Addition Of Granular Activated Carbon Research Articles

Enhancing anammox process with granular activated carbon: A study on Microbial Extracellular Secretions (MESs)

Granular activated carbon (GAC), a porous carbon-based material, provides increased attachment space for functional microorganisms and enhances nitrogen removal by facilitating extracellular electron transfer in the anammox process. This study investigates the effects of GAC on the biosynthesis of microbial extracellular secretions (MESs) and explores the roles of these secretions in anammox activities. Four lab-scale reactors were operated: two downstream UASB reactors (D1 and D2) receiving effluents from the upstream UASB reactors (U1: no-GAC, U2: yes-GAC). Our results indicate that MESs were enhanced with the addition of GAC. The effluent from U2 exhibited a 59.62 % higher amino acid content than that from U1. These secretions contributed to an increase in the nitrogen loading rate (NLR) in the downstream reactors. Specifically, NLR in D1 increased from 130.5 to 142.7 g N/m3/day, and in D2, it escalated from 137.5 to 202.8 g N/m3/day, likely through acting as cross-feeding substrates or vital nutrients. D2 also showed increased anammox bacterial activity, enriched Ca. Brocadia population and hao gene abundance. Furthermore, this study revealed that D2 sludge has significantly higher extracellular polymeric substances (EPS) (48.71 mg/g VSS) and a larger average granule size (1.201 ± 0.119 mm) compared to D1 sludge. Overall, GAC-stimulated MESs may have contributed to the enhanced performance of the anammox process.

Enhanced sludge solubilization by a fluidized electrode: Granular activated carbon promoted electrochemical oxidation

Electrochemical sludge pretreatment is receiving increasing attention because of its small footprint and higher environmental compatibility. However, the limited effective area of electrode plates and the low conductivity of sludge hinder the widespread application of electrochemical pretreatment. In this study, granular activated carbon (GAC) was employed to construct a fluidized electrode electrochemical system (FEE) to promote electrochemical pretreatment. Under the optimal operating parameters, the FEE system could effectively facilitate sludge decomposition, indicated by 126% increase in soluble chemical oxygen demand (SCOD) and 23.1% reduction in sludge volume. Mechanism study revealed that the addition of GAC significantly enhanced the conductivity of sludge, thereby promoting the oxidation capacity of FEE system. Furthermore, continuously generated H2O2 in FEE further promoted sludge solubilization. GAC offered an effectively, green and sustainable enhancement approach for sludge electrochemical pretreatment.

Granular activated carbon enhances volatile fatty acid production in the anaerobic fermentation of garden wastes.

Garden waste, one type of lignocellulosic biomass, holds significant potential for the production of volatile fatty acids (VFAs) through anaerobic fermentation. However, the hydrolysis efficiency of garden waste is limited by the inherent recalcitrance, which further influences VFA production. Granular activated carbon (GAC) could promote hydrolysis and acidogenesis efficiency during anaerobic fermentation. This study developed a strategy to use GAC to enhance the anaerobic fermentation of garden waste without any complex pretreatments and extra enzymes. The results showed that GAC addition could improve VFA production, especially acetate, and reach the maximum total VFA yield of 191.55mg/g VSadded, which increased by 27.35% compared to the control group. The highest VFA/sCOD value of 70.01% was attained in the GAC-amended group, whereas the control group only reached 49.35%, indicating a better hydrolysis and acidogenesis capacity attributed to the addition of GAC. Microbial community results revealed that GAC addition promoted the enrichment of Caproiciproducens and Clostridium, which are crucial for anaerobic VFA production. In addition, only the GAC-amended group showed the presence of Sphaerochaeta and Oscillibacter genera, which are associated with electron transfer processes. Metagenomics analysis indicated that GAC addition improved the abundance of glycoside hydrolases (GHs) and key functional enzymes related to hydrolysis and acidogenesis. Furthermore, the assessment of major genera influencing functional genes in both groups indicated that Sphaerochaeta, Clostridium, and Caproicibacter were the primary contributors to upregulated genes. These findings underscored the significance of employing GAC to enhance the anaerobic fermentation of garden waste, offering a promising approach for sustainable biomass conversion and VFA production.

Granular activated carbon enhances the anaerobic digestion of solid and liquid fractions of swine effluent at different mesophilic temperatures

ObjectivesThis study evaluated the effect of particle size and dosage of granular activated carbon (GAC) on methane production from the anaerobic digestion of raw effluent (RE) of swine wastewater, and the solid (SF) and liquid (LF) fractions. The effect of temperature using the selected size and dosage of GAC was also evaluated. Methods60 mL of swine wastewater were inoculated with anaerobic granular sludge and GAC at different dosages and particle size. The cultures were incubated at different temperatures at 130 rpm. The kinetic parameters from experimental data were obtained using the Gompertz model. ResultsThe cultures with the LF and GAC (75–150 μm, 15 g/L) increased 1.87-fold the methane production compared to the control without GAC. The GAC at 75–150 μm showed lower lag phases and higher Rmax than the cultures with GAC at 590–600 μm. The cumulative methane production at 45 °C with the RE + GAC was 7.4-fold higher than the control. Moreover, methane production at 45 °C significantly increased with the cultures LF + GAC (6.0-fold) and SF + GAC (2.0-fold). The highest production of volatile fatty acids and ammonium was obtained at 45 °C regardless of the substrate and the addition of GAC contributed to a higher extent than the cultures lacking GAC. In most cases, the kinetic parameters at 30 °C and 37 °C were also higher with GAC. ConclusionsGAC contributed to improving the fermentative and methanogenesis stages during the anaerobic digestion of fractions, evidenced by an improvement in the kinetic parameters.

Pilot-scale aerobic granular sludge reactors with granular activated carbon for effective nitrogen and phosphorus removal from domestic wastewater

Aerobic granular sludge (AGS) is a breakthrough biotechnology of 21st century and an innovative alternative to activated sludge for treating wastewater. Concerns on long-start up periods for development of AGS and stability of granules are impeding its widespread implementation for treating low-strength domestic wastewater especially in tropical climate conditions. Addition of nucleating agents have been shown to improve development of AGS while treating low-strength wastewaters. There are no previous studies on AGS development and biological nutrient removal (BNR) in the presence of nucleating agents during treatment of real domestic wastewater. This study investigated AGS formation and BNR pathways while treating real domestic wastewater in a 2 m3 pilot-scale granular sequencing batch reactor (gSBR) operated without and with granular activated carbon (GAC) particles. The gSBRs were operated under tropical climate (T ≈ 30 °C) for >4-years to evaluate the effect of GAC addition on granulation, granular stability and BNR at pilot-scale. Formation of granules was observed within 3 months. MLSS values of 4 and 8 g/L were recorded within 6 months in gSBRs without and with GAC particles, respectively. The granules had an average size of 1.2 mm and SVI5 of 22 mL/g. Ammonium was mainly removed through nitrate formation in the gSBR without GAC. But, ammonium was removed by short-cut nitrification via nitrite due to washout of nitrite oxidizing bacteria in the presence of GAC. Phosphorus removal was much higher in gSBR with GAC due to the establishment of enhanced biological phosphorus removal (EBPR) pathway. After 3 months, the phosphorus removal efficiencies were at 15 % and 75 %, respectively, without and with GAC particles. The addition of GAC led to moderation in bacterial community and enrichment of polyphosphate-accumulating organisms. This is the first ever report on pilot-scale demonstration of AGS technology in the Indian sub-continent and GAC addition on BNR pathways.

Iron/carbon conductive materials for enhancing anaerobic treatment of synthetic acrylamide wastewater

AbstractBACKGROUNDMost studies have focused on the degradation of acrylamide (AM) by a single strain. There are relatively few studies on the treatment of AM by anaerobic activated sludge. Previous studies have shown that conductive particles can change the mode of electron transport between anaerobic microorganisms. Therefore, this study compared the enhancement effect of three different particles (Fe3O4, granular activated carbon (GAC), graphite) on the treatment of synthetic AM wastewater by anaerobic activated sludge under different temperature conditions (30, 25, 20 °C) and particle dose (2, 6 g L−1).RESULTSThe results showed that 6 g L−1 GAC improved the removal rate of chemical oxygen demand (COD) and AM by 22.95% and 19% compared with the control group at 30 °C, respectively, indicating that the addition of GAC contributes to the degradation of AM and improves COD removal rate. Results of high‐performance liquid chromatography (HPLC) showed that the activated sludge with GAC obviously promoted the degradation of AM. Besides, a large amount of propionic acid was detected in all experimental groups during the reaction, and the activated sludge with GAC promoted degradation of propionic acid.CONCLUSIONAnaerobic activated sludge supplemented with sufficient GAC can not only absorb and degrade AM synchronously but also accelerate the conversion of intermediates, such as acrylic and propionic acids. Based on the results of HPLC and liquid chromatography–mass spectrometry, a possible method of degradation of AM by anaerobic activated sludge was proposed. © 2023 Society of Chemical Industry (SCI).

Boosting resilience of microbial electrolysis cell-assisted anaerobic digestion of blackwater with granular activated carbon amendment

Poor hydrolysis and methanogenesis efficiencies remain the main challenges for blackwater anaerobic digestion. This study investigated the performance of a granular activated carbon (GAC) amended microbial electrolysis cell-assisted anaerobic digester (MEC-AD) treating blackwater. Due to hydrolysis limitation, both MEC-AD and control reactors experienced performance declines as the organic loading rate increased from 3.0 to 4.5 g COD/L-d. Then, adding GAC without mixing formed GAC-sludge aggregates that improved methane yield to 38.3% and 32.3% in the MEC-AD and control reactor, respectively, and enhanced hydrolysis efficiency. The amended MEC-AD also successfully overcame the performance deterioration due to a temperature drop. Biomarker identification revealed the crucial roles of GAC biofilms and settled sludge in promoting methanogenesis and hydrolysis, respectively. This study demonstrated the GAC addition and the electrochemical environment could have a reciprocal influence, leading to more robust syntrophic microbial interactions, which could guide the future application of conductive materials in MEC-AD systems.

Granular activated carbon remediates antibiotic resistance propagation and methanogenic inhibition induced by polystyrene nanoplastics in sludge anaerobic digestion

Nano/microplastics (NPs/MPs) in sewage sludge can induce oxidative stress to the anaerobic digestion (AD) and also proliferate antibiotic resistance genes (ARGs). Recently, granular activated carbon (GAC) has been used as an additive to enhance methane production in AD via direct interspecies electron transfer (DIET); however, its impact on AD exposed to NPs/MPs is yet to be studied. This study examined the effect of GAC (5 and 15 g/L) on sludge AD exposed to 150 µg/L of polystyrene nanoplastics (PsNPs). PsNPs decreased methane yield by 32.3% due to elevated levels of reactive oxygen species. However, GAC addition counteracted this adverse effect and improved methane production, attributed to the potential enrichment of DIET-active microbes and the adsorption of PsNPs by GAC. Moreover, GAC reduced the total abundance of ARGs, which was increased by PsNPs exposure. Thus, GAC can provide dual benefits in mitigating methanogenic inhibition caused by PsNPs and ARG spread.

Granular activated carbon stimulates biogas production in pilot-scale anaerobic digester treating agro-industrial wastewater

This work examines the continuous addition (5 g/L) of conductive granular activated carbon (GAC) in an integrated pilot-scale unit containing an anaerobic digester (180 L) and an aerobic submerged membrane bioreactor (1600 L) connected in series for the treatment of agro-industrial wastewater. Biogas production increased by 32 % after the addition of GAC. Methanosaeta was the dominant methanogen in the digester, and its relative abundance increased after the addition of GAC. The final effluent after post-treatment with the aerobic membrane bioreactor had a total solids content <0.01 g/L and a chemical oxygen demand between 120 and 150 mg/L. A simple cost analysis showed that GAC addition is potentially profitable, but alternatives ways of retaining the GAC in the system need to be found. Overall, this study provides useful scientific data for the possible application of GAC in full-scale biogas projects.

Deciphering the role of granular activated carbon (GAC) in anammox: Effects on microbial succession and communication

Anaerobic ammonium oxidation (anammox) offered an energy-efficient option for nitrogen removal from wastewater. Granular activated carbon (GAC) addition has been reported that improved biomass immobilization, but the role of GAC in anammox reactors has not been sufficiently revealed. In this study, it was observed that GAC addition in an upflow anaerobic sludge blanket (UASB) reactor led to the significantly shortened anammox enrichment time (shortened by 45 days) than the reactor without GAC addition. The nitrogen removal rate was 0.83 kg N/m3/day versus 0.76 kg N/m3/day in GAC and non-GAC reactors, respectively after 255 days’ operation. Acyl-homoserine lactone (AHL) quorum sensing signal molecule C8-HSL had comparable concentrations in both anammox reactors, whereas the signal molecule C12-HSL was more pervasive in the reactor containing GAC than the reactor without GAC. Microbial analysis revealed distinct anammox development in both reactors, with Candidatus Brocadia predominant in the reactor that did not contain GAC, and Candidatus Kuenenia predominant in the reactor that contained GAC. Denitrification bacteria likely supported anammox metabolism in both reactors. The analyses of microbial functions suggested that AHL-dependent quorum sensing was enhanced with the addition of GAC, and that GAC possibly augmented the extracellular electron transfer (EET)-dependent anammox reaction.

Performance Enhancement of an Upflow Anaerobic Dynamic Membrane Bioreactor via Granular Activated Carbon Addition for Domestic Wastewater Treatment

Developing low-carbon advanced processes for sustainable wastewater treatment is of great importance to increase bioenergy recovery and to reduce the greenhouse gas effect. In this study, the influence of adding 25 g/L of granular activated carbon (GAC) on the process performance was studied with a lab-scale GAC amended anaerobic dynamic membrane (G-AnDMBR) used to treat real domestic wastewater, which was compared to a control bioreactor without the GAC addition (C-AnDMBR). Due to the initial adsorption effect of GAC and the high microbial activity of the attached biomass of GAC, the G-AnDMBR achieved a better removal of the total chemical oxygen demand (TCOD) and turbidity compared to the C-AnDMBR, with the average removal rate increasing from 82.1% to 86.7% and from 88.7% to 93.2%. The gaseous methane production increased from 0.08 ± 0.05 to 0.14 ± 0.04 L/d, and the total methane production rate was enhanced from 0.21 ± 0.11 to 0.23 ± 0.09 LCH4/gCOD. Thus, the treatment performance of the G-AnDMBR was superior to that of the C-AnDMBR, and the addition of GAC could improve the effluent quality during the initial dynamic membrane formation process. In addition, the buffering effect of GAC made the G-AnDMBR maintain a relatively stable solution environment. The G-AnDMBR showed a transmembrane pressure (TMP) increasing rate of 0.045 kPa/d, which was obviously lower than that of the C-AnDMBR (0.057 kPa/d) because the nonfluidized GAC could trap fine sludge particles and adsorb soluble extracellular polymer substances (SEPSs), thus inhibiting the over formation of the dynamic membrane layer. A microbial property analysis indicated that GAC induced a change in the microbial community and enhanced the gene abundance of type IV pili and that it also potentially accelerated the direct interspecific electron transfer (DIET) among syntrophic bacteria and methanogens by enriching specific functional microorganisms. The results indicated that the integration of GAC and the AnDMBR process can be a cost-effective and promising alternative for domestic wastewater treatment and bioenergy recovery.

Feasibility of successive hydrogen and methane production in a single-reactor configuration of batch anaerobic digestion through bioaugmentation and stimulation of hydrogenase activity and direct interspecies electron transfer

Two-stage anaerobic digestion is a promising way to increase the efficiency of decomposition of organic waste. However, spatial separation of stages makes this technology less economically attractive. In this work, we studied a model of two-stage anaerobic digestion with stages separated in time in one reactor. To implement this model, we used a combination of (1) bioaugmentation with a hydrogen-producing Thermoanaerobacterium thermosaccharolyticum SP-H2, resistant to low pH, (2) setting a low initial pH (5.5) to reduce the activity of hydrogenotrophic methanogens, (3) supplementing additives in the form of soluble iron (II) and granular activated carbon (GAC) to stimulate hydrogenase activity and direct interspecies electron transfer (DIET), respectively. For comparison, two methanogenic inoculums with different dominant microbial groups and initial iron concentrations were used. With the simultaneous addition of GAC and iron (II), sequential production of hydrogen and methane was observed for both methanogenic inoculums. Without the addition of GAC, methane formation was practically not observed, which, apparently, indicated the impossibility of syntrophic degradation of acidogenesis products due to the high partial pressure of hydrogen and the absence of conductive material for activation of DIET. Significant differences in hydrogenase activity and the kinetics of hydrogen and methane formation according to the modified Gompertz equation depending on the iron content in the system may indicate the importance of this additive. Further studies should be aimed at determining the optimal initial pH, concentrations of stimulating additives, bioaugmentation and methanogenic cultures, as well as confirming the stability of the proposed single-reactor two-stage anaerobic digestion system in semi-continuous mode.

Granular activated carbon stimulated microbial extracellular secretions in anaerobic digesters: An egalitarian act and beyond

Granular activated carbon (GAC) has been widely studied for its roles in improving anaerobic digestion (AD) through its conductive properties in stimulating direct interspecies electron transfer or its surface functional groups in altering redox conditions in AD. In the present study, the impacts of GAC on the biosynthesis of extracellular microbial secretions (e.g., amino acids and vitamins), and the roles of these secretions in AD were investigated. Four continuous laboratory reactors were operated under ambient temperature (20 ± 0.5 °C) conditions, including two up-flow anaerobic sludge blanket (UASB) bioreactors with GAC addition to one of the UASBs; and two anaerobic sequencing batch reactors (ASBRs) each fed with the centrifuged supernatants (to remove suspended biomass and GAC debris) of UASB effluents supplemented with additional carbon source (glucose). Our finding shows that GAC addition stimulated extracellular microbial secretions in UASB, and these secretions, which can work as essential nutrients, enhanced COD removal (from 48 ± 3 % to 60 ± 1 %) and methane production (31 ± 3 % to 47 ± 5 %) in its downstream ASBR. Interestingly, microbial kinetics test showed that extracellular microbial secretions from the GAC-amended UASB improved methane production rate from 72.5 ± 1.5 to 105.6 ± 1.2 mg CH4-COD/g VSS/d without dramatic microbial community shift. We conclude that the impact of GAC on AD does not only rely on the direct contact of microorganisms and GAC materials, and the GAC-stimulated microbial extracellular secretions represent an important mechanism in conductive material amended AD.

Anaerobic Digestion of Chicken Manure in the Presence of Magnetite, Granular Activated Carbon, and Biochar: Operation of Anaerobic Reactors and Microbial Community Structure

The influence of magnetite nanoparticles, granular activated carbon (GAC), and biochar, as well as their combinations on the anaerobic digestion of chicken manure and the structure of microbial communities was studied. The addition of magnetite, GAC, and biochar increased the rate of methane production and the total methane yield. It has been observed that these additives stimulated anaerobic microorganisms to reduce the concentration of accumulated volatile organic acids. Various bacterial species within the classes Bacteroidia and Clostridia were found at higher levels in the anaerobic reactors but in different proportions depending on the experiment. Members of the genera Methanosarcina, Methanobacterium, Methanothrix, and Methanoculleus were mainly detected within the archaeal communities in the anaerobic reactors. Compared to the 16S rRNA gene-based study, the mcrA gene approach allowed a higher level of Methanosarcina in the system with GAC + magnetite to be detected. Based on our findings, the combined use of granular activated carbon and magnetite at appropriate dosages will improve biomethane production.

Enhanced anaerobic digestion of dairy wastewater in a granular activated carbon amended sequential batch reactor.

This study investigated the potential of granular activated carbon (GAC) supplementation to enhance anaerobic degradation of dairy wastewater. Two sequential batch reactors (SBRs; 0.8 L working volume), one control and another amended with GAC, were operated at 37°C and 1.5–1.6 m/h upflow velocity for a total of 120 days (four cycles of 30 days each). The methane production at the end of each cycle run increased by about 68%, 503%, 110%, and 125% in the GAC‐amended SBR, compared with the Control SBR. Lipid degradation was faster in the presence of GAC. Conversely, the organic compounds, especially lipids, accumulated in the absence of the conductive material. In addition, a reduction in lag phase duration by 46%–100% was observed at all four cycles in the GAC‐amended SBR. The peak methane yield rate was at least 2 folds higher with GAC addition in all cycles. RNA‐based bacterial analysis revealed enrichment of Synergistes (0.8% to 29.2%) and Geobacter (0.4% to 11.3%) in the GAC‐amended SBR. Methanolinea (85.8%) was the dominant archaea in the biofilm grown on GAC, followed by Methanosaeta (11.3%), at RNA level. Overall, this study revealed that GAC supplementation in anaerobic digesters treating dairy wastewater can promote stable and efficient methane production, accelerate lipid degradation and might promote the activity of electroactive microorganisms.

Alleviation of ammonia inhibition in dry anaerobic digestion of swine manure

The effects of granular activated carbon (GAC) and organic load rate (OLR) on semi-continuous dry anaerobic digestion of swine manure were investigated. A kinetic model of anaerobic digestion provided a good description of the quantitative effect of OLR on the methane production rate, with a maximum methane production rate of 1.46 and 1.60 LCH4/L/d without and with addition of GAC, respectively; this rate reached approximately 80% of the reported maximum methane production rate for the wet anaerobic digestion of swine manure, thus suggesting favorable application prospects of dry anaerobic digestion of swine manure. With an increase in OLR from 1.0 to 5.0 g TS/L/d, the ammonia nitrogen concentration increased from 3433 to 4094 mg/L; after the OLR reached 7.0 g TS/L/d, the ammonia nitrogen concentration decreased, to 3654 mg/L at 7.0 g TS/L/d and 2324 mg/L at 9.0 g TS/L/d, and ammonia inhibition was alleviated. The improvement in the biogas production efficiency with the addition of GAC was apparent at the start-up stage and under high OLR (≥7.0 g TS/L/d).

Enhancing the resistance to H2S toxicity during anaerobic digestion of low-strength wastewater through granular activated carbon (GAC) addition

Low-strength wastewater was treated using two laboratory-scale up-flow anaerobic sludge blankets (UASB) for 130 days under sulfate-reducing conditions. Granular activated carbon (GAC) was added to one of the reactors. The GAC addition increased the total chemical oxygen demand removal by 21–28% and total methane production by 32–78%. The sludge from the GAC-amended UASB showed higher specific methanogenic activities (SMA) and higher activities in the presence of H2S, indicating that the GAC addition enhanced the resistance of methanogens to H2S toxicity. Further, the microbial communities showed that the GAC addition shifted microbial communities. A robust syntrophic partnership between bacteria (i.e., Bacteroidetes_vadinHA17 and Trichococcus) and methanogens was established in the GAC-amended UASB. Sulfate-reducing bacteria (SRB) were enriched in the GAC biofilm, indicating the coexistence of competition and cooperation between SRB and methanogens. These findings provide significant insights regarding microbial community dynamics, especially SRB and methanogens, in a GAC-amended anaerobic digestion process under sulfate-reducing conditions.

Enhanced biological phosphorus removal in aerobic granular sludge reactors by granular activated carbon dosing

This study investigated the effects of granular activated carbon (GAC) addition on the enrichment of polyphosphate accumulating organisms (PAOs), stratification of PAOs in the co-existing GAC-biofilms and granules and biological nutrient removal (BNR) in aerobic granular sludge (AGS) reactors. It was found that BNR increased in the GAC-augmented system. Establishment of enhanced biological phosphorus removal (EBPR) pathway was faster with about 1.7 to 2-fold higher P removal in GAC system than control. EBPR biomass grown in the presence of GAC was segregated into different size fractions for determining BNR and stratification of microbial groups. It was found that EBPR was majorly associated with the large biomass (>0.5 mm) fraction, corroborating with higher abundance of PAOs. Higher P removals of 60 to 70% with characteristic EBPR profiles were observed in 0.5 mm fraction. In contrast, P removals by 0.25 mm fraction were lower at 20 to 35% without EBPR profiles. EBPR biomass (>0.5 mm) fraction was segregated into granules and GAC-biofilms for determining the role of GAC in PAOs enrichment. P release (2.5–3.5 mg L−1 P) and P uptake (5–7 mg L−1 P) were higher in the P removal profiles exhibited by GAC-biofilms. In contrast, P release and P uptake were lower with the granules. These differences in P removal profiles resulted in distinct net P removal efficiencies of 70 ± 5% and 50 ± 6% for GAC-biofilms and granules, respectively. These differences in P removals were corroborated by higher abundance of PAOs in the GAC-biofilms than co-existing granules. PAO clade-level enrichment was found to be dependent on substrate wherein acetate feeding enriched PAO clade I, while acetate-propionate feeding caused enrichment of both PAO clade I and II. These results suggest that GAC addition to AGS reactors can aid in enrichment of PAOs, reduce the start-up period for EBPR, and increase P removal efficiencies.

Enhancing robustness of halophilic aerobic granule sludge by granular activated carbon at decreasing temperature

High salinity seriously inhibits the growth and metabolism of microorganisms, resulting in poor settleability, excessive biomass loss and low treatment efficiency of biological wastewater treatment systems. The development of halophilic aerobic granular sludge (HAGS) is a feasible strategy for addressing this challenge. However, there are problems with the granulation of HAGS and the stability of granules at decreasing temperatures. In this study, granular activated carbon (GAC) with a large specific surface area and good biocompatibility was used to enhance the robustness of HAGS. The results showed that the addition of GAC shortened the granulation time from 60 d (control system) to 35 d (GAC-addition system). The proteins contents of extracellular polymeric substances (EPS) in the GAC-addition system was significantly higher (p < 0.05) than that in the control system during granulation. Satisfactory NH4+-N and chemical oxygen demand (COD) removal efficiencies reached more than 96% in both systems at 18–26 °C. When the operating temperature was lower than 15 °C, the GAC-addition system exhibited better NH4+-N removal performance (>80%) than the control system (<60%). Moreover, the abundance of almost all nitrogen metabolism-related genes in the GAC-addition system was higher than that in the control system. During the granulation process, the enrichment of functional microorganisms, including family Flavobacteriaceae, Rhodobacteraceae, and Cryomorphaceae, may promote the production of EPS by significantly upregulating (p < 0.05) the metabolic pathway “Signaling Molecules and Interaction” in the GAC-addition system. The overexpression of the nitrogen assimilation gene glnA in heterotrophic bacteria (Halomonas and Marinobacterium) may promote the conversion of inorganic nitrogen to extracellular proteins to adapt to the decreased operational temperature. Our findings confirm that GAC addition is a simple but effective strategy to accelerate granulation and enhance the robustness of HAGS in saline wastewater treatment.

Promotion of granular activated carbon on methanogenesis of readily acidogenic carbohydrate-rich waste at low inoculation ratio

Although granular activated carbon (GAC) supplementation into food waste anaerobic digestion system is an efficient means to enhance methane production. As yet, little is known whether GAC supplementation is suitable for the extreme condition of pH below 4.5, which occurs in the use of readily acidogenic carbohydrate-rich waste (RACW) as methanogenic substrate when at low inoculation/substrate (I/S) ratio. This study investigated the effects of GAC on RACW anaerobic digestion under different inoculation/substrate (I/S) ratios. It was found that the addition of GAC was a preferred alternative method to enhancing I/S ratio for promoting methane production from RACW. The additive dose of 20 g/L was recommended for the methanogenesis of RACW at low I/S of 1:2, and the methane yield was enhanced by 12 times (505 mL/g-VS) compared with that (42 mL/g-VS) from the control. This promotion resulted from the apparently solving the over-acidogenesis problem and the adjustment of pH to the desired range. Further investigation revealed that the added GAC enhanced the activities of acetate kinase and coenzyme F420, that engaged in the acidogenic and methanogenic reactions. Meanwhile, the decrease of hydrogenase and increase of c-Cyts implied that the metabolism of direct interspecies electron transfer (DIET) was probably stimulated by GAC. Microbial investigation inferred that the enriched hydrogenotrophic methanogens and DIET-mediated syntrophic partners of Geobacter/Syntrophomonas with Methanosarcina were responsible for the enhanced methane yield.