Activity Of Different Agents Research Articles

Assessment of suppliers’ collaboration on construction project in Ebonyi state

In Nigeria, the concept of suppliers’ collaboration is still a new area to construction stakeholders. The construction companies in Ebonyi State are faced with enormous challenges which involve the integration of all construction processes and activities of different agents and specialists hired by clients for successful project delivery. To address the problem of construction project in Ebonyi State, construction stakeholders require collaboration which suppliers’ collaboration is the most pertinent. The aim is to assess the factors affecting contractors–suppliers relationship on successful construction project delivery in Ebonyi State. The general objective is to identify and determine the factors affecting contractors–suppliers relationship in construction industry and impact of supplier’s collaboration on the cost of construction project in Ebonyi State. The research sought to ensure at least 95% level of confidence with a total population of 400 and sample size set to 250. Primary and secondary sources were used to generate data with the aid of structured questionnaire and oral interviews directed to both private firms and public firms in construction industry. In total, 250 forms containing a set of question were submitted randomly to selected medium and large-sized construction firms in Ebonyi State. Data collected were presented in tables containing frequencies of the responses and their corresponding percentages. Frequency distribution, relative importance index (R11), Chi-square (X2), Python spyder IDE, statistical package for social science students(SPSS) and Pearson product moment correlation coefficient were the analytical tools used in the study. The result of the analyses revealed that, correlation matrix used to measure the relationship between contractors and supplier variables. The matrix depicts there is a linear correlation between all possible pairs about contractors and suppliers. There exist a relationship between contractors and suppliers as shown with other pairs in the correlation matrix that connects to objective number 3 and addresses the research question number 3. This implies that as the contractor-supplier approach short term or/and long term increases, the supplier collaboration on the cost of construction project in the Ebonyi State is expected to increase. The study concludes that the main drivers of the success of supplier collaboration appear to be the elements of trust and teamwork. It is these which ensure the continued relationship and ensure that both parties derive the benefits necessary for the partnership to continue and therefore; made the following recommendation. A detailed schedule of materials supply should be provided by the contractor. The schedule should contain the time required for material to arrive on site. Thus, results in reducing unproductive time on site and cost saving.

Antimicrobial activities of different agents including pyrophyllite against foodborne pathogens: A brief review

There has been worldwide an increasing interest and more strict criteria for food/feed safety including absence or reduction of the total number of microorganisms (bacteria, moulds and yeasts). Besides heavy metals, materials of biological origin (plant extracts, bio waste, chitosan etc.), some mineral adsorbents also have antimicrobial properties. There is much information about the antibacterial activity of the modified bentonite, montmoriollonite, smectute, zeolites and antifungal activity of various metal ion-exchanged zeolites and natural mineral clay, but there is almost no information about the antimicrobial properties of pyrophyllite, a monoclinic mineral from the group of phyllosilicates. This work summarizes the recent developments of antimicrobial agents and their application, current research, and trends in the area, highlighting pyrophyllite and its potential applications. Pyrophyllite, an unexploited mineral, possesses antimicrobial properties such as antibacterial and antifungal activities against foodborne pathogens which contributes to the protection of consumer?s health and preservation of the environment. Results from preliminary investigations indicate that pyrophyllite showed antibacterial properties against Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, and antifungal properties against fungal pathogens (Fusarium oxysporum, Phoma glomerata and Rhizoctonia solani). This mineral can also be used for biological control of F. oxysporum in the soil for growing potato.

Small prey exploitation during the final Gravettian in the northeast of the Iberian Peninsula: The case of Level D from Arbreda Cave

AbstractArbreda Cave is one of the most important sites in the Reclau Caves complex (Serinyà, north‐east Iberian Peninsula). Its stratigraphy reveals that the cave was intensively occupied during the Upper Palaeolithic. As in other archaeological sites in the western Mediterranean regions, a shift towards an intensification in small prey exploitation has been observed over the course of this period. This trend is indicated by the presence of large quantities of small mammal remains, mainly rabbits, in the archaeofaunal assemblages. However, the anthropogenic origin of these bone accumulations requires to be proved through the detailed taphonomic analysis because their presence in the archaeological record may be consequence of the activity of different agents. On this question, neotaphonomic studies have become a valuable tool with which to distinguish the taphonomic signatures produced by terrestrial carnivores, birds of prey, and humans.Although most researchers agree on the importance of small prey in the human diet during this period, this issue has not yet been resolved. Because data are still scarce, subsistence in some Upper Palaeolithic episodes, such as the final Gravettian, is not well understood. The aim of this paper is to establish the origin of the small mammal prey assemblages from Level D of Arbreda Cave and to assess the importance of these animals for the subsistence of humans in western Mediterranean regions. This level has been dated to ca. 25–20 kyr 14C BP and is mainly contemporary with the palaeoenvironmental cold conditions derived from the Greenland Stadial 3. The results of this study indicate that Level D small mammal prey assemblages have a remarkable anthropogenic footprint. Rabbits were intensively exploited, but other small mammals, such as hedgehogs, were also part of the human diet. The animal carcasses were brought whole to the cave, where they were butchered, cooked, and consumed. In addition, marrow exploitation was also practiced in the case of the rabbits.Although at Arbreda Cave, ungulates were a primary resource in the subsistence of hunter–gatherers; small prey also played an important role in human diet during the final Gravettian.

Characterization of the Brazilian vegetable brassicas production chain

ABSTRACT Brassica crops worldwide provide the greatest diversity of products used by man derived from a single genus. Collectively, leafy, flower and root vegetables can be eaten fresh, cooked and processed. The production of these vegetables in Brazil has been growing exponentially in the last decades, due to its economic importance, with major production volumes in several states and a quick monetary return for growers in areas considered of small acreage. With expansion, a number of aspects, including demands for quality and safety of these foods introduce themselves, strictly attached to the forms of production, logistics and their appearance. To meet the challenges and list the trends within the activities of different agents, a multiple case study was held in the states of Amazon, Minas Gerais, Paraná and Rio de Janeiro. Altogether, 55 stakeholders were interviewed, along with the compilation of different sources of data to characterize this productive chain. Changes in the scenario are imperative in order to maintain the activity of overcoming years, being related to cultivars adaptation, plant health, productions costs and difficulties during the establishment of crops. To overcome these adversities, a collective effort in terms of organization, technology transfer and R&D from official and private agents, technical assistance, and regional sanitary inspection is imperative, aiming, especially, in actions regarding themes like protected cultivation, mechanization/automation and adding value to products by processing and packaging, listed by stakeholders as priority.

Selection of Test Systems for Estimation of Anti-aggregation Activity of Molecular Chaperones

Searching the agents that are capable of effectively suppressing protein aggregation and elaboration of the corresponding test systems are among the most important problems of modern biochemistry and analytical biochemistry [1-7]. To characterize the anti-aggregation activity of different agents including chaperones of protein nature and low-molecular-weight chemical chaperones, test systems where the initial state of a target protein is the native protein that undergoes unfolding and subsequent aggregation under the action of different factors are mostly used. Unfolding of the target protein can be caused by heating at elevated temperatures. For several proteins the unfolding process can be initiated by the cleavage of S-S bonds in the protein molecule. Dithiothreitol (DTT)-induced aggregation was demonstrated, for example, for α-lactalbumin, insulin, lysozyme and bovine serum albumin (BSA).

The use of human tissue in drug discovery

Experiments conducted on human tissue samples are a key component of modern drug discovery programs and complement the use of animal tissue based assays in this process. Such studies can (i) enhance our understanding of disease pathophysiology, (ii) increase (or decrease) confidence that modulating the function of particular molecular targets will have therapeutic benefit (iii) allow comparison of the activities of different agents on particular mechanisms/processes and (iv) provide information on the potential safety risks associated with targets. All of this information is critical in identifying the targets that are most likely to deliver efficacious and safe medicines to address unmet clinical needs. With the introduction of new technologies, human tissue samples are also increasingly being incorporated into drug project screening cascades, including their use in high throughput assays. Improved access to human tissue would undoubtedly further extend the utility of this valuable resource in the drug discovery process.

A Framework for Coordinated Control of Multiagent Systems and Its Applications

In this paper, a framework is proposed for the distributed control and coordination of multiagent systems (MASs). In the proposed framework, the control of MASs is regarded as achieving decentralized control and coordination of agents. Each agent is modeled as a coordinated hybrid agent, which is composed of an intelligent coordination layer and a hybrid control layer. The intelligent coordination layer takes the coordination input, plant input, and workspace input. In the proposed framework, we describe the coordination mechanism in a domain-independent way, i.e., as simple abstract primitives in a coordination rule base for certain dependence relationships between the activities of different agents. The intelligent coordination layer deals with the planning, coordination, decision making, and computation of the agent. The hybrid control layer of the proposed framework takes the output of the intelligent coordination layer and generates discrete and continuous control signals to control the overall process. To verify the feasibility of the proposed framework, experiments for both heterogeneous and homogeneous MASs are implemented. The proposed framework is applied to a multicrane system, a multiple robot system, and a MAS consisting of an overhead crane, a mobile robot, and a robot manipulator. It is demonstrated that the proposed framework can model the three MASs. The agents in these systems are able to cooperate and coordinate to achieve a global goal. In addition, the stability of systems modeled using the proposed framework is also analyzed.

Induction of apoptosis in osteogenic sarcoma cells by combination of tumor necrosis factor-related apoptosis inducing ligand and chemotherapeutic agents

Osteosarcoma is one of the most common primary malignant tumors of bone with poor prognosis. TNF-related apoptosis inducing ligand (TRAIL) is a member of the tumor necrosis factor (TNF) cytokine family. TRAIL induces apoptosis in various tumor cell lines but is not found to be cytotoxic to many normal cell types in vitro. We investigated the cytotoxic activity of TRAIL and chemotherapeutic agents, including methotrexate (MTX), doxorubicin (DOX) and cisplatin (CDDP), on established osteosarcoma cell line--S-732. OS-732 cells were incubated with chemotherapeutic agents MTX, DOX and CDDP at various peak plasma concentrations (PPC), 0.1PPC, 1PPC and 10PPC, alone or with 100 ng/ml of TRAIL for 24 hours or 48 hours. MTT was used to evaluate the cytotoxic activity of different agents on OS-732. The apoptosis proportion was assayed by flow cytometry. Cellular morphologic changes were observed by phase contrast microscope, scan electron microscope, and transmission electron microscope. The inhibitory rate was (24.438 +/- 3.414)% with TRAIL of 100 ng/ml for 24 hours. The cells were responsive to DOX and CDDP with a dose-effect relationship (P < 0.05). In OS-732 cells, DOX and CDDP cooperated synergistically with TRAIL when incubated the cells with them for 24 hours (the combined inhibitory rate is (58.360 +/- 2.146)% and (54.101 +/- 2.721)%, respectively). TRAIL alone or drugs alone induced the apoptosis rate was less than 25% (P < 0.05). However, the combination of TRAIL and MTX did not present synergistic effects on OS-732 cells (P > 0.05, compared with TRAIL alone). Osteosarcoma OS-732 cells were not responsive to TRAIL-induced apoptosis. DOX and CDDP sensitize osteosarcoma OS-732 cells to TRAIL-induced apoptosis. The combination of TRAIL and MTX presented no synergistic effects on killing OS-732 cells.

Direct Effects of Polymyxin B on Human Dendritic Cells Maturation: THE ROLE OF IκB-α/NF-κB AND ERK1/2 PATHWAYS AND ADHESION

Polymyxin B is a lipopolysaccharide binding antibiotic used to inactivate potential lipopolysaccharide contaminations when evaluating the activity of different agents on innate immune cells. We report that polymyxin B is able to induce directly in monocyte-derived human dendritic cells (DCs) several functional and molecular modifications characteristic of DCs undergoing a maturation process. DCs incubated with polymyxin B up-regulate the expression of HLA class I and II, the co-stimulatory CD86 molecule, and show an increase in the fraction of adherent cells at short time, which persist at 48 h of incubation. Adhesion to the plate was required for the polymyxin B-induced DCs maturation. A transient activation of IkappaB-alpha/NF-kappaB and ERK1/2 pathways at short time and a further ERK1/2 activation at long term were also detected. Neither up-regulation of the maturation marker CD83 nor activation of p38 nor induction of cytokines secretion was observed in DCs treated with polymyxin B. We demonstrated that inhibition of IkappaB-alpha/NF-kappaB pathway abolishes polymyxin B effects. ERK1/2 inhibition instead allowed DCs treated with polymyxin B to progress in their maturation process as revealed by the increased up-regulation of the CD83 co-stimulatory molecules, the activation of p38, and the reduced adhesion to culture plates at 48 h of incubation. Our results indicate that polymyxin B induces a partial maturation of human DCs through increased adhesion to a substrate and activation of the IkappaB-alpha/NF-kappaB pathway. The increased ERK1/2 activation observed, even though correlating with the initial phases of the maturation process, actually inhibits the occurrence of full maturation.

Suggested Methods for the Standardisation of the Carcinogenic Activity of Different Agents for the Skin of Mice

Since the discovery of Yamagiwa and Ichikawa that skin cancers could be induced in mice by the repeated application of gas tars, the number of animals utilised for testing the carcinogenic activity of different agents has steadily grown from year to year. Many of the experiments have been performed with a view to ascertaining what factors inhibit and what factors increase or hasten the yield of tumours, but when one attempts to analyse the experimental results available in the literature, it is often a matter of extreme difficulty to correlate the experiments of one worker with those of another. This difficulty is due in part to the variety of methods adopted by many workers when giving expression to their results, but usually in greater part to the inadequacy of the data given by the poor methods generally adopted. It seems a matter of real importance that the results of different workers should be readily comparable one with another, and it is the urgent need for standardisation of results which has stimulated us to make a closer study of this subject. The object of this paper is to place before other cancer research workers some analyses of a variety of experiments on carcinogenesis, in the hope that one or other of the suggested methods, or some modification of them, may find favour, and be generally utilised to express the results of experiments of the class now under consideration.