Acylated Ghrelin Concentrations Research Articles

Acute effects of exercise intensity on butyrylcholinesterase and ghrelin in young men: A randomized controlled study

Background/objectivesButyrylcholinesterase (BChE), a liver-derived enzyme that hydrolyzes acylated ghrelin to des-acylated ghrelin, may trigger a potential mechanism responsible for the acute exercise-induced suppression of acylated ghrelin. However, studies examining the effects of an acute bout of high-intensity exercise on BChE and acylated ghrelin have yielded inconsistent findings. This study aimed to examine the acute effects of exercise intensity on BChE, acylated ghrelin and des-acylated ghrelin concentrations in humans. MethodsFifteen young men (aged 22.7 ± 1.8 years, mean ± standard deviation) completed three, half-day laboratory-based trials (i.e., high-intensity exercise, low-intensity exercise and control), in a random order. In the exercise trials, the participants ran for 60 min (from 09:30 to 10:30) at a speed eliciting 70 % (high-intensity) or 40 % (low-intensity) of their maximum oxygen uptake and then rested for 90 min. In the control trial, participants sat on a chair for the entire trial (from 09:30 to 12:00). Venous blood samples were collected at 09:30, 10:00, 10:30, 11:00, 11:30 and 12:00. ResultsThe BChE concentration was not altered over time among the three trials. Total acylated and des-acylated ghrelin area under the curve during the first 60 min (i.e., from 0 min to 60 min) of the main trial were lower in the high-intensity exercise trial than in the control (acylated ghrelin, mean difference: 62.6 pg/mL, p < 0.001; des-acylated ghrelin, mean difference: 31.4 pg/mL, p = 0.035) and the low-intensity exercise trial (acylated ghrelin, mean difference: 87.7 pg/mL, p < 0.001; des-acylated ghrelin, mean difference: 43.0 pg/mL, p = 0.042). ConclusionThe findings suggest that BChE may not be involved in the modulation of ghrelin even though lowered acylated ghrelin concentration was observed after high-intensity exercise.

Plasma Concentrations of Gut Hormones Acyl Ghrelin and Peptide YY and Subsequent Risk of Colorectal Cancer and Molecular Tumor Subtypes.

The findings of this study do not support a major role for the metabolic gut hormones ghrelin and PYY in colorectal cancer development but suggest the possibility of an involvement for ghrelin in specific tumor subtypes. Elucidating subtype-specific risk factors and mechanisms of carcinogenesis may have implications for precision prevention.

Objective and Subjective Appetite Assessment in Patients with Gynecological Cancer: A Pre- and Post-Operative Pilot Study.

Although appetite and its disorders have been implicated in disease progression and outcomes, ghrelin concentrations, an objective appetite measure, are rarely assessed in patients with gynecological malignancies. The present study aimed to assess changes in post-operative versus pre-operative appetite levels in patients with gynecological cancers scheduled for tumor removal surgery (N = 53). Acylated ghrelin concentrations were assessed as an objective appetite proxy, whereas the Council of Nutrition appetite questionnaire (CNAQ) was employed as a subjective appetite measure. Ghrelin concentrations were increased post-operatively (median: 12.1 pg/mL, IQR: 0.67 to 23.5, p-value = 0.001) but the perceived appetite of patients (CNAQ) remained unchanged (median: −1, IQR: −3 to 1). Tumor removal surgery decreased all anthropometric indices (body weight, body mass index, waist and hips circumferences, triceps skinfolds, body fat, fat mass and fat mass index, p-value ≤ 0.001 for all) and doubled the risk of malnutrition among patients. No difference was recorded in the change in participants’ objective and subjective appetite when they were classified according to the tumor type. No correlation was observed between ghrelin concentrations and CNAQ score pre-operatively (Spearman’s rho correlation coefficient = −0.181, p-value = 0.298) or post-operatively (Spearman’s rho correlation coefficient = 0.071, p-value = 0.684). The observed post-operative rise in ghrelin concentrations is associated with body weight loss and consists of a possible defense mechanism of the human body, aiming to prolong survival.

Dietary Pharmacological Zinc and Copper Enhances Voluntary Feed Intake of Nursery Pigs

The objective of the three experiments herein were to characterize the effect of pharmacological zinc and copper concentrations on nursery pig feed intake, stomach ghrelin, energy and nutrient digestibility, and mineral retention in post-weaned pigs. In Expt. 1, 300 weaned pigs were allotted across three dietary treatments (n = 10 pens/treatment) and fed in two diet phases (P1 and P2) lasting 7 and 14 days, respectively. Treatments were: (1) Control diet with no pharmacological minerals in P1 and P2, CON; (2) CON + 3,000 mg/kg Zn and 200 mg/kg Cu (P1), no pharmacological minerals in P2, ZC-CON; and (3) CON + 3,000 mg/kg Zn and 200 mg/kg Cu (P1), CON + 2,000 mg/kg Zn and 200 mg/kg Cu (P2); ZC. Over the 21-day test period, ZC pigs had 15% higher ADG and 13–24% ADFI compared to the CON and ZC-CON pigs (P &amp;lt; 0.05). ZC-CON and ZC pig daily feed intakes were 29 and 73% higher by day 5 and 7 post-weaning, respectively, compared to the CON pigs (P &amp;lt; 0.0001). However, removing pharmacological minerals in P2 abruptly decreased ZC-CON daily feed intake within 24 h to similar intakes as the CON compared to the ZC pigs (0.17, 0.14, and 0.22 kg/d, respectively, P &amp;lt; 0.05). Dietary pharmacological minerals increased stomach fundus ghrelin-positive cells than CON pigs at day 7 (P = 0.005) and day 21 (P &amp;lt; 0.001). However, fasting plasma total and acyl-ghrelin concentrations did not differ from a control in response to zinc oxide daily drenching (Expt. 2). Expt. 3 showed that zinc and copper to have moderate to low retention; however, pharmacological zinc and copper diets increased zinc (P &amp;lt; 0.05) and copper retention (P = 0.06) after 28 days post-weaning compared to control pigs. Pharmacological zinc and copper did not improve digestible energy, metabolizable energy or nitrogen balance. Altogether, dietary pharmacological zinc and copper concentrations improve growth rates and mineral retention in nursery pigs. This improved performance may partially be explained by increased stomach ghrelin abundance and enhanced early feed intake in newly weaned pigs fed pharmacological concentrations of zinc and copper.

Energetic demands of lactation produce an increase in the expression of growth hormone secretagogue receptor in the hypothalamus and ventral tegmental area of the rat despite a reduction in circulating ghrelin.

Lactating rats show changes in the secretion of hormones and brain signals that promote hyperphagia and facilitate the production of milk. Little is known, however, about the role of ghrelin in the mechanisms sustaining lactational hyperphagia. Here, we used Wistar female rats that underwent surgery to sever the galactophores to prevent milk delivery (GC rats) and decrease the energetic drain of milk delivery. We compared plasma acyl-ghrelin concentrations and growth hormone secretagogue receptor (GHSR) mRNA expression in different brain regions of GC rats with those of sham operated lactating and nonlactating rats. Additional lactating and nonlactating rats were implanted with cannulae aimed at the lateral ventricles and were used to compare feeding responses to central ghrelin or GHSR antagonist infusions to those of nonlactating rats receiving similar infusions on day 14-16 postpartum (pp). Results show lower plasma acyl-ghrelin concentrations on day 15 pp sham operated lactating rats compared to GC or nonlactating rats. These changes occur in association with increased GHSR mRNA expression in the hypothalamic arcuate nucleus (ARC) and ventral tegmental area (VTA) of sham operated lactating rats. Despite lactational hyperphagia, infusions of ghrelin (0.25 or 1μg) resulted in similar increases in food intake in lactating and nonlactating rats. In addition, infusions of the GHSR antagonist JMV3002 (4μg in 1μl of vehicle) produced greater suppression of food intake in lactating rats than in nonlactating rats. These data suggest that, despite lower plasma ghrelin, the energetic drain of lactation increases sensitivity to the orexigenic effects of ghrelin in brain regions important for food intake and energy balance, and these events are associated with lactational hyperphagia.

Blood Acylated Ghrelin Concentrations in Healthy Term Newborns: A Prospective Cohort Study

Objective The effect of ghrelin, a growth hormone (GH) secretagogue on growth of neonates, has been studied in the past, but not fully clarified. We aimed to investigate the relationship between ghrelin and growth parameters at birth and at the age of three months in healthy term infants. Methodology. This was a prospective observational study carried out in a tertiary care hospital. Eighty-four infants born at gestational ages between 37 and 42 weeks and classified as term small for gestational age (SGA) and appropriate for gestational age (AGA) were included in the study. Estimation of acylated ghrelin (AG) concentrations was done in the cord blood at birth and in venous blood at the age of 3 months in all the infants. The correlation between AG concentrations and growth parameters at birth and at 3 months was studied. Results AG concentrations were significantly higher in SGA (236.16 ± 152.4 pg/ml) than AGA neonates (59.45 ± 20.95 pg/ml) at birth. Concentrations were observed to be negatively correlated with birth weight (r = −0.34, p value 0.03), birth length, and head circumference (r = −0.509 and -0.376, respectively) in SGA neonates. However, at 3 months, AG concentrations did not correlate with changes in anthropometric parameters in both the groups. Conclusion Cord acylated ghrelin concentrations are higher in SGA neonates, and the concentrations are inversely proportional to the birth weight. Hence, its role as a surrogate marker for intrauterine nutrition can be suggested. However, its concentrations do not correlate with anthropometric parameters in early postnatal growth, suggesting it may not have a direct role in postnatal growth.

Circulating acyl and des-acyl ghrelin levels in obese adults: a systematic review and meta-analysis

Ghrelin is the only known orexigenic gut hormone, and its synthesis, secretion and degradation are affected by different metabolic statuses. This meta-analysis aimed to investigate the potential differences in plasma acyl ghrelin (AG) and des-acyl ghrelin (DAG) concentrations between normal weight and obese adults. Systematic literature searches of PubMed, Embase and Web of Science through October 2021 were conducted for articles reporting AG or DAG levels in obesity and normal weight, and 34 studies with 1863 participants who met the eligibility criteria were identified. Standardized mean differences (SMDs) with 95% confidence intervals (CIs) were calculated to evaluate group differences in circulating AG and DAG levels. Pooled effect size showed significantly lower levels of baseline AG (SMD: − 0.85; 95% CI: − 1.13 to − 0.57; PSMD < 0.001) and DAG (SMD: − 1.06; 95% CI: − 1.43 to − 0.69; PSMD < 0.001) in obese groups compared with healthy controls, and similar results were observed when subgroup analyses were stratified by the assay technique or storage procedure. Postprandial AG levels in obese subjects were significantly lower than those in controls when stratified by different time points (SMD 30 min: − 0.85, 95% CI: − 1.18 to − 0.53, PSMD < 0.001; SMD 60 min: − 1.00, 95% CI: − 1.37 to − 0.63, PSMD < 0.001; SMD 120 min: − 1.21, 95% CI: − 1.59 to − 0.83, PSMD < 0.001). In healthy subjects, a postprandial decline in AG was observed at 120 min (SMD: − 0.42; 95% CI: − 0.77 to − 0.06; PSMD = 0.021) but not in obese subjects (SMD: − 0.28; 95% CI: − 0.60 to 0.03; PSMD = 0.074). The mean change in AG concentration was similar in both the obese and lean health groups at each time point (ΔSMD30min: 0.31, 95% CI: − 0.35 to 0.97, PSMD = 0.359; ΔSMD60min: 0.17, 95% CI: − 0.12 to 0.46, PSMD = 0.246; ΔSMD120min: 0.21, 95% CI: − 0.13 to 0.54, PSMD = 0.224). This meta-analysis strengthens the clinical evidence supporting the following: lower baseline levels of circulating AG and DAG in obese individuals; declines in postprandial circulating AG levels, both for the healthy and obese individuals; a shorter duration of AG suppression in obese subjects after meal intake. These conclusions have significance for follow-up studies to elucidate the role of various ghrelin forms in energy homeostasis.

Effects of a 12-Week Diet versus Diet plus Aerobic and Resistance Exercise Program on Acylated and Desacylated Ghrelin, and Ghrelin O-Acyltransferase in Adolescent Girls with Obesity.

This study investigated the effects of a 12-week diet versus diet plus aerobic and resistance exercise programme on acylated ghrelin (AG), desacylated ghrelin (DAG), and ghrelin O-acyltransferase (GOAT) concentrations in girls with obesity. We randomised 30 adolescents with obesity to a 12-week aerobic and resistance exercise group (EG) or a control group (CG). At baseline and at 4, 8, and 12 weeks, we measured their body composition, lipid profile, glucose, AG, DAG, and GOAT concentrations. In the EG, the body fat percentage decreased by 2.37% and was significantly lower than that in the CG. The DAG concentrations significantly increased by 48.3% and 27.4% in the EG and CG, respectively. At 4, 8, and 12 weeks, DAG concentrations were significantly higher in the EG than in the CG. AG concentrations were higher at week 12 than at baseline in both groups. In both groups, the GOAT concentrations increased at weeks 8 and 12; however, no between-group differences were observed in the changes in GOAT concentrations. This study showed increased DAG concentrations and non-significant changes in AG and GOAT concentrations after a 12-week aerobic and resistance exercise programme in girls with obesity. These findings suggest that an aerobic and resistance exercise programme influences appetite-regulating hormones, mainly through changes in DAG concentrations.

Does butyrylcholinesterase mediate exercise-induced and meal-induced suppression in acylated ghrelin?

Ample evidence supports the notion that an acute bout of aerobic exercise and meal consumption reduces acylated ghrelin concentration. However, the mechanisms by which this exercise- and meal-induced suppression of acylated ghrelin occurs in humans is unknown. This study aimed to examine the concentration of butyrylcholinesterase (BChE), an enzyme responsible for hydrolysing ghrelin and other appetite-related hormones in response to a single bout of running and a standardised meal in young, healthy men. Thirty-three men (aged 23 ± 2 years, mean ± standard deviation) underwent two (exercise and meal conditions) 2-h laboratory-based experiments. In the exercise condition, all participants ran for 30 min at 70% of the maximum oxygen uptake (0930-1000) and rested until 1130. In the meal condition, participants reported to the laboratory at 0930 and rested until 1000. Subsequently, they consumed a standardised meal (1000-1015) and rested until 1130. Blood samples were collected at baseline (0930), 1000, 1030, 1100 and 1130. BChE concentration was not altered in both the exercise and meal conditions (p > 0.05). However, acylated ghrelin was suppressed after exercise (p < 0.05) and meal consumption (p < 0.05). There was no association between the change in BChE concentration and the change in acylated ghrelin before and after exercise (p = 0.571). Although des-acylated ghrelin concentration did not change during exercise (p > 0.05), it decreased after meal consumption (p < 0.05). These findings suggest that BChE may not be involved in the suppression of acylated ghrelin after exercise and meal consumption.

Effects of Aerobic Exercise on Appetite and Plasma Acylated Ghrelin Concentrations in Athletes

Effects of Aerobic Exercise on Appetite and Plasma Acylated Ghrelin Concentrations in Athletes

Age, gestational and heat stress effects on ghrelin secretion in dairy cattle

It is known that heat stress decreases dry matter intake in cattle with impacts on milk production and fertility. Ghrelin is an orexigenic hormone with suppressive effects on reproduction. In this study, we investigated the effects of heat stress and gestational status on ghrelin secretion and its possible associations with DMI in Holstein cattle. The study was conducted in a dairy farm without any artificial cooling measures. The animals were fed a total mixed ration twice daily; each morning the leftovers were removed and weighted. Lactating cows and heifers were used during the winter and the summer; in each season 8 groups were formed as following: non-pregnant cows (n = 10) and non-pregnant heifers (n = 10) and pregnant cows (3 groups, each n = 8) and heifers (3 groups, each n = 10), being at the 1st (days 65–90), the 2nd (days 114–144) and the 3rd (dry cows, days 198–220; heifers, days 192–230) trimester of gestation. In each season the blood samples were collected from all groups on the same day, 1 h prior to morning feeding. In the winter, the Temperature Humidity Index (THI) was 58 in the winter and 73 in the summer. Normal and acidified sera were stored at −20 °C and analyzed for cortisol, total and acylated ghrelin concentrations, respectively. T-Test and Welch-Satterthwaite were performed for continuous data comparison, while two-way ANOVA to test for differences between gestation and season. Feed refusals were higher (p < 0.01) during the summer compared to the winter. In cows, total ghrelin levels differed between gestation stages in winter and summer(p < 0.04), while acylated ghrelin levels differed by gestation stage in winter (p < 0.001) but not in summer. There was an effect of season by the gestational stage in the pattern of acylated (p < 0.001) but not of total ghrelin. In heifers, the pattern of total and acylated ghrelin secretion was not affected by season or gestation stage (p > 0.05). Both in cows and heifers, acylated ghrelin levels were lower in summer compared to winter, (p < 0.002). During the summer months the low ghrelin levels might explain the reduced feed consumption of heat stressed animals. We infer that the lactation-induced altered metabolic status of the animals governed the different ghrelin levels at various gestational stages in cows and heifers.

Measurement of serial serum total and acylated ghrelin levels in critically ill patients: A prospective and observational pilot study.

Ghrelin is a hormone that regulates appetite and energy metabolism. The change of serial serum total and acylated ghrelin levels during hospital stays of critical patients are unknown. In addition, the relationship of this change with the clinical results of patients in the intensive care unit (ICU) is also unknown. The aim of this study was to determine serum total and acylated ghrelin levels serially in critically ill patients. This prospective study was performed in the ICU. Patients who were >18 years old and stayed in ICU for >48 h were included in the study. Serum total and acylated ghrelin concentrations were measured at baseline in all participants and serially on the 2nd, 5th, and 10th day after entry into the study in those who remained in the ICU. A total of 60 participants were included. The mean age was 56 ± 21 years. (Baseline, 2nd, 5th, and 10th day median serum total ghrelin levels were 3551 (1651-3995), 3485.20 (1379-4071), 3359 (1167-3919), and 3355 pg/ml (2207-3843), respectively. Baseline, 2nd, 5th, and 10th day acylated ghrelin levels were 47 (0-673), 50 (0-730), 73 (0-808), and 125 pg/ml (0-689), respectively. There was no significant difference between total ghrelin/acylated ghrelin levels and mortality (P > .05). ICU mortality was 30%. Ghrelin levels were decreased slightly and acylated ghrelin levels increased substantially over time in critically ill patients. There were no differences between serum total ghrelin/acylated ghrelin levels and ICU mortality .

Effects of moderate to vigorous intensity cycling on appetite, ad libitum energy intake and appetite-related hormones in healthy South Asian and white European men

Compensatory changes in appetite and energy intake do not appear to occur in the short-term after acute exercise; however, responses have not been compared in South Asians, a group at high risk of central obesity and type 2 diabetes, with white Europeans. This study examined appetite perceptions, energy intake and appetite-related hormones after moderate-to-vigorous intensity cycling in South Asian versus white European men. Fifteen South Asians (mean(SD) 29(8) years; 25.4(4.5) kg m−2) and fifteen white Europeans (33(10) years; 26.1(3.8) kg m−2) matched for age and body mass index completed two 7 h trials (control and exercise). Participants rested throughout both trials apart from completing 60 min cycling at 2–3 h in the exercise trial. A standardised breakfast was consumed at 0 h and an ad libitum buffet meal at 4 h. Appetite perceptions and appetite-related hormones were measured at predetermined intervals. Exercise suppressed acylated ghrelin (d = 0.19, P < 0.001) and increased total peptide YY (PYY) (d = 0.14, P = 0.004), insulin (d = 0.09, P = 0.046) and glucose concentrations (d = 0.31, P < 0.001) (main effect of trial), without stimulating compensatory increases in energy intakes in either group (group-by-trial interactions). South Asians exhibited lower absolute energy intake and higher insulin concentrations than white Europeans (main effect group d ≥ 0.63, P ≤ 0.003), whereas group-by-time interactions revealed lower acylated ghrelin concentrations at 3 and 4 h (d ≥ 0.75, P ≤ 0.038) and higher glucose concentrations at 0.75 and 2 h (d ≥ 0.67, P ≤ 0.008) in South Asian than white European men. These findings demonstrate that acute exercise induces a short-term energy deficit and similar appetite responses in South Asian and white European men.

Acute ketosis inhibits appetite and decreases plasma concentrations of acyl ghrelin in healthy young men.

To investigate the acute effect of ketone ester (KE) ingestion on appetite and plasma concentrations of acyl ghrelin (AG), unacylated ghrelin (UAG) and glucagon-like peptide-1 (GLP-1) secretion, and to compare responses with those elicited by isocaloric glucose (GLU) administration. We examined 10 healthy young men on three separate occasions using a placebo (PBO)-controlled crossover design. A KE versus taste-matched isovolumetric and isocaloric 50% GLU and taste-matched isovolumetric PBO vehicle was orally administered. Our main outcome measures were plasma concentrations of AG, UAG, glucose-dependent insulinotropic polypeptide (GIP) and GLP-1 along with appetite sensation scores assessed by visual analogue scale. KE ingestion resulted in an average peak beta-hydroxybutyrate concentration of 5.5 mM. AG and UAG were lowered by approximately 25% following both KE and GLU intake compared with PBO. In the case of AG, the differences were -52.1 (-79.4, -24.8) for KE and -48.4 (-75.4, -21.5) pg/mL for GLU intake (P < .01). Concentrations of AG remained lower with KE but returned to baseline and were comparable with PBO levels after GLU intake. GLP-1, GIP, gastrin and cholecystokinin were not affected by KE ingestion. Our results suggest that the suppressive effects on appetite sensation scores associated with hyperketonaemia are more probable to be mediated through reduced ghrelin concentrations than by increased activity of cholecystokinin, gastrin, GIP or GLP-1.

Effects of energy balance on appetite and physiological mediators of appetite during strenuous physical activity: secondary analysis of a randomised crossover trial.

Energy deficit is common during prolonged periods of strenuous physical activity and limited sleep, but the extent to which appetite suppression contributes is unclear. The aim of this randomised crossover study was to determine the effects of energy balance on appetite and physiological mediators of appetite during a 72-h period of high physical activity energy expenditure (about 9·6 MJ/d (2300 kcal/d)) and limited sleep designed to simulate military operations (SUSOPS). Ten men consumed an energy-balanced diet while sedentary for 1 d (REST) followed by energy-balanced (BAL) and energy-deficient (DEF) controlled diets during SUSOPS. Appetite ratings, gastric emptying time (GET) and appetite-mediating hormone concentrations were measured. Energy balance was positive during BAL (18 (sd 20) %) and negative during DEF (-43 (sd 9) %). Relative to REST, hunger, desire to eat and prospective consumption ratings were all higher during DEF (26 (sd 40) %, 56 (sd 71) %, 28 (sd 34) %, respectively) and lower during BAL (-55 (sd 25) %, -52 (sd 27) %, -54 (sd 21) %, respectively; Pcondition < 0·05). Fullness ratings did not differ from REST during DEF, but were 65 (sd 61) % higher during BAL (Pcondition < 0·05). Regression analyses predicted hunger and prospective consumption would be reduced and fullness increased if energy balance was maintained during SUSOPS, and energy deficits of ≥25 % would be required to elicit increases in appetite. Between-condition differences in GET and appetite-mediating hormones identified slowed gastric emptying, increased anorexigenic hormone concentrations and decreased fasting acylated ghrelin concentrations as potential mechanisms of appetite suppression. Findings suggest that physiological responses that suppress appetite may deter energy balance from being achieved during prolonged periods of strenuous activity and limited sleep.

The association of plasma acyl ghrelin level with alcohol craving in early abstinent alcohol dependent patients

Background:Craving plays an important role in maintenance of alcohol dependence. Earlier studies have analyzed the role of ghrelin in craving and their results have been heterogenous. Acyl ghrelin is its more active form as it crosses the blood brain barrier. Hence we aimed to examine the relationship between plasma acyl ghrelin and craving in Indian patients having alcohol dependence syndrome.Methods:The present study was a hospital-based prospective study. A total of 60 drug-naive patients of alcohol dependence and 30 healthy controls were included. After taking informed consent fasting blood samples were collected from them on day 1 and tested for plasma acyl ghrelin level. Fasting blood samples were repeated in all cases on day 14. During this time, we also assessed the patients' cravings by obsessive compulsive drinking scale, and alcohol craving questionnaire; and withdrawal by clinical institute withdrawal assessment for alcohol scale. These scales were repeated on day 14. Data analysis was done by SPSS version 25.0.Results:Plasma concentrations of acyl ghrelin increased significantly during early abstinence in patients from day 1 to day 14 (P < 0.0001). Pearson correlation test revealed a trend of positive correlation between plasma concentration of acyl ghrelin on day 14 and severity of craving on day 1.Conclusion:Our results suggest the plasma concentration of acyl ghrelin may be a predictor of severity of alcohol craving during early abstinence. Anti-craving drugs acting on acyl ghrelin level in brain may open an innovative avenue for optimum treatment of alcohol dependence.

Comparative study of acylated ghrelin levels in obese diabetes mellitus type 2 and lean diabetes mellitus type 2 female aged 30-45.

Objectives: To critically analyze the concentration of Acylated ghrelin and its comparative level between lean and obese type two Diabetes Mellitus female patients aged Thrity-fourty five. Study Design: Cohort study design. Setting: Department of Endocrinology, Lahore General Hospital, Lahore and Sheikh Zayad Hospital Lahore. Period: 1st March 2019 to 31st August 2019 (6 months). Material &amp; Methods: Blood sample of fifty female individuals were taken after twelve hours of fasting which were divided into two groups obese type two diabetes mellitus patients having BMI&gt; twenty eight and lean type two diabetes mellitus patients. For measurement of ghrelin concentrations, blood samples collected in specialized EDTA–aprotinin chilled test tubes were immediately centrifuged at two hundreds rpm and their acidification with 1 mol/L HCl (10% of sample volume) done to adjust pH four to preserve plasma acylated ghrelin. The serum was stored at -20c for hormonal assay of Ghrelin by Elisa kit of Biovender Laboratani of Czek Republic. Result: The statistical analysis of Acylated ghrelin concentration was done by using paired t-test and one way ANOVA showing significant difference between the concentration of ghrelin in both obese and lean with reference to BMI having P-value&lt;0.0001. Conclusions: The study showed that acylated ghrelin levels were comparative on the lower side in obese type two DM as compared to lean type two DM with significant P-value &lt;0.0001 by using two-tailed P-value.

Dietary macronutrient regulation of acyl and desacyl ghrelin concentrations in children with Prader-Willi syndrome (PWS).

The effects of dietary macronutrients on orexigenic and anorexigenic hormones in children are poorly understood. To explore effects of varying dietary macronutrients on appetite-regulating hormones [acyl ghrelin (AG) and desacyl ghrelin (DAG), glucagon-like peptide 1 (GLP-1), peptide tyrosine tyrosine (PYY) and insulin] in children with PWS and healthy children (HC). Randomized, cross-over experiments compared two test diets [high protein-low carbohydrate (HP-LC) and high protein-low fat (HP-LF)] to a STANDARD meal (55% carbohydrate, 30% fat, 15% protein). Experiment 1 included ten children with PWS (median age 6.63years; BMI z 1.05); experiment 2 had seven HC (median age 12.54years; BMI z 0.95). Blood samples were collected at baseline and at 60-minute intervals for 4hours. Independent linear mixed models were adjusted for age, sex and BMI z-score. Fasting and post-prandial AG and DAG concentrations are elevated in PWS children; the ratio of AG/DAG is normal. Food consumption reduced AG and DAG concentrations in both PWS and HC. GLP-1 levels were higher in PWS after the HP-LC and HP-LF meals than the STANDARD meal (P=.02-0.04). The fasting proinsulin to insulin ratio (0.08 vs 0.05) was higher in children with PWS (P=.05) than in HC. Average appetite scores in HC declined after all three meals (P=.02) but were lower after the HP-LC and HP-LF meals than the STANDARD meal. Altered processing of proinsulin and increased GLP-1 secretion in children with PWS after a high protein meal intake might enhance satiety and reduce energy intake.

The Relationship Between Sensory Attributes of Beverages and Change in Acylated Ghrelin Concentrations

Abstract Objectives To investigate sensory attributes of carbonated, flavored, and sweetened beverage consumption, and relationship to the hunger hormone, acylated ghrelin. Methods In a randomized single-blinded crossover design, males (n = 14) and females (n = 15); aged 23–65; BMI &amp;lt; 30 kg/m2; with no reported chronic disease consumed six different beverages (water, carbonated no flavor [CNF], carbonated lime flavor [CL], degassed lime flavor [DL], carbonated lime flavor with aspartame [CLS], and degassed lime flavor with aspartame [DLS]) at separate time points. Participants were asked to consume a breakfast meal (approximately 400 kcals) and then fast for approximately 4 hours. Blood was collected via finger-stick at baseline; and 10 and 45 minutes after consumption of the beverage. ELISA was used to determine acylated ghrelin concentration. A 7-pt likert scale was used to determine acceptability of the treatment beverages. ANOVA and spearman rank-order correlation coefficients were used to determine differences in liking of sensory attributes between beverages and correlations between variables, respectively. Results The CNF, CL, and water beverages were well liked by participants with mean overall liking scores of greater than 5. The overall liking scores were not significantly different between these three beverages (P &amp;gt; 0.05). There were significant differences between CNF, CL, and water and the remaining 3 beverages – DL, CLS, and DLS. Degassed and sweetened beverages (DL, CLS, and DLS) were least liked with mean liking scores ranging from 3.69–4.28. There were no significant correlations (P &amp;gt; 0.05) found between change in acylated ghrelin concentration and overall liking or liking of beverage attributes. Conclusions Acceptability of sensory attributes, including carbonation, flavor, and sweetness, was not related to change in acylated ghrelin concentrations after the consumption of six different beverages. Funding Sources This work was funded by the College of Saint Benedict/Saint John's University Faculty Research/Development Grant.

Comparing Hunger and Thirst Perception and Related Biomarker Response After Carbonated Beverage Consumption

Abstract Objectives To determine if there were relationships between biomarkers of thirst, hunger, perceived thirst and perceived hunger in relation to time and carbonated beverage consumption. Methods Participants (males n = 14, females n = 15) aged 23–65, had a BMI &amp;lt; 30 kg/m2, and no reported chronic disease. Participants completed six data collections, arriving four hours fasted and consuming one of six randomized beverages (water, carbonated-no flavor [CNF], carbonated lime flavor [CL], degassed lime flavor [DL], carbonated lime flavor with aspartame [CLS], and degassed lime flavor with aspartame [DLS]). Blood was collected via finger stick at 0 min (baseline), followed by beverage consumption, and again at 10 and 45 min. post consumption. Perceived hunger and thirst were measured by visual analog scale. Acylated ghrelin and copeptin concentrations were assessed by ELISA assay. Spearman's rank correlation coefficient was used to determine relationships. A repeated measures ANOVA was used to determine differences in ghrelin response. Results Perceived hunger scores differed by time (P &amp;lt; 0.0001) but not by beverage (P &amp;gt; 0.05) and there was no interaction. However, acylated ghrelin concentration did not differ by beverage or time (P &amp;gt; 0.05). Acylated ghrelin overall was 86.25 ± 92.30 pg/mL (mean ± SD). There was no relationship between perceived hunger and acylated ghrelin concentration at any time point or beverage (P &amp;gt; 0.05). Adjusting for gender or BMI had no impact. Perceived thirst differed by time (P &amp;lt; 0.0001) but not by beverage (P &amp;gt; 0.05). Copeptin concentrations are currently being analyzed via ELISA assay. Preliminary results for copeptin concentrations from participants consuming water and CNF did not differ by beverage or time. The average copeptin concentration measured from participants for these two beverages was 4.5 ± 4.0 ng/mL. After all samples are analyzed for copeptin, correlation will be run to determine if there is any relationship between copeptin, ghrelin, and perceived hunger and thirst. Conclusions Perceived hunger and thirst changed over time, regardless of beverage type. However, biomarker concentrations were not related to those changes. Perception of satiety may be influenced by other factors other than physiological signals. Funding Sources Funded by the College of Saint Benedict/Saint John's University Faculty Development Grant.