Additional Strategies Research Articles

Nutrition counsellors’ recommended eating patterns for individuals with type 2 diabetes in the USA

IntroductionMultiple eating patterns can promote glycaemic control and weight loss among patients with type 2 diabetes mellitus (T2D). Clinical practice guidelines for T2D management encourage health professionals to guide patients’...

Social contact as a strategy to reduce stigma in low- and middle-income countries: A systematic review and expert perspectives.

Social contact (SC) has been identified as a promising strategy for stigma reduction. Different types of SC exist. Various scholars defined positive factors to strengthen SC. This study aims to investigate the application and effectiveness of SC as a strategy to reduce stigmatisation across stigmas, settings and populations in low- and middle-income countries (LMICs). We specifically examine the use of positive factors. A systematic review was conducted in twelve electronic databases using key terms related to stigma AND social contact AND intervention AND LMICs. Data were synthesised narratively. Study quality was assessed with the Joanna Briggs Institute critical appraisal checklists. Additionally, semi-structured interviews were used with first/corresponding authors of included publications to investigate their practical experiences with SC. Forty-four studies (55 publications) were identified. Various stigmas (n = 16) were targeted, including mental health (43%). Indirect (n = 18) and direct contact (n = 16) were used most frequently, followed by collaboration, imagined and vicarious contact, or a combination. The most applied additional strategy was education. Almost half of the studies, explicitly or implicitly, described positive factors for SC, such as PWLE training or disconfirming stereotypes. The majority suggested that SC is effective in reducing stigma, although inconsistent reporting overshadows conclusions. Perspectives of people with lived experience (PWLE) were infrequently included. Expert perspectives stressed the importance of contextualisation, PWLE participation, and evaluation of SC. This study provides an overview of SC as a stigma reduction strategy within LMICs. Conclusions about which type of SC is more effective or whether SC is more effective for a specific stigma category cannot be drawn. We recommend future research to strengthen reporting on effectiveness as well as PWLE perspective and SC processes, and to further critically examine the potential of SC. An overview of positive factors applied to strengthen SC is provided, which can stimulate reflection and guide future SC.

Role of Phytoactive-based Nanoformulation for the Treatment of Arthritis.

Joints and arthritic conditions are among the most dangerous illnesses that humans have ever encountered and it is even more worrying that there is no recognized treatment for arthritis. The researchers looked for safer alternatives, such as herbal medicines, because the traditional treatments used to treat severe joint inflammatory issues have several negative side effects. A ligand-coated nanomedicine can bind to receptors that are overexpressed by cells in chronically inflammatory tissues, increasing its efficacy and reducing its systemic side effects. This is because the pathophysiology of rheumatoid arthritis suggests that macrophages and overexpressed molecules exist within inflamed tissues, which increases permeability and allows nanomedicines to accumulate in inflamed tissue and cause retention phenomena. The anti-arthritic properties of a variety of plants, their components, extracts, and phyto-isolates have been studied to date. These plant compounds can pose stability and delivery problems, which restricts their efficacy as a treatment for inflammatory diseases. The multifunctional and adaptable features of different nanoparticles can help herbal remedies based on nanotechnology get beyond the delivery constraints of different natural ingredients. The application of nanoformulations in tissue engineering is an additional strategy for delivering drugs directly to bone and cartilage in RA patients. The medication is more therapeutically effective due to nanoformulation's improved synovium and cartilage absorption, accumulation, and penetration at inflammatory joints. Herbal medications with a nanotechnology foundation exhibit superior pharmacokinetic and drug delivery qualities, aid in better oral absorption, regulate drug release, boost in vivo retention capacity, target delivery, and have synergistic effects. This review provides an update on the use of herbal medicines based on nanotechnology, which show promise in treating arthritis and other ailments.

VOC source apportionment, reactivity, secondary transformations, and their prioritization using fuzzy-AHP method in a coal-mining city in India.

This study assessed the air quality status in different functional zones of Dhanbad-a coal-mining and industrial hub, based on the measurement of aromatic and halogenated volatile organic compounds (VOCs) using gas chromatography. The study encompasses source apportionment of VOCs and their chemical reactivity in terms of OH radical loss rate (LOH), ozone-forming potential (OFP), and their secondary organic aerosol forming potential (SOAp). Furthermore, prioritization of VOCs based on a fuzzy-analytical hierarchical process (F-AHP) has also been done. The results found xylene species to have the highest concentration in all three seasons across traffic-intersection and industrial zones and toluene at the institutional zone. The study identified four sources using positive matrix factorization (PMF) model, viz., mixed traffic exhaust (35%), coal combustion sources (30%), industrial (26%), and solvent usage (9%). LOH and SOAp were ~ 16 times more at the industrial and traffic-intersection zone than the institutional zone. The aromatic species contributed 97% to the OFP, and many species exhibited less contribution to the mixing ratio of VOCs but displayed a high contribution to LOH, OFP, and SOAp, suggesting the need to prefer reactivity-based strategies in addition to concentration-based strategies in the future for their regulation. The F-AHP-based priority component analysis identified 16 species out of 29 in the priority watch list (nine in tier-1, four in tier-2, and three in tier-3). The paucity of data and lack of ambient air quality standards on VOCs (except benzene) make it difficult to determine which aspect should be dealt with first and which species require more attention. Therefore, the F-AHP method used in this study could help identify the influencing parameters to be considered while devising efficient VOC management policies.

The New Cyberlinktech, Inc. Resilience to Creating Growth Model

The New Cyberlinktech, Inc. (TNCI) operates in a highly dynamic and evolving market, requiring the need for a strategic business plan to sustain growth and adaptability. The New Cyberlinktech, Inc. (TNCI) is one of the leading information technology providers for Private Emission Testing Centers (PETCs) under the Department of Transportation and the Land Transportation Office in the Philippines. TNCI faces significant challenges in the current market landscape, including increasing regulatory pressure against PETCs, the emergence of the Motor Vehicle Inspection System (MVIS) as an alternative mode of vehicle testing, and the frequent leadership changes and abrupt policy shifts at the Land Transportation Office (LTO) and the Department of Transportation (DOTr). To ensure sustainability and adaptability, this dissertation aims to develop a comprehensive business plan for The New Cyberlinktech, Inc. (TNCI) that addresses the challenges posed by the rapidly changing market landscape including a 5-year roadmap. The research design employs a mixed methods approach. The qualitative phase involves conducting interviews with key stakeholders within TNCI, including senior management, employees, and selected industry experts. Through these qualitative interactions, the study aims to identify the specific challenges faced by TNCI in the evolving market and explore potential strategic approaches to address them. In the quantitative phase, the focus is on using surveys among the current clients of the company to determine the preferred IT projects that they want the company to implement in the future. The quantitative analysis provides a comprehensive assessment of TNCI’s current performance, identifies potential risks and opportunities, and informs the development of strategic recommendations. The integrated analysis phase synthesizes the findings from the qualitative and quantitative data. By comparing and contrasting the insights gained from both approaches, a holistic understanding of the challenges and opportunities facing TNCI is achieved. The strategic business plan is formulated based on the integrated analysis, considering the specific needs and goals of TNCI. The plan encompasses strategies to address regulatory challenges, navigate the MVIS landscape, manage leadership changes, adapt to policy shifts, and recommend potential IT projects that the company can adapt and implement. Potential findings may include insights into the specific regulatory pressures faced by PETCs, the implications of the MVIS on TNCI’s business model, and strategies to mitigate the effects of leadership and policy changes. The implications of the research findings can inform TNCI’s decision-making process and help guide the company in sustaining growth and adaptability in the rapidly evolving market. This dissertation contributes to the body of knowledge by providing a comprehensive strategic business plan tailored to TNCI’s context. The findings and recommendations have practical implications for TNCI and other IT companies operating in similar environments. Future research opportunities may involve evaluating the implementation and effectiveness of the proposed business plan and exploring additional strategies to enhance TNCI’s competitive advantage in the PETC industry. Keywords: Strategic continuity, Business plan, Sustainability, Adaptability, Private Emission Testing Centers, Motor Vehicle Inspection System, Regulatory pressure, Leadership change, Policy shifts, mixed methods researc

Pediatric Cancer Drug Development: Leveraging Insights in Cancer Biology and the Evolving Regulatory Landscape to Address Challenges and Guide Further Progress.

The discovery and development of anticancer drugs for pediatric patients have historically languished when compared to both past and recent activity in drug development for adult patients, notably the dramatic spike of targeted and immune-oncology therapies. The reasons for this difference are multifactorial. Recent changes in the regulatory landscape surrounding pediatric cancer drug development and the understanding that some pediatric cancers are driven by genetic perturbations that also drive disparate adult cancers afford new opportunities. The unique cancer-initiating events and dependencies of many pediatric cancers, however, require additional pediatric-specific strategies. Research efforts to unravel the underlying biology of pediatric cancers, innovative clinical trial designs, model-informed drug development, extrapolation from adult data, addressing the unique considerations in pediatric patients, and use of pediatric appropriate formulations, should all be considered for efficient development and dosage optimization of anticancer drugs for pediatric patients.

Improving detection of oral lesions: Eye tracking insights from a randomized controlled trial comparing standardized to conventional approach.

Early detection of oral cancer (OC) or its precursors is the most effective measure to improve outcome. The reasons for missing them on conventional oral examination (COE) or possible countermeasures are still unclear. In this randomized controlled trial, we investigated the effects of standardized oral examination (SOE) compared to COE. 49 dentists, specialists, and dental students wearing an eye tracker had to detect 10 simulated oral lesions drawn into a volunteer's oral cavity. SOE had a higher detection rate at 85.4% sensitivity compared to 78.8% in the control (p = 0.017) due to higher completeness (p < 0.001). Detection rate correlated with examination duration (p = 0.002). A standardized approach can improve systematics and thereby detection rates in oral examinations. It should take at least 5 min. Perceptual and cognitive errors and improper technique cause oral lesions to be missed. Its wide implementation could be an additional strategy to enhance early detection of OC.

Green Exercise, Blue Spaces and Active Leisure Events: The Performance of New Participants is Associated With Their Response to Event Characteristics

Active leisure events (ALEs) promoting activity among non-traditional sporting participants are an increasingly important part of health interventions and social prescribing. Identifying characteristics of ALEs that encourage engagement are key for enhancing their efficacy. Models revealed first-time participants returned to parkrun more quickly if they were male, older, performed poorly, attended a larger event with more new adult participants, a hard surface type and with woodland and freshwater on its route. Interaction terms between performance and event characteristics revealed poor performing new participants were particularly influenced by event size and less influenced by woodland and freshwater suggesting that they might find it easier to hide at large events and feel less out of place. This highlights the importance not just of identifying characteristics of ALEs that influence return rates but also identifying interaction terms with performance so the behavior of target demographics can be better understood. Organisers of ALEs might want to consider prioritizing the use of routes that maximize exposure to woodland and freshwater and consider introducing additional strategies designed to make less fit participants feel that they belong.

Evaluating Wall-Mounted Prompts to Facilitate Physical Activity-Related Discussion between Individuals with Cancer and Oncology Health Care Providers: A Pre-post Survey Study.

To evaluate the use of wall-mounted prompts in facilitating physical activity (PA)-related discussions between individuals with cancer and oncology care providers. Individuals with cancer were approached to participate in a survey-based pre-post study. Half of participants (n = 100) completed a survey prior to installation of wall-mounted prompts in clinic while the other half (n = 100) completed a survey following installation of the prompts. Survey questions included content of PA-related discussion, satisfaction with PA education across treatment, and current PA level. The post-prompt survey also asked questions related to the prompt. Survey responses were analyzed using descriptive statistics. Chi-squared tests were performed to determine significance between timepoints. One hundred participants completed the survey at each timepoint. A significant difference was found pre and post-prompt in the number of PA discussions occurring overall during care (p = 0.03). Some participants (53%) were satisfied with the PA education received during treatment. There was no significant difference in occurrence of PA discussion (p = 0.36) pre and post-prompt and no difference in PA behaviour was observed (p = 0.130). Wall-mounted prompts may be effective in increasing the frequency of PA-related discussions between individuals with cancer and their oncology team across treatment. Additional strategies, such as easy referral to rehabilitation professionals, are also needed to facilitate safe and effective PA behaviour during and after cancer treatments.

A Novel Decentralized Frequency Regulation Method of Renewable Energy Stations Based on Minimum Reserve Capacity for Renewable Energy-Dominated Power Systems

In this paper, we propose a novel decentralized frequency regulation method for renewable energy-dominated power systems. First, the system is modularized into unified frequency regulation modules composed of synchronous generators(SGs) and renewable energy stations. Then, based on the aggregation model of the module, a renewable energy station frequency regulation method based on robust control barrier functions(RCBFs) is proposed to ensure that the frequency deviation is limited, and an additional switching control strategy is complemented to avoid the long-term suspension of over-high quasi-steady-state frequency. The proposed method is still applicable under the coordination of multiple renewable energy stations. Each renewable energy station deploys decentralized control based on local information, which can support fast frequency response. The robustness of frequency response time constants of renewable energy stations and frequency dead zone are considered, which makes the control method easy to implement. A module transient frequency stability margin index based on reserve capacity is proposed, which can be used to guide the system planning and operation. Finally, simulation results validate the applicability of the proposed method, demonstrating its efficacy in regulating frequency in renewable energy-dominated power systems.

The effect of the four pharmacological pillars of heart failure on haemoglobin level.

Anaemia, a condition characterized by low levels of haemoglobin, is frequently observed in patients with heart failure (HF). Guideline-directed medical therapy improves HF outcomes by using medications like beta blockers, angiotensin-converting enzyme inhibitors, and angiotensin receptor blockers, along with mineralocorticoid receptor antagonists and sodium-glucose cotransporter 2 inhibitors. In this study, we aimed to review the pathophysiology of anaemia in patients with HF and present the current evidence regarding the relationship between the main recommended medications for these patients and haemoglobin levels. The authors conducted a comprehensive search in the medical literature for relevant original clinical articles in which the four pharmacological pillars of HF were given to the patients; we, then, assessed whether the association of use of these medications and haemoglobin level or development of anaemia was provided. These common medications have been shown in the literature that may exacerbate or ameliorate anaemia. Besides, it has been shown that even in the case that they result in the development of anaemia, their use is associated with positive effects that outweigh this potential harm. The literature also suggests that among patients receiving medications with negative effects on the level of haemoglobin, there was no difference in the rate of mortality between anaemic and non-anaemic patients when both were on treatment for anaemia; this point highlights the importance of the detection and treatment of anaemia in these patients. Further research is needed to explore these relationships and identify additional strategies to mitigate the risk of anaemia in this population.

PAM-Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Applications.

The RNA-programmed CRISPR effector protein Cas12a has emerged as a powerful tool for gene editing and molecular diagnostics. However, additional bio-engineering strategies are required to achieve control over Cas12a activity. Here, we show that Toehold Switch DNA hairpins, presenting a rationally designed locked protospacer adjacent motif (PAM) in the loop, can be used to control Cas12a in response to molecular inputs. Reconfiguring the Toehold Switch DNA from a hairpin to a duplex conformation through a strand displacement reaction provides an effective means to modulate the accessibility of the PAM, thereby controlling the binding and cleavage activities of Cas12a. Through this approach, we showcase the potential to trigger downstream Cas12a activity by leveraging proximity-based strand displacement reactions in response to target binding. By utilizing the trans-cleavage activity of Cas12a as a signal transduction method, we demonstrate the versatility of our approach for sensing applications. Our system enables rapid, one-pot detection of IgG antibodies and small molecules with high sensitivity and specificity even within complex matrices. Besides the bioanalytical applications, the switchable PAM-engineered Toehold Switches serve as programmable tools capable of regulating Cas12a-based targeting and DNA processing in response to molecular inputs and hold promise for a wide array of biotechnological applications.

Personal Recovery Among People at Risk for Developing Serious Mental Health Problems: A Qualitative Systematic Review.

Personal recovery refers to a person's pursuit of a full, meaningful life despite the potentially debilitating impact of a mental illness. An evidence base describing personal recovery among people at risk for developing a mental illness is lacking, limiting the potential for mental health services to support personal recovery. To address this gap, the authors synthesized the extant research describing personal recovery among people at risk for developing a mental illness. A systematic search of several literature databases (MEDLINE, Embase, APA PsycInfo, Web of Science Core Collection, and Cochrane Library) was conducted to retrieve qualitative and case studies and first-person accounts. The Joanna Briggs Institute guidelines for systematic reviews were followed. Included studies reported on participants at variable risk for developing a schizophrenia spectrum, bipolar, major depressive, or borderline personality disorder. Articles were retrieved through a librarian-assisted search and through use of additional strategies (e.g., expert consultation). Abstracts were screened by the research team, and themes were developed by using thematic synthesis. The 36 included articles were synthesized, and six themes were generated: difficulties and challenges; establishing an understanding of, and finding ways to cope with, one's mental health challenges; reestablishing a sense of agency and personhood; receiving support from people and services, as well as restoring relationships; reestablishing hope, meaning, and purpose; and overcoming stigma and destigmatizing mental illness in others. These findings provide a conceptual foundation that can guide future research on personal recovery and clinical interventions that foster it among people at risk for mental illness.

Гибридный дискурс: конституирующие признаки географических очерков

The research objective is to identify the constitutive features of a geographic essay as a distinct type of discourse. The scientific novelty lies in conducting for the first time the study of the hybrid nature of the 19th to early 20th-century geographic essays, determining and analyzing its primary components. The article also introduces the author's definition of the term “hybrid discourse”. The research is based on the classification proposed by V. I. Karasik, who suggested considering discourse participants, goals, values, chronotopes, themes, genres, strategies, precedent texts, and discursive formulas as the main components of discourse. The study revealed that scientific and artistic discourses form the discursive basis of geographic essays. The key constitutive features of the 19th to early 20th-century geographic essays are presented and described in the analysis. The research identified the key elements of the scientific discourse including main objectives, values, themes, primary chronotopes, central informational strategy, precedent texts, and discursive formulas. The artistic component of these essays is expressed through the formation of secondary objectives, as well as additional chronotope frameworks and diverse frequency strategies.

Compassion Meditation for Distressed Older Veterans: A Feasibility Study

ABSTRACT Objectives Older Veterans are at elevated risk for psychological distress and may encounter barriers to accessing mental health services. Compassion Meditation (CM) promotes positive emotions and outcomes among distressed individuals; thus, we conducted a preliminary feasibility study of CM among distressed older Veterans. Methods Participants included 25 Veterans aged 55+ (M = 69.0, SD = 10.6) with anxiety and/or depressive symptoms, recruited from primary care, mostly male (76.0%), and White (60.0%). CM consisted of 10 groups, which were transitioned from in-person to telehealth due to COVID-19. Feasibility indices included rates of intervention initiation and completion, and attendance. Participants completed measures of symptom severity and well-being pre- and post-intervention. Results Of 25 enrolled participants, 88.0% (n = 22) attended at least one session, and 52% (n = 13) completed the intervention (attended six or more sessions). Among intervention completers, the average number of sessions attended was 9.46. Seven Veterans withdrew from intervention due to difficulties engaging via telehealth. Conclusions These findings support the feasibility of CM training in older Veterans with psychological distress, though dropouts highlighted potential need for additional strategies to facilitate telehealth participation. Clinical Implications Older Veterans appear amenable to meditation-based practices, provided they are easy to access.

Lovastatin/SN38 co-loaded liposomes amplified ICB therapeutic effect via remodeling the immunologically-cold colon tumor and synergized stimulation of cGAS-STING pathway

Current immune checkpoint blockade (ICB) immunotherapeutics have revolutionized cancer treatment. However, many cancers especially the “immunologically cold” tumors, do not respond to ICB, prompting the search for additional strategies to achieve durable responses. The cGAS-STING pathway, as an essential immune response pathway, has been demonstrated for a potent target to sensitize ICB immunotherapy. However, the low efficiency of conventional STING agonists limits their clinical application. Recent studies have shown that DNA topoisomerase I (TOPI) inhibitor chemodrug SN38 can activate the cGAS-STING pathway and induce an immune response through DNA damage, while the traditional statins medication lovastatin was found to inhibit DNA damage repair, which may in turn upregulate the damaged DNA level. Herein, we have developed a liposomal carrier co-loaded with SN38 and lovastatin (SL@Lip), which can be accumulated in tumors and efficiently released SN38 and lovastatin, addressing the problem of weak solubility of these two drugs. Importantly, lovastatin can increase DNA damage and enhance the activation of cGAS-STING pathway, coordinating with SN38 chemotherapy and exhibiting the enhanced combinational immunotherapy of PD-1 antibody by remodeling the tumor microenvironment in mouse colorectal cancer of both subcutaneous and orthotopic xenograft models. Overall, this study demonstrates that lovastatin-assisted cGAS-STING stimulation mediated by liposomal delivery system significantly strengthened both chemotherapy and immunotherapy of colorectal cancer, providing a clinically translational strategy for combinational ICB therapy in the “immunologically cold” tumors.

Strengthening of high nitrogen austenitic stainless steel by Nb addition

Nitrogen addition is known to be one of the most effective ways of strengthening austenitic stainless steels while maintaining good properties of the steels. This study investigates Nb addition as an additional strategy to strengthen a high nitrogen austenitic stainless steel. It was found that Nb-containing steels developed Z-phase precipitates during homogenization and hot-rolling. Their fraction and size hardly changed by subsequent aging at 1050 °C. They effectively delayed grain coarsening and fine grains were maintained throughout the aging. The yield strengths of Nb-containing steels were always higher than those of Nb-free steel by 30–83 MPa. Quantitative analyses revealed that the major strengthening mechanism was grain boundary strengthening. The contribution from precipitation hardening was small because the precipitates were not sufficiently fine and the negative contribution from solid solution strengthening due to N consumption by the precipitation was also insignificant.

Pressure-Induced Amidine Formation via Side-Chain Polymerization in a Charge-Transfer Cocrystal.

Compression of small molecules can induce solid-state reactions that are difficult or impossible under conventional, solution-phase conditions. Of particular interest is the topochemical-like reaction of arenes to produce polymeric nanomaterials. However, high reaction onset pressures and poor selectivity remain significant challenges. Herein, the incorporation of electron-withdrawing and -donating groups into π-stacked arenes is proposed as a strategy to reduce reaction barriers to cycloaddition and onset pressures. Nevertheless, competing side-chain reactions between functional groups represent alternative viable pathways. For the case of a diaminobenzene:tetracyanobenzene cocrystal, amidine formation between amine and cyano groups occurs prior to cycloaddition with an onset pressure near 9 GPa, as determined using vibrational spectroscopy, X-ray diffraction, and first-principles calculations. This work demonstrates that reduced-barrier cycloaddition reactions are theoretically possible via strategic functionalization; however, the incorporation of pendant groups may enable alternative reaction pathways. Controlled reactions between pendant groups represent an additional strategy for producing unique polymeric nanomaterials.

Impact of U.S. Federal Loan Repayment Programs on the Behavioral Health Workforce: Scoping Review.

Federal loan repayment programs (LRPs) are one strategy to address the shortage of behavioral health providers. This scoping review aimed to identify and characterize the federal LRPs' impact on the U.S. behavioral health workforce. A scoping review was conducted in accordance with JBI (formerly known as the Joanna Briggs Institute) methodology for scoping reviews. The authors searched the Ovid MEDLINE, Web of Science, APA PsycInfo, EconLit, PAIS Index, and Embase databases, and gray literature was also reviewed. Two coders screened each article's abstract and full text and extracted study data. Findings were narratively synthesized and conceptually organized. The full-text screening identified 17 articles that met eligibility criteria. Of these, eight were peer-reviewed studies, and all but one evaluated the National Health Service Corps (NHSC) LRP. Findings were conceptually organized into five categories: descriptive studies of NHSC behavioral health needs and the NHSC workforce (k=4); providers' perceptions of, and experiences with, the NHSC (k=2); associations between NHSC funding and the number of NHSC behavioral health providers (k=4); NHSC behavioral health workforce productivity and capacity (k=3); and federal LRP recruitment and retention (k=4). The literature on federal LRPs and their impact on the behavioral health workforce is relatively limited. Although federal LRPs are an important and effective tool to address the behavioral health workforce shortage, additional federal policy strategies are needed to attract and retain behavioral health providers and to diversify the behavioral health workforce.

Targeting LEF1-mediated epithelial-mesenchymal transition reverses lenvatinib resistance in hepatocellular carcinoma.

Acquired resistance is a significant hindrance to clinical application of lenvatinib in unresectable hepatocellular carcinoma (HCC). Further in-depth investigation of resistance mechanisms can help to develop additional therapeutic strategies to overcome or delay resistance. In our study, two lenvatinib-resistant (LR) HCC cell lines were established by treatment with gradient increasing concentration of lenvatinib, named Hep3B-LR and HepG2-LR. Interestingly, continuous lenvatinib treatment reinforced epithelial-mesenchymal transition (EMT), cell migration, and cell invasion. Gene set enrichment analysis (GSEA) enrichment analysis of RNA-sequencing from Hep3B-LR and corresponding parental cells revealed that activation of Wnt signaling pathway was involved in this adaptive process. Active β-catenin and its downstream target lymphoid enhancer binding factor 1 (LEF1) were significantly elevated in LR HCC cells, which promoted lenvatinib resistance through mediating EMT-related genes. Data analysis based on Gene Expression Omnibus (GEO) and the Cancer Genome Atlas Program (TCGA) databases suggests that LEF1, as a key regulator of EMT, was a novel molecular target linked to lenvatinib resistance and poor prognosis in HCC. Using a small-molecule specific inhibitor ICG001 and knocking down LEF1 showed that targeting LEF1 restored the sensitivity of LR HCC cells to lenvatinib. Our results uncover upregulation of LEF1 confers lenvatinib resistance by facilitating EMT, cell migration, and invasion of LR HCC cells, indicating that LEF1 is a novel therapeutic target for overcoming acquired lenvatinib resistance.