Addition Of Chlorophyll Research Articles

In Situ Release of Ulvan from Crosslinked Ulvan/Chitosan Complex Films and Their Evaluation as Wound Dressings.

When a wound forms due to any injuries, it should be covered with a functional wound dressing for accelerating wound healing and reducing infection. In this study, crosslinked ulvan/chitosan complex films were prepared with or without the addition of glycerol and chlorophyll, and their wound healing properties were evaluated for potential application in wound dressing. The results showed that the tensile strength and elongation at break of the prepared ulvan/chitosan complex films were 2.23-2.48 MPa and 83.8-108.5%, respectively. Moreover, their water vapor transmission rates (WVTRs) were in the range of 1791-2029 g/m2-day, providing suitable environment for wound healing. Particularly, these complex films could release ulvan in situ in a short time, and the film with chlorophyll added had the highest release rate, reaching 62.8% after 20 min of releasing. In vitro studies showed that they were biocompatible toward NIH 3T3 and HaCaT cells, and promoted the migration of NIH 3T3 cells. These complex films could protect HaCaT cells from oxidative damage and reduce the production of reactive oxygen species (ROS); the addition of chlorophyll also effectively reduced the inflammatory response induced by LPS as found in the reduction in both NO and IL-6. Animal models showed that the complex films added with glycerol and chlorophyll could promote wound healing in the early stage, while accelerating the regeneration of dermal glands and collagen production. Briefly, these ulvan/chitosan complex films had good physiochemical properties and biological activity, and could accelerate wound healing both in vitro and in vivo.

Morphology and Optical Properties Analysis of Cu2+ Doped ZnO for Preparation Dye Sensitized Solar Cell (DSSC)

This research reported Cu2+ un-doped (pure) and doped ZnO semiconductors with variations in Cu2+ concentrations of 3%, 4% and 5% through the sol gel method which aims to determine the morphology and optical properties of ZnO has been investigated. In addition, ZnO film was coated using the doctor blade method with the addition of chlorophyll as a dye sensitizer. Morphological and elemental content tests were carried out using SEM and EDS. The optical properties were analyzed by taking Transmittance data using a UV-Vis Spectrophotometer. SEM analysis with 9900X magnification showed that all samples had small grain sizes and the pores formed were uneven (heterogeneous). The EDS analysis showed that all samples had a higher concentration of zinc by weight than oxygen. UV-Vis Spectrophotometer analysis shows that the transmittance value without dye is lower than using Dye. The addition of Cu2+ concentration affects the morphology and optical properties of ZnO. The higher the addition of Cu2+ added chlorophyll, the higher the absorbance value, so that the transmittance value decreases. The addition of 4% concentration showed the maximum value of chlorophyll as a sensitizer.

Chlorophyll Inhibits the Digestion of Soybean Oil in Simulated Human Gastrointestinal System.

Nowadays, much available processed and highly palatable food such as cream products and fried and convenient food, which usually showed a high energy density, had caused an increase in the intake of dietary lipids, further leading to significant growth in the prevalence of obesity. Chlorophyll, widespread in fruits and vegetables, was proven to have beneficial effects on alleviating obesity. This study investigated the effects of chlorophyll on the digestive characteristics of lipids under in vitro simulated adult and infant gastrointestinal systems. Chlorophyll decreased the release rate of free fatty acid (FFA) during in vitro adult and infant intestinal digestion by 69.2% and 60.0%, respectively. Meanwhile, after gastrointestinal digestion, chlorophyll changed the FFA composition of soybean oil emulsion and increased the particle size of oil droplets. Interestingly, with the addition of chlorophyll, the activity of pancreatic lipase was inhibited during digestion, which may be related to pheophytin (a derivative of chlorophyll after gastric digestion). Therefore, the results obtained from isothermal titration calorimetry and molecular docking further elucidated that pheophytin could bind to pancreatic lipase with a strong affinity of (4.38 ± 0.76) × 107 M−1 (Ka), while the binding site was amino acid residue Trp253. The investigation not only explained why chlorophyll inhibited digestive enzyme activity to reduce lipids digestion but also provided exciting opportunities for developing novel chlorophyll-based healthy products for dietary application in preventing obesity.

Fabrication of mixed matrix membrane containing chlorophyll extracted from spinach for humic acid removal

The implementation of the pore-forming agent in the polymeric membrane has been applied for ages in enhancing the characteristics and performance of mixed matrix membrane. In this work, chlorophyll extracted from spinach has been used as an additional pore-forming agent alongside polyethylene glycol (PEG) to improve the polysulfone's pore structure (PSf) mixed matrix membrane. The effect of chlorophyll was investigated in terms of characteristics and performance of the mixed matrix membrane. As a result, the cross-section morphologies show that the pore structure was enhanced significantly inside the membrane structure for 0.25 wt% chlorophyll concentration, thus resulted in better hydrophilicity, surface roughness, and membrane porosity value. Plus, the permeation and rejection performance was increased drastically due to the pore formation inside the mixed matrix membrane structure. These results prove that the addition of chlorophyll with low concentration managed to improve the characteristics and performance of the fabricated mixed matrix membrane.

Conducting/smart color film based on wheat gluten/chlorophyll/polypyrrole nanocomposite

Abstract In this research biodegradable films based on wheat gluten modified with chlorophyll/polypyrrole (WG/Ch/PPy) were prepared. Some characteristics of synthesized films including; thickness, solubility, water vapor permeability (WVP), moisture, opacity, color, antioxidant, and antimicrobial and conductivity properties were studied. According to the results, by adding chlorophyll and polypyrrole to gluten film, the solubility was significantly decreased. The opacity of films was significantly increased with adding chlorophyll and polypyrrole, where the effect of polypyrrole was higher than chlorophyll. With the addition of chlorophyll and polypyrrole, the antioxidant activity of the films was increased. According to the mechanical properties, the addition of chlorophyll and polypyrrole increased the tensile strength of the films. Also results showed that chlorophyll had no effect on the antibacterial property against Escherichia coli, but PPy enhanced the antibacterial character of gluten film significantly. FT-IR results confirmed the creation of new interaction between gluten, PPy and chlorophyll. SEM results showed that PPy particles (40–100 nm) uniformly covered the film surface. XRD results showed that PPy and chlorophyll decreased the crystalline structure of gluten film. The WG/Ch/PPy film can be used as smart film because of its conductivity change and color change. The changing of film conductivity and film color during storage time of food can represent food quality.

Sesame Oil Oxidation Control by Active and Smart Packaging System Using Wheat Gluten/Chlorophyll Film to Increase Shelf Life and Detecting Expiration Date

AbstractIn this study, a smart biodegradable film based on wheat gluten modified with chlorophyll (WG/Ch) is presented. The effect of chlorophyll on the antioxidant activity and mechanical properties of WG/Ch film is studied. Scanning electron microscopy and Fourier‐transform infrared spectroscopy (FT‐IR) analyses are used to study the structure and chemical composition of gluten‐based films. The WG/Ch film is used in the packaging of sesame oil. The effect of WG/Ch film on increasing the shelf life and detecting expiration time of oil is studied. FT‐IR results show that weak interactions are created between gluten and chlorophyll. The antioxidant activity of the WG film is 60% and it increases to 85% by the addition of chlorophyll. The results show that with the increasing storage time of oil samples, oxidative indices are increased, but the WG/Ch film decreases the sesame oil oxidation significantly (almost in the all oxidative indices 50% decrease is observed).Practical Applications: The WG/Ch film is used in the packaging of sesame oil. The effect of WG/Ch film on the increasing of shelf life and detecting expiration time of oil is studied. By increasing storage time and oxidant, acidity, acid number, PA, and oil color change are increased, but the WG/Ch active film decreases the sesame oil oxidation significantly. Also, by examining the color properties of the active film, it is observed that with increasing storage time and oxidant percent, the color of the film is changed (from green to yellow), which can be used to estimate the oil expiration time. It should be mentioned that the film's color changes are visible to the naked eye.

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll.

Introduction: Photodynamic inactivation has been developed to kill pathogenic microbes. In addition, some techniques have been introduced to minimize the biofilm resistance to antifungal properties in inhibiting cell growth. The principle of photodynamic inactivation different to antifungal drugs therapy which is resistant to biofilms. The presence of reactive oxygen species (ROS) that generating in photodynamic inactivation mechanisms can be damaging of biofilm cells and the principle of light transmission that could be penetrating in matrix layers of extracellular polymeric substance (EPS) until reaching the target cells at the base layers of biofilm. The present work aims to explore the potential of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser. The potential of chlorophyll photosensitizer was evaluated based on the efficacy of inactivation C. albicans biofilm cell through a cell viability test and an organic compound test. Methods: The treatment of photoinactivation was administered to 12 groups of C. albicans biofilm for four days using the 445 nm laser and the 650 nm laser. The 445 nm and 650 nm lasers activated the chlorophyll extract of the papaya leaf (0.5 mg/L) at the same energy density. The energy density variation was determined as 5, 10, 20, 30 and 40 J/cm2 with the duration of exposure of each laser adjusted to the absorbance percentage of chlorophyll extract of the papaya leaf. Results: The absorbance percentage of chlorophyll extracts of the papaya leaf on wavelengths of 650 nm and 445 nm respectively were 22.26% and 60.29%, respectively. The most effective treated group was a group of the laser with the addition of chlorophyll, done by the 650 nm lasers with inactivation about 32% (P=0.001), while the 445 nm lasers only 25% (P=0.061). The maximum malondialdehyde levels by treatment of the laser 650 nm were (0.046±0.004) nmol/mg. Conclusion: The use of chlorophyll extract of the papaya leaf as a photosensitizer, resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation up to 32% (with chlorophyll) and 25% (without chlorophyll). The utilization of chlorophyll extract of the papaya leaf would increase the antifungal effects with the activation by the diode laser in the biofilm of C. albicans.

Effect of inlet air drying temperatures on the physicochemical properties and antioxidant activity of whey protein isolate-kale leaves chlorophyll (WPI-CH) microcapsules

The whey protein isolate-kale leaves chlorophyll (WPI-CH) microcapsules were prepared by spray drying in this work. Results from Fourier transform infrared spectra (FT-IR) showed that WPI-CH microcapsules were successfully obtained by forming the hydrogen bonds between WPI and chlorophyll, which resulted in shifting of the absorption band at 1074 cm−1 to 1078 cm−1 with a new absorption band revealed in 1049 cm−1. Effect of inlet air drying temperatures on the physicochemical properties and antioxidant activity of WPI-CH microcapsules were investigated. The result revealed that the moisture content of WPI-CH (20% addition) microcapsule was decreased by 21.1% with the inlet air drying temperature increased from 120 to 180 °C. Moreover, the encapsulation efficiency and solubility of chlorophyll were enhanced by 3.78% and 7.79%, respectively. Furthermore, DPPH scavenging capacity of WPI-CH microcapsules under different addition of chlorophyll were increased from 42.9% to 74.3%, 52.7%–82.7% and 71.8%–85.3%, respectively.

Cool-textured diets for use in baculovirus production

ABSTRACTThe incorporation of baculovirus into semi-synthetic diets has been used for the inoculation of large batches of insect larvae reared under gregarious conditions. The preparation of most artificial diets involves heating to dissolve the agar gelling agent followed by cooling to ∼55–60°C whereupon virus occlusion bodies (OBs) are incorporated into the diet. To avoid exposure of thermolabile OBs to high temperatures, a cool-textured diet using soy fiber was evaluated as a simple method to inoculate batches of larvae with the baculovirus OBs. Spodoptera exigua larvae that fed on a diet with 120, 140 or 160 g/l of soy fiber, as a substitute for agar, had a similar weight gain over a 24 h period than larvae that consumed a standard agar diet. The larval weight gain with cool-textured diets with 140 g/l of soy fiber was not improved by the addition of chlorophyll, green food dye colour, or by the removal of ascorbic acid, sodium benzoate and potassium sorbate. Preliminary tests performed to determine the optimal OBs concentration needed for the inoculation of groups of four instar larvae in agar and soy fiber diets revealed no virus inactivation in heated diets with a similar optimal concentration of 36 OBs mg−1 for both diets. Moreover, no significant differences were observed in OB production when each type of diet was used to inoculate groups of 250 larvae reared gregariously in plastic containers. Cool-textured diets could be of interest for the production of baculoviruses that require a diet that can be prepared quickly and easily.

들깨죽 제조를 위한 가열 중 클로로필과 카로텐이 지방질 산화와 토코페롤에 미치는 영향

야채가 혼입된 들깨죽 제조의 시뮬레이션 연구로 볶은 들깨와 쌀, 물의 혼합물의 가열 중 클로로필(0.33 mg/kg)과 베타-카로텐(3.3, 9.9, 19.8 mg/kg)의 첨가에 의한 지방질의 산화와 토코페롤의 변화를 모니터링하였다. 시료의 가열과정 중 과산화물값과 공액이중산값이 증가하여 들깨죽 지방질의 산화가 발생하였음을 뚜렷이 보여주었으며 클로로필과는 달리 베타-카로텐은 농도의존적으로 지방질의 과산화물값과 공액이중산값을 감소시켰다. 들깨와 쌀, 물의 혼합물에 첨가한 클로로필과 베타-카로텐 및 시료에 함유되어 있던 토코페롤의 분해는 가열 시간에 대해 1차반응이었으며 베타-카로텐은 가열 과정 중 토코페롤의 분해를 억제하였다. The effects of chlorophyll (0.33 mg/kg) and <TEX>${\beta}$</TEX>-carotene (3.3, 9.9, 19.8 mg/kg) addition to a mixture of roasted perilla seeds, rice, and water (30:45:225, w/w/w) on the lipid oxidation and tocopherol contents were studied during heating at <TEX>$100^{\circ}C$</TEX> for 120 min to simulate cooking of perilla and rice porridge. Lipid oxidation was evaluated with peroxide values (POV) and conjugated dienoic acid (CDA) values, and chlorophyll, <TEX>${\beta}$</TEX>-carotene, and tocopherols were determined by HPLC, POV, and CDA values were increased during heating, indicating the occurrence of lipid oxidation in the perilla and rice porridge. <TEX>${\beta}$</TEX>-Carotene decreased the POV and CDA values of the samples in a concentration-dependent manner, while the addition of chlorophyll did not affect them. Chlorophyll and <TEX>${\beta}$</TEX>-carotene which were added, and tocopherols naturally present in samples were degraded, following the first order kinetics during heating, and <TEX>${\beta}$</TEX>-carotene protected tocopherols from degradation.

Natural Chlorophyll but Not Chlorophyllin Prevents Heme-Induced Cytotoxic and Hyperproliferative Effects in Rat Colon

Diets high in red meat and low in green vegetables are associated with an increased risk of colon cancer. In rats, dietary heme, mimicking red meat, increases colonic cytotoxicity and proliferation of the colonocytes, whereas addition of chlorophyll from green vegetables inhibits these heme-induced effects. Chlorophyllin is a water-soluble hydrolysis product of chlorophyll that inhibits the toxicity of many planar aromatic compounds. The present study investigated whether chlorophyllins could inhibit the heme-induced luminal cytotoxicity and colonic hyperproliferation as natural chlorophyll does. Rats were fed a purified control diet, the control diet supplemented with heme, or a heme diet with 1.2 mmol/kg diet of chlorophyllin, copper chlorophyllin, or natural chlorophyll for 14 d (n = 8/group). The cytotoxicity of fecal water was determined with an erythrocyte bioassay and colonic epithelial cell proliferation was quantified in vivo by [methyl-(3)H]thymidine incorporation into newly synthesized DNA. Exfoliation of colonocytes was measured as the amount of rat DNA in feces using quantitative PCR analysis. Heme caused a >50-fold increase in the cytotoxicity of the fecal water, a nearly 100% increase in proliferation, and almost total inhibition of exfoliation of the colonocytes. Furthermore, the addition of heme increased TBARS in fecal water. Chlorophyll, but not the chlorophyllins, completely prevented these heme-induced effects. In conclusion, inhibition of the heme-induced colonic cytotoxicity and epithelial cell turnover is specific for natural chlorophyll and cannot be mimicked by water-soluble chlorophyllins.

클로로필의 광산화에 미치는 β-카로텐과 비타민 C의 영향

클로로필의 광산화에 미치는 β-카로텐과 비타민 C의 역할을 linoleic acid emulsion 모델 시스템과 피부조직 균질액을 이용하여 조사하고 이와 더불어 각각의 성분들의 조합시 일어나는 변화를 살펴보았다. Linoleic acid emulsion 모델 시스템에서 클로로필과 β-카로텐은 고농도에서 자외선 B에 의한 광산화를 촉진시키는 것으로 나타났으며 고농도의 비타민 C는 이를 억제시켰다. 갓김치에 함유되어 있는 클로로필, β-카로텐, 비타민 C 농도를 참고하여 이들을 조합하였을 때 자외선 B에 의한 광산화를 억제시켰으며 β-카로텐의 경우 단독으로 사용되었을 때에는 산화를 촉진하였으나 다른 항산화성분들과 함께 사용될 경우 항산화활성을 나타내었다. ICR mouse의 피부조직 균질액에 클로로필, β-카로텐, 비타민 C를 단독 또는 혼합하여 첨가하였을 때 클로로필 a, b 및 β-카로텐의 첨가는 광산화를 가속화시켰다. 비타민 C의 경우 50 ppm을 첨가하였을 때에는 아무런 효과를 나타내지 않았으나 500 ppm을 첨가하였을 때에는 자외선 B에 의한 광산화를 효과적으로 억제시켰다. 500 ppm의 비타민 C에 클로로필과 β-카로텐을 혼합하였을 때에도 광산화가 억제되었으며 비타민 C의 농도가 중요하게 작용하였다.

Chemical aspects of chlorophyll breakdown products and their relevance to canola oil stability

AbstractThe production of prooxidant compounds brought about through subjecting chlorophyll a or pheophytin a to laboratory‐scale processing in the presence of canola oil or tricapryloylglycerol was investigated. The addition of chlorophyll a (60 ppm) to canola oil prior to processing resulted in an oil of lowered stability. No large contribution to the produced instability by any one processing step was found when pheophytin a was added (60 ppm) to canola oil prior to processing. To isolate the effect of processing on the pigment, tricapryloylglycerol was used in the place of unsaturated canola oil as a carrier for pheophytin a (60 ppm). A control consisted of processed tricapryloylglycerol that had no added pheophytin prior to processing. The subsequent addition of pigment‐treated processed tricapryloylglycerol to linseed oil (1:1, w/w) caused a decrease in the stability of the latter, when compared with the control. No differences were observed between the prooxidant tricapryloylglycerol and the control tricapryloylglycerol by methods involving ultraviolet spectroscopy and thin‐layer or gas chromatography.

Antenna chlorophyll a has a structural role in stabilizing the functional conformation of P-700-chlorophyll-protein complexes

The heat-stability of P-700 photooxidation in Photosystem I particles from the thermophilic cyanobacterium Synechococcus elongatus dramatically decreased when bulk antenna chlorophyll a was extracted by diethyl ether treatment. The loss of the heat stability was related to the amount of extracted antenna chlorophyll a because the P-700 photooxidation became increasingly sensitive to heat as the chlorophyll a to P-700 ratio decreased. The heat-stability of P-700 photooxidation was substantially restored on addition of chlorophyll a , together with a lipid, to chlorophyll-depleted particles. Phosphatidylglycerol was most effective, followed by monogalactosyldiacylglycerol, whereas phosphatidylcholine, phosphatidylinositol and digalactosyldiacylglycerol had only minor effects. Addition of either chlorophyll a or lipid alone was without effect. A part of chlorophyll a rebound to the particles served as antenna of P-700 photooxidation and there was a good correlation between extents of the stabilization and increases in the antenna size. Thus, the stabilization of the P-700 photoresponse is ascribed to chlorophyll a molecules bound to the functional binding sites. The heat stability of P-700 photooxidation in Photosystem I particles from spinach was also largely lost by ether-treatment and restored by readdition of chlorophyll a and a lipid such as phosphatidylglycerol. It is concluded that antenna chlorophyll a has an important structural role in stabilizing P-700-chlorophyll-protein complexes.

Cooperative lengths and elastic constants in lipid bilayers: The chlorophyll a/dimyristoyllecithin system

In order to study the slow, cooperative motional modes induced by the addition of chlorophyll a (chl a) to dimyristoyllecithin (DML) bilayers, the T1, T1ρ, and T2 deuterium relaxation rates in 1,2-dimyristoyl(9,9′,10,10′-d4) phosphatidyl choline have been measured as a function of increasing chl a content. These data have been interpreted in terms of a model of fluctuating order directors. A fit of the T1ρ and T2 relaxation rates using this model reveal a trend of decreasing elastic constants and cooperative lengths as chl a is added.