Adjacent Cell Walls Research Articles

Improving strength and plasticity via pre-assembled dislocation networks in additively manufactured refractory high entropy alloy

Refractory high entropy alloys (RHEAs), as a novel class of multi-principal element alloys, have attracted significant attention owing to their excellent properties. However, their low plasticity limits their potential applications, while the high melting points of the alloying elements face challenges to additive manufacturing (AM). Herein, RHEA, with extensively distributed cellular structure within their grains, was successfully fabricated using AM. Furthermore, we proposed a simple strategy to form a complete dislocation network within the cellular structure region in advance through cyclic deformation processing in the elastic stage (microplastic deformation). Dislocation networks are entangled with other dislocations, creating numerous pinned points adjacent cell walls, which impede dislocation motion. As a result, the cyclic deformation processing of RHEA achieves a yield strength of 1136 MPa while maintaining 50 % deformation strain without fracturing. The cyclic deformation processing method provides a route to strengthen additively manufactured alloys, offering a solution to overcome the trade-off between strength and plasticity.

Numerical study on energy absorption performance of novel bionic helmet liner

In this paper, by introducing the pyramid-shaped spine configuration of durian shells and bidirectional corrugated configuration of jaw feet of mantis shrimps into the helmet liner, a bidirectional staggered rectangular frustum liner was designed. Finite element simulation of impact was carried out on liners with various pyramid-shaped cell structures, and the kinetic behavior of the liners of different structures during impact compression was studied. The results showed that the decrease in the inclination angle of the outer walls of pyramid-shaped cells and the bidirectional-cell design can both improve the specific energy absorption of the helmet, and that the bidirectional staggered rectangular frustum helmet has the highest specific energy absorption, 2.7 times that of the gradient lattice or 1.3 times that of the honeycomb helmet. This is because the bidirectional staggered rectangular frustum cell design enhances the connection between adjacent cell walls, resulting in a larger deformation area during the impact process and participation of more material in energy storage, thus significantly improving the overall energy absorption performance.

Further study on the dynamic loading transmission in cellular solids based on one-dimensional mass-spring model

The characteristics of dynamic loading transmission in cellular solids are studied in the present paper using a one-dimensional (1D) deformation-contact mass-spring model. From the perspective of deformation process, the collisions among adjacent cell walls happen due to the shock-induced localized large deformation and the cellular structure of the material. Two springs representing deformation stress and contact stress, respectively, are introduced to the mass-spring model to represent different deformation and loading transmission mechanisms. Combined with shock-wave model, the contact stress in the densification stage is determined. Both quasi-static and dynamic compression of cellular solids can be described by the 1D mass-spring model, in which the critical impact velocity of the compaction shock is used to distinguish two different compression states. Based on the 1D deformation-contact mass-spring model, several important issues in the dynamic loading transmission in cellular solids, i.e. the deformation-contact process, unloading and reversed loading issue, deformation modes under different loading conditions, stress effectiveness and micro-inertia effect, are examined. The present paper clarifies previous concerns on the mass-spring model and demonstrates its enhanced capability to simulate the dynamic loading transmission in cellular solids.

Analysis of the Distribution of Symplasmic Tracers During Zygotic and Somatic Embryogenesis.

Plasmodesmata (PD) are membraneous channels that span cell walls of adjacent cells to establish the symplasm. These connections are unique to plants and enable the cell-to-cell exchange of information via the symplasm. However, not every plant cell is connected to its neighbor. Absence of PD and lack of communication (symplasmic isolation) are important regulators of cell differentiation. To determine cell-to-cell symplasmic connectivity, the distribution of fluorescent tracers can be analyzed. Here, we describe in detail the entire procedure for conducting such analysis using fluorescence and confocal microscopy to study molecular fluxes in fluorescence recovery after photobleaching (FRAP) experiments. Studies using fluorochromes and fluorescent-labeled dextrans successfully inform the degree of symplasmic connectivity between cells in zygotic and somatic embryos. Small molecules, such as water and ions, travel through PD but also transcription factors and different types of RNA. Studies of symplasmic communication are important to determine the spatio-temporal correlation between cell differentiation and the exchange of information between cells. This information is necessary to determine the role of symplasmic communication during embryogenesis, which is a very important stage in plant development and morphogenesis.

Impact response of a novel sandwich structure with Kirigami modified corrugated core

A novel Kirigami modified corrugated core was proposed recently and its superior crushing performance under static loading was demonstrated. The proposed core is modified from the conventional corrugated core using Kirigami design, where a portion of the corrugated sheet is cut and then folded vertically. The vertical fold-ins of Kirigami modified corrugated core provide extra support and constraints to the adjacent un-folded cell walls, which increase the crushing resistance of a unit cell up to 10 times as previously studied. However, for a panel with multiple unit cells, its longitudinal flexural stiffness and impact resistance could be affected because of the fold-ins. This study investigates its performance and energy absorption under dynamic crushing through pendulum impact tests. Four configurations of panels, including laminated flat plate (FL), conventional corrugated (CC), Kirigami modified corrugated 1 (KC1) and Kirigami modified corrugated 2 (KC2) were tested. Different impact mass, velocities as well as impacting locations were considered. Impact force and rear face centre deflection were measured and compared to evaluate the impact performance of the panels. Superior impact resistance was demonstrated for proposed KC panel over CC panel under all impact scenarios.

Functional packaging of seeds.

SummaryThe encapsulation of seeds in hard coats and fruit walls (pericarp layers) fulfils protective and dispersal functions in many plant families. In angiosperms, packaging structures possess a remarkable range of different morphologies and functionalities, as illustrated by thermo and hygro‐responsive seed pods and appendages, as well as mechanically strong and water‐impermeable shells. Key to these different functionalities are characteristic structural arrangements and chemical modifications of the underlying sclerenchymatous tissues. Although many ecological aspects of hard seed encapsulation have been well documented, a detailed understanding of the relationship between tissue structure and function only recently started to emerge, especially in the context of environmentally driven fruit opening and seed dispersal (responsive encapsulations) and the outstanding durability of some seed coats and indehiscent fruits (static encapsulations). In this review, we focus on the tissue properties of these two systems, with particular consideration of water interactions, mechanical resistance, and force generation. Common principles, as well as unique adaptations, are discussed in different plant species. Understanding how plants integrate a broad range of functions and properties for seed protection during storage and dispersal plays a central role for seed conservation, population dynamics, and plant‐based material developments.

3D Dissection of Structural Membrane-Wall Contacts in Filamentous Moss Protonemata.

In conventional light microscopy, the adjacent cell walls of filamentous moss protonemata are seen from its narrow side thereby obscuring the major area of cell–cell connection. Optical sectioning, segmentation and 3D reconstructions allow the tilting and rotation of intracellular structures thereby greatly improving our understanding of interaction between organelles, membranes and the cell wall. Often, the findings also allow for conclusions on the respective functions. The moss Physcomitrium (Physcomitrella) patens is a model organism for growth, development and morphogenesis. Its filamentous protonemata are ideal objects for microscopy. Here, we investigated the cell wall between two neighboring cells and the connection of membranes towards this wall after plasmolysis in 0.8 M mannitol. An m-green fluorescent protein (GFP)-HDEL cell line was used to visualize the endoplasmatic reticulum (ER), the plasma membrane (PM) was stained with FM4-64. Our studies clearly show the importance of cell–cell contacts in P. patens protonemata. In 86% of the investigated cell pairs, at least one of the protoplasts remained fully attached to the adjacent cell wall. By tilting of z-stacks, volume renderings and 3D reconstructions, we visualized the amount of attached/detached PM and ER components after plasmolysis and membrane piercings through the wall of cell neighbors.

Sorption behavior and hydroxyl accessibility of wood treated with different cyclic N-methylol compounds

Cyclic N-methylol compounds such as 1,3-dimethylol-4,5-dihydroxyethyleneurea (DMDHEU) have been used to modify wood and prevent negative effects related to the uptake of moisture. However, the changes in the sorption behavior of wood by treatments with DMDHEU and its derivatives are not fully understood. In the present study, wood blocks were treated with DMDHEU, ether-modified DMDHEU and diethyleneglycolated DMDHEU in order to study the factors that control the changes in moisture uptake in the hygroscopic range (0–95% RH). Dimensional changes of wood blocks during water soaking cycles suggested that the treatments caused a permanent cell wall bulking, whereas the swelling restraint by cross-linking of adjacent cell wall polymers was not permanent. However, the changes in water vapor sorption were not only a result of the cell wall bulking effect that reduced the space in the cell wall to accommodate water. The N-methylol compounds within the wood also provided additional sorption sites, but there was no correlation between absorbed water and accessible OH groups. It was speculated that the co-condensation of the N-methylol compounds with wood polymers had a significant effect on the sorption of the treated wood. At elevated RH, pure resins that were formed by self-condensation took up large quantities of moisture. However, when the N-methylol compounds were heat-cured within the hierarchical structure of wood, the moisture uptake of the treated wood at elevated RH was even lower compared to unmodified wood. Furthermore, the covalent bond formation between wood and resin prolonged the attainment of an equilibrium moisture content.

Macroscopic fossil charcoals as proxy of a local fire linked to conifer-rich forest from the late Pliocene of northwestern Yunnan, Southwest China

Yunnan at southeastern margin of the Tibetan Plateau is subject to frequent wildfires each year, while its wildfire history remains poorly known due to the lack of studies on palaeofire in the region. In this study, we report a local fire from the late Pliocene of northwestern Yunnan, based on macroscopic fossil charcoals recovered from the Sanying Formation of Lanping Basin. These sedimentary charcoals exhibit silky lustre in the light and complete homogenization of adjacent xylem cell walls, characterizing the result of incomplete combustion during the late Pliocene. Our preliminary taxonomic analysis indicates that the studied charcoals are dominated by conifers, suggesting higher importance of coniferous elements as fuel sources in the fire. We assert a conifer-rich source forest for the fire event by also considering plant remains of other types, i.e., needle fragments, small shoots, fruits and seeds, from the same sampling layer. Since conifers are commonly prone to wildfires, this type of forest might have a close link with the fire by serving highly flammable fuels. We consider that the regionally seasonal drought during the late Pliocene might also take responsibility, because in the dry season forest fuels such as ground litter would become ignitable after intensive desiccation. As modern wildfires in northwestern Yunnan are closely coupled with conifer-dominant forests and seasonally dry climate, we assume this correlation might have been established by the late Pliocene. Our study may bring attention to potential roles of wildfire on local and/or regional flora and vegetation evolution in this region.

Thermospermine Synthase (ACL5) and Diamine Oxidase (DAO) Expression Is Needed for Zygotic Embryogenesis and Vascular Development in Scots Pine.

Unlike in flowering plants, the detailed roles of the enzymes in the polyamine (PA) pathway in conifers are poorly known. We explored the sequence conservation of the PA biosynthetic genes and diamine oxidase (DAO) in conifers and flowering plants to reveal the potential functional diversification of the enzymes between the plant lineages. The expression of the genes showing different selective constraints was studied in Scots pine zygotic embryogenesis and early seedling development. We found that the arginine decarboxylase pathway is strongly preferred in putrescine production in the Scots pine as well as generally in conifers and that the reduced use of ornithine decarboxylase (ODC) has led to relaxed purifying selection in ODC genes. Thermospermine synthase (ACL5) genes evolve under strong purifying selection in conifers and the DAO gene is also highly conserved in pines. In developing Scots pine seeds, the expression of both ACL5 and DAO increased as embryogenesis proceeded. Strong ACL5 expression was present in the procambial cells of the embryo and in the megagametophyte cells destined to die via morphologically necrotic cell death. Thus, the high sequence conservation of ACL5 genes in conifers may indicate the necessity of ACL5 for both embryogenesis and vascular development. Moreover, the result suggests the involvement of ACL5 in morphologically necrotic cell death and supports the view of the genetic regulation of necrosis in Scots pine embryogenesis and in plant development. DAO transcripts were located close to the cell walls and between the walls of adjacent cells in Scots pine zygotic embryos and in the roots of young seedlings. We propose that DAO, in addition to the role in Put oxidation for providing H2O2 during the cell-wall structural processes, may also participate in cell-to-cell communication at the mRNA level. To conclude, our findings indicate that the PA pathway of Scots pines possesses several special functional characteristics which differ from those of flowering plants.

De-Esterified Homogalacturonan Enrichment of the Cell Wall Region Adjoining the Preprophase Cortical Cytoplasmic Zone in Some Protodermal Cell Types of Three Land Plants.

The distribution of highly de-esterified homogalacturonans (HGs) in dividing protodermal cells of the monocotyledon Zea mays, the dicotyledon Vigna sinensis, and the fern Asplenium nidus was investigated in order to examine whether the cell wall region adjoining the preprophase band (PPB) is locally diversified. Application of immunofluorescence revealed that de-esterified HGs were accumulated selectively in the cell wall adjacent to the PPB in: (a) symmetrically dividing cells of stomatal rows of Z. mays, (b) the asymmetrically dividing protodermal cells of Z. mays, (c) the symmetrically dividing guard cell mother cells (GMCs) of Z. mays and V. sinensis, and (d) the symmetrically dividing protodermal cells of A. nidus. A common feature of the above cell types is that the cell division plane is defined by extrinsic cues. The presented data suggest that the PPB cortical zone-plasmalemma and the adjacent cell wall region function in a coordinated fashion in the determination/accomplishment of the cell division plane, behaving as a continuum. The de-esterified HGs, among other possible functions, might be involved in the perception and the transduction of the extrinsic cues determining cell division plane in the examined cells.

Fabrication and mechanical behaviors of an all-composite sandwich structure with a hexagon honeycomb core based on the tailor-folding approach

The tailor-folding method is proposed to make an all-composite sandwich panel with a carbon fiber reinforced polymer (CFRP) hexagon honeycomb core. Using this method, a CFRP honeycomb core with continuous fibers is fabricated automatically from a continuous plain woven prepreg to reinforce the constraints between adjacent cell walls. The analytical expressions were derived for predicting the stiffness and strength of the CFRP hexagon honeycomb sandwich panels for out-of-plane compression and shear loadings. The corresponding failure mechanism maps were also constructed for estimating the dominant failure mode of the honeycomb core, including elastic buckling and fracture under out-of-plane compressive and shear loadings. Selected geometries of the sandwich panels were tested to illustrate these failure modes, with a reasonable agreement between the analytical predictions and experiments. It was observed that the CFRP hexagon honeycomb exhibit good specific energy absorption ability. The tailor-folding method has the potential to provide new opportunities for lightweight multifunctional honeycombs.

A simplified thermomechanical approach to visualize hexagonal honeycomb construction

The construction of honeycombs astonished human being from ancient time due to their perfect geometrical structure. How the hexagonal cells are constructed from fresh circular cells during the cell building process is a matter of long debate. Here we show solely from the thermomechanical properties of self-synthesized beeswax how the honeybees permanently transform the fresh circular cells to rounded hexagons by creating a temperature gradient in the vicinity of the triple junctions of circular cell walls. By assuming conventional Fourier’s law of heat conduction and mechanical properties of beeswax, we show via computer simulations that with increasing temperature gradient, the stress on the adjacent circular cell walls increases due to enhanced fusion, leading to the deformation from the original positions of the circular cell walls. The calculated von Mises stresses are found to be significantly larger than the yield stress, indicating the permanent deformation of the circular cell walls to rounded hexagonal shape. This suggests that it is the thermomechanical properties of the building material for which the comb cells take rounded hexagonal shapes.

Plant-Derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications.

Despite considerable recent interest in micro- and nanofibrillated cellulose as constituents of lightweight structures and scaffolds for applications that range from thermal insulation to filtration, few systematic studies have been reported to date on structure-property-processing correlations in freeze-cast chitosan-nanocellulose composite scaffolds, in general, and their application in tissue regeneration, in particular. Reported in this study are the effects of the addition of plant-derived nanocellulose fibrils (CNF), crystals (CNCs), or a blend of the two (CNB) to the biopolymer chitosan on the structure and properties of the resulting composites. Chitosan-nanocellulose composite scaffolds were freeze-cast at 10 and 1 °C/min, and their microstructures were quantified in both the dry and fully hydrated states using scanning electron and confocal microscopy, respectively. The modulus, yield strength, and toughness (work to 60% strain) were determined in compression parallel and the modulus also perpendicular to the freezing direction to quantify anisotropy. Observed were the preferential alignments of CNCs and/or fibrils parallel to the freezing direction. Additionally, observed was the self-assembly of the nanocellulose into microstruts and microbridges between adjacent cell walls (lamellae), features that affected the mechanical properties of the scaffolds. When freeze-cast at 1 °C/min, chitosan-CNF scaffolds had the highest modulus, yield strength, toughness, and smallest anisotropy ratio, followed by chitosan and the composites made with the nanocellulose blend, and that with crystalline cellulose. These results illustrate that the nanocellulose additions homogenize the mechanical properties of the scaffold through cell-wall material self-assembly, on the one hand, and add architectural features such as bridges and pillars, on the other. The latter transfer loads and enable the scaffolds to resist deformation also perpendicular to the freezing direction. The observed property profile and the materials' proven biocompatibility highlight the promise of chitosan-nanocellulose composites for a large range of applications, including those for biomedical implants and devices.

Морфологічні особливості насінин деяких видів роду Epipactis Zinn. (Orchidaceae Juss.) флори України

The investigation of macro- and micromorphological peculiarities of seeds of four species of genus Epipactis (Orchidaceae) of Ukrainian flora were carried out. The genus Epipactis is difficult in the in in taxonomic terms and for its representatives are characterized by polymorphism of morphological features of vegetative and generative organs of plants and ability of species to hybridize. The aim of the research was to perform a comparative morphological study of seeds of E. helleborine, E. albensis, E. palustris, E. purpurata and to determine carpological features that could more accurately identify species at the stage of fruiting. A high degree of variation in the shape of the seeds in different populations within the species and overlap of most quantitative carpological characteristics of studied species are noted. There were no significant differences in micromorphological features of the structure of the testa at species or population level. The reticulate surface of the testa is characteristic of all species, the cells of testa are mostly elongated, penta-hexagonal, individual cells almost isodiametric-pentagonal. From the micropillary to the chalasal end, a noticeable change in the shape and size of the seed coat cells is not observed. There are no intercellular spaces, the anticlinal walls of adjacent cells are intergrown and the boundaries between them become invisible. The outer periclinal walls have a single, mainly longitudinal thin ribbed thickenings. Anticlinal cell walls are thick, dense, smooth. The longitudinal Anticlinal walls are almost straight, transverse - straight or sometimes curved in some cells. Epicuticular deposits on the periclinal walls are absent. It is concluded that the use of macro and micromorphological characteristics of seeds of these species for clearer diagnosis at the stage of fruiting is low informative.

Strength and Damping Properties of Cementitious Composites Incorporating Original and Alkali Treated Flax Fibers

This experimental investigation focused on the mechanical and damping properties of raw and alkali treated flax fibers reinforced cementitious composites. The objective of this study was to research the influence of flax fiber for the damping properties of cementitious composites. The mechanical strengths were obtained using compressive and flexural tests and damping properties were measured using a dynamic mechanical analysis. Structural modifications to the treated flax fibers were analyzed using scanning electronic microscopy and mercury intrusion porosimetry. Results showed that cement compressive strength was decreased, and flexural strength was increased by the addition of untreated flax fibers. Alkali treatment improved the mechanical strength of flax reinforced cement. The addition of flax fibers improved cement damping properties, and the damping effect of untreated flax fibers was greater than that of alkali treated fibers. The loss tangent of the cementitious composites reinforced with raw and alkali treated flax fibers at an amount of 1 wt % of cement was improved by 45.83% and 37.5% at 20 Hz, respectively. The damping properties of flax fiber was attributed to friction between elementary fibers and between adjacent fiber cell walls and slipping between the fiber and matrix. Callouses, pectin, and lignocellulose in untreated flax fiber contributed to damping and their removal by alkali treatment was the main reason why untreated flax fibers had a greater damping effect as compared to alkali-modified flax fibers.

Geometric analysis of intrusive growth of wood fibres in Robinia pseudoacacia

ABSTRACT All cell types of the secondary xylem arise from the meristematic cells (initials) of the vascular cambium and grow under mechanical constraints emerging from the circular-symmetrical geometry that characterises many tree trunks. The course of intrusive growth of cambial initials has been elucidated, but is yet to be described in the case of xylem fibres. This study explains the geometry of intrusive growth of the secondary xylem fibres in the trunk of Robinia pseudoacacia. Long series of serial semi-thin sections of the vascular cambium and the differentiating secondary xylem were analysed. Since fibres grow in close vicinity to expanding cells of the derivatives of the vascular cambium, we assumed that they have similar growth conditions. Dealing with the cylindrical tissue of the vascular cambium in a previous study, we used a circularly symmetrical equation for describing the growth mechanism of cambial initials. Like the cambial initials, some of the cambial derivatives differentiating into the various cell types composing the secondary xylem also exhibit intrusive growth between the tangential walls of adjacent cells. As seen in cross sections of the cambium, intrusively growing initials form slanted walls by a gradual transformation of tangential (periclinal) walls into radial (anticlinal) walls. Similarly, the intrusive growth of xylem fibres manifests initially as slants, which are formed due to axial growth of the growing cell tips along the tangential walls of adjacent cells. During this process, the tangential walls of adjacent cells are partly separated and dislocated from the tangential plane. The final shape of xylem fibres, or that of vessel elements and axial parenchyma cells, depends upon the ratio of their intrusive versus symplastic growths in the axial, circumferential and radial directions.

Ontogeny of anastomosed laticifers in the stem apex of Hancornia speciosa (Apocynaceae): a topographic approach.

Latex is a complex plant secretion with both ecological and economic importance. There is little information currently available on the cytological aspects of the ontogenesis of anastomosed laticifers, the ducts originating through the lysis of adjacent cell walls. Hancornia speciosa is a tree typical of the Cerrado (neotropical savanna) biome. Its latex has medicinal value and is also used to produce rubber. The ontogenesis of its laticifers and the process of latex synthesis are described here. Structural, cytochemical, and ultrastructural analyses of the stem apex and phytochemical analyses of the latex were performed. Laticifer ontogenesis begins early in promeristem cells and subsequently extends through the procambial region. The laticifer precursor cells demonstrate intense metabolic activity, evidenced by starch accumulation and the proliferation of mitochondria, dictyosomes, endoplasmic reticulum, and ribosomes-resulting in the thickening of the cell walls and accumulations of oil droplets in the cytoplasm and fibrous materials in the vacuoles. The ontogenetic process culminates with the partial dissolution of adjacent cell walls and the collapse of the cytoplasm, giving rise to anastomosed laticifers distributed throughout the phloem and adjacent regions of the cortex and medulla. The latex itself is composed of terpenes, mucilage, proteins, alkaloids, and organelle residues that form an emulsion. Laticifer development takes place in three phases: (1) the formation of the emulsion in the promeristem, (2) anastomosis and the collapse of the cytoplasm in the distal region of the procambium, and (3) the maturation of laticifers and latex storage in a central vacuole in the proximal region of the procambium.

Molecular phylogeny, systematics, and revision of the type species of Lobomonas, L.francei (Volvocales, Chlorophyta) and closely related taxa.

In the present study, three new strains of the rare volvocalean green alga Lobomonas were isolated from field-collected samples, one from Sardinia (Italy) and two from Argentina, and comparatively studied. The Sardinian and one of the Argentinian strains were identified as Lobomonas francei, the type species of the genus, whereas the second Argentinian strain corresponded to L.panduriformis. Two additional nominal species of Lobomonas from culture collections (L.rostrata and L.sphaerica) were included in the analysis and shown to be morphologically and molecularly identical to the L.francei strains. The presence, number, and shapes of cell wall lobes, the diagnostic criterion of Lobomonas, were shown to be highly variable depending on the chemical composition of the culture medium used. The analyses by SEM gave evidence that the cell wall lobes in Lobomonas originate at the junctions of adjacent cell wall plates by extrusion of gelatinous material. The four L.francei strains had identical nrRNA gene sequences and differed by only one or two substitutions in the ITS1+ITS2 sequences. In the phylogenetic analyses, L.francei and L.panduriformis were sister taxa; however, another nominal Lobomonas species (L.monstruosa) did not belong to this genus. Lobomonas, together with taxa designated as Vitreochlamys, Tetraspora, and Paulschulzia, formed a monophyletic group that in the combined analyses was sister to the "Chlamydomonas/Volvox-clade." Based on these results, Lobomonas was revised, the diagnosis of the type species emended, a lectotype and an epitype designated, and several taxa synonymized with the type species.

About the influence of a water-based priming system on the interactions between wood and one-component polyurethane adhesive studied by atomic force microscopy and confocal Raman spectroscopy imaging

In recent years one-component polyurethane (1C-PUR) adhesives have been increasingly applied in loadbearing timber constructions. However, a low wood fracture percentage at wet stage and a comparatively low performance in delamination tests need to be overcome to fulfill certain technical standards and to achieve an even higher acceptance in the global market of wood adhesives. Via the use of suitable primers, in this case a water-soluble primer (WSP) system, it is possible to significantly improve these parameters. Nevertheless, the exact mode of action of the primer remains so far unclear. In this study, primer treated wood surfaces were characterized in detail by atomic force microscopy (AFM) and the influence of the primer on the penetration of the 1C-PUR adhesive into wood cell walls was further analyzed by confocal Raman spectroscopy imaging. AFM investigations of primer treated surfaces reveal a significant increase in adhesion compared to non-primer treated surfaces and indicate a penetration of the primer into the cell wall. However, Raman spectroscopy shows that the primer application does not lead to a substantial and deep penetration of the 1C-PUR adhesive into wood cell walls. Slight changes in the spectral properties of the primer treated samples at the interface between the bond line and the adjacent cell wall, point towards changed interactions between wood and polyurethane.