Simple SummaryPenguins are a common zoo-housed species and have been shown to display behaviours indicative of fear such as huddling, vigilance and avoidance towards zoo visitors. However, this evidence has been obtained from a single public zoo in Melbourne, Australia. Therefore, we investigated the effect of covering a visitor viewing area window on fear behaviour of zoo-housed little penguins at another zoo in Sydney, Australia. Covering one out of four visitor viewing area windows reduced the number of visitors and the occurrence of potentially threatening visitor behaviours at this window such as banging on the window, loud vocalisations and sudden movement. When the viewing window was covered, the number of penguins visible and preening in the water increased and the number of penguins vigilant near this viewing window reduced. Also, the adjacent corner area, which was not visible to visitors, was found to be a preferred area for the penguins whether the viewing window was uncovered or covered. While there were limited effects, the reduced presence, reduced preening in the water and increased vigilance by penguins near the viewing window when this window was uncovered, together with the general preference for the corner area, provides evidence of some avoidance of visitors. These results suggest that visual contact with visitors and/or other types of visitor contact, such as visitor-induced sounds and vibrations, may be fear-provoking for zoo-housed little penguins. Therefore, these results suggest that penguins in zoos may benefit from modifications to the enclosure that may ameliorate penguin fear responses to visitors such as one-way viewing glass, barriers reducing close visitor contact and areas for penguins to retreat.Studies on the effects of visitors on zoo animals have shown mixed findings and as a result, the manner in which visitors affect zoo animals remains unclear for many species, including a rarely studied taxa such as penguins. Penguins are a common zoo-housed species and have been shown to display huddling, vigilance and avoidance towards zoo visitors which can be indicative of fear. Here, we examined the effects of covering one visitor viewing area window, out of four, on little penguin (Eudyptula minor) behaviours that may be indicative of fear. Two treatments were randomly imposed on different days: (1) The main visitor viewing area window, where most visitor-penguin interactions occurred, was uncovered (‘Main window uncovered’) and (2) The main visitor viewing area window was covered (‘Main window covered’). Penguin numbers and behaviour were recorded near the main visitor viewing area window and the three other visitor viewing area windows, as well as one area not visible to visitors (‘Corner’ area). Furthermore, visitor numbers and visitor behaviour were recorded at all four visitor viewing area windows. Covering the main visitor viewing area window reduced the proportion of visitors present at this window by about 85% (p < 0.001) and reduced potentially threatening visitor behaviours at this window such as tactile contact with the window, loud vocalisations and sudden movement (p < 0.05). When the main visitor viewing area window was covered, the proportion of penguins present increased by about 25% (p < 0.05), the proportion of visible penguins preening in the water increased by about 180% (p < 0.05) and the proportion of visible penguins vigilant decreased by about 70% (p < 0.05) in the area near this main window. A preference for the Corner area was also found whereby 59% and 49% of penguins were present in this area when the main window was uncovered and covered, respectively. These results provide limited evidence that the little penguins in this exhibit showed an aversion to the area near the main visitor viewing area window when it was uncovered based on the increased avoidance and vigilance and decreased preening in the water in this area. This suggests visitors may be fear-provoking for these little penguins. However, it is unclear whether visual contact with visitors per se or other aspects of visitor contact, such as visitor-induced sounds and vibrations, were responsible for this apparent aversion when this window was uncovered.
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