Adaptive Optics Correction Research Articles

Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields.

Adaptive optics (AO) restore ideal imaging performance in complex samples by measuring and correcting optical aberrations, but often require custom-built microscopes with carefully aligned wavefront sensing/shaping devices and can be susceptible to sample motion. Here we describe NeAT, a computational framework using neural fields for AO two-photon fluorescence microscopy. NeAT estimates wavefront aberration and recovers sample structure from a 3D image stack without requiring external datasets for training. Incorporating motion correction in learning and correcting conjugation errors commonly found in commercial microscopes, NeAT is designed for deployment in biological laboratories for in vivo imaging. We validate NeAT's performance using a custom-built microscope with a wavefront sensor under varying signal-to-noise ratios, aberration, and motion conditions. With a commercial microscope, we demonstrate real-time aberration correction for in vivo morphological and functional imaging in the living mouse brain, with NeAT improving signal and accuracy of glutamate and calcium imaging of synapses and neurons.

Propagation of Sine hyperbolic Gaussian vortex beam (ShGvB) in vertical anisotropic oceanic turbulence with adaptive optics correction

The propagation of Sinh Gaussian vortex beam (ShGvB) along vertical anisotropic oceanic turbulence is established using the spatial power spectrum which depends upon the depth of the ocean. The average intensity of the beams has been derived. For 100 m depth, the average intensity has been plotted and studied using the measured salinity and temperature from the ocean in both isotropic and anisotropic oceanic turbulence systems. The average transmittance of the ShGvB is studied and found that the beam degrades faster in anisotropic media than in isotropic media. To mitigate the aberrations adaptive optics (AO) correction is incorporated in the study along the vertical link. Quantitative estimates of the Bit error rate (BER) have been made to evaluate the beam's performance for vertical underwater optical communication and found that by improving the signal-to-noise ratio and incorporating AO, the ShGvB performs effectively in vertical underwater optical communication.

Improvements on speed, stability and field of view in adaptive optics OCT for anterior retinal imaging using a pyramid wavefront sensor.

We present improvements on the adaptive optics (AO) correction method using a pyramid wavefront sensor (P-WFS) and introduce a novel approach for closed-loop focus shifting in retinal imaging. The method's efficacy is validated through in vivo adaptive optics optical coherence tomography (AO-OCT) imaging in both, healthy individuals and patients with diabetic retinopathy. In both study groups, a stable focusing on the anterior retinal layers is achieved. We further report on an improvement in AO loop speed that can be used to expand the imaging area of AO-OCT in the slow scanning direction, largely independent of the eye's isoplanatic patch. Our representative AO-OCT data reveal microstructural details of the neurosensory retina such as vessel walls and microglia cells that are visualized in single volume data and over an extended field of view. The excellent performance of the P-WFS based AO-OCT imaging in patients suggests good clinical applicability of this technology.

Sensorless adaptive optics in the second near-infrared window for deep vascular imaging in vivo.

We have experimentally validated the use of sensorless adaptive optics (AO) to enhance laser scanning confocal microscopy in the second near-infrared (NIR II) spectral range, termed as AO-NIR II confocal microscopy. This approach harnesses a NIR II fluorophore, excited by an 808 nm wavelength and emitting beyond 1000 nm, to visualize intricate structures in deep brain tissues with the intact skull. By leveraging the reduced scattering and aberrations in the NIR II spectrum, we successfully captured a three-dimensional (3D) vascular structure map extending 310 µm beneath the skull. AO typically boosts the fluorescence signal by approximately 2-3 times, leading to a superior contrast and diminished smearing effects. Consequently, small blood vessels at various depths can be clearly visualized, which might otherwise remain undetectable without AO corrections.

PASAT: pathfinder in solar adaptive telescope

In the forefront of quantitative solar physics research using large-aperture ground-based solar optical telescopes, high-contrast observation along with high-accuracy polarimetric measurement in the solar active region are required. In this paper, we propose a novel high-contrast imaging telescope construction with a 60 cm medium aperture, namely, the PAthfinder in Solar Adaptive Telescope (PASAT), in which a deformable secondary mirror is used as the adaptive optical correction device and a symmetrical optical path design is employed, leading to the least Muller matrix polarization instruments. The telescope can provide a high-resolution magnetic field with high accuracy for the solar active regions, as well as high-contrast images with a superior signal-to-noise ratio and photometric accuracy of the solar photosphere and chromosphere. These data will be directly used for a better understanding of the evolution and release of magnetic energy, which will help in improving space weather forecasting. Meanwhile, PASAT will accumulate the relevant techniques for constructing similar, larger solar telescopes in the future.

Pushing the limits of near-infrared photometry with the Gemini Multi-Conjugate Adaptive Optics System: study of crowded fields in the globular cluster M5

ABSTRACT We present the deepest J −Ks near-infrared photometry of the globular cluster M5 (NGC 5904) from observations taken with the Gemini South Adaptive Optics Imager in tandem with the Gemini Multi-conjugate adaptive optics System (GeMS) on the 8.1-m Gemini South telescope. Point spread function (PSF) photometry was carried out using a spatially variable PSF, zero-point calibrations based on correlations to a standard photometric catalogue, colour corrections, and crowding corrections. The latter corrections provided a new challenge given the field variations of the adaptive optics corrections in the central crowded regions of this cluster. The final photometric precision in our J− Ks colour–magnitude diagram exposes a dispersion among the lower main-sequence stars of M5 for the first time. This dispersion occurs below a main-sequence knee due to variations in the helium and CNO (carbon, nitrogen, and oxygen) abundances from multiple stellar populations, consistent with results from the bright evolved stars in this cluster from ultraviolet to near-infrared Hubble Space Telescope photometry and ground-based spectroscopy. This paper completes our original GeMS quality analysis programme, providing insights into adaptive optics analyses in crowded fields.

Daytime HyWFS approach for daylight adaptive optics wavefront sensing.

Bright daylight photon noise and the saturation of wavefront sensors pose challenges to high-resolution daytime imaging. In this paper, a daytime hybrid wavefront sensor (HyWFS) approach for real-time wavefront sensing in daylight adaptive optics (AO) is described. The Shack-Hartmann wavefront sensor (SHWFS) algorithm is used to efficiently compensate large-scale wavefronts, while the pyramid wavefront sensor (PyWFS) algorithm offers highly sensitive correction of small wavefronts. Daylight closed-loop AO experiments were performed using the daytime HyWFS approach with both algorithms, respectively. The experiment results indicate that the proposed approach provides accurate daylight AO correction and allows for a simple switch between the two algorithms without increasing system complexity. The daytime HyWFS approach can serve as an alternative for daylight natural guide star AO, enabling high-resolution observation of resident space objects no longer limited to dawn and dusk.

22 kW near-diffraction-limited Yb:YAG slab laser amplifier without adaptive optics correction

22 kW near-diffraction-limited Yb:YAG slab laser amplifier without adaptive optics correction

Poster Session II: Investigating photoreceptor function in disease-affected retinas using optoretinography.

Assessing the functional response of photoreceptors is vital in understanding retinal disease progression. Traditional subjective methods like visual acuity and visual fields, and objective ones like electroretinography, have limitations. An ideal complement to these techniques is optoretinography (ORG), which images the retina and tests its function at once. ORG utilizes the phase of the optical coherence tomography (OCT) signal to quantify nanometer-scale changes, measuring subtle photoreceptor responses to stimuli. Efforts to observe stimulus-evoked responses in human cone photoreceptors began with adaptive optics (AO) and common path interferometry, enabling the resolution and tracking of individual cells. Advances in OCT systems with cellular resolution through AO or digital aberration correction successfully measured ORG responses from single cones and rods. This method tracks phase differences between outer segment tips (COST or ROST) and the inner-outer segment junction (IS/OS) to assess individual cell responses. A novel velocity-based method recently demonstrated the feasibility of measuring ORG signals with clinical-grade OCT systems. In the present work, we implemented this technique on disease-affected human retinas, revealing lower magnitudes of response compared to healthy retinas, and highlighting its potential clinical applications.

Study on performance of a relay-assisted UWOC system based on adaptive optics

To improve the performance of underwater wireless optical communication (UWOC) systems, we propose a relay-assisted UWOC system model based on adaptive optics (AO). The closed expressions of the scintillation index, composite channel probability density function, and outage probability of the Gaussian beam before and after AO compensation are derived using the extended Rytov theory and Meijer G-function. The performance variation of an UWOC system with different parameters is analyzed by simulation. The results show that AO correction can compensate for the distorted wavefront and significantly reduce the intensity fluctuation at the receiving end. The proposed system can efficiently alleviate channel fading, improving the outage probability performance of the UWOC system.

Adaptive Optics Microscopy with Wavefront Sensing Based onNeighbor Correlation.

Complex structures in living cells and tissues induce wavefront errors when light waves pass through them, and images observed with optical microscopes are undesirably blurred. This problem is especially serious for living plant cells because images are strikingly degraded even within a single cell. Adaptive optics (AO) is expected to be a solution to this problem by correcting such wavefront errors, thus enabling high-resolution imaging. In particular, scene-based AO involves wavefront sensing based on the image correlation between subapertures in a Shack-Hartmann wavefront sensor and thus does not require an intense point light source. However, the complex 3D structures of living cells often cause low correlation between subimages, leading to loss of accuracy in wavefront sensing. This paper proposes a novel method for scene-based sensing using only image correlations between adjacent subapertures. The method can minimize changes between subimages to be correlated and thus prevent inaccuracy in phase estimation. Using an artificial test target mimicking the optical properties of a layer of living plant cells, an imaging performance with a Strehl ratio of approximately 0.5 was confirmed. Upon observation of chloroplast autofluorescence inside living leaf cells of the moss Physcomitrium patens, recovered resolution images were successfully obtained even with complex biological structures. Under bright-field illumination, the proposed method outperformed the conventional method, demonstrating the future potential of this method for label- and damage-free AO microscopy. Several points for improvement in terms of the effect of AO correction are discussed.

Optique adaptative : correction des effets de la turbulence atmosphérique sur les images astronomiques

Today, adaptive optics (AO) is installed on all very large astronomical telescopes. It allows to overcome the limitation in angular resolution imposed on large ground-based telescopes by the atmospheric turbulence perturbations. This review paper presents the state of the art of the field for astronomical applications. It gives the principles of AO and describes its main components which are the deformable mirrors, the wavefront sensors and the control algorithms. The paper also presents the main recent achievements and ongoing projects. First, for the direct imaging of extra-solar planets by extreme AO and coronography, taking as example the SPHERE instrument. Then to solve the problem of the very limited sky coverage by using laser guide star and to extend the AO correction beyond the isoplanatic patch. Thus, the various laser-assisted tomographic AO concepts are presented with examples of each realization as for the laser tomography AO, the multi-conjugate AO and the multi-object AO. Finally, the challenges of the new generation of giant telescopes are discussed at the end of the paper with a particular focus on the European Extremely Large Telescope (ELT) project.

Boosting 2-photon vision with adaptive optics.

The 2-photon effect in vision occurs when two photons of the same wavelength are absorbed by cone photopigment in the retina and create a visual sensation matching the appearance of light close to half their wavelength. This effect is especially salient for infrared light, where humans are mostly insensitive to 1-photon isomerizations and thus any perception is dominated by 2-photon isomerizations. This phenomenon can be made more readily visible using short-pulsed lasers, which increase the likelihood of 2-photon excitation by making photon arrivals at the retina more concentrated in time. Adaptive optics provides another avenue for enhancing the 2-photon effect by focusing light more tightly at the retina, thereby increasing the spatial concentration of incident photons. This article makes three contributions. First, we demonstrate through color-matching experiments that an adaptive optics correction can provide a 25-fold increase in the luminance of the 2-photon effect-a boost equivalent to reducing pulse width by 96%. Second, we provide image-based evidence that the 2-photon effect occurs at the photoreceptor level. Third, we use our results to compute the specifications for a system that could utilize 2-photon vision and adaptive optics to image and stimulate the retina using a single infrared wavelength and reach luminance levels comparable to conventional displays.

The Adaptive Optics System for the Gemini Infrared Multi-Object Spectrograph: Performance Modeling

The Gemini Infrared Multi-Object Spectrograph (GIRMOS) will be a near-infrared, multi-object, medium spectral resolution, integral field spectrograph (IFS) for Gemini North Telescope, designed to operate behind the future Gemini North Adaptive Optics system (GNAO). In addition to a first ground layer Adaptive Optics (AO) correction in closed loop carried out by GNAO, each of the four GIRMOS IFSs will independently perform additional multi-object AO correction in open loop, resulting in an improved image quality that is critical to achieve top level science requirements. We present the baseline parameters and simulated performance of GIRMOS obtained by modeling both the GNAO and GIRMOS AO systems. The image quality requirement for GIRMOS is that 57% of the energy of an unresolved point-spread function ensquared within a 0.1 × 0.1 arcsecond at 2.0 μ m. It was established that GIRMOS will be an order 16 × 16 adaptive optics (AO) system after examining the tradeoffs between performance, risks and costs. The ensquared energy requirement will be met in median atmospheric conditions at Maunakea at 30° from zenith.

Adaptive Optics Tip-Tilt Correction Based on Smith Predictor and Filter-Optimized Linear Active Disturbance Rejection Control Method.

A tip-tilt mirror (TTM) control method is designed to enhance the control bandwidth and ensure the rejection performance of the adaptive optics (AO) tip-tilt correction system. Optimized with the Smith predictor and filter, linear active disturbance rejection (LADRC) is adopted to achieve the tip-tilt correction. An AO tip-tilt correction experimental platform was built to validate the method. Experimental results show that the proposed method improves the control bandwidth of the system by at least 3.6 times compared with proportional-integral (PI) control. In addition, under the same control bandwidth condition, compared with the Smith predictor and proportional-integral (PI-Smith) control method, the system is more capable of rejecting internal and external disturbances, and its dynamic response performance is improved by more than 29%.

HAPS Based FSO Links Performance Analysis and Improvement With Adaptive Optics Correction

HAPS Based FSO Links Performance Analysis and Improvement With Adaptive Optics Correction

Enhanced neuroimaging with a calcium sensor in ex-vivo Drosophila melanogaster brains using closed-loop adaptive optics light-sheet fluorescence microscopy.

Adaptive optics (AO) has been implemented on several microscopy setups and has proven its ability to increase both signal and resolution. However, reported configurations are not suited for fast imaging of live samples or are based on an invasive or complex implementation method. Provide a fast aberration correction method with an easy to implement AO module compatible with light-sheet fluorescence microscopy (LSFM) for enhanced imaging of live samples. Development of an AO add-on module for LSFM based on direct wavefront sensing without requiring a guide star using an extended-scene Shack-Hartmann wavefront sensor. The enhanced setup uses a two-color sample labeling strategy to optimize the photon budget. Fast AO correction of in-depth aberrations in an ex-vivo adult Drosophila brain enables doubling the contrast when imaging with either cell reporters or calcium sensors for functional imaging. We quantify the gain in terms of image quality on different functional domains of sleep neurons in the Drosophila brain at various depths and discuss the optimization of key parameters driving AO. We developed a compact AO module that can be integrated into most of the reported light-sheet microscopy setups, provides significant improvement of image quality and is compatible with fast imaging requirements such as calcium imaging.

Analytical modelling of adaptive optics systems: Role of the influence function

Context. Adaptive optics (AO) is now a tool commonly deployed in astronomy. The real time correction of the atmospheric turbulence that AO enables allows telescopes to perform close to the diffraction limit at the core of their point spread function (PSF). Among other factors, AO-corrected PSFs depend on the ability of the wavefront corrector (WFC), generally a deformable mirror, to fit the incident wavefront corrugations. Aims. In this work, we focus on this error introduced by the WFC, the so-called fitting error. To date, analytical models only depend on the WFC cut-off frequency, and Monte Carlo simulations are the only solution for studying the impact of the WFC influence function shape on the AO-corrected PSF. We aim to develop an analytical model accounting for the influence function shape. Methods. We first obtain a general analytical model of the fitting error structure function. With additional hypotheses, we then derive an analytical model of the AO-corrected power spectral density. These two analytical solutions are compared with Monte Carlo simulations on different ideal profiles (piston, pyramid, Gaussian) as well as with real hardware (DM192 from ALPAO). Results. Our analytical predictions show a very good agreement with the Monte Carlo simulations. We show that in the image plane, the depth of the correction as well as the transition profile between the AO-corrected area and the remaining turbulent halo depend on the influence functions of the WFC. We also show that the generally assumed hypothesis of stationarity of the AO correction is actually not met. Conclusions. As the fitting error is the intrinsic optimal limit of an AO system, our analytical model allows for the assessment of the theoretical limits of extreme AO systems limited by the WFC in high-contrast imaging through a context where other errors become comparable.

Fiber coupling efficiency in ocean with adaptive optics corrections

Underwater optical wireless communication (UOWC) is a very promising technology that enables high-speed data transfer through the use of laser beams in an oceanic turbulent medium. The high-tech fiber optical devices, which are already available in the market, can be integrated with the UOWC systems. When integration is achieved, oceanic turbulence, which distorts the wavefront of the propagating laser beam, plays an important role in reducing the fiber coupling efficiency (FCE), which in turn results in reducing the light power received from the fiber optical components. In this paper, we propose the use of the adaptive optics technique in a UOWC system to mitigate the effects of oceanic turbulence and boost the FCE. For this reason, the field correlation for a Gaussian laser beam is derived by using the Huygens–Fresnel principle. This way, the light power over the coupling lens and the light power accepted by the fiber core are formulated under the effect of adaptive optics corrections, which are represented by the number of Zernike modes. The results demonstrate that under the oceanic turbulence effect, the FCE of the UOWC system employing adaptive optics is always larger than that of the UOWC system employing no adaptive optics.

Volumetric light sheet imaging with adaptive optics correction.

Light sheet microscopy has developed quickly over the past decades and become a popular method for imaging live model organisms and other thick biological tissues. For rapid volumetric imaging, an electrically tunable lens can be used to rapidly change the imaging plane in the sample. For larger fields of view and higher NA objectives, the electrically tunable lens introduces aberrations in the system, particularly away from the nominal focus and off-axis. Here, we describe a system that employs an electrically tunable lens and adaptive optics to image over a volume of 499 × 499 × 192 μm3 with close to diffraction-limited resolution. Compared to the system without adaptive optics, the performance shows an increase in signal to background ratio by a factor of 3.5. While the system currently requires 7s/volume, it should be straightforward to increase the imaging speed to under 1s per volume.