Activity In MT Research Articles

Good vision without peripheries: behavioral and fMRI evidence

In healthy vision, bright slow-motion stimuli are processed primarily by the regions of the visual system that receive input from the central part of the scene, whereas processing of dark fast-motion stimuli is more dependent on peripheral visual input. We tested 31 retinitis pigmentosa (RP) patients with long-term loss of peripheral photoreceptors and healthy controls with temporarily limited peripheral vision. We measured motion-based acuity using random-dot kinematograms, establishing individual thresholds for differentiating a circle from an ellipse. Participants subsequently performed a functional magnetic resonance imaging (fMRI) task set at a constant level of difficulty. The results showed that limiting vision did not affect motion-acuity thresholds in control participants but did cause different brain activations than those in RP patients, indicating prompt implementation of the strategy that would be perceptually successful. Compared with controls with both full and limited vision, impaired motion acuity in RP patients led to decreased brain activation, particularly in the primary peripheral visual areas V1-3. Importantly, compared with controls in full vision, matched decreased activation in MT+/V5, salience-processing cortices and the superior temporal cortex were detected in RP patients and in controls with limited peripheral vision, revealing brain networks that compensate for the loss of peripheral vision.

Abnormal activation patterns in MT+ during visual motion perception in major depressive disorder.

Previous studies have found that patients with Major Depressive Disorder (MDD) exhibit impaired visual motion perception capabilities, and multi-level abnormalities in the human middle temporal complex (MT+), a key brain area for processing visual motion information. However, the brain activity pattern of MDD patients during the perception of visual motion information is currently unclear. In order to study the effect of depression on the activity and functional connectivity (FC) of MT+ during the perception of visual motion information, we conducted a study combining task-state fMRI and psychophysical paradigm to compare MDD patients and healthy control (HC). Duration threshold was examined through a visual motion perception psychophysical experiment. In addition, a classic block-design grating motion task was utilized for fMRI scanning of 24 MDD patients and 25 HC. The grating moved randomly in one of eight directions. We examined the neural activation under visual stimulation conditions compared to the baseline and FC. Compared to HC group, MDD patients exhibited increased duration threshold. During the task, MDD patients showed decreased beta value and percent signal change in left and right MT+. In the sample comprising MDD and HC, there was a significant negative correlation between beta value in right MT+ and duration threshold. And in MDD group, activation in MT+ were significantly correlated with retardation score. Notably, no such differences in activation were observed in primary visual cortex (V1). Furthermore, when left MT+ served as the seed region, compared to the HC, MDD group showed increased FC with right calcarine fissure and surrounding cortex and decreased FC with left precuneus. Overall, the findings of this study highlight that the visual motion perception function impairment in MDD patients relates to abnormal activation patterns in MT+, and task-related activity are significantly connected to the retardation symptoms of the disease. This not only provides insights into the potential neurobiological mechanisms behind visual motion perception disorder in MDD patients from the aspect of task-related brain activity, but also supports the importance of MT+ as a candidate biomarker region for MDD.

Extracellular vesicles derived from dendritic cells loaded with VEGF-A siRNA and doxorubicin reduce glioma angiogenesis in vitro

BackgroundNumerous attempts have been devoted to designing anti-angiogenic agents as a strategy to slow tumor growth and progression. Clinical applications of conventional anti-angiogenic agents face some challenges, e.g., off-target effects for TKIs and also low solid tumor penetration for mAbs. Furthermore, although anti-angiogenic therapy provides a normalization window for better chemo-RT response, in long-term treatments, tumor hypoxia as a result of total removal of VEGF-A by mAbs from the TME or complete blockade of TK receptors induces over-activation of compensatory angiogenic pathways, causing escape. Herein, we investigate the efficacy of si-DOX-DC-EVs to reduce glioma angiogenesis and invasiveness. MethodsMature DCs were generated from PBMC and EVs were isolated from the DCs culture media. siRNA and Doxorubicin were loaded into EVs by EP and incubation. Afterward, the uptake of DC-EVs was assessed by flow cytometry, and the subcellular localization of EVs was tested by confocal imaging. Tube formation assay was performed to assess the efficacy of si-DOX-DC-EVs to reduce tumor angiogenesis which was analyzed by DHM. Morphometric analysis of apoptotic cells was performed by DHM and confocal imaging and further, ELISA was performed for hypoxia-related and angiogenic cytokines. The impact of our theranostic system “si-DOX-DC-MVs” on the formation of vascular mimics, colonies, and invasion of C6 cells was checked in vitro. Afterward, orthotropic rat models of glioma were generated and the optimal administration route was selected by in vivo fluorescent analysis. Then, the microvessel density, vimentin expression, and accumulation of immune cells in tumoral tissues were assessed by IHC. Finally, necropsy and autopsy analyses were performed to check the safety of our theranostic agent. ResultsDC-EVs loaded with si-DOX-DC-EVs were successfully uptaken by cells with different subcellular trafficking for MVs and exosomes, reduced tumor angiogenesis in DHM analysis, and induced apoptosis in tumoral cells. Moreover, using DHM, we performed a detailed label-free analysis of tip cells which suggested that the tip cells in si-DC-MV treatments lost their geometrical migration capacity to form tube-like structures. Furthermore, the ELISAs performed highlighted that there is a mild overactivation of compensatory Tie2/Ang2 pathway after VEGF-A blockade which confers with severe hypoxia and sustains normal angiogenesis which is the optimal goal of anti-angiogenesis therapy for cancer to avoid resistance.The results of our VM analyses indicated that si-DOX-DC-MVs completely inhibited VM process. Moreover, the invasion, migration, and colony formation of the C6 cells treated with si-DOX-MVs were the least among all treatments. IN was the optimal route of administration. The MVD analyses indicated that si-DOX-DC-MVs reduced the number of tumoral microvessels and normalized vessel morphology. Intense CD8+ T cells were observed near the tumoral vessels in the si-DOX-DC-MVs group and with minimal activation of MT (low Vimentin expression). Necropsy and toxicology results proved that the theranostic system proposed is safe. ConclusionsDC-EVs loaded with VEGF-A siRNA and Doxorubicin were more potent than BV alone as a multi-disciplinary strategy that combats glioma growth by cytotoxic impacts of DOX and inhibits angiogenesis by VEGF-A siRNAs with excess immunologic benefits from DC-EVs. This next-generation anti-angiogenic agent normalizes tumor vessel density rather than extensively eliminating tumor vessels causing hypoxia and mesenchymal transition.

Expression and role of melatonin membrane receptors in the hypothalamic-pituitary-testicular axis of Tibetan sheep in a plateau pastoral area.

MTNR1A and MTNR1B, two high-affinity MT membrane receptors found in mammals, mediate the activity of MT on the HPGA to regulate animal reproduction. Nevertheless, the expression patterns and function of the MTNR1A and MTNR1B genes in the HPTA of seasonal estrus sheep and perennial estrus sheep have not been elucidated. We studied the expression of MTNR1A and MTNR1B in the hypothalamic-pituitary-testicular axis (HPTA) of Tibetan sheep at different reproductive stages using histochemistry, enzyme linked immunosorbent assay (ELSIA), scanning electron microscopy, transmission electron microscopy, quantitative Real-time PCR (qRT-PCR), and Western blot (WB), and analyzed the relationship between their expression and reproductive hormone receptors. We also compared relevant characteristics between seasonal Tibetan sheep and non-seasonal Small Tail Han sheep in the same pastoral area. The results showed that MTNR1A and MTNR1B were expressed in all tissues of the Tibetan sheep HPTA, and both were co-expressed in the cytoplasm of epididymis basal and halo cells located at common sites of the epididymis basement membrane, forming an immune barrier. The qRT-PCR analysis showed that not only MTNR1A but also N-acetyltransferase (AANAT), hydroxyindole-oxygen- methyltransferase (HIOMT), androgen receptor (AR), and estrogen receptor α (ERα) mRNA expression was significantly upregulated in the testis and epididymis of Tibetan sheep during the breeding season, whereas no clear upregulation of these genes was observed in the tissues of Small Tail Han sheep. MTNR1A and MTNR1B are important regulators of the HPTA in sheep. MTNR1A mediates seasonal estrus regulation in Tibetan sheep. Both MTNR1A and MTNR1B may play important roles in formation of the blood-epididymal barrier. The results of this study should help advance research on the mechanism of reproductive regulation of the HPTA in male animals and provide reference data for improving the reproductive rate of seasonal breeding animals.

Functional changes in fusional vergence-related brain areas and correlation with clinical features in intermittent exotropia using functional magnetic resonance imaging.

To explore the functional changes of the frontal eye field (FEF) and relevant brain regions and its role in the pathogenesis of intermittent exotropia (IXT) children via functional magnetic resonance imaging (fMRI). Twenty-four IXT children (mean age, 11.83 ± 1.93 years) and 28 normal control (NC) subjects (mean age, 11.11 ± 1.50 years) were recruited. During fMRI scans, the IXT children and NCs were provided with static visual stimuli (to evoke sensory fusion) and dynamic visual stimuli (to evoke motor fusion and vergence eye movements) with binocular disparity. Brain activation in the relevant brain regions and clinical characteristics were evaluated. Group differences of brain activation and brain-behavior correlations were investigated. For dynamic and static visual disparity relative to no visual disparity, reduced brain activation in the right FEF and right inferior occipital gyrus (IOG), and increased brain activation in the left middle temporal gyrus complex (MT+) were found in the IXT children compared with NCs. Significant positive correlations between the fusional vergence amplitude and the brain activation values were found in the right FEF, right IPL, and left cerebellum in the NC group. Positive correlations between brain activation values and Newcastle Control Scores (NCS) were found in the left MT+ in the IXT group. For dynamic visual disparity relative to static visual disparity, reduced brain activation in the right middle occipital gyrus, left cerebellum, and bilateral IPL was found in the IXT children compared with NCs. Significant positive correlations between brain activation values and the fusional vergence amplitude were found in the right FEF and right cerebellum in the NC group. Negative correlations between brain activation values and NCS were found in the right middle occipital gyrus, right cerebellum, left IPL, and right FEF in the IXT group. These results suggest that the reduced brain activation in the right FEF, left IPL, and cerebellum may play an important role in the pathogenesis of IXT by influencing fusional vergence function. While the increased brain activation in the left MT+ may compensate for this dysfunction in IXT children.

Effect Of Different Grip Position And Shoulder- Abduction Angle On Muscle Strength And Activation During The Seated Cable Row

The purpose of this study was to compare the peak force and electromyographic activation in different techniques of the seated row. Eleven recreationally trained male (28±5 years, 176±5 cm, 94±16 kg) and ten female (27±5 years, 168±8 cm, 65±11 kg ) performed an isometric and a dynamic assessment of the seated cable row and the preacher curl: pronated grip (PRO), supinated grip (SUP), neutral grip (NEU), 30° (30°), 60° (60°), 90° (90°) of shoulder abduction, and cable preacher curl (PC). Peak force and muscle activation of upper trapezius, middle trapezius, upper latissimus, lower latissimus, posterior deltoid, and biceps bracii were recorded during the isometric and dynamic assessment of the exercises. p values<0.05 were considered statistically significant. The peak force was greater when shoulder abduction angle was closer to 0° (PRO, SUP, and NEU) and decreased as abduction angle increased (60° and 90°). Muscle activation of the upper back (upper trapezius, middle trapezius) and posterior deltoid increased as abduction angle increased (60° and 90°) on both isometric and dynamic analysis. Muscle activation of the upper and lower latissimus increased as abduction angle was closer to 0° (PRO, SUP, and NEU) on both isometric and dynamic analysis. Biceps brachii activation on both isometric and dynamic analysis during the PC was greater than all other rowing conditions; among the rowing techniques, BB activation was greater during SUP, NEU, and 90° techniques. In conclusion, the closer to 90° shoulder abduction the greater UT, MT, and PD activity, on the other hand, the closer to 0° shoulder abduction the greater peak force, UL, and LL activity during the variations of the rowing exercise.

Hepatic Insulin Resistance Model in the Male Wistar Rat Using Exogenous Insulin Glargine Administration.

Metabolic diseases are a worldwide health problem. Insulin resistance (IR) is their distinctive hallmark. For their study, animal models that provide reliable information are necessary, permitting the analysis of the cluster of abnormalities that conform to it, its progression, and time-dependent molecular modifications. We aimed to develop an IR model by exogenous insulin administration. The effective dose of insulin glargine to generate hyperinsulinemia but without hypoglycemia was established. Then, two groups (control and insulin) of male Wistar rats of 100 g weight were formed. The selected dose (4 U/kg) was administered for 15, 30, 45, and 60 days. Zoometry, a glucose tolerance test, insulin response, IR, and the serum lipid profile were assessed. We evaluated insulin signaling, glycogenesis and lipogenesis, redox balance, and inflammation in the liver. Results showed an impairment of glucose tolerance, dyslipidemia, hyperinsulinemia, and peripheral and time-dependent selective IR. At the hepatic level, insulin signaling was impaired, resulting in reduced hepatic glycogen levels and triglyceride accumulation, an increase in the ROS level with MAPK-ERK1/2 response, and mild pro-oxidative microenvironmental sustained by MT, GSH, and GR activity. Hepatic IR coincides with additions in MAPK-p38, NF-κB, and zoometric changes. In conclusion, daily insulin glargine administration generated a progressive IR model. At the hepatic level, the IR was combined with oxidative conditions but without inflammation.

Using biomarkers to assess the toxicity of environmental concentrations of heavy metals to the marine gastropod Thais mutabilis

This study investigated the effects of environmentally relevant concentration of heavy metals on the antioxidant, metabolic, and detoxifying enzymes activity of marine gastropod Thais mutabilis in Bandar Abbas (north of Persian Gulf). In this study, gastropod samples were collected from five transects in the coastal line, and in each transect, three sampling points were selected. Sampling was conducted in both cold season (autumn 2020) with temperatures of 13–25 °C and warm season (summer 2021) with temperatures of 29 and 40 °C, respectively. Sampled animals had an average weight of 15.38 ± 3.75 and 13.54 ± 4.44 g in cold and warm seasons, respectively. Soft tissues of T. mutabilis were collected and dried using the freeze drier. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to evaluate the concentration of heavy metals (Al, As, Ba, Fe, Pb, Cu, Cr, Cd, Hg, Mn, Ni, Ti, V, Zn). The activity of metallothionein, superoxidase dismutase, and Glutathione S-transferase were examined and possible correlations between heavy metals and enzymes were tested through this study. Results showed that the concentration of heavy metals in the body of T. mutabilis exceeded the values suggested by WHO, FAO, and USEPA. Heavy metals concentration showed the highest values for Fe and Al at transect 1 (cold and warm seasons), As, Ba and Cu at transect four (cold and warm seasons), Ni, Cr and Hg at transect four (warm season). SOD showed the highest activity at transect five in warm and cold seasons at 69.50 ± 9.59 and 64.88 ± 13.92 U/g protein, respectively. Transect three had the greatest values for SOD levels in warm and cold seasons at 41.50 ± 7.58 and 48.01 ± 20.60 U/g protein, respectively. GST demonstrated the largest activity at transect five in warm and cold seasons at 3.76 ± 1.15 U/g protein. MT had the highest levels at transect five in cold and warm seasons at 1.79 ± 0.30 and 1.96 ± 0.29 U/g protein. Oxidative, metabolic and detoxifying enzymes play a leading role as viable biomarkers in assessing the toxicity of hazardous compounds. In this study significant correlations were observed between MT activity and heavy metals (Pb, Al, Ba, Fe, and Zn) as well as SOD and Fe, so T.mutabilis could be a good bioindicator in the polluted marine biota.

Clay-Catalyzed Ozonation of Hydrotalcite-Extracted Lactic Acid Potential Application for Preventing Milk Fermentation Inhibition

An unprecedented route for mitigating the inhibitory effect of lactic acid (LA) on milk fermentation was achieved through lactate adsorption on hydrotalcite (Ht) from simulated lactate extracts. During its regeneration by ozonation, Ht displayed catalytic activity that appeared to increase by addition of montmorillonite (Mt). Changes in the pH, Zeta potential and catalyst particle size during LA ozonation were found to strongly influence LA–LA, LA–catalyst and catalyst–catalyst interactions. The latter determine lactate protonation–deprotonation and clay dispersion in aqueous media. The activity of Mt appears to involve hydrophobic adsorption of non-dissociated LA molecules on silica-rich areas at low pH, and Lewis acid–base and electrostatic interactions at higher pH than the pKa. Hydrotalcite promotes both hydrophobic interaction and anion exchange. Hydrotalcite–smectite mixture was found to enhance clay dispersion and catalytic activity. This research allowed demonstrating that natural clay minerals can act both as adsorbents for LA extract from fermentation broths and as catalysts for adsorbent regeneration. The results obtained herein provide valuable and useful findings for envisaging seed-free milk clotting in dairy technologies.

Altered mitochondria oscillations and circadian changes in NADH levels in freshwaters mussels exposed to cadmium

The effects of contaminants on biological rhythms are largely unknown. The purpose of this study was to examine the effects of cadmium (Cd) and time of day on levels of defence mechanism (metallothionein-MT), mitochondria NADH oxidase activity and oscillations in NADH levels in freshwater mussels Dreissena bugensis. Following exposure of mussels to 50 and 100 μg/L Cd for 7 days, mussels were sampled at various times of the day (9, 12, 15, 18, 21 and 24 h) and immediately stored at −85 °C. Tissues were analyzed for MT levels, NADH oxidase and oscillatory changes in NADH in mitochondria assessments. The data revealed that exposure to Cd readily increased MT and NADH oxidase activity at each time of day. In control mussels, NADH oxidase activity changed during the day reaching a minimum and maximum at 15 h and 24 h respectively as with MT levels but the maximum occurred at 12 h. Exposure to Cd displaced the maximum values in NADH oxidase towards the night (≥18 h) while no change in the pattern in MT changes during the day albeit remained at high levels. NADH oscillations in mitochondria were complex but main changes occurred at periods of 60–90 s in control mussels. The strength of association (β) between amplitudes and frequencies of these oscillations was negatively associated with MT levels suggesting that activation of defence mechanism limited the propagation of amplitude changes at higher times. The study revealed that Cd exposure could disrupt not only the intensity of responses but at the levels of rhythms commonly found in biological systems.

Scapulothoracic muscle activity during kinetic chain variations of a prone elevation exercise

BackgroundScapular rehabilitation exercises should target appropriate muscles. Recently, adding external rotation resistance to scapular exercises has gained interest. Moreover, clinical experts advise kinetic chain integration into shoulder rehabilitation exercises. ObjectiveTo investigate scapular muscle activity during kinetic chain variations of a prone elevation exercise. MethodsActivity of the upper (UT), middle (MT), and lower (LT) trapezius and serratus anterior (SA) muscles was determined with surface electromyography (EMG) in 31 asymptomatic participants during six prone elevation exercise variations. Variation was created by adding external rotation resistance, adding trunk extension, or changing exercise position (prone on a Swiss ball with knees or feet supported, or prone on a physiotable). All data were normalized as a percentage of maximal voluntary isometric contraction (% MVIC). For each muscle, a Friedman's ANOVA was conducted to analyse statistical differences in EMG signal intensity between exercises. ResultsThe LT was moderately (42 - 48% MVIC) and MT highly (63 - 66% MVIC) activated during all exercise variations. No significant differences between exercises for these muscles could be detected. Adding external rotation to a prone elevation exercise decreased UT activity while adding trunk extension increased UT activity. Altering exercise position had no influence on scapular muscle activity except increased UT activity when lying prone on a physiotable with trunk extension. ConclusionProne elevation exercises are appropriate for facilitating LT and MT activity. Adding external rotation inhibits UT activity while UT facilitation could be achieved when adding trunk extension.

A 1.5 Ma Marine Record of Volcanic Activity and Associated Landslides Offshore Martinique (Lesser Antilles): Sites U1397 and U1399 of IODP 340 Expedition

The products of eruptive and mass-wasting processes that built island arc volcanoes are better preserved in marine deposits than on land. Holes U1397A and U1399A drilled during IODP Expedition 340 provide a 1.5 Ma record of the volcanic history of Martinique. 14C dating and δ18O patterns are used to reconstitute the chronostratigraphy of tephra, volcaniclastic turbidites, and mass-wasting events (traced by debris avalanches, debrites, and duplication and deformation of pre-existing sediments), leading to a new volcanic history of Montagne Pelée and Pitons du Carbet volcanoes. The top 50 m of core U1397A provides a continuous high-resolution sedimentation record over the last ∼130 ka. The sedimentation record deeper than 50 m in core U1397A and in the whole core U1399A is discontinuous because of the numerous sliding and deformation events triggered by debris avalanches related to flank collapses. Three successive activity periods are identified since ∼190 ka: the “Old Pelée” until 50 ka, the “Grand Rivière” (50–20 ka), and the “Recent Pelée” (20 ka—present day). The first two periods have the highest volcanic deposition rates offshore but very little outcrop on land. The whole magmatic activity of Mt Pelée comprises silicic andesites, but mafic andesites were also emitted during the whole “Grand Rivière.” At ∼115 ka, a major flank collapse (“Le Prêcheur”) produced a debris avalanche and submarine landslide that affected sea floor sediments by erosion and deformation up to ∼70 km from the shore. The Pitons du Carbet volcano was active from 1.2 Ma to 260 ka with numerous large flank collapses at a mean rate of 1 event every 100 ka. The average deposition rate of tephra fall offshore is much less than that at Mt Pelée. Our data show that correlations between the timing of large landslides or emission of mafic magmas and rapid sea level rise or lowstands suggested by previous studies are not systematic. The reconstituted chronostratigraphy of cores U1397A and U1399A provides the framework necessary for further studies of the magma petrology and production rates and timing of the mechanisms triggering flank collapses and related submarine landslides of Mt Pelée and Pitons du Carbet.

Effect of ionic Fe(III) doping on montmorillonite for photocatalytic reduction of Cr(VI) in wastewater

The potential photocatalytic activity of Mt (montmorillonite) after Fe(III) doping was measured for the photocatalytic remediation of hexavalent chromium (Cr(VI)). Mt was modified with Fe(III) at pH = 2 with IAP ≤ 1.5 ∙ 10–38 <Ksp(Fe(OH)3) = 6.0 ∙ 10 -38 to prevent the precipitation of Fe(OH)3. Doped ionic Fe(III) was located mainly in the interlayer space but presented partially on the Mt layers. XRD, FTIR, XPS and XANES/EXAFS analyses suggested that Fe(III) should exist in the cationic form. The photocatalytic characterization of the obtained Fe/ Mt suggests that Mt gained photocatalytic activity after Fe(III) ion doping. The photocatalytic transformation of Cr(VI) to harmless trivalent chromium (Cr(III)) on Fe/ Mt was also dominated by Fe(III) doping on the Mt interlayer and Mt aluminosilicate layers. Higher Fe(III) doping correlated with higher photocatalytic efficiency with the Fe(III) adsorbed on Mt at a ratio lower than 0.274 mmol/g. A further intercalation of Fe(III) reduced the photocatalytic efficiency, which could be attributed to the excess Fe(III) providing a recombination state for the electrons, trapping the electrons from the Cr(VI) reduction, and the extra Fe(III) overlapping with the active sites receiving irradiation. Based on the characterization and photocatalytic activity test, the mechanism of the photocatalytic property of Fe(III)-doped Mt was revealed for the first time. The new Fermi level formed between the forbidden bands narrowed the band gap of Mt, endowing the Mt with photocatalytic activity. This finding may contribute to explaining the role of Fe/ Mt in Fe/ Mt-based photocatalytic composites.

Discussion of “Quantitative evaluation of thixotropy-governed microfabric evolution in soft clays”

The diagenesis of clay minerals and thermal evolution of organic matter (OM) are consistent in space and time in most petroliferous basins. Within these processes, the synchronism of smectite illitization with peak hydrocarbon generation is one of the most important phenomena and the illitization process is believed to play an important role in catalytic effects on the conversion of OM to oil and gas. The variation in the layer charge of montmorillonite (Mt) is a key indicator of illitization. However, the effects of layer charge on OM pyrolysis have not been investigated. In this study, a series of homologous reduced-charge Mt (LiMt) samples were prepared by changing the temperature of heat treatment based on the Hofmann–Klemen effect. The 12-aminolauric acid and Mt were mechanically mixed to prepare clay–OM complexes. Pyrolysis experiments were conducted in a confined gold capsule system, and the gas components were evaluated by gas chromatography. The experimental results show charge reducing changed the solid acidity of Mt and further influence the catalytic activity of Mt on the OM pyrolysis. The solid acidity of Mt first increased and then decreased as the layer charge decreased, while the water content decreased. These sites, especially Brønsted (B) acid sites, catalyzed OM pyrolysis via the carbocation mechanism. The content of iso‐alkanes was controlled by the content of water in Mt because excess water molecules hindered the isomerization reactions occurring at the B acid sites. The CO2 content was affected by both the amount of Lewis (L) acid sites and the water content. These fundamental results suggest that changes of the layer charge in Mt which occurs during the processes of smectite illitization may play an important role in hydrocarbon generation. These findings are important for understanding the organic-inorganic interactions between clays and OM during sedimentation and diagenesis.

Effects of montmorillonite charge reduction on the high-temperature/high-pressure pyrolysis of organic matter

The diagenesis of clay minerals and thermal evolution of organic matter (OM) are consistent in space and time in most petroliferous basins. Within these processes, the synchronism of smectite illitization with peak hydrocarbon generation is one of the most important phenomena and the illitization process is believed to play an important role in catalytic effects on the conversion of OM to oil and gas. The variation in the layer charge of montmorillonite (Mt) is a key indicator of illitization. However, the effects of layer charge on OM pyrolysis have not been investigated. In this study, a series of homologous reduced-charge Mt (LiMt) samples were prepared by changing the temperature of heat treatment based on the Hofmann–Klemen effect. The 12-aminolauric acid and Mt were mechanically mixed to prepare clay–OM complexes. Pyrolysis experiments were conducted in a confined gold capsule system, and the gas components were evaluated by gas chromatography. The experimental results show charge reducing changed the solid acidity of Mt and further influence the catalytic activity of Mt on the OM pyrolysis. The solid acidity of Mt first increased and then decreased as the layer charge decreased, while the water content decreased. These sites, especially Brønsted (B) acid sites, catalyzed OM pyrolysis via the carbocation mechanism. The content of iso‐alkanes was controlled by the content of water in Mt because excess water molecules hindered the isomerization reactions occurring at the B acid sites. The CO2 content was affected by both the amount of Lewis (L) acid sites and the water content. These fundamental results suggest that changes of the layer charge in Mt which occurs during the processes of smectite illitization may play an important role in hydrocarbon generation. These findings are important for understanding the organic-inorganic interactions between clays and OM during sedimentation and diagenesis.

L\xe9vy walk dynamics explain gamma burst patterns in primate cerebral cortex

Lévy walks describe patterns of intermittent motion with variable step sizes. In complex biological systems, Lévy walks (non-Brownian, superdiffusive random walks) are associated with behaviors such as search patterns of animals foraging for food. Here we show that Lévy walks also describe patterns of oscillatory activity in primate cerebral cortex. We used a combination of empirical observation and modeling to investigate high-frequency (gamma band) local field potential activity in visual motion-processing cortical area MT of marmoset monkeys. We found that gamma activity is organized as localized burst patterns that propagate across the cortical surface with Lévy walk dynamics. Lévy walks are fundamentally different from either global synchronization, or regular propagating waves, because they include large steps that enable activity patterns to move rapidly over cortical modules. The presence of Lévy walk dynamics therefore represents a previously undiscovered mode of brain activity, and implies a novel way for the cortex to compute. We apply a biophysically realistic circuit model to explain that the Lévy walk dynamics arise from critical-state transitions between asynchronous and localized propagating wave states, and that these dynamics yield optimal spatial sampling of the cortical sheet. We hypothesise that Lévy walk dynamics could help the cortex to efficiently process variable inputs, and to find links in patterns of activity among sparsely spiking populations of neurons.

Antivirulence activity and in vivo efficacy of a thiazole derivative against candidiasis

Candida albicans is a pathogen equipped with a variety of commensal and virulence traits that help it colonize the microbiota and invade host tissue during infection. In this study, we investigated the potential anticandidal activity of 3-[2-(4-(4-methoxyphenyl)thiazol-2-yl)hydrazino)]butan-1-ol (MT), a thiazolylhydrazone compound synthesized by our group, and identified it as a promising antifungal agent. The activity of MT was evaluated in vitro and in vivo against C. albicans as well as its ability to inhibit virulence factors. For this, the ability of MT to inhibit the adhesion of C. albicans to human buccal epithelial cells and biofilm formation and filamentation was tested. In addition, the potential in vivo activity of MT was evaluated in murine models of oral candidiasis.Our results confirmed the antifungal activity of MT, with a minimal inhibitory concentration range of 0.5–2 µg/mL. Indeed, MT treatment in vitro decreased the expression of C. albicans genes involved in biofilm formation and morphogenesis and encoding hydrolytic enzymes, which was also confirmed through phenotypic observations. In addition, MT promoted a decrease in the colony forming units recovered from the tongues of mice with oral candidiasis. In this work, we present a potent antivirulence compound that shows potential for candidiasis therapy, especially for topical use.

Incorporation of the Kinetic Chain Into Shoulder-Elevation Exercises: Does It Affect Scapular Muscle Activity?

Scapular rehabilitation exercises should focus on selective activation of weaker muscles and minimal activation of hyperactive muscles. For rehabilitation of overhead athletes, single-plane open chain exercises below 90° of shoulder elevation are often recommended. Moreover, incorporating the kinetic chain in shoulder rehabilitation exercises is advised and has been suggested to influence scapular muscle activity levels. To study the influence of kinetic chain incorporation during 5 variations of a shoulder-elevation exercise on scapular muscle activity. Cross-sectional study. University laboratory. Thirty-one asymptomatic participants (15 men, 16 women). The electromyographic activity of the upper (UT), middle (MT), and lower (LT) trapezius, and serratus anterior was determined during 5 variations of bilateral elevation with external rotation: (1) open-hand position (reference exercise), (2) closed-hand position, (3) dynamic bipedal squat, (4) static unipedal squat, and (5) dynamic unipedal squat on the contralateral leg. All data were normalized as a percentage of maximal voluntary isometric contraction (MVIC). A closed-hand position (exercise 2) instead of an open-hand position (exercise 1) resulted in lower MT (mean difference = 3.44% MVIC) and LT (mean difference = 7.76% MVIC) activity. Incorporating the lower limb (exercises 3-5) increased UT activity when compared with exercise 1 (mean differences = 3.67, 2.68, 5.02% MVIC, respectively), which in general resulted in increased UT : MT ratios. Additionally, LT activity decreased when a dynamic unipedal squat was added (mean difference: 4.90% MVIC). For the serratus anterior, the greatest activity occurred during elevation in a static unipedal squat position (exercise 4, 22.90% MVIC). Incorporating the kinetic chain during shoulder-elevation exercises influenced scapular muscle activity and ratios. In particular, incorporating the lower limb resulted in more UT activity, whereas the open-hand position increased MT and LT activity.

Relative Scapular-Muscle Ratios During Maximal Isokinetic Shoulder-Girdle Strength Performance in Elite Field Hockey Players.

The shoulder joint and girdle are highly loaded during field hockey play. To optimize performance and to better substantiate preventive programs, it is important to gain insight into shoulder-girdle muscle function and balance in this athlete population. To evaluate relative scapular muscle-activity ratios through surface electromyography during maximal isokinetic strength testing in elite male field hockey players compared with nonathletes. Cross-sectional study. Institutional laboratory. Twenty-five elite field hockey players from the Belgian National Team and 25 age- and sex-matched nonathletes. We measured bilateral activity in 4 scapular muscles (upper trapezius [UT], middle trapezius [MT], lower trapezius [LT], and serratus anterior [SA]) during an external-internal rotation and protraction-retraction isokinetic shoulder protocol. Relative scapular muscle-activity ratios, or balance ratios, of the UT : MT, UT : LT, UT : SA, SA : MT, and SA : LT. We noted lower ratios bilaterally in the athlete group compared with the control group for the UT : MT, UT : LT, and SA : MT ratios during protraction, retraction, and external rotation, respectively, and unilaterally (dominant side only) for the UT : LT ratio during protraction. No consistent trend was present for established side differences in the studied balance ratios. Compared with nonathletes, elite field hockey players had altered intramuscular (within the trapezius) and intermuscular (between the trapezius and SA) balance ratios during maximal shoulder-girdle contractions, with relatively more MT and LT activity. This may reflect a sport-specific adaptation to optimize coordinated activity of the scapulothoracic muscles, meeting the specific demands of field hockey movements and simultaneously better protecting the shoulder against injury. Our results can assist in optimizing high-performance training and in supporting injury-prevention programs, which are key to both successful and long-lasting athletic careers.

Periscapular activity in subjects with scapular dyskinesis during push-ups on stable and unstable support surfaces

BACKGROUND: The scapular dyskinesis has been described as a sign of scapular instability due to weakness or imbalance of scapular muscles. OBJECTIVE: The aim of this study was to compare the EMG activity of the periscapular and abdominal muscles of asymptomatic subjects without and with scapular dyskinesis type II during a push-up exercise performed on different surface stability conditions. METHODS: Twenty-seven physically active men were allocated into two groups: the Control group (n= 14); and the Dyskinesis group (n= 13). All participants performed three variations of the push-up exercise: Stable, Push-up with hand instability, and Push-up with hand and feet instability. The EMG activity of serratus anterior, upper (UT), middle (MT) and lower (LT) trapezius, external oblique (EO) and rectus abdominis (RA) was recorded during each task. RESULTS: The control group showed an increase in MT activity (mean difference -3.46 to 8.66) under both instability conditions compared to stable push-ups (p&lt; 0.001). The comparison between groups showed that the control group had higher MT activity (mean difference -10.07 to 13.82) compared to the dyskinesis group under unstable conditions (p&lt; 0.012). CONCLUSION: The insertion of unstable surfaces increased MT activity in the control group only. The insertion of the unstable surface, either the hands or the footrest, did not provide significant effects for the other muscles.