To evaluate the impact of the phloem flux on the pesticide uptake process in potatoes, this study developed a phloem-adjusted model based on the classic model that focuses mainly on the diffusion process. To achieve high-throughput simulations, we introduced an approximate method to convert the phloem flux transport process into a simple specific uptake rate of pesticides. In comparison to the classic model (non-phloem model), the phloem-adjusted model generated higher pesticide concentrations and bioconcentration factors (BCFs) in potatoes, owing to the additional pesticide uptake route introduced to the adjusted model. However, the simulation, which was conducted for 740 pesticides, indicated that for most pesticides, the phloem flux route did not contribute a significant portion of the pesticide uptake to potato tubers compared with the soil diffusion route. This was further characterized, using the differential factor (DF), to evaluate the difference in the simulated results between the proposed model and classic models. The largest DF (~0.11) was obtained for pesticides with moderate lipophilicity (i.e., log KOW of 3.0), indicating that only a difference of 10% was generated between the two models. The 10% increase in pesticide concentration (or BCFs) in potatoes, simulated by the phloem-adjusted model, was within the acceptable uncertainty interval of the classic model, thus confirming the validity of using the classic model to predict the pesticide uptake process in potato tubers. However, we found that the negligibility of the phloem flux route was not merely due to hydrophobicity (i.e., hypothesis of the classic model), but was related to the i) plant physiology of potatoes, ii) lipophilicity of a pesticide, and iii) the diffusivity of a pesticide in water. Although future studies on pesticide concentrations in phloem sap and the dynamic growth of potatoes need to be undertaken, the model developed in this study reveals a more comprehensive pesticide uptake process in potatoes, which can promote the understanding of the pesticide uptake mechanism in potatoes.