The present study is geared towards the investigation of the impact of fuel temperature, ambient pressure, and fuel properties on the spray characteristics of a 6-hole gasoline direct injector. High speed imaging is employed to verify the spray structural variation of various fuels including iso-octane, gasoline, methanol, and ethanol over wide range of operating conditions in an optical vessel. These structural parameters were correlated to the ratio of the ambient to saturation pressure ratio (Pa/Ps) that represents the superheated degree. At a constant pressure of 150 bar the fuel was injected, and the fuel temperature ranged between 25 and 120 °C, whilst the ambient pressure ranged from 0.1 to 1 bar. The results indicated that for all the fuels, the spray width was reduced, and the adjacent plumes collapsed into single bulk as the (Pa/Ps) decreased. Furthermore, at very low (Pa/Ps = 0.065), the spray getting longer, and for iso-octane the fuel spray shrinkage toward the injector centreline and shaped like a Fish with sharpen spray tip. The spray cone angle for ethanol, methanol, and gasoline was reduced when the Pa/Ps ratio was decreased from 1 to 0.16 with the exception that at Pa/Ps = 0.54 for ethanol the cone angle was increased and then decreased with the reduction of Pa/Ps. Then a massive increase in the cone angle was noticed by reducing the Pa/Ps ratio reduced from 0.16 to 0.065. For iso-octane, the behaviour is chaotic and not follow any specific trend with the reduction of Pa/Ps. Furthermore, the appearance of interstitial streams in the gaps between the original spray streams was noticed at Pa/Ps ratio ≤ 0.28. Phase Doppler data showed for the Pa/Ps ratios of 0.85, 0.54, and 0.28, that the interstellar streams consistently had a lower mean droplets velocity and a very narrow head stage compared to that of spray main streams. Moreover, reducing the Pa/Ps ratios of 0.85, 0.28, resulted in a significant reduction in Sauter mean diameter with approximately 49.5%.