration range 25-1000 ng/ml. The development of the assay produced rapid, repeatable and accurate results for both small tissue samples and plasma.

The delineation of adriamycin extravasation using fluorescence microscopy.

Frozen-section fluorescence of Adriamycin extravasation in skin has been demonstrated to be an efficient method of detecting Adriamycin in tissue. Appropriate use of this technique as a guide to surgical treatment of infiltration is feasible, but it must await clinical trials.

Spectrophotofluorometric characterization of adriamycin a new antitumour drug

Adriamycin, a new antitumour drug structurally similar to daunomycin, shows evident spectrophotofluorometric characteristics. A technique previously employed by Kohn for tetracyclines is proposed by the present workers for the identification and estimation of adriamycin in tissues. Furthermore, a transformation of the molecule was revealed in some organs, using the same technique.