Activity Of Herbal Drugs Research Articles

Phytochemical Screening and In-Vitro Antioxidant Activity of An Herbal Drug ‘Ashwagandharishta’, Estimation of Total Phenolic and Flavonoid Contents

Because of their ability to neutralise free radicals, phenolic compounds have a variety of biological effects, including anti-inflammatory, antibacterial, and antioxidant characteristics. Antioxidants are essential nutrients that protect the body from the harmful effects of oxidative stress caused by free radicals. A wide range of free radical scavenging antioxidants can be found in a variety of foods. The study's goal was to find out how much total phenolic content, total flavonoids content, antioxidant activity, and phytochemical screening of herbal drugs. Flavonoids are a type of natural polyphenolic chemicals that humans are unable to produce. These compounds have a variety of biological characteristics and act as antioxidants in biological systems. The Folin-Ciocalteau method was used to determine the total phenolic content. Antioxidant properties were determined using 1,1-diphenyl-2- picrylhydrazyl (DPPH). The total phenolic and flavonoid content was determined spectrophotometrically using gallic acid and quercitin as standards. The Herbal drug for the study is purchased from the local markets of Nashik (Maharashtra). 5 percent, 2.5 percent, 1.25 percent, 0.625 percent, 0.3125 percent, and 0.15625 percent concentration samples in water of the herbal medication were prepared for the assessment of antioxidant potential by DPPH assay. As the concentration % rises, so does the antioxidant activity, as well as the overall phenolic and falvonoid content increases. The antioxidant activity is due to the existence of total phenolic and flavonoid content. Conventional methods were used to determine the presence of specific phytochemicals.

An ayurvedic tablet formulation for ulcer protection

Gastric ulcers or the peptic ulcer is the primary disease that affects the gastrointestinal system. A large extent of the population in the world are suffering from the disease, and the age group of people those who suffer from ulcers are 20-55years. Herbs are known to the human beings that are useful in the treatment of diseases, and there are a lot of scientific investigations that prove the pharmacological activity of herbal drugs. Practitioners have been using the herbal material to treat the ulcers successfully, and the same had been reported scientifically. Numerous publications have been made that proves the antiulcer activity of the plants around the world. The tablets were investigated for the antiulcer activity in two doses 200 and 400mg/kg in albino Wistar rats in the artificial ulcer those are induced by the ethanol. The prepared tablets showed a better activity compared to the standard synthetic drug and the marketed ayurvedic formulation. The tablets showed a dose-dependent activity in ulcer prevention and treatment. Many synthetic drugs are available for the ulcer treatment, and the drugs pose the other problems in the body by showing the side effects and some other reactions. This limits the use of synthetic drugs to treat ulcers effectively. Herbs are known to the human beings that are useful in the treatment of diseases, and there are a lot of scientific investigations that prove the pharmacological activity of herbal drugs.

Comparative characteristics antimicrobial activity of different classes of antibiotics and antimicrobial isolates on Staphylococcus aureus

Annotation. S. aureus is a leading cause of nosocomial infections in health care facilities. The aim of the study was to determine the effectiveness of the use of staphylococcal bacteriophage, phytopreparation “Chlorophyllipt”, of the drug “Protargol”, compared with antimicrobial chemotherapeutic drugs (ACD) against isolates of Staphylococcus aureus in vitro. Used clinical strains of S. aureus, isolated from 20 patients, aged 19 to 25 years. The susceptibility of microorganisms to ACD was determined on the MPA medium by disk-diffusion method. Staphylococcal bacteriophage sensitivity scores were evaluated by drainage lysis of bacterial indicator culture and negative phage colonies. Anti-staphylococcal activity of phytopreparation and protargol solution was determined by diffusion in agar with the isolates tested. Statistical data processing was performed using Microsoft Excel 2013. In determining the susceptibility of S. aureus isolates to ACD, 30% of penicillin-sensitive strains were detected. Amoxicillin sensitivity was 25% of strains. The rate of detected sensitivity of microorganisms to amoxiclav was 45% of strains. Sensitivity to cephalosporins I-II generation amounted to 75% cephalexin, cefazolin, cefuroxime — 90%. For cephalosporins of the third generation — from 70% to 95%; to macrolides: to clarithromycin — 80%, to erythromycin, azithromycin — 50%, 55% respectively. High sensitivity was reported for lincomycin — 80%. The studied strains showed absolute sensitivity to amikacin (100%). 95% strains were sensitive to gentamicin. Not exceed 60–65% sensitivity to tetracyclines and fluoroquinolones. All bacterial isolates were sensitive to bacteriophage. High antimicrobial activity in up to chlorophyllipt was detected in up to 8 test strains. 19 strains have had low-sensitivity to protargol. The results obtained indicate the feasibility of bacteriophages in the medical practice and the continuation of microbiological studies with the study of antimicrobial activity of herbal drugs.

Antioxidant Activity of Indian Herbal Drugs in Rats with Aloxan-induced Diabetes

Methanol extracts (75%) of Aegle marmelos, Momordica charantia, Trigonella foenum-graecum, Eclipta prostrata, Salacia oblonga, Coriandrum sativum, Vernonia anthelmintica and Murraya koenigii, plants used extensively in the Indian system of medicine, were found to be potent inhibitors of lipid peroxide formation and scavengers of hydroxyl and superoxide radicals in vitro. Oral administration of the extracts (100 mg/kg body weight) were found to reduce the serum glucose level in normal and alloxan (120 mg/kg) diabetic rats significantly for 4h and continued administration of the drug sustained this effect. Reduction of serum glucose by activities of herbal drugs such as Aegle marmelos (56.8%), Momordica charantia (55.4%) and Trigonella foenum-graecum (52.5%) were almost as high as the activity of insulin (60.4%). The extract of Aegle marmelos showed highest activity as an antidiabetic and antioxidant, compared to other extracts. The results indicated that oxidant induced alterations in the glucose utilizing system during diabetic manifestation is partially reversed by the administration of herbal extracts having antioxidant activity.