Addition Of External Carbon Source Research Articles

A Continuous Plug-Flow Anaerobic-Multistage Anoxic/Aerobic Process Treating Low-C/N Domestic Sewage: Nutrient Removal, Greenhouse Gas Emissions, and Microbial Community Analysis

The anaerobic-multistage anoxic/aerobic (A-MAO) process has shown good potential for advanced nitrogen removal in recent years, but its greenhouse gas emissions still need to be fully explored. The effects of the influent distribution and external carbon source sodium acetate on nutrient removal, greenhouse gas emissions, and the microbial community structure in a continuous plug-flow A-MAO reactor fed with real low C/N ratio domestic sewage were investigated. The results showed that altering the allocation of carbon source resulted in average chemical oxygen demand (COD) and total nitrogen (TN) concentration in effluent reduced to 26.10 ± 4.86 and 6.65 ± 1.73 mg/L, respectively. Both operations reduced the emission rate of greenhouse gas. While the addition of external car-bon sources leaded to lower N2O emission rates and higher CO2 and CH4 emission rates. The addition of sodium acetate facilitated nitrification and denitrification processes, thereby leading to a reduction in N2O production. Meanwhile, it spurred the growth of methanogenic bacteria and heterotrophic microorganisms, thus boosting the production of CO2 and CH4. Influent distribution promoted the increase of Bacteroidota, Chloroflexi and Acidobacteriota of the reactor. The enrichment of typical hydrolytic bacteria and glycogen accumulating organisms (GAOs) increased the utilization efficiency of carbon sources in the system after the addition of sodium acetate. The significant increase of typical denitrifying bacteria (DNBs) Azospira reduced the N2O emission during heterotrophic denitrification process, which was considered to be an important functional genus for increasing nitrogen loss in this system. The rational utilization of carbon source makes the difference in metabolism function. The study provides a valuable strategy for comprehensively evaluating the pollutant removal and greenhouse gas emission reduction from the A-MAO process.

Recent advancements in harnessing biodiesel from microalgae through attached growth systems

Conventional microalgal cultivation method relied on suspended growth or attached growth that uses inert solid surfaces, requiring the addition of external carbon sources to promote microalgal growth. The inert solid substrates for attached microalgae pose constraints on the maximal capabilities of these microorganisms as it does not possess the ability to provide the necessary nutritional support required for optimal microalgal growth. As a result, microalgae are obliged to only rely on the solid substrate as a means of attachment, without any further nutritional provisions. Slower growth rates, larger resource requirements, and exorbitant operating expenses had prompted the exploration of an alternative strategy, i.e; the innovative use of solid organic waste as nutritional source cum attachment platform. The findings of this review unveil key insights into microalgae cultivation system, offering a solution to economic challenges in the production phase. This innovation not only promotes economic feasibility and sustainability, but also contributes to economically viable and environmentally sustainable biofuel production.

Interaction of reed litter and biochar presences on performances of constructed wetlands

Constructed wetlands (CWs) are frequently used for effective biological treatment of nitrogen-rich wastewater with external carbon source addition; however, these approaches often neglect the interaction between plant litter and biochar in biochar-amended CW environments. To address this, we conducted a comprehensive study to assess the impacts of single or combined addition of common reed litter and reed biochar (pyrolyzed at 300 and 500 °C) on nitrogen removal, greenhouse gas emission, dissolved organic matter (DOM) dynamics, and microbial activity. The results showed that combined addition of reed litter and biochar to CWs significantly improved nitrate and total nitrogen removal compared with biochar addition alone. Compared to those without reed litter addition, CWs with reed litter addition had more low-molecular-weight and less aromatic DOM and more protein-like fluorescent DOM, which favored the enrichment of bacteria associated with denitrification. The improved nitrogen removal could be attributed to increases in denitrifying microbes and the relative abundance of functional denitrification genes with litter addition. Moreover, the combined addition of reed litter and 300 °C-heated biochar significantly decreased nitrous oxide (30.7 %) and methane (43.9 %) compared to reed litter addition alone, while the combined addition of reed litter and 500 °C-heated biochar did not. This study demonstrated that the presences of reed litter and biochar in CWs could achieve both high microbial nitrogen removal and relatively low greenhouse gas emissions.

Autotrophic denitrification based on sulfur-iron minerals: advanced wastewater treatment technology with simultaneous nitrogen and phosphorus removal.

Autotrophic denitrification technology has many advantages, including no external carbon source addition, low sludge production, high operating cost efficiency, prevention of secondary sewage pollution, and stable treatment efficiency. At present, the main research on autotrophic denitrification electron donors mainly includes sulfur, iron, and hydrogen. In these autotrophic denitrification systems, pyrite has received attention due to its advantages of easy availability of raw materials, low cost, and pH stability. When pyrite is used as a substrate for autotropic denitrification, sulfide (S2-) and ferrous ion (Fe2+) in the substrate will provide electrons to convert nitrate (NO3-) in sewage first to nitrite (NO2-), then to nitrogen (N2), and finally to discharge the system. At the same time, sulfide (S2-) loses electrons to sulfate (SO42-) and ferrous ion (Fe2+) loses electrons to ferric iron (Fe3+). Phosphates (PO43-) in wastewater are chemically combined with ferric iron (Fe3+) to form ferric phosphate (FePO4) precipitate. This paper aims to provide a detailed and comprehensive overview of the dynamic changes of nitrogen (N), phosphorus (P), and other substances in the process of sulfur autotrophic denitrification using iron sulfide, and to summarize the factors that affect wastewater treatment in the system. This work will provide a relevant research direction and theoretical basis for the field of sulfur autotrophic denitrification, especially for the related experiments of the reaction conversion of various substances in the system.

Simultaneous Mixotrophic Nitrate Removal and Phosphorus Removal in a Sponge-Iron Denitrifying Filter

Due to stricter municipal wastewater discharge standards, there is an increased need for further treatment of nitrate in the secondary effluent of wastewater treatment plants. This is achieved through denitrification by the addition of external carbon sources, which leads to increased costs in wastewater treatment. The aim of this study was to examine the possibility of simultaneous removal of nitrate and phosphorus from simulated secondary effluent by employing a sponge-iron-based denitrifying filter at room temperature. The results indicate that at hydraulic retention times of over 2 h, more than 60% of the nitrate was reduced to ammonia and nitrite via iron-based abiotic nitrate reduction. However, sponge iron easily scaled after two months of operation. Therefore, a little glucose was added to the influent, resulting in a final COD/N ratio of 1:1. Mixotrophic nitrate reduction was observed, and the rust of sponge iron was successfully dissolved. Batch test results demonstrate that biological nitrate denitrification accounted for 70.0% of the total nitrate reduction. Additionally, high-efficiency phosphorus removal through the chemical reaction of released iron and phosphorus was achieved throughout the entire experiment, with removal efficiencies of more than 90% at hydraulic retention times of over 2 h. Moreover, high-throughput sequencing data show that the species diversity obviously increased after adding organic carbon, suggesting the coexistence of heterotrophic and autotrophic denitrifiers. Hence, the sponge-iron denitrifying filter has considerable prospects in the field of secondary effluent treatment and is likely to be the future direction of zero-valent iron application in sewage treatment.

A step toward a biofloc system with no‐carbon addition: Increasing dietary lipid level enhances the water quality and body chemical composition without affecting tilapia health status

AbstractA 70‐day experiment was carried out to evaluate biofloc system operation with no carbon addition by manipulating dietary protein and lipid levels for Nile tilapia (Oreochromis niloticus) cultivation. Three treatments were conducted; Control treatment (C; fed on a 30% crude protein [CP] diet supplemented with 3% vegetable oil [VO] + addition of external carbon source [ECS] in rearing tank), low protein–low lipid treatment (LPLL; fed on 23% CP diet supplemented with 3% (VO) + no addition of ECS) and low protein–high lipid treatment (LPHL; fed on 23% CP diet supplemented with 9% VO + no addition of ECS). The C treatment recorded the highest values (p < 0.05) in terms of final body weight (FBW), specific growth rate, floc volume, total suspended solids, and ammonia nitrogen. No significant differences were observed between C and LPHL treatments regarding FBW and protein efficiency ratio. The liver sections of LPHL treatment showed better values for histological alteration index compared to the control. It could be concluded that applying a biofloc system with no carbon addition using LPHL diets may afford better water quality conditions, limit the environmental impact and decrease dietary costs with limited effect on fish performance.

Groundwater denitrification using a continuous flow mode hybrid system combining a hydrogenotrophic biofilter and an electrooxidation cell

An attached-growth continuous flow hydrogenotrophic denitrification system was investigated for groundwater treatment. Two bench-scale packed-bed reactors were used in series, without external pH adjustment or carbon source addition, while inorganic carbonate salts already contained in the groundwater were the sole carbon source used by the denitrifying bacteria. The hydrogen was produced by water electrolysis using renewable energy sources thus minimizing resource-draining factors of the treatment process. The biofilter was subjected to a combination of three groundwater retention times (13.5, 27 and 54 min, corresponding to 20, 10 and 5 mL min−1 inlet water flow rates) and two hydrogen flow values (10 and 20 mL min−1) to evaluate its efficiency under different operating parameters. In all cases, significant nitrate percentage removals were achieved, ranged between 64.1% and 100%. The treatment process appears to slow down with lower retention times and H2 flow rate values, although residual nitrate concentrations were always in the range of 0–5.1 mg L−1, values below the maximum permitted limit of 11.3 mg L−1. In cases where nitrite accumulation was detected, a continuous flow electrochemical oxidation process with three different current density values (5.0, 7.5 and 10.0 mA cm−2) was examined as a post-treatment step aiming to completely remove the toxic nitrite anions. Finally, an advanced mathematical model of the attached growth hydrogenotrophic denitrification process was developed to predict concentrations of all the substrates examined in the bio-filter (nitrate, nitrite, inorganic carbon and hydrogen).

Application of Deep-bed Denitrification Filter in Sewage Treatment

The combined process of A2/O oxidation ditch, high efficiency sedimentation tank and denitrification deep bed filter was adopted in Sewage Treatment Plant of Hefei City to treat municipal domestic sewage. The effluent quality could meet the discharge limit of major water pollutants in urban sewage treatment plants and industrial industries of Chaohu Lake Basin (DB34/2710-2016). In addition to the parameters of design and operation, the effect of SS and deep denitrification was summarized in detail in this paper. The results showed that the combined process SS and denitrification effect was removed very well. The operational results demonstrated SS removal efficiency 74.3%,TN removal efficiency (92.3％) in the biosystem ,in which 80.0％ and 12.3％ of TN in influent were removed in the pre-denitrif ication in oxidation ditch and post-denitrification in deep bed denitrification filter, respectively. The deep bed denitrification filter could be used as the safeguard for effluent TN with the addition of external carbon source to ensure the efficiency of TN less than 5 mg/L.

Systematic review: External carbon source for biological denitrification for wastewater.

Nitrogen mitigation is serious environmental issue around the globe. Several methods for wastewater treatment have been introduced, but biological denitrification has been recommended, particularly with addition of the best external carbon source. The key sites of denitrification are wetlands; it can be carried out with different methods. To highlight the aforementioned technology, this paper deals to review the literature to evaluate biological denitrification and to demonstrate cost effective external carbon sources. The results of systematic review disclose the denitrification process and addition of different external carbon sources. The online literature exploration was accomplished using the most well-known databases, that is, science direct and the web of science database, resulting 625 review articles and 3084 research articles, published in peer-reviewed journals between 2015 and 2021 were identified in first process. After doing an in-depth literature survey and exclusion criteria, we started to shape the review from selected review and research articles. A number of studies confirmed that both nitrification and denitrification are significant for biological treatment of wastewater. The studies proved that the carbon source is the main contributor and is a booster for the denitrification. Based on the literature reviewed it is concluded that biological denitrification with addition of external carbon source is cost effective and best option in nitrogen mitigation in a changing world. Our study recommends textile waste for recovery of carbon source.

Industrial wastewater as a source of external organic carbon for the biological nutrient removal.

Addition of external organic carbon source for denitrification is generally used in wastewater treatment plants (WWTPs) to intensify nitrogen removal processes. The aim of the laboratory survey was to measure the composition of concentrated industrial wastewater, determine the possibilities of its use as an external denitrification substrate, and assess its overall impact on WWTP. The obtained results demonstrate that the analysed industrial wastewater is biodegradable, and can be used as a denitrification substrate without special adaptation of biomass. The denitrification rates with tested wastewater were in the range of 1.6 to 1.9mgN/g·h. Negative influence of long-term dosing of industrial wastewater on activated sludge were not confirmed. The effect of imported wastewater on WWTP must be assessed comprehensively, including the impact of heavy metals from wastewater on the sludge quality. The instructions on how to calculate this balance are provided in the article.

Enhancement of partial denitrification-anammox pathways in constructed wetlands by plant-based external carbon sources

Insufficient carbon source/electron donor often limits the denitrification efficiency of constructed wetlands (CWs) when treating wastewater characterized by low carbon-to-nitrogen ratios. Hence, the viability of wetland plant-based carbon sources for enhanced nitrogen removal and its mechanisms in horizontal subsurface flow CWs was assessed. Results showed cattails to have a better carbon release capacity than wheat straw and corn cob. Alkali pretreatment of cattails is demonstrated to significantly enhance the release of volatile fatty acids (68.31 ± 1.36 mg/g) dominated by acetic acid to sustain a high denitrification rate (0.62 ± 0.05 mg/(L·h)) over a long duration. External carbon source addition to adjusting C/N ratio to 3.5 achieved the highest removal rates of 75.45%, 91.33% and 76.03%, respectively, for total nitrogen (TN), ammonia-nitrogen (NH4+-N) and nitrate-nitrogen (NO3−-N). High-throughput sequencing showed plant-based external carbon sources to significantly increase the relative abundance of the denitrifying bacteria, as well as the anammox bacteria. Moreover, it elevated the number of nitrate reductase relative to nitrite reductase by 4.65-fold. Plant-based external carbon sources enhanced the partial denitrification-anammox (PDN/AMX) contribution to TN removal by at least 27.29% and improved nitrogen removal efficiency through co-promotion of denitrification and PDN/AMX pathways. Findings from this study support the utilization of plant-based external carbon sources in CWs. Additionally, it provides new insights into enhancement pathways of nitrogen removal with plant-based carbon sources.

A Study on the change of nitrogen removal characteristics in the applications of external carbon sources using organic waste resources

Waste water emitted from industrial facilities is increasing in relative inorganic content along with a decrease in the amount of organic matter every year, making it difficult to remove nutrients caused by high concentrations of organic matter in the early stages of wastewater treatment. In the wastewater treatment process, the denitrification process for nitrogen removal requires organic carbon as an electronic donor, so the addition of external carbon sources is necessary, but there is a concern of increased cost, obstruction of nitrification of residual organic carbon sources. The external carbon sources used for denitrification can include methanol, ethanol, and wastewater, which have been studied in various ways. However, failure to properly use these sources of external carbon can lead to a decrease in denitrification efficiency, which can lead to increased COD(Chemical Oxygen Demand), resulting in deterioration of water quality as well as a decrease in nitric oxide efficiency. Therefore, the purpose of this study was to investigate the nitrogen removal characteristics of wastewater when applying wastewater, methanol, and antibiotic-announced wastewater used as an external carbon source. This study was conducted in a biological diffuse reaction tank, and the KNO3 reagent was injected to 1.4 mg/L within the reaction tank to see the denitrification efficiency, and methanol, A negative wastewater, B negative waste water, and antibiotic fermented waste were injected in the same amount. Methanol, primary treated water, and antibiotic fermented waste reduced the total solids concentration of sludge in bioreactors by 5.2%, 17.4% and 3.4%, respectively, but the total solids concentration of A food wastewater and H food wastewater was increased by 9.1% and 8.3%, respectively. In addition, the concentration of T-N(Total Nitrogen) in bio-reactor treated water decreased by 15.6% and 0.97% and 0.85%, respectively, when methanol, primary treatment water and antibiotic fermented waste liquid were injected, but T-N in A food wastewater and H food wastewater negative waste water increased by 6.7% and 2.9%, respectively. The concentration of NO3--N in methanol, primary treated water, antibiotic fermented waste solution, A negative wastewater, and H negative wastewater was reduced by 56.4%, 9.95%, 0.85%, 11.8% and 18.3%, respectively. If organic waste resources are applied as external carbon sources in the wastewater treatment process, it is possible to supply insufficient carbon sources during the denitrification process, but the nitrogen concentration of organic waste resources can rather increase the concentration of T-N. Further research on the possibility of decomposition of nitrogen sources contained in drinking water and antibiotic fermented waste liquid in the wastewater treatment process will be conducted through continuous experiments by type of organic waste resources.

Effects of applying different carbon substrates on nutrient removal and greenhouse gas emissions by constructed wetlands treating carbon-depleted hydroponic wastewater

The addition of external carbon sources is crucial for effective biological treatment of nutrient-rich but carbon-depleted hydroponic wastewater using constructed wetlands. In this study, we examined the effects of applying three types of carbon substrates, namely sucrose, hydroponic kale residues, and common reed litter, on the nutrient removal efficiency and greenhouse gas emission rate of vertical flow constructed wetlands. The addition of sucrose and common reed litter was shown to perform equally well in enhancing the removal of total nitrogen (84.9–93.5%), nitrate (98.3–99.8%) and phosphate (53.8–55.2%) as compared to the control. Moreover, the application of common reed litter led to significantly lower mean CH4 and N2O emissions than that of kale residues. These findings suggested that Phragmites reed litter, which is easily found in wetlands worldwide, could be an effective, low-cost and climate-friendly carbon substrate to be applied in constructed wetlands for hydroponic wastewater treatment.

Modelling the effect of SMP production and external carbon addition on S-driven autotrophic denitrification

The aim of this study was to develop a mathematical model to assess the effect of soluble microbial products production and external carbon source addition on the performance of a sulfur-driven autotrophic denitrification (SdAD) process. During SdAD, the growth of autotrophic biomass (AUT) was accompanied by the proliferation of heterotrophic biomass mainly consisting of heterotrophic denitrifiers (HD) and sulfate-reducing bacteria (SRB), which are able to grow on both the SMP derived from the microbial activities and on an external carbon source. The process was supposed to occur in a sequencing batch reactor to investigate the effects of the COD injection on both heterotrophic species and to enhance the production and consumption of SMP. The mathematical model was built on mass balance considerations and consists of a system of nonlinear impulsive differential equations, which have been solved numerically. Different simulation scenarios have been investigated by varying the main operational parameters: cycle duration, day of COD injection and quantity of COD injected. For cycle durations of more than 15 days and a COD injection after the half-cycle duration, SdAD represents the prevailing process and the SRB represent the main heterotrophic family. For shorter cycle duration and COD injections earlier than the middle of the cycle, the same performance can be achieved increasing the quantity of COD added, which results in an increased activity of HD. In all the performed simulation even in the case of COD addition, AUT remain the prevailing microbial family in the reactor.

A novel partial denitrification, anammox-biological phosphorus removal, fermentation and partial nitrification (PDA-PFPN) process for real domestic wastewater and waste activated sludge treatment

A novel process was developed for real domestic wastewater and waste activated sludge (WAS) treatment based on partial denitrification, anammox-biological phosphorus removal, fermentation and partial nitrification (PDA-PFPN). After 246 days of operation, the effluent concentrations of NH4+-N, NO2−-N and NO3−-N were below detection limits (0.1 mg/L), and the effluent concentration of PO43−-P was 0.1 mg/L without the addition of external carbon source in PDA-PFPN system. Moreover, the sludge reduction efficiency reached 48.1% due to fermentation. The nitrite accumulation ratios by ammonia oxidation and nitrate reduction pathway were 60.6% and 87%, respectively. Intracellular metabolites measured by liquid chromatography mass spectrometer (LC-MS/MS) suggested that different intracellular amino acids were stored and consumed at different duration, and intracellular Valine, Glycine and Lysine were not utilized in oxic stage. Results of flow cytometry showed that the proportion of intact cells decreased from 94.7% to 82.9%, and necrotic cells increased from 5.3% to 17.1% with the increase of DNA content in sludge supernatant and cell decay rate, indicating the occurrence of cell death and lysis and leading to WAS reduction. Analysis of transcriptional community composition revealed that partial denitrification bacteria (Thauera), anammox bacteria (Candidatus Brocadia and Candidatus Kuenenia), simultaneous phosphorus removal and fermentation bacteria (Tetrasphaera) and partial nitrification bacteria (Nitrosomonas) coexisted and actually worked in PDA-PFPN system. The novel PDA-PFPN process simultaneously achieved highly efficient nitrogen and phosphorus removal and WAS reduction without the addition of external carbon source, which greatly reduced the operation cost of carbon source dosing and WAS treatment in wastewater treatment.

Mainstream nitritation and denitritation in a pilot-scale multi-cycle sequencing batch reactor

Addition of external carbon sources for deep nitrogen removal highly increased the cost of municipal wastewater treatment. In this study, a carbon-saving nitritation and denitritation process was developed in a pilot-scale multi-cycle sequential batch reactor over 230 days. In the first three stages, the effluent total nitrogen (TN) concentration of the pilot plant without external carbon addition was similar to that of the on-site cyclic activated sludge system with external carbon addition. A highest nitrite accumulation rate (57.3%) and TN removal rate (85.1%) was observed, respectively, under conditions that temperature of 28.3 °C, dissolved oxygen concentrations of 2.0 mg/L and COD/N ratios of 4.5:1 as well as a short oxic phase (32.8 min). As the influent TN decreased after heavy rain, NOB could still be suppressed by shortening oxic phase and lowering DO (1.5 mg/L). In winter, slight nitrite accumulation was still obtained by adding glucose in stage IV. Nitrite competition between NOB and denitrifiers was indeed the key to NOB suppression. It should be a good choice for wastewater treatment plants to achieve high discharge standard and save carbon source using such a proposed process.

Initial carbon release characteristics, mechanisms and denitrification performance of a novel slow release carbon source

External carbon source addition is one of the effective methods for the treatment of wastewater with low carbon to nitrogen ratio (C/N). Compared with fast-release liquid carbon sources, slow-release solid carbon sources are more suitable for the denitrification process. A novel slow-release solid carbon source (corncob-polyvinyl alcohol sodium alginate- poly-caprolactone, i.e. CPSP) was prepared using corn cob (CC) and poly-caprolactone with polyvinyl alcohol sodium alginate as hybrid scaffold. The physical properties and carbon release characteristics of CPSP and three other carbon sources were compared. CPSP had stable framework and good carbon release performance, which followed the second order release equation. The formic acid, acetic acid, propionic acid and butyric acid released from CPSP accounted for 8.27% ± 1.66 %, 56.48% ± 3.71 %, 18.46% ± 2.69% and 16.79% ± 3.02% of the total released acids respectively. The start-up period of CPSP was shorter than that of the other carbon sources in denitrification experiment, and no COD pollution was observed in the start-up phase (25–72 h) and stable phase (73–240 hr). The composition and structure of the dissolved organic compounds released by CPSP and other carbon sources were analyzed by UV-Vis absorption spectroscopy and three-dimensional fluorescence spectroscopy, which indicated that CPSP was more suitable for denitrification than the other studied carbon sources. [Display omitted]

Nitrogen resource recovery from mature leachate via heat extraction technology: An engineering project application.

A large pool of ammonia in mature leachate is challenging to treat with a membrane bioreactor system to meet the discharge Standard for Pollution Control on the Landfill Site of Municipal Solid Waste in China (GB 16889-2008) without external carbon source addition. In this study, an engineering leachate treatment project with a scale of 2,000 m3/d was operated to evaluate the ammonia heat extraction system (AHES), which contains preheat, decomposition, steam-stripping, ammonia recovery, and centrifuge dewatering. The operation results showed that NH3-N concentrations of raw leachate and treated effluent from an ammonia heat extraction system (AHES) were 1,305-2,485 mg/L and 207-541 mg/L, respectively. The ratio of COD/NH3-N increased from 1.40-1.84 to 7.69-28.00. Nitrogen was recovered in the form of NH4HCO3 by the ammonia recovery tower with the introduction of CO2, wherein the mature leachate can offer 37% CO2 consumption. The unit consumptions of steam and power were 8.0% and 2.66 kWh/m3 respectively, and the total operation cost of AHES was 2.06 USD per cubic metre of leachate. These results confirm that heat extraction is an efficient and cost-effective technology for the recovery of nitrogen resource from mature leachate.

Comparative Study on Advanced Nitrogen Removal of Landfill Leachate Treated by SBR and SBBR

In order to achieve advanced nitrogen removal from landfill leachate without the addition of external carbon sources, a Sequencing Batch Reactor (SBR) and a Sequencing Biofilm Batch Reactor (SBBR) were proposed for the treatment of actual landfill leachate with ammonia nitrogen (NH4+-N) and chemical oxygen demand (COD) concentrations of 1000 ± 100 mg/L and 4000 ± 100 mg/L, respectively. The operating modes of both systems are anaerobic–aerobic–anoxic. After 110 days of start-up and biomass acclimation, the effluent COD and the total nitrogen (TN) of the two systems were 650 ± 50 mg/L and 20 ± 10 mg/L, respectively. The removal rates of COD and total nitrogen could reach around 85% and above 95%, respectively. Therefore, advanced nitrogen removal was implemented in landfill leachate without adding any carbon sources. After the two systems were acclimated, nitrogen removing cycles of SBR and SBBR were 24 h and 20 h, respectively. The nitrogen removing efficiency of SBBR was improved by 16.7% in comparison to SBR. In the typical cycle of the two groups of reactors, the nitrification time of the system was the same, which was 5.5 h, indicating that although the fiber filler occupied part of the reactor space, it had no significant impact on the nitrification performance of the system. At the end of aeration, the internal carbon source content of sludge of SBBR was equivalent to that of the SBR system. However, the total nitrogen concentration of SBBR was only 129 mg/L, which is 33.8% lower than that of SBR at 195 mg/L. The main reason was that biofilm enhanced the simultaneous nitrification and denitrification (SND) effect of the system.

Enhancement of denitrification efficiency using municipal and industrial waste fermentation liquids as external carbon sources

The addition of external carbon source for nitrogen removal from wastewater is an essential step in wastewater treatment. In this study, various external carbon sources from the fermentation of primary sludge (PS), thickened waste activated sludge (TWAS), food waste (FW), bakery processing & kitchen waste (BP + KW), fat, oil, & grease (FOG), and whey powder (WP) were successfully employed for wastewater denitrification. Methanol and acetate were also used as controls due to their common use as external carbon sources for wastewater denitrification. The denitrification performance and kinetics such as the specific denitrification rate (SDNR), denitrification potential (PDN), and the biomass yield were studied at a constant TVFA as COD/N ratio of 5 for all substrates. Complete denitrification was achieved with a NO3−-N removal efficiency of 98–99%, and no NO2− accumulation was observed at the end of the experiments for all substrates. The results revealed that the liquid fermentation filtrates exhibited higher SDNRs than methanol and acetate. This indicates the high organic matter utilization efficiency and better denitrification ability of fermentation filtrates over conventional carbon sources. WP exhibited the highest SDNR of 17.6 mg NOx − N/g VSS/h, which is approximately four times that of methanol (4.6 mg NOx − N/g VSS/h). The other carbon sources had SDNRs two to three times higher than that of methanol. However, the fermentation filtrates exhibited higher biomass yields of 0.26–0.37 mg VSS/mg COD compared to methanol of 0.21 mg VSS/mg COD, which could lead to higher sludge handling costs. Moreover, methanol exhibited higher PDN of 0.25 g N/g COD compared to all the fermentation filtrates.